PMC:3558958 / 10300-10756 JSONTXT

{"project":"MicrobeTaxon","denotations":[{"id":"T71","span":{"begin":86,"end":94},"obj":"481743"}],"namespaces":[{"prefix":"_base","uri":"http://purl.bioontology.org/ontology/NCBITAXON/"}],"text":"Growth conditions and DNA Isolation\nFor preparation of genomic DNA, liter cultures of Y412MC10 were grown from a single colony in YTP-2 medium at 37°C in flasks agitated at 200 rpm and collected by centrifugation. The cell concentrate was lysed using a combination of SDS and proteinase K, and genomic DNA was isolated using a phenol/chloroform extraction [43]. The genomic DNA was precipitated, and treated with RNase to remove residual contaminating RNA."}