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investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

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investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T14036","span":{"begin":1059,"end":1067},"obj":"Protein"},{"id":"T14035","span":{"begin":966,"end":973},"obj":"Protein"},{"id":"T14034","span":{"begin":884,"end":891},"obj":"Protein"},{"id":"T14033","span":{"begin":828,"end":836},"obj":"Protein"},{"id":"T14032","span":{"begin":675,"end":682},"obj":"Protein"},{"id":"T14031","span":{"begin":526,"end":531},"obj":"Protein"},{"id":"T14030","span":{"begin":471,"end":479},"obj":"Protein"},{"id":"T14029","span":{"begin":394,"end":402},"obj":"Protein"},{"id":"T14028","span":{"begin":338,"end":346},"obj":"Protein"},{"id":"T14027","span":{"begin":308,"end":315},"obj":"Protein"},{"id":"T14026","span":{"begin":280,"end":288},"obj":"Protein"},{"id":"T14025","span":{"begin":223,"end":230},"obj":"Protein"},{"id":"T14024","span":{"begin":157,"end":162},"obj":"Protein"},{"id":"T14023","span":{"begin":126,"end":131},"obj":"Protein"},{"id":"T14022","span":{"begin":112,"end":119},"obj":"Protein"},{"id":"T14021","span":{"begin":55,"end":63},"obj":"Protein"},{"id":"T14020","span":{"begin":33,"end":40},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T14300","span":{"begin":884,"end":891},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14299","span":{"begin":675,"end":682},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14298","span":{"begin":308,"end":315},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14297","span":{"begin":223,"end":230},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14296","span":{"begin":112,"end":119},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14295","span":{"begin":33,"end":40},"obj":"http://www.uniprot.org/uniprot/P28033"},{"id":"T14309","span":{"begin":1059,"end":1067},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14308","span":{"begin":828,"end":836},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14307","span":{"begin":471,"end":479},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14306","span":{"begin":394,"end":402},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14305","span":{"begin":338,"end":346},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14304","span":{"begin":280,"end":288},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14303","span":{"begin":55,"end":63},"obj":"http://www.uniprot.org/uniprot/P05120"},{"id":"T14301","span":{"begin":966,"end":973},"obj":"http://www.uniprot.org/uniprot/P28033"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"GO-BP","denotations":[{"id":"T14038","span":{"begin":1031,"end":1044},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T14037","span":{"begin":83,"end":98},"obj":"http://purl.obolibrary.org/obo/GO_0032496"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"GO-CC","denotations":[{"id":"T14039","span":{"begin":183,"end":188},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"sentences","denotations":[{"id":"T13966","span":{"begin":945,"end":1073},"obj":"Sentence"},{"id":"T13965","span":{"begin":769,"end":944},"obj":"Sentence"},{"id":"T13964","span":{"begin":566,"end":768},"obj":"Sentence"},{"id":"T13963","span":{"begin":364,"end":565},"obj":"Sentence"},{"id":"T13962","span":{"begin":232,"end":363},"obj":"Sentence"},{"id":"T13961","span":{"begin":0,"end":231},"obj":"Sentence"},{"id":"T190","span":{"begin":0,"end":231},"obj":"Sentence"},{"id":"T191","span":{"begin":232,"end":363},"obj":"Sentence"},{"id":"T192","span":{"begin":364,"end":565},"obj":"Sentence"},{"id":"T193","span":{"begin":566,"end":768},"obj":"Sentence"},{"id":"T194","span":{"begin":769,"end":944},"obj":"Sentence"},{"id":"T195","span":{"begin":945,"end":1073},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

{"project":"simple1","denotations":[{"id":"T14058","span":{"begin":1059,"end":1067},"obj":"Protein"},{"id":"T14057","span":{"begin":966,"end":973},"obj":"Protein"},{"id":"T14056","span":{"begin":884,"end":891},"obj":"Protein"},{"id":"T14055","span":{"begin":828,"end":836},"obj":"Protein"},{"id":"T14054","span":{"begin":675,"end":682},"obj":"Protein"},{"id":"T14053","span":{"begin":526,"end":531},"obj":"Protein"},{"id":"T14052","span":{"begin":471,"end":479},"obj":"Protein"},{"id":"T14051","span":{"begin":394,"end":402},"obj":"Protein"},{"id":"T14050","span":{"begin":338,"end":346},"obj":"Protein"},{"id":"T14049","span":{"begin":308,"end":315},"obj":"Protein"},{"id":"T14048","span":{"begin":280,"end":288},"obj":"Protein"},{"id":"T14047","span":{"begin":223,"end":230},"obj":"Protein"},{"id":"T14046","span":{"begin":157,"end":162},"obj":"Protein"},{"id":"T14045","span":{"begin":126,"end":131},"obj":"Protein"},{"id":"T14044","span":{"begin":112,"end":119},"obj":"Protein"},{"id":"T14043","span":{"begin":55,"end":63},"obj":"Protein"},{"id":"T14042","span":{"begin":33,"end":40},"obj":"Protein"}],"text":"To investigate the importance of C/EBP-β to endogenous SerpinB2 mRNA expression in response to LPS, we utilized C/EBP-β-null (Cebpb −/−) and wild-type MEFs (Cebpb+/+), since RAW264.7 cells constitutively express endogenous C/EBP-β. Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

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Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

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Wild-type MEFs express low levels of endogenous SerpinB2 and the absence of C/EBP-β attenuated endogenous SerpinB2 mRNA expression. LPS stimulated an increase in SerpinB2 mRNA expression in wild-type MEFs (Fig. 6A), whereas LPS-stimulated SerpinB2 mRNA expression was significantly dampened in Cebpb−/− MEFs as compared to wild-type. We next tested if similar effects could be seen in thioglycollate-elicited inflammatory macrophages in which C/EBP-β expression was knocked down using species-specific lentiviral shRNAs (Fig. 6B, left). As was observed in MEFs, both constitutive and LPS-induced SerpinB2 mRNA expression was significantly decreased in C/EBP-β-deficient inflammatory macrophages (Fig. 6B, right). These data show that C/EBP-β is critical for mediating constitutive and LPS-inducible transcription of endogenous SerpinB2 mRNA."}

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