The Invitrogen OneStep RT-PCR Kit was used to screen ebolavirus RNA using the universal primers against the Filovirus L gene or N gene as described previously [12,13]. RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions. No filovirus-specific RNA was detected by one step RT-PCR among 143 tissue samples (spleen, liver or fecal swab) tested, therefore, virus isolation was not attempted.