PMC:3585731 / 13176-13740 JSONTXT

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Figure 3 RIP1 kinase-dependent phosphorylation of Akt and JNK during necroptosis.\n(A) L929 cells were treated with zVAD.fmk or TNFα for 9 hr, followed by western blotting with indicated antibodies. (B,C) L929 cells were treated with zVAD.fmk (B) or bFGF/zVAD.fmk (serum free conditions, C) and samples were collected at the indicated time points for western blot. (D) Nec-1 was added to the cells stimulated with bFGF or bFGF/zVAD (serum free conditions) for 15 min or 9 hr followed by western blot with the indicated antibodies."}

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Figure 3 RIP1 kinase-dependent phosphorylation of Akt and JNK during necroptosis.\n(A) L929 cells were treated with zVAD.fmk or TNFα for 9 hr, followed by western blotting with indicated antibodies. (B,C) L929 cells were treated with zVAD.fmk (B) or bFGF/zVAD.fmk (serum free conditions, C) and samples were collected at the indicated time points for western blot. (D) Nec-1 was added to the cells stimulated with bFGF or bFGF/zVAD (serum free conditions) for 15 min or 9 hr followed by western blot with the indicated antibodies."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T29689","span":{"begin":456,"end":465},"obj":"Protein"},{"id":"T29688","span":{"begin":448,"end":452},"obj":"Protein"},{"id":"T29687","span":{"begin":284,"end":297},"obj":"Protein"},{"id":"T29686","span":{"begin":162,"end":166},"obj":"Protein"},{"id":"T29685","span":{"begin":150,"end":158},"obj":"Protein"},{"id":"T29684","span":{"begin":56,"end":65},"obj":"Positive_regulation"},{"id":"T29683","span":{"begin":56,"end":65},"obj":"Positive_regulation"},{"id":"T29682","span":{"begin":66,"end":81},"obj":"Phosphorylation"},{"id":"T29681","span":{"begin":66,"end":81},"obj":"Phosphorylation"},{"id":"T29680","span":{"begin":93,"end":96},"obj":"Protein"},{"id":"T29679","span":{"begin":85,"end":88},"obj":"Protein"},{"id":"T29678","span":{"begin":44,"end":55},"obj":"Protein"}],"relations":[{"id":"R20570","pred":"causeOf","subj":"T29678","obj":"T29683"},{"id":"R20571","pred":"causeOf","subj":"T29678","obj":"T29684"},{"id":"R20572","pred":"themeOf","subj":"T29679","obj":"T29681"},{"id":"R20573","pred":"themeOf","subj":"T29680","obj":"T29682"},{"id":"R20574","pred":"themeOf","subj":"T29681","obj":"T29683"},{"id":"R20575","pred":"themeOf","subj":"T29682","obj":"T29684"}],"text":"10.1371/journal.pone.0056576.g003 Figure 3 RIP1 kinase-dependent phosphorylation of Akt and JNK during necroptosis.\n(A) L929 cells were treated with zVAD.fmk or TNFα for 9 hr, followed by western blotting with indicated antibodies. (B,C) L929 cells were treated with zVAD.fmk (B) or bFGF/zVAD.fmk (serum free conditions, C) and samples were collected at the indicated time points for western blot. (D) Nec-1 was added to the cells stimulated with bFGF or bFGF/zVAD (serum free conditions) for 15 min or 9 hr followed by western blot with the indicated antibodies."}

{"project":"test3","denotations":[{"id":"T29398","span":{"begin":448,"end":452},"obj":"Protein"},{"id":"T29397","span":{"begin":284,"end":288},"obj":"Protein"},{"id":"T29396","span":{"begin":162,"end":166},"obj":"Protein"},{"id":"T29395","span":{"begin":66,"end":81},"obj":"Phosphorylation"},{"id":"T29394","span":{"begin":44,"end":48},"obj":"Protein"},{"id":"T29393","span":{"begin":456,"end":460},"obj":"Protein"},{"id":"T29392","span":{"begin":448,"end":452},"obj":"Protein"},{"id":"T29391","span":{"begin":284,"end":288},"obj":"Protein"},{"id":"T29390","span":{"begin":162,"end":166},"obj":"Protein"},{"id":"T29389","span":{"begin":44,"end":48},"obj":"Protein"},{"id":"T29399","span":{"begin":456,"end":460},"obj":"Protein"}],"text":"10.1371/journal.pone.0056576.g003 Figure 3 RIP1 kinase-dependent phosphorylation of Akt and JNK during necroptosis.\n(A) L929 cells were treated with zVAD.fmk or TNFα for 9 hr, followed by western blotting with indicated antibodies. (B,C) L929 cells were treated with zVAD.fmk (B) or bFGF/zVAD.fmk (serum free conditions, C) and samples were collected at the indicated time points for western blot. (D) Nec-1 was added to the cells stimulated with bFGF or bFGF/zVAD (serum free conditions) for 15 min or 9 hr followed by western blot with the indicated antibodies."}