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PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. 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PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T4782","span":{"begin":152,"end":160},"obj":"Protein"},{"id":"T4795","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T4794","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T4793","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T4792","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T4791","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T4790","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T4789","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T4788","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T4787","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T4786","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T4785","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T4784","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T4783","span":{"begin":370,"end":378},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5245","span":{"begin":472,"end":482},"obj":"http://www.uniprot.org/uniprot/P08659"},{"id":"T5242","span":{"begin":938,"end":946},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T5241","span":{"begin":463,"end":471},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T5240","span":{"begin":370,"end":378},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T5239","span":{"begin":152,"end":160},"obj":"http://www.uniprot.org/uniprot/P05120"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"sentences","denotations":[{"id":"T4746","span":{"begin":1060,"end":1110},"obj":"Sentence"},{"id":"T4745","span":{"begin":870,"end":1059},"obj":"Sentence"},{"id":"T4744","span":{"begin":445,"end":869},"obj":"Sentence"},{"id":"T4743","span":{"begin":295,"end":444},"obj":"Sentence"},{"id":"T4742","span":{"begin":0,"end":294},"obj":"Sentence"},{"id":"T58","span":{"begin":0,"end":294},"obj":"Sentence"},{"id":"T59","span":{"begin":295,"end":444},"obj":"Sentence"},{"id":"T60","span":{"begin":445,"end":869},"obj":"Sentence"},{"id":"T61","span":{"begin":870,"end":1059},"obj":"Sentence"},{"id":"T62","span":{"begin":1060,"end":1110},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"simple1","denotations":[{"id":"T4815","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T4814","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T4813","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T4812","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T4811","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T4810","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T4809","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T4808","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T4807","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T4806","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T4805","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T4804","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T4803","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T4802","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T5232","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T5231","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T5230","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T5229","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T5228","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T5227","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T5226","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T5225","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T5224","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T5223","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T5222","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T5221","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T5220","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T5219","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T5303","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T5302","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T5301","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T5300","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T5299","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T5298","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T5297","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T5296","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T5295","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T5294","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T5293","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T5292","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T5291","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T5290","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"DLUT931","denotations":[{"id":"T5262","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T5261","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T5260","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T5259","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T5258","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T5257","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T5256","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T5255","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T5254","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T5253","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T5252","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T5251","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T5250","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T5249","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T5482","span":{"begin":794,"end":817},"obj":"Protein"},{"id":"T5481","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T5480","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T5479","span":{"begin":463,"end":502},"obj":"Protein"},{"id":"T5478","span":{"begin":528,"end":531},"obj":"Protein"},{"id":"T5477","span":{"begin":927,"end":963},"obj":"Entity"},{"id":"T5476","span":{"begin":412,"end":416},"obj":"Protein"},{"id":"T5472","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T5468","span":{"begin":126,"end":129},"obj":"Protein"},{"id":"T5467","span":{"begin":256,"end":271},"obj":"Protein"},{"id":"T5466","span":{"begin":504,"end":505},"obj":"Entity"},{"id":"T5464","span":{"begin":299,"end":305},"obj":"Protein"},{"id":"T5463","span":{"begin":517,"end":521},"obj":"Protein"},{"id":"T5462","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T5456","span":{"begin":411,"end":443},"obj":"Entity"},{"id":"T5455","span":{"begin":552,"end":553},"obj":"Entity"},{"id":"T5451","span":{"begin":284,"end":288},"obj":"Protein"},{"id":"T5450","span":{"begin":36,"end":60},"obj":"Entity"},{"id":"T5449","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T5446","span":{"begin":563,"end":568},"obj":"Protein"},{"id":"T5445","span":{"begin":366,"end":407},"obj":"Entity"},{"id":"T5444","span":{"begin":434,"end":438},"obj":"Protein"},{"id":"T5443","span":{"begin":152,"end":160},"obj":"Protein"},{"id":"T5440","span":{"begin":1089,"end":1093},"obj":"Protein"},{"id":"T5439","span":{"begin":366,"end":407},"obj":"Entity"},{"id":"T5437","span":{"begin":1033,"end":1058},"obj":"Protein"},{"id":"T5435","span":{"begin":433,"end":443},"obj":"Entity"},{"id":"T5434","span":{"begin":516,"end":517},"obj":"Entity"},{"id":"T5433","span":{"begin":388,"end":394},"obj":"Protein"},{"id":"T5431","span":{"begin":89,"end":94},"obj":"Protein"},{"id":"T5430","span":{"begin":855,"end":857},"obj":"Entity"},{"id":"T5427","span":{"begin":4,"end":7},"obj":"Protein"},{"id":"T5425","span":{"begin":616,"end":621},"obj":"Protein"},{"id":"T5424","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T5422","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T5421","span":{"begin":1089,"end":1109},"obj":"Protein"},{"id":"T5420","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T5417","span":{"begin":874,"end":877},"obj":"Protein"},{"id":"T5416","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T5414","span":{"begin":664,"end":678},"obj":"Protein"},{"id":"T5413","span":{"begin":553,"end":557},"obj":"Protein"},{"id":"T5411","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T5407","span":{"begin":148,"end":184},"obj":"Entity"},{"id":"T5405","span":{"begin":283,"end":293},"obj":"Entity"},{"id":"T5403","span":{"begin":505,"end":509},"obj":"Protein"},{"id":"T5401","span":{"begin":577,"end":582},"obj":"Protein"},{"id":"T5399","span":{"begin":463,"end":471},"obj":"Protein"},{"id":"T5397","span":{"begin":632,"end":727},"obj":"Protein"},{"id":"T5395","span":{"begin":794,"end":796},"obj":"Entity"},{"id":"T5393","span":{"begin":532,"end":545},"obj":"Protein"},{"id":"T5390","span":{"begin":813,"end":816},"obj":"Protein"}],"relations":[{"id":"R3841","pred":"partOf","subj":"T5407","obj":"T5443"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T5329","span":{"begin":1042,"end":1049},"obj":"Gene_expression"},{"id":"T5328","span":{"begin":1028,"end":1032},"obj":"Protein"},{"id":"T5327","span":{"begin":984,"end":995},"obj":"Protein"},{"id":"T5326","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T5325","span":{"begin":813,"end":816},"obj":"Protein"},{"id":"T5324","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T5323","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T5322","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T5321","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T5320","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T5319","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T5318","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T5317","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T5316","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T5315","span":{"begin":456,"end":491},"obj":"Protein"},{"id":"T5314","span":{"begin":425,"end":432},"obj":"Gene_expression"},{"id":"T5313","span":{"begin":299,"end":305},"obj":"Protein"},{"id":"T5312","span":{"begin":251,"end":255},"obj":"Protein"},{"id":"T5311","span":{"begin":207,"end":218},"obj":"Protein"},{"id":"T5310","span":{"begin":38,"end":43},"obj":"Protein"}],"relations":[{"id":"R3837","pred":"themeOf","subj":"T5313","obj":"T5314"},{"id":"R3838","pred":"themeOf","subj":"T5328","obj":"T5329"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"testone","denotations":[{"id":"T4698","span":{"begin":1042,"end":1049},"obj":"Gene_expression"},{"id":"T4697","span":{"begin":425,"end":432},"obj":"Gene_expression"},{"id":"T4696","span":{"begin":275,"end":282},"obj":"Gene_expression"},{"id":"T4690","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T4689","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T4688","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T4687","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T4686","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T4685","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T4684","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T4683","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T4682","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T4681","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T4680","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T4679","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T4678","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T4677","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}

{"project":"test3","denotations":[{"id":"T4735","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T4734","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T4733","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T4732","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T4731","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T4730","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T4729","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T4728","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T4727","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T4726","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T4725","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T4724","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T4723","span":{"begin":425,"end":432},"obj":"Gene_expression"},{"id":"T4722","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T4721","span":{"begin":275,"end":282},"obj":"Gene_expression"},{"id":"T4720","span":{"begin":152,"end":160},"obj":"Protein"},{"id":"T4713","span":{"begin":938,"end":946},"obj":"Protein"},{"id":"T4712","span":{"begin":855,"end":868},"obj":"Protein"},{"id":"T4711","span":{"begin":794,"end":811},"obj":"Protein"},{"id":"T4710","span":{"begin":722,"end":727},"obj":"Protein"},{"id":"T4709","span":{"begin":710,"end":717},"obj":"Protein"},{"id":"T4708","span":{"begin":703,"end":708},"obj":"Protein"},{"id":"T4707","span":{"begin":694,"end":701},"obj":"Protein"},{"id":"T4706","span":{"begin":686,"end":692},"obj":"Protein"},{"id":"T4705","span":{"begin":679,"end":684},"obj":"Protein"},{"id":"T4704","span":{"begin":672,"end":677},"obj":"Protein"},{"id":"T4703","span":{"begin":632,"end":638},"obj":"Protein"},{"id":"T4702","span":{"begin":463,"end":491},"obj":"Protein"},{"id":"T4701","span":{"begin":370,"end":378},"obj":"Protein"},{"id":"T4700","span":{"begin":152,"end":160},"obj":"Protein"}],"text":"The PCR primers DW5’LUC, containing a Kpn I restriction site, and DW3’LUC, containing an Xho I restriction site, were used to PCR amplify and clone the SerpinB2 promoter (−3261 to +92) from pDB9406 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic (Promega) to produce pGLmP-3261. The EcoR I insert of pDB9402-42 was sub-cloned immediately upstream of the SerpinB2 promoter EcoR I site (−3261) of pGLmP-3261 to produce pGLmP-4480. Additional murine SerpinB2 luciferase reporter constructs (pGLmP-2751, pGLmP-2614, pGLmP-1686, pGLmP-1341, pGLmP-694, pGLmP-539 and pGLmP-189) were generated by digesting pGLmP-3261 with EcoR I and a second restriction enzyme (Sfi I, Apa I, BstX I, Bsu36 I, Pac I, Hae III and Apo I respectively), blunt ending the resultant 5′ or 3′ overhangs with T4 DNA polymerase (NEB) and re-ligating the vector ends with T4 DNA ligase. The PCR primers BSPCR2 and DW3’LUC were used to subclone the murine SerpinB2 promoter regions −87 to +92 into the Kpn I/Xho I polylinker restriction sites of pGL3 Basic to produce pGLmP-87. The control empty vector was pGL3 Basic (Promega)."}