PMC:3585731 / 57278-57674
Annnotations
testone
{"project":"testone","denotations":[{"id":"T28276","span":{"begin":323,"end":338},"obj":"Phosphorylation"},{"id":"T28275","span":{"begin":149,"end":156},"obj":"Protein"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
pmc-enju-pas
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,{"id":"R19630","pred":"arg3Of","subj":"T28314","obj":"T28312"},{"id":"R19631","pred":"arg2Of","subj":"T28318","obj":"T28315"},{"id":"R19632","pred":"arg1Of","subj":"T28318","obj":"T28316"},{"id":"R19633","pred":"arg1Of","subj":"T28318","obj":"T28317"},{"id":"R19634","pred":"arg1Of","subj":"T28318","obj":"T28319"},{"id":"R19635","pred":"arg2Of","subj":"T28320","obj":"T28319"},{"id":"R19636","pred":"arg1Of","subj":"T28322","obj":"T28323"},{"id":"R19637","pred":"arg2Of","subj":"T28322","obj":"T28324"},{"id":"R19638","pred":"modOf","subj":"T28324","obj":"T28304"},{"id":"R19639","pred":"arg1Of","subj":"T28324","obj":"T28321"},{"id":"R19640","pred":"arg2Of","subj":"T28324","obj":"T28323"},{"id":"R19641","pred":"arg1Of","subj":"T28324","obj":"T28325"},{"id":"R19642","pred":"arg2Of","subj":"T28327","obj":"T28325"},{"id":"R19643","pred":"arg1Of","subj":"T28327","obj":"T28326"},{"id":"R19644","pred":"arg1Of","subj":"T28327","obj":"T28328"},{"id":"R19645","pred":"arg2Of","subj":"T28332","obj":"T28328"},{"id":"R19646","pred":"arg1Of","subj":"T28332","obj":"T28329"},{"id":"R19647","pred":"arg1Of","subj":"T28332","obj":"T28330"},{"id":"R19648","pred":"arg1Of","subj":"T28332","obj":"T28331"},{"id":"R19649","pred":"arg1Of","subj":"T28332","obj":"T28333"},{"id":"R19650","pred":"arg2Of","subj":"T28334","obj":"T28333"},{"id":"R19651","pred":"arg3Of","subj":"T28335","obj":"T28333"},{"id":"R19652","pred":"arg1Of","subj":"T28338","obj":"T28337"},{"id":"R19653","pred":"arg1Of","subj":"T28339","obj":"T28336"},{"id":"R19654","pred":"arg1Of","subj":"T28339","obj":"T28338"},{"id":"R19655","pred":"arg1Of","subj":"T28339","obj":"T28340"},{"id":"R19656","pred":"arg2Of","subj":"T28339","obj":"T28341"},{"id":"R19657","pred":"arg2Of","subj":"T28341","obj":"T28340"},{"id":"R19658","pred":"arg1Of","subj":"T28341","obj":"T28342"},{"id":"R19659","pred":"arg2Of","subj":"T28344","obj":"T28342"},{"id":"R19660","pred":"arg1Of","subj":"T28344","obj":"T28343"},{"id":"R19661","pred":"arg1Of","subj":"T28344","obj":"T28345"},{"id":"R19662","pred":"arg2Of","subj":"T28350","obj":"T28345"},{"id":"R19663","pred":"arg1Of","subj":"T28350","obj":"T28346"},{"id":"R19664","pred":"arg1Of","subj":"T28350","obj":"T28347"},{"id":"R19665","pred":"arg1Of","subj":"T28350","obj":"T28348"},{"id":"R19666","pred":"arg1Of","subj":"T28350","obj":"T28349"},{"id":"R19667","pred":"arg1Of","subj":"T28351","obj":"T28352"},{"id":"R19668","pred":"arg1Of","subj":"T28351","obj":"T28357"},{"id":"R19669","pred":"arg2Of","subj":"T28351","obj":"T28358"},{"id":"R19670","pred":"arg2Of","subj":"T28356","obj":"T28352"},{"id":"R19671","pred":"arg1Of","subj":"T28356","obj":"T28353"},{"id":"R19672","pred":"arg1Of","subj":"T28356","obj":"T28354"},{"id":"R19673","pred":"arg1Of","subj":"T28356","obj":"T28355"},{"id":"R19674","pred":"arg2Of","subj":"T28358","obj":"T28357"},{"id":"R19675","pred":"arg1Of","subj":"T28361","obj":"T28358"},{"id":"R19676","pred":"arg2Of","subj":"T28361","obj":"T28359"},{"id":"R19677","pred":"arg1Of","subj":"T28361","obj":"T28360"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
bionlp-st-ge-2016-test-proteins
{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T28284","span":{"begin":149,"end":156},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T28375","span":{"begin":153,"end":156},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T28374","span":{"begin":69,"end":72},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T28373","span":{"begin":26,"end":29},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T28372","span":{"begin":9,"end":12},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T28371","span":{"begin":153,"end":156},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T28370","span":{"begin":69,"end":72},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T28369","span":{"begin":26,"end":29},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T28368","span":{"begin":9,"end":12},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T28367","span":{"begin":153,"end":156},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T28366","span":{"begin":69,"end":72},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T28365","span":{"begin":26,"end":29},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T28364","span":{"begin":9,"end":12},"obj":"http://www.uniprot.org/uniprot/P31749"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T28288","span":{"begin":323,"end":338},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T28287","span":{"begin":346,"end":349},"obj":"http://purl.obolibrary.org/obo/GO_0050321"},{"id":"T28286","span":{"begin":293,"end":296},"obj":"http://purl.obolibrary.org/obo/GO_0050321"},{"id":"T28285","span":{"begin":30,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0016301"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T28291","span":{"begin":346,"end":349},"obj":"http://purl.obolibrary.org/obo/GO_0050321"},{"id":"T28290","span":{"begin":293,"end":296},"obj":"http://purl.obolibrary.org/obo/GO_0050321"},{"id":"T28289","span":{"begin":30,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0016301"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T28293","span":{"begin":190,"end":195},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28292","span":{"begin":112,"end":116},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
sentences
{"project":"sentences","denotations":[{"id":"T28282","span":{"begin":323,"end":396},"obj":"Sentence"},{"id":"T28281","span":{"begin":239,"end":322},"obj":"Sentence"},{"id":"T28280","span":{"begin":26,"end":238},"obj":"Sentence"},{"id":"T28279","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T375","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T376","span":{"begin":26,"end":138},"obj":"Sentence"},{"id":"T377","span":{"begin":139,"end":238},"obj":"Sentence"},{"id":"T378","span":{"begin":239,"end":322},"obj":"Sentence"},{"id":"T379","span":{"begin":323,"end":396},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
simple1
{"project":"simple1","denotations":[{"id":"T28295","span":{"begin":149,"end":156},"obj":"Protein"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
BioNLP16_DUT
{"project":"BioNLP16_DUT","denotations":[{"id":"T28363","span":{"begin":161,"end":179},"obj":"Binding"},{"id":"T28362","span":{"begin":149,"end":156},"obj":"Protein"}],"relations":[{"id":"R19678","pred":"themeOf","subj":"T28362","obj":"T28363"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
BioNLP16_Messiy
{"project":"BioNLP16_Messiy","denotations":[{"id":"T28380","span":{"begin":161,"end":179},"obj":"Binding"},{"id":"T28379","span":{"begin":149,"end":156},"obj":"Protein"}],"relations":[{"id":"R19680","pred":"themeOf","subj":"T28379","obj":"T28380"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
DLUT931
{"project":"DLUT931","denotations":[{"id":"T28377","span":{"begin":161,"end":179},"obj":"Binding"},{"id":"T28376","span":{"begin":149,"end":156},"obj":"Protein"}],"relations":[{"id":"R19679","pred":"themeOf","subj":"T28376","obj":"T28377"}],"text":"In vitro Akt Kinase Assay\nAkt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma). The in vitro assay was performed in the presence of a GSK fusion protein substrate. Phosphorylation of the GSK fusion protein was visualized by western blot."}
bionlp-st-ge-2016-test-ihmc
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bionlp-st-ge-2016-spacy-parsed
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bionlp-st-ge-2016-test-tees
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test3
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