PMC:3654953 / 16185-17120 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3654953","sourcedb":"PMC","sourceid":"3654953","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3654953","text":"Characterization of the GFAP c.1289G\u003eA/p.R430H mutation. A: Schematic representation of the exonic structure of different GFAP isoforms. Dotted lines indicate the termination codons. The arrows indicate the position of the c.1289G\u003eA variant (Note that in GFAP-κ the c.1289G\u003eA mutation is part of the 3′-UTR). B: Electropherograms of GFAP exon 7A region containing c.1289G\u003eA variant, in patients 1 and 2 (Pt1, Pt2) and in their mother (I-2). C: The histogram displays the percentages of cells transfected with GFP-GFAP-ϵwt (green bars) or GFP-GFAP-ϵR430H (purple bars), classified in filamentous pattern (F), cytoplasmic aggregates on a filamentous pattern (F + A), cytoplasmic aggregates with no filamentous pattern (A). Scale bars represent 15 μm. A total of 324 cells for GFP-GFAP-ϵwt and 285 for GFP-GFAP-ϵR430H, from 3 independent experiments, were blindly analyzed by two different operators. ANOVA test for interaction p = 0.001.","tracks":[]}