MHC peptide-binding and stability assay
The binding affinity and stability of these peptides to HLA-A2.1 was determined by using antigen processing-deficient T2 cells because their enhanced HLA-A2.1. As shown in Table 2, all of the peptides synthesized were bound to HLA-A2.1 molecules but with different affinity and stability. Of four peptides selected, neuritin13–21 up-regulated the HLA-A2.1 molecular expression and showed high affinity and stability to HLA-A2.1, whereas neuritin121–129 and neuritin4–12 showed moderate affinity and neuritin127–135 only had low affinity and stability to the molecule.
Table 2 HLA-A2-binding affinity and stability of neuritin-derived peptides
Position Length Sequence FI DC50
13–21 9 ILAVQIAYL 1.68 >8
121–129 9 LLPAFPVLL 1.24 6–8
4–12 9 KLNGRYISL 1.07 6–8
127–135 9 VLLVSLSAA 0.68 2–4
519–526 9 FSYGFFV 0.38 <2
The shaded region is the control peptide values