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PMC:3585731 / 49870-57674 JSONTXT

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testone

Id Subject Object Predicate Lexical cue
T28164 7226-7230 Protein denotes TNFα
T28163 7209-7213 Protein denotes TNFα
T28276 7731-7746 Phosphorylation denotes Phosphorylation
T28275 7557-7564 Protein denotes Myr-Akt
T27772 6226-6234 Protein denotes Myr-Akt1
T27054 4936-4940 Protein denotes TNFα
T27053 4777-4781 Protein denotes TNFα
T26705 4532-4536 Protein denotes TNFα
T26704 4333-4338 Protein denotes Mapk9
T26703 4306-4311 Protein denotes Mapk8
T26702 4243-4247 Protein denotes PDK1
T26701 4191-4195 Protein denotes Akt3
T26700 4165-4169 Protein denotes Akt2
T26699 4139-4143 Protein denotes Akt1
T26184 2738-2747 Negative_regulation denotes deficient
T26183 2733-2737 Protein denotes FADD
T25976 2671-2674 Protein denotes 18S
T25975 2623-2626 Protein denotes 18S
T25974 2573-2577 Protein denotes TNFα
T25973 2522-2526 Protein denotes TNFα
T25972 2432-2435 Protein denotes 18S
T25971 2347-2351 Protein denotes TNFα
T25579 1331-1339 Protein denotes Myr-Akt1
T25578 1186-1199 Protein denotes Myr-Akt1-FLAG
T25577 1119-1127 Protein denotes Myr-Akt1
T25119 250-259 Negative_regulation denotes inhibitor
T25118 1012-1017 Protein denotes IGF-1
T25117 972-975 Protein denotes EGF
T25116 955-959 Protein denotes bFGF
T25115 932-936 Protein denotes TNFα
R17901 T26183 T26184 themeOf FADD,deficient

2_test

Id Subject Object Predicate Lexical cue
23469174-18408713-90505484 108-110 18408713 denotes 23
23469174-16153840-90505485 114-116 16153840 denotes 24
23469174-21041639-90505486 1181-1183 21041639 denotes 52
23469174-19825827-90505487 2867-2869 19825827 denotes 53

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T28361 7799-7803 NN denotes blot
T28360 7791-7798 JJ denotes western
T28359 7788-7790 IN denotes by
T28358 7777-7787 VB denotes visualized
T28357 7773-7776 VB denotes was
T28356 7765-7772 NN denotes protein
T28355 7758-7764 NN denotes fusion
T28354 7754-7757 NN denotes GSK
T28353 7750-7753 DT denotes the
T28352 7747-7749 IN denotes of
T28351 7731-7746 NN denotes Phosphorylation
T28350 7720-7729 NN denotes substrate
T28349 7712-7719 NN denotes protein
T28348 7705-7711 NN denotes fusion
T28347 7701-7704 NN denotes GSK
T28346 7699-7700 DT denotes a
T28345 7696-7698 IN denotes of
T28344 7687-7695 NN denotes presence
T28343 7683-7686 DT denotes the
T28342 7680-7682 IN denotes in
T28341 7670-7679 VB denotes performed
T28340 7666-7669 VB denotes was
T28339 7660-7665 NN denotes assay
T28216 7401-7405 NN denotes dish
T28215 7397-7400 NN denotes cm2
T28214 7394-7396 CD denotes 10
T28213 7392-7393 DT denotes a
T28212 7389-7391 IN denotes in
T28211 7381-7388 VB denotes treated
T28210 7377-7380 CC denotes and
T28209 7370-7376 VB denotes plated
T28208 7364-7369 NN denotes cells
T28207 7358-7363 CD denotes 3×106
T28206 7353-7357 IN denotes from
T28205 7344-7352 VB denotes prepared
T28204 7339-7343 VB denotes were
T28203 7331-7338 NN denotes lysates
T28202 7326-7330 NN denotes Cell
T28201 7312-7324 NN denotes descriptions
T28200 7297-7311 NN denotes manufacturer’s
T28199 7294-7296 TO denotes to
T28198 7284-7293 VB denotes according
T28197 7274-7283 VB denotes performed
T28196 7269-7273 VB denotes were
T28195 7267-7268 -RRB- denotes )
T28194 7260-7267 NNP denotes Systems
T28193 7258-7259 NNP denotes D
T28192 7257-7258 CC denotes &
T28191 7256-7257 NN denotes R
T28190 7255-7256 -LRB- denotes (
T28189 7248-7254 NN denotes assays
T28188 7242-7247 NN denotes ELISA
T28187 7231-7241 NN denotes Quantikine
T28186 7226-7230 NN denotes TNFα
T28185 7220-7225 NN denotes Mouse
T28184 7214-7219 NN denotes ELISA
T28183 7209-7213 NN denotes TNFα
T28058 7200-7206 NN denotes values
T28057 7192-7199 NN denotes pan-Akt
T28056 7189-7191 IN denotes on
T28055 7183-7188 VB denotes based
T28054 7172-7182 VB denotes normalized
T28053 7167-7171 VB denotes were
T28052 7148-7166 NN denotes phospho-antibodies
T28051 7144-7147 IN denotes for
T28050 7136-7143 NNP denotes Signals
T28049 7131-7135 NNP denotes 4°C.
T28048 7128-7130 IN denotes at
T28047 7118-7127 RB denotes overnight
T28046 7108-7117 VB denotes incubated
T28045 7104-7107 VB denotes was
T28044 7096-7103 NN denotes pan-Akt
T28043 7093-7095 TO denotes to
T28042 7084-7092 NN denotes antibody
T28041 7076-7083 JJ denotes Primary
T28040 7063-7074 NN denotes temperature
T28039 7058-7062 NN denotes room
T28038 7055-7057 IN denotes at
T28037 7052-7054 NN denotes hr
T28036 7050-7051 CD denotes 2
T28035 7046-7049 IN denotes for
T28034 7038-7045 NN denotes samples
T28033 7034-7037 DT denotes the
T28032 7029-7033 IN denotes with
T28031 7019-7028 VB denotes incubated
T28030 7014-7018 VB denotes were
T28029 6999-7013 NN denotes phopsho-Ser473
T28010 6881-6883 IN denotes of
T28009 6869-6880 NN denotes microliters
T28008 6864-6868 CD denotes Five
T28007 6857-6862 NN denotes blots
T28006 6849-6856 JJ denotes Western
T28005 6845-6848 IN denotes for
T28004 6835-6844 VB denotes described
T28003 6832-6834 IN denotes as
T28002 6825-6831 NN denotes buffer
T28001 6820-6824 NN denotes RIPA
T28000 6817-6819 IN denotes in
T27999 6808-6816 VB denotes prepared
T27998 6803-6807 VB denotes were
T27997 6795-6802 NN denotes lysates
T27996 6790-6794 NN denotes Cell
T27995 6775-6788 NN denotes modifications
T27994 6765-6774 VB denotes following
T27993 6761-6764 DT denotes the
T27992 6756-6760 IN denotes with
T27991 6747-6755 NN denotes protocol
T27990 6732-6746 NN denotes manufacturer’s
T27989 6729-6731 TO denotes to
T27988 6719-6728 VB denotes according
T27987 6709-6718 VB denotes performed
T27986 6704-6708 VB denotes were
T27985 6702-6703 -RRB- denotes )
T27984 6693-6702 NNP denotes Australia
T27983 6691-6692 -COMMA- denotes ,
T27982 6682-6691 NNP denotes Hindmarsh
T27981 6680-6681 -COMMA- denotes ,
T27980 6674-6680 NNP denotes TGRBio
T27979 6673-6674 -LRB- denotes (
T27978 6666-6672 NN denotes assays
T27977 6657-6665 NN denotes ELISAOne
T27976 6651-6656 NN denotes Assay
T27857 6630-6639 NN denotes puromycin
T27856 6624-6629 NN denotes µg/ml
T27855 6621-6623 CD denotes 10
T27854 6618-6620 IN denotes in
T27853 6607-6617 VB denotes maintained
T27852 6603-6606 CC denotes and
T27851 6594-6602 VB denotes selected
T27850 6589-6593 VB denotes were
T27849 6583-6588 NN denotes Cells
T27848 6572-6581 NN denotes polybrene
T27847 6566-6571 NN denotes µg/ml
T27846 6564-6565 CD denotes 8
T27845 6559-6563 IN denotes with
T27844 6553-6558 NN denotes cells
T27843 6548-6552 NN denotes L929
T27842 6545-6547 TO denotes to
T27841 6537-6544 VB denotes applied
T27840 6533-6536 CC denotes and
T27839 6526-6532 NN denotes filter
T27838 6523-6525 NN denotes µm
T27837 6518-6522 CD denotes 0.45
T27836 6510-6517 IN denotes through
T27835 6501-6509 VB denotes filtered
T27834 6499-6500 -COMMA- denotes ,
T27833 6494-6499 RB denotes later
T27832 6491-6493 NN denotes hr
T27831 6488-6490 CD denotes 72
T27830 6478-6487 VB denotes collected
T27829 6474-6477 VB denotes was
T27828 6468-6473 NN denotes media
T27827 6451-6467 JJ denotes Virus-containing
T27826 6448-6449 -RRB- denotes )
T27825 6444-6448 NN denotes Labs
T27824 6435-6443 NN denotes Signagen
T27823 6434-6435 -LRB- denotes (
T27822 6426-6433 NN denotes reagent
T27821 6413-6425 NN denotes transfection
T28338 7654-7659 FW denotes vitro
T28337 7651-7653 FW denotes in
T28336 7647-7650 DT denotes The
T28335 7644-7645 -RRB- denotes )
T28334 7639-7644 NNP denotes Sigma
T28333 7638-7639 -LRB- denotes (
T28332 7632-7637 NN denotes beads
T28331 7623-7631 JJ denotes magnetic
T28330 7620-7622 NN denotes M2
T28329 7610-7619 JJ denotes anti-FLAG
T28328 7604-7609 VB denotes using
T28327 7598-7603 NN denotes cells
T28326 7593-7597 NN denotes L929
T28325 7588-7592 IN denotes from
T28324 7569-7587 VB denotes immunoprecipitated
T28323 7565-7568 VB denotes was
T28322 7557-7564 NN denotes Myr-Akt
T28321 7555-7556 -COMMA- denotes ,
T28320 7550-7555 NN denotes brief
T28319 7547-7549 IN denotes In
T28318 7535-7546 NNP denotes Technology.
T28317 7525-7534 NNP denotes Signaling
T28316 7520-7524 NNP denotes Cell
T28315 7515-7519 IN denotes from
T28314 7513-7514 -RRB- denotes )
T28313 7499-7513 JJ denotes nonradioactive
T28312 7498-7499 -LRB- denotes (
T28311 7494-7497 NN denotes kit
T28310 7488-7493 NN denotes assay
T28309 7481-7487 NN denotes kinase
T28308 7477-7480 NN denotes Akt
T28307 7473-7476 DT denotes the
T28306 7467-7472 VB denotes using
T28305 7458-7466 VB denotes measured
T28304 7454-7457 VB denotes was
T28303 7445-7453 NN denotes activity
T28302 7438-7444 NN denotes kinase
T28301 7434-7437 NN denotes Akt
T28300 7428-7433 NNP denotes Assay
T28299 7421-7427 NNP denotes Kinase
T28298 7417-7420 NNP denotes Akt
T28297 7411-7416 FW denotes vitro
T28296 7408-7410 FW denotes In
T27820 6406-6412 NN denotes GenJet
T27819 6400-6405 VB denotes using
T27818 6393-6399 VB denotes plates
T27817 6388-6392 RB denotes well
T27816 6386-6387 CD denotes 6
T27815 6383-6385 IN denotes in
T27814 6374-6382 NN denotes plasmids
T27813 6364-6373 JJ denotes accessory
T27812 6358-6363 JJ denotes VSV-G
T27811 6354-6357 CC denotes and
T27810 6346-6353 NN denotes gal/pol
T27809 6343-6345 IN denotes of
T27808 6340-6342 NN denotes µg
T27807 6338-6339 CD denotes 1
T27806 6334-6337 CC denotes and
T27805 6330-6333 NN denotes DNA
T27804 6324-6329 JJ denotes viral
T27803 6321-6323 IN denotes of
T27802 6318-6320 NN denotes µg
T27801 6316-6317 CD denotes 2
T27800 6311-6315 IN denotes with
T27799 6299-6310 VB denotes transfected
T27798 6294-6298 VB denotes were
T27797 6292-6293 -RRB- denotes )
T27796 6282-6292 NN denotes Invitrogen
T27795 6281-6282 -LRB- denotes (
T27675 6137-6146 NN denotes SYBRGreen
T27674 6131-6136 VB denotes using
T27673 6122-6130 NN denotes performs
T27672 6117-6121 VB denotes were
T27671 6107-6116 NN denotes Reactions
T27670 6096-6105 NN denotes reactions
T27669 6091-6095 NN denotes qPCR
T27668 6088-6090 IN denotes in
T27667 6080-6087 NN denotes primers
T27666 6077-6079 NN denotes pM
T27665 6073-6076 CD denotes 500
T27664 6068-6072 IN denotes with
T27663 6063-6067 VB denotes used
T27662 6059-6062 VB denotes was
T27661 6054-6058 NN denotes cDNA
T27660 6051-6053 IN denotes of
T27659 6048-6050 NN denotes µL
T27658 6046-6047 CD denotes 1
T27657 6043-6044 -RRB- denotes )
T27656 6036-6043 NNP denotes Biolabs
T27655 6028-6035 NNP denotes England
T27654 6024-6027 NNP denotes New
T27653 6022-6023 -COMMA- denotes ,
T27652 6019-6022 NN denotes kit
T27651 6014-6018 NN denotes cDNA
T27650 6007-6013 NN denotes M-MuLV
T27649 6006-6007 -LRB- denotes (
T27648 5998-6005 NN denotes primers
T27647 5991-5997 JJ denotes random
T27646 5985-5990 VB denotes using
T27645 5980-5984 NN denotes cDNA
T27644 5977-5979 TO denotes to
T27643 5967-5976 VB denotes converted
T27642 5963-5966 VB denotes was
T27641 5959-5962 NN denotes RNA
T27640 5956-5958 IN denotes of
T27639 5953-5955 NN denotes µg
T27638 5951-5952 CD denotes 1
T27637 5948-5949 -RRB- denotes )
T27636 5940-5948 NNP denotes Research
T27635 5935-5939 NNP denotes Zymo
T27634 5934-5935 -LRB- denotes (
T27633 5930-5933 NN denotes kit
T27632 5921-5929 NN denotes Miniprep
T27631 5918-5920 NN denotes ZR
T27630 5912-5917 VB denotes using
T27629 5903-5911 VB denotes isolated
T27628 5899-5902 VB denotes was
T27627 5895-5898 NN denotes RNA
T27626 5889-5894 JJ denotes Total
T27625 5882-5887 NN denotes blots
T27624 5874-5881 JJ denotes Western
T27623 5870-5873 IN denotes for
T27622 5860-5869 VB denotes described
T27621 5857-5859 IN denotes as
T27620 5849-5856 VB denotes treated
T27619 5844-5848 VB denotes were
T27618 5838-5843 NN denotes Cells
T27617 5830-5837 NN denotes qRT-PCR
T28028 6995-6998 CC denotes and
T28027 6980-6994 NN denotes phopsho-Thr308
T28026 6977-6979 TO denotes to
T28025 6966-6976 NN denotes antibodies
T28024 6958-6965 JJ denotes Primary
T28023 6948-6956 NN denotes analysis
T28022 6945-6947 TO denotes to
T28021 6939-6944 JJ denotes prior
T28020 6932-6938 NN denotes buffer
T28019 6926-6931 NN denotes lysis
T28018 6917-6925 NN denotes ELISAOne
T28017 6914-6916 IN denotes of
T28016 6911-6913 NN denotes µL
T28015 6908-6910 CD denotes 45
T28014 6905-6907 IN denotes in
T28013 6897-6904 VB denotes diluted
T28012 6892-6896 VB denotes were
T28011 6884-6891 NN denotes samples
T27316 5817-5827 NN denotes antibodies
T27315 5813-5816 JJ denotes new
T27314 5808-5812 IN denotes with
T27313 5799-5807 VB denotes reprobed
T27312 5795-5798 CC denotes and
T27311 5793-5794 -RRB- denotes )
T27310 5782-5793 NNP denotes Biosciences
T27309 5779-5781 NNP denotes GM
T27308 5778-5779 -LRB- denotes (
T27307 5771-5777 NN denotes buffer
T27306 5761-5770 VB denotes stripping
T27305 5751-5760 NN denotes OneMinute
T27304 5745-5750 VB denotes using
T27303 5736-5744 VB denotes stripped
T27302 5731-5735 VB denotes were
T27301 5721-5730 NN denotes membranes
T27300 5719-5720 -COMMA- denotes ,
T27299 5714-5719 NN denotes cases
T27298 5709-5713 DT denotes some
T27297 5706-5708 IN denotes In
T27296 5697-5704 NN denotes signals
T27295 5693-5696 DT denotes the
T27294 5685-5692 VB denotes develop
T27293 5682-5684 TO denotes to
T27292 5677-5681 VB denotes used
T27291 5672-5676 VB denotes were
T27290 5663-5671 NN denotes reagents
T27289 5659-5662 NN denotes ECL
T27288 5657-5658 -RRB- denotes )
T27287 5648-5657 NNP denotes Millipore
T27286 5647-5648 -LRB- denotes (
T27285 5638-5646 NNP denotes Luminata
T27284 5625-5636 NN denotes temperature
T27283 5620-5624 NN denotes room
T27282 5617-5619 IN denotes at
T27281 5613-5616 NN denotes min
T27280 5610-5612 CD denotes 30
T27279 5606-5609 IN denotes for
T27278 5601-5605 NN denotes TBST
T27277 5598-5600 IN denotes in
T27276 5588-5597 VB denotes incubated
T27275 5583-5587 VB denotes were
T27274 5572-5582 NN denotes antibodies
T27273 5562-5571 NNP denotes Secondary
T27272 5557-5561 NNP denotes 4°C.
T27271 5554-5556 IN denotes at
T27270 5544-5553 RB denotes overnight
T27269 5535-5543 NN denotes BSA/TBST
T27268 5534-5535 NN denotes %
T27267 5533-5534 CD denotes 5
T27266 5530-5532 IN denotes in
T27265 5520-5529 VB denotes incubated
T27264 5515-5519 VB denotes were
T27263 5504-5514 NN denotes antibodies
T27262 5496-5503 JJ denotes Primary
T27261 5483-5494 NN denotes temperature
T27260 5478-5482 NN denotes room
T27259 5475-5477 IN denotes at
T27258 5471-5474 NN denotes min
T27257 5468-5470 CD denotes 30
T27256 5464-5467 IN denotes for
T27255 5457-5463 NN denotes buffer
T27254 5452-5456 NN denotes TBST
T27253 5449-5451 IN denotes in
T27252 5447-5448 -RRB- denotes )
T27251 5444-5447 NN denotes BSA
T27250 5443-5444 -LRB- denotes (
T27249 5435-5442 NN denotes albumin
T27248 5429-5434 NN denotes serum
T27247 5422-5428 JJ denotes bovine
T27246 5420-5421 NN denotes %
T27245 5419-5420 CD denotes 5
T27244 5416-5418 CC denotes or
T27243 5411-5415 NN denotes milk
T27242 5409-5410 NN denotes %
T27241 5408-5409 CD denotes 3
T27240 5405-5407 IN denotes in
T27239 5397-5404 VB denotes blocked
T27238 5395-5396 -COMMA- denotes ,
T27237 5387-5395 NN denotes membrane
T27236 5382-5386 NN denotes PVDF
T27235 5379-5381 TO denotes to
T27234 5367-5378 VB denotes transferred
T27233 5362-5366 VB denotes were
T27232 5357-5361 NN denotes gels
T27231 5348-5356 NN denotes SDS-PAGE
T27230 5346-5347 -COMMA- denotes ,
T27229 5339-5346 RB denotes Briefly
T27228 5328-5337 NN denotes protocols
T27227 5319-5327 JJ denotes standard
T27226 5316-5318 TO denotes to
T27225 5306-5315 VB denotes according
T27224 5296-5305 VB denotes performed
T27223 5292-5295 VB denotes was
T27222 5283-5291 NN denotes blotting
T27221 5275-5282 NNP denotes Western
T27220 5269-5274 NNP denotes 95°C.
T27219 5266-5268 IN denotes at
T27218 5262-5265 NN denotes min
T27217 5260-5261 CD denotes 5
T27216 5256-5259 IN denotes for
T27215 5249-5255 VB denotes boiled
T27214 5244-5248 VB denotes were
T27213 5235-5243 NN denotes proteins
T27212 5232-5234 IN denotes of
T27211 5224-5231 NN denotes amounts
T27210 5218-5223 JJ denotes Equal
T27209 5215-5216 -RRB- denotes )
T27208 5209-5215 NNP denotes Pierce
T27207 5208-5209 -LRB- denotes (
T27206 5200-5207 NN denotes Reagent
T27205 5194-5199 NN denotes Assay
T27204 5191-5193 NN denotes nm
T27203 5187-5190 CD denotes 660
T27202 5180-5186 NNP denotes Pierce
T27201 5176-5179 DT denotes the
T27200 5170-5175 VB denotes using
T27199 5161-5169 VB denotes measured
T27198 5156-5160 VB denotes were
T27197 5141-5155 NN denotes concentrations
T27196 5133-5140 NN denotes Protein
T27195 5121-5131 NN denotes 14,000×rpm
T27194 5118-5120 IN denotes at
T27193 5114-5117 NN denotes min
T27192 5111-5113 CD denotes 15
T27191 5107-5110 IN denotes for
T27190 5102-5106 RP denotes down
T27189 5097-5101 VB denotes spun
T27188 5092-5096 VB denotes were
T27187 5084-5091 NN denotes lysates
T27186 5079-5083 NN denotes cell
T27185 5077-5078 -COMMA- denotes ,
T27184 5067-5077 NN denotes sonication
T27183 5061-5066 JJ denotes brief
T27182 5055-5060 IN denotes After
T27181 5024-5053 NN denotes phenylmethanesulfonylfluoride
T27180 5018-5023 NN denotes µg/ml
T27179 5015-5017 CD denotes 50
T27178 5010-5014 IN denotes with
T27177 4997-5009 VB denotes supplemented
T27176 4995-4996 -RRB- denotes )
T27175 4986-4995 NN denotes Signaling
T27174 4981-4985 NN denotes Cell
T27173 4980-4981 -LRB- denotes (
T27172 4973-4979 NN denotes buffer
T27171 4966-4972 NN denotes 1×RIPA
T27170 4963-4965 IN denotes in
T27169 4953-4962 VB denotes harvested
T27168 4948-4952 VB denotes were
T27167 4942-4947 NN denotes Cells
T27166 4936-4940 NN denotes TNFα
T27165 4930-4935 NN denotes mouse
T27164 4924-4929 NN denotes ng/ml
T27163 4921-4923 CD denotes 10
T27162 4918-4920 CC denotes or
T27161 4909-4917 NN denotes zVAD.fmk
T27160 4906-4908 NN denotes µM
T27159 4903-4905 CD denotes 20
T27158 4901-4902 -COMMA- denotes ,
T27157 4894-4901 NN denotes factors
T27156 4887-4893 NN denotes growth
T27794 6275-6280 NN denotes cells
T27793 6266-6274 NN denotes HEK293FT
T27792 6264-6265 -COMMA- denotes ,
T27791 6252-6264 NN denotes retroviruses
T27790 6247-6251 NN denotes MSCV
T27789 6238-6246 VB denotes generate
T27788 6235-6237 TO denotes To
T27787 6226-6234 NN denotes Myr-Akt1
T27786 6223-6225 IN denotes of
T27785 6213-6222 NN denotes Infection
T27784 6206-6212 JJ denotes Stable
T27686 6202-6203 -RRB- denotes )
T27685 6197-6202 NN denotes Roche
T27684 6196-6197 -LRB- denotes (
T27683 6181-6195 NN denotes LightCycler480
T27682 6179-6180 DT denotes a
T27681 6176-6178 IN denotes in
T27680 6174-6175 -RRB- denotes )
T27679 6161-6174 NN denotes SABiosciences
T27678 6160-6161 -LRB- denotes (
T27677 6156-6159 NN denotes mix
T27676 6147-6155 NN denotes 2×Master
T27155 4884-4886 IN denotes of
T27154 4875-4883 NN denotes addition
T27153 4871-4874 DT denotes the
T27152 4868-4870 TO denotes to
T27151 4862-4867 JJ denotes prior
T27150 4859-4861 NN denotes hr
T27149 4856-4858 CD denotes 24
T27148 4852-4855 IN denotes for
T27147 4844-4851 VB denotes starved
T27146 4838-4843 NN denotes serum
T27145 4833-4837 VB denotes were
T27144 4827-4832 NN denotes cells
T27143 4825-4826 -COMMA- denotes ,
T27142 4815-4825 NN denotes conditions
T27141 4810-4814 JJ denotes free
T27140 4804-4809 NN denotes serum
T27139 4798-4803 IN denotes under
T27138 4787-4797 NN denotes treatments
T27137 4783-4786 IN denotes For
T27136 4777-4781 NN denotes TNFα
T27135 4771-4776 NN denotes mouse
T27134 4765-4770 NN denotes ng/ml
T27133 4762-4764 CD denotes 10
T27132 4759-4761 CC denotes or
T27131 4750-4758 NN denotes zVAD.fmk
T27130 4747-4749 NN denotes µM
T27123 4713-4715 NN denotes hr
T27122 4707-4712 CD denotes 24–48
T27121 4701-4706 IN denotes After
T27120 4693-4699 NN denotes dishes
T27119 4689-4692 NN denotes mm2
T27118 4686-4688 CD denotes 35
T27117 4681-4685 IN denotes into
T27116 4674-4680 VB denotes seeded
T27115 4669-4673 VB denotes were
T27114 4667-4668 -RRB- denotes )
T27113 4662-4667 NN denotes cells
T27112 4655-4661 NN denotes Jurkat
T27111 4649-4654 CD denotes 1×106
T27110 4648-4649 -LRB- denotes (
T27109 4642-4647 NN denotes cells
T27108 4633-4641 JJ denotes adherent
T27107 4627-4632 CD denotes 4×105
T27106 4625-4626 -COMMA- denotes ,
T27105 4621-4625 NN denotes blot
T27104 4613-4620 JJ denotes Western
T27103 4609-4612 IN denotes For
T27102 4604-4608 NNP denotes Blot
T27101 4596-4603 NNP denotes Western
T26858 4592-4593 -RRB- denotes )
T26857 4583-4592 NN denotes viability
T26856 4578-4582 NN denotes cell
T26855 4577-4578 -LRB- denotes (
T26854 4574-4576 NN denotes hr
T26853 4571-4573 CD denotes 24
T26852 4568-4570 CC denotes or
T26851 4566-4567 -RRB- denotes )
T26850 4562-4566 NN denotes blot
T26849 4554-4561 JJ denotes Western
T26848 4551-4553 CC denotes or
T26847 4547-4550 NN denotes RNA
T26846 4546-4547 -LRB- denotes (
T26845 4543-4545 NN denotes hr
T26844 4541-4542 CD denotes 9
T26843 4537-4540 IN denotes for
T26842 4532-4536 NN denotes TNFα
T26841 4529-4531 CC denotes or
T26840 4520-4528 NN denotes zVAD.fmk
T26839 4515-4519 IN denotes with
T26838 4507-4514 VB denotes treated
T26837 4502-4506 VB denotes were
T26836 4496-4501 NN denotes cells
T26835 4494-4495 -COMMA- denotes ,
T26834 4492-4494 NN denotes hr
T26833 4489-4491 CD denotes 72
T26832 4483-4488 IN denotes After
T26831 4454-4469 NN denotes ufacturer’s rec
T26830 4440-4454 NN denotes cording to man
T26829 4438-4440 TO denotes ac
T26828 4429-4438 VB denotes trogen),
T26827 4428-4429 -COMMA- denotes i
T26826 4427-4428 -RRB- denotes v
T26825 4417-4427 NN denotes eagent (In
T26824 4416-4417 -LRB- denotes r
T26823 4409-4416 NN denotes NAiMAX
T26822 4402-4409 NN denotes using R
T26821 4397-4402 VB denotes cted
T26820 4386-4397 VB denotes ere transfe
T26819 4382-4386 VB denotes NA w
T26818 4377-4382 NN denotes . siR
T26817 4376-4377 -RRB- denotes )
T26816 4365-4376 NN denotes L-043776-00
T26815 4364-4365 -LRB- denotes (
T26814 4360-4363 NN denotes Jun
T26813 4354-4359 NN denotes mouse
T26812 4352-4353 -COMMA- denotes ,
T26811 4351-4352 -RRB- denotes )
T26810 4340-4351 NN denotes J-040134-05
T26809 4339-4340 -LRB- denotes (
T26808 4333-4338 NN denotes Mapk9
T26807 4327-4332 NN denotes mouse
T26806 4325-4326 -COMMA- denotes ,
T26805 4324-4325 -RRB- denotes )
T26804 4313-4324 NN denotes J-040128-05
T26803 4312-4313 -LRB- denotes (
T26802 4306-4311 NN denotes Mapk8
T26801 4300-4305 NN denotes mouse
T26800 4298-4299 -COMMA- denotes ,
T26799 4297-4298 -RRB- denotes )
T26798 4283-4297 NN denotes D-001810-10-05
T26797 4282-4283 -LRB- denotes (
T26796 4274-4281 NN denotes control
T26795 4263-4273 JJ denotes non-coding
T26794 4261-4262 -COMMA- denotes ,
T26793 4260-4261 -RRB- denotes )
T26792 4249-4260 NN denotes L-040658-00
T26791 4248-4249 -LRB- denotes (
T26790 4243-4247 NN denotes PDK1
T26789 4237-4242 NN denotes mouse
T26788 4235-4236 -COMMA- denotes ,
T26787 4234-4235 -RRB- denotes )
T26786 4223-4234 NN denotes L-065427-00
T26785 4222-4223 -LRB- denotes (
T26784 4217-4221 NN denotes mTOR
T26783 4211-4216 NN denotes mouse
T26782 4209-4210 -COMMA- denotes ,
T26781 4208-4209 -RRB- denotes )
T26780 4197-4208 NN denotes L-040891-00
T26779 4196-4197 -LRB- denotes (
T26778 4191-4195 NN denotes Akt3
T26777 4185-4190 NN denotes mouse
T26776 4183-4184 -COMMA- denotes ,
T26775 4182-4183 -RRB- denotes )
T26774 4171-4182 NN denotes L-040782-00
T26773 4170-4171 -LRB- denotes (
T26772 4165-4169 NN denotes Akt2
T26771 4159-4164 NN denotes mouse
T26770 4157-4158 -COMMA- denotes ,
T26769 4156-4157 -RRB- denotes )
T26768 4145-4156 NN denotes L-040709-00
T26767 4144-4145 -LRB- denotes (
T26766 4139-4143 NN denotes Akt1
T26765 4133-4138 NN denotes mouse
T26764 4131-4132 -COMMA- denotes ,
T26763 4130-4131 -RRB- denotes )
T26762 4119-4130 NN denotes L-045791-00
T26761 4115-4118 CC denotes and
T26760 4103-4114 NN denotes L-040893-00
T26759 4102-4103 -LRB- denotes (
T26758 4094-4101 NN denotes protein
T26757 4091-4093 NN denotes S6
T26756 4081-4090 JJ denotes ribosomal
T26755 4075-4080 NNP denotes Mouse
T26754 4064-4074 NNP denotes Dharmacon.
T26753 4059-4063 IN denotes from
T26752 4049-4058 VB denotes purchased
T26751 4044-4048 VB denotes were
T26750 4037-4043 NN denotes siRNAs
T26749 4027-4036 NN denotes Knockdown
T26748 4021-4026 NN denotes siRNA
T27129 4744-4746 CD denotes 30
T27128 4739-4743 IN denotes with
T27127 4728-4738 VB denotes stimulated
T27126 4723-4727 VB denotes were
T27125 4717-4722 NN denotes cells
T27124 4715-4716 -COMMA- denotes ,
T26061 2684-2710 NN denotes 5′-TAGTAGCGACGGGCGGTGTG-3′
T26060 2676-2683 JJ denotes reverse
T26059 2674-2675 -COLON- denotes :
T26058 2671-2674 NN denotes 18S
T26057 2665-2670 JJ denotes human
T26056 2663-2664 -COMMA- denotes ,
T26055 2661-2663 CD denotes -3
T26054 2640-2660 NN denotes CAGCCACCCGAGATTGAGCA
T26053 2636-2639 CD denotes 5′-
T26052 2628-2635 RB denotes forward
T26051 2626-2627 -COLON- denotes :
T26050 2623-2626 NN denotes 18S
T26049 2617-2622 JJ denotes human
T26048 2615-2616 -COLON- denotes ;
T26047 2587-2615 NN denotes 5′-GAGGGCTGATTAGAGAGAGGTC-3′
T26046 2579-2586 JJ denotes reverse
T26045 2577-2578 -COLON- denotes :
T26044 2573-2577 NN denotes TNFα
T26043 2567-2572 JJ denotes human
T26042 2565-2566 -COMMA- denotes ,
T26041 2540-2565 NN denotes ATGAGCACTGAAAGCATGATCC-3′
T26040 2536-2539 CD denotes 5′-
T26039 2528-2535 RB denotes forward
T26038 2526-2527 -COLON- denotes :
T26037 2522-2526 NN denotes TNFα
T26036 2516-2521 JJ denotes human
T26035 2515-2516 -COLON- denotes ;
T26034 2485-2515 NN denotes 5′-CTAAACCATCCAATCGGTAGTAGC-3′
T26033 2477-2484 JJ denotes reverse
T26032 2475-2476 -COMMA- denotes ,
T26031 2449-2475 NN denotes ATAACAGGTCTGTGATGCCCTTAG-3
T26030 2445-2448 JJ denotes 5-′
T26029 2437-2444 RB denotes forward
T26028 2435-2436 -COLON- denotes :
T26027 2432-2435 NN denotes 18S
T26026 2426-2431 NN denotes mouse
T26025 2425-2426 -COLON- denotes ;
T26024 2400-2425 NN denotes 5′-GCTACGACGTGGGCTACAG-3′
T26023 2392-2399 JJ denotes reverse
T26022 2390-2391 -COMMA- denotes ,
T26021 2361-2390 JJ denotes 5′-CCCTCACACTCAGATCATCTTCT-3′
T26020 2353-2360 RB denotes forward
T26019 2351-2352 -COLON- denotes :
T26018 2347-2351 NN denotes TNFα
T26017 2341-2346 NN denotes Mouse
T26016 2333-2340 NN denotes Primers
T26015 2328-2332 NN denotes QPCR
T25741 1381-1391 NN denotes Antibodies
T26582 4009-4018 NN denotes molecules
T26581 4003-4008 JJ denotes small
T26580 3999-4002 DT denotes the
T26579 3996-3998 IN denotes of
T26578 3987-3995 NN denotes toxicity
T26577 3974-3986 JJ denotes non-specific
T26576 3971-3973 IN denotes of
T26575 3963-3970 NN denotes effects
T26574 3954-3962 JJ denotes possible
T26573 3950-3953 DT denotes the
T26572 3942-3949 VB denotes exclude
T26571 3939-3941 TO denotes to
T26570 3931-3938 VB denotes plotted
T26569 3927-3930 CC denotes and
T26568 3916-3926 VB denotes determined
T26567 3912-3915 VB denotes was
T26566 3910-3911 -COMMA- denotes ,
T26565 3905-3910 NN denotes cells
T26564 3888-3904 JJ denotes compound-treated
T26563 3880-3887 NN denotes control
T26562 3876-3879 DT denotes the
T26561 3873-3875 TO denotes to
T26560 3864-3872 JJ denotes relative
T26559 3855-3863 NN denotes compound
T26558 3851-3854 DT denotes the
T26557 3846-3850 IN denotes with
T26556 3838-3845 VB denotes treated
T26555 3834-3837 CC denotes and
T26554 3822-3833 NN denotes necroptosis
T26553 3814-3821 VB denotes undergo
T26552 3811-3813 TO denotes to
T26551 3803-3810 VB denotes induced
T26550 3801-3802 -COMMA- denotes ,
T26549 3796-3801 NN denotes cells
T26548 3793-3795 IN denotes of
T26547 3783-3792 NN denotes viability
T26546 3774-3782 JJ denotes Relative
T26545 3771-3772 NN denotes %
T26544 3768-3771 CD denotes 100
T26543 3765-3767 IN denotes as
T26542 3761-3764 VB denotes set
T26541 3757-3760 VB denotes was
T26540 3751-3756 NN denotes cells
T26539 3741-3750 JJ denotes untreated
T26538 3733-3740 NN denotes control
T26537 3729-3732 DT denotes the
T26536 3726-3728 IN denotes of
T26535 3716-3725 NN denotes Viability
T26534 3704-3714 VB denotes triplicate
T26533 3701-3703 CC denotes or
T26532 3691-3700 NN denotes duplicate
T26531 3688-3690 IN denotes in
T26530 3678-3687 VB denotes performed
T26529 3673-3677 VB denotes were
T26528 3661-3672 NN denotes Experiments
T26527 3658-3659 -RRB- denotes )
T26526 3651-3658 NNP denotes Promega
T26525 3650-3651 -LRB- denotes (
T26524 3644-3649 NNP denotes Assay
T26523 3634-3643 NNP denotes Viability
T26522 3629-3633 NNP denotes Cell
T26521 3615-3628 NNP denotes CellTiter-Glo
T26520 3609-3614 VB denotes using
T26519 3598-3608 VB denotes determined
T26518 3594-3597 VB denotes was
T26517 3584-3593 NN denotes viability
T26516 3579-3583 NN denotes Cell
T26515 3566-3577 NN denotes experiments
T26514 3561-3565 NN denotes blot
T26513 3553-3560 JJ denotes western
T26512 3549-3552 IN denotes for
T26511 3539-3548 VB denotes described
T26510 3536-3538 IN denotes as
T26509 3528-3535 VB denotes treated
T26508 3524-3527 CC denotes and
T26507 3513-3523 NN denotes cells/well
T26506 3507-3512 CD denotes 1×104
T26505 3504-3506 IN denotes of
T26504 3496-3503 NN denotes density
T26503 3492-3495 DT denotes the
T26502 3489-3491 IN denotes at
T26501 3482-3488 VB denotes plates
T26500 3477-3481 RB denotes well
T26499 3474-3476 CD denotes 96
T26498 3467-3473 NN denotes bottom
T26497 3461-3466 JJ denotes clear
T26496 3455-3460 JJ denotes white
T26495 3450-3454 IN denotes into
T26494 3443-3449 VB denotes seeded
T26493 3438-3442 VB denotes were
T26492 3432-3437 NN denotes Cells
T26491 3420-3431 NN denotes Experiments
T26490 3410-3419 NN denotes Viability
T26489 3405-3409 NN denotes Cell
T26325 3380-3402 JJ denotes antibiotic-antimycotic
T26324 3378-3379 NN denotes %
T26323 3377-3378 CD denotes 1
T26322 3373-3376 CC denotes and
T26321 3371-3372 -RRB- denotes )
T26320 3365-3371 NNP denotes Gemini
T26319 3364-3365 -LRB- denotes (
T26318 3354-3363 NN denotes FetalPlex
T26317 3352-3353 NN denotes %
T26316 3350-3352 CD denotes 10
T26315 3345-3349 IN denotes with
T26314 3332-3344 VB denotes supplemented
T26313 3330-3331 -COMMA- denotes ,
T26312 3322-3330 NN denotes RPMI1640
T26311 3319-3321 IN denotes in
T26310 3308-3318 VB denotes maintained
T26309 3303-3307 VB denotes were
T26308 3297-3302 NN denotes cells
T26307 3290-3296 NN denotes Jurkat
T26306 3280-3288 NN denotes pyruvate
T26305 3273-3279 NN denotes sodium
T26304 3269-3272 CC denotes and
T26303 3267-3268 -COMMA- denotes ,
T26302 3262-3267 NN denotes acids
T26301 3256-3261 NN denotes amino
T26300 3242-3255 JJ denotes non-essential
T26299 3240-3241 -COMMA- denotes ,
T26298 3229-3240 NN denotes L-glutamine
T26297 3224-3228 IN denotes with
T26296 3211-3223 VB denotes supplemented
T26295 3198-3210 RB denotes additionally
T26294 3194-3197 VB denotes was
T26293 3188-3193 NN denotes media
T26292 3177-3187 NN denotes fibroblast
T26291 3172-3176 NN denotes lung
T26290 3166-3171 NN denotes mouse
T26289 3162-3165 DT denotes The
T26288 3159-3160 -RRB- denotes )
T26287 3149-3159 NN denotes Invitrogen
T26286 3148-3149 -LRB- denotes (
T26285 3140-3147 NN denotes mixture
T26284 3117-3139 JJ denotes antibiotic-antimycotic
T26283 3115-3116 NN denotes %
T26282 3114-3115 CD denotes 1
T26281 3110-3113 CC denotes and
T26280 3108-3109 -RRB- denotes )
T26279 3105-3108 NN denotes FBS
T26278 3104-3105 -LRB- denotes (
T26277 3098-3103 NN denotes serum
T26276 3091-3097 JJ denotes bovine
T26275 3085-3090 JJ denotes fetal
T26274 3083-3084 NN denotes %
T26273 3081-3083 CD denotes 10
T26272 3076-3080 IN denotes with
T26271 3063-3075 VB denotes supplemented
T26270 3058-3062 NN denotes DMEM
T26269 3055-3057 IN denotes in
T26268 3044-3054 VB denotes maintained
T26267 3039-3043 VB denotes were
T26266 3033-3038 NN denotes Cells
T26265 3019-3031 RB denotes respectively
T26264 3017-3018 -COMMA- denotes ,
T26263 3016-3017 -RRB- denotes )
T26262 3006-3016 NNP denotes University
T26261 3000-3005 NNP denotes Tufts
T26260 2999-3000 -LRB- denotes (
T26259 2990-2998 NNP denotes Poltorak
T26258 2980-2989 NNP denotes Alexander
T26257 2976-2979 CC denotes and
T26256 2974-2975 -RRB- denotes )
T26255 2964-2974 NNP denotes University
T26254 2956-2963 NNP denotes Harvard
T26253 2955-2956 -LRB- denotes (
T26252 2950-2954 NNP denotes Yuan
T26251 2942-2949 NNP denotes Junying
T26250 2939-2941 IN denotes of
T26249 2933-2938 NN denotes gifts
T26248 2924-2932 JJ denotes generous
T26247 2919-2923 VB denotes were
T26246 2913-2918 NN denotes cells
T26245 2911-2912 -RRB- denotes )
T26244 2907-2911 NN denotes ATCC
T26243 2906-2907 -LRB- denotes (
T26242 2897-2905 NN denotes RAW264.7
T26241 2893-2896 CC denotes and
T26240 2887-2892 NN denotes cells
T26239 2885-2886 -RRB- denotes )
T26238 2881-2885 NN denotes ATCC
T26237 2880-2881 -LRB- denotes (
T26236 2872-2879 NN denotes J774A.1
T26235 2869-2870 -RRB- denotes ]
T26234 2867-2869 CD denotes 53
T26233 2866-2867 -LRB- denotes [
T26232 2864-2865 -RRB- denotes )
T26231 2854-2864 NNP denotes University
T26230 2848-2853 NNP denotes Tufts
T26229 2847-2848 -LRB- denotes (
T26228 2838-2846 NNP denotes Tsichlis
T26227 2831-2837 NNP denotes Philip
T26226 2827-2830 NNP denotes Dr.
T26225 2824-2826 IN denotes of
T26224 2819-2823 NN denotes gift
T26223 2810-2818 JJ denotes generous
T26222 2808-2809 DT denotes a
T26221 2803-2807 VB denotes were
T26220 2791-2802 NN denotes fibroblasts
T26219 2786-2790 NN denotes Lung
T26218 2780-2784 NN denotes ATCC
T26217 2775-2779 IN denotes from
T26216 2766-2774 VB denotes obtained
T26215 2761-2765 VB denotes were
T26214 2755-2760 NN denotes cells
T26213 2748-2754 NN denotes Jurkat
T26212 2733-2747 JJ denotes FADD-deficient
T26211 2729-2732 CC denotes and
T26210 2724-2728 NN denotes L929
T26209 2718-2723 NN denotes Lines
T26208 2713-2717 NN denotes Cell
T25245 1096-1101 NNP denotes Sigma
T25244 1091-1095 IN denotes from
T25243 1086-1090 VB denotes were
T25242 1077-1085 NN denotes reagents
T25241 1071-1076 JJ denotes other
T25240 1067-1070 DT denotes All
T25239 1056-1066 NNP denotes Peprotech.
T25238 1053-1055 CC denotes or
T25237 1044-1052 NNP denotes Sciences
T25236 1039-1043 NNP denotes Cell
T25235 1034-1038 IN denotes from
T25234 1029-1033 VB denotes were
T25233 1027-1028 -RRB- denotes )
T25232 1022-1027 NN denotes ng/ml
T25231 1019-1021 CD denotes 50
T25230 1018-1019 -LRB- denotes (
T25229 1012-1017 NN denotes IGF-1
T25228 1008-1011 CC denotes and
T25227 1006-1007 -COMMA- denotes ,
T25226 1005-1006 -RRB- denotes )
T25225 1000-1005 NN denotes ng/ml
T25224 997-999 CD denotes 20
T25223 996-997 -LRB- denotes (
T25222 988-995 NN denotes PDGF-BB
T25221 986-987 -COMMA- denotes ,
T25220 985-986 -RRB- denotes )
T25219 980-985 NN denotes ng/ml
T25218 977-979 CD denotes 50
T25217 976-977 -LRB- denotes (
T25216 972-975 NN denotes EGF
T25215 970-971 -COMMA- denotes ,
T25214 969-970 -RRB- denotes )
T25213 964-969 NN denotes ng/ml
T25212 961-963 CD denotes 25
T25211 960-961 -LRB- denotes (
T25210 955-959 NN denotes bFGF
T25209 949-954 JJ denotes human
T25208 947-948 -COMMA- denotes ,
T25207 946-947 -RRB- denotes )
T25206 941-946 NN denotes ng/ml
T25205 938-940 CD denotes 10
T25204 937-938 -LRB- denotes (
T25203 932-936 NN denotes TNFα
T25202 926-931 NN denotes mouse
T25201 922-925 CC denotes and
T25200 916-921 NNP denotes Human
T25199 908-915 NNP denotes Bachem.
T25198 903-907 IN denotes from
T25197 893-902 VB denotes purchased
T25196 889-892 VB denotes was
T25195 887-888 -RRB- denotes )
T25194 885-887 NN denotes µM
T25193 879-884 CD denotes 20–30
T25192 878-879 -LRB- denotes (
T25191 869-877 NN denotes zVAD.fmk
T25190 859-868 NN denotes inhibitor
T25189 847-858 NN denotes Pan-caspase
T25188 244-245 -COLON- denotes :
T25187 233-244 NN denotes experiments
T25186 229-232 DT denotes the
T25185 226-228 IN denotes in
T25184 221-225 VB denotes used
T25183 216-220 VB denotes were
T25182 214-215 -RRB- denotes )
T25181 202-214 NN denotes text/figures
T25180 198-201 DT denotes the
T25179 195-197 IN denotes in
T25178 185-194 VB denotes specified
T25177 175-184 RB denotes otherwise
T25176 168-174 IN denotes unless
T25175 167-168 -LRB- denotes (
T25174 152-166 NN denotes concentrations
T25173 146-151 JJ denotes final
T25172 142-145 CC denotes and
T25171 133-141 NN denotes reagents
T25170 123-132 VB denotes following
T25169 119-122 DT denotes The
T25168 116-117 -RRB- denotes ]
T25167 114-116 CD denotes 24
T25166 113-114 -LRB- denotes [
T25165 111-112 -COMMA- denotes ,
T25164 110-111 -RRB- denotes ]
T25163 108-110 CD denotes 23
T25162 107-108 -LRB- denotes [
T25161 97-106 VB denotes described
T25160 86-96 RB denotes previously
T25159 83-85 IN denotes as
T25158 71-82 VB denotes synthesized
T25157 66-70 VB denotes were
T25156 58-65 NN denotes analogs
T25155 46-57 NN denotes Necrostatin
T25154 36-45 NN denotes Chemicals
T25153 32-35 CC denotes and
T25152 23-31 NN denotes Reagents
T25647 1370-1378 NN denotes strategy
T25646 1365-1369 JJ denotes same
T25645 1361-1364 DT denotes the
T25644 1355-1360 VB denotes using
T25643 1345-1354 VB denotes generated
T25642 1340-1344 VB denotes were
T25641 1331-1339 NN denotes Myr-Akt1
T25640 1328-1330 IN denotes of
T25639 1319-1327 NN denotes versions
T25638 1312-1318 JJ denotes Mutant
T25637 1309-1310 -RRB- denotes )
T25636 1299-1309 NN denotes Invitrogen
T25635 1298-1299 -LRB- denotes (
T25634 1291-1297 NN denotes vector
T25633 1280-1290 JJ denotes retroviral
T25632 1269-1279 NN denotes pMSCV-puro
T25631 1266-1268 IN denotes of
T25630 1260-1265 NN denotes sites
T25629 1254-1259 NN denotes EcoRI
T25628 1250-1253 CC denotes and
T25627 1244-1249 NN denotes BglII
T25626 1240-1243 DT denotes the
T25625 1235-1239 IN denotes into
T25624 1225-1234 VB denotes subcloned
T25623 1221-1224 CC denotes and
T25622 1217-1220 NN denotes PCR
T25621 1214-1216 IN denotes by
T25620 1204-1213 VB denotes amplified
T25619 1200-1203 VB denotes was
T25618 1186-1199 NN denotes Myr-Akt1-FLAG
T25617 1183-1184 -RRB- denotes ]
T25616 1181-1183 CD denotes 52
T25615 1180-1181 -LRB- denotes [
T25614 1170-1179 VB denotes described
T25613 1165-1169 VB denotes been
T25612 1161-1164 VB denotes has
T25611 1159-1160 -COMMA- denotes ,
T25610 1156-1159 NN denotes tag
T25609 1151-1155 NN denotes FLAG
T25608 1140-1150 JJ denotes c-terminal
T25607 1129-1139 VB denotes containing
T25606 1127-1128 -COMMA- denotes ,
T25605 1119-1127 NN denotes Myr-Akt1
T25604 1116-1118 IN denotes of
T25603 1108-1115 NN denotes Cloning
T25602 1104-1107 NN denotes DNA
T27975 6642-6650 NN denotes ELISAOne
R17099 T25152 T25153 arg1Of Reagents,and
R17100 T25154 T25153 arg2Of Chemicals,and
R17101 T25156 T25155 arg1Of analogs,Necrostatin
R17102 T25156 T25157 arg1Of analogs,were
R17103 T25156 T25158 arg2Of analogs,synthesized
R17104 T25158 T25157 arg2Of synthesized,were
R17105 T25158 T25159 arg1Of synthesized,as
R17106 T25158 T25165 arg1Of synthesized,","
R17107 T25158 T25166 arg1Of synthesized,[
R17108 T25161 T25160 arg1Of described,previously
R17109 T25163 T25159 arg2Of 23,as
R17110 T25163 T25161 arg1Of 23,described
R17111 T25163 T25162 arg2Of 23,[
R17112 T25164 T25162 arg3Of ],[
R17113 T25167 T25166 arg2Of 24,[
R17114 T25168 T25166 arg3Of ],[
R17115 T25171 T25170 arg1Of reagents,following
R17116 T25171 T25172 arg1Of reagents,and
R17117 T25172 T25169 arg1Of and,The
R17118 T25172 T25175 arg1Of and,(
R17119 T25174 T25172 arg2Of concentrations,and
R17120 T25174 T25173 arg1Of concentrations,final
R17121 T25178 T25176 arg2Of specified,unless
R17122 T25178 T25177 arg1Of specified,otherwise
R17123 T25178 T25179 arg1Of specified,in
R17124 T25181 T25180 arg1Of text/figures,the
R17125 T25181 T25182 arg1Of text/figures,)
R17126 T25181 T25183 arg1Of text/figures,were
R17127 T25181 T25184 arg2Of text/figures,used
R17128 T25184 T25175 arg2Of used,(
R17129 T25184 T25176 arg1Of used,unless
R17130 T25184 T25183 arg2Of used,were
R17131 T25184 T25185 arg1Of used,in
R17132 T25187 T25185 arg2Of experiments,in
R17133 T25187 T25186 arg1Of experiments,the
R17134 T25188 T25175 arg3Of :,(
R17135 T25191 T25189 arg1Of zVAD.fmk,Pan-caspase
R17136 T25191 T25190 arg1Of zVAD.fmk,inhibitor
R17137 T25191 T25192 arg1Of zVAD.fmk,(
R17138 T25191 T25196 arg1Of zVAD.fmk,was
R17139 T25191 T25197 arg2Of zVAD.fmk,purchased
R17140 T25194 T25192 arg2Of µM,(
R17141 T25194 T25193 arg1Of µM,20–30
R17142 T25195 T25192 arg3Of ),(
R17143 T25197 T25196 arg2Of purchased,was
R17144 T25197 T25198 arg1Of purchased,from
R17145 T25200 T25198 arg2Of Human,from
R17146 T25200 T25199 arg1Of Human,Bachem.
R17147 T25203 T25201 arg1Of TNFα,and
R17148 T25203 T25202 arg1Of TNFα,mouse
R17149 T25203 T25204 arg1Of TNFα,(
R17150 T25203 T25208 arg1Of TNFα,","
R17151 T25203 T25238 arg1Of TNFα,or
R17152 T25206 T25204 arg2Of ng/ml,(
R17153 T25206 T25205 arg1Of ng/ml,10
R17154 T25207 T25204 arg3Of ),(
R17155 T25210 T25209 arg1Of bFGF,human
R17156 T25210 T25211 arg1Of bFGF,(
R17157 T25210 T25215 arg1Of bFGF,","
R17158 T25213 T25211 arg2Of ng/ml,(
R17159 T25213 T25212 arg1Of ng/ml,25
R17160 T25214 T25211 arg3Of ),(
R17161 T25215 T25221 arg1Of ",",","
R17162 T25216 T25215 arg2Of EGF,","
R17163 T25216 T25217 arg1Of EGF,(
R17164 T25219 T25217 arg2Of ng/ml,(
R17165 T25219 T25218 arg1Of ng/ml,50
R17166 T25220 T25217 arg3Of ),(
R17167 T25221 T25228 arg1Of ",",and
R17168 T25222 T25221 arg2Of PDGF-BB,","
R17169 T25222 T25223 arg1Of PDGF-BB,(
R17170 T25225 T25223 arg2Of ng/ml,(
R17171 T25225 T25224 arg1Of ng/ml,20
R17172 T25226 T25223 arg3Of ),(
R17173 T25228 T25227 arg1Of and,","
R17174 T25228 T25234 arg1Of and,were
R17175 T25228 T25235 arg1Of and,from
R17176 T25229 T25228 arg2Of IGF-1,and
R17177 T25229 T25230 arg1Of IGF-1,(
R17178 T25232 T25230 arg2Of ng/ml,(
R17179 T25232 T25231 arg1Of ng/ml,50
R17180 T25233 T25230 arg3Of ),(
R17181 T25234 T25208 arg2Of were,","
R17182 T25235 T25234 arg2Of from,were
R17183 T25237 T25235 arg2Of Sciences,from
R17184 T25237 T25236 arg1Of Sciences,Cell
R17185 T25238 T25243 arg1Of or,were
R17186 T25238 T25244 arg1Of or,from
R17187 T25242 T25238 arg2Of reagents,or
R17188 T25242 T25239 arg1Of reagents,Peprotech.
R17189 T25242 T25240 arg1Of reagents,All
R17190 T25242 T25241 arg1Of reagents,other
R17191 T25243 T25196 modOf were,was
R17192 T25244 T25243 arg2Of from,were
R17193 T25245 T25244 arg2Of Sigma,from
R17465 T25603 T25604 arg1Of Cloning,of
R17466 T25603 T25606 arg1Of Cloning,","
R17467 T25603 T25607 arg1Of Cloning,containing
R17468 T25603 T25612 arg1Of Cloning,has
R17469 T25603 T25613 arg1Of Cloning,been
R17470 T25603 T25614 arg2Of Cloning,described
R17471 T25605 T25604 arg2Of Myr-Akt1,of
R17472 T25610 T25607 arg2Of tag,containing
R17473 T25610 T25608 arg1Of tag,c-terminal
R17474 T25610 T25609 arg1Of tag,FLAG
R17475 T25614 T25611 arg1Of described,","
R17476 T25614 T25612 arg2Of described,has
R17477 T25614 T25613 arg2Of described,been
R17478 T25614 T25615 arg1Of described,[
R17479 T25616 T25615 arg2Of 52,[
R17480 T25617 T25615 arg3Of ],[
R17481 T25618 T25619 arg1Of Myr-Akt1-FLAG,was
R17482 T25618 T25620 arg2Of Myr-Akt1-FLAG,amplified
R17483 T25618 T25624 arg2Of Myr-Akt1-FLAG,subcloned
R17484 T25620 T25623 arg1Of amplified,and
R17485 T25622 T25620 arg1Of PCR,amplified
R17486 T25622 T25621 arg2Of PCR,by
R17487 T25623 T25619 arg2Of and,was
R17488 T25624 T25623 arg2Of subcloned,and
R17489 T25624 T25625 arg1Of subcloned,into
R17490 T25627 T25628 arg1Of BglII,and
R17491 T25629 T25628 arg2Of EcoRI,and
R17492 T25630 T25625 arg2Of sites,into
R17493 T25630 T25626 arg1Of sites,the
R17494 T25630 T25627 arg1Of sites,BglII
R17495 T25630 T25629 arg1Of sites,EcoRI
R17496 T25630 T25631 arg1Of sites,of
R17497 T25634 T25631 arg2Of vector,of
R17498 T25634 T25632 arg1Of vector,pMSCV-puro
R17499 T25634 T25633 arg1Of vector,retroviral
R17500 T25634 T25635 arg1Of vector,(
R17501 T25636 T25635 arg2Of Invitrogen,(
R17502 T25637 T25635 arg3Of ),(
R17503 T25639 T25638 arg1Of versions,Mutant
R17504 T25639 T25640 arg1Of versions,of
R17505 T25639 T25642 arg1Of versions,were
R17506 T25639 T25643 arg2Of versions,generated
R17507 T25641 T25640 arg2Of Myr-Akt1,of
R17508 T25643 T25642 arg2Of generated,were
R17509 T25644 T25643 arg3Of using,generated
R17510 T25647 T25644 arg2Of strategy,using
R17511 T25647 T25645 arg1Of strategy,the
R17512 T25647 T25646 arg1Of strategy,same
R17780 T26016 T26015 arg1Of Primers,QPCR
R17781 T26018 T26017 arg1Of TNFα,Mouse
R17782 T26018 T26019 arg1Of TNFα,:
R17783 T26021 T26020 arg1Of 5′-CCCTCACACTCAGATCATCTTCT-3′,forward
R17784 T26021 T26022 arg1Of 5′-CCCTCACACTCAGATCATCTTCT-3′,","
R17785 T26023 T26022 arg2Of reverse,","
R17786 T26024 T26021 arg1Of 5′-GCTACGACGTGGGCTACAG-3′,5′-CCCTCACACTCAGATCATCTTCT-3′
R17787 T26024 T26023 arg1Of 5′-GCTACGACGTGGGCTACAG-3′,reverse
R17788 T26024 T26025 arg1Of 5′-GCTACGACGTGGGCTACAG-3′,;
R17789 T26025 T26019 arg2Of ;,:
R17790 T26025 T26028 arg1Of ;,:
R17791 T26027 T26025 arg2Of 18S,;
R17792 T26027 T26026 arg1Of 18S,mouse
R17793 T26030 T26029 arg1Of 5-′,forward
R17794 T26031 T26028 arg2Of ATAACAGGTCTGTGATGCCCTTAG-3,:
R17795 T26031 T26030 arg1Of ATAACAGGTCTGTGATGCCCTTAG-3,5-′
R17796 T26031 T26032 arg1Of ATAACAGGTCTGTGATGCCCTTAG-3,","
R17797 T26031 T26035 arg1Of ATAACAGGTCTGTGATGCCCTTAG-3,;
R17798 T26034 T26032 arg2Of 5′-CTAAACCATCCAATCGGTAGTAGC-3′,","
R17799 T26034 T26033 arg1Of 5′-CTAAACCATCCAATCGGTAGTAGC-3′,reverse
R17800 T26037 T26035 arg2Of TNFα,;
R17801 T26037 T26036 arg1Of TNFα,human
R17802 T26037 T26038 arg1Of TNFα,:
R17803 T26040 T26039 arg1Of 5′-,forward
R17804 T26041 T26038 arg2Of ATGAGCACTGAAAGCATGATCC-3′,:
R17805 T26041 T26040 arg1Of ATGAGCACTGAAAGCATGATCC-3′,5′-
R17806 T26041 T26042 arg1Of ATGAGCACTGAAAGCATGATCC-3′,","
R17807 T26041 T26045 arg1Of ATGAGCACTGAAAGCATGATCC-3′,:
R17808 T26044 T26042 arg2Of TNFα,","
R17809 T26044 T26043 arg1Of TNFα,human
R17810 T26047 T26045 arg2Of 5′-GAGGGCTGATTAGAGAGAGGTC-3′,:
R17811 T26047 T26046 arg1Of 5′-GAGGGCTGATTAGAGAGAGGTC-3′,reverse
R17812 T26047 T26048 arg1Of 5′-GAGGGCTGATTAGAGAGAGGTC-3′,;
R17813 T26050 T26048 arg2Of 18S,;
R17814 T26050 T26049 arg1Of 18S,human
R17815 T26050 T26051 arg1Of 18S,:
R17816 T26053 T26052 arg1Of 5′-,forward
R17817 T26054 T26053 arg1Of CAGCCACCCGAGATTGAGCA,5′-
R17818 T26054 T26055 arg1Of CAGCCACCCGAGATTGAGCA,-3
R17819 T26054 T26056 arg1Of CAGCCACCCGAGATTGAGCA,","
R17820 T26056 T26051 arg2Of ",",:
R17821 T26056 T26059 arg1Of ",",:
R17822 T26058 T26056 arg2Of 18S,","
R17823 T26058 T26057 arg1Of 18S,human
R17824 T26061 T26059 arg2Of 5′-TAGTAGCGACGGGCGGTGTG-3′,:
R17825 T26061 T26060 arg1Of 5′-TAGTAGCGACGGGCGGTGTG-3′,reverse
R17916 T26221 T26233 arg1Of were,[
R17926 T26231 T26230 arg1Of University,Tufts
R17927 T26232 T26229 arg3Of ),(
R17928 T26234 T26233 arg2Of 53,[
R17929 T26235 T26233 arg3Of ],[
R17930 T26236 T26237 arg1Of J774A.1,(
R17931 T26238 T26237 arg2Of ATCC,(
R17932 T26239 T26237 arg3Of ),(
R17933 T26240 T26236 arg1Of cells,J774A.1
R17934 T26240 T26241 arg1Of cells,and
R17935 T26241 T26247 arg1Of and,were
R17936 T26244 T26243 arg2Of ATCC,(
R17937 T26245 T26243 arg3Of ),(
R17938 T26246 T26241 arg2Of cells,and
R17939 T26246 T26242 arg1Of cells,RAW264.7
R17940 T26246 T26243 arg1Of cells,(
R17941 T26249 T26247 arg2Of gifts,were
R17942 T26249 T26248 arg1Of gifts,generous
R17943 T26249 T26250 arg1Of gifts,of
R17944 T26252 T26251 arg1Of Yuan,Junying
R17945 T26252 T26253 arg1Of Yuan,(
R17946 T26252 T26257 arg1Of Yuan,and
R17947 T26255 T26253 arg2Of University,(
R17948 T26255 T26254 arg1Of University,Harvard
R17949 T26256 T26253 arg3Of ),(
R17950 T26257 T26250 arg2Of and,of
R17951 T26259 T26257 arg2Of Poltorak,and
R17952 T26259 T26258 arg1Of Poltorak,Alexander
R17953 T26259 T26260 arg1Of Poltorak,(
R17954 T26259 T26264 arg1Of Poltorak,","
R17955 T26259 T26265 arg1Of Poltorak,respectively
R17956 T26262 T26260 arg2Of University,(
R17957 T26262 T26261 arg1Of University,Tufts
R17958 T26263 T26260 arg3Of ),(
R17959 T26266 T26267 arg1Of Cells,were
R17960 T26266 T26268 arg2Of Cells,maintained
R17961 T26268 T26267 arg2Of maintained,were
R17962 T26268 T26269 arg1Of maintained,in
R17963 T26270 T26269 arg2Of DMEM,in
R17964 T26270 T26271 arg2Of DMEM,supplemented
R17965 T26271 T26272 arg1Of supplemented,with
R17966 T26273 T26274 arg1Of 10,%
R17967 T26277 T26273 arg1Of serum,10
R17968 T26277 T26275 arg1Of serum,fetal
R17969 T26277 T26276 arg1Of serum,bovine
R17970 T26277 T26278 arg1Of serum,(
R17971 T26277 T26281 arg1Of serum,and
R17972 T26279 T26278 arg2Of FBS,(
R17973 T26280 T26278 arg3Of ),(
R17974 T26281 T26272 arg2Of and,with
R17975 T26282 T26283 arg1Of 1,%
R17976 T26285 T26281 arg2Of mixture,and
R17977 T26285 T26282 arg1Of mixture,1
R17978 T26285 T26284 arg1Of mixture,antibiotic-antimycotic
R17979 T26285 T26286 arg1Of mixture,(
R17980 T26287 T26286 arg2Of Invitrogen,(
R17981 T26288 T26286 arg3Of ),(
R17982 T26293 T26289 arg1Of media,The
R17983 T26293 T26290 arg1Of media,mouse
R17984 T26293 T26291 arg1Of media,lung
R17985 T26293 T26292 arg1Of media,fibroblast
R17986 T26293 T26294 arg1Of media,was
R17987 T26293 T26296 arg2Of media,supplemented
R17988 T26296 T26294 arg2Of supplemented,was
R17989 T26296 T26295 arg1Of supplemented,additionally
R17990 T26296 T26297 arg1Of supplemented,with
R17991 T26298 T26299 arg1Of L-glutamine,","
R17992 T26299 T26304 arg1Of ",",and
R17993 T26302 T26299 arg2Of acids,","
R17994 T26302 T26300 arg1Of acids,non-essential
R17995 T26302 T26301 arg1Of acids,amino
R17996 T26304 T26297 arg2Of and,with
R17997 T26304 T26303 arg1Of and,","
R17998 T26306 T26304 arg2Of pyruvate,and
R17999 T26306 T26305 arg1Of pyruvate,sodium
R18000 T26308 T26307 arg1Of cells,Jurkat
R18001 T26308 T26309 arg1Of cells,were
R18002 T26308 T26310 arg2Of cells,maintained
R18003 T26310 T26309 arg2Of maintained,were
R18004 T26310 T26311 arg1Of maintained,in
R18005 T26312 T26313 arg1Of RPMI1640,","
R18006 T26312 T26314 arg2Of RPMI1640,supplemented
R18007 T26312 T26322 arg1Of RPMI1640,and
R18008 T26314 T26315 arg1Of supplemented,with
R18009 T26314 T26319 arg1Of supplemented,(
R18010 T26316 T26317 arg1Of 10,%
R18011 T26318 T26315 arg2Of FetalPlex,with
R18012 T26318 T26316 arg1Of FetalPlex,10
R18013 T26320 T26319 arg2Of Gemini,(
R18014 T26321 T26319 arg3Of ),(
R18015 T26322 T26311 arg2Of and,in
R18016 T26322 T26325 arg1Of and,antibiotic-antimycotic
R18017 T26324 T26322 arg2Of %,and
R18018 T26324 T26323 arg1Of %,1
R18149 T26491 T26489 arg1Of Experiments,Cell
R18150 T26491 T26490 arg1Of Experiments,Viability
R18151 T26492 T26493 arg1Of Cells,were
R18152 T26492 T26494 arg2Of Cells,seeded
R18153 T26492 T26509 arg2Of Cells,treated
R18154 T26494 T26495 arg1Of seeded,into
R18155 T26494 T26502 arg1Of seeded,at
R18156 T26494 T26508 arg1Of seeded,and
R18157 T26498 T26495 arg2Of bottom,into
R18158 T26498 T26496 arg1Of bottom,white
R18159 T26498 T26497 arg1Of bottom,clear
R18160 T26498 T26501 arg2Of bottom,plates
R18161 T26499 T26501 arg1Of 96,plates
R18162 T26501 T26500 arg1Of plates,well
R18163 T26504 T26502 arg2Of density,at
R18164 T26504 T26503 arg1Of density,the
R18165 T26504 T26505 arg1Of density,of
R18166 T26507 T26505 arg2Of cells/well,of
R18167 T26507 T26506 arg1Of cells/well,1×104
R18168 T26508 T26493 arg2Of and,were
R18169 T26509 T26508 arg2Of treated,and
R18170 T26509 T26510 arg1Of treated,as
R18171 T26511 T26510 arg2Of described,as
R18172 T26511 T26512 arg1Of described,for
R18173 T26515 T26512 arg2Of experiments,for
R18174 T26515 T26513 arg1Of experiments,western
R18175 T26515 T26514 arg1Of experiments,blot
R18176 T26517 T26516 arg1Of viability,Cell
R18177 T26517 T26518 arg1Of viability,was
R18178 T26517 T26519 arg2Of viability,determined
R18179 T26519 T26518 arg2Of determined,was
R18180 T26520 T26519 arg3Of using,determined
R18181 T26524 T26520 arg2Of Assay,using
R18182 T26524 T26521 arg1Of Assay,CellTiter-Glo
R18183 T26524 T26522 arg1Of Assay,Cell
R18184 T26524 T26523 arg1Of Assay,Viability
R18185 T26524 T26525 arg1Of Assay,(
R18186 T26526 T26525 arg2Of Promega,(
R18187 T26527 T26525 arg3Of ),(
R18188 T26528 T26529 arg1Of Experiments,were
R18189 T26528 T26530 arg2Of Experiments,performed
R18190 T26528 T26534 arg1Of Experiments,triplicate
R18191 T26530 T26529 arg2Of performed,were
R18192 T26530 T26531 arg1Of performed,in
R18193 T26530 T26533 arg1Of performed,or
R18194 T26532 T26531 arg2Of duplicate,in
R18195 T26534 T26533 arg2Of triplicate,or
R18196 T26535 T26536 arg1Of Viability,of
R18197 T26535 T26541 arg1Of Viability,was
R18198 T26535 T26542 arg2Of Viability,set
R18199 T26540 T26536 arg2Of cells,of
R18200 T26540 T26537 arg1Of cells,the
R18201 T26540 T26538 arg1Of cells,control
R18202 T26540 T26539 arg1Of cells,untreated
R18203 T26542 T26541 arg2Of set,was
R18204 T26542 T26543 arg1Of set,as
R18205 T26545 T26543 arg2Of %,as
R18206 T26545 T26544 arg1Of %,100
R18207 T26547 T26546 arg1Of viability,Relative
R18208 T26547 T26548 arg1Of viability,of
R18209 T26547 T26551 arg1Of viability,induced
R18210 T26547 T26553 arg1Of viability,undergo
R18211 T26547 T26556 arg2Of viability,treated
R18212 T26547 T26567 arg1Of viability,was
R18213 T26547 T26568 arg2Of viability,determined
R18214 T26547 T26570 arg2Of viability,plotted
R18215 T26547 T26572 arg1Of viability,exclude
R18216 T26549 T26548 arg2Of cells,of
R18217 T26551 T26552 modOf induced,to
R18218 T26551 T26566 arg1Of induced,","
R18219 T26553 T26555 arg1Of undergo,and
R18220 T26554 T26553 arg2Of necroptosis,undergo
R18221 T26555 T26552 arg1Of and,to
R18222 T26556 T26555 arg2Of treated,and
R18223 T26556 T26557 arg1Of treated,with
R18224 T26559 T26557 arg2Of compound,with
R18225 T26559 T26558 arg1Of compound,the
R18226 T26559 T26560 arg1Of compound,relative
R18227 T26560 T26561 arg1Of relative,to
R18228 T26565 T26561 arg2Of cells,to
R18229 T26565 T26562 arg1Of cells,the
R18230 T26565 T26563 arg1Of cells,control
R18231 T26565 T26564 arg1Of cells,compound-treated
R18232 T26566 T26550 arg1Of ",",","
R18233 T26568 T26569 arg1Of determined,and
R18234 T26569 T26566 arg2Of and,","
R18235 T26569 T26567 arg2Of and,was
R18236 T26570 T26569 arg2Of plotted,and
R18237 T26572 T26570 arg3Of exclude,plotted
R18238 T26572 T26571 arg1Of exclude,to
R18239 T26575 T26572 arg2Of effects,exclude
R18240 T26575 T26573 arg1Of effects,the
R18241 T26575 T26574 arg1Of effects,possible
R18242 T26575 T26576 arg1Of effects,of
R18243 T26578 T26576 arg2Of toxicity,of
R18244 T26578 T26577 arg1Of toxicity,non-specific
R18245 T26578 T26579 arg1Of toxicity,of
R18246 T26582 T26579 arg2Of molecules,of
R18247 T26582 T26580 arg1Of molecules,the
R18248 T26582 T26581 arg1Of molecules,small
R18350 T26749 T26748 arg1Of Knockdown,siRNA
R18351 T26750 T26751 arg1Of siRNAs,were
R18352 T26750 T26752 arg2Of siRNAs,purchased
R18353 T26752 T26751 arg2Of purchased,were
R18354 T26752 T26753 arg1Of purchased,from
R18355 T26755 T26754 arg1Of Mouse,Dharmacon.
R18356 T26758 T26755 arg1Of protein,Mouse
R18357 T26758 T26756 arg1Of protein,ribosomal
R18358 T26758 T26757 arg1Of protein,S6
R18359 T26758 T26759 arg1Of protein,(
R18360 T26758 T26764 arg1Of protein,","
R18361 T26760 T26761 arg1Of L-040893-00,and
R18362 T26761 T26759 arg2Of and,(
R18363 T26762 T26761 arg2Of L-045791-00,and
R18364 T26763 T26759 arg3Of ),(
R18365 T26764 T26770 arg1Of ",",","
R18366 T26766 T26764 arg2Of Akt1,","
R18367 T26766 T26765 arg1Of Akt1,mouse
R18368 T26766 T26767 arg1Of Akt1,(
R18369 T26768 T26767 arg2Of L-040709-00,(
R18370 T26769 T26767 arg3Of ),(
R18371 T26770 T26788 arg1Of ",",","
R18372 T26772 T26771 arg1Of Akt2,mouse
R18373 T26772 T26773 arg1Of Akt2,(
R18374 T26772 T26776 arg1Of Akt2,","
R18375 T26774 T26773 arg2Of L-040782-00,(
R18376 T26775 T26773 arg3Of ),(
R18377 T26776 T26782 arg1Of ",",","
R18378 T26778 T26776 arg2Of Akt3,","
R18379 T26778 T26777 arg1Of Akt3,mouse
R18380 T26778 T26779 arg1Of Akt3,(
R18381 T26780 T26779 arg2Of L-040891-00,(
R18382 T26781 T26779 arg3Of ),(
R18383 T26782 T26770 arg2Of ",",","
R18384 T26784 T26782 arg2Of mTOR,","
R18385 T26784 T26783 arg1Of mTOR,mouse
R18386 T26784 T26785 arg1Of mTOR,(
R18387 T26786 T26785 arg2Of L-065427-00,(
R18388 T26787 T26785 arg3Of ),(
R18389 T26788 T26753 arg2Of ",",from
R18390 T26788 T26794 arg1Of ",",","
R18391 T26790 T26788 arg2Of PDK1,","
R18392 T26790 T26789 arg1Of PDK1,mouse
R18393 T26790 T26791 arg1Of PDK1,(
R18394 T26792 T26791 arg2Of L-040658-00,(
R18395 T26793 T26791 arg3Of ),(
R18396 T26796 T26795 arg1Of control,non-coding
R18397 T26796 T26797 arg1Of control,(
R18398 T26796 T26800 arg1Of control,","
R18399 T26798 T26797 arg2Of D-001810-10-05,(
R18400 T26799 T26797 arg3Of ),(
R18401 T26800 T26806 arg1Of ",",","
R18402 T26802 T26800 arg2Of Mapk8,","
R18403 T26802 T26801 arg1Of Mapk8,mouse
R18404 T26802 T26803 arg1Of Mapk8,(
R18405 T26804 T26803 arg2Of J-040128-05,(
R18406 T26805 T26803 arg3Of ),(
R18407 T26806 T26794 arg2Of ",",","
R18408 T26806 T26819 modOf ",",NA w
R18409 T26808 T26806 arg2Of Mapk9,","
R18410 T26808 T26807 arg1Of Mapk9,mouse
R18411 T26808 T26809 arg1Of Mapk9,(
R18412 T26810 T26809 arg2Of J-040134-05,(
R18413 T26811 T26809 arg3Of ),(
R18414 T26816 T26815 arg2Of L-043776-00,(
R18415 T26817 T26815 arg3Of ),(
R18416 T26818 T26813 arg1Of . siR,mouse
R18417 T26818 T26814 arg1Of . siR,Jun
R18418 T26818 T26815 arg1Of . siR,(
R18419 T26818 T26819 arg1Of . siR,NA w
R18420 T26818 T26820 arg2Of . siR,ere transfe
R18421 T26820 T26812 arg1Of ere transfe,","
R18422 T26820 T26819 arg2Of ere transfe,NA w
R18423 T26820 T26821 modOf ere transfe,cted
R18424 T26820 T26827 arg1Of ere transfe,i
R18425 T26820 T26828 arg1Of ere transfe,"trogen), "
R18426 T26823 T26821 arg2Of NAiMAX ,cted
R18427 T26823 T26822 arg1Of NAiMAX ,using R
R18428 T26823 T26824 arg1Of NAiMAX ,r
R18429 T26825 T26824 arg2Of eagent (In,r
R18430 T26826 T26824 arg3Of v,r
R18431 T26829 T26828 arg2Of ac,"trogen), "
R18432 T26831 T26829 arg2Of ufacturer’s rec,ac
R18433 T26831 T26830 arg1Of ufacturer’s rec,cording to man
R18434 T26834 T26832 arg2Of hr,After
R18435 T26834 T26833 arg1Of hr,72
R18436 T26836 T26837 arg1Of cells,were
R18437 T26836 T26838 arg2Of cells,treated
R18438 T26838 T26832 arg1Of treated,After
R18439 T26838 T26835 arg1Of treated,","
R18440 T26838 T26837 arg2Of treated,were
R18441 T26838 T26839 arg1Of treated,with
R18442 T26838 T26843 arg1Of treated,for
R18443 T26840 T26841 arg1Of zVAD.fmk,or
R18444 T26841 T26839 arg2Of or,with
R18445 T26842 T26841 arg2Of TNFα,or
R18446 T26845 T26844 arg1Of hr,9
R18447 T26845 T26846 arg1Of hr,(
R18448 T26845 T26852 arg1Of hr,or
R18449 T26847 T26848 arg1Of RNA,or
R18450 T26848 T26846 arg2Of or,(
R18451 T26850 T26848 arg2Of blot,or
R18452 T26850 T26849 arg1Of blot,Western
R18453 T26851 T26846 arg3Of ),(
R18454 T26852 T26843 arg2Of or,for
R18455 T26854 T26852 arg2Of hr,or
R18456 T26854 T26853 arg1Of hr,24
R18457 T26854 T26855 arg1Of hr,(
R18458 T26857 T26855 arg2Of viability,(
R18459 T26857 T26856 arg1Of viability,cell
R18460 T26858 T26855 arg3Of ),(
R18601 T27102 T27101 arg1Of Blot,Western
R18602 T27105 T27103 arg2Of blot,For
R18603 T27105 T27104 arg1Of blot,Western
R18604 T27109 T27107 arg1Of cells,4×105
R18605 T27109 T27108 arg1Of cells,adherent
R18606 T27109 T27110 arg1Of cells,(
R18607 T27109 T27115 arg1Of cells,were
R18608 T27109 T27116 arg2Of cells,seeded
R18609 T27113 T27110 arg2Of cells,(
R18610 T27113 T27111 arg1Of cells,1×106
R18611 T27113 T27112 arg1Of cells,Jurkat
R18612 T27114 T27110 arg3Of ),(
R18613 T27116 T27103 arg1Of seeded,For
R18614 T27116 T27106 arg1Of seeded,","
R18615 T27116 T27115 arg2Of seeded,were
R18616 T27116 T27117 arg1Of seeded,into
R18617 T27120 T27117 arg2Of dishes,into
R18618 T27120 T27118 arg1Of dishes,35
R18619 T27120 T27119 arg1Of dishes,mm2
R18620 T27123 T27121 arg2Of hr,After
R18621 T27123 T27122 arg1Of hr,24–48
R18622 T27125 T27126 arg1Of cells,were
R18623 T27125 T27127 arg2Of cells,stimulated
R18624 T27127 T27121 arg1Of stimulated,After
R18625 T27127 T27124 arg1Of stimulated,","
R18626 T27127 T27126 arg2Of stimulated,were
R18627 T27127 T27128 arg1Of stimulated,with
R18628 T27129 T27130 arg1Of 30,µM
R18629 T27131 T27129 arg1Of zVAD.fmk,30
R18630 T27131 T27132 arg1Of zVAD.fmk,or
R18631 T27132 T27128 arg2Of or,with
R18632 T27136 T27132 arg2Of TNFα,or
R18633 T27136 T27133 arg1Of TNFα,10
R18634 T27136 T27134 arg1Of TNFα,ng/ml
R18635 T27136 T27135 arg1Of TNFα,mouse
R18636 T27138 T27137 arg2Of treatments,For
R18637 T27138 T27139 arg1Of treatments,under
R18638 T27142 T27139 arg2Of conditions,under
R18639 T27142 T27140 arg1Of conditions,serum
R18640 T27142 T27141 arg1Of conditions,free
R18641 T27144 T27145 arg1Of cells,were
R18642 T27145 T27137 arg1Of were,For
R18643 T27145 T27143 arg1Of were,","
R18644 T27146 T27145 arg2Of serum,were
R18645 T27146 T27147 arg2Of serum,starved
R18646 T27147 T27148 arg1Of starved,for
R18647 T27150 T27148 arg2Of hr,for
R18648 T27150 T27149 arg1Of hr,24
R18649 T27150 T27151 arg1Of hr,prior
R18650 T27151 T27152 arg1Of prior,to
R18651 T27154 T27152 arg2Of addition,to
R18652 T27154 T27153 arg1Of addition,the
R18653 T27154 T27155 arg1Of addition,of
R18654 T27154 T27158 arg1Of addition,","
R18655 T27157 T27155 arg2Of factors,of
R18656 T27157 T27156 arg1Of factors,growth
R18657 T27159 T27160 arg1Of 20,µM
R18658 T27161 T27159 arg1Of zVAD.fmk,20
R18659 T27161 T27162 arg1Of zVAD.fmk,or
R18660 T27162 T27158 arg2Of or,","
R18661 T27166 T27162 arg2Of TNFα,or
R18662 T27166 T27163 arg1Of TNFα,10
R18663 T27166 T27164 arg1Of TNFα,ng/ml
R18664 T27166 T27165 arg1Of TNFα,mouse
R18665 T27167 T27168 arg1Of Cells,were
R18666 T27167 T27169 arg2Of Cells,harvested
R18667 T27169 T27168 arg2Of harvested,were
R18668 T27169 T27170 arg1Of harvested,in
R18669 T27172 T27170 arg2Of buffer,in
R18670 T27172 T27171 arg1Of buffer,1×RIPA
R18671 T27172 T27173 arg1Of buffer,(
R18672 T27172 T27177 arg2Of buffer,supplemented
R18673 T27175 T27173 arg2Of Signaling,(
R18674 T27175 T27174 arg1Of Signaling,Cell
R18675 T27176 T27173 arg3Of ),(
R18676 T27177 T27178 arg1Of supplemented,with
R18677 T27181 T27178 arg2Of phenylmethanesulfonylfluoride,with
R18678 T27181 T27179 arg1Of phenylmethanesulfonylfluoride,50
R18679 T27181 T27180 arg1Of phenylmethanesulfonylfluoride,µg/ml
R18680 T27184 T27182 arg2Of sonication,After
R18681 T27184 T27183 arg1Of sonication,brief
R18682 T27187 T27186 arg1Of lysates,cell
R18683 T27187 T27188 arg1Of lysates,were
R18684 T27187 T27189 arg2Of lysates,spun
R18685 T27189 T27182 arg1Of spun,After
R18686 T27189 T27185 arg1Of spun,","
R18687 T27189 T27188 arg2Of spun,were
R18688 T27189 T27190 arg1Of spun,down
R18689 T27189 T27191 arg1Of spun,for
R18690 T27189 T27194 arg1Of spun,at
R18691 T27193 T27191 arg2Of min,for
R18692 T27193 T27192 arg1Of min,15
R18693 T27195 T27194 arg2Of "14,000×rpm",at
R18694 T27197 T27196 arg1Of concentrations,Protein
R18695 T27197 T27198 arg1Of concentrations,were
R18696 T27197 T27199 arg2Of concentrations,measured
R18697 T27199 T27198 arg2Of measured,were
R18698 T27200 T27199 arg3Of using,measured
R18699 T27200 T27207 arg1Of using,(
R18700 T27202 T27203 arg1Of Pierce,660
R18701 T27206 T27200 arg2Of Reagent,using
R18702 T27206 T27201 arg1Of Reagent,the
R18703 T27206 T27202 arg1Of Reagent,Pierce
R18704 T27206 T27204 arg1Of Reagent,nm
R18705 T27206 T27205 arg1Of Reagent,Assay
R18706 T27208 T27207 arg2Of Pierce,(
R18707 T27209 T27207 arg3Of ),(
R18708 T27211 T27210 arg1Of amounts,Equal
R18709 T27211 T27212 arg1Of amounts,of
R18710 T27211 T27214 arg1Of amounts,were
R18711 T27211 T27215 arg2Of amounts,boiled
R18712 T27213 T27212 arg2Of proteins,of
R18713 T27215 T27214 arg2Of boiled,were
R18714 T27215 T27216 arg1Of boiled,for
R18715 T27218 T27216 arg2Of min,for
R18716 T27218 T27217 arg1Of min,5
R18717 T27218 T27219 arg1Of min,at
R18718 T27221 T27219 arg2Of Western,at
R18719 T27221 T27220 arg1Of Western,95°C.
R18720 T27222 T27223 arg1Of blotting,was
R18721 T27222 T27224 arg2Of blotting,performed
R18722 T27224 T27214 modOf performed,were
R18723 T27224 T27223 arg2Of performed,was
R18724 T27224 T27225 arg1Of performed,according
R18725 T27226 T27225 arg2Of to,according
R18726 T27228 T27226 arg2Of protocols,to
R18727 T27228 T27227 arg1Of protocols,standard
R18728 T27232 T27231 arg1Of gels,SDS-PAGE
R18729 T27232 T27233 arg1Of gels,were
R18730 T27232 T27234 arg2Of gels,transferred
R18731 T27232 T27239 arg1Of gels,blocked
R18732 T27234 T27233 arg2Of transferred,were
R18733 T27234 T27235 arg1Of transferred,to
R18734 T27234 T27238 arg1Of transferred,","
R18735 T27237 T27235 arg2Of membrane,to
R18736 T27237 T27236 arg1Of membrane,PVDF
R18737 T27238 T27229 arg1Of ",",Briefly
R18738 T27238 T27230 arg1Of ",",","
R18739 T27238 T27256 arg1Of ",",for
R18740 T27239 T27238 arg2Of blocked,","
R18741 T27239 T27240 arg1Of blocked,in
R18742 T27242 T27240 arg2Of %,in
R18743 T27242 T27241 arg1Of %,3
R18744 T27243 T27244 arg1Of milk,or
R18745 T27244 T27239 arg2Of or,blocked
R18746 T27245 T27246 arg1Of 5,%
R18747 T27249 T27244 arg2Of albumin,or
R18748 T27249 T27245 arg1Of albumin,5
R18749 T27249 T27247 arg1Of albumin,bovine
R18750 T27249 T27248 arg1Of albumin,serum
R18751 T27249 T27250 arg1Of albumin,(
R18752 T27249 T27253 arg1Of albumin,in
R18753 T27251 T27250 arg2Of BSA,(
R18754 T27252 T27250 arg3Of ),(
R18755 T27255 T27253 arg2Of buffer,in
R18756 T27255 T27254 arg1Of buffer,TBST
R18757 T27258 T27256 arg2Of min,for
R18758 T27258 T27257 arg1Of min,30
R18759 T27258 T27259 arg1Of min,at
R18760 T27261 T27259 arg2Of temperature,at
R18761 T27261 T27260 arg1Of temperature,room
R18762 T27263 T27262 arg1Of antibodies,Primary
R18763 T27263 T27264 arg1Of antibodies,were
R18764 T27263 T27265 arg2Of antibodies,incubated
R18765 T27265 T27264 arg2Of incubated,were
R18766 T27265 T27266 arg1Of incubated,in
R18767 T27265 T27270 arg1Of incubated,overnight
R18768 T27267 T27268 arg1Of 5,%
R18769 T27269 T27266 arg2Of BSA/TBST,in
R18770 T27269 T27267 arg1Of BSA/TBST,5
R18771 T27270 T27275 modOf overnight,were
R18772 T27273 T27272 arg1Of Secondary,4°C.
R18773 T27274 T27273 arg1Of antibodies,Secondary
R18774 T27274 T27275 arg1Of antibodies,were
R18775 T27274 T27276 arg2Of antibodies,incubated
R18776 T27276 T27271 arg1Of incubated,at
R18777 T27276 T27275 arg2Of incubated,were
R18778 T27276 T27277 arg1Of incubated,in
R18779 T27276 T27279 arg1Of incubated,for
R18780 T27276 T27282 arg1Of incubated,at
R18781 T27278 T27277 arg2Of TBST,in
R18782 T27281 T27279 arg2Of min,for
R18783 T27281 T27280 arg1Of min,30
R18784 T27284 T27282 arg2Of temperature,at
R18785 T27284 T27283 arg1Of temperature,room
R18786 T27287 T27286 arg2Of Millipore,(
R18787 T27288 T27286 arg3Of ),(
R18788 T27290 T27285 arg1Of reagents,Luminata
R18789 T27290 T27286 arg1Of reagents,(
R18790 T27290 T27289 arg1Of reagents,ECL
R18791 T27290 T27291 arg1Of reagents,were
R18792 T27290 T27292 arg2Of reagents,used
R18793 T27292 T27291 arg2Of used,were
R18794 T27294 T27292 arg3Of develop,used
R18795 T27294 T27293 arg1Of develop,to
R18796 T27296 T27294 arg2Of signals,develop
R18797 T27296 T27295 arg1Of signals,the
R18798 T27299 T27297 arg2Of cases,In
R18799 T27299 T27298 arg1Of cases,some
R18800 T27301 T27302 arg1Of membranes,were
R18801 T27301 T27303 arg2Of membranes,stripped
R18802 T27301 T27313 arg2Of membranes,reprobed
R18803 T27303 T27312 arg1Of stripped,and
R18804 T27304 T27303 arg3Of using,stripped
R18805 T27305 T27304 arg2Of OneMinute,using
R18806 T27305 T27306 arg1Of OneMinute,stripping
R18807 T27307 T27306 arg2Of buffer,stripping
R18808 T27307 T27308 arg1Of buffer,(
R18809 T27310 T27308 arg2Of Biosciences,(
R18810 T27310 T27309 arg1Of Biosciences,GM
R18811 T27311 T27308 arg3Of ),(
R18812 T27312 T27297 arg1Of and,In
R18813 T27312 T27300 arg1Of and,","
R18814 T27312 T27302 arg2Of and,were
R18815 T27313 T27312 arg2Of reprobed,and
R18816 T27313 T27314 arg1Of reprobed,with
R18817 T27316 T27314 arg2Of antibodies,with
R18818 T27316 T27315 arg1Of antibodies,new
R19061 T27618 T27619 arg1Of Cells,were
R19062 T27618 T27620 arg2Of Cells,treated
R19063 T27620 T27619 arg2Of treated,were
R19064 T27620 T27621 arg1Of treated,as
R19065 T27622 T27621 arg2Of described,as
R19066 T27622 T27623 arg1Of described,for
R19067 T27625 T27623 arg2Of blots,for
R19068 T27625 T27624 arg1Of blots,Western
R19069 T27627 T27626 arg1Of RNA,Total
R19070 T27627 T27628 arg1Of RNA,was
R19071 T27627 T27629 arg2Of RNA,isolated
R19072 T27629 T27628 arg2Of isolated,was
R19073 T27630 T27629 arg3Of using,isolated
R19074 T27633 T27630 arg2Of kit,using
R19075 T27633 T27631 arg1Of kit,ZR
R19076 T27633 T27632 arg1Of kit,Miniprep
R19077 T27633 T27634 arg1Of kit,(
R19078 T27636 T27634 arg2Of Research,(
R19079 T27636 T27635 arg1Of Research,Zymo
R19080 T27637 T27634 arg3Of ),(
R19081 T27639 T27638 arg1Of µg,1
R19082 T27639 T27640 arg1Of µg,of
R19083 T27639 T27642 arg1Of µg,was
R19084 T27639 T27643 arg2Of µg,converted
R19085 T27641 T27640 arg2Of RNA,of
R19086 T27643 T27642 arg2Of converted,was
R19087 T27643 T27644 arg1Of converted,to
R19088 T27645 T27644 arg2Of cDNA,to
R19089 T27645 T27646 arg1Of cDNA,using
R19090 T27648 T27646 arg2Of primers,using
R19091 T27648 T27647 arg1Of primers,random
R19092 T27648 T27649 arg1Of primers,(
R19093 T27652 T27649 arg2Of kit,(
R19094 T27652 T27650 arg1Of kit,M-MuLV
R19095 T27652 T27651 arg1Of kit,cDNA
R19096 T27652 T27653 arg1Of kit,","
R19097 T27656 T27653 arg2Of Biolabs,","
R19098 T27656 T27654 arg1Of Biolabs,New
R19099 T27656 T27655 arg1Of Biolabs,England
R19100 T27657 T27649 arg3Of ),(
R19101 T27659 T27658 arg1Of µL,1
R19102 T27659 T27660 arg1Of µL,of
R19103 T27659 T27662 arg1Of µL,was
R19104 T27659 T27663 arg2Of µL,used
R19105 T27661 T27660 arg2Of cDNA,of
R19106 T27663 T27662 arg2Of used,was
R19107 T27663 T27664 arg1Of used,with
R19108 T27667 T27664 arg2Of primers,with
R19109 T27667 T27665 arg1Of primers,500
R19110 T27667 T27666 arg1Of primers,pM
R19111 T27667 T27668 arg1Of primers,in
R19112 T27670 T27668 arg2Of reactions,in
R19113 T27670 T27669 arg1Of reactions,qPCR
R19114 T27671 T27672 arg1Of Reactions,were
R19115 T27673 T27672 arg2Of performs,were
R19116 T27673 T27674 arg1Of performs,using
R19117 T27677 T27674 arg2Of mix,using
R19118 T27677 T27675 arg1Of mix,SYBRGreen
R19119 T27677 T27676 arg1Of mix,2×Master
R19120 T27677 T27678 arg1Of mix,(
R19121 T27677 T27681 arg1Of mix,in
R19122 T27679 T27678 arg2Of SABiosciences,(
R19123 T27680 T27678 arg3Of ),(
R19124 T27683 T27681 arg2Of LightCycler480,in
R19125 T27683 T27682 arg1Of LightCycler480,a
R19126 T27683 T27684 arg1Of LightCycler480,(
R19127 T27685 T27684 arg2Of Roche,(
R19128 T27686 T27684 arg3Of ),(
R19206 T27785 T27784 arg1Of Infection,Stable
R19207 T27785 T27786 arg1Of Infection,of
R19208 T27787 T27786 arg2Of Myr-Akt1,of
R19209 T27789 T27788 arg1Of generate,To
R19210 T27791 T27789 arg2Of retroviruses,generate
R19211 T27791 T27790 arg1Of retroviruses,MSCV
R19212 T27794 T27789 arg1Of cells,generate
R19213 T27794 T27793 arg1Of cells,HEK293FT
R19214 T27794 T27795 arg1Of cells,(
R19215 T27794 T27798 arg1Of cells,were
R19216 T27794 T27799 arg2Of cells,transfected
R19217 T27796 T27795 arg2Of Invitrogen,(
R19218 T27797 T27795 arg3Of ),(
R19219 T27799 T27798 arg2Of transfected,were
R19220 T27799 T27800 arg1Of transfected,with
R19221 T27799 T27806 arg1Of transfected,and
R19222 T27802 T27800 arg2Of µg,with
R19223 T27802 T27801 arg1Of µg,2
R19224 T27802 T27803 arg1Of µg,of
R19225 T27805 T27803 arg2Of DNA,of
R19226 T27805 T27804 arg1Of DNA,viral
R19227 T27806 T27788 modOf and,To
R19228 T27806 T27792 arg1Of and,","
R19229 T27808 T27807 arg1Of µg,1
R19230 T27808 T27809 arg1Of µg,of
R19231 T27808 T27815 arg1Of µg,in
R19232 T27808 T27818 arg1Of µg,plates
R19233 T27808 T27819 arg1Of µg,using
R19234 T27810 T27811 arg1Of gal/pol,and
R19235 T27813 T27811 arg2Of accessory,and
R19236 T27813 T27812 arg1Of accessory,VSV-G
R19237 T27814 T27809 arg2Of plasmids,of
R19238 T27814 T27810 arg1Of plasmids,gal/pol
R19239 T27814 T27813 arg1Of plasmids,accessory
R19240 T27816 T27815 arg2Of 6,in
R19241 T27818 T27806 arg2Of plates,and
R19242 T27818 T27817 arg1Of plates,well
R19243 T27819 T27818 arg2Of using,plates
R19244 T27822 T27819 arg2Of reagent,using
R19245 T27822 T27820 arg1Of reagent,GenJet
R19246 T27822 T27821 arg1Of reagent,transfection
R19247 T27822 T27823 arg1Of reagent,(
R19248 T27825 T27823 arg2Of Labs,(
R19249 T27825 T27824 arg1Of Labs,Signagen
R19250 T27826 T27823 arg3Of ),(
R19251 T27828 T27827 arg1Of media,Virus-containing
R19252 T27828 T27829 arg1Of media,was
R19253 T27828 T27830 arg2Of media,collected
R19254 T27828 T27835 arg2Of media,filtered
R19255 T27828 T27841 arg2Of media,applied
R19256 T27830 T27832 arg1Of collected,hr
R19257 T27830 T27833 arg1Of collected,later
R19258 T27830 T27834 arg1Of collected,","
R19259 T27832 T27831 arg1Of hr,72
R19260 T27834 T27840 arg1Of ",",and
R19261 T27835 T27834 arg2Of filtered,","
R19262 T27835 T27836 arg1Of filtered,through
R19263 T27837 T27838 arg1Of 0.45,µm
R19264 T27839 T27836 arg2Of filter,through
R19265 T27839 T27837 arg1Of filter,0.45
R19266 T27840 T27829 arg2Of and,was
R19267 T27841 T27840 arg2Of applied,and
R19268 T27841 T27842 arg1Of applied,to
R19269 T27844 T27842 arg2Of cells,to
R19270 T27844 T27843 arg1Of cells,L929
R19271 T27844 T27845 arg1Of cells,with
R19272 T27848 T27845 arg2Of polybrene,with
R19273 T27848 T27846 arg1Of polybrene,8
R19274 T27848 T27847 arg1Of polybrene,µg/ml
R19275 T27849 T27850 arg1Of Cells,were
R19276 T27849 T27851 arg2Of Cells,selected
R19277 T27849 T27853 arg2Of Cells,maintained
R19278 T27851 T27852 arg1Of selected,and
R19279 T27852 T27850 arg2Of and,were
R19280 T27852 T27854 arg1Of and,in
R19281 T27853 T27852 arg2Of maintained,and
R19282 T27857 T27854 arg2Of puromycin,in
R19283 T27857 T27855 arg1Of puromycin,10
R19284 T27857 T27856 arg1Of puromycin,µg/ml
R19367 T27976 T27975 arg1Of Assay,ELISAOne
R19368 T27978 T27977 arg1Of assays,ELISAOne
R19369 T27978 T27979 arg1Of assays,(
R19370 T27978 T27986 arg1Of assays,were
R19371 T27978 T27987 arg2Of assays,performed
R19372 T27980 T27981 arg1Of TGRBio,","
R19373 T27981 T27979 arg2Of ",",(
R19374 T27981 T27983 arg1Of ",",","
R19375 T27982 T27981 arg2Of Hindmarsh,","
R19376 T27984 T27983 arg2Of Australia,","
R19377 T27985 T27979 arg3Of ),(
R19378 T27987 T27986 arg2Of performed,were
R19379 T27987 T27988 arg1Of performed,according
R19380 T27989 T27988 arg2Of to,according
R19381 T27991 T27989 arg2Of protocol,to
R19382 T27991 T27990 arg1Of protocol,manufacturer’s
R19383 T27991 T27992 arg1Of protocol,with
R19384 T27995 T27992 arg2Of modifications,with
R19385 T27995 T27993 arg1Of modifications,the
R19386 T27995 T27994 arg1Of modifications,following
R19387 T27997 T27996 arg1Of lysates,Cell
R19388 T27997 T27998 arg1Of lysates,were
R19389 T27997 T27999 arg2Of lysates,prepared
R19390 T27999 T27998 arg2Of prepared,were
R19391 T27999 T28000 arg1Of prepared,in
R19392 T27999 T28003 arg1Of prepared,as
R19393 T28002 T28000 arg2Of buffer,in
R19394 T28002 T28001 arg1Of buffer,RIPA
R19395 T28004 T28003 arg2Of described,as
R19396 T28004 T28005 arg1Of described,for
R19397 T28007 T28005 arg2Of blots,for
R19398 T28007 T28006 arg1Of blots,Western
R19399 T28009 T28008 arg1Of microliters,Five
R19400 T28009 T28010 arg1Of microliters,of
R19401 T28009 T28012 arg1Of microliters,were
R19402 T28009 T28013 arg2Of microliters,diluted
R19403 T28011 T28010 arg2Of samples,of
R19404 T28013 T28012 arg2Of diluted,were
R19405 T28013 T28014 arg1Of diluted,in
R19406 T28016 T28014 arg2Of µL,in
R19407 T28016 T28015 arg1Of µL,45
R19408 T28016 T28017 arg1Of µL,of
R19409 T28020 T28017 arg2Of buffer,of
R19410 T28020 T28018 arg1Of buffer,ELISAOne
R19411 T28020 T28019 arg1Of buffer,lysis
R19412 T28020 T28021 arg1Of buffer,prior
R19413 T28021 T28022 arg1Of prior,to
R19414 T28023 T28022 arg2Of analysis,to
R19415 T28025 T28024 arg1Of antibodies,Primary
R19416 T28025 T28026 arg1Of antibodies,to
R19417 T28025 T28030 arg1Of antibodies,were
R19418 T28025 T28031 arg2Of antibodies,incubated
R19419 T28027 T28028 arg1Of phopsho-Thr308,and
R19420 T28028 T28026 arg2Of and,to
R19421 T28029 T28028 arg2Of phopsho-Ser473,and
R19422 T28031 T28030 arg2Of incubated,were
R19423 T28031 T28032 arg1Of incubated,with
R19424 T28034 T28032 arg2Of samples,with
R19425 T28034 T28033 arg1Of samples,the
R19426 T28034 T28035 arg1Of samples,for
R19427 T28037 T28035 arg2Of hr,for
R19428 T28037 T28036 arg1Of hr,2
R19429 T28037 T28038 arg1Of hr,at
R19430 T28040 T28038 arg2Of temperature,at
R19431 T28040 T28039 arg1Of temperature,room
R19432 T28042 T28041 arg1Of antibody,Primary
R19433 T28042 T28043 arg1Of antibody,to
R19434 T28042 T28045 arg1Of antibody,was
R19435 T28042 T28046 arg2Of antibody,incubated
R19436 T28044 T28043 arg2Of pan-Akt,to
R19437 T28046 T28045 arg2Of incubated,was
R19438 T28046 T28047 arg1Of incubated,overnight
R19439 T28046 T28048 arg1Of incubated,at
R19440 T28046 T28051 arg1Of incubated,for
R19441 T28050 T28048 arg2Of Signals,at
R19442 T28050 T28049 arg1Of Signals,4°C.
R19443 T28052 T28053 arg1Of phospho-antibodies,were
R19444 T28052 T28054 arg2Of phospho-antibodies,normalized
R19445 T28054 T28051 arg2Of normalized,for
R19446 T28054 T28053 arg2Of normalized,were
R19447 T28054 T28055 arg1Of normalized,based
R19448 T28056 T28055 arg2Of on,based
R19449 T28058 T28056 arg2Of values,on
R19450 T28058 T28057 arg1Of values,pan-Akt
R19542 T28184 T28183 arg1Of ELISA,TNFα
R19543 T28189 T28185 arg1Of assays,Mouse
R19544 T28189 T28186 arg1Of assays,TNFα
R19545 T28189 T28187 arg1Of assays,Quantikine
R19546 T28189 T28188 arg1Of assays,ELISA
R19547 T28189 T28190 arg1Of assays,(
R19548 T28189 T28196 arg1Of assays,were
R19549 T28189 T28197 arg2Of assays,performed
R19550 T28194 T28190 arg2Of Systems,(
R19551 T28194 T28191 arg1Of Systems,R
R19552 T28194 T28192 arg1Of Systems,&
R19553 T28194 T28193 arg1Of Systems,D
R19554 T28195 T28190 arg3Of ),(
R19555 T28197 T28196 arg2Of performed,were
R19556 T28197 T28198 arg1Of performed,according
R19557 T28199 T28198 arg2Of to,according
R19558 T28201 T28199 arg2Of descriptions,to
R19559 T28201 T28200 arg1Of descriptions,manufacturer’s
R19560 T28203 T28202 arg1Of lysates,Cell
R19561 T28203 T28204 arg1Of lysates,were
R19562 T28203 T28205 arg2Of lysates,prepared
R19563 T28203 T28211 arg2Of lysates,treated
R19564 T28205 T28206 arg1Of prepared,from
R19565 T28205 T28210 arg1Of prepared,and
R19566 T28208 T28206 arg2Of cells,from
R19567 T28208 T28207 arg1Of cells,3×106
R19568 T28208 T28209 arg2Of cells,plated
R19569 T28210 T28204 arg2Of and,were
R19570 T28211 T28210 arg2Of treated,and
R19571 T28211 T28212 arg1Of treated,in
R19572 T28216 T28212 arg2Of dish,in
R19573 T28216 T28213 arg1Of dish,a
R19574 T28216 T28214 arg1Of dish,10
R19575 T28216 T28215 arg1Of dish,cm2
R19612 T28297 T28296 arg1Of vitro,In
R19613 T28300 T28297 arg1Of Assay,vitro
R19614 T28300 T28298 arg1Of Assay,Akt
R19615 T28300 T28299 arg1Of Assay,Kinase
R19616 T28303 T28301 arg1Of activity,Akt
R19617 T28303 T28302 arg1Of activity,kinase
R19618 T28303 T28304 arg1Of activity,was
R19619 T28303 T28305 arg2Of activity,measured
R19620 T28305 T28304 arg2Of measured,was
R19621 T28306 T28305 arg3Of using,measured
R19622 T28311 T28306 arg2Of kit,using
R19623 T28311 T28307 arg1Of kit,the
R19624 T28311 T28308 arg1Of kit,Akt
R19625 T28311 T28309 arg1Of kit,kinase
R19626 T28311 T28310 arg1Of kit,assay
R19627 T28311 T28312 arg1Of kit,(
R19628 T28311 T28315 arg1Of kit,from
R19629 T28313 T28312 arg2Of nonradioactive,(
R19630 T28314 T28312 arg3Of ),(
R19631 T28318 T28315 arg2Of Technology.,from
R19632 T28318 T28316 arg1Of Technology.,Cell
R19633 T28318 T28317 arg1Of Technology.,Signaling
R19634 T28318 T28319 arg1Of Technology.,In
R19635 T28320 T28319 arg2Of brief,In
R19636 T28322 T28323 arg1Of Myr-Akt,was
R19637 T28322 T28324 arg2Of Myr-Akt,immunoprecipitated
R19638 T28324 T28304 modOf immunoprecipitated,was
R19639 T28324 T28321 arg1Of immunoprecipitated,","
R19640 T28324 T28323 arg2Of immunoprecipitated,was
R19641 T28324 T28325 arg1Of immunoprecipitated,from
R19642 T28327 T28325 arg2Of cells,from
R19643 T28327 T28326 arg1Of cells,L929
R19644 T28327 T28328 arg1Of cells,using
R19645 T28332 T28328 arg2Of beads,using
R19646 T28332 T28329 arg1Of beads,anti-FLAG
R19647 T28332 T28330 arg1Of beads,M2
R19648 T28332 T28331 arg1Of beads,magnetic
R19649 T28332 T28333 arg1Of beads,(
R19650 T28334 T28333 arg2Of Sigma,(
R19651 T28335 T28333 arg3Of ),(
R19652 T28338 T28337 arg1Of vitro,in
R19653 T28339 T28336 arg1Of assay,The
R19654 T28339 T28338 arg1Of assay,vitro
R19655 T28339 T28340 arg1Of assay,was
R19656 T28339 T28341 arg2Of assay,performed
R19657 T28341 T28340 arg2Of performed,was
R19658 T28341 T28342 arg1Of performed,in
R19659 T28344 T28342 arg2Of presence,in
R19660 T28344 T28343 arg1Of presence,the
R19661 T28344 T28345 arg1Of presence,of
R19662 T28350 T28345 arg2Of substrate,of
R19663 T28350 T28346 arg1Of substrate,a
R19664 T28350 T28347 arg1Of substrate,GSK
R19665 T28350 T28348 arg1Of substrate,fusion
R19666 T28350 T28349 arg1Of substrate,protein
R19667 T28351 T28352 arg1Of Phosphorylation,of
R19668 T28351 T28357 arg1Of Phosphorylation,was
R19669 T28351 T28358 arg2Of Phosphorylation,visualized
R19670 T28356 T28352 arg2Of protein,of
R19671 T28356 T28353 arg1Of protein,the
R19672 T28356 T28354 arg1Of protein,GSK
R19673 T28356 T28355 arg1Of protein,fusion
R19674 T28358 T28357 arg2Of visualized,was
R19675 T28361 T28358 arg1Of blot,visualized
R19676 T28361 T28359 arg2Of blot,by
R19677 T28361 T28360 arg1Of blot,western
R17904 T26209 T26208 arg1Of Lines,Cell
R17905 T26214 T26210 arg1Of cells,L929
R17906 T26214 T26211 arg1Of cells,and
R17907 T26214 T26212 arg1Of cells,FADD-deficient
R17908 T26214 T26213 arg1Of cells,Jurkat
R17909 T26214 T26215 arg1Of cells,were
R17910 T26214 T26216 arg2Of cells,obtained
R17911 T26216 T26215 arg2Of obtained,were
R17912 T26216 T26217 arg1Of obtained,from
R17913 T26218 T26217 arg2Of ATCC,from
R17914 T26220 T26219 arg1Of fibroblasts,Lung
R17915 T26220 T26221 arg1Of fibroblasts,were
R17917 T26224 T26221 arg2Of gift,were
R17918 T26224 T26222 arg1Of gift,a
R17919 T26224 T26223 arg1Of gift,generous
R17920 T26224 T26225 arg1Of gift,of
R17921 T26228 T26225 arg2Of Tsichlis,of
R17922 T26228 T26226 arg1Of Tsichlis,Dr.
R17923 T26228 T26227 arg1Of Tsichlis,Philip
R17924 T26228 T26229 arg1Of Tsichlis,(
R17925 T26231 T26229 arg2Of University,(

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T26736 4532-4536 Protein denotes TNFα
T26735 4333-4338 Protein denotes Mapk9
T26734 4306-4311 Protein denotes Mapk8
T26733 4243-4247 Protein denotes PDK1
T26732 4191-4195 Protein denotes Akt3
T26731 4165-4169 Protein denotes Akt2
T26730 4139-4143 Protein denotes Akt1
T25594 1186-1199 Protein denotes Myr-Akt1-FLAG
T25593 1119-1127 Protein denotes Myr-Akt1
T26002 2671-2674 Protein denotes 18S
T26001 2623-2626 Protein denotes 18S
T26000 2573-2577 Protein denotes TNFα
T25999 2522-2526 Protein denotes TNFα
T25998 2432-2435 Protein denotes 18S
T25997 2347-2351 Protein denotes TNFα
T28175 7226-7230 Protein denotes TNFα
T28174 7209-7213 Protein denotes TNFα
T28284 7557-7564 Protein denotes Myr-Akt
T27078 4936-4940 Protein denotes TNFα
T27077 4777-4781 Protein denotes TNFα
T26200 2733-2737 Protein denotes FADD
T25141 1012-1017 Protein denotes IGF-1
T25140 972-975 Protein denotes EGF
T25139 955-959 Protein denotes bFGF
T25138 932-936 Protein denotes TNFα
T27779 6226-6234 Protein denotes Myr-Akt1
T25595 1331-1339 Protein denotes Myr-Akt1

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T28375 7561-7564 http://www.uniprot.org/uniprot/Q9Y243 denotes Akt
T28374 7477-7480 http://www.uniprot.org/uniprot/Q9Y243 denotes Akt
T28373 7434-7437 http://www.uniprot.org/uniprot/Q9Y243 denotes Akt
T28372 7417-7420 http://www.uniprot.org/uniprot/Q9Y243 denotes Akt
T28371 7561-7564 http://www.uniprot.org/uniprot/P31751 denotes Akt
T28370 7477-7480 http://www.uniprot.org/uniprot/P31751 denotes Akt
T28369 7434-7437 http://www.uniprot.org/uniprot/P31751 denotes Akt
T28368 7417-7420 http://www.uniprot.org/uniprot/P31751 denotes Akt
T28367 7561-7564 http://www.uniprot.org/uniprot/P31749 denotes Akt
T28366 7477-7480 http://www.uniprot.org/uniprot/P31749 denotes Akt
T28365 7434-7437 http://www.uniprot.org/uniprot/P31749 denotes Akt
T28364 7417-7420 http://www.uniprot.org/uniprot/P31749 denotes Akt
T28220 7226-7230 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T28219 7209-7213 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T27320 4936-4940 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T27319 4777-4781 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T26070 2573-2577 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T26069 2522-2526 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T26068 2347-2351 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T26874 4532-4536 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T26873 4217-4221 http://www.uniprot.org/uniprot/P42345 denotes mTOR
T25742 1729-1734 http://www.uniprot.org/uniprot/P05412 denotes c-Jun
T25258 955-959 http://www.uniprot.org/uniprot/Q788Q8 denotes bFGF
T25257 932-936 http://www.uniprot.org/uniprot/P01375 denotes TNFα
T25256 246-249 http://www.uniprot.org/uniprot/Q9Y243 denotes Akt
T25255 246-249 http://www.uniprot.org/uniprot/P31751 denotes Akt
T25254 246-249 http://www.uniprot.org/uniprot/P31749 denotes Akt
T25745 2230-2234 http://www.uniprot.org/uniprot/P42345 denotes mTOR
T25744 2200-2206 http://www.uniprot.org/uniprot/Q13541 denotes 4E-BP1
T25743 1758-1763 http://www.uniprot.org/uniprot/P05412 denotes c-Jun

UBERON-AE

Id Subject Object Predicate Lexical cue
T27062 5411-5415 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T27061 5429-5434 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27060 4838-4843 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T27059 4804-4809 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T26190 3098-3103 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T26189 3172-3176 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T26188 2786-2790 http://purl.obolibrary.org/obo/UBERON_0002048 denotes Lung

GO-BP

Id Subject Object Predicate Lexical cue
T27967 7136-7143 http://purl.obolibrary.org/obo/GO_0023052 denotes Signals
T27966 6926-6931 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T28288 7731-7746 http://purl.obolibrary.org/obo/GO_0016310 denotes Phosphorylation
T28287 7754-7757 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T28286 7701-7704 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T28285 7438-7453 http://purl.obolibrary.org/obo/GO_0016301 denotes kinase activity
T27080 5697-5704 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T27079 4887-4893 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T26737 4243-4247 http://purl.obolibrary.org/obo/GO_0004740 denotes PDK1
T25729 2260-2264 http://purl.obolibrary.org/obo/GO_0004740 denotes PDK1
T25728 1985-1988 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T25727 1700-1704 http://purl.obolibrary.org/obo/GO_0016909 denotes SAPK
T25726 1705-1708 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T25725 1660-1663 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T26482 3822-3833 http://purl.obolibrary.org/obo/GO_0097528 denotes necroptosis
T26481 3822-3833 http://purl.obolibrary.org/obo/GO_0070266 denotes necroptosis
T25142 396-399 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK

GO-MF

Id Subject Object Predicate Lexical cue
T27969 7156-7166 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27968 6966-6976 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T28291 7754-7757 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T28290 7701-7704 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T28289 7438-7453 http://purl.obolibrary.org/obo/GO_0016301 denotes kinase activity
T27083 5817-5827 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27082 5572-5582 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T27081 5504-5514 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T26738 4243-4247 http://purl.obolibrary.org/obo/GO_0004740 denotes PDK1
T25737 2260-2264 http://purl.obolibrary.org/obo/GO_0004740 denotes PDK1
T25736 2150-2167 http://purl.obolibrary.org/obo/GO_0003735 denotes Ribosomal Protein
T25735 2084-2101 http://purl.obolibrary.org/obo/GO_0003735 denotes Ribosomal Protein
T25734 1985-1988 http://purl.obolibrary.org/obo/GO_0050321 denotes GSK
T25733 1700-1704 http://purl.obolibrary.org/obo/GO_0016909 denotes SAPK
T25732 1705-1708 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T25731 1660-1663 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T25730 1406-1416 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T25145 988-992 http://purl.obolibrary.org/obo/GO_0005161 denotes PDGF
T25144 972-975 http://purl.obolibrary.org/obo/GO_0005154 denotes EGF
T25143 396-399 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK

GO-CC

Id Subject Object Predicate Lexical cue
T28293 7598-7603 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28292 7520-7524 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T27781 6553-6558 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27780 6275-6280 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27096 5817-5827 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27095 5572-5582 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27094 5504-5514 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27093 5817-5827 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27092 5572-5582 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27091 5504-5514 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27090 5721-5730 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T27089 5387-5395 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T27088 5079-5083 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T27087 4827-4832 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27086 4717-4722 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27085 4662-4667 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T27084 4642-4647 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T25740 2281-2285 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T25739 1406-1416 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T25738 1406-1416 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T28178 7364-7376 http://purl.obolibrary.org/obo/GO_0009504 denotes cells plated
T28177 7364-7369 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28176 7326-7330 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T27974 7156-7166 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27973 6966-6976 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T27972 7156-7166 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27971 6966-6976 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T27970 6790-6794 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T26740 4578-4582 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T26739 4496-4501 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26488 3905-3910 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26487 3796-3801 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26486 3751-3756 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26485 3629-3633 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T26484 3579-3583 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T26483 3405-3409 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T26205 3297-3302 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26204 2913-2918 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26203 2887-2892 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26202 2755-2760 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T26201 2713-2717 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T25147 1039-1043 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T25146 742-746 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell

sentences

Id Subject Object Predicate Lexical cue
T27965 7076-7207 Sentence denotes Primary antibody to pan-Akt was incubated overnight at 4°C. Signals for phospho-antibodies were normalized based on pan-Akt values.
T27964 6958-7075 Sentence denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473 were incubated with the samples for 2 hr at room temperature.
T27963 6864-6957 Sentence denotes Five microliters of samples were diluted in 45 µL of ELISAOne lysis buffer prior to analysis.
T27962 6790-6863 Sentence denotes Cell lysates were prepared in RIPA buffer as described for Western blots.
T27961 6657-6789 Sentence denotes ELISAOne assays (TGRBio, Hindmarsh, Australia) were performed according to manufacturer’s protocol with the following modifications.
T27960 6642-6656 Sentence denotes ELISAOne Assay
T28282 7731-7804 Sentence denotes Phosphorylation of the GSK fusion protein was visualized by western blot.
T28281 7647-7730 Sentence denotes The in vitro assay was performed in the presence of a GSK fusion protein substrate.
T28280 7434-7646 Sentence denotes Akt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology. In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma).
T28279 7408-7433 Sentence denotes In vitro Akt Kinase Assay
T27616 6107-6204 Sentence denotes Reactions were performs using SYBRGreen 2×Master mix (SABiosciences) in a LightCycler480 (Roche).
T27615 6046-6106 Sentence denotes 1 µL of cDNA was used with 500 pM primers in qPCR reactions.
T27614 5951-6045 Sentence denotes 1 µg of RNA was converted to cDNA using random primers (M-MuLV cDNA kit, New England Biolabs).
T27613 5889-5950 Sentence denotes Total RNA was isolated using ZR Miniprep kit (Zymo Research).
T27612 5838-5888 Sentence denotes Cells were treated as described for Western blots.
T27611 5830-5837 Sentence denotes qRT-PCR
T27777 6583-6640 Sentence denotes Cells were selected and maintained in 10 µg/ml puromycin.
T27776 6451-6582 Sentence denotes Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene.
T27775 6235-6450 Sentence denotes To generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs).
T27774 6206-6234 Sentence denotes Stable Infection of Myr-Akt1
T27074 5706-5828 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.
T27073 5638-5705 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T27072 5496-5637 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C. Secondary antibodies were incubated in TBST for 30 min at room temperature.
T27071 5339-5495 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T27070 5218-5338 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C. Western blotting was performed according to standard protocols.
T27069 5133-5217 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T27068 5055-5132 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T27067 4942-5054 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T27066 4783-4941 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27065 4701-4782 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T27064 4609-4700 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T27063 4596-4608 Sentence denotes Western Blot
T26722 4483-4594 Sentence denotes After 72 hr, cells were treated with zVAD.fmk or TNFα for 9 hr (RNA or Western blot) or 24 hr (cell viability).
T26721 4037-4482 Sentence denotes siRNAs were purchased from Dharmacon. Mouse ribosomal S6 protein (L-040893-00 and L-045791-00), mouse Akt1 (L-040709-00), mouse Akt2 (L-040782-00), mouse Akt3 (L-040891-00), mouse mTOR (L-065427-00), mouse PDK1 (L-040658-00), non-coding control (D-001810-10-05), mouse Mapk8 (J-040128-05), mouse Mapk9 (J-040134-05), mouse Jun (L-043776-00). siRNA were transfected using RNAiMAX reagent (Invitrogen), according to manufacturer’s recommendations.
T26720 4021-4036 Sentence denotes siRNA Knockdown
T26193 2786-2871 Sentence denotes Lung fibroblasts were a generous gift of Dr. Philip Tsichlis (Tufts University) [53].
T26192 2724-2785 Sentence denotes L929 and FADD-deficient Jurkat cells were obtained from ATCC.
T26191 2713-2723 Sentence denotes Cell Lines
T25990 2341-2711 Sentence denotes Mouse TNFα: forward 5′-CCCTCACACTCAGATCATCTTCT-3′, reverse 5′-GCTACGACGTGGGCTACAG-3′;mouse 18S: forward 5-′ ATAACAGGTCTGTGATGCCCTTAG-3, reverse 5′-CTAAACCATCCAATCGGTAGTAGC-3′;human TNFα: forward 5′- ATGAGCACTGAAAGCATGATCC-3′, human TNFα: reverse 5′-GAGGGCTGATTAGAGAGAGGTC-3′; human 18S: forward 5′- CAGCCACCCGAGATTGAGCA -3, human 18S: reverse 5′-TAGTAGCGACGGGCGGTGTG-3′.
T25989 2328-2340 Sentence denotes QPCR Primers
T25724 1392-2326 Sentence denotes The following antibodies were used: phospho-Akt (Thr308) (clone C31E5E) rabbit mAb, phospho-Akt (Ser473) (clone D9E) XP rabbit mAb, Akt (pan) (clone C67E7) rabbit mAb, Akt1 (clone C73H10) rabbit mAb, Akt2 (clone D6G4) rabbit mAb, Akt3 (clone 62A8) rabbit mAb, phospho-JNK (Thr183/Tyr185) (81E11) rabbit mAb, SAPK/JNK rabbit pAb, phospho-c-Jun (Ser63) II rabbit pAb, c-Jun (60A8) rabbit mAb, α-tubulin (clone DM1A) mouse mAb, phospho-FoxO1 (Thr24)/FoxO3a (Thr32) rabbit pAb, FoxO1 (L27) rabbit pAb, phospho-FoxO4 (Ser193) rabbit pAb, FoxO4 rabbit pAb, phospho-MDM2 (Ser166) rabbit pAb, phospho-GSK-3α/β (Ser21/9) rabbit pAb, phospho-p70 S6 Kinase (Thr389) (clone 108D2) rabbit mAb, phospho-S6 Ribosomal Protein (Ser235/236) (clone D57.2.2E) XP rabbit mAb, S6 Ribosomal Protein (clone 54D2) mouse mAb, phospho-4E-BP1 (Thr37/46) rabbit pAb, mTOR (clone 7C10) rabbit mAb, PDK1 rabbit pAb (all Cell Signaling), MDM2 rabbit pAb (Bioworlde).
T25723 1381-1391 Sentence denotes Antibodies
T26480 3774-4019 Sentence denotes Relative viability of cells, induced to undergo necroptosis and treated with the compound relative to the control compound-treated cells, was determined and plotted to exclude the possible effects of non-specific toxicity of the small molecules.
T26479 3716-3773 Sentence denotes Viability of the control untreated cells was set as 100%.
T26478 3661-3715 Sentence denotes Experiments were performed in duplicate or triplicate.
T26477 3579-3660 Sentence denotes Cell viability was determined using CellTiter-Glo Cell Viability Assay (Promega).
T26476 3432-3578 Sentence denotes Cells were seeded into white clear bottom 96 well plates at the density of 1×104 cells/well and treated as described for western blot experiments.
T26475 3405-3431 Sentence denotes Cell Viability Experiments
T26197 3290-3403 Sentence denotes Jurkat cells were maintained in RPMI1640, supplemented with 10% FetalPlex (Gemini) and 1% antibiotic-antimycotic.
T26196 3162-3289 Sentence denotes The mouse lung fibroblast media was additionally supplemented with L-glutamine, non-essential amino acids, and sodium pyruvate.
T26195 3033-3161 Sentence denotes Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic mixture (Invitrogen).
T26194 2872-3032 Sentence denotes J774A.1 (ATCC) cells and RAW264.7 (ATCC) cells were generous gifts of Junying Yuan (Harvard University) and Alexander Poltorak (Tufts University), respectively.
T25133 847-1102 Sentence denotes Pan-caspase inhibitor zVAD.fmk (20–30 µM) was purchased from Bachem. Human and mouse TNFα (10 ng/ml), human bFGF (25 ng/ml), EGF (50 ng/ml), PDGF-BB (20 ng/ml), and IGF-1 (50 ng/ml) were from Cell Sciences or Peprotech. All other reagents were from Sigma.
T25132 246-846 Sentence denotes Akt inhibitor VIII (10 µM, Calbiochem), MK-2206 (10 µM, Selleck Chem), Triciribine (100 µM, National Cancer Institute), SP600125 (10 µM, Calbiochem), JNK inhibitor VIII (10 µM, Calbiochem), UO126 (10 mM, Cayman Chem), PD169316 (10 µM, Calbiochem), LiCl (10 mM, Sigma), SB216763 (10 µM, Calbiochem), BX912 (10 µM, Axon Med Chem), PF-4706871 (Sigma), rapamycin (100 nM, Santa Cruz), PI-103 (10 µM, Calbiochem), Torin-1 (500 nM, gift of Dr. Nathanael Grey (Harvard Medical School), LY249002 (10 µM, Cell Signaling), PD173074 (2 µM, Cayman Chem), PD166866 (20 µM, Calbiochem), 4EGI-1 (50 µM, Calbiochem).
T25131 119-245 Sentence denotes The following reagents and final concentrations (unless otherwise specified in the text/figures) were used in the experiments:
T25130 46-118 Sentence denotes Necrostatin analogs were synthesized as previously described [23], [24].
T25129 23-45 Sentence denotes Reagents and Chemicals
T28171 7326-7406 Sentence denotes Cell lysates were prepared from 3×106 cells plated and treated in a 10 cm2 dish.
T28170 7220-7325 Sentence denotes Mouse TNFα Quantikine ELISA assays (R&D Systems) were performed according to manufacturer’s descriptions.
T28169 7209-7219 Sentence denotes TNFα ELISA
T25589 1312-1379 Sentence denotes Mutant versions of Myr-Akt1 were generated using the same strategy.
T25588 1186-1311 Sentence denotes Myr-Akt1-FLAG was amplified by PCR and subcloned into the BglII and EcoRI sites of pMSCV-puro retroviral vector (Invitrogen).
T25587 1108-1185 Sentence denotes Cloning of Myr-Akt1, containing c-terminal FLAG tag, has been described [52].
T25586 1104-1107 Sentence denotes DNA
T308 0-21 Sentence denotes Materials and Methods
T309 23-45 Sentence denotes Reagents and Chemicals
T310 46-118 Sentence denotes Necrostatin analogs were synthesized as previously described [23], [24].
T311 119-245 Sentence denotes The following reagents and final concentrations (unless otherwise specified in the text/figures) were used in the experiments:
T312 246-846 Sentence denotes Akt inhibitor VIII (10 µM, Calbiochem), MK-2206 (10 µM, Selleck Chem), Triciribine (100 µM, National Cancer Institute), SP600125 (10 µM, Calbiochem), JNK inhibitor VIII (10 µM, Calbiochem), UO126 (10 mM, Cayman Chem), PD169316 (10 µM, Calbiochem), LiCl (10 mM, Sigma), SB216763 (10 µM, Calbiochem), BX912 (10 µM, Axon Med Chem), PF-4706871 (Sigma), rapamycin (100 nM, Santa Cruz), PI-103 (10 µM, Calbiochem), Torin-1 (500 nM, gift of Dr. Nathanael Grey (Harvard Medical School), LY249002 (10 µM, Cell Signaling), PD173074 (2 µM, Cayman Chem), PD166866 (20 µM, Calbiochem), 4EGI-1 (50 µM, Calbiochem).
T313 847-915 Sentence denotes Pan-caspase inhibitor zVAD.fmk (20–30 µM) was purchased from Bachem.
T314 916-1066 Sentence denotes Human and mouse TNFα (10 ng/ml), human bFGF (25 ng/ml), EGF (50 ng/ml), PDGF-BB (20 ng/ml), and IGF-1 (50 ng/ml) were from Cell Sciences or Peprotech.
T315 1067-1102 Sentence denotes All other reagents were from Sigma.
T316 1104-1107 Sentence denotes DNA
T317 1108-1185 Sentence denotes Cloning of Myr-Akt1, containing c-terminal FLAG tag, has been described [52].
T318 1186-1311 Sentence denotes Myr-Akt1-FLAG was amplified by PCR and subcloned into the BglII and EcoRI sites of pMSCV-puro retroviral vector (Invitrogen).
T319 1312-1379 Sentence denotes Mutant versions of Myr-Akt1 were generated using the same strategy.
T320 1381-1391 Sentence denotes Antibodies
T321 1392-2326 Sentence denotes The following antibodies were used: phospho-Akt (Thr308) (clone C31E5E) rabbit mAb, phospho-Akt (Ser473) (clone D9E) XP rabbit mAb, Akt (pan) (clone C67E7) rabbit mAb, Akt1 (clone C73H10) rabbit mAb, Akt2 (clone D6G4) rabbit mAb, Akt3 (clone 62A8) rabbit mAb, phospho-JNK (Thr183/Tyr185) (81E11) rabbit mAb, SAPK/JNK rabbit pAb, phospho-c-Jun (Ser63) II rabbit pAb, c-Jun (60A8) rabbit mAb, α-tubulin (clone DM1A) mouse mAb, phospho-FoxO1 (Thr24)/FoxO3a (Thr32) rabbit pAb, FoxO1 (L27) rabbit pAb, phospho-FoxO4 (Ser193) rabbit pAb, FoxO4 rabbit pAb, phospho-MDM2 (Ser166) rabbit pAb, phospho-GSK-3α/β (Ser21/9) rabbit pAb, phospho-p70 S6 Kinase (Thr389) (clone 108D2) rabbit mAb, phospho-S6 Ribosomal Protein (Ser235/236) (clone D57.2.2E) XP rabbit mAb, S6 Ribosomal Protein (clone 54D2) mouse mAb, phospho-4E-BP1 (Thr37/46) rabbit pAb, mTOR (clone 7C10) rabbit mAb, PDK1 rabbit pAb (all Cell Signaling), MDM2 rabbit pAb (Bioworlde).
T322 2328-2340 Sentence denotes QPCR Primers
T323 2341-2711 Sentence denotes Mouse TNFα: forward 5′-CCCTCACACTCAGATCATCTTCT-3′, reverse 5′-GCTACGACGTGGGCTACAG-3′;mouse 18S: forward 5-′ ATAACAGGTCTGTGATGCCCTTAG-3, reverse 5′-CTAAACCATCCAATCGGTAGTAGC-3′;human TNFα: forward 5′- ATGAGCACTGAAAGCATGATCC-3′, human TNFα: reverse 5′-GAGGGCTGATTAGAGAGAGGTC-3′; human 18S: forward 5′- CAGCCACCCGAGATTGAGCA -3, human 18S: reverse 5′-TAGTAGCGACGGGCGGTGTG-3′.
T324 2713-2723 Sentence denotes Cell Lines
T325 2724-2785 Sentence denotes L929 and FADD-deficient Jurkat cells were obtained from ATCC.
T326 2786-2871 Sentence denotes Lung fibroblasts were a generous gift of Dr. Philip Tsichlis (Tufts University) [53].
T327 2872-3032 Sentence denotes J774A.1 (ATCC) cells and RAW264.7 (ATCC) cells were generous gifts of Junying Yuan (Harvard University) and Alexander Poltorak (Tufts University), respectively.
T328 3033-3161 Sentence denotes Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic-antimycotic mixture (Invitrogen).
T329 3162-3289 Sentence denotes The mouse lung fibroblast media was additionally supplemented with L-glutamine, non-essential amino acids, and sodium pyruvate.
T330 3290-3403 Sentence denotes Jurkat cells were maintained in RPMI1640, supplemented with 10% FetalPlex (Gemini) and 1% antibiotic-antimycotic.
T331 3405-3431 Sentence denotes Cell Viability Experiments
T332 3432-3578 Sentence denotes Cells were seeded into white clear bottom 96 well plates at the density of 1×104 cells/well and treated as described for western blot experiments.
T333 3579-3660 Sentence denotes Cell viability was determined using CellTiter-Glo Cell Viability Assay (Promega).
T334 3661-3715 Sentence denotes Experiments were performed in duplicate or triplicate.
T335 3716-3773 Sentence denotes Viability of the control untreated cells was set as 100%.
T336 3774-4019 Sentence denotes Relative viability of cells, induced to undergo necroptosis and treated with the compound relative to the control compound-treated cells, was determined and plotted to exclude the possible effects of non-specific toxicity of the small molecules.
T337 4021-4036 Sentence denotes siRNA Knockdown
T338 4037-4074 Sentence denotes siRNAs were purchased from Dharmacon.
T339 4075-4482 Sentence denotes Mouse ribosomal S6 protein (L-040893-00 and L-045791-00), mouse Akt1 (L-040709-00), mouse Akt2 (L-040782-00), mouse Akt3 (L-040891-00), mouse mTOR (L-065427-00), mouse PDK1 (L-040658-00), non-coding control (D-001810-10-05), mouse Mapk8 (J-040128-05), mouse Mapk9 (J-040134-05), mouse Jun (L-043776-00). siRNA were transfected using RNAiMAX reagent (Invitrogen), according to manufacturer’s recommendations.
T340 4483-4594 Sentence denotes After 72 hr, cells were treated with zVAD.fmk or TNFα for 9 hr (RNA or Western blot) or 24 hr (cell viability).
T341 4596-4608 Sentence denotes Western Blot
T342 4609-4700 Sentence denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes.
T343 4701-4782 Sentence denotes After 24–48 hr, cells were stimulated with 30 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T344 4783-4941 Sentence denotes For treatments under serum free conditions, cells were serum starved for 24 hr prior to the addition of growth factors, 20 µM zVAD.fmk or 10 ng/ml mouse TNFα.
T345 4942-5054 Sentence denotes Cells were harvested in 1×RIPA buffer (Cell Signaling) supplemented with 50 µg/ml phenylmethanesulfonylfluoride.
T346 5055-5132 Sentence denotes After brief sonication, cell lysates were spun down for 15 min at 14,000×rpm.
T347 5133-5217 Sentence denotes Protein concentrations were measured using the Pierce 660 nm Assay Reagent (Pierce).
T348 5218-5274 Sentence denotes Equal amounts of proteins were boiled for 5 min at 95°C.
T349 5275-5338 Sentence denotes Western blotting was performed according to standard protocols.
T350 5339-5495 Sentence denotes Briefly, SDS-PAGE gels were transferred to PVDF membrane, blocked in 3% milk or 5% bovine serum albumin (BSA) in TBST buffer for 30 min at room temperature.
T351 5496-5561 Sentence denotes Primary antibodies were incubated in 5%BSA/TBST overnight at 4°C.
T352 5562-5637 Sentence denotes Secondary antibodies were incubated in TBST for 30 min at room temperature.
T353 5638-5705 Sentence denotes Luminata (Millipore) ECL reagents were used to develop the signals.
T354 5706-5828 Sentence denotes In some cases, membranes were stripped using OneMinute stripping buffer (GM Biosciences) and reprobed with new antibodies.
T355 5830-5837 Sentence denotes qRT-PCR
T356 5838-5888 Sentence denotes Cells were treated as described for Western blots.
T357 5889-5950 Sentence denotes Total RNA was isolated using ZR Miniprep kit (Zymo Research).
T358 5951-6045 Sentence denotes 1 µg of RNA was converted to cDNA using random primers (M-MuLV cDNA kit, New England Biolabs).
T359 6046-6106 Sentence denotes 1 µL of cDNA was used with 500 pM primers in qPCR reactions.
T360 6107-6204 Sentence denotes Reactions were performs using SYBRGreen 2×Master mix (SABiosciences) in a LightCycler480 (Roche).
T361 6206-6234 Sentence denotes Stable Infection of Myr-Akt1
T362 6235-6450 Sentence denotes To generate MSCV retroviruses, HEK293FT cells (Invitrogen) were transfected with 2 µg of viral DNA and 1 µg of gal/pol and VSV-G accessory plasmids in 6 well plates using GenJet transfection reagent (Signagen Labs).
T363 6451-6582 Sentence denotes Virus-containing media was collected 72 hr later, filtered through 0.45 µm filter and applied to L929 cells with 8 µg/ml polybrene.
T364 6583-6640 Sentence denotes Cells were selected and maintained in 10 µg/ml puromycin.
T365 6642-6656 Sentence denotes ELISAOne Assay
T366 6657-6789 Sentence denotes ELISAOne assays (TGRBio, Hindmarsh, Australia) were performed according to manufacturer’s protocol with the following modifications.
T367 6790-6863 Sentence denotes Cell lysates were prepared in RIPA buffer as described for Western blots.
T368 6864-6957 Sentence denotes Five microliters of samples were diluted in 45 µL of ELISAOne lysis buffer prior to analysis.
T369 6958-7075 Sentence denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473 were incubated with the samples for 2 hr at room temperature.
T370 7076-7135 Sentence denotes Primary antibody to pan-Akt was incubated overnight at 4°C.
T371 7136-7207 Sentence denotes Signals for phospho-antibodies were normalized based on pan-Akt values.
T372 7209-7219 Sentence denotes TNFα ELISA
T373 7220-7325 Sentence denotes Mouse TNFα Quantikine ELISA assays (R&D Systems) were performed according to manufacturer’s descriptions.
T374 7326-7406 Sentence denotes Cell lysates were prepared from 3×106 cells plated and treated in a 10 cm2 dish.
T375 7408-7433 Sentence denotes In vitro Akt Kinase Assay
T376 7434-7546 Sentence denotes Akt kinase activity was measured using the Akt kinase assay kit (nonradioactive) from Cell Signaling Technology.
T377 7547-7646 Sentence denotes In brief, Myr-Akt was immunoprecipitated from L929 cells using anti-FLAG M2 magnetic beads (Sigma).
T378 7647-7730 Sentence denotes The in vitro assay was performed in the presence of a GSK fusion protein substrate.
T379 7731-7804 Sentence denotes Phosphorylation of the GSK fusion protein was visualized by western blot.

simple1

Id Subject Object Predicate Lexical cue
T28182 7226-7230 Protein denotes TNFα
T28181 7209-7213 Protein denotes TNFα
T28295 7557-7564 Protein denotes Myr-Akt
T27783 6226-6234 Protein denotes Myr-Akt1
T27100 4936-4940 Protein denotes TNFα
T27099 4777-4781 Protein denotes TNFα
T26747 4532-4536 Protein denotes TNFα
T26746 4333-4338 Protein denotes Mapk9
T26745 4306-4311 Protein denotes Mapk8
T26744 4243-4247 Protein denotes PDK1
T26743 4191-4195 Protein denotes Akt3
T26742 4165-4169 Protein denotes Akt2
T26741 4139-4143 Protein denotes Akt1
T26014 2671-2674 Protein denotes 18S
T26013 2623-2626 Protein denotes 18S
T26012 2573-2577 Protein denotes TNFα
T26011 2522-2526 Protein denotes TNFα
T26010 2432-2435 Protein denotes 18S
T26009 2347-2351 Protein denotes TNFα
T26207 2733-2737 Protein denotes FADD
T25151 1012-1017 Protein denotes IGF-1
T25150 972-975 Protein denotes EGF
T25149 955-959 Protein denotes bFGF
T25148 932-936 Protein denotes TNFα
T25601 1331-1339 Protein denotes Myr-Akt1
T25600 1186-1199 Protein denotes Myr-Akt1-FLAG
T25599 1119-1127 Protein denotes Myr-Akt1

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T28363 7569-7587 Binding denotes immunoprecipitated
T28362 7557-7564 Protein denotes Myr-Akt
T28218 7226-7230 Protein denotes TNFα
T28217 7209-7213 Protein denotes TNFα
T27858 6226-6234 Protein denotes Myr-Akt1
T27318 4936-4940 Protein denotes TNFα
T27317 4777-4781 Protein denotes TNFα
T26067 2671-2674 Protein denotes 18S
T26066 2623-2626 Protein denotes 18S
T26065 2573-2577 Protein denotes TNFα
T26064 2522-2526 Protein denotes TNFα
T26063 2432-2435 Protein denotes 18S
T26062 2347-2351 Protein denotes TNFα
T26872 4532-4536 Protein denotes TNFα
T26871 4333-4338 Protein denotes Mapk9
T26870 4306-4311 Protein denotes Mapk8
T26869 4243-4247 Protein denotes PDK1
T26868 4191-4195 Protein denotes Akt3
T26867 4165-4169 Protein denotes Akt2
T26866 4139-4143 Protein denotes Akt1
T26327 2738-2747 Negative_regulation denotes deficient
T26326 2733-2737 Protein denotes FADD
T25253 1012-1017 Protein denotes IGF-1
T25252 972-975 Protein denotes EGF
T25251 955-959 Protein denotes bFGF
T25250 932-936 Protein denotes TNFα
T25650 1331-1339 Protein denotes Myr-Akt1
T25649 1186-1199 Protein denotes Myr-Akt1-FLAG
T25648 1119-1127 Protein denotes Myr-Akt1
R18019 T26326 T26327 themeOf FADD,deficient
R19678 T28362 T28363 themeOf Myr-Akt,immunoprecipitated

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T26086 2573-2577 Protein denotes TNFα
T26085 2522-2526 Protein denotes TNFα
T26084 2432-2435 Protein denotes 18S
T26083 2347-2351 Protein denotes TNFα
T25659 1331-1339 Protein denotes Myr-Akt1
T25658 1186-1199 Protein denotes Myr-Akt1-FLAG
T25531 1012-1017 Protein denotes IGF-1
T25530 972-975 Protein denotes EGF
T25529 955-959 Protein denotes bFGF
T25528 932-936 Protein denotes TNFα
T25657 1119-1127 Protein denotes Myr-Akt1
T26333 2738-2747 Negative_regulation denotes deficient
T26332 2733-2737 Protein denotes FADD
T26088 2671-2674 Protein denotes 18S
T26087 2623-2626 Protein denotes 18S
T26893 4306-4311 Protein denotes Mapk8
T26892 4243-4247 Protein denotes PDK1
T26891 4191-4195 Protein denotes Akt3
T26890 4165-4169 Protein denotes Akt2
T26889 4139-4143 Protein denotes Akt1
T28380 7569-7587 Binding denotes immunoprecipitated
T28379 7557-7564 Protein denotes Myr-Akt
T28226 7226-7230 Protein denotes TNFα
T28225 7209-7213 Protein denotes TNFα
T27861 6226-6234 Protein denotes Myr-Akt1
T27326 4936-4940 Protein denotes TNFα
T27325 4777-4781 Protein denotes TNFα
T26895 4532-4536 Protein denotes TNFα
T26894 4333-4338 Protein denotes Mapk9
R18022 T26332 T26333 themeOf FADD,deficient
R19680 T28379 T28380 themeOf Myr-Akt,immunoprecipitated

DLUT931

Id Subject Object Predicate Lexical cue
T28377 7569-7587 Binding denotes immunoprecipitated
T28376 7557-7564 Protein denotes Myr-Akt
T28224 7226-7230 Protein denotes TNFα
T28223 7209-7213 Protein denotes TNFα
T27859 6226-6234 Protein denotes Myr-Akt1
T27322 4936-4940 Protein denotes TNFα
T27321 4777-4781 Protein denotes TNFα
T26076 2671-2674 Protein denotes 18S
T26075 2623-2626 Protein denotes 18S
T26074 2573-2577 Protein denotes TNFα
T26073 2522-2526 Protein denotes TNFα
T26072 2432-2435 Protein denotes 18S
T26071 2347-2351 Protein denotes TNFα
T26881 4532-4536 Protein denotes TNFα
T26880 4333-4338 Protein denotes Mapk9
T26879 4306-4311 Protein denotes Mapk8
T26878 4243-4247 Protein denotes PDK1
T26877 4191-4195 Protein denotes Akt3
T26876 4165-4169 Protein denotes Akt2
T26875 4139-4143 Protein denotes Akt1
T26329 2738-2747 Negative_regulation denotes deficient
T26328 2733-2737 Protein denotes FADD
T25262 1012-1017 Protein denotes IGF-1
T25261 972-975 Protein denotes EGF
T25260 955-959 Protein denotes bFGF
T25259 932-936 Protein denotes TNFα
T25653 1331-1339 Protein denotes Myr-Akt1
T25652 1186-1199 Protein denotes Myr-Akt1-FLAG
T25651 1119-1127 Protein denotes Myr-Akt1
R18020 T26328 T26329 themeOf FADD,deficient
R19679 T28376 T28377 themeOf Myr-Akt,immunoprecipitated

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T28409 7712-7729 Protein denotes protein substrate
T28408 7477-7480 Protein denotes Akt
T28407 7417-7420 Protein denotes Akt
T28406 7557-7564 Protein denotes Myr-Akt
T28405 7620-7622 Entity denotes M2
T28404 7747-7772 Protein denotes of the GSK fusion protein
T28403 7408-7433 Protein denotes In vitro Akt Kinase Assay
T28402 7434-7437 Protein denotes Akt
T28401 7520-7545 Entity denotes Cell Signaling Technology
T28400 7481-7487 Protein denotes kinase
T28399 7639-7644 Protein denotes Sigma
T28398 7434-7444 Protein denotes Akt kinase
T28397 7712-7729 Entity denotes protein substrate
T28069 6790-6862 Entity denotes Cell lysates were prepared in RIPA buffer as described for Western blots
T28068 7136-7143 Entity denotes Signals
T28067 6999-7013 Entity denotes phopsho-Ser473
T28066 7192-7206 Protein denotes pan-Akt values
T28065 7096-7103 Protein denotes pan-Akt
T28064 7076-7092 Entity denotes Primary antibody
T28063 7148-7166 Entity denotes phospho-antibodies
T28062 6674-6677 Protein denotes TGR
T28061 6657-6703 Entity denotes ELISAOne assays (TGRBio, Hindmarsh, Australia)
T28060 6980-6994 Entity denotes phopsho-Thr308
T28059 6958-7013 Entity denotes Primary antibodies to phopsho-Thr308 and phopsho-Ser473
T27877 6206-6234 Regulation denotes Stable Infection of Myr-Akt1
T27876 6316-6333 Entity denotes 2 µg of viral DNA
T27875 6621-6639 Entity denotes 10 µg/ml puromycin
T27874 6266-6293 Entity denotes HEK293FT cells (Invitrogen)
T27873 6583-6588 Entity denotes Cells
T27872 6406-6449 Entity denotes GenJet transfection reagent (Signagen Labs)
T27871 6223-6234 Protein denotes of Myr-Akt1
T27870 6321-6333 Entity denotes of viral DNA
T27869 6564-6581 Entity denotes 8 µg/ml polybrene
T27868 6406-6409 Protein denotes Gen
T28271 7392-7405 Protein denotes a 10 cm2 dish
T28270 7326-7338 Entity denotes Cell lysates
T28269 7209-7213 Protein denotes TNFα
T28268 7220-7241 Protein denotes Mouse TNFα Quantikine
T28267 7358-7405 Entity denotes 3×106 cells plated and treated in a 10 cm2 dish
T27345 4649-4667 Entity denotes 1×106 Jurkat cells
T27344 5133-5155 Entity denotes Protein concentrations
T27343 5496-5514 Entity denotes Primary antibodies
T27342 4744-4758 Entity denotes 30 µM zVAD.fmk
T27341 5382-5386 Entity denotes PVDF
T27340 5419-5448 Protein denotes 5% bovine serum albumin (BSA)
T27339 5232-5243 Protein denotes of proteins
T27693 5830-5837 Protein denotes qRT-PCR
T27692 5889-5898 Protein denotes Total RNA
T27691 6091-6105 Protein denotes qPCR reactions
T27690 5959-5962 Protein denotes RNA
T27689 5838-5843 Entity denotes Cells
T27366 4942-4972 Binding denotes Cells were harvested in 1×RIPA
T27365 4609-4699 Binding denotes For Western blot, 4×105 adherent cells (1×106 Jurkat cells) were seeded into 35 mm2 dishes
T27364 5191-5216 Entity denotes nm Assay Reagent (Pierce)
T27363 4827-4832 Entity denotes cells
T27362 5659-5671 Entity denotes ECL reagents
T27361 5382-5395 Entity denotes PVDF membrane
T27360 5562-5582 Entity denotes Secondary antibodies
T27359 4627-4668 Entity denotes 4×105 adherent cells (1×106 Jurkat cells)
T27358 4717-4722 Entity denotes cells
T27357 5779-5781 Entity denotes GM
T27356 4942-4947 Entity denotes Cells
T27355 5813-5827 Entity denotes new antibodies
T27354 4981-4985 Entity denotes Cell
T27353 4909-4917 Entity denotes zVAD.fmk
T27352 5079-5091 Entity denotes cell lysates
T27351 5659-5662 Protein denotes ECL
T27350 5721-5730 Entity denotes membranes
T27349 4762-4781 Protein denotes 10 ng/ml mouse TNFα
T27348 4903-4940 Protein denotes 20 µM zVAD.fmk or 10 ng/ml mouse TNFα
T27347 5348-5361 Entity denotes SDS-PAGE gels
T27346 5444-5447 Protein denotes BSA
T26928 4133-4157 Protein denotes mouse Akt1 (L-040709-00)
T26927 4091-4093 Entity denotes S6
T26926 4496-4501 Entity denotes cells
T26925 4408-4437 Entity denotes RNAiMAX reagent (Invitrogen),
T26924 4075-4131 Protein denotes Mouse ribosomal S6 protein (L-040893-00 and L-045791-00)
T26923 4532-4567 Protein denotes TNFα for 9 hr (RNA or Western blot)
T26922 4408-4437 Protein denotes RNAiMAX reagent (Invitrogen),
T26921 4578-4592 Entity denotes cell viability
T26920 4520-4528 Entity denotes zVAD.fmk
T26691 3999-4018 Entity denotes the small molecules
T26690 3579-3593 Entity denotes Cell viability
T26689 3615-3659 Entity denotes CellTiter-Glo Cell Viability Assay (Promega)
T26688 3905-3910 Entity denotes cells
T26687 3793-3801 Entity denotes of cells
T26686 3726-3756 Entity denotes of the control untreated cells
T26685 3405-3431 Entity denotes Cell Viability Experiments
T26684 3974-4018 Entity denotes non-specific toxicity of the small molecules
T26175 2567-2577 Protein denotes human TNFα
T26174 2400-2435 Protein denotes 5′-GCTACGACGTGGGCTACAG-3′;mouse 18S
T26173 2341-2351 Protein denotes Mouse TNFα
T26172 2636-2674 Protein denotes 5′- CAGCCACCCGAGATTGAGCA -3, human 18S
T26171 2617-2626 Protein denotes human 18S
T26170 2516-2526 Protein denotes human TNFα
T25746 1381-1391 Entity denotes Antibodies
T26473 2786-2802 Entity denotes Lung fibroblasts
T26472 3242-3267 Entity denotes non-essential amino acids
T26471 3273-3288 Entity denotes sodium pyruvate
T26470 3290-3302 Entity denotes Jurkat cells
T26469 2872-2892 Entity denotes J774A.1 (ATCC) cells
T26468 2733-2760 Entity denotes FADD-deficient Jurkat cells
T26467 2897-2918 Entity denotes RAW264.7 (ATCC) cells
T26466 2724-2728 Entity denotes L929
T26465 3177-3187 Entity denotes fibroblast
T26464 3377-3402 Entity denotes 1% antibiotic-antimycotic
T26463 3229-3240 Entity denotes L-glutamine
T26462 3105-3108 Protein denotes FBS
T26461 3033-3038 Entity denotes Cells
T26460 3114-3160 Entity denotes 1% antibiotic-antimycotic mixture (Invitrogen)
T25670 1254-1259 Entity denotes EcoRI
T25669 1244-1249 Entity denotes BglII
T25668 1186-1199 Protein denotes Myr-Akt1-FLAG
T25667 1214-1220 Protein denotes by PCR
T25666 1123-1127 Protein denotes Akt1
T25665 1104-1107 Entity denotes DNA
T25664 1328-1339 Protein denotes of Myr-Akt1
T25663 1151-1184 Protein denotes FLAG tag, has been described [52]
T25662 1119-1122 Entity denotes Myr
T25661 1116-1150 Entity denotes of Myr-Akt1, containing c-terminal
T25660 1116-1150 Entity denotes of Myr-Akt1, containing c-terminal
T25569 847-914 Binding denotes Pan-caspase inhibitor zVAD.fmk (20–30 µM) was purchased from Bachem
T25568 859-868 Entity denotes inhibitor
T25567 926-936 Protein denotes mouse TNFα
T25566 972-975 Protein denotes EGF
T25565 119-141 Entity denotes The following reagents
T25564 949-959 Protein denotes human bFGF
T25563 847-888 Protein denotes Pan-caspase inhibitor zVAD.fmk (20–30 µM)
T25562 847-888 Entity denotes Pan-caspase inhibitor zVAD.fmk (20–30 µM)
T25561 988-995 Entity denotes PDGF-BB
T25560 23-31 Entity denotes Reagents
T25559 1067-1085 Entity denotes All other reagents
T25558 1039-1043 Entity denotes Cell
T25557 1012-1017 Protein denotes IGF-1
T25556 1096-1101 Protein denotes Sigma
R17464 T25562 T25569 themeOf Pan-caspase inhibitor zVAD.fmk (20–30 µM),Pan-caspase inhibitor zVAD.fmk (20–30 µM) was purchased from Bachem
R19285 T27871 T27877 themeOf of Myr-Akt1,Stable Infection of Myr-Akt1

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T26683 4018-4019 . denotes .
T27575 5638-5646 NNP denotes Luminata
T28423 7481-7487 NN denotes kinase
T28479 7803-7804 . denotes .
T28478 7799-7803 NN denotes blot
T28477 7791-7798 JJ denotes western
T28476 7788-7790 IN denotes by
T28475 7777-7787 VBN denotes visualized
T28474 7773-7776 VBD denotes was
T28473 7765-7772 NN denotes protein
T28472 7758-7764 NN denotes fusion
T28471 7754-7757 NNP denotes GSK
T28470 7750-7753 DT denotes the
T28469 7747-7749 IN denotes of
T28468 7731-7746 NNP denotes Phosphorylation
T28467 7729-7730 . denotes .
T28466 7720-7729 NN denotes substrate
T28465 7712-7719 NN denotes protein
T28464 7705-7711 NN denotes fusion
T28463 7701-7704 NNP denotes GSK
T28462 7699-7700 DT denotes a
T28461 7696-7698 IN denotes of
T28460 7687-7695 NN denotes presence
T28459 7683-7686 DT denotes the
T28458 7680-7682 IN denotes in
T28457 7670-7679 VBN denotes performed
T28456 7666-7669 VBD denotes was
T28455 7660-7665 NN denotes assay
T28454 7654-7659 NN denotes vitro
T28453 7651-7653 IN denotes in
T28452 7647-7650 DT denotes The
T28451 7645-7646 . denotes .
T28450 7644-7645 -RRB- denotes )
T28449 7639-7644 NNP denotes Sigma
T28448 7638-7639 -LRB- denotes (
T28447 7632-7637 NNS denotes beads
T28446 7623-7631 JJ denotes magnetic
T28445 7620-7622 NN denotes M2
T28444 7610-7619 JJ denotes anti-FLAG
T28443 7604-7609 VBG denotes using
T28442 7598-7603 NNS denotes cells
T28441 7593-7597 CD denotes L929
T28440 7588-7592 IN denotes from
T28439 7569-7587 VBN denotes immunoprecipitated
T28438 7565-7568 VBD denotes was
T28437 7557-7564 NNP denotes Myr-Akt
T28436 7555-7556 , denotes ,
T28435 7550-7555 NN denotes brief
T28434 7547-7549 IN denotes In
T28433 7545-7546 . denotes .
T28432 7535-7545 NNP denotes Technology
T28431 7525-7534 NNP denotes Signaling
T28430 7520-7524 NNP denotes Cell
T28429 7515-7519 IN denotes from
T28428 7513-7514 -RRB- denotes )
T28427 7499-7513 JJ denotes nonradioactive
T28426 7498-7499 -LRB- denotes (
T28425 7494-7497 NN denotes kit
T28424 7488-7493 NN denotes assay
T28422 7477-7480 NNP denotes Akt
T28421 7473-7476 DT denotes the
T28420 7467-7472 VBG denotes using
T28419 7458-7466 VBN denotes measured
T28418 7454-7457 VBD denotes was
T28417 7445-7453 NN denotes activity
T28416 7438-7444 NN denotes kinase
T28415 7434-7437 NNP denotes Akt
T28414 7428-7433 NNP denotes Assay
T28413 7421-7427 NNP denotes Kinase
T28412 7417-7420 NNP denotes Akt
T28411 7411-7416 NN denotes vitro
T28410 7408-7410 IN denotes In
T28244 7297-7309 NN denotes manufacturer
T28243 7294-7296 TO denotes to
T28242 7284-7293 VBG denotes according
T28241 7274-7283 VBN denotes performed
T28240 7269-7273 VBD denotes were
T28239 7267-7268 -RRB- denotes )
T28238 7260-7267 NNPS denotes Systems
T28237 7256-7259 NNP denotes R&D
T28236 7255-7256 -LRB- denotes (
T28235 7248-7254 NNS denotes assays
T28234 7242-7247 NNP denotes ELISA
T28233 7231-7241 NNP denotes Quantikine
T28232 7226-7230 NNP denotes TNFα
T28231 7220-7225 NNP denotes Mouse
T28230 7214-7219 NNP denotes ELISA
T28229 7209-7213 NNP denotes TNFα
T28160 7206-7207 . denotes .
T28159 7200-7206 NNS denotes values
T28158 7192-7199 JJ denotes pan-Akt
T28157 7189-7191 IN denotes on
T28156 7183-7188 VBN denotes based
T28155 7172-7182 VBN denotes normalized
T28154 7167-7171 VBD denotes were
T28153 7148-7166 NNS denotes phospho-antibodies
T28152 7144-7147 IN denotes for
T28151 7136-7143 NNP denotes Signals
T28150 7133-7135 NNP denotes C.
T28149 7132-7133 CD denotes °
T28148 7131-7132 CD denotes 4
T28147 7128-7130 IN denotes at
T28146 7118-7127 RB denotes overnight
T28145 7108-7117 VBN denotes incubated
T28144 7104-7107 VBD denotes was
T28143 7096-7103 NN denotes pan-Akt
T28142 7093-7095 TO denotes to
T28141 7084-7092 NN denotes antibody
T28140 7076-7083 JJ denotes Primary
T28139 7074-7075 . denotes .
T28138 7063-7074 NN denotes temperature
T28137 7058-7062 NN denotes room
T28136 7055-7057 IN denotes at
T28135 7052-7054 NN denotes hr
T28134 7050-7051 CD denotes 2
T28133 7046-7049 IN denotes for
T28132 7038-7045 NNS denotes samples
T28131 7034-7037 DT denotes the
T28130 7029-7033 IN denotes with
T28129 7019-7028 VBN denotes incubated
T28128 7014-7018 VBD denotes were
T28127 6999-7013 JJ denotes phopsho-Ser473
T28126 6995-6998 CC denotes and
T28125 6980-6994 JJ denotes phopsho-Thr308
T28124 6977-6979 TO denotes to
T28123 6966-6976 NNS denotes antibodies
T28122 6958-6965 JJ denotes Primary
T28121 6956-6957 . denotes .
T28120 6948-6956 NN denotes analysis
T28119 6945-6947 TO denotes to
T28118 6939-6944 RB denotes prior
T28117 6932-6938 NN denotes buffer
T28116 6926-6931 VBZ denotes lysis
T28107 6881-6883 IN denotes of
T28106 6869-6880 NNS denotes microliters
T28105 6864-6868 CD denotes Five
T28104 6862-6863 . denotes .
T28103 6857-6862 NNS denotes blots
T28102 6849-6856 JJ denotes Western
T28101 6845-6848 IN denotes for
T28100 6835-6844 VBN denotes described
T28099 6832-6834 IN denotes as
T28098 6825-6831 NN denotes buffer
T28097 6820-6824 NNP denotes RIPA
T28096 6817-6819 IN denotes in
T28095 6808-6816 VBN denotes prepared
T28094 6803-6807 VBD denotes were
T28093 6795-6802 NNS denotes lysates
T28092 6790-6794 NN denotes Cell
T28091 6788-6789 . denotes .
T28090 6775-6788 NNS denotes modifications
T28089 6765-6774 VBG denotes following
T28088 6761-6764 DT denotes the
T28087 6756-6760 IN denotes with
T28086 6747-6755 NN denotes protocol
T28085 6732-6744 NN denotes manufacturer
T28084 6729-6731 TO denotes to
T28083 6719-6728 VBG denotes according
T28082 6709-6718 VBN denotes performed
T28081 6704-6708 VBD denotes were
T28080 6702-6703 -RRB- denotes )
T28079 6693-6702 NNP denotes Australia
T28078 6691-6692 , denotes ,
T28077 6682-6691 NNP denotes Hindmarsh
T28076 6680-6681 , denotes ,
T28075 6674-6680 NNP denotes TGRBio
T28074 6673-6674 -LRB- denotes (
T28073 6666-6672 NNS denotes assays
T28072 6657-6665 NNP denotes ELISAOne
T28071 6651-6656 NNP denotes Assay
T28070 6642-6650 NNP denotes ELISAOne
T27958 6639-6640 . denotes .
T27957 6630-6639 NN denotes puromycin
T27956 6627-6629 NN denotes ml
T27955 6626-6627 NN denotes /
T27954 6624-6626 NN denotes µg
T27953 6621-6623 CD denotes 10
T27952 6618-6620 IN denotes in
T27951 6607-6617 VBN denotes maintained
T27950 6603-6606 CC denotes and
T27949 6594-6602 VBN denotes selected
T27948 6589-6593 VBD denotes were
T27947 6583-6588 NNS denotes Cells
T27946 6581-6582 . denotes .
T27945 6572-6581 NN denotes polybrene
T27944 6569-6571 NN denotes ml
T27943 6568-6569 NN denotes /
T27942 6566-6568 NN denotes µg
T27941 6564-6565 CD denotes 8
T27940 6559-6563 IN denotes with
T27939 6553-6558 NNS denotes cells
T27938 6548-6552 CD denotes L929
T27937 6545-6547 TO denotes to
T27936 6537-6544 VBD denotes applied
T27935 6533-6536 CC denotes and
T27934 6526-6532 NN denotes filter
T27933 6523-6525 NN denotes µm
T27932 6518-6522 CD denotes 0.45
T27931 6510-6517 IN denotes through
T27930 6501-6509 VBN denotes filtered
T27929 6499-6500 , denotes ,
T27928 6494-6499 RB denotes later
T27927 6491-6493 NN denotes hr
T27926 6488-6490 CD denotes 72
T27925 6478-6487 VBN denotes collected
T27924 6474-6477 VBD denotes was
T27923 6468-6473 NNS denotes media
T27922 6451-6467 VBG denotes Virus-containing
T27921 6449-6450 . denotes .
T27920 6448-6449 -RRB- denotes )
T27919 6444-6448 NNPS denotes Labs
T27918 6435-6443 NNP denotes Signagen
T27917 6434-6435 -LRB- denotes (
T27916 6426-6433 NN denotes reagent
T27915 6413-6425 NN denotes transfection
T27914 6406-6412 NNP denotes GenJet
T27913 6400-6405 VBG denotes using
T27912 6393-6399 VBZ denotes plates
T27911 6388-6392 RB denotes well
T27910 6386-6387 CD denotes 6
T27909 6383-6385 IN denotes in
T27908 6374-6382 NNS denotes plasmids
T27907 6364-6373 JJ denotes accessory
T27906 6358-6363 NNP denotes VSV-G
T27905 6354-6357 CC denotes and
T27904 6346-6353 NN denotes gal/pol
T27903 6343-6345 IN denotes of
T27902 6340-6342 NN denotes µg
T27901 6338-6339 CD denotes 1
T27900 6334-6337 CC denotes and
T27899 6330-6333 NN denotes DNA
T27898 6324-6329 JJ denotes viral
T27897 6321-6323 IN denotes of
T27896 6318-6320 NN denotes µg
T27895 6316-6317 CD denotes 2
T27894 6311-6315 IN denotes with
T27893 6299-6310 VBN denotes transfected
T27892 6294-6298 VBD denotes were
T27891 6292-6293 -RRB- denotes )
T27890 6282-6292 NNP denotes Invitrogen
T27889 6281-6282 -LRB- denotes (
T27888 6275-6280 NNS denotes cells
T27887 6266-6274 JJ denotes HEK293FT
T27886 6264-6265 , denotes ,
T27885 6252-6264 NNS denotes retroviruses
T27884 6247-6251 NNP denotes MSCV
T27883 6238-6246 VB denotes generate
T27882 6235-6237 TO denotes To
T27881 6226-6234 NNP denotes Myr-Akt1
T27880 6223-6225 IN denotes of
T27879 6213-6222 NN denotes Infection
T27878 6206-6212 JJ denotes Stable
T28264 7405-7406 . denotes .
T28263 7401-7405 NN denotes dish
T28262 7397-7400 NN denotes cm2
T28261 7394-7396 CD denotes 10
T28260 7392-7393 DT denotes a
T28259 7389-7391 IN denotes in
T28258 7381-7388 VBN denotes treated
T28257 7377-7380 CC denotes and
T28256 7370-7376 VBN denotes plated
T28255 7364-7369 NNS denotes cells
T28254 7360-7363 CD denotes 106
T28253 7359-7360 CD denotes ×
T28252 7358-7359 CD denotes 3
T28251 7353-7357 IN denotes from
T28250 7344-7352 VBN denotes prepared
T28249 7339-7343 VBD denotes were
T28248 7331-7338 NNS denotes lysates
T28247 7326-7330 NN denotes Cell
T28246 7324-7325 . denotes .
T28245 7312-7324 NNS denotes descriptions
T27608 5827-5828 . denotes .
T27607 5817-5827 NNS denotes antibodies
T27606 5813-5816 JJ denotes new
T27605 5808-5812 IN denotes with
T27604 5799-5807 VBN denotes reprobed
T27603 5795-5798 CC denotes and
T27602 5793-5794 -RRB- denotes )
T27601 5782-5793 NNPS denotes Biosciences
T27600 5779-5781 NNP denotes GM
T27599 5778-5779 -LRB- denotes (
T27598 5771-5777 NN denotes buffer
T27597 5761-5770 VBG denotes stripping
T27596 5751-5760 NNP denotes OneMinute
T27595 5745-5750 VBG denotes using
T27594 5736-5744 VBN denotes stripped
T27593 5731-5735 VBD denotes were
T27592 5721-5730 NNS denotes membranes
T27591 5719-5720 , denotes ,
T27590 5714-5719 NNS denotes cases
T27589 5709-5713 DT denotes some
T27588 5706-5708 IN denotes In
T27587 5704-5705 . denotes .
T27586 5697-5704 NNS denotes signals
T27585 5693-5696 DT denotes the
T27584 5685-5692 VB denotes develop
T27583 5682-5684 TO denotes to
T27582 5677-5681 VBN denotes used
T27581 5672-5676 VBD denotes were
T27580 5663-5671 NNS denotes reagents
T27579 5659-5662 NNP denotes ECL
T27578 5657-5658 -RRB- denotes )
T27577 5648-5657 NNP denotes Millipore
T27576 5647-5648 -LRB- denotes (
T27574 5636-5637 . denotes .
T27573 5625-5636 NN denotes temperature
T27572 5620-5624 NN denotes room
T27571 5617-5619 IN denotes at
T27570 5613-5616 NN denotes min
T27569 5610-5612 CD denotes 30
T27568 5606-5609 IN denotes for
T27567 5601-5605 NNP denotes TBST
T27566 5598-5600 IN denotes in
T27565 5588-5597 VBN denotes incubated
T27564 5583-5587 VBD denotes were
T27563 5572-5582 NNS denotes antibodies
T27562 5562-5571 JJ denotes Secondary
T27561 5559-5561 NNP denotes C.
T27560 5558-5559 CD denotes °
T27559 5557-5558 CD denotes 4
T27558 5554-5556 IN denotes at
T27557 5544-5553 JJ denotes overnight
T27556 5535-5543 NNP denotes BSA/TBST
T27555 5534-5535 NN denotes %
T27554 5533-5534 CD denotes 5
T27553 5530-5532 IN denotes in
T27552 5520-5529 VBN denotes incubated
T27551 5515-5519 VBD denotes were
T27550 5504-5514 NNS denotes antibodies
T27549 5496-5503 JJ denotes Primary
T27548 5494-5495 . denotes .
T27547 5483-5494 NN denotes temperature
T27546 5478-5482 NN denotes room
T27545 5475-5477 IN denotes at
T27544 5471-5474 NN denotes min
T27543 5468-5470 CD denotes 30
T27542 5464-5467 IN denotes for
T27541 5457-5463 NN denotes buffer
T27540 5452-5456 NNP denotes TBST
T27539 5449-5451 IN denotes in
T27538 5447-5448 -RRB- denotes )
T27537 5444-5447 NNP denotes BSA
T27536 5443-5444 -LRB- denotes (
T27535 5435-5442 NN denotes albumin
T27534 5429-5434 NN denotes serum
T27533 5422-5428 JJ denotes bovine
T27532 5420-5421 NN denotes %
T27531 5419-5420 CD denotes 5
T27530 5416-5418 CC denotes or
T27529 5411-5415 NN denotes milk
T27528 5409-5410 NN denotes %
T27527 5408-5409 CD denotes 3
T27526 5405-5407 IN denotes in
T27525 5397-5404 VBN denotes blocked
T27524 5395-5396 , denotes ,
T27523 5387-5395 NN denotes membrane
T27522 5382-5386 NNP denotes PVDF
T27521 5379-5381 TO denotes to
T27520 5367-5378 VBN denotes transferred
T27519 5362-5366 VBD denotes were
T27518 5357-5361 NNS denotes gels
T27517 5348-5356 NNP denotes SDS-PAGE
T27516 5346-5347 , denotes ,
T27515 5339-5346 RB denotes Briefly
T27514 5337-5338 . denotes .
T27513 5328-5337 NNS denotes protocols
T27512 5319-5327 JJ denotes standard
T27511 5316-5318 TO denotes to
T27510 5306-5315 VBG denotes according
T27509 5296-5305 VBN denotes performed
T27508 5292-5295 VBD denotes was
T27507 5283-5291 VBG denotes blotting
T27506 5275-5282 NNP denotes Western
T27505 5272-5274 NNP denotes C.
T27504 5271-5272 CD denotes °
T27503 5269-5271 CD denotes 95
T27502 5266-5268 IN denotes at
T27501 5262-5265 NN denotes min
T27500 5260-5261 CD denotes 5
T27499 5256-5259 IN denotes for
T27498 5249-5255 VBN denotes boiled
T27497 5244-5248 VBD denotes were
T27496 5235-5243 NNS denotes proteins
T27495 5232-5234 IN denotes of
T27494 5224-5231 NNS denotes amounts
T27493 5218-5223 NNP denotes Equal
T27492 5216-5217 . denotes .
T27491 5215-5216 -RRB- denotes )
T27490 5209-5215 NNP denotes Pierce
T27489 5208-5209 -LRB- denotes (
T27488 5200-5207 NNP denotes Reagent
T27487 5194-5199 NNP denotes Assay
T27486 5191-5193 NN denotes nm
T27485 5187-5190 CD denotes 660
T27484 5180-5186 NNP denotes Pierce
T27483 5176-5179 DT denotes the
T27482 5170-5175 VBG denotes using
T27481 5161-5169 VBN denotes measured
T27480 5156-5160 VBD denotes were
T27479 5141-5155 NNS denotes concentrations
T27478 5133-5140 NN denotes Protein
T27477 5131-5132 . denotes .
T27476 5128-5131 NN denotes rpm
T27475 5127-5128 CD denotes ×
T27474 5121-5127 CD denotes 14,000
T27473 5118-5120 IN denotes at
T27472 5114-5117 NN denotes min
T27471 5111-5113 CD denotes 15
T27470 5107-5110 IN denotes for
T27469 5102-5106 RP denotes down
T27468 5097-5101 VBN denotes spun
T27467 5092-5096 VBD denotes were
T27466 5084-5091 NNS denotes lysates
T27465 5079-5083 NN denotes cell
T27464 5077-5078 , denotes ,
T27463 5067-5077 NN denotes sonication
T27462 5061-5066 JJ denotes brief
T27461 5055-5060 IN denotes After
T27460 5053-5054 . denotes .
T27770 6203-6204 . denotes .
T27769 6202-6203 -RRB- denotes )
T27768 6197-6202 NNP denotes Roche
T27767 6196-6197 -LRB- denotes (
T27766 6181-6195 NNP denotes LightCycler480
T27765 6179-6180 DT denotes a
T27764 6176-6178 IN denotes in
T27763 6174-6175 -RRB- denotes )
T27762 6161-6174 NNS denotes SABiosciences
T27761 6160-6161 -LRB- denotes (
T27760 6156-6159 NN denotes mix
T27759 6149-6155 NNP denotes Master
T27758 6148-6149 CD denotes ×
T27757 6147-6148 CD denotes 2
T27756 6137-6146 NNP denotes SYBRGreen
T27755 6131-6136 VBG denotes using
T27754 6122-6130 VBZ denotes performs
T27753 6117-6121 VBD denotes were
T27752 6107-6116 NNS denotes Reactions
T27751 6105-6106 . denotes .
T27750 6096-6105 NNS denotes reactions
T27749 6091-6095 NNP denotes qPCR
T27748 6088-6090 IN denotes in
T27747 6080-6087 NNS denotes primers
T27746 6077-6079 NN denotes pM
T27745 6073-6076 CD denotes 500
T27744 6068-6072 IN denotes with
T27743 6063-6067 VBN denotes used
T27742 6059-6062 VBD denotes was
T27741 6054-6058 NNP denotes cDNA
T27740 6051-6053 IN denotes of
T27739 6048-6050 NN denotes µL
T27738 6046-6047 CD denotes 1
T27737 6044-6045 . denotes .
T27736 6043-6044 -RRB- denotes )
T27735 6036-6043 NNPS denotes Biolabs
T27734 6028-6035 NNP denotes England
T27733 6024-6027 NNP denotes New
T27732 6022-6023 , denotes ,
T27731 6019-6022 NN denotes kit
T27730 6014-6018 NNP denotes cDNA
T27729 6007-6013 NNP denotes M-MuLV
T27728 6006-6007 -LRB- denotes (
T27727 5998-6005 NNS denotes primers
T27726 5991-5997 JJ denotes random
T27725 5985-5990 VBG denotes using
T27724 5980-5984 NNP denotes cDNA
T27723 5977-5979 TO denotes to
T27722 5967-5976 VBN denotes converted
T27721 5963-5966 VBD denotes was
T27720 5959-5962 NNP denotes RNA
T27719 5956-5958 IN denotes of
T27718 5953-5955 NN denotes µg
T27717 5951-5952 CD denotes 1
T27716 5949-5950 . denotes .
T27715 5948-5949 -RRB- denotes )
T27714 5940-5948 NNP denotes Research
T27713 5935-5939 NNP denotes Zymo
T27712 5934-5935 -LRB- denotes (
T27711 5930-5933 NN denotes kit
T27710 5921-5929 NNP denotes Miniprep
T27709 5918-5920 NNP denotes ZR
T27708 5912-5917 VBG denotes using
T27707 5903-5911 VBN denotes isolated
T27706 5899-5902 VBD denotes was
T27705 5895-5898 NNP denotes RNA
T27704 5889-5894 JJ denotes Total
T27703 5887-5888 . denotes .
T27702 5882-5887 NNS denotes blots
T27701 5874-5881 JJ denotes Western
T27700 5870-5873 IN denotes for
T27699 5860-5869 VBN denotes described
T27698 5857-5859 IN denotes as
T27697 5849-5856 VBN denotes treated
T27696 5844-5848 VBD denotes were
T27695 5838-5843 NNPS denotes Cells
T27694 5830-5837 NNP denotes qRT-PCR
T27043 4593-4594 . denotes .
T27042 4592-4593 -RRB- denotes )
T27041 4583-4592 NN denotes viability
T27040 4578-4582 NN denotes cell
T27039 4577-4578 -LRB- denotes (
T27038 4574-4576 NN denotes hr
T27037 4571-4573 CD denotes 24
T27036 4568-4570 CC denotes or
T27035 4566-4567 -RRB- denotes )
T27034 4562-4566 NN denotes blot
T27033 4554-4561 NNP denotes Western
T27032 4551-4553 CC denotes or
T27031 4547-4550 NNP denotes RNA
T27030 4546-4547 -LRB- denotes (
T27029 4543-4545 NN denotes hr
T27028 4541-4542 CD denotes 9
T27027 4537-4540 IN denotes for
T27026 4532-4536 NNP denotes TNFα
T27025 4529-4531 CC denotes or
T27024 4520-4528 NNP denotes zVAD.fmk
T27023 4515-4519 IN denotes with
T27022 4507-4514 VBN denotes treated
T27021 4502-4506 VBD denotes were
T27020 4496-4501 NNS denotes cells
T27019 4494-4495 , denotes ,
T27018 4492-4494 NN denotes hr
T27017 4489-4491 CD denotes 72
T27016 4483-4488 IN denotes After
T27015 4481-4482 . denotes .
T27014 4466-4481 NNS denotes recommendations
T27013 4451-4463 NN denotes manufacturer
T27012 4448-4450 TO denotes to
T27011 4438-4447 VBG denotes according
T27010 4436-4437 , denotes ,
T27009 4435-4436 -RRB- denotes )
T27008 4425-4435 NNP denotes Invitrogen
T27007 4424-4425 -LRB- denotes (
T27006 4416-4423 NN denotes reagent
T27005 4408-4415 NNP denotes RNAiMAX
T27004 4402-4407 VBG denotes using
T27003 4390-4401 VBN denotes transfected
T27002 4385-4389 VBD denotes were
T27001 4379-4384 NN denotes siRNA
T27000 4377-4378 . denotes .
T26999 4376-4377 -RRB- denotes )
T26998 4365-4376 NN denotes L-043776-00
T26997 4364-4365 -LRB- denotes (
T26996 4360-4363 NN denotes Jun
T26995 4354-4359 NN denotes mouse
T26994 4352-4353 , denotes ,
T26993 4351-4352 -RRB- denotes )
T26992 4340-4351 NNP denotes J-040134-05
T26991 4339-4340 -LRB- denotes (
T26990 4333-4338 NNP denotes Mapk9
T26989 4327-4332 NN denotes mouse
T26988 4325-4326 , denotes ,
T26987 4324-4325 -RRB- denotes )
T26986 4313-4324 NNP denotes J-040128-05
T26985 4312-4313 -LRB- denotes (
T26984 4306-4311 NNP denotes Mapk8
T26983 4300-4305 NN denotes mouse
T26982 4298-4299 , denotes ,
T26981 4297-4298 -RRB- denotes )
T26980 4283-4297 NNP denotes D-001810-10-05
T26979 4282-4283 -LRB- denotes (
T26978 4274-4281 NN denotes control
T26977 4263-4273 JJ denotes non-coding
T26976 4261-4262 , denotes ,
T26975 4260-4261 -RRB- denotes )
T26974 4249-4260 NNP denotes L-040658-00
T26973 4248-4249 -LRB- denotes (
T26972 4243-4247 NNP denotes PDK1
T26971 4237-4242 NN denotes mouse
T26970 4235-4236 , denotes ,
T26969 4234-4235 -RRB- denotes )
T26968 4223-4234 NNP denotes L-065427-00
T26967 4222-4223 -LRB- denotes (
T26966 4217-4221 NNP denotes mTOR
T26965 4211-4216 NN denotes mouse
T26964 4209-4210 , denotes ,
T26963 4208-4209 -RRB- denotes )
T26962 4197-4208 NNP denotes L-040891-00
T26961 4196-4197 -LRB- denotes (
T26960 4191-4195 NNP denotes Akt3
T26959 4185-4190 NN denotes mouse
T26958 4183-4184 , denotes ,
T26957 4182-4183 -RRB- denotes )
T26956 4171-4182 NNP denotes L-040782-00
T26955 4170-4171 -LRB- denotes (
T26954 4165-4169 NNP denotes Akt2
T26953 4159-4164 NN denotes mouse
T26952 4157-4158 , denotes ,
T26951 4156-4157 -RRB- denotes )
T26950 4145-4156 NNP denotes L-040709-00
T26949 4144-4145 -LRB- denotes (
T26948 4139-4143 NNP denotes Akt1
T26947 4133-4138 NN denotes mouse
T26946 4131-4132 , denotes ,
T26945 4130-4131 -RRB- denotes )
T26944 4119-4130 NN denotes L-045791-00
T26943 4115-4118 CC denotes and
T26942 4103-4114 NN denotes L-040893-00
T26941 4102-4103 -LRB- denotes (
T26940 4094-4101 NN denotes protein
T26939 4091-4093 NNP denotes S6
T26938 4081-4090 JJ denotes ribosomal
T26937 4075-4080 NNP denotes Mouse
T26936 4073-4074 . denotes .
T26935 4064-4073 NNP denotes Dharmacon
T26934 4059-4063 IN denotes from
T27459 5024-5053 NN denotes phenylmethanesulfonylfluoride
T27458 5021-5023 NN denotes ml
T27457 5020-5021 NN denotes /
T27456 5018-5020 NN denotes µg
T27455 5015-5017 CD denotes 50
T27454 5010-5014 IN denotes with
T27453 4997-5009 VBN denotes supplemented
T27452 4995-4996 -RRB- denotes )
T27451 4986-4995 NNP denotes Signaling
T27450 4981-4985 NNP denotes Cell
T27449 4980-4981 -LRB- denotes (
T27448 4973-4979 NN denotes buffer
T27447 4968-4972 NNP denotes RIPA
T27446 4967-4968 CD denotes ×
T27445 4966-4967 CD denotes 1
T27444 4963-4965 IN denotes in
T27443 4953-4962 VBN denotes harvested
T27442 4948-4952 VBD denotes were
T27441 4942-4947 NNS denotes Cells
T27440 4940-4941 . denotes .
T27439 4936-4940 NNP denotes TNFα
T27438 4930-4935 NN denotes mouse
T27437 4924-4929 JJ denotes ng/ml
T27436 4921-4923 CD denotes 10
T27435 4918-4920 CC denotes or
T27434 4909-4917 NNP denotes zVAD.fmk
T27433 4906-4908 NNP denotes µM
T27432 4903-4905 CD denotes 20
T27431 4901-4902 , denotes ,
T27430 4894-4901 NNS denotes factors
T27429 4887-4893 NN denotes growth
T27428 4884-4886 IN denotes of
T27427 4875-4883 NN denotes addition
T27426 4871-4874 DT denotes the
T27425 4868-4870 TO denotes to
T27424 4862-4867 RB denotes prior
T27423 4859-4861 NN denotes hr
T27422 4856-4858 CD denotes 24
T27421 4852-4855 IN denotes for
T27420 4844-4851 VBN denotes starved
T27419 4838-4843 JJ denotes serum
T27418 4833-4837 VBD denotes were
T27417 4827-4832 NNS denotes cells
T27416 4825-4826 , denotes ,
T27415 4815-4825 NNS denotes conditions
T27414 4810-4814 JJ denotes free
T27413 4804-4809 NN denotes serum
T27412 4798-4803 IN denotes under
T27411 4787-4797 NNS denotes treatments
T27410 4783-4786 IN denotes For
T27409 4781-4782 . denotes .
T27408 4777-4781 NNP denotes TNFα
T27407 4771-4776 NN denotes mouse
T27406 4765-4770 JJ denotes ng/ml
T27405 4762-4764 CD denotes 10
T27404 4759-4761 CC denotes or
T27403 4750-4758 NNP denotes zVAD.fmk
T27402 4747-4749 NNP denotes µM
T27401 4744-4746 CD denotes 30
T27400 4739-4743 IN denotes with
T27399 4728-4738 VBN denotes stimulated
T27398 4723-4727 VBD denotes were
T27397 4717-4722 NNS denotes cells
T27396 4715-4716 , denotes ,
T27395 4713-4715 NN denotes hr
T27394 4710-4712 CD denotes 48
T27393 4707-4709 CD denotes 24
T27392 4701-4706 IN denotes After
T27391 4699-4700 . denotes .
T27390 4693-4699 NNS denotes dishes
T27389 4689-4692 CD denotes mm2
T27388 4686-4688 CD denotes 35
T27387 4681-4685 IN denotes into
T27386 4674-4680 VBN denotes seeded
T27385 4669-4673 VBD denotes were
T27384 4667-4668 -RRB- denotes )
T27383 4662-4667 NNS denotes cells
T27382 4655-4661 NN denotes Jurkat
T27381 4651-4654 CD denotes 106
T27380 4650-4651 CD denotes ×
T27379 4649-4650 CD denotes 1
T27378 4648-4649 -LRB- denotes (
T27377 4642-4647 NNS denotes cells
T27376 4633-4641 JJ denotes adherent
T27375 4629-4632 CD denotes 105
T27374 4628-4629 CD denotes ×
T27373 4627-4628 CD denotes 4
T27372 4625-4626 , denotes ,
T27371 4621-4625 NN denotes blot
T27370 4613-4620 JJ denotes Western
T27369 4609-4612 IN denotes For
T27368 4604-4608 NN denotes Blot
T27367 4596-4603 JJ denotes Western
T26933 4049-4058 VBN denotes purchased
T26932 4044-4048 VBD denotes were
T26931 4037-4043 NNPS denotes siRNAs
T26930 4027-4036 NNP denotes Knockdown
T26929 4021-4026 NNP denotes siRNA
T26682 4009-4018 NNS denotes molecules
T26681 4003-4008 JJ denotes small
T26680 3999-4002 DT denotes the
T26679 3996-3998 IN denotes of
T26678 3987-3995 NN denotes toxicity
T26677 3974-3986 JJ denotes non-specific
T26676 3971-3973 IN denotes of
T26675 3963-3970 NNS denotes effects
T26674 3954-3962 JJ denotes possible
T26673 3950-3953 DT denotes the
T26672 3942-3949 VB denotes exclude
T26671 3939-3941 TO denotes to
T26670 3931-3938 VBN denotes plotted
T26669 3927-3930 CC denotes and
T26668 3916-3926 VBN denotes determined
T26667 3912-3915 VBD denotes was
T26666 3910-3911 , denotes ,
T26665 3905-3910 NNS denotes cells
T26664 3888-3904 JJ denotes compound-treated
T26663 3880-3887 NN denotes control
T26662 3876-3879 DT denotes the
T26661 3873-3875 TO denotes to
T26660 3864-3872 JJ denotes relative
T26659 3855-3863 NN denotes compound
T26658 3851-3854 DT denotes the
T26657 3846-3850 IN denotes with
T26656 3838-3845 VBN denotes treated
T26655 3834-3837 CC denotes and
T26654 3822-3833 NN denotes necroptosis
T26653 3814-3821 VB denotes undergo
T26652 3811-3813 TO denotes to
T26651 3803-3810 VBD denotes induced
T26650 3801-3802 , denotes ,
T26649 3796-3801 NNS denotes cells
T26648 3793-3795 IN denotes of
T26647 3783-3792 NN denotes viability
T26646 3774-3782 JJ denotes Relative
T26645 3772-3773 . denotes .
T26644 3771-3772 NN denotes %
T26643 3768-3771 CD denotes 100
T26166 2710-2711 . denotes .
T26165 2709-2710 NN denotes ′
T26164 2687-2709 NN denotes TAGTAGCGACGGGCGGTGTG-3
T26163 2686-2687 : denotes -
T26162 2685-2686 SYM denotes ′
T26161 2684-2685 CD denotes 5
T26160 2676-2683 VB denotes reverse
T26159 2674-2675 : denotes :
T26158 2671-2674 NNS denotes 18S
T26157 2665-2670 JJ denotes human
T26156 2663-2664 , denotes ,
T26155 2661-2663 CD denotes -3
T26154 2640-2660 NNP denotes CAGCCACCCGAGATTGAGCA
T26153 2638-2639 : denotes -
T26152 2637-2638 SYM denotes ′
T26151 2636-2637 CD denotes 5
T26150 2628-2635 RB denotes forward
T26149 2626-2627 : denotes :
T26148 2623-2626 NNS denotes 18S
T26147 2617-2622 JJ denotes human
T26146 2615-2616 : denotes ;
T26145 2614-2615 NN denotes ′
T26144 2590-2614 NN denotes GAGGGCTGATTAGAGAGAGGTC-3
T26143 2589-2590 : denotes -
T26142 2588-2589 SYM denotes ′
T26141 2587-2588 CD denotes 5
T26140 2579-2586 VB denotes reverse
T26139 2577-2578 : denotes :
T26138 2573-2577 NNP denotes TNFα
T26137 2567-2572 JJ denotes human
T26136 2565-2566 , denotes ,
T26135 2564-2565 NN denotes ′
T26134 2540-2564 NN denotes ATGAGCACTGAAAGCATGATCC-3
T26133 2538-2539 : denotes -
T26132 2537-2538 SYM denotes ′
T26131 2536-2537 CD denotes 5
T26130 2528-2535 RB denotes forward
T26129 2526-2527 : denotes :
T26128 2522-2526 NNP denotes TNFα
T26127 2516-2521 JJ denotes human
T26126 2515-2516 : denotes ;
T26125 2514-2515 NN denotes ′
T26124 2488-2514 NN denotes CTAAACCATCCAATCGGTAGTAGC-3
T26123 2487-2488 : denotes -
T26122 2486-2487 SYM denotes ′
T26121 2485-2486 CD denotes 5
T26120 2477-2484 VB denotes reverse
T26119 2475-2476 , denotes ,
T26118 2449-2475 NN denotes ATAACAGGTCTGTGATGCCCTTAG-3
T26117 2447-2448 NN denotes ′
T26116 2446-2447 : denotes -
T26115 2445-2446 LS denotes 5
T26114 2437-2444 RB denotes forward
T26113 2435-2436 : denotes :
T26112 2432-2435 NNS denotes 18S
T26111 2426-2431 NN denotes mouse
T26110 2425-2426 : denotes ;
T26109 2424-2425 NN denotes ′
T26108 2403-2424 NN denotes GCTACGACGTGGGCTACAG-3
T26107 2402-2403 : denotes -
T26106 2401-2402 SYM denotes ′
T26105 2400-2401 CD denotes 5
T26104 2392-2399 VB denotes reverse
T26103 2390-2391 , denotes ,
T26102 2389-2390 NN denotes ′
T26101 2364-2389 NN denotes CCCTCACACTCAGATCATCTTCT-3
T26100 2363-2364 : denotes -
T26099 2362-2363 SYM denotes ′
T26098 2361-2362 CD denotes 5
T26097 2353-2360 RB denotes forward
T26096 2351-2352 : denotes :
T26095 2347-2351 NNP denotes TNFα
T26094 2341-2346 NNP denotes Mouse
T26093 2333-2340 NNPS denotes Primers
T26092 2328-2332 NNP denotes QPCR
T25873 1920-1923 NNP denotes pAb
T25872 1913-1919 NN denotes rabbit
T25871 1911-1912 -RRB- denotes )
T25870 1905-1911 NNP denotes Ser193
T25869 1904-1905 -LRB- denotes (
T25868 1890-1903 NNP denotes phospho-FoxO4
T25867 1888-1889 , denotes ,
T25866 1885-1888 NNP denotes pAb
T25865 1878-1884 NN denotes rabbit
T25864 1876-1877 -RRB- denotes )
T25863 1873-1876 NNP denotes L27
T25862 1872-1873 -LRB- denotes (
T25861 1866-1871 NNP denotes FoxO1
T25860 1864-1865 , denotes ,
T25859 1861-1864 NNP denotes pAb
T25858 1854-1860 NN denotes rabbit
T25857 1852-1853 -RRB- denotes )
T25856 1847-1852 CD denotes Thr32
T25855 1846-1847 -LRB- denotes (
T25854 1839-1845 NNS denotes FoxO3a
T25853 1838-1839 VBP denotes /
T25852 1837-1838 -RRB- denotes )
T25851 1832-1837 NNP denotes Thr24
T25850 1831-1832 -LRB- denotes (
T25849 1817-1830 NNP denotes phospho-FoxO1
T25848 1815-1816 , denotes ,
T25847 1812-1815 NNP denotes mAb
T25846 1806-1811 NN denotes mouse
T25845 1804-1805 -RRB- denotes )
T25844 1800-1804 NNP denotes DM1A
T25843 1794-1799 JJ denotes clone
T25842 1793-1794 -LRB- denotes (
T25841 1783-1792 JJ denotes α-tubulin
T25840 1781-1782 , denotes ,
T25839 1778-1781 NNP denotes mAb
T25838 1771-1777 NN denotes rabbit
T25837 1769-1770 -RRB- denotes )
T25836 1765-1769 NNP denotes 60A8
T25835 1764-1765 -LRB- denotes (
T25834 1758-1763 NNP denotes c-Jun
T25833 1756-1757 , denotes ,
T25832 1753-1756 NNP denotes pAb
T25831 1746-1752 NN denotes rabbit
T25830 1743-1745 NNP denotes II
T25829 1741-1742 -RRB- denotes )
T25828 1736-1741 NNP denotes Ser63
T25827 1735-1736 -LRB- denotes (
T25826 1721-1734 NNP denotes phospho-c-Jun
T25825 1719-1720 , denotes ,
T25824 1716-1719 NNP denotes pAb
T25823 1709-1715 NN denotes rabbit
T25822 1700-1708 NNP denotes SAPK/JNK
T25821 1698-1699 , denotes ,
T25820 1695-1698 NNP denotes mAb
T25819 1688-1694 NN denotes rabbit
T25818 1686-1687 -RRB- denotes )
T25817 1681-1686 CD denotes 81E11
T25816 1680-1681 -LRB- denotes (
T25815 1678-1679 -RRB- denotes )
T25814 1665-1678 NNP denotes Thr183/Tyr185
T25813 1664-1665 -LRB- denotes (
T25812 1652-1663 NNP denotes phospho-JNK
T25811 1650-1651 , denotes ,
T25810 1647-1650 NNP denotes mAb
T25809 1640-1646 NN denotes rabbit
T25808 1638-1639 -RRB- denotes )
T25807 1634-1638 NNP denotes 62A8
T25806 1628-1633 VBN denotes clone
T25805 1627-1628 -LRB- denotes (
T25804 1622-1626 NNP denotes Akt3
T25803 1620-1621 , denotes ,
T25802 1617-1620 NNP denotes mAb
T25801 1610-1616 NN denotes rabbit
T25800 1608-1609 -RRB- denotes )
T25799 1604-1608 NNP denotes D6G4
T25798 1598-1603 VBN denotes clone
T25797 1597-1598 -LRB- denotes (
T25796 1592-1596 NNP denotes Akt2
T25795 1590-1591 , denotes ,
T25794 1587-1590 NNP denotes mAb
T25793 1580-1586 NN denotes rabbit
T25792 1578-1579 -RRB- denotes )
T25791 1572-1578 NNP denotes C73H10
T25790 1566-1571 VBP denotes clone
T25789 1565-1566 -LRB- denotes (
T25788 1560-1564 NNP denotes Akt1
T25787 1558-1559 , denotes ,
T25786 1555-1558 NNP denotes mAb
T25785 1548-1554 NN denotes rabbit
T25784 1546-1547 -RRB- denotes )
T25783 1541-1546 NNP denotes C67E7
T25782 1535-1540 JJ denotes clone
T25781 1534-1535 -LRB- denotes (
T25780 1532-1533 -RRB- denotes )
T25779 1529-1532 VB denotes pan
T25778 1528-1529 -LRB- denotes (
T25777 1524-1527 NNP denotes Akt
T25776 1522-1523 , denotes ,
T25775 1519-1522 NNP denotes mAb
T25774 1512-1518 NN denotes rabbit
T25773 1509-1511 NNP denotes XP
T25772 1507-1508 -RRB- denotes )
T25771 1504-1507 NNP denotes D9E
T25770 1498-1503 JJ denotes clone
T25769 1497-1498 -LRB- denotes (
T25768 1495-1496 -RRB- denotes )
T25767 1489-1495 NNP denotes Ser473
T25766 1488-1489 -LRB- denotes (
T25765 1476-1487 NNP denotes phospho-Akt
T25764 1474-1475 , denotes ,
T25763 1471-1474 NNP denotes mAb
T25762 1464-1470 NN denotes rabbit
T25761 1462-1463 -RRB- denotes )
T25760 1456-1462 NNP denotes C31E5E
T25759 1450-1455 JJ denotes clone
T25758 1449-1450 -LRB- denotes (
T25757 1447-1448 -RRB- denotes )
T25756 1441-1447 CD denotes Thr308
T25755 1440-1441 -LRB- denotes (
T25754 1428-1439 JJ denotes phospho-Akt
T25753 1426-1427 : denotes :
T25752 1422-1426 VBN denotes used
T25751 1417-1421 VBD denotes were
T25750 1406-1416 NNS denotes antibodies
T25749 1396-1405 VBG denotes following
T25748 1392-1395 DT denotes The
T25747 1381-1391 NNPS denotes Antibodies
T25719 1378-1379 . denotes .
T25718 1370-1378 NN denotes strategy
T25717 1365-1369 JJ denotes same
T25716 1361-1364 DT denotes the
T25715 1355-1360 VBG denotes using
T25714 1345-1354 VBN denotes generated
T25713 1340-1344 VBD denotes were
T25712 1331-1339 NN denotes Myr-Akt1
T25711 1328-1330 IN denotes of
T25710 1319-1327 NNS denotes versions
T25709 1312-1318 JJ denotes Mutant
T25708 1310-1311 . denotes .
T25707 1309-1310 -RRB- denotes )
T25706 1299-1309 NNP denotes Invitrogen
T25705 1298-1299 -LRB- denotes (
T25704 1291-1297 NN denotes vector
T25703 1280-1290 NN denotes retroviral
T25702 1269-1279 NN denotes pMSCV-puro
T25701 1266-1268 IN denotes of
T25700 1260-1265 NNS denotes sites
T25699 1254-1259 NNP denotes EcoRI
T25698 1250-1253 CC denotes and
T25697 1244-1249 NNP denotes BglII
T25696 1240-1243 DT denotes the
T25695 1235-1239 IN denotes into
T25694 1225-1234 VBN denotes subcloned
T25693 1221-1224 CC denotes and
T25692 1217-1220 NNP denotes PCR
T25691 1214-1216 IN denotes by
T25690 1204-1213 VBN denotes amplified
T25689 1200-1203 VBD denotes was
T25688 1186-1199 NN denotes Myr-Akt1-FLAG
T25687 1184-1185 . denotes .
T25686 1183-1184 NNP denotes ]
T25685 1181-1183 CD denotes 52
T25684 1180-1181 NNP denotes [
T26630 3691-3700 VBP denotes duplicate
T26629 3688-3690 IN denotes in
T26628 3678-3687 VBN denotes performed
T26627 3673-3677 VBD denotes were
T26626 3661-3672 NNS denotes Experiments
T26625 3659-3660 . denotes .
T26624 3658-3659 -RRB- denotes )
T26623 3651-3658 NNP denotes Promega
T26622 3650-3651 -LRB- denotes (
T26621 3644-3649 NNP denotes Assay
T26620 3634-3643 NNP denotes Viability
T26619 3629-3633 NNP denotes Cell
T26618 3615-3628 NNP denotes CellTiter-Glo
T26617 3609-3614 VBG denotes using
T26616 3598-3608 VBN denotes determined
T26615 3594-3597 VBD denotes was
T26614 3584-3593 NN denotes viability
T26613 3579-3583 NNP denotes Cell
T26612 3577-3578 . denotes .
T26611 3566-3577 NNS denotes experiments
T26610 3561-3565 NN denotes blot
T26609 3553-3560 JJ denotes western
T26608 3549-3552 IN denotes for
T26607 3539-3548 VBN denotes described
T26606 3536-3538 IN denotes as
T26605 3528-3535 VBN denotes treated
T26604 3524-3527 CC denotes and
T26603 3513-3523 NN denotes cells/well
T26602 3509-3512 CD denotes 104
T26601 3508-3509 CD denotes ×
T26600 3507-3508 CD denotes 1
T26599 3504-3506 IN denotes of
T26598 3496-3503 NN denotes density
T26597 3492-3495 DT denotes the
T26596 3489-3491 IN denotes at
T26595 3482-3488 VBZ denotes plates
T26594 3477-3481 RB denotes well
T26593 3474-3476 CD denotes 96
T26592 3467-3473 NN denotes bottom
T26591 3461-3466 JJ denotes clear
T26590 3455-3460 JJ denotes white
T26589 3450-3454 IN denotes into
T26588 3443-3449 VBN denotes seeded
T26587 3438-3442 VBD denotes were
T26586 3432-3437 NNS denotes Cells
T26585 3420-3431 NNS denotes Experiments
T26584 3410-3419 NNP denotes Viability
T26583 3405-3409 NNP denotes Cell
T26434 3262-3267 NNS denotes acids
T26433 3256-3261 JJ denotes amino
T26432 3242-3255 JJ denotes non-essential
T26431 3240-3241 , denotes ,
T26430 3229-3240 NN denotes L-glutamine
T26429 3224-3228 IN denotes with
T26428 3211-3223 VBN denotes supplemented
T26427 3198-3210 RB denotes additionally
T26426 3194-3197 VBD denotes was
T26425 3188-3193 NNS denotes media
T26424 3177-3187 NN denotes fibroblast
T26423 3172-3176 NN denotes lung
T26422 3166-3171 NN denotes mouse
T26421 3162-3165 DT denotes The
T26420 3160-3161 . denotes .
T26419 3159-3160 -RRB- denotes )
T26418 3149-3159 NNP denotes Invitrogen
T26417 3148-3149 -LRB- denotes (
T26416 3140-3147 NN denotes mixture
T26415 3117-3139 JJ denotes antibiotic-antimycotic
T26383 2955-2956 -LRB- denotes (
T26382 2950-2954 NNP denotes Yuan
T26381 2942-2949 NNP denotes Junying
T26380 2939-2941 IN denotes of
T26379 2933-2938 NNS denotes gifts
T26378 2924-2932 JJ denotes generous
T26377 2919-2923 VBD denotes were
T26376 2913-2918 NNS denotes cells
T26375 2911-2912 -RRB- denotes )
T26374 2907-2911 NNP denotes ATCC
T26373 2906-2907 -LRB- denotes (
T26354 2831-2837 NNP denotes Philip
T26353 2827-2830 NNP denotes Dr.
T26352 2824-2826 IN denotes of
T26351 2819-2823 NN denotes gift
T26350 2810-2818 JJ denotes generous
T26349 2808-2809 DT denotes a
T26348 2803-2807 VBD denotes were
T26347 2791-2802 NNS denotes fibroblasts
T26346 2786-2790 NNP denotes Lung
T26345 2784-2785 . denotes .
T26344 2780-2784 NNP denotes ATCC
T26343 2775-2779 IN denotes from
T26342 2766-2774 VBN denotes obtained
T26341 2761-2765 VBD denotes were
T26340 2755-2760 NNS denotes cells
T26339 2748-2754 NNP denotes Jurkat
T26338 2733-2747 NNP denotes FADD-deficient
T26337 2729-2732 CC denotes and
T26336 2724-2728 NNP denotes L929
T26335 2718-2723 NNP denotes Lines
T26334 2713-2717 NNP denotes Cell
T26642 3765-3767 IN denotes as
T26641 3761-3764 VBN denotes set
T26640 3757-3760 VBD denotes was
T26639 3751-3756 NNS denotes cells
T26638 3741-3750 JJ denotes untreated
T26637 3733-3740 NN denotes control
T26636 3729-3732 DT denotes the
T26635 3726-3728 IN denotes of
T26634 3716-3725 NN denotes Viability
T26633 3714-3715 . denotes .
T26632 3704-3714 VBP denotes triplicate
T26631 3701-3703 CC denotes or
T25683 1170-1179 VBN denotes described
T25682 1165-1169 VBN denotes been
T25681 1161-1164 VBZ denotes has
T25680 1159-1160 , denotes ,
T25679 1156-1159 NN denotes tag
T25678 1151-1155 NNP denotes FLAG
T25677 1140-1150 JJ denotes c-terminal
T25485 949-954 JJ denotes human
T25484 947-948 , denotes ,
T25483 946-947 -RRB- denotes )
T25482 941-946 NN denotes ng/ml
T25481 938-940 CD denotes 10
T25480 937-938 -LRB- denotes (
T25479 932-936 NNP denotes TNFα
T25478 926-931 NN denotes mouse
T25477 922-925 CC denotes and
T25476 916-921 NNP denotes Human
T25475 914-915 . denotes .
T25474 908-914 NNP denotes Bachem
T25473 903-907 IN denotes from
T25472 893-902 VBN denotes purchased
T25471 889-892 VBD denotes was
T25470 887-888 -RRB- denotes )
T25469 885-887 NNP denotes µM
T25468 882-884 CD denotes 30
T25467 879-881 CD denotes 20
T25466 878-879 -LRB- denotes (
T25465 869-877 NNP denotes zVAD.fmk
T25464 859-868 NN denotes inhibitor
T25463 847-858 NN denotes Pan-caspase
T25462 845-846 . denotes .
T25461 844-845 -RRB- denotes )
T25460 834-844 NNP denotes Calbiochem
T25459 832-833 , denotes ,
T25458 830-832 NNP denotes µM
T25457 827-829 CD denotes 50
T25456 826-827 -LRB- denotes (
T25455 819-825 NNP denotes 4EGI-1
T25454 817-818 , denotes ,
T25453 816-817 -RRB- denotes )
T25452 806-816 NNP denotes Calbiochem
T25451 804-805 , denotes ,
T25450 802-804 NNP denotes µM
T25449 799-801 CD denotes 20
T25448 798-799 -LRB- denotes (
T25447 789-797 NNP denotes PD166866
T25446 787-788 , denotes ,
T25445 786-787 -RRB- denotes )
T25444 782-786 NNP denotes Chem
T25443 775-781 NNP denotes Cayman
T25442 773-774 , denotes ,
T25441 771-773 NNP denotes µM
T25440 769-770 CD denotes 2
T25439 768-769 -LRB- denotes (
T25438 759-767 NNP denotes PD173074
T25437 757-758 , denotes ,
T25436 756-757 -RRB- denotes )
T25435 747-756 NNP denotes Signaling
T25434 742-746 NNP denotes Cell
T25433 740-741 , denotes ,
T25432 738-740 NNP denotes µM
T25431 735-737 CD denotes 10
T25430 734-735 -LRB- denotes (
T25429 725-733 NNP denotes LY249002
T25428 723-724 , denotes ,
T25427 722-723 -RRB- denotes )
T25426 716-722 NNP denotes School
T25425 708-715 NNP denotes Medical
T25424 700-707 NNP denotes Harvard
T25423 699-700 -LRB- denotes (
T25422 694-698 NNP denotes Grey
T25421 684-693 NNP denotes Nathanael
T25420 680-683 NNP denotes Dr.
T25419 677-679 IN denotes of
T25418 672-676 NN denotes gift
T25417 670-671 , denotes ,
T25416 668-670 NNP denotes nM
T25415 664-667 CD denotes 500
T25414 663-664 -LRB- denotes (
T25413 655-662 NNP denotes Torin-1
T25412 653-654 , denotes ,
T25411 652-653 -RRB- denotes )
T25410 642-652 NNP denotes Calbiochem
T25409 640-641 , denotes ,
T25408 638-640 NNP denotes µM
T25407 635-637 CD denotes 10
T25406 634-635 -LRB- denotes (
T25405 627-633 NN denotes PI-103
T25404 625-626 , denotes ,
T25403 624-625 -RRB- denotes )
T25402 620-624 NNP denotes Cruz
T25401 614-619 NNP denotes Santa
T25400 612-613 , denotes ,
T25399 610-612 NNP denotes nM
T25398 606-609 CD denotes 100
T25397 605-606 -LRB- denotes (
T25396 595-604 NN denotes rapamycin
T25395 593-594 , denotes ,
T25394 592-593 -RRB- denotes )
T25393 587-592 NNP denotes Sigma
T25392 586-587 -LRB- denotes (
T25391 575-585 NNP denotes PF-4706871
T25390 573-574 , denotes ,
T25389 572-573 -RRB- denotes )
T25388 568-572 NNP denotes Chem
T25387 564-567 NNP denotes Med
T25386 559-563 NNP denotes Axon
T25385 557-558 , denotes ,
T25384 555-557 NNP denotes µM
T26414 3115-3116 NN denotes %
T26413 3114-3115 CD denotes 1
T26412 3110-3113 CC denotes and
T26411 3108-3109 -RRB- denotes )
T26410 3105-3108 NNP denotes FBS
T26409 3104-3105 -LRB- denotes (
T26408 3098-3103 NN denotes serum
T26407 3091-3097 JJ denotes bovine
T26406 3085-3090 JJ denotes fetal
T26405 3083-3084 NN denotes %
T26404 3081-3083 CD denotes 10
T26403 3076-3080 IN denotes with
T26402 3063-3075 VBN denotes supplemented
T26401 3058-3062 NNP denotes DMEM
T26400 3055-3057 IN denotes in
T26399 3044-3054 VBN denotes maintained
T26398 3039-3043 VBD denotes were
T26397 3033-3038 NNS denotes Cells
T26396 3031-3032 . denotes .
T26395 3019-3031 RB denotes respectively
T26394 3017-3018 , denotes ,
T26393 3016-3017 -RRB- denotes )
T26392 3006-3016 NNP denotes University
T26391 3000-3005 NNP denotes Tufts
T26390 2999-3000 -LRB- denotes (
T26389 2990-2998 NNP denotes Poltorak
T26388 2980-2989 NNP denotes Alexander
T26387 2976-2979 CC denotes and
T26386 2974-2975 -RRB- denotes )
T26385 2964-2974 NNP denotes University
T26384 2956-2963 NNP denotes Harvard
T25964 2325-2326 . denotes .
T25963 2324-2325 -RRB- denotes )
T25962 2315-2324 NNP denotes Bioworlde
T25961 2314-2315 -LRB- denotes (
T25960 2310-2313 NNP denotes pAb
T25959 2303-2309 NN denotes rabbit
T25958 2298-2302 NNP denotes MDM2
T25957 2296-2297 , denotes ,
T25956 2295-2296 -RRB- denotes )
T25955 2286-2295 NNP denotes Signaling
T25954 2281-2285 NNP denotes Cell
T25953 2277-2280 DT denotes all
T25952 2276-2277 -LRB- denotes (
T25951 2272-2275 NNP denotes pAb
T25950 2265-2271 NN denotes rabbit
T25949 2260-2264 NNP denotes PDK1
T25948 2258-2259 , denotes ,
T25947 2255-2258 NNP denotes mAb
T25946 2248-2254 NN denotes rabbit
T25945 2246-2247 -RRB- denotes )
T25944 2242-2246 NNP denotes 7C10
T25943 2236-2241 VBP denotes clone
T25942 2235-2236 -LRB- denotes (
T25941 2230-2234 NNP denotes mTOR
T25940 2228-2229 , denotes ,
T25939 2225-2228 NNP denotes pAb
T25938 2218-2224 NN denotes rabbit
T25937 2216-2217 -RRB- denotes )
T25936 2208-2216 NNP denotes Thr37/46
T25935 2207-2208 -LRB- denotes (
T25934 2192-2206 NNP denotes phospho-4E-BP1
T25933 2190-2191 , denotes ,
T25932 2187-2190 NNP denotes mAb
T25931 2181-2186 NN denotes mouse
T25930 2179-2180 -RRB- denotes )
T25929 2175-2179 NNP denotes 54D2
T25928 2169-2174 VBN denotes clone
T25927 2168-2169 -LRB- denotes (
T25926 2160-2167 NNP denotes Protein
T25925 2150-2159 NNP denotes Ribosomal
T25924 2147-2149 NNP denotes S6
T25923 2145-2146 , denotes ,
T25922 2142-2145 NNP denotes mAb
T25921 2135-2141 NN denotes rabbit
T25920 2132-2134 NNP denotes XP
T25919 2130-2131 -RRB- denotes )
T25918 2129-2130 NNP denotes E
T25917 2125-2129 CD denotes .2.2
T25916 2122-2125 NNP denotes D57
T25915 2116-2121 JJ denotes clone
T25914 2115-2116 -LRB- denotes (
T25913 2113-2114 -RRB- denotes )
T25912 2103-2113 NNP denotes Ser235/236
T25911 2102-2103 -LRB- denotes (
T25910 2094-2101 NNP denotes Protein
T25909 2084-2093 NNP denotes Ribosomal
T25908 2073-2083 NNP denotes phospho-S6
T25907 2071-2072 , denotes ,
T25906 2068-2071 NNP denotes mAb
T25905 2061-2067 NN denotes rabbit
T25904 2059-2060 -RRB- denotes )
T25903 2054-2059 NNP denotes 108D2
T25902 2048-2053 JJ denotes clone
T25901 2047-2048 -LRB- denotes (
T25900 2045-2046 -RRB- denotes )
T25899 2039-2045 NNP denotes Thr389
T25898 2038-2039 -LRB- denotes (
T25897 2031-2037 NNP denotes Kinase
T25896 2028-2030 NNP denotes S6
T25895 2016-2027 JJ denotes phospho-p70
T25894 2014-2015 , denotes ,
T25893 2011-2014 NNP denotes pAb
T25892 2004-2010 NN denotes rabbit
T25891 2002-2003 -RRB- denotes )
T25890 1995-2002 NNP denotes Ser21/9
T25889 1994-1995 -LRB- denotes (
T25888 1992-1993 NNP denotes β
T25887 1991-1992 NNP denotes /
T25886 1977-1991 NNP denotes phospho-GSK-3α
T25885 1975-1976 , denotes ,
T25884 1972-1975 NNP denotes pAb
T25883 1965-1971 NN denotes rabbit
T25882 1963-1964 -RRB- denotes )
T25881 1957-1963 NNP denotes Ser166
T25880 1956-1957 -LRB- denotes (
T25879 1943-1955 NNP denotes phospho-MDM2
T25878 1941-1942 , denotes ,
T25877 1938-1941 NNP denotes pAb
T25876 1931-1937 NN denotes rabbit
T25875 1925-1930 NNP denotes FoxO4
T25874 1923-1924 , denotes ,
T25383 552-554 CD denotes 10
T25382 551-552 -LRB- denotes (
T25381 545-550 NNP denotes BX912
T25380 543-544 , denotes ,
T25379 542-543 -RRB- denotes )
T25378 532-542 NNP denotes Calbiochem
T25377 530-531 , denotes ,
T25376 528-530 NNP denotes µM
T25375 525-527 CD denotes 10
T25374 524-525 -LRB- denotes (
T25373 515-523 NNP denotes SB216763
T25372 513-514 , denotes ,
T25371 512-513 -RRB- denotes )
T25370 507-512 NNP denotes Sigma
T25369 505-506 , denotes ,
T25368 503-505 NNP denotes mM
T25367 500-502 CD denotes 10
T25366 499-500 -LRB- denotes (
T25365 494-498 NNP denotes LiCl
T25364 492-493 , denotes ,
T25363 491-492 -RRB- denotes )
T25362 481-491 NNP denotes Calbiochem
T25361 479-480 , denotes ,
T25360 477-479 NNP denotes µM
T25359 474-476 CD denotes 10
T25337 394-395 , denotes ,
T25336 393-394 -RRB- denotes )
T25335 383-393 NNP denotes Calbiochem
T25334 381-382 , denotes ,
T25333 379-381 NNP denotes µM
T25332 376-378 CD denotes 10
T25331 375-376 -LRB- denotes (
T25330 366-374 NNP denotes SP600125
T25329 364-365 , denotes ,
T25328 363-364 -RRB- denotes )
T25327 354-363 NNP denotes Institute
T25326 347-353 NNP denotes Cancer
T25325 338-346 NNP denotes National
T25324 336-337 , denotes ,
T25323 334-336 NNP denotes µM
T25322 330-333 CD denotes 100
T25321 329-330 -LRB- denotes (
T25320 317-328 NNP denotes Triciribine
T25319 315-316 , denotes ,
T25318 314-315 -RRB- denotes )
T25317 310-314 NNP denotes Chem
T25316 302-309 NNP denotes Selleck
T25315 300-301 , denotes ,
T25314 298-300 NNP denotes µM
T25313 295-297 CD denotes 10
T25312 294-295 -LRB- denotes (
T25311 286-293 NNP denotes MK-2206
T25310 284-285 , denotes ,
T25309 283-284 -RRB- denotes )
T25308 273-283 NNP denotes Calbiochem
T25280 117-118 . denotes .
T25279 116-117 NNP denotes ]
T25278 114-116 CD denotes 24
T25277 113-114 NNP denotes [
T25276 111-112 , denotes ,
T25275 110-111 NNP denotes ]
T25274 108-110 CD denotes 23
T25273 107-108 NNP denotes [
T25272 97-106 VBN denotes described
T25271 86-96 RB denotes previously
T25270 83-85 IN denotes as
T25269 71-82 VBN denotes synthesized
T25268 66-70 VBD denotes were
T25267 58-65 NNS denotes analogs
T25266 46-57 NNP denotes Necrostatin
T25265 36-45 NNPS denotes Chemicals
T25264 32-35 CC denotes and
T25263 23-31 NNS denotes Reagents
T25344 421-422 , denotes ,
T25343 419-421 NNP denotes µM
T25342 416-418 CD denotes 10
T25341 415-416 -LRB- denotes (
T25340 410-414 NNP denotes VIII
T25339 400-409 NN denotes inhibitor
T25338 396-399 NNP denotes JNK
T25307 271-272 , denotes ,
T25306 269-271 NNP denotes µM
T25305 266-268 CD denotes 10
T25304 265-266 -LRB- denotes (
T25303 260-264 NNP denotes VIII
T25302 250-259 NN denotes inhibitor
T25301 246-249 JJ denotes Akt
T25300 244-245 : denotes :
T25299 233-244 NNS denotes experiments
T25298 229-232 DT denotes the
T25297 226-228 IN denotes in
T25296 221-225 VBN denotes used
T25295 216-220 VBD denotes were
T25294 214-215 -RRB- denotes )
T25293 202-214 NNS denotes text/figures
T25292 198-201 DT denotes the
T25291 195-197 IN denotes in
T25290 185-194 VBN denotes specified
T25289 175-184 RB denotes otherwise
T25288 168-174 IN denotes unless
T25287 167-168 -LRB- denotes (
T25286 152-166 NNS denotes concentrations
T25285 146-151 JJ denotes final
T25284 142-145 CC denotes and
T25283 133-141 NNS denotes reagents
T25282 123-132 VBG denotes following
T25281 119-122 DT denotes The
T25358 473-474 -LRB- denotes (
T25357 464-472 NNP denotes PD169316
T25356 462-463 , denotes ,
T25355 461-462 -RRB- denotes )
T25354 457-461 NNP denotes Chem
T25353 450-456 NNP denotes Cayman
T25352 448-449 , denotes ,
T25351 446-448 NNP denotes mM
T25350 443-445 CD denotes 10
T25349 442-443 -LRB- denotes (
T25348 436-441 NNP denotes UO126
T25347 434-435 , denotes ,
T25346 433-434 -RRB- denotes )
T25345 423-433 NNP denotes Calbiochem
T25523 1101-1102 . denotes .
T25522 1096-1101 NNP denotes Sigma
T25521 1091-1095 IN denotes from
T25520 1086-1090 VBD denotes were
T25519 1077-1085 NNS denotes reagents
T25518 1071-1076 JJ denotes other
T25517 1067-1070 DT denotes All
T25516 1065-1066 . denotes .
T25515 1056-1065 NNP denotes Peprotech
T25514 1053-1055 CC denotes or
T25513 1044-1052 NNPS denotes Sciences
T25512 1039-1043 NNP denotes Cell
T25511 1034-1038 IN denotes from
T25510 1029-1033 VBD denotes were
T25509 1027-1028 -RRB- denotes )
T25508 1022-1027 NN denotes ng/ml
T25507 1019-1021 CD denotes 50
T25506 1018-1019 -LRB- denotes (
T25505 1012-1017 NNP denotes IGF-1
T25504 1008-1011 CC denotes and
T25503 1006-1007 , denotes ,
T25502 1005-1006 -RRB- denotes )
T25501 1000-1005 NN denotes ng/ml
T25500 997-999 CD denotes 20
T25499 996-997 -LRB- denotes (
T25498 988-995 NNP denotes PDGF-BB
T25497 986-987 , denotes ,
T25496 985-986 -RRB- denotes )
T25495 980-985 NN denotes ng/ml
T25494 977-979 CD denotes 50
T25493 976-977 -LRB- denotes (
T25492 972-975 NNP denotes EGF
T25491 970-971 , denotes ,
T25490 969-970 -RRB- denotes )
T25489 964-969 NN denotes ng/ml
T25488 961-963 CD denotes 25
T25487 960-961 -LRB- denotes (
T25486 955-959 NNP denotes bFGF
T26372 2903-2905 CD denotes .7
T26371 2897-2903 NNP denotes RAW264
T26370 2893-2896 CC denotes and
T26369 2887-2892 NNS denotes cells
T26368 2885-2886 -RRB- denotes )
T26367 2881-2885 NNP denotes ATCC
T26366 2880-2881 -LRB- denotes (
T26365 2877-2879 CD denotes .1
T26364 2872-2877 CD denotes J774A
T26363 2870-2871 . denotes .
T26362 2869-2870 NNP denotes ]
T26361 2867-2869 CD denotes 53
T26360 2866-2867 NNP denotes [
T26359 2864-2865 -RRB- denotes )
T26358 2854-2864 NNP denotes University
T26357 2848-2853 NNP denotes Tufts
T26356 2847-2848 -LRB- denotes (
T26355 2838-2846 NNP denotes Tsichlis
T26459 3402-3403 . denotes .
T26458 3380-3402 JJ denotes antibiotic-antimycotic
T26457 3378-3379 NN denotes %
T26456 3377-3378 CD denotes 1
T26455 3373-3376 CC denotes and
T26454 3371-3372 -RRB- denotes )
T26453 3365-3371 NNP denotes Gemini
T26452 3364-3365 -LRB- denotes (
T26451 3354-3363 NNP denotes FetalPlex
T26450 3352-3353 NN denotes %
T26449 3350-3352 CD denotes 10
T26448 3345-3349 IN denotes with
T26447 3332-3344 VBN denotes supplemented
T26446 3330-3331 , denotes ,
T26445 3322-3330 NNP denotes RPMI1640
T26444 3319-3321 IN denotes in
T26443 3308-3318 VBN denotes maintained
T26442 3303-3307 VBD denotes were
T26441 3297-3302 NNS denotes cells
T26440 3290-3296 NNP denotes Jurkat
T26439 3288-3289 . denotes .
T26438 3280-3288 NN denotes pyruvate
T26437 3273-3279 NN denotes sodium
T26436 3269-3272 CC denotes and
T26435 3267-3268 , denotes ,
T25676 1129-1139 VBG denotes containing
T25675 1127-1128 , denotes ,
T25674 1119-1127 NNP denotes Myr-Akt1
T25673 1116-1118 IN denotes of
T25672 1108-1115 NNP denotes Cloning
T25671 1104-1107 NNP denotes DNA
T28115 6917-6925 NNP denotes ELISAOne
T28114 6914-6916 IN denotes of
T28113 6911-6913 NN denotes µL
T28112 6908-6910 CD denotes 45
T28111 6905-6907 IN denotes in
T28110 6897-6904 VBN denotes diluted
T28109 6892-6896 VBD denotes were
T28108 6884-6891 NNS denotes samples
R17874 T26140 T26160 ccomp reverse,reverse
R17875 T26141 T26140 nummod 5,reverse
R17881 T26147 T26148 amod human,18S
R17826 T26092 T26095 compound QPCR,TNFα
R17197 T25263 T25269 nsubjpass Reagents,synthesized
R17198 T25264 T25263 cc and,Reagents
R17199 T25265 T25266 compound Chemicals,Necrostatin
R17200 T25266 T25267 compound Necrostatin,analogs
R17201 T25267 T25263 conj analogs,Reagents
R17202 T25268 T25269 auxpass were,synthesized
R17203 T25269 T25269 ROOT synthesized,synthesized
R17204 T25270 T25272 mark as,described
R17205 T25271 T25272 advmod previously,described
R17206 T25272 T25269 advcl described,synthesized
R17207 T25273 T25275 compound [,]
R17208 T25274 T25275 compound 23,]
R17209 T25275 T25272 dobj ],described
R17210 T25276 T25275 punct ",",]
R17211 T25277 T25279 nmod [,]
R17212 T25278 T25279 nummod 24,]
R17213 T25279 T25275 appos ],]
R17214 T25280 T25269 punct .,synthesized
R17215 T25281 T25283 det The,reagents
R17216 T25282 T25283 amod following,reagents
R17217 T25283 T25296 nsubjpass reagents,used
R17218 T25284 T25283 cc and,reagents
R17219 T25285 T25286 amod final,concentrations
R17220 T25286 T25283 conj concentrations,reagents
R17221 T25287 T25290 punct (,specified
R17222 T25288 T25290 mark unless,specified
R17223 T25289 T25290 advmod otherwise,specified
R17224 T25290 T25283 acl specified,reagents
R17225 T25291 T25290 prep in,specified
R17226 T25292 T25293 det the,text/figures
R17227 T25293 T25291 pobj text/figures,in
R17228 T25294 T25283 punct ),reagents
R17229 T25295 T25296 auxpass were,used
R17230 T25296 T25296 ROOT used,used
R17231 T25297 T25296 prep in,used
R17232 T25298 T25299 det the,experiments
R17233 T25299 T25297 pobj experiments,in
R17234 T25300 T25303 punct :,VIII
R17235 T25301 T25303 amod Akt,VIII
R17236 T25302 T25303 compound inhibitor,VIII
R17237 T25303 T25296 npadvmod VIII,used
R17238 T25304 T25303 punct (,VIII
R17239 T25305 T25306 nummod 10,µM
R17240 T25306 T25303 appos µM,VIII
R17241 T25307 T25306 punct ",",µM
R17242 T25308 T25306 appos Calbiochem,µM
R17243 T25309 T25306 punct ),µM
R17244 T25310 T25303 punct ",",VIII
R17245 T25311 T25314 nmod MK-2206,µM
R17246 T25312 T25314 punct (,µM
R17247 T25313 T25314 nummod 10,µM
R17248 T25314 T25303 appos µM,VIII
R17249 T25315 T25314 punct ",",µM
R17250 T25316 T25317 compound Selleck,Chem
R17251 T25317 T25314 appos Chem,µM
R17252 T25318 T25314 punct ),µM
R17253 T25319 T25314 punct ",",µM
R17254 T25320 T25314 conj Triciribine,µM
R17255 T25321 T25323 punct (,µM
R17256 T25322 T25323 nummod 100,µM
R17257 T25323 T25314 conj µM,µM
R17258 T25324 T25323 punct ",",µM
R17259 T25325 T25327 compound National,Institute
R17260 T25326 T25327 compound Cancer,Institute
R17261 T25327 T25323 appos Institute,µM
R17262 T25328 T25323 punct ),µM
R17263 T25329 T25323 punct ",",µM
R17264 T25330 T25323 conj SP600125,µM
R17265 T25331 T25333 punct (,µM
R17266 T25332 T25333 nummod 10,µM
R17267 T25333 T25323 conj µM,µM
R17268 T25334 T25333 punct ",",µM
R17269 T25335 T25333 conj Calbiochem,µM
R17270 T25336 T25335 punct ),Calbiochem
R17271 T25337 T25333 punct ",",µM
R17272 T25338 T25340 compound JNK,VIII
R17273 T25339 T25340 compound inhibitor,VIII
R17274 T25340 T25333 npadvmod VIII,µM
R17275 T25341 T25340 punct (,VIII
R17276 T25342 T25343 nummod 10,µM
R17277 T25343 T25340 appos µM,VIII
R17278 T25344 T25340 punct ",",VIII
R17279 T25345 T25340 appos Calbiochem,VIII
R17280 T25346 T25340 punct ),VIII
R17281 T25347 T25340 punct ",",VIII
R17282 T25348 T25340 appos UO126,VIII
R17283 T25349 T25351 punct (,mM
R17284 T25350 T25351 nummod 10,mM
R17285 T25351 T25340 appos mM,VIII
R17286 T25352 T25351 punct ",",mM
R17287 T25353 T25354 compound Cayman,Chem
R17288 T25354 T25351 npadvmod Chem,mM
R17289 T25355 T25351 punct ),mM
R17290 T25356 T25340 punct ",",VIII
R17291 T25357 T25340 appos PD169316,VIII
R17292 T25358 T25360 punct (,µM
R17293 T25359 T25360 nummod 10,µM
R17294 T25360 T25340 appos µM,VIII
R17295 T25361 T25360 punct ",",µM
R17296 T25362 T25360 appos Calbiochem,µM
R17297 T25363 T25360 punct ),µM
R17298 T25364 T25360 punct ",",µM
R17299 T25365 T25360 conj LiCl,µM
R17300 T25366 T25368 punct (,mM
R17301 T25367 T25368 nummod 10,mM
R17302 T25368 T25340 appos mM,VIII
R17303 T25369 T25368 punct ",",mM
R17304 T25370 T25368 npadvmod Sigma,mM
R17305 T25371 T25368 punct ),mM
R17306 T25372 T25340 punct ",",VIII
R17307 T25373 T25340 appos SB216763,VIII
R17308 T25374 T25376 punct (,µM
R17309 T25375 T25376 nummod 10,µM
R17310 T25376 T25340 appos µM,VIII
R17311 T25377 T25376 punct ",",µM
R17312 T25378 T25376 appos Calbiochem,µM
R17313 T25379 T25376 punct ),µM
R17314 T25380 T25376 punct ",",µM
R17315 T25381 T25376 conj BX912,µM
R17316 T25382 T25384 punct (,µM
R17317 T25383 T25384 nummod 10,µM
R17318 T25384 T25388 npadvmod µM,Chem
R17319 T25385 T25384 punct ",",µM
R17320 T25386 T25388 compound Axon,Chem
R17321 T25387 T25388 compound Med,Chem
R17322 T25388 T25340 appos Chem,VIII
R17323 T25389 T25340 punct ),VIII
R17324 T25390 T25340 punct ",",VIII
R17325 T25391 T25340 appos PF-4706871,VIII
R17326 T25392 T25393 punct (,Sigma
R17327 T25393 T25391 parataxis Sigma,PF-4706871
R17328 T25394 T25393 punct ),Sigma
R17329 T25395 T25340 punct ",",VIII
R17330 T25396 T25399 nmod rapamycin,nM
R17331 T25397 T25399 punct (,nM
R17332 T25398 T25399 nummod 100,nM
R17333 T25399 T25340 appos nM,VIII
R17334 T25400 T25399 punct ",",nM
R17335 T25401 T25402 compound Santa,Cruz
R17336 T25402 T25399 appos Cruz,nM
R17337 T25403 T25399 punct ),nM
R17338 T25404 T25399 punct ",",nM
R17339 T25405 T25408 nmod PI-103,µM
R17340 T25406 T25408 punct (,µM
R17341 T25407 T25408 nummod 10,µM
R17342 T25408 T25399 conj µM,nM
R17343 T25409 T25408 punct ",",µM
R17344 T25410 T25408 conj Calbiochem,µM
R17345 T25411 T25410 punct ),Calbiochem
R17346 T25412 T25408 punct ",",µM
R17347 T25413 T25408 conj Torin-1,µM
R17348 T25414 T25416 punct (,nM
R17349 T25415 T25416 nummod 500,nM
R17350 T25416 T25408 conj nM,µM
R17351 T25417 T25416 punct ",",nM
R17352 T25418 T25340 appos gift,VIII
R17353 T25419 T25418 prep of,gift
R17354 T25420 T25422 compound Dr.,Grey
R17355 T25421 T25422 compound Nathanael,Grey
R17356 T25422 T25419 pobj Grey,of
R17357 T25423 T25426 punct (,School
R17358 T25424 T25426 compound Harvard,School
R17359 T25425 T25426 compound Medical,School
R17360 T25426 T25422 appos School,Grey
R17361 T25427 T25426 punct ),School
R17362 T25428 T25422 punct ",",Grey
R17363 T25429 T25432 nmod LY249002,µM
R17364 T25430 T25432 punct (,µM
R17365 T25431 T25432 nummod 10,µM
R17366 T25432 T25422 appos µM,Grey
R17367 T25433 T25432 punct ",",µM
R17368 T25434 T25435 compound Cell,Signaling
R17369 T25435 T25432 conj Signaling,µM
R17370 T25436 T25432 punct ),µM
R17371 T25437 T25432 punct ",",µM
R17372 T25438 T25432 conj PD173074,µM
R17373 T25439 T25441 punct (,µM
R17374 T25440 T25441 nummod 2,µM
R17375 T25441 T25432 conj µM,µM
R17376 T25442 T25441 punct ",",µM
R17377 T25443 T25444 compound Cayman,Chem
R17378 T25444 T25441 npadvmod Chem,µM
R17379 T25445 T25441 punct ),µM
R17380 T25446 T25441 punct ",",µM
R17381 T25447 T25441 conj PD166866,µM
R17382 T25448 T25450 punct (,µM
R17383 T25449 T25450 nummod 20,µM
R17384 T25450 T25441 conj µM,µM
R17385 T25451 T25450 punct ",",µM
R17386 T25452 T25450 conj Calbiochem,µM
R17387 T25453 T25452 punct ),Calbiochem
R17388 T25454 T25450 punct ",",µM
R17389 T25455 T25450 conj 4EGI-1,µM
R17390 T25456 T25458 punct (,µM
R17391 T25457 T25458 nummod 50,µM
R17392 T25458 T25450 conj µM,µM
R17393 T25459 T25458 punct ",",µM
R17394 T25460 T25458 conj Calbiochem,µM
R17395 T25461 T25458 punct ),µM
R17396 T25462 T25303 punct .,VIII
R17397 T25463 T25465 compound Pan-caspase,zVAD.fmk
R17398 T25464 T25465 compound inhibitor,zVAD.fmk
R17399 T25465 T25472 nsubjpass zVAD.fmk,purchased
R17400 T25466 T25465 punct (,zVAD.fmk
R17401 T25467 T25465 appos 20,zVAD.fmk
R17402 T25468 T25469 nummod 30,µM
R17403 T25469 T25465 appos µM,zVAD.fmk
R17404 T25470 T25465 punct ),zVAD.fmk
R17405 T25471 T25472 auxpass was,purchased
R17406 T25472 T25472 ROOT purchased,purchased
R17407 T25473 T25472 prep from,purchased
R17408 T25474 T25473 pobj Bachem,from
R17409 T25475 T25472 punct .,purchased
R17410 T25476 T25510 nsubj Human,were
R17411 T25477 T25476 cc and,Human
R17412 T25478 T25476 conj mouse,Human
R17413 T25479 T25478 appos TNFα,mouse
R17414 T25480 T25482 punct (,ng/ml
R17415 T25481 T25482 nummod 10,ng/ml
R17416 T25482 T25479 appos ng/ml,TNFα
R17417 T25483 T25482 punct ),ng/ml
R17418 T25484 T25479 punct ",",TNFα
R17419 T25485 T25486 compound human,bFGF
R17420 T25486 T25479 conj bFGF,TNFα
R17421 T25487 T25489 punct (,ng/ml
R17422 T25488 T25489 nummod 25,ng/ml
R17423 T25489 T25486 appos ng/ml,bFGF
R17424 T25490 T25489 punct ),ng/ml
R17425 T25491 T25486 punct ",",bFGF
R17426 T25492 T25486 conj EGF,bFGF
R17427 T25493 T25495 punct (,ng/ml
R17428 T25494 T25495 nummod 50,ng/ml
R17429 T25495 T25492 parataxis ng/ml,EGF
R17430 T25496 T25495 punct ),ng/ml
R17431 T25497 T25492 punct ",",EGF
R17432 T25498 T25492 conj PDGF-BB,EGF
R17433 T25499 T25501 punct (,ng/ml
R17434 T25500 T25501 nummod 20,ng/ml
R17435 T25501 T25498 parataxis ng/ml,PDGF-BB
R17436 T25502 T25501 punct ),ng/ml
R17437 T25503 T25498 punct ",",PDGF-BB
R17438 T25504 T25498 cc and,PDGF-BB
R17439 T25505 T25498 conj IGF-1,PDGF-BB
R17440 T25506 T25508 punct (,ng/ml
R17441 T25507 T25508 nummod 50,ng/ml
R17442 T25508 T25505 appos ng/ml,IGF-1
R17443 T25509 T25505 punct ),IGF-1
R17444 T25510 T25510 ROOT were,were
R17445 T25511 T25510 prep from,were
R17446 T25512 T25513 compound Cell,Sciences
R17447 T25513 T25511 pobj Sciences,from
R17448 T25514 T25513 cc or,Sciences
R17449 T25515 T25513 conj Peprotech,Sciences
R17450 T25516 T25510 punct .,were
R17451 T25517 T25519 det All,reagents
R17452 T25518 T25519 amod other,reagents
R17453 T25519 T25520 nsubj reagents,were
R17454 T25520 T25520 ROOT were,were
R17455 T25521 T25520 prep from,were
R17456 T25522 T25521 pobj Sigma,from
R17457 T25523 T25520 punct .,were
R17533 T25691 T25690 agent by,amplified
R17534 T25692 T25691 pobj PCR,by
R17535 T25693 T25690 cc and,amplified
R17536 T25694 T25690 conj subcloned,amplified
R17537 T25695 T25694 prep into,subcloned
R17538 T25696 T25697 det the,BglII
R17539 T25697 T25700 nmod BglII,sites
R17540 T25698 T25697 cc and,BglII
R17541 T25699 T25697 conj EcoRI,BglII
R17542 T25700 T25695 pobj sites,into
R17543 T25701 T25700 prep of,sites
R17544 T25702 T25704 compound pMSCV-puro,vector
R17545 T25703 T25704 compound retroviral,vector
R17546 T25704 T25701 pobj vector,of
R17547 T25705 T25704 punct (,vector
R17548 T25706 T25704 appos Invitrogen,vector
R17549 T25707 T25704 punct ),vector
R17550 T25708 T25690 punct .,amplified
R17551 T25709 T25710 amod Mutant,versions
R17552 T25710 T25714 nsubjpass versions,generated
R17553 T25711 T25710 prep of,versions
R17554 T25712 T25711 pobj Myr-Akt1,of
R17555 T25713 T25714 auxpass were,generated
R17556 T25714 T25714 ROOT generated,generated
R17557 T25715 T25714 advcl using,generated
R17558 T25716 T25718 det the,strategy
R17559 T25717 T25718 amod same,strategy
R17560 T25718 T25715 dobj strategy,using
R17561 T25719 T25714 punct .,generated
R17562 T25747 T25752 nsubjpass Antibodies,used
R17563 T25748 T25750 det The,antibodies
R17564 T25749 T25750 amod following,antibodies
R17565 T25750 T25752 nsubjpass antibodies,used
R17566 T25751 T25752 auxpass were,used
R17567 T25752 T25752 ROOT used,used
R17568 T25753 T25775 punct :,mAb
R17569 T25754 T25774 meta phospho-Akt,rabbit
R17570 T25755 T25754 punct (,phospho-Akt
R17571 T25756 T25754 dep Thr308,phospho-Akt
R17572 T25757 T25754 punct ),phospho-Akt
R17573 T25758 T25760 punct (,C31E5E
R17574 T25759 T25760 compound clone,C31E5E
R17575 T25760 T25754 appos C31E5E,phospho-Akt
R17576 T25761 T25760 punct ),C31E5E
R17577 T25762 T25763 compound rabbit,mAb
R17578 T25763 T25754 conj mAb,phospho-Akt
R17579 T25764 T25763 punct ",",mAb
R17580 T25765 T25763 conj phospho-Akt,mAb
R17581 T25766 T25765 punct (,phospho-Akt
R17582 T25767 T25765 appos Ser473,phospho-Akt
R17583 T25768 T25765 punct ),phospho-Akt
R17584 T25769 T25771 punct (,D9E
R17585 T25770 T25771 compound clone,D9E
R17586 T25771 T25765 appos D9E,phospho-Akt
R17587 T25772 T25771 punct ),D9E
R17588 T25773 T25774 compound XP,rabbit
R17589 T25774 T25775 compound rabbit,mAb
R17590 T25775 T25752 dobj mAb,used
R17591 T25776 T25775 punct ",",mAb
R17592 T25777 T25775 conj Akt,mAb
R17593 T25778 T25779 punct (,pan
R17594 T25779 T25777 parataxis pan,Akt
R17595 T25780 T25779 punct ),pan
R17596 T25781 T25783 punct (,C67E7
R17597 T25782 T25783 compound clone,C67E7
R17598 T25783 T25777 appos C67E7,Akt
R17599 T25784 T25783 punct ),C67E7
R17600 T25785 T25786 compound rabbit,mAb
R17601 T25786 T25775 conj mAb,mAb
R17602 T25787 T25786 punct ",",mAb
R17603 T25788 T25791 compound Akt1,C73H10
R17604 T25789 T25790 punct (,clone
R17605 T25790 T25791 compound clone,C73H10
R17606 T25791 T25786 appos C73H10,mAb
R17607 T25792 T25786 punct ),mAb
R17608 T25793 T25794 compound rabbit,mAb
R17609 T25794 T25786 conj mAb,mAb
R17610 T25795 T25794 punct ",",mAb
R17611 T25796 T25799 compound Akt2,D6G4
R17612 T25797 T25798 punct (,clone
R17613 T25798 T25799 compound clone,D6G4
R17614 T25799 T25794 appos D6G4,mAb
R17615 T25800 T25794 punct ),mAb
R17616 T25801 T25802 compound rabbit,mAb
R17617 T25802 T25794 conj mAb,mAb
R17618 T25803 T25802 punct ",",mAb
R17619 T25804 T25807 compound Akt3,62A8
R17620 T25805 T25806 punct (,clone
R17621 T25806 T25807 compound clone,62A8
R17622 T25807 T25802 appos 62A8,mAb
R17623 T25808 T25802 punct ),mAb
R17624 T25809 T25810 compound rabbit,mAb
R17625 T25810 T25802 conj mAb,mAb
R17626 T25811 T25810 punct ",",mAb
R17627 T25812 T25810 conj phospho-JNK,mAb
R17628 T25813 T25812 punct (,phospho-JNK
R17629 T25814 T25812 appos Thr183/Tyr185,phospho-JNK
R17630 T25815 T25812 punct ),phospho-JNK
R17631 T25816 T25812 punct (,phospho-JNK
R17632 T25817 T25812 appos 81E11,phospho-JNK
R17633 T25818 T25812 punct ),phospho-JNK
R17634 T25819 T25820 compound rabbit,mAb
R17635 T25820 T25810 conj mAb,mAb
R17636 T25821 T25820 punct ",",mAb
R17637 T25822 T25823 compound SAPK/JNK,rabbit
R17638 T25823 T25824 compound rabbit,pAb
R17639 T25824 T25820 conj pAb,mAb
R17640 T25825 T25824 punct ",",pAb
R17641 T25826 T25831 nmod phospho-c-Jun,rabbit
R17642 T25827 T25828 punct (,Ser63
R17643 T25828 T25826 appos Ser63,phospho-c-Jun
R17644 T25829 T25828 punct ),Ser63
R17645 T25830 T25831 compound II,rabbit
R17646 T25831 T25832 compound rabbit,pAb
R17647 T25832 T25824 conj pAb,pAb
R17648 T25833 T25832 punct ",",pAb
R17649 T25834 T25832 conj c-Jun,pAb
R17650 T25835 T25836 punct (,60A8
R17651 T25836 T25834 appos 60A8,c-Jun
R17652 T25837 T25834 punct ),c-Jun
R17653 T25838 T25839 compound rabbit,mAb
R17654 T25839 T25832 conj mAb,pAb
R17655 T25840 T25839 punct ",",mAb
R17656 T25841 T25839 conj α-tubulin,mAb
R17657 T25842 T25841 punct (,α-tubulin
R17658 T25843 T25844 compound clone,DM1A
R17659 T25844 T25841 appos DM1A,α-tubulin
R17660 T25845 T25841 punct ),α-tubulin
R17661 T25846 T25847 compound mouse,mAb
R17662 T25847 T25839 conj mAb,mAb
R17663 T25848 T25847 punct ",",mAb
R17664 T25849 T25847 conj phospho-FoxO1,mAb
R17665 T25850 T25849 punct (,phospho-FoxO1
R17666 T25851 T25849 appos Thr24,phospho-FoxO1
R17667 T25852 T25853 dep ),/
R17668 T25853 T25854 compound /,FoxO3a
R17669 T25854 T25849 conj FoxO3a,phospho-FoxO1
R17670 T25855 T25854 punct (,FoxO3a
R17671 T25856 T25854 appos Thr32,FoxO3a
R17672 T25857 T25854 punct ),FoxO3a
R17673 T25858 T25859 compound rabbit,pAb
R17674 T25859 T25849 conj pAb,phospho-FoxO1
R17675 T25860 T25859 punct ",",pAb
R17676 T25861 T25859 conj FoxO1,pAb
R17677 T25862 T25861 punct (,FoxO1
R17678 T25863 T25861 appos L27,FoxO1
R17679 T25864 T25861 punct ),FoxO1
R17680 T25865 T25866 compound rabbit,pAb
R17681 T25866 T25859 conj pAb,pAb
R17682 T25867 T25866 punct ",",pAb
R17683 T25868 T25866 conj phospho-FoxO4,pAb
R17684 T25869 T25870 punct (,Ser193
R17685 T25870 T25868 appos Ser193,phospho-FoxO4
R17686 T25871 T25868 punct ),phospho-FoxO4
R17687 T25872 T25873 compound rabbit,pAb
R17688 T25873 T25866 conj pAb,pAb
R17689 T25874 T25873 punct ",",pAb
R17690 T25875 T25876 compound FoxO4,rabbit
R17691 T25876 T25877 compound rabbit,pAb
R17692 T25877 T25873 conj pAb,pAb
R17693 T25878 T25877 punct ",",pAb
R17694 T25879 T25877 conj phospho-MDM2,pAb
R17695 T25880 T25881 punct (,Ser166
R17696 T25881 T25879 appos Ser166,phospho-MDM2
R17697 T25882 T25879 punct ),phospho-MDM2
R17698 T25883 T25884 compound rabbit,pAb
R17699 T25884 T25877 conj pAb,pAb
R17700 T25885 T25884 punct ",",pAb
R17701 T25886 T25888 compound phospho-GSK-3α,β
R17702 T25887 T25888 compound /,β
R17703 T25888 T25884 conj β,pAb
R17704 T25889 T25890 punct (,Ser21/9
R17705 T25890 T25888 appos Ser21/9,β
R17706 T25891 T25888 punct ),β
R17707 T25892 T25893 compound rabbit,pAb
R17708 T25893 T25888 conj pAb,β
R17709 T25894 T25893 punct ",",pAb
R17710 T25895 T25897 compound phospho-p70,Kinase
R17711 T25896 T25897 compound S6,Kinase
R17712 T25897 T25893 conj Kinase,pAb
R17713 T25898 T25899 punct (,Thr389
R17714 T25899 T25897 appos Thr389,Kinase
R17715 T25900 T25897 punct ),Kinase
R17716 T25901 T25903 punct (,108D2
R17717 T25902 T25903 compound clone,108D2
R17718 T25903 T25897 appos 108D2,Kinase
R17719 T25904 T25903 punct ),108D2
R17720 T25905 T25906 compound rabbit,mAb
R17721 T25906 T25897 conj mAb,Kinase
R17722 T25907 T25906 punct ",",mAb
R17723 T25908 T25910 compound phospho-S6,Protein
R17724 T25909 T25910 compound Ribosomal,Protein
R17725 T25910 T25906 conj Protein,mAb
R17726 T25911 T25912 punct (,Ser235/236
R17727 T25912 T25910 appos Ser235/236,Protein
R17728 T25913 T25910 punct ),Protein
R17729 T25914 T25922 punct (,mAb
R17730 T25915 T25918 nmod clone,E
R17731 T25916 T25918 nmod D57,E
R17732 T25917 T25918 nummod .2.2,E
R17733 T25918 T25921 nmod E,rabbit
R17734 T25919 T25918 punct ),E
R17735 T25920 T25921 compound XP,rabbit
R17736 T25921 T25922 compound rabbit,mAb
R17737 T25922 T25910 conj mAb,Protein
R17738 T25923 T25922 punct ",",mAb
R17739 T25924 T25926 compound S6,Protein
R17740 T25925 T25926 compound Ribosomal,Protein
R17741 T25926 T25922 appos Protein,mAb
R17742 T25927 T25928 punct (,clone
R17743 T25928 T25929 compound clone,54D2
R17744 T25929 T25926 appos 54D2,Protein
R17745 T25930 T25926 punct ),Protein
R17746 T25931 T25932 compound mouse,mAb
R17747 T25932 T25922 conj mAb,mAb
R17748 T25933 T25932 punct ",",mAb
R17749 T25934 T25932 conj phospho-4E-BP1,mAb
R17750 T25935 T25936 punct (,Thr37/46
R17751 T25936 T25934 appos Thr37/46,phospho-4E-BP1
R17752 T25937 T25934 punct ),phospho-4E-BP1
R17753 T25938 T25939 compound rabbit,pAb
R17754 T25939 T25932 conj pAb,mAb
R17755 T25940 T25939 punct ",",pAb
R17756 T25941 T25944 compound mTOR,7C10
R17757 T25942 T25943 punct (,clone
R17758 T25943 T25944 compound clone,7C10
R17759 T25944 T25939 appos 7C10,pAb
R17760 T25945 T25939 punct ),pAb
R17761 T25946 T25947 compound rabbit,mAb
R17762 T25947 T25939 conj mAb,pAb
R17763 T25948 T25947 punct ",",mAb
R17764 T25949 T25951 compound PDK1,pAb
R17765 T25950 T25951 compound rabbit,pAb
R17766 T25951 T25947 conj pAb,mAb
R17767 T25952 T25955 punct (,Signaling
R17768 T25953 T25955 det all,Signaling
R17769 T25954 T25955 compound Cell,Signaling
R17770 T25955 T25951 parataxis Signaling,pAb
R17771 T25956 T25955 punct ),Signaling
R17772 T25957 T25951 punct ",",pAb
R17773 T25958 T25960 compound MDM2,pAb
R17774 T25959 T25960 compound rabbit,pAb
R17775 T25960 T25951 conj pAb,pAb
R17776 T25961 T25962 punct (,Bioworlde
R17777 T25962 T25960 appos Bioworlde,pAb
R17778 T25963 T25960 punct ),pAb
R17779 T25964 T25752 punct .,used
R17827 T26093 T26095 compound Primers,TNFα
R17828 T26094 T26095 compound Mouse,TNFα
R17829 T26095 T26095 ROOT TNFα,TNFα
R17830 T26096 T26102 punct :,′
R17831 T26097 T26102 advmod forward,′
R17832 T26098 T26102 nummod 5,′
R17833 T26099 T26101 punct ′,CCCTCACACTCAGATCATCTTCT-3
R17834 T26100 T26101 punct -,CCCTCACACTCAGATCATCTTCT-3
R17835 T26101 T26102 compound CCCTCACACTCAGATCATCTTCT-3,′
R17836 T26102 T26095 appos ′,TNFα
R17837 T26103 T26102 punct ",",′
R17838 T26104 T26120 ccomp reverse,reverse
R17839 T26105 T26109 nummod 5,′
R17840 T26106 T26108 punct ′,GCTACGACGTGGGCTACAG-3
R17841 T26107 T26108 punct -,GCTACGACGTGGGCTACAG-3
R17842 T26108 T26109 compound GCTACGACGTGGGCTACAG-3,′
R17843 T26109 T26104 dobj ′,reverse
R17844 T26110 T26120 punct ;,reverse
R17845 T26111 T26112 compound mouse,18S
R17846 T26112 T26120 nsubj 18S,reverse
R17847 T26113 T26112 punct :,18S
R17848 T26114 T26118 advmod forward,ATAACAGGTCTGTGATGCCCTTAG-3
R17849 T26115 T26117 meta 5,′
R17850 T26116 T26117 punct -,′
R17851 T26117 T26118 compound ′,ATAACAGGTCTGTGATGCCCTTAG-3
R17852 T26118 T26112 appos ATAACAGGTCTGTGATGCCCTTAG-3,18S
R17853 T26119 T26118 punct ",",ATAACAGGTCTGTGATGCCCTTAG-3
R17854 T26120 T26160 ccomp reverse,reverse
R17855 T26121 T26125 nummod 5,′
R17856 T26122 T26124 punct ′,CTAAACCATCCAATCGGTAGTAGC-3
R17857 T26123 T26124 punct -,CTAAACCATCCAATCGGTAGTAGC-3
R17858 T26124 T26125 compound CTAAACCATCCAATCGGTAGTAGC-3,′
R17859 T26125 T26120 dobj ′,reverse
R17860 T26126 T26160 punct ;,reverse
R17861 T26127 T26128 amod human,TNFα
R17862 T26128 T26160 nsubj TNFα,reverse
R17863 T26129 T26135 punct :,′
R17864 T26130 T26135 advmod forward,′
R17865 T26131 T26134 nummod 5,ATGAGCACTGAAAGCATGATCC-3
R17866 T26132 T26134 punct ′,ATGAGCACTGAAAGCATGATCC-3
R17867 T26133 T26134 punct -,ATGAGCACTGAAAGCATGATCC-3
R17868 T26134 T26135 compound ATGAGCACTGAAAGCATGATCC-3,′
R17869 T26135 T26128 appos ′,TNFα
R17872 T26138 T26128 conj TNFα,TNFα
R17882 T26148 T26160 nsubj 18S,reverse
R17883 T26149 T26148 punct :,18S
R17884 T26150 T26158 advmod forward,18S
R17885 T26151 T26158 nummod 5,18S
R17886 T26152 T26154 punct ′,CAGCCACCCGAGATTGAGCA
R17887 T26153 T26154 punct -,CAGCCACCCGAGATTGAGCA
R17888 T26154 T26158 nmod CAGCCACCCGAGATTGAGCA,18S
R17889 T26155 T26154 nummod -3,CAGCCACCCGAGATTGAGCA
R17890 T26156 T26158 punct ",",18S
R18023 T26334 T26336 compound Cell,L929
R18024 T26335 T26336 compound Lines,L929
R18025 T26336 T26342 nsubjpass L929,obtained
R18026 T26337 T26336 cc and,L929
R18027 T26338 T26339 compound FADD-deficient,Jurkat
R18028 T26339 T26336 conj Jurkat,L929
R18029 T26340 T26342 nsubjpass cells,obtained
R18030 T26341 T26342 auxpass were,obtained
R18031 T26342 T26342 ROOT obtained,obtained
R18032 T26343 T26342 prep from,obtained
R18033 T26344 T26343 pobj ATCC,from
R18034 T26345 T26342 punct .,obtained
R18035 T26346 T26347 compound Lung,fibroblasts
R18036 T26347 T26348 nsubj fibroblasts,were
R18037 T26348 T26348 ROOT were,were
R18038 T26349 T26351 det a,gift
R18039 T26350 T26351 amod generous,gift
R18040 T26351 T26348 attr gift,were
R18041 T26352 T26351 prep of,gift
R18042 T26353 T26355 compound Dr.,Tsichlis
R18043 T26354 T26355 compound Philip,Tsichlis
R18044 T26355 T26352 pobj Tsichlis,of
R18045 T26356 T26355 punct (,Tsichlis
R18046 T26357 T26358 compound Tufts,University
R18047 T26358 T26355 appos University,Tsichlis
R18048 T26359 T26355 punct ),Tsichlis
R18049 T26360 T26362 nmod [,]
R18050 T26361 T26362 nummod 53,]
R18051 T26362 T26351 appos ],gift
R18052 T26363 T26348 punct .,were
R18053 T26364 T26365 nummod J774A,.1
R18054 T26365 T26369 nummod .1,cells
R18055 T26366 T26367 punct (,ATCC
R18056 T26367 T26367 ROOT ATCC,ATCC
R18057 T26368 T26367 punct ),ATCC
R18058 T26369 T26377 nsubj cells,were
R18059 T26370 T26369 cc and,cells
R18060 T26371 T26376 nmod RAW264,cells
R18061 T26372 T26371 nummod .7,RAW264
R18062 T26373 T26374 punct (,ATCC
R18063 T26374 T26371 appos ATCC,RAW264
R18064 T26375 T26371 punct ),RAW264
R18065 T26376 T26369 conj cells,cells
R18066 T26377 T26377 ROOT were,were
R18067 T26378 T26379 amod generous,gifts
R18068 T26379 T26377 attr gifts,were
R18069 T26380 T26379 prep of,gifts
R18070 T26381 T26382 compound Junying,Yuan
R18071 T26382 T26380 pobj Yuan,of
R18072 T26383 T26385 punct (,University
R18073 T26384 T26385 compound Harvard,University
R18074 T26385 T26382 appos University,Yuan
R18075 T26386 T26385 punct ),University
R18076 T26387 T26382 cc and,Yuan
R18077 T26388 T26389 compound Alexander,Poltorak
R18078 T26389 T26382 conj Poltorak,Yuan
R18079 T26390 T26392 punct (,University
R18080 T26391 T26392 compound Tufts,University
R18081 T26392 T26389 appos University,Poltorak
R18082 T26393 T26389 punct ),Poltorak
R18083 T26394 T26379 punct ",",gifts
R18084 T26395 T26377 advmod respectively,were
R18085 T26396 T26377 punct .,were
R18086 T26397 T26399 nsubjpass Cells,maintained
R18087 T26398 T26399 auxpass were,maintained
R18088 T26399 T26399 ROOT maintained,maintained
R18089 T26400 T26399 prep in,maintained
R18090 T26401 T26400 pobj DMEM,in
R18091 T26402 T26399 conj supplemented,maintained
R18092 T26403 T26402 prep with,supplemented
R18093 T26404 T26405 nummod 10,%
R18094 T26405 T26408 nmod %,serum
R18095 T26406 T26408 amod fetal,serum
R18096 T26407 T26408 amod bovine,serum
R18097 T26408 T26403 pobj serum,with
R18098 T26409 T26410 punct (,FBS
R18099 T26410 T26408 appos FBS,serum
R18100 T26411 T26410 punct ),FBS
R18101 T26412 T26408 cc and,serum
R18102 T26413 T26414 nummod 1,%
R18103 T26414 T26416 nmod %,mixture
R18104 T26415 T26416 amod antibiotic-antimycotic,mixture
R18105 T26416 T26408 conj mixture,serum
R18106 T26417 T26418 punct (,Invitrogen
R18107 T26418 T26416 appos Invitrogen,mixture
R18108 T26419 T26408 punct ),serum
R18109 T26420 T26399 punct .,maintained
R18110 T26421 T26425 det The,media
R18111 T26422 T26425 compound mouse,media
R18112 T26423 T26425 compound lung,media
R18113 T26424 T26425 compound fibroblast,media
R18114 T26425 T26428 nsubjpass media,supplemented
R18115 T26426 T26428 auxpass was,supplemented
R18116 T26427 T26428 advmod additionally,supplemented
R18117 T26428 T26428 ROOT supplemented,supplemented
R18118 T26429 T26428 prep with,supplemented
R18119 T26430 T26429 pobj L-glutamine,with
R18120 T26431 T26430 punct ",",L-glutamine
R18121 T26432 T26434 amod non-essential,acids
R18122 T26433 T26434 amod amino,acids
R18123 T26434 T26430 conj acids,L-glutamine
R18124 T26435 T26434 punct ",",acids
R18125 T26436 T26434 cc and,acids
R18126 T26437 T26438 compound sodium,pyruvate
R18127 T26438 T26434 conj pyruvate,acids
R18128 T26439 T26428 punct .,supplemented
R18129 T26440 T26441 compound Jurkat,cells
R18130 T26441 T26443 nsubjpass cells,maintained
R18131 T26442 T26443 auxpass were,maintained
R18132 T26443 T26443 ROOT maintained,maintained
R18133 T26444 T26443 prep in,maintained
R18134 T26445 T26444 pobj RPMI1640,in
R18135 T26446 T26443 punct ",",maintained
R18136 T26447 T26443 conj supplemented,maintained
R18137 T26448 T26447 prep with,supplemented
R18138 T26449 T26450 nummod 10,%
R18139 T26450 T26451 compound %,FetalPlex
R18140 T26451 T26448 pobj FetalPlex,with
R18141 T26452 T26451 punct (,FetalPlex
R18142 T26453 T26451 appos Gemini,FetalPlex
R18143 T26454 T26451 punct ),FetalPlex
R18144 T26455 T26447 cc and,supplemented
R18145 T26456 T26457 nummod 1,%
R18146 T26457 T26458 compound %,antibiotic-antimycotic
R18147 T26458 T26447 conj antibiotic-antimycotic,supplemented
R18148 T26459 T26443 punct .,maintained
R18252 T26586 T26588 nsubjpass Cells,seeded
R18253 T26587 T26588 auxpass were,seeded
R18254 T26588 T26588 ROOT seeded,seeded
R18255 T26589 T26588 prep into,seeded
R18256 T26590 T26592 amod white,bottom
R18257 T26591 T26592 amod clear,bottom
R18258 T26592 T26589 pobj bottom,into
R18259 T26593 T26592 nummod 96,bottom
R18260 T26594 T26595 intj well,plates
R18261 T26595 T26588 conj plates,seeded
R18262 T26596 T26595 prep at,plates
R18263 T26597 T26598 det the,density
R18264 T26598 T26596 pobj density,at
R18265 T26599 T26598 prep of,density
R18266 T26600 T26603 nummod 1,cells/well
R18267 T26601 T26603 nummod ×,cells/well
R18268 T26602 T26603 nummod 104,cells/well
R18269 T26603 T26599 pobj cells/well,of
R18270 T26604 T26595 cc and,plates
R18271 T26605 T26595 conj treated,plates
R18272 T26606 T26607 mark as,described
R18273 T26607 T26595 advcl described,plates
R18274 T26608 T26607 prep for,described
R18275 T26609 T26611 amod western,experiments
R18276 T26610 T26611 compound blot,experiments
R18277 T26611 T26608 pobj experiments,for
R18278 T26612 T26588 punct .,seeded
R18279 T26613 T26614 compound Cell,viability
R18280 T26614 T26616 nsubjpass viability,determined
R18281 T26615 T26616 auxpass was,determined
R18282 T26616 T26616 ROOT determined,determined
R18283 T26617 T26616 advcl using,determined
R18284 T26618 T26621 compound CellTiter-Glo,Assay
R18285 T26619 T26621 compound Cell,Assay
R18286 T26620 T26621 compound Viability,Assay
R18287 T26621 T26617 dobj Assay,using
R18288 T26622 T26621 punct (,Assay
R18289 T26623 T26621 appos Promega,Assay
R18290 T26624 T26621 punct ),Assay
R18291 T26625 T26616 punct .,determined
R18293 T26627 T26628 auxpass were,performed
R18294 T26628 T26628 ROOT performed,performed
R18295 T26629 T26628 prep in,performed
R18296 T26630 T26629 pobj duplicate,in
R18297 T26631 T26630 cc or,duplicate
R18298 T26632 T26630 conj triplicate,duplicate
R18299 T26633 T26628 punct .,performed
R18300 T26634 T26641 nsubjpass Viability,set
R18301 T26635 T26634 prep of,Viability
R18302 T26636 T26637 det the,control
R18303 T26637 T26639 amod control,cells
R18304 T26638 T26639 amod untreated,cells
R18305 T26639 T26635 pobj cells,of
R18306 T26640 T26641 auxpass was,set
R18307 T26641 T26641 ROOT set,set
R18308 T26642 T26641 prep as,set
R18309 T26643 T26644 nummod 100,%
R18310 T26644 T26642 pobj %,as
R18311 T26645 T26641 punct .,set
R18312 T26646 T26647 amod Relative,viability
R18313 T26647 T26651 nsubj viability,induced
R18314 T26648 T26647 prep of,viability
R18315 T26649 T26648 pobj cells,of
R18316 T26650 T26651 punct ",",induced
R18317 T26651 T26651 ROOT induced,induced
R18318 T26652 T26653 aux to,undergo
R18319 T26653 T26651 xcomp undergo,induced
R18320 T26654 T26653 dobj necroptosis,undergo
R18321 T26655 T26651 cc and,induced
R18322 T26656 T26651 conj treated,induced
R18323 T26657 T26656 prep with,treated
R18324 T26658 T26659 det the,compound
R18325 T26659 T26657 pobj compound,with
R18326 T26660 T26659 amod relative,compound
R18327 T26661 T26660 prep to,relative
R18328 T26662 T26663 det the,control
R18329 T26663 T26661 pobj control,to
R18330 T26664 T26665 amod compound-treated,cells
R18331 T26665 T26659 appos cells,compound
R18332 T26666 T26668 punct ",",determined
R18333 T26667 T26668 auxpass was,determined
R18334 T26668 T26651 conj determined,induced
R18335 T26669 T26668 cc and,determined
R18336 T26670 T26668 conj plotted,determined
R18337 T26671 T26672 aux to,exclude
R18338 T26672 T26670 xcomp exclude,plotted
R18339 T26673 T26675 det the,effects
R18340 T26674 T26675 amod possible,effects
R18341 T26675 T26672 dobj effects,exclude
R18342 T26676 T26675 prep of,effects
R18343 T26677 T26678 amod non-specific,toxicity
R18344 T26678 T26676 pobj toxicity,of
R18345 T26679 T26678 prep of,toxicity
R18346 T26680 T26682 det the,molecules
R18347 T26681 T26682 amod small,molecules
R18348 T26682 T26679 pobj molecules,of
R18349 T26683 T26651 punct .,induced
R18486 T26929 T26931 compound siRNA,siRNAs
R18487 T26930 T26931 compound Knockdown,siRNAs
R18488 T26931 T26933 nsubjpass siRNAs,purchased
R18489 T26932 T26933 auxpass were,purchased
R18490 T26933 T26933 ROOT purchased,purchased
R18491 T26934 T26933 prep from,purchased
R18492 T26935 T26934 pobj Dharmacon,from
R18493 T26936 T26933 punct .,purchased
R18494 T26937 T26940 nmod Mouse,protein
R18495 T26938 T26940 amod ribosomal,protein
R18496 T26939 T26940 compound S6,protein
R18497 T26940 T26940 ROOT protein,protein
R18498 T26941 T26942 punct (,L-040893-00
R18499 T26942 T26940 appos L-040893-00,protein
R18500 T26943 T26942 cc and,L-040893-00
R18501 T26944 T26942 conj L-045791-00,L-040893-00
R18502 T26945 T26944 punct ),L-045791-00
R18503 T26946 T26942 punct ",",L-040893-00
R18504 T26947 T26948 compound mouse,Akt1
R18505 T26948 T26942 appos Akt1,L-040893-00
R18506 T26949 T26950 punct (,L-040709-00
R18507 T26950 T26948 appos L-040709-00,Akt1
R18508 T26951 T26950 punct ),L-040709-00
R18509 T26952 T26948 punct ",",Akt1
R18510 T26953 T26954 compound mouse,Akt2
R18511 T26954 T26948 appos Akt2,Akt1
R18512 T26955 T26956 punct (,L-040782-00
R18513 T26956 T26954 appos L-040782-00,Akt2
R18514 T26957 T26956 punct ),L-040782-00
R18515 T26958 T26954 punct ",",Akt2
R18516 T26959 T26960 compound mouse,Akt3
R18517 T26960 T26954 conj Akt3,Akt2
R18518 T26961 T26962 punct (,L-040891-00
R18519 T26962 T26960 appos L-040891-00,Akt3
R18520 T26963 T26962 punct ),L-040891-00
R18521 T26964 T26960 punct ",",Akt3
R18522 T26965 T26966 compound mouse,mTOR
R18523 T26966 T26960 conj mTOR,Akt3
R18524 T26967 T26968 punct (,L-065427-00
R18525 T26968 T26966 appos L-065427-00,mTOR
R18526 T26969 T26966 punct ),mTOR
R18527 T26970 T26966 punct ",",mTOR
R18528 T26971 T26972 compound mouse,PDK1
R18529 T26972 T26966 conj PDK1,mTOR
R18530 T26973 T26974 punct (,L-040658-00
R18531 T26974 T26972 appos L-040658-00,PDK1
R18532 T26975 T26972 punct ),PDK1
R18533 T26976 T26972 punct ",",PDK1
R18534 T26977 T26978 amod non-coding,control
R18535 T26978 T26972 appos control,PDK1
R18536 T26979 T26980 punct (,D-001810-10-05
R18537 T26980 T26972 appos D-001810-10-05,PDK1
R18538 T26981 T26980 punct ),D-001810-10-05
R18539 T26982 T26972 punct ",",PDK1
R18540 T26983 T26984 compound mouse,Mapk8
R18541 T26984 T26972 conj Mapk8,PDK1
R18570 T27013 T27014 poss manufacturer,recommendations
R18571 T27014 T27012 pobj recommendations,to
R18572 T27015 T27003 punct .,transfected
R18573 T27016 T27022 prep After,treated
R18574 T27017 T27018 nummod 72,hr
R18575 T27018 T27016 pobj hr,After
R18576 T27019 T27022 punct ",",treated
R18577 T27020 T27022 nsubjpass cells,treated
R18578 T27021 T27022 auxpass were,treated
R18579 T27022 T27022 ROOT treated,treated
R18580 T27023 T27022 prep with,treated
R18581 T27024 T27023 pobj zVAD.fmk,with
R18582 T27025 T27024 cc or,zVAD.fmk
R18583 T27026 T27024 conj TNFα,zVAD.fmk
R18584 T27027 T27022 prep for,treated
R18585 T27028 T27029 nummod 9,hr
R18586 T27029 T27027 pobj hr,for
R18587 T27030 T27029 punct (,hr
R18588 T27031 T27029 appos RNA,hr
R18589 T27032 T27031 cc or,RNA
R18590 T27033 T27034 compound Western,blot
R18591 T27034 T27031 conj blot,RNA
R18592 T27035 T27034 punct ),blot
R18593 T27036 T27034 cc or,blot
R18594 T27037 T27038 nummod 24,hr
R18595 T27038 T27034 conj hr,blot
R18596 T27039 T27041 punct (,viability
R18597 T27040 T27041 compound cell,viability
R18598 T27041 T27038 appos viability,hr
R18599 T27042 T27038 punct ),hr
R18600 T27043 T27022 punct .,treated
R18819 T27367 T27368 amod Western,Blot
R18820 T27368 T27386 nsubjpass Blot,seeded
R18821 T27369 T27386 prep For,seeded
R18822 T27370 T27371 amod Western,blot
R18823 T27371 T27369 pobj blot,For
R18824 T27372 T27386 punct ",",seeded
R18825 T27373 T27377 nummod 4,cells
R18826 T27374 T27377 nummod ×,cells
R18827 T27375 T27377 nummod 105,cells
R18828 T27376 T27377 amod adherent,cells
R18829 T27377 T27386 nsubjpass cells,seeded
R18830 T27378 T27377 punct (,cells
R18831 T27379 T27383 nummod 1,cells
R18832 T27380 T27383 nummod ×,cells
R18833 T27381 T27383 nummod 106,cells
R18834 T27382 T27383 compound Jurkat,cells
R18835 T27383 T27377 appos cells,cells
R18836 T27384 T27377 punct ),cells
R18837 T27385 T27386 auxpass were,seeded
R18838 T27386 T27386 ROOT seeded,seeded
R18839 T27387 T27386 prep into,seeded
R18840 T27388 T27390 nummod 35,dishes
R18841 T27389 T27390 nummod mm2,dishes
R18842 T27390 T27387 pobj dishes,into
R18843 T27391 T27386 punct .,seeded
R18844 T27392 T27399 prep After,stimulated
R18845 T27393 T27392 pobj 24,After
R18846 T27394 T27395 nummod 48,hr
R18847 T27395 T27393 appos hr,24
R18848 T27396 T27399 punct ",",stimulated
R18849 T27397 T27399 nsubjpass cells,stimulated
R18850 T27398 T27399 auxpass were,stimulated
R18851 T27399 T27399 ROOT stimulated,stimulated
R18852 T27400 T27399 prep with,stimulated
R18853 T27401 T27403 nummod 30,zVAD.fmk
R18854 T27402 T27403 compound µM,zVAD.fmk
R18855 T27403 T27400 pobj zVAD.fmk,with
R18856 T27404 T27403 cc or,zVAD.fmk
R18857 T27405 T27407 nummod 10,mouse
R18858 T27406 T27407 amod ng/ml,mouse
R18859 T27407 T27408 compound mouse,TNFα
R18860 T27408 T27403 conj TNFα,zVAD.fmk
R18861 T27409 T27399 punct .,stimulated
R18862 T27410 T27420 prep For,starved
R18863 T27411 T27410 pobj treatments,For
R18864 T27412 T27411 prep under,treatments
R18865 T27413 T27415 nmod serum,conditions
R18866 T27414 T27415 amod free,conditions
R18867 T27415 T27412 pobj conditions,under
R18868 T27416 T27420 punct ",",starved
R18869 T27417 T27420 nsubjpass cells,starved
R18870 T27418 T27420 auxpass were,starved
R18871 T27419 T27420 advmod serum,starved
R18872 T27420 T27420 ROOT starved,starved
R18873 T27421 T27420 prep for,starved
R18874 T27422 T27423 nummod 24,hr
R18875 T27423 T27421 pobj hr,for
R18876 T27424 T27420 advmod prior,starved
R18877 T27425 T27424 prep to,prior
R18878 T27426 T27427 det the,addition
R18879 T27427 T27425 pobj addition,to
R18880 T27428 T27427 prep of,addition
R18881 T27429 T27430 compound growth,factors
R18882 T27430 T27428 pobj factors,of
R18883 T27431 T27430 punct ",",factors
R18884 T27432 T27434 nummod 20,zVAD.fmk
R18885 T27433 T27434 compound µM,zVAD.fmk
R18886 T27434 T27430 appos zVAD.fmk,factors
R18887 T27435 T27434 cc or,zVAD.fmk
R18888 T27436 T27439 nummod 10,TNFα
R18889 T27437 T27438 amod ng/ml,mouse
R18890 T27438 T27439 compound mouse,TNFα
R18891 T27439 T27434 conj TNFα,zVAD.fmk
R18892 T27440 T27420 punct .,starved
R18893 T27441 T27443 nsubjpass Cells,harvested
R18894 T27442 T27443 auxpass were,harvested
R18895 T27443 T27443 ROOT harvested,harvested
R18896 T27444 T27443 prep in,harvested
R18897 T27445 T27446 nummod 1,×
R18898 T27446 T27448 nummod ×,buffer
R18899 T27447 T27448 compound RIPA,buffer
R18900 T27448 T27444 pobj buffer,in
R18901 T27449 T27448 punct (,buffer
R18902 T27450 T27451 compound Cell,Signaling
R18903 T27451 T27448 appos Signaling,buffer
R18904 T27452 T27448 punct ),buffer
R18905 T27453 T27443 conj supplemented,harvested
R18906 T27454 T27453 prep with,supplemented
R18907 T27455 T27456 nummod 50,µg
R18908 T27456 T27459 nmod µg,phenylmethanesulfonylfluoride
R18909 T27457 T27459 nmod /,phenylmethanesulfonylfluoride
R18910 T27458 T27459 compound ml,phenylmethanesulfonylfluoride
R18911 T27459 T27454 pobj phenylmethanesulfonylfluoride,with
R18912 T27460 T27443 punct .,harvested
R18913 T27461 T27468 prep After,spun
R18914 T27462 T27463 amod brief,sonication
R18915 T27463 T27468 advcl sonication,spun
R18916 T27464 T27468 punct ",",spun
R18917 T27465 T27466 compound cell,lysates
R18918 T27466 T27468 nsubjpass lysates,spun
R18919 T27467 T27468 auxpass were,spun
R18920 T27468 T27468 ROOT spun,spun
R18921 T27469 T27468 prt down,spun
R18922 T27470 T27468 prep for,spun
R18923 T27471 T27472 nummod 15,min
R18924 T27472 T27470 pobj min,for
R18925 T27473 T27472 prep at,min
R18926 T27474 T27475 nummod "14,000",×
R18927 T27475 T27476 nummod ×,rpm
R18928 T27476 T27473 pobj rpm,at
R18929 T27477 T27468 punct .,spun
R18930 T27478 T27479 compound Protein,concentrations
R18931 T27479 T27481 nsubjpass concentrations,measured
R18932 T27480 T27481 auxpass were,measured
R18933 T27481 T27481 ROOT measured,measured
R18934 T27482 T27481 advcl using,measured
R18935 T27483 T27484 det the,Pierce
R18936 T27484 T27482 dobj Pierce,using
R18937 T27485 T27484 nummod 660,Pierce
R18938 T27486 T27488 compound nm,Reagent
R18939 T27487 T27488 compound Assay,Reagent
R18940 T27488 T27484 conj Reagent,Pierce
R18941 T27489 T27490 punct (,Pierce
R18942 T27490 T27488 appos Pierce,Reagent
R18943 T27491 T27488 punct ),Reagent
R18944 T27492 T27481 punct .,measured
R18945 T27493 T27494 compound Equal,amounts
R18946 T27494 T27498 nsubjpass amounts,boiled
R18947 T27495 T27494 prep of,amounts
R18948 T27496 T27495 pobj proteins,of
R18949 T27497 T27498 auxpass were,boiled
R18950 T27498 T27498 ROOT boiled,boiled
R18951 T27499 T27498 prep for,boiled
R18952 T27500 T27501 nummod 5,min
R18953 T27501 T27499 pobj min,for
R18954 T27502 T27498 prep at,boiled
R18955 T27503 T27504 nummod 95,°
R18956 T27504 T27507 nummod °,blotting
R18957 T27505 T27506 compound C.,Western
R18958 T27506 T27507 compound Western,blotting
R18959 T27507 T27502 pobj blotting,at
R18960 T27508 T27509 auxpass was,performed
R18961 T27509 T27498 conj performed,boiled
R18962 T27510 T27509 prep according,performed
R18963 T27511 T27510 prep to,according
R18964 T27512 T27513 amod standard,protocols
R18965 T27513 T27511 pobj protocols,to
R18966 T27514 T27498 punct .,boiled
R18967 T27515 T27520 advmod Briefly,transferred
R18968 T27516 T27520 punct ",",transferred
R18969 T27517 T27518 compound SDS-PAGE,gels
R18970 T27518 T27520 nsubjpass gels,transferred
R18971 T27519 T27520 auxpass were,transferred
R18972 T27520 T27520 ROOT transferred,transferred
R18973 T27521 T27520 prep to,transferred
R18974 T27522 T27523 compound PVDF,membrane
R18975 T27523 T27521 pobj membrane,to
R18976 T27524 T27523 punct ",",membrane
R18977 T27525 T27520 conj blocked,transferred
R18978 T27526 T27525 prep in,blocked
R18979 T27527 T27528 nummod 3,%
R18980 T27528 T27529 compound %,milk
R18981 T27529 T27526 pobj milk,in
R18983 T27531 T27532 nummod 5,%
R18984 T27532 T27535 compound %,albumin
R18985 T27533 T27534 amod bovine,serum
R18986 T27534 T27535 compound serum,albumin
R18987 T27535 T27529 conj albumin,milk
R18988 T27536 T27537 punct (,BSA
R18989 T27537 T27535 appos BSA,albumin
R18990 T27538 T27525 punct ),blocked
R18991 T27539 T27525 prep in,blocked
R18992 T27540 T27541 compound TBST,buffer
R18993 T27541 T27539 pobj buffer,in
R18994 T27542 T27525 prep for,blocked
R18995 T27543 T27544 nummod 30,min
R18996 T27544 T27542 pobj min,for
R18997 T27545 T27544 prep at,min
R18998 T27546 T27547 compound room,temperature
R18999 T27547 T27545 pobj temperature,at
R19000 T27548 T27520 punct .,transferred
R19001 T27549 T27550 amod Primary,antibodies
R19002 T27550 T27552 nsubjpass antibodies,incubated
R19003 T27551 T27552 auxpass were,incubated
R19004 T27552 T27552 ROOT incubated,incubated
R19005 T27553 T27552 prep in,incubated
R19006 T27554 T27555 nummod 5,%
R19007 T27555 T27556 compound %,BSA/TBST
R19008 T27556 T27557 compound BSA/TBST,overnight
R19009 T27557 T27553 pobj overnight,in
R19010 T27558 T27552 prep at,incubated
R19011 T27559 T27560 nummod 4,°
R19012 T27560 T27563 nummod °,antibodies
R19013 T27561 T27563 nmod C.,antibodies
R19014 T27562 T27563 amod Secondary,antibodies
R19015 T27563 T27558 pobj antibodies,at
R19016 T27564 T27565 auxpass were,incubated
R19017 T27565 T27552 conj incubated,incubated
R19018 T27566 T27565 prep in,incubated
R19019 T27567 T27566 pobj TBST,in
R19020 T27568 T27565 prep for,incubated
R19021 T27569 T27570 nummod 30,min
R19022 T27570 T27568 pobj min,for
R19023 T27571 T27570 prep at,min
R19024 T27572 T27573 compound room,temperature
R19025 T27573 T27571 pobj temperature,at
R19026 T27574 T27552 punct .,incubated
R19027 T27575 T27575 ROOT Luminata,Luminata
R19028 T27576 T27577 punct (,Millipore
R19029 T27577 T27575 appos Millipore,Luminata
R19030 T27578 T27577 punct ),Millipore
R19031 T27579 T27580 compound ECL,reagents
R19032 T27580 T27582 nsubjpass reagents,used
R19033 T27581 T27582 auxpass were,used
R19034 T27582 T27582 ROOT used,used
R19035 T27583 T27584 aux to,develop
R19036 T27584 T27582 xcomp develop,used
R19037 T27585 T27586 det the,signals
R19038 T27586 T27584 dobj signals,develop
R19039 T27587 T27582 punct .,used
R19040 T27588 T27594 prep In,stripped
R19041 T27589 T27590 det some,cases
R19042 T27590 T27588 pobj cases,In
R19043 T27591 T27594 punct ",",stripped
R19044 T27592 T27594 nsubjpass membranes,stripped
R19045 T27593 T27594 auxpass were,stripped
R19046 T27594 T27594 ROOT stripped,stripped
R19047 T27595 T27594 advcl using,stripped
R19048 T27596 T27595 dobj OneMinute,using
R19049 T27597 T27595 conj stripping,using
R19050 T27598 T27597 dobj buffer,stripping
R19051 T27599 T27601 punct (,Biosciences
R19052 T27600 T27601 compound GM,Biosciences
R19053 T27601 T27598 appos Biosciences,buffer
R19054 T27602 T27601 punct ),Biosciences
R19055 T27603 T27597 cc and,stripping
R19056 T27604 T27597 conj reprobed,stripping
R19057 T27605 T27604 prep with,reprobed
R19058 T27606 T27607 amod new,antibodies
R19059 T27607 T27605 pobj antibodies,with
R19060 T27608 T27594 punct .,stripped
R19129 T27694 T27695 compound qRT-PCR,Cells
R19130 T27695 T27697 nsubjpass Cells,treated
R19131 T27696 T27697 auxpass were,treated
R19132 T27697 T27697 ROOT treated,treated
R19133 T27698 T27699 mark as,described
R19134 T27699 T27697 advcl described,treated
R19135 T27700 T27699 prep for,described
R19136 T27701 T27702 amod Western,blots
R19137 T27702 T27700 pobj blots,for
R19138 T27703 T27697 punct .,treated
R19139 T27704 T27705 amod Total,RNA
R19140 T27705 T27707 nsubjpass RNA,isolated
R19141 T27706 T27707 auxpass was,isolated
R19142 T27707 T27707 ROOT isolated,isolated
R19143 T27708 T27707 advcl using,isolated
R19144 T27709 T27711 compound ZR,kit
R19145 T27710 T27711 compound Miniprep,kit
R19146 T27711 T27708 dobj kit,using
R19147 T27712 T27711 punct (,kit
R19148 T27713 T27714 compound Zymo,Research
R19149 T27714 T27711 appos Research,kit
R19150 T27715 T27711 punct ),kit
R19151 T27716 T27717 punct .,1
R19152 T27717 T27718 nummod 1,µg
R19153 T27718 T27722 nsubjpass µg,converted
R19154 T27719 T27718 prep of,µg
R19155 T27720 T27719 pobj RNA,of
R19156 T27721 T27722 auxpass was,converted
R19157 T27722 T27707 conj converted,isolated
R19158 T27723 T27722 prep to,converted
R19159 T27724 T27723 pobj cDNA,to
R19160 T27725 T27722 advcl using,converted
R19161 T27726 T27727 amod random,primers
R19162 T27727 T27725 dobj primers,using
R19163 T27728 T27727 punct (,primers
R19164 T27729 T27730 compound M-MuLV,cDNA
R19165 T27730 T27731 compound cDNA,kit
R19166 T27731 T27727 appos kit,primers
R19167 T27732 T27731 punct ",",kit
R19168 T27733 T27734 compound New,England
R19169 T27734 T27735 compound England,Biolabs
R19170 T27735 T27731 appos Biolabs,kit
R19171 T27736 T27727 punct ),primers
R19172 T27737 T27722 punct .,converted
R19173 T27738 T27739 nummod 1,µL
R19174 T27739 T27743 nsubjpass µL,used
R19175 T27740 T27739 prep of,µL
R19176 T27741 T27740 pobj cDNA,of
R19177 T27742 T27743 auxpass was,used
R19178 T27743 T27743 ROOT used,used
R19179 T27744 T27743 prep with,used
R19180 T27745 T27746 nummod 500,pM
R19181 T27746 T27747 compound pM,primers
R19182 T27747 T27744 pobj primers,with
R19183 T27748 T27747 prep in,primers
R19184 T27749 T27750 compound qPCR,reactions
R19185 T27750 T27748 pobj reactions,in
R19186 T27751 T27743 punct .,used
R19187 T27752 T27754 nsubj Reactions,performs
R19188 T27753 T27754 advmod were,performs
R19189 T27754 T27754 ROOT performs,performs
R19190 T27755 T27754 advcl using,performs
R19191 T27756 T27760 nmod SYBRGreen,mix
R19192 T27757 T27756 nummod 2,SYBRGreen
R19193 T27758 T27760 nummod ×,mix
R19194 T27759 T27760 compound Master,mix
R19195 T27760 T27755 dobj mix,using
R19196 T27761 T27760 punct (,mix
R19197 T27762 T27760 appos SABiosciences,mix
R19198 T27763 T27760 punct ),mix
R19199 T27764 T27755 prep in,using
R19200 T27765 T27766 det a,LightCycler480
R19201 T27766 T27764 pobj LightCycler480,in
R19202 T27767 T27766 punct (,LightCycler480
R19203 T27768 T27766 appos Roche,LightCycler480
R19204 T27769 T27766 punct ),LightCycler480
R19205 T27770 T27754 punct .,performs
R19298 T27890 T27885 appos Invitrogen,retroviruses
R19299 T27891 T27883 punct ),generate
R19300 T27892 T27893 auxpass were,transfected
R19301 T27893 T27883 dep transfected,generate
R19302 T27894 T27893 prep with,transfected
R19303 T27895 T27896 nummod 2,µg
R19304 T27896 T27894 pobj µg,with
R19305 T27897 T27896 prep of,µg
R19306 T27898 T27899 amod viral,DNA
R19307 T27899 T27897 pobj DNA,of
R19308 T27900 T27896 cc and,µg
R19309 T27901 T27902 nummod 1,µg
R19310 T27902 T27896 conj µg,µg
R19311 T27903 T27902 prep of,µg
R19312 T27904 T27903 pobj gal/pol,of
R19313 T27905 T27904 cc and,gal/pol
R19314 T27906 T27908 compound VSV-G,plasmids
R19315 T27907 T27908 compound accessory,plasmids
R19316 T27908 T27902 conj plasmids,µg
R19317 T27909 T27908 prep in,plasmids
R19318 T27910 T27909 pobj 6,in
R19319 T27911 T27912 intj well,plates
R19320 T27912 T27893 conj plates,transfected
R19321 T27913 T27912 advcl using,plates
R19322 T27914 T27916 nmod GenJet,reagent
R19323 T27915 T27916 compound transfection,reagent
R19324 T27916 T27913 dobj reagent,using
R19325 T27917 T27919 punct (,Labs
R19326 T27918 T27919 compound Signagen,Labs
R19327 T27919 T27916 appos Labs,reagent
R19328 T27920 T27919 punct ),Labs
R19329 T27921 T27883 punct .,generate
R19330 T27922 T27923 amod Virus-containing,media
R19331 T27923 T27925 nsubjpass media,collected
R19332 T27924 T27925 auxpass was,collected
R19333 T27925 T27925 ROOT collected,collected
R19334 T27926 T27928 npadvmod 72,later
R19335 T27927 T27928 npadvmod hr,later
R19336 T27928 T27925 advmod later,collected
R19337 T27929 T27930 punct ",",filtered
R19338 T27930 T27925 advcl filtered,collected
R19339 T27931 T27930 prep through,filtered
R19340 T27932 T27934 nummod 0.45,filter
R19341 T27933 T27934 compound µm,filter
R19342 T27934 T27931 pobj filter,through
R19343 T27935 T27930 cc and,filtered
R19346 T27938 T27939 nummod L929,cells
R19347 T27939 T27937 pobj cells,to
R19348 T27940 T27936 prep with,applied
R19349 T27941 T27942 nummod 8,µg
R19350 T27942 T27945 amod µg,polybrene
R19351 T27943 T27945 nmod /,polybrene
R19352 T27944 T27945 compound ml,polybrene
R19353 T27945 T27940 pobj polybrene,with
R19354 T27946 T27925 punct .,collected
R19355 T27947 T27949 nsubjpass Cells,selected
R19356 T27948 T27949 auxpass were,selected
R19357 T27949 T27949 ROOT selected,selected
R19358 T27950 T27949 cc and,selected
R19359 T27951 T27949 conj maintained,selected
R19360 T27952 T27951 prep in,maintained
R19361 T27953 T27954 nummod 10,µg
R19362 T27954 T27957 compound µg,puromycin
R19363 T27955 T27957 compound /,puromycin
R19364 T27956 T27957 compound ml,puromycin
R19365 T27957 T27952 pobj puromycin,in
R19366 T27958 T27949 punct .,selected
R19451 T28070 T28072 compound ELISAOne,ELISAOne
R19452 T28071 T28072 compound Assay,ELISAOne
R19453 T28072 T28073 compound ELISAOne,assays
R19454 T28073 T28073 ROOT assays,assays
R19455 T28074 T28082 punct (,performed
R19456 T28075 T28082 nsubjpass TGRBio,performed
R19457 T28076 T28075 punct ",",TGRBio
R19458 T28077 T28075 conj Hindmarsh,TGRBio
R19459 T28078 T28077 punct ",",Hindmarsh
R19460 T28079 T28077 appos Australia,Hindmarsh
R19461 T28080 T28077 punct ),Hindmarsh
R19462 T28081 T28082 auxpass were,performed
R19463 T28082 T28073 relcl performed,assays
R19464 T28083 T28082 prep according,performed
R19465 T28084 T28083 prep to,according
R19466 T28085 T28086 poss manufacturer,protocol
R19467 T28086 T28084 pobj protocol,to
R19468 T28087 T28082 prep with,performed
R19469 T28088 T28090 det the,modifications
R19470 T28089 T28090 amod following,modifications
R19471 T28090 T28087 pobj modifications,with
R19472 T28091 T28082 punct .,performed
R19473 T28092 T28093 compound Cell,lysates
R19474 T28093 T28095 nsubjpass lysates,prepared
R19475 T28094 T28095 auxpass were,prepared
R19476 T28095 T28095 ROOT prepared,prepared
R19477 T28096 T28095 prep in,prepared
R19478 T28097 T28098 compound RIPA,buffer
R19479 T28098 T28096 pobj buffer,in
R19480 T28099 T28100 mark as,described
R19481 T28100 T28095 advcl described,prepared
R19482 T28101 T28100 prep for,described
R19483 T28102 T28103 amod Western,blots
R19484 T28103 T28101 pobj blots,for
R19485 T28104 T28095 punct .,prepared
R19486 T28105 T28106 nummod Five,microliters
R19487 T28106 T28110 nsubjpass microliters,diluted
R19488 T28107 T28106 prep of,microliters
R19489 T28108 T28107 pobj samples,of
R19490 T28109 T28110 auxpass were,diluted
R19491 T28110 T28110 ROOT diluted,diluted
R19492 T28111 T28110 prep in,diluted
R19493 T28112 T28113 nummod 45,µL
R19494 T28113 T28111 pobj µL,in
R19495 T28114 T28113 prep of,µL
R19496 T28115 T28116 compound ELISAOne,lysis
R19497 T28116 T28117 compound lysis,buffer
R19498 T28117 T28114 pobj buffer,of
R19499 T28118 T28110 advmod prior,diluted
R19500 T28119 T28118 prep to,prior
R19501 T28120 T28119 pobj analysis,to
R19502 T28121 T28110 punct .,diluted
R19503 T28122 T28123 amod Primary,antibodies
R19504 T28123 T28129 nsubjpass antibodies,incubated
R19505 T28124 T28123 prep to,antibodies
R19506 T28125 T28124 pobj phopsho-Thr308,to
R19507 T28126 T28125 cc and,phopsho-Thr308
R19508 T28127 T28125 conj phopsho-Ser473,phopsho-Thr308
R19509 T28128 T28129 auxpass were,incubated
R19510 T28129 T28129 ROOT incubated,incubated
R19511 T28130 T28129 prep with,incubated
R19512 T28131 T28132 det the,samples
R19513 T28132 T28130 pobj samples,with
R19514 T28133 T28132 prep for,samples
R19515 T28134 T28135 nummod 2,hr
R19516 T28135 T28133 pobj hr,for
R19517 T28136 T28129 prep at,incubated
R19518 T28137 T28138 compound room,temperature
R19519 T28138 T28136 pobj temperature,at
R19520 T28139 T28129 punct .,incubated
R19521 T28140 T28141 amod Primary,antibody
R19522 T28141 T28145 nsubjpass antibody,incubated
R19523 T28142 T28145 prep to,incubated
R19524 T28143 T28142 pobj pan-Akt,to
R19525 T28144 T28145 auxpass was,incubated
R19526 T28145 T28145 ROOT incubated,incubated
R19527 T28146 T28145 advmod overnight,incubated
R19528 T28147 T28145 prep at,incubated
R19529 T28148 T28149 nummod 4,°
R19530 T28149 T28151 nummod °,Signals
R19531 T28150 T28151 compound C.,Signals
R19532 T28151 T28147 pobj Signals,at
R19533 T28152 T28145 prep for,incubated
R19534 T28153 T28152 pobj phospho-antibodies,for
R19535 T28154 T28145 auxpass were,incubated
R19536 T28155 T28154 acomp normalized,were
R19537 T28156 T28155 prep based,normalized
R19538 T28157 T28156 prep on,based
R19539 T28158 T28159 amod pan-Akt,values
R19540 T28159 T28157 pobj values,on
R19541 T28160 T28145 punct .,incubated
R19576 T28229 T28234 compound TNFα,ELISA
R19577 T28230 T28234 compound ELISA,ELISA
R19578 T28231 T28234 compound Mouse,ELISA
R19579 T28232 T28234 compound TNFα,ELISA
R19580 T28233 T28234 compound Quantikine,ELISA
R19581 T28234 T28235 compound ELISA,assays
R19582 T28235 T28241 nsubjpass assays,performed
R19583 T28236 T28238 punct (,Systems
R19584 T28237 T28238 compound R&D,Systems
R19585 T28238 T28235 appos Systems,assays
R19586 T28239 T28235 punct ),assays
R19587 T28240 T28241 auxpass were,performed
R19588 T28241 T28241 ROOT performed,performed
R19589 T28242 T28241 prep according,performed
R19590 T28243 T28242 prep to,according
R19591 T28244 T28245 poss manufacturer,descriptions
R19592 T28245 T28243 pobj descriptions,to
R19593 T28246 T28241 punct .,performed
R19594 T28247 T28248 compound Cell,lysates
R19595 T28248 T28250 nsubjpass lysates,prepared
R19607 T28260 T28263 det a,dish
R19608 T28261 T28263 nummod 10,dish
R19609 T28262 T28263 compound cm2,dish
R19610 T28263 T28259 pobj dish,in
R19611 T28264 T28250 punct .,prepared
R19683 T28410 T28419 prep In,measured
R19684 T28411 T28417 amod vitro,activity
R19685 T28412 T28417 compound Akt,activity
R19686 T28413 T28417 compound Kinase,activity
R19687 T28414 T28417 compound Assay,activity
R19688 T28415 T28417 compound Akt,activity
R19689 T28416 T28417 compound kinase,activity
R19690 T28417 T28410 pobj activity,In
R19691 T28418 T28419 auxpass was,measured
R19692 T28419 T28419 ROOT measured,measured
R19693 T28420 T28419 advcl using,measured
R19694 T28421 T28425 det the,kit
R19695 T28422 T28425 compound Akt,kit
R19696 T28423 T28424 compound kinase,assay
R19697 T28424 T28425 compound assay,kit
R19698 T28425 T28420 dobj kit,using
R19699 T28426 T28425 punct (,kit
R19700 T28427 T28425 appos nonradioactive,kit
R19701 T28428 T28425 punct ),kit
R19702 T28429 T28420 prep from,using
R19703 T28430 T28432 compound Cell,Technology
R19704 T28431 T28432 compound Signaling,Technology
R19705 T28432 T28429 pobj Technology,from
R19706 T28433 T28419 punct .,measured
R19707 T28434 T28439 prep In,immunoprecipitated
R19708 T28435 T28434 pobj brief,In
R19709 T28436 T28439 punct ",",immunoprecipitated
R19710 T28437 T28439 nsubjpass Myr-Akt,immunoprecipitated
R19711 T28438 T28439 auxpass was,immunoprecipitated
R19712 T28439 T28439 ROOT immunoprecipitated,immunoprecipitated
R19713 T28440 T28439 prep from,immunoprecipitated
R19714 T28441 T28442 nummod L929,cells
R19715 T28442 T28440 pobj cells,from
R19716 T28443 T28439 advcl using,immunoprecipitated
R19717 T28444 T28447 amod anti-FLAG,beads
R19718 T28445 T28447 nmod M2,beads
R19719 T28446 T28447 amod magnetic,beads
R19720 T28447 T28443 dobj beads,using
R19721 T28448 T28447 punct (,beads
R19722 T28449 T28447 appos Sigma,beads
R19723 T28450 T28447 punct ),beads
R19724 T28451 T28439 punct .,immunoprecipitated
R19725 T28452 T28455 det The,assay
R19726 T28453 T28455 nmod in,assay
R19727 T28454 T28455 amod vitro,assay
R19728 T28455 T28457 nsubjpass assay,performed
R19729 T28456 T28457 auxpass was,performed
R19730 T28457 T28457 ROOT performed,performed
R19731 T28458 T28457 prep in,performed
R19732 T28459 T28460 det the,presence
R19733 T28460 T28458 pobj presence,in
R19734 T28461 T28460 prep of,presence
R19735 T28462 T28466 det a,substrate
R19736 T28463 T28465 compound GSK,protein
R19737 T28464 T28465 compound fusion,protein
R19738 T28465 T28466 compound protein,substrate
R19739 T28466 T28461 pobj substrate,of
R19740 T28467 T28457 punct .,performed
R19741 T28468 T28475 nsubjpass Phosphorylation,visualized
R19742 T28469 T28468 prep of,Phosphorylation
R19743 T28470 T28473 det the,protein
R19744 T28471 T28473 compound GSK,protein
R19745 T28472 T28473 compound fusion,protein
R19746 T28473 T28469 pobj protein,of
R19747 T28474 T28475 auxpass was,visualized
R19748 T28475 T28475 ROOT visualized,visualized
R19749 T28476 T28475 agent by,visualized
R19750 T28477 T28478 amod western,blot
R19751 T28478 T28476 pobj blot,by
R19752 T28479 T28475 punct .,visualized
R17873 T26139 T26140 punct :,reverse
R17876 T26142 T26144 punct ′,GAGGGCTGATTAGAGAGAGGTC-3
R17877 T26143 T26144 punct -,GAGGGCTGATTAGAGAGAGGTC-3
R17878 T26144 T26145 compound GAGGGCTGATTAGAGAGAGGTC-3,′
R17879 T26145 T26140 dep ′,reverse
R17880 T26146 T26160 punct ;,reverse
R17891 T26157 T26158 amod human,18S
R17892 T26158 T26148 appos 18S,18S
R17893 T26159 T26148 punct :,18S
R17894 T26160 T26160 ROOT reverse,reverse
R17895 T26161 T26165 nummod 5,′
R17896 T26162 T26164 punct ′,TAGTAGCGACGGGCGGTGTG-3
R17897 T26163 T26164 punct -,TAGTAGCGACGGGCGGTGTG-3
R17898 T26164 T26165 compound TAGTAGCGACGGGCGGTGTG-3,′
R17899 T26165 T26160 dobj ′,reverse
R17900 T26166 T26160 punct .,reverse
R18249 T26583 T26584 compound Cell,Viability
R18250 T26584 T26588 nsubjpass Viability,seeded
R18251 T26585 T26588 nsubjpass Experiments,seeded
R18292 T26626 T26628 nsubjpass Experiments,performed
R19596 T28249 T28250 auxpass were,prepared
R19597 T28250 T28250 ROOT prepared,prepared
R19598 T28251 T28250 prep from,prepared
R19599 T28252 T28255 nummod 3,cells
R19600 T28253 T28255 nummod ×,cells
R19601 T28254 T28255 nummod 106,cells
R19602 T28255 T28251 pobj cells,from
R19603 T28256 T28255 acl plated,cells
R19604 T28257 T28256 cc and,plated
R19605 T28258 T28256 conj treated,plated
R19606 T28259 T28258 prep in,treated
R19286 T27878 T27879 compound Stable,Infection
R19287 T27879 T27883 nsubj Infection,generate
R19288 T27880 T27879 prep of,Infection
R19289 T27881 T27880 pobj Myr-Akt1,of
R19290 T27882 T27883 aux To,generate
R19291 T27883 T27883 ROOT generate,generate
R19292 T27884 T27885 compound MSCV,retroviruses
R19293 T27885 T27883 dobj retroviruses,generate
R19294 T27886 T27885 punct ",",retroviruses
R19295 T27887 T27888 amod HEK293FT,cells
R19296 T27888 T27885 conj cells,retroviruses
R19297 T27889 T27890 punct (,Invitrogen
R19344 T27936 T27930 conj applied,filtered
R19345 T27937 T27936 prep to,applied
R18567 T27010 T27003 punct ",",transfected
R18568 T27011 T27003 prep according,transfected
R17521 T25679 T25676 dobj tag,containing
R17522 T25680 T25683 punct ",",described
R17523 T25681 T25683 aux has,described
R17524 T25682 T25683 auxpass been,described
R17525 T25683 T25683 ROOT described,described
R17526 T25684 T25683 dobj [,described
R17527 T25685 T25686 nummod 52,]
R17528 T25686 T25684 appos ],[
R17529 T25687 T25683 punct .,described
R18569 T27012 T27011 prep to,according
R18542 T26985 T26986 punct (,J-040128-05
R18543 T26986 T26984 appos J-040128-05,Mapk8
R18544 T26987 T26984 punct ),Mapk8
R18545 T26988 T26984 punct ",",Mapk8
R18546 T26989 T26990 compound mouse,Mapk9
R18547 T26990 T26984 conj Mapk9,Mapk8
R18548 T26991 T26992 punct (,J-040134-05
R18549 T26992 T26990 appos J-040134-05,Mapk9
R18550 T26993 T26992 punct ),J-040134-05
R18551 T26994 T26990 punct ",",Mapk9
R18552 T26995 T26996 compound mouse,Jun
R18553 T26996 T26990 appos Jun,Mapk9
R18554 T26997 T26998 punct (,L-043776-00
R18555 T26998 T26996 parataxis L-043776-00,Jun
R18556 T26999 T26998 punct ),L-043776-00
R18557 T27000 T26940 punct .,protein
R18558 T27001 T27003 nsubjpass siRNA,transfected
R18559 T27002 T27003 auxpass were,transfected
R18560 T27003 T27003 ROOT transfected,transfected
R18561 T27004 T27003 advcl using,transfected
R18562 T27005 T27006 compound RNAiMAX,reagent
R18563 T27006 T27004 dobj reagent,using
R18564 T27007 T27006 punct (,reagent
R18565 T27008 T27006 appos Invitrogen,reagent
R18566 T27009 T27008 punct ),Invitrogen
R18982 T27530 T27529 cc or,milk
R17513 T25671 T25672 compound DNA,Cloning
R17514 T25672 T25683 nsubj Cloning,described
R17515 T25673 T25672 prep of,Cloning
R17516 T25674 T25673 pobj Myr-Akt1,of
R17517 T25675 T25683 punct ",",described
R17518 T25676 T25683 advcl containing,described
R17519 T25677 T25679 amod c-terminal,tag
R17520 T25678 T25679 compound FLAG,tag
R17530 T25688 T25690 nsubjpass Myr-Akt1-FLAG,amplified
R17531 T25689 T25690 auxpass was,amplified
R17532 T25690 T25690 ROOT amplified,amplified
R17870 T26136 T26135 punct ",",′
R17871 T26137 T26138 compound human,TNFα

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T28388 7731-7746 Phosphorylation denotes Phosphorylation
T28387 7754-7772 Protein denotes GSK fusion protein
T28386 7701-7719 Protein denotes GSK fusion protein
T28385 7561-7564 Protein denotes Akt
T28384 7557-7560 Protein denotes Myr
T28383 7477-7487 Protein denotes Akt kinase
T28382 7434-7444 Protein denotes Akt kinase
T28381 7417-7427 Protein denotes Akt Kinase
T28228 7220-7247 Protein denotes Mouse TNFα Quantikine ELISA
T28227 7209-7219 Protein denotes TNFα ELISA
T27864 6350-6353 Protein denotes pol
T27863 6346-6349 Protein denotes gal
T27862 6226-6234 Protein denotes Myr-Akt1
T27332 5444-5447 Protein denotes BSA
T27331 5429-5442 Protein denotes serum albumin
T27330 4936-4940 Protein denotes TNFα
T27329 4906-4917 Protein denotes µM zVAD.fmk
T27328 4777-4781 Protein denotes TNFα
T27327 4747-4758 Protein denotes µM zVAD.fmk
T27687 6137-6159 Protein denotes SYBRGreen 2×Master mix
T26091 2567-2577 Protein denotes human TNFα
T26090 2522-2526 Protein denotes TNFα
T26089 2341-2351 Protein denotes Mouse TNFα
T26907 4520-4536 Protein denotes zVAD.fmk or TNFα
T26906 4365-4376 Entity denotes L-043776-00
T26905 4274-4281 Regulation denotes control
T26904 4223-4234 Entity denotes L-065427-00
T26903 4103-4114 Entity denotes L-040893-00
T26902 4333-4338 Protein denotes Mapk9
T26901 4306-4311 Protein denotes Mapk8
T26900 4243-4247 Protein denotes PDK1
T26899 4191-4195 Protein denotes Akt3
T26898 4165-4169 Protein denotes Akt2
T26897 4139-4143 Protein denotes Akt1
T26896 4075-4101 Protein denotes Mouse ribosomal S6 protein
T25543 1012-1017 Protein denotes IGF-1
T25542 988-995 Protein denotes PDGF-BB
T25541 972-975 Protein denotes EGF
T25540 949-959 Protein denotes human bFGF
T25539 932-936 Protein denotes TNFα
T25538 851-858 Protein denotes caspase
T25537 847-850 Protein denotes Pan
T25536 250-259 Negative_regulation denotes inhibitor
T25535 400-409 Negative_regulation denotes inhibitor
T25534 250-259 Negative_regulation denotes inhibitor
T25533 396-399 Protein denotes JNK
T25532 246-249 Protein denotes Akt
R17458 T25532 T25534 themeOf Akt,inhibitor
R17459 T25533 T25535 themeOf JNK,inhibitor
R17460 T25535 T25536 themeOf inhibitor,inhibitor
R18476 T26896 T26903 partOf Mouse ribosomal S6 protein,L-040893-00
R18477 T26897 T26905 themeOf Akt1,control
R18478 T26897 T26903 partOf Akt1,L-040893-00
R18479 T26897 T26904 partOf Akt1,L-065427-00
R18480 T26897 T26906 partOf Akt1,L-043776-00
R19681 T28387 T28388 themeOf GSK fusion protein,Phosphorylation

test3

Id Subject Object Predicate Lexical cue
T28168 7226-7230 Protein denotes TNFα
T28167 7209-7213 Protein denotes TNFα
T28166 7226-7230 Protein denotes TNFα
T28165 7209-7213 Protein denotes TNFα
T28278 7557-7564 Protein denotes Myr-Akt
T28277 7557-7564 Protein denotes Myr-Akt
T27773 6226-6234 Protein denotes Myr-Akt1
T27058 4936-4940 Protein denotes TNFα
T27057 4777-4781 Protein denotes TNFα
T27056 4936-4940 Protein denotes TNFα
T27055 4777-4781 Protein denotes TNFα
T26719 4532-4536 Protein denotes TNFα
T26718 4333-4338 Protein denotes Mapk9
T26717 4306-4311 Protein denotes Mapk8
T26716 4243-4247 Protein denotes PDK1
T26715 4191-4195 Protein denotes Akt3
T26714 4165-4169 Protein denotes Akt2
T26713 4139-4143 Protein denotes Akt1
T26712 4532-4536 Protein denotes TNFα
T26711 4333-4338 Protein denotes Mapk9
T26710 4306-4311 Protein denotes Mapk8
T26709 4243-4247 Protein denotes PDK1
T26708 4191-4195 Protein denotes Akt3
T26707 4165-4169 Protein denotes Akt2
T26706 4139-4143 Protein denotes Akt1
T26187 2738-2747 Negative_regulation denotes deficient
T26186 2733-2737 Protein denotes FADD
T26185 2733-2737 Protein denotes FADD
T25988 2671-2674 Protein denotes 18S
T25987 2623-2626 Protein denotes 18S
T25986 2573-2577 Protein denotes TNFα
T25985 2522-2526 Protein denotes TNFα
T25984 2432-2435 Protein denotes 18S
T25983 2347-2351 Protein denotes TNFα
T25982 2671-2674 Protein denotes 18S
T25981 2623-2626 Protein denotes 18S
T25980 2573-2577 Protein denotes TNFα
T25979 2522-2526 Protein denotes TNFα
T25978 2432-2435 Protein denotes 18S
T25977 2347-2351 Protein denotes TNFα
T25585 1331-1339 Protein denotes Myr-Akt1
T25584 1186-1199 Protein denotes Myr-Akt1-FLAG
T25583 1119-1127 Protein denotes Myr-Akt1
T25582 1331-1339 Protein denotes Myr-Akt1
T25581 1186-1199 Protein denotes Myr-Akt1-FLAG
T25580 1119-1127 Protein denotes Myr-Akt1
T25128 1012-1017 Protein denotes IGF-1
T25127 972-975 Protein denotes EGF
T25126 955-959 Protein denotes bFGF
T25125 932-936 Protein denotes TNFα
T25124 250-259 Negative_regulation denotes inhibitor
T25123 1012-1017 Protein denotes IGF-1
T25122 972-975 Protein denotes EGF
T25121 955-959 Protein denotes bFGF
T25120 932-936 Protein denotes TNFα
R17902 T26186 T26187 themeOf FADD,deficient