PMC:3585731 / 56076-56510 JSON TXT

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testone

{"project":"testone","denotations":[{"id":"T27772","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T27779","span":{"begin":20,"end":28},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T27781","span":{"begin":347,"end":352},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T27780","span":{"begin":69,"end":74},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

sentences

{"project":"sentences","denotations":[{"id":"T27777","span":{"begin":377,"end":434},"obj":"Sentence"},{"id":"T27776","span":{"begin":245,"end":376},"obj":"Sentence"},{"id":"T27775","span":{"begin":29,"end":244},"obj":"Sentence"},{"id":"T27774","span":{"begin":0,"end":28},"obj":"Sentence"},{"id":"T361","span":{"begin":0,"end":28},"obj":"Sentence"},{"id":"T362","span":{"begin":29,"end":244},"obj":"Sentence"},{"id":"T363","span":{"begin":245,"end":376},"obj":"Sentence"},{"id":"T364","span":{"begin":377,"end":434},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

simple1

{"project":"simple1","denotations":[{"id":"T27783","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T27858","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T27861","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T27859","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T27877","span":{"begin":0,"end":28},"obj":"Regulation"},{"id":"T27876","span":{"begin":110,"end":127},"obj":"Entity"},{"id":"T27875","span":{"begin":415,"end":433},"obj":"Entity"},{"id":"T27874","span":{"begin":60,"end":87},"obj":"Entity"},{"id":"T27873","span":{"begin":377,"end":382},"obj":"Entity"},{"id":"T27872","span":{"begin":200,"end":243},"obj":"Entity"},{"id":"T27871","span":{"begin":17,"end":28},"obj":"Protein"},{"id":"T27870","span":{"begin":115,"end":127},"obj":"Entity"},{"id":"T27869","span":{"begin":358,"end":375},"obj":"Entity"},{"id":"T27868","span":{"begin":200,"end":203},"obj":"Protein"}],"relations":[{"id":"R19285","pred":"themeOf","subj":"T27871","obj":"T27877"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T27864","span":{"begin":144,"end":147},"obj":"Protein"},{"id":"T27863","span":{"begin":140,"end":143},"obj":"Protein"},{"id":"T27862","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

test3

{"project":"test3","denotations":[{"id":"T27773","span":{"begin":20,"end":28},"obj":"Protein"}],"text":"Stable Infection of Myr-Akt1\nTo generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs). Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene. Cells were selected and maintained in 10 µg/ml puromycin."}

PMC:3585731 / 56076-56510 JSONTXT

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PMC:3585731 / 56076-56510 JSON TXT