PMC:3654953 / 12659-13436 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3654953","sourcedb":"PMC","sourceid":"3654953","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3654953","text":"Cell culture, transient transfections, western-blot analysis, and immunocytochemistry were performed as described, [12-15] using antibodies against α-tubulin (Life Science) and acetylated α-tubulin (Sigma). Patients’ fibroblasts and adult control fibroblasts were grown under the same conditions, and analyzed among culture passages 5 and 8. As a positive control for tubulin acetylation, fibroblasts were pre-incubated with the specific HDAC6 inhibitor Tubacin (0, 0.2 μM and 2.5 μM) (Sigma) for 24 h [16]. Immunohistochemistry was carried out on 2 μm thick sections from pellets of Pt1, Pt2 and control fibroblasts, fixed in glutaraldehyde 2.5% (Electron Microscopy Science - EMS), in 0.05 M PBS pH 7.4, dehydrated in graded acetone, and embedded in Spurr (Epoxy resin, EMS).","tracks":[{"project":"2_test","denotations":[{"id":"23634874-10556302-81641079","span":{"begin":116,"end":118},"obj":"10556302"},{"id":"23634874-19124534-81641079","span":{"begin":116,"end":118},"obj":"19124534"},{"id":"23634874-18197187-81641079","span":{"begin":116,"end":118},"obj":"18197187"},{"id":"23634874-15763182-81641079","span":{"begin":116,"end":118},"obj":"15763182"},{"id":"23634874-12677000-81641080","span":{"begin":503,"end":505},"obj":"12677000"},{"id":"T35443","span":{"begin":116,"end":118},"obj":"10556302"},{"id":"T86558","span":{"begin":116,"end":118},"obj":"19124534"},{"id":"T83265","span":{"begin":116,"end":118},"obj":"18197187"},{"id":"T91806","span":{"begin":116,"end":118},"obj":"15763182"},{"id":"T35368","span":{"begin":503,"end":505},"obj":"12677000"}],"attributes":[{"subj":"23634874-10556302-81641079","pred":"source","obj":"2_test"},{"subj":"23634874-19124534-81641079","pred":"source","obj":"2_test"},{"subj":"23634874-18197187-81641079","pred":"source","obj":"2_test"},{"subj":"23634874-15763182-81641079","pred":"source","obj":"2_test"},{"subj":"23634874-12677000-81641080","pred":"source","obj":"2_test"},{"subj":"T35443","pred":"source","obj":"2_test"},{"subj":"T86558","pred":"source","obj":"2_test"},{"subj":"T83265","pred":"source","obj":"2_test"},{"subj":"T91806","pred":"source","obj":"2_test"},{"subj":"T35368","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec93c7","default":true}]}]}}