PMC:3492202 / 9015-9455 JSONTXT

{"project":"2_test","denotations":[{"id":"23062147-21093485-143781707","span":{"begin":160,"end":162},"obj":"21093485"},{"id":"23062147-17940950-143781708","span":{"begin":163,"end":165},"obj":"17940950"}],"text":"The Invitrogen OneStep RT-PCR Kit was used to screen ebolavirus RNA using the universal primers against the Filovirus L gene or N gene as described previously [12,13]. RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions. No filovirus-specific RNA was detected by one step RT-PCR among 143 tissue samples (spleen, liver or fecal swab) tested, therefore, virus isolation was not attempted."}