PMC:3589482 / 8267-9039 JSON TXT

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bionlp-st-ge-2016-spacy-parsed

2_test

{"project":"2_test","denotations":[{"id":"23472114-9892694-87899173","span":{"begin":566,"end":568},"obj":"9892694"},{"id":"T16742","span":{"begin":566,"end":568},"obj":"9892694"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T4273","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4272","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4271","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4270","span":{"begin":504,"end":538},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4445","span":{"begin":715,"end":720},"obj":"http://www.uniprot.org/uniprot/P04406"},{"id":"T4444","span":{"begin":688,"end":713},"obj":"http://www.uniprot.org/uniprot/P04406"},{"id":"T4443","span":{"begin":626,"end":633},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T4442","span":{"begin":515,"end":523},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T4441","span":{"begin":423,"end":431},"obj":"http://www.uniprot.org/uniprot/P05120"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T4259","span":{"begin":299,"end":303},"obj":"http://purl.obolibrary.org/obo/UBERON_0001913"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T4276","span":{"begin":577,"end":587},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T4275","span":{"begin":432,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T4274","span":{"begin":365,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T4285","span":{"begin":577,"end":587},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T4284","span":{"begin":432,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T4283","span":{"begin":365,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T4282","span":{"begin":577,"end":587},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T4281","span":{"begin":432,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T4280","span":{"begin":365,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T4279","span":{"begin":255,"end":264},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T4278","span":{"begin":244,"end":253},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T4277","span":{"begin":26,"end":30},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

sentences

{"project":"sentences","denotations":[{"id":"T4264","span":{"begin":571,"end":772},"obj":"Sentence"},{"id":"T4263","span":{"begin":387,"end":570},"obj":"Sentence"},{"id":"T4262","span":{"begin":255,"end":386},"obj":"Sentence"},{"id":"T4261","span":{"begin":20,"end":254},"obj":"Sentence"},{"id":"T4260","span":{"begin":0,"end":19},"obj":"Sentence"},{"id":"T52","span":{"begin":0,"end":19},"obj":"Sentence"},{"id":"T53","span":{"begin":20,"end":254},"obj":"Sentence"},{"id":"T54","span":{"begin":255,"end":386},"obj":"Sentence"},{"id":"T55","span":{"begin":387,"end":570},"obj":"Sentence"},{"id":"T56","span":{"begin":571,"end":772},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

simple1

{"project":"simple1","denotations":[{"id":"T4293","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4292","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4291","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4290","span":{"begin":504,"end":538},"obj":"Protein"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T4440","span":{"begin":539,"end":547},"obj":"Gene_expression"},{"id":"T4439","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4438","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4437","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4436","span":{"begin":504,"end":538},"obj":"Protein"}],"relations":[{"id":"R3281","pred":"themeOf","subj":"T4436","obj":"T4440"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T4460","span":{"begin":539,"end":547},"obj":"Gene_expression"},{"id":"T4459","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4458","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4457","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4456","span":{"begin":504,"end":538},"obj":"Protein"}],"relations":[{"id":"R3285","pred":"themeOf","subj":"T4456","obj":"T4460"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T4455","span":{"begin":539,"end":547},"obj":"Gene_expression"},{"id":"T4454","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4453","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4452","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4451","span":{"begin":504,"end":538},"obj":"Protein"}],"relations":[{"id":"R3284","pred":"themeOf","subj":"T4451","obj":"T4455"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T4500","span":{"begin":481,"end":558},"obj":"Gene_expression"},{"id":"T4499","span":{"begin":81,"end":92},"obj":"Entity"},{"id":"T4498","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4497","span":{"begin":166,"end":193},"obj":"Entity"},{"id":"T4496","span":{"begin":726,"end":737},"obj":"Protein"},{"id":"T4495","span":{"begin":141,"end":149},"obj":"Protein"},{"id":"T4494","span":{"begin":239,"end":253},"obj":"Entity"},{"id":"T4493","span":{"begin":122,"end":150},"obj":"Entity"},{"id":"T4492","span":{"begin":739,"end":743},"obj":"Entity"},{"id":"T4491","span":{"begin":673,"end":721},"obj":"Protein"},{"id":"T4490","span":{"begin":69,"end":114},"obj":"Entity"},{"id":"T4489","span":{"begin":597,"end":616},"obj":"Entity"},{"id":"T4488","span":{"begin":307,"end":321},"obj":"Entity"},{"id":"T4487","span":{"begin":626,"end":642},"obj":"Protein"},{"id":"T4486","span":{"begin":239,"end":243},"obj":"Entity"},{"id":"T4485","span":{"begin":75,"end":93},"obj":"Entity"},{"id":"T4484","span":{"begin":481,"end":558},"obj":"Protein"},{"id":"T4483","span":{"begin":255,"end":264},"obj":"Entity"},{"id":"T4482","span":{"begin":201,"end":218},"obj":"Entity"},{"id":"T4481","span":{"begin":152,"end":160},"obj":"Protein"},{"id":"T4480","span":{"begin":423,"end":442},"obj":"Entity"},{"id":"T4479","span":{"begin":423,"end":431},"obj":"Protein"},{"id":"T4478","span":{"begin":357,"end":375},"obj":"Entity"},{"id":"T4477","span":{"begin":571,"end":616},"obj":"Entity"}],"relations":[{"id":"R3290","pred":"themeOf","subj":"T4484","obj":"T4500"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T4468","span":{"begin":726,"end":737},"obj":"Protein"},{"id":"T4467","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4466","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4465","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4464","span":{"begin":539,"end":547},"obj":"Gene_expression"},{"id":"T4463","span":{"begin":539,"end":547},"obj":"Gene_expression"},{"id":"T4462","span":{"begin":508,"end":538},"obj":"Protein"},{"id":"T4461","span":{"begin":504,"end":507},"obj":"Protein"}],"relations":[{"id":"R3286","pred":"themeOf","subj":"T4461","obj":"T4463"},{"id":"R3287","pred":"themeOf","subj":"T4462","obj":"T4464"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

testone

{"project":"testone","denotations":[{"id":"T4250","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4249","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4248","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4247","span":{"begin":504,"end":538},"obj":"Protein"}],"relations":[{"id":"R3134","pred":"equivalentTo","subj":"T4250","obj":"T4249"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

test3

{"project":"test3","denotations":[{"id":"T4258","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4257","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4256","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4255","span":{"begin":504,"end":538},"obj":"Protein"},{"id":"T4254","span":{"begin":715,"end":720},"obj":"Protein"},{"id":"T4253","span":{"begin":673,"end":713},"obj":"Protein"},{"id":"T4252","span":{"begin":626,"end":633},"obj":"Protein"},{"id":"T4251","span":{"begin":504,"end":538},"obj":"Protein"}],"relations":[{"id":"R3135","pred":"equivalentTo","subj":"T4258","obj":"T4257"}],"text":"Western Blot Assays\nWhole cell lysates were prepared in RIPA buffer (10 mM Tris, 150 mM NaCl, 1%Triton X-100, 0.5% NP-40, 0.5% deoxycholate, 0.1% SDS), proteins were separated on 4–12% Bis-Tris NuPAGE gels (Invitrogen), and transferred to PVDF membranes. Membranes were subsequently blocked with 5% milk in PBS-T (1X PBS, 0.1% Tween-20), and incubated with primary antibodies overnight. Affinity purified rabbit anti-mouse SerpinB2 antibodies were prepared after immunization with a purified recombinant GST-murine SerpinB2 fusion protein produced in E. coli as in [35]. Other antibodies used for western blot assays include: C/EBP-β (sc-150) (Santa Cruz Biotechnologies), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and α/β tubulin (Cell Signaling Technology, Inc.)."}

PMC:3589482 / 8267-9039 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3589482 / 8267-9039 JSON TXT