PMC:3585731 / 54812-54924 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T27181	82-111	NN	denotes	phenylmethanesulfonylfluoride
T27180	76-81	NN	denotes	µg/ml
T27179	73-75	CD	denotes	50
T27178	68-72	IN	denotes	with
T27177	55-67	VB	denotes	supplemented
T27176	53-54	-RRB-	denotes	)
T27175	44-53	NN	denotes	Signaling
T27174	39-43	NN	denotes	Cell
T27173	38-39	-LRB-	denotes	(
T27172	31-37	NN	denotes	buffer
T27171	24-30	NN	denotes	1×RIPA
T27170	21-23	IN	denotes	in
T27169	11-20	VB	denotes	harvested
T27168	6-10	VB	denotes	were
T27167	0-5	NN	denotes	Cells
R18665	T27167	T27168	arg1Of	Cells,were
R18666	T27167	T27169	arg2Of	Cells,harvested
R18667	T27169	T27168	arg2Of	harvested,were
R18668	T27169	T27170	arg1Of	harvested,in
R18669	T27172	T27170	arg2Of	buffer,in
R18670	T27172	T27171	arg1Of	buffer,1×RIPA
R18671	T27172	T27173	arg1Of	buffer,(
R18672	T27172	T27177	arg2Of	buffer,supplemented
R18673	T27175	T27173	arg2Of	Signaling,(
R18674	T27175	T27174	arg1Of	Signaling,Cell
R18675	T27176	T27173	arg3Of	),(
R18676	T27177	T27178	arg1Of	supplemented,with
R18677	T27181	T27178	arg2Of	phenylmethanesulfonylfluoride,with
R18678	T27181	T27179	arg1Of	phenylmethanesulfonylfluoride,50
R18679	T27181	T27180	arg1Of	phenylmethanesulfonylfluoride,µg/ml

Id	Subject	Object	Predicate	Lexical cue
T27067	0-112	Sentence	denotes	Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T345	0-112	Sentence	denotes	Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.

Id	Subject	Object	Predicate	Lexical cue
T27366	0-30	Binding	denotes	Cells were harvested in 1×RIPA
T27356	0-5	Entity	denotes	Cells
T27354	39-43	Entity	denotes	Cell

Id	Subject	Object	Predicate	Lexical cue
T27460	111-112	.	denotes	.
T27459	82-111	NN	denotes	phenylmethanesulfonylfluoride
T27458	79-81	NN	denotes	ml
T27457	78-79	NN	denotes	/
T27456	76-78	NN	denotes	µg
T27455	73-75	CD	denotes	50
T27454	68-72	IN	denotes	with
T27453	55-67	VBN	denotes	supplemented
T27452	53-54	-RRB-	denotes	)
T27451	44-53	NNP	denotes	Signaling
T27450	39-43	NNP	denotes	Cell
T27449	38-39	-LRB-	denotes	(
T27448	31-37	NN	denotes	buffer
T27447	26-30	NNP	denotes	RIPA
T27446	25-26	CD	denotes	×
T27445	24-25	CD	denotes	1
T27444	21-23	IN	denotes	in
T27443	11-20	VBN	denotes	harvested
T27442	6-10	VBD	denotes	were
T27441	0-5	NNS	denotes	Cells
R18893	T27441	T27443	nsubjpass	Cells,harvested
R18894	T27442	T27443	auxpass	were,harvested
R18895	T27443	T27443	ROOT	harvested,harvested
R18896	T27444	T27443	prep	in,harvested
R18897	T27445	T27446	nummod	1,×
R18898	T27446	T27448	nummod	×,buffer
R18899	T27447	T27448	compound	RIPA,buffer
R18900	T27448	T27444	pobj	buffer,in
R18901	T27449	T27448	punct	(,buffer
R18902	T27450	T27451	compound	Cell,Signaling
R18903	T27451	T27448	appos	Signaling,buffer
R18904	T27452	T27448	punct	),buffer
R18905	T27453	T27443	conj	supplemented,harvested
R18906	T27454	T27453	prep	with,supplemented
R18907	T27455	T27456	nummod	50,µg
R18908	T27456	T27459	nmod	µg,phenylmethanesulfonylfluoride
R18909	T27457	T27459	nmod	/,phenylmethanesulfonylfluoride
R18910	T27458	T27459	compound	ml,phenylmethanesulfonylfluoride
R18911	T27459	T27454	pobj	phenylmethanesulfonylfluoride,with
R18912	T27460	T27443	punct	.,harvested