PMC:3681796 / 12753-13558 JSONTXT

{"project":"2_test","denotations":[{"id":"23785402-15263088-91676669","span":{"begin":171,"end":172},"obj":"15263088"}],"text":"Indirect calorimetry\nO2 consumption and CO2 production were measured using an open-circuit indirect calorimetry system (Klogor, Lannion, France), as previously described [9]. Concentrations of O2 and CO2 in the outgoing air were successively measured in five different cages. The system was rinsed for 90 s between each measurement. Final values of gas concentrations were the mean of 10 measures obtained during 40 s. Each cage was sampled every 11 min, and one cage was left vacant as reference of ambient gas concentrations. Measurements were performed continuously over 23 hours and a half, a 30-min period being required for calibration of the O2 and CO2 analyzers. In total, 127 measures were collected per day and mouse. The respiratory quotient was the ratio of CO2 production over O2 consumption."}