PMC:3558958 / 10300-10756 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3558958","sourcedb":"PMC","sourceid":"3558958","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3558958","text":"Growth conditions and DNA Isolation\nFor preparation of genomic DNA, liter cultures of Y412MC10 were grown from a single colony in YTP-2 medium at 37°C in flasks agitated at 200 rpm and collected by centrifugation. The cell concentrate was lysed using a combination of SDS and proteinase K, and genomic DNA was isolated using a phenol/chloroform extraction [43]. The genomic DNA was precipitated, and treated with RNase to remove residual contaminating RNA.","divisions":[{"label":"Title","span":{"begin":0,"end":35}}],"tracks":[{"project":"MicrobeTaxon","denotations":[{"id":"T71","span":{"begin":86,"end":94},"obj":"481743"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"attributes":[{"subj":"T71","pred":"source","obj":"MicrobeTaxon"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"MicrobeTaxon","color":"#ecb393","default":true}]}]}}