PMC:3724992 / 12548-13447 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3724992","sourcedb":"PMC","sourceid":"3724992","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3724992","text":"MHC peptide-binding and stability assay\nThe binding affinity and stability of these peptides to HLA-A2.1 was determined by using antigen processing-deficient T2 cells because their enhanced HLA-A2.1. As shown in Table 2, all of the peptides synthesized were bound to HLA-A2.1 molecules but with different affinity and stability. Of four peptides selected, neuritin13–21 up-regulated the HLA-A2.1 molecular expression and showed high affinity and stability to HLA-A2.1, whereas neuritin121–129 and neuritin4–12 showed moderate affinity and neuritin127–135 only had low affinity and stability to the molecule.\nTable 2 HLA-A2-binding affinity and stability of neuritin-derived peptides\nPosition Length Sequence FI DC50\n13–21 9 ILAVQIAYL 1.68 \u003e8\n121–129 9 LLPAFPVLL 1.24 6–8\n4–12 9 KLNGRYISL 1.07 6–8\n127–135 9 VLLVSLSAA 0.68 2–4\n519–526 9 FSYGFFV 0.38 \u003c2\nThe shaded region is the control peptide values","divisions":[{"label":"title","span":{"begin":0,"end":39}},{"label":"label","span":{"begin":608,"end":615}},{"label":"caption","span":{"begin":616,"end":682}},{"label":"p","span":{"begin":616,"end":682}},{"label":"table","span":{"begin":683,"end":851}},{"label":"tr","span":{"begin":683,"end":715}},{"label":"th","span":{"begin":683,"end":691}},{"label":"th","span":{"begin":692,"end":698}},{"label":"th","span":{"begin":699,"end":707}},{"label":"th","span":{"begin":708,"end":710}},{"label":"th","span":{"begin":711,"end":715}},{"label":"tr","span":{"begin":716,"end":741}},{"label":"td","span":{"begin":716,"end":721}},{"label":"td","span":{"begin":722,"end":723}},{"label":"td","span":{"begin":724,"end":733}},{"label":"td","span":{"begin":734,"end":738}},{"label":"td","span":{"begin":739,"end":741}},{"label":"tr","span":{"begin":742,"end":770}},{"label":"td","span":{"begin":742,"end":749}},{"label":"td","span":{"begin":750,"end":751}},{"label":"td","span":{"begin":752,"end":761}},{"label":"td","span":{"begin":762,"end":766}},{"label":"td","span":{"begin":767,"end":770}},{"label":"tr","span":{"begin":771,"end":796}},{"label":"td","span":{"begin":771,"end":775}},{"label":"td","span":{"begin":776,"end":777}},{"label":"td","span":{"begin":778,"end":787}},{"label":"td","span":{"begin":788,"end":792}},{"label":"td","span":{"begin":793,"end":796}},{"label":"tr","span":{"begin":797,"end":825}},{"label":"td","span":{"begin":797,"end":804}},{"label":"td","span":{"begin":805,"end":806}},{"label":"td","span":{"begin":807,"end":816}},{"label":"td","span":{"begin":817,"end":821}},{"label":"td","span":{"begin":822,"end":825}},{"label":"tr","span":{"begin":826,"end":851}},{"label":"td","span":{"begin":826,"end":833}},{"label":"td","span":{"begin":834,"end":835}},{"label":"td","span":{"begin":836,"end":843}},{"label":"td","span":{"begin":844,"end":848}},{"label":"td","span":{"begin":849,"end":851}}],"tracks":[]}