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neuron cultures were prepared as previously reported [6]. In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero. The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies). The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below. The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2. Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent. The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C. After remove of the media with cellular debris, the cells were used for coating coverslips."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T5189","span":{"begin":756,"end":776},"obj":"CHEBI:28680"},{"id":"T5188","span":{"begin":702,"end":705},"obj":"CHEBI:16526"},{"id":"T5187","span":{"begin":635,"end":640},"obj":"GO:0040007"},{"id":"T5186","span":{"begin":621,"end":629},"obj":"UBERON:0001851"},{"id":"T5185","span":{"begin":615,"end":620},"obj":"NCBITaxon:10088"},{"id":"T5184","span":{"begin":570,"end":589},"obj":"CL:0002605"},{"id":"T5183","span":{"begin":570,"end":578},"obj":"UBERON:0001851"},{"id":"T5182","span":{"begin":565,"end":568},"obj":"CHEBI:16526"},{"id":"T5181","span":{"begin":498,"end":505},"obj":"CL:0000540"},{"id":"T5180","span":{"begin":445,"end":464},"obj":"CL:0002605"},{"id":"T5179","span":{"begin":445,"end":453},"obj":"UBERON:0001851"},{"id":"T5178","span":{"begin":439,"end":444},"obj":"NCBITaxon:10088"},{"id":"T5177","span":{"begin":350,"end":369},"obj":"CL:0002608"},{"id":"T5176","span":{"begin":305,"end":312},"obj":"PR:000027795"},{"id":"T5175","span":{"begin":161,"end":168},"obj":"UBERON:0000479"},{"id":"T5174","span":{"begin":128,"end":135},"obj":"UBERON:0000922"},{"id":"T5173","span":{"begin":122,"end":127},"obj":"NCBITaxon:10088"},{"id":"T5172","span":{"begin":0,"end":18},"obj":"CL:0002608"}],"text":"Hippocampal neuron cultures were prepared as previously reported [6]. In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero. The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies). The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below. The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2. Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent. The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C. After remove of the media with cellular debris, the cells were used for coating coverslips."}

{"project":"2_test","denotations":[{"id":"14723793-8657283-10642200","span":{"begin":66,"end":67},"obj":"8657283"}],"text":"Hippocampal neuron cultures were prepared as previously reported [6]. In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero. The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies). The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below. The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2. Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent. The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C. After remove of the media with cellular debris, the cells were used for coating coverslips."}

{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T5234","span":{"begin":884,"end":889},"obj":"CL_GO_EXT:cell"},{"id":"T5233","span":{"begin":863,"end":871},"obj":"CL_GO_EXT:cell"},{"id":"T5232","span":{"begin":756,"end":776},"obj":"CHEBI:28680"},{"id":"T5231","span":{"begin":727,"end":732},"obj":"CL_GO_EXT:cell"},{"id":"T5230","span":{"begin":702,"end":705},"obj":"CHEBI:16526"},{"id":"T5229","span":{"begin":635,"end":640},"obj":"GO:0040007"},{"id":"T5228","span":{"begin":621,"end":629},"obj":"UBERON:0001851"},{"id":"T5227","span":{"begin":615,"end":620},"obj":"NCBITaxon:10088"},{"id":"T5226","span":{"begin":607,"end":614},"obj":"PATO_UBERON_EXT:neonate_or_newborn"},{"id":"T5225","span":{"begin":570,"end":589},"obj":"CL:0002605"},{"id":"T5224","span":{"begin":570,"end":578},"obj":"UBERON:0001851"},{"id":"T5223","span":{"begin":565,"end":568},"obj":"CHEBI:16526"},{"id":"T5222","span":{"begin":498,"end":505},"obj":"CL:0000540"},{"id":"T5221","span":{"begin":445,"end":464},"obj":"CL:0002605"},{"id":"T5220","span":{"begin":445,"end":453},"obj":"UBERON:0001851"},{"id":"T5219","span":{"begin":439,"end":444},"obj":"NCBITaxon:10088"},{"id":"T5218","span":{"begin":350,"end":369},"obj":"CL:0002608"},{"id":"T5217","span":{"begin":350,"end":361},"obj":"UBERON_EXT:hippocampus_proper_or_hippocampal_formation"},{"id":"T5216","span":{"begin":305,"end":312},"obj":"PR_EXT:000027795"},{"id":"T5215","span":{"begin":161,"end":168},"obj":"UBERON:0000479"},{"id":"T5214","span":{"begin":128,"end":135},"obj":"UBERON:0000922"},{"id":"T5213","span":{"begin":122,"end":127},"obj":"NCBITaxon:10088"},{"id":"T5212","span":{"begin":84,"end":97},"obj":"UBERON_EXT:hippocampus_proper_or_hippocampal_formation"},{"id":"T5211","span":{"begin":0,"end":18},"obj":"CL:0002608"},{"id":"T5210","span":{"begin":0,"end":11},"obj":"UBERON_EXT:hippocampus_proper_or_hippocampal_formation"}],"text":"Hippocampal neuron cultures were prepared as previously reported [6]. In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero. The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies). The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below. The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2. Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent. The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C. After remove of the media with cellular debris, the cells were used for coating coverslips."}