PMC:3585731 / 24387-25093 JSONTXT

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Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T30689","span":{"begin":355,"end":359},"obj":"Protein"},{"id":"T30688","span":{"begin":303,"end":306},"obj":"Protein"},{"id":"T30687","span":{"begin":267,"end":272},"obj":"Protein"},{"id":"T30686","span":{"begin":187,"end":191},"obj":"Protein"},{"id":"T30685","span":{"begin":77,"end":81},"obj":"Protein"},{"id":"T30692","span":{"begin":633,"end":638},"obj":"Protein"},{"id":"T30691","span":{"begin":586,"end":590},"obj":"Protein"},{"id":"T30690","span":{"begin":461,"end":465},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T30885","span":{"begin":542,"end":546},"obj":"http://www.uniprot.org/uniprot/P42345"},{"id":"T30884","span":{"begin":187,"end":191},"obj":"http://www.uniprot.org/uniprot/Q788Q8"},{"id":"T30883","span":{"begin":586,"end":590},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T30882","span":{"begin":461,"end":465},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T30881","span":{"begin":355,"end":359},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T30880","span":{"begin":77,"end":81},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T30879","span":{"begin":520,"end":523},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T30878","span":{"begin":410,"end":413},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T30877","span":{"begin":44,"end":47},"obj":"http://www.uniprot.org/uniprot/Q9Y243"},{"id":"T30876","span":{"begin":520,"end":523},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T30875","span":{"begin":410,"end":413},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T30874","span":{"begin":44,"end":47},"obj":"http://www.uniprot.org/uniprot/P31751"},{"id":"T30873","span":{"begin":520,"end":523},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T30872","span":{"begin":410,"end":413},"obj":"http://www.uniprot.org/uniprot/P31749"},{"id":"T30871","span":{"begin":44,"end":47},"obj":"http://www.uniprot.org/uniprot/P31749"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"UBERON-AE","denotations":[{"id":"T30669","span":{"begin":160,"end":165},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"GO-BP","denotations":[{"id":"T30698","span":{"begin":316,"end":327},"obj":"http://purl.obolibrary.org/obo/GO_0097528"},{"id":"T30697","span":{"begin":118,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0097528"},{"id":"T30696","span":{"begin":316,"end":327},"obj":"http://purl.obolibrary.org/obo/GO_0070266"},{"id":"T30695","span":{"begin":118,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0070266"},{"id":"T30694","span":{"begin":96,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0004705"},{"id":"T30693","span":{"begin":82,"end":91},"obj":"http://purl.obolibrary.org/obo/GO_0009058"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"GO-MF","denotations":[{"id":"T30700","span":{"begin":195,"end":199},"obj":"http://purl.obolibrary.org/obo/GO_0005161"},{"id":"T30699","span":{"begin":96,"end":99},"obj":"http://purl.obolibrary.org/obo/GO_0004705"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"GO-CC","denotations":[{"id":"T30703","span":{"begin":490,"end":495},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T30702","span":{"begin":363,"end":368},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T30701","span":{"begin":52,"end":58},"obj":"http://purl.obolibrary.org/obo/GO_0031931"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"sentences","denotations":[{"id":"T30674","span":{"begin":668,"end":706},"obj":"Sentence"},{"id":"T30673","span":{"begin":419,"end":667},"obj":"Sentence"},{"id":"T30672","span":{"begin":274,"end":418},"obj":"Sentence"},{"id":"T30671","span":{"begin":131,"end":273},"obj":"Sentence"},{"id":"T30670","span":{"begin":44,"end":130},"obj":"Sentence"},{"id":"T156","span":{"begin":0,"end":130},"obj":"Sentence"},{"id":"T157","span":{"begin":131,"end":273},"obj":"Sentence"},{"id":"T158","span":{"begin":274,"end":418},"obj":"Sentence"},{"id":"T159","span":{"begin":419,"end":667},"obj":"Sentence"},{"id":"T160","span":{"begin":668,"end":706},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"simple1","denotations":[{"id":"T30719","span":{"begin":633,"end":638},"obj":"Protein"},{"id":"T30718","span":{"begin":586,"end":590},"obj":"Protein"},{"id":"T30717","span":{"begin":461,"end":465},"obj":"Protein"},{"id":"T30716","span":{"begin":355,"end":359},"obj":"Protein"},{"id":"T30715","span":{"begin":303,"end":306},"obj":"Protein"},{"id":"T30714","span":{"begin":267,"end":272},"obj":"Protein"},{"id":"T30713","span":{"begin":187,"end":191},"obj":"Protein"},{"id":"T30712","span":{"begin":77,"end":81},"obj":"Protein"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T30870","span":{"begin":471,"end":477},"obj":"Transcription"},{"id":"T30869","span":{"begin":59,"end":66},"obj":"Regulation"},{"id":"T30868","span":{"begin":82,"end":91},"obj":"Gene_expression"},{"id":"T30867","span":{"begin":633,"end":638},"obj":"Protein"},{"id":"T30866","span":{"begin":586,"end":590},"obj":"Protein"},{"id":"T30865","span":{"begin":461,"end":465},"obj":"Protein"},{"id":"T30864","span":{"begin":355,"end":359},"obj":"Protein"},{"id":"T30863","span":{"begin":303,"end":306},"obj":"Protein"},{"id":"T30862","span":{"begin":267,"end":272},"obj":"Protein"},{"id":"T30861","span":{"begin":187,"end":191},"obj":"Protein"},{"id":"T30860","span":{"begin":77,"end":81},"obj":"Protein"}],"relations":[{"id":"R21353","pred":"themeOf","subj":"T30860","obj":"T30868"},{"id":"R21354","pred":"themeOf","subj":"T30865","obj":"T30870"},{"id":"R21355","pred":"themeOf","subj":"T30868","obj":"T30869"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T30915","span":{"begin":332,"end":339},"obj":"Positive_regulation"},{"id":"T30914","span":{"begin":82,"end":91},"obj":"Gene_expression"},{"id":"T30913","span":{"begin":67,"end":76},"obj":"Positive_regulation"},{"id":"T30912","span":{"begin":633,"end":638},"obj":"Protein"},{"id":"T30911","span":{"begin":586,"end":590},"obj":"Protein"},{"id":"T30910","span":{"begin":461,"end":465},"obj":"Protein"},{"id":"T30909","span":{"begin":355,"end":359},"obj":"Protein"},{"id":"T30908","span":{"begin":303,"end":306},"obj":"Protein"},{"id":"T30907","span":{"begin":267,"end":272},"obj":"Protein"},{"id":"T30906","span":{"begin":187,"end":191},"obj":"Protein"},{"id":"T30905","span":{"begin":77,"end":81},"obj":"Protein"}],"relations":[{"id":"R21359","pred":"themeOf","subj":"T30905","obj":"T30914"},{"id":"R21360","pred":"themeOf","subj":"T30909","obj":"T30915"},{"id":"R21361","pred":"themeOf","subj":"T30914","obj":"T30913"}],"text":"10.1371/journal.pone.0056576.g006 Figure 6 Akt and mTORC1 control autocrine TNFα synthesis and JNK activation during necroptosis.\n(A) Cells were treated under serum free conditions with bFGF or PDGF with or without zVAD.fmk for 9 hr, followed by qRT-PCR analysis of mTNFα. Data was normalized to mouse 18S RNA. (B) Necroptosis was induced by zVAD.fmk or TNFα in cells treated with Nec-1, rapamycin (rapa), or Akt inh. VIII inh. followed by qRT-PCR analysis of TNFα mRNA levels. (C-F) L929 cells with siRNA knockdown of Akt isoforms (C,E) or mTOR (D,F) were stimulated with zVAD.fmk or TNFα for 9 hr, followed by qRT-PCR analysis of mTNFα (C,D) or western blot (E,F). In all graphs, average±SD was plotted."}

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