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PMC:340383 / 17882-23326 JSONTXT

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Id Subject Object Predicate Lexical cue
T5718 5442-5443 -RRB- denotes )
T5717 5437-5442 NN denotes Acdp4
T5716 5436-5437 -LRB- denotes (
T5715 5427-5435 NN denotes AF216963
T5714 5423-5426 CC denotes and
T5713 5421-5422 -RRB- denotes )
T5701 5379-5380 -LRB- denotes (
T5700 5362-5369 NNS denotes numbers
T5699 5370-5378 NN denotes AF202994
T5698 5352-5361 NN denotes accession
T5697 5347-5351 IN denotes with
T5696 5342-5346 NN denotes bank
T5695 5337-5341 NN denotes gene
T5694 5334-5336 IN denotes in
T5693 5319-5323 VBN denotes been
T5692 5311-5318 RB denotes already
T5691 5306-5310 VBP denotes have
T5690 5294-5298 NN denotes gene
T5689 5289-5293 NN denotes Acdp
T5688 5299-5305 NN denotes family
T5687 5285-5288 DT denotes the
T5686 5281-5284 IN denotes for
T5685 5324-5333 VBN denotes deposited
T5684 5266-5270 NN denotes cDNA
T5683 5271-5280 NNS denotes sequences
T5682 5262-5265 DT denotes The
T5681 5261-5444 sentence denotes The cDNA sequences for the Acdp gene family have already been deposited in gene bank with accession numbers AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5680 5245-5254 NN denotes accession
T5678 5240-5244 NN denotes bank
T5677 5235-5239 NN denotes Gene
T5370 3860-3870 NN denotes atmosphere
T5369 3847-3848 DT denotes a
T5368 3844-3846 IN denotes in
T5367 3841-3843 NN denotes °C
T5366 3839-3841 CD denotes 37
T5365 3836-3838 IN denotes at
T5364 3820-3824 VBD denotes were
T5363 3825-3835 VBN denotes maintained
T5362 3812-3819 NNS denotes neurons
T5361 3808-3811 DT denotes The
T5360 3807-3883 sentence denotes The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2.
T5359 3806-3807 . denotes .
T5358 3801-3806 RB denotes below
T5357 3791-3800 VBN denotes described
T5356 3788-3790 IN denotes as
T5355 3779-3787 VBN denotes obtained
T5354 3759-3767 JJ denotes cortical
T5353 3768-3778 NNS denotes astrocytes
T5352 3753-3758 NN denotes mouse
T5351 3750-3752 IN denotes of
T5350 3730-3739 JJ denotes confluent
T5349 3740-3749 NN denotes monolayer
T5348 3728-3729 DT denotes a
T5347 3723-3727 IN denotes with
T5346 3716-3722 VBN denotes coated
T5345 3705-3715 NNS denotes coverslips
T5344 3699-3704 NN denotes glass
T5343 3696-3698 IN denotes on
T5342 3684-3688 VBD denotes were
T5341 3689-3695 VBN denotes plated
T5340 3664-3675 JJ denotes hippocampal
T5339 3652-3663 JJ denotes dissociated
T5338 3676-3683 NNS denotes neurons
T5337 3648-3651 DT denotes The
T5336 3647-3807 sentence denotes The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below.
T5335 3646-3647 . denotes .
T5334 3645-3646 -RRB- denotes )
T5333 3628-3632 NNP denotes Life
T5332 3633-3645 NNP denotes Technologies
T5331 3627-3628 -LRB- denotes (
T5330 3619-3626 NN denotes trypsin
T5329 3617-3618 NN denotes %
T5328 3613-3617 CD denotes 0.25
T5327 3608-3612 CC denotes plus
T5326 3606-3607 -RRB- denotes )
T5325 3589-3593 NNP denotes Life
T5324 3594-3606 NNP denotes Technologies
T5323 3588-3589 -LRB- denotes (
T5322 3580-3587 NN denotes culture
T5321 3569-3579 NN denotes suspension
T5320 3565-3568 IN denotes for
T5319 3556-3564 VBN denotes modified
T5318 3554-3555 -RRB- denotes )
T5317 3538-3547 JJ denotes essential
T5316 3548-3554 NN denotes medium
T5315 3530-3537 JJ denotes minimum
T5314 3529-3530 -LRB- denotes (
T5313 3525-3528 NN denotes MEM
T5312 3522-3524 IN denotes in
T5311 3519-3521 NN denotes °C
T5310 3517-3519 CD denotes 37
T5309 3514-3516 IN denotes at
T5308 3510-3513 NN denotes min
T5307 3507-3509 CD denotes 20
T5306 3503-3506 IN denotes for
T5305 3488-3492 RB denotes then
T5304 3483-3487 VBD denotes were
T5303 3493-3502 VBN denotes incubated
T5302 3475-3482 NNS denotes tissues
T5301 3471-3474 DT denotes The
T5300 3470-3647 sentence denotes The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).
T5299 3469-3470 . denotes .
T5298 3464-3469 FW denotes utero
T5297 3461-3463 FW denotes in
T5296 3456-3460 NNS denotes days
T5295 3453-3455 CD denotes 16
T5294 3450-3452 IN denotes at
T5293 3442-3449 NNS denotes embryos
T5292 3436-3441 NN denotes mouse
T5291 3431-3435 IN denotes from
T5290 3427-3430 RP denotes out
T5289 3412-3416 VBD denotes were
T5288 3398-3411 NNS denotes hippocampuses
T5287 3394-3397 DT denotes the
T5286 3392-3394 , denotes ,
T5285 3387-3392 NN denotes brief
T5284 3417-3426 VBN denotes dissected
T5283 3384-3386 IN denotes In
T5282 3383-3470 sentence denotes In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero.
T5281 3382-3383 . denotes .
T5280 3381-3382 -RRB- denotes ]
T5279 3380-3381 CD denotes 6
T5278 3379-3380 -LRB- denotes [
T5277 3359-3369 RB denotes previously
T5276 3370-3378 VBN denotes reported
T5275 3356-3358 IN denotes as
T5274 3342-3346 VBD denotes were
T5273 3347-3355 VBN denotes prepared
T5272 3326-3332 NN denotes neuron
T5271 3333-3341 NNS denotes cultures
T5270 3314-3325 JJ denotes Hippocampal
T5269 3313-3383 sentence denotes Hippocampal neuron cultures were prepared as previously reported [6].
T5268 3298-3313 NN denotes immunostanining
T5267 3294-3297 CC denotes and
T5266 3277-3281 NN denotes cell
T5265 3282-3293 NN denotes preparation
T5264 3268-3276 JJ denotes Neuronal
T5100 3126-3130 NN denotes tree
T5099 3113-3125 JJ denotes Phylogenetic
T5098 3112-3266 sentence denotes Phylogenetic tree was constructed by Clustalw program (version 1.81) using UPGMA (Unweighted Pair Group Method using Arithmetic averages) algorithm [14].
T5097 3111-3112 . denotes .
T5096 3104-3111 NN denotes protein
T5095 3098-3103 JJ denotes known
T5094 3095-3097 IN denotes of
T5093 3086-3094 NNS denotes features
T5092 3077-3085 NN denotes sequence
T5091 3067-3076 VBG denotes searching
T5090 3063-3066 IN denotes for
T5089 3053-3057 VBD denotes were
T5088 3045-3051 NN denotes 3Dpssm
T5087 3041-3044 CC denotes and
T5086 3033-3039 NN denotes ExPASy
T5085 3031-3033 , denotes ,
T5084 3018-3023 NN denotes motif
T5083 3024-3030 NN denotes search
T5082 3009-3017 NN denotes sequence
T5081 3007-3009 , denotes ,
T5080 2995-3006 NN denotes ProfileScan
T5079 2993-2995 , denotes ,
T5078 2977-2983 NN denotes Search
T5077 2984-2992 NN denotes Launcher
T5076 2973-2976 NN denotes BCM
T5075 2963-2972 VBG denotes including
T5074 3058-3062 VBN denotes used
T5073 2954-2962 NNS denotes programs
T5072 2945-2953 JJ denotes Multiple
T5071 2944-3112 sentence denotes Multiple programs including BCM Search Launcher , ProfileScan , sequence motif search , ExPASy and 3Dpssm were used for searching sequence features of known protein.
T5070 2943-2944 . denotes .
T5069 2924-2932 NN denotes sequence
T5068 2915-2923 JJ denotes pairwise
T5067 2911-2914 CC denotes and
T5066 2933-2942 NN denotes alignment
T5065 2903-2910 NN denotes GeneDoc
T5064 2898-2902 IN denotes with
T5063 2883-2887 VBD denotes were
T5062 2888-2897 VBN denotes performed
T5061 2863-2871 NN denotes sequence
T5060 2872-2882 NNS denotes alignments
T5059 2854-2862 JJ denotes Multiple
T5058 2853-2944 sentence denotes Multiple sequence alignments were performed with GeneDoc and pairwise sequence alignment .
T5057 2852-2853 . denotes .
T5056 2836-2842 NN denotes blastn
T5055 2832-2835 CC denotes and
T5054 2825-2831 NN denotes blastp
T5053 2823-2825 , denotes ,
T5052 2816-2823 NN denotes tblastx
T5051 2814-2816 , denotes ,
T5050 2843-2851 NNS denotes programs
T5049 2807-2814 NN denotes tblastn
T5048 2802-2806 IN denotes with
T5047 2798-2801 RP denotes out
T5046 2785-2789 VBD denotes were
T5045 2790-2797 VBN denotes carried
T5044 2776-2784 NNS denotes searches
T5043 2763-2766 NN denotes DNA
T5042 2759-2762 CC denotes and
T5041 2767-2775 NN denotes homology
T5040 2751-2758 NN denotes Protein
T5039 2750-2853 sentence denotes Protein and DNA homology searches were carried out with tblastn, tblastx, blastp and blastn programs .
T5038 2749-2750 . denotes .
T5037 2748-2749 -RRB- denotes )
T5036 2738-2743 NNPS denotes Codes
T5035 2733-2737 NNP denotes Gene
T5034 2744-2748 NNP denotes Corp
T5033 2732-2733 -LRB- denotes (
T5032 2721-2731 NN denotes Sequencher
T5031 2713-2720 NN denotes program
T5030 2708-2712 IN denotes with
T5029 2694-2697 VBD denotes was
T5028 2698-2707 VBN denotes performed
T5027 2685-2693 NN denotes assembly
T5026 2676-2684 NN denotes Sequence
T5025 2675-2750 sentence denotes Sequence assembly was performed with program Sequencher (Gene Codes Corp).
T5024 2667-2675 NNS denotes analyses
T5023 2658-2666 NN denotes Sequence
T5004 2655-2656 . denotes .
T5003 2647-2655 NN denotes analysis
T5002 2643-2646 IN denotes for
T5001 2617-2626 NNP denotes Radiation
T5000 2627-2633 NNP denotes Hybrid
T4999 2611-2616 NNP denotes Mouse
T4998 2600-2610 NNP denotes Laboratory
T4997 2592-2599 NNP denotes Jackson
T4996 2634-2642 NNP denotes Database
T4995 2588-2591 DT denotes the
T4994 2585-2587 IN denotes to
T4993 2570-2574 VBD denotes were
T4992 2575-2584 VBN denotes submitted
T4991 2565-2569 NNS denotes data
T4990 2561-2564 DT denotes The
T4989 2560-2656 sentence denotes The data were submitted to the Jackson Laboratory Mouse Radiation Hybrid Database for analysis.
T4988 2559-2560 . denotes .
T4987 2547-2552 NN denotes mouse
T4986 2553-2559 NN denotes genome
T4985 2543-2546 DT denotes the
T4984 2530-2542 VBG denotes representing
T4983 2516-2522 JJ denotes hybrid
T4982 2506-2515 NN denotes radiation
T4981 2502-2505 CD denotes 100
T4980 2523-2529 NNS denotes clones
T4979 2498-2501 DT denotes the
T4978 2490-2497 VB denotes amplify
T4977 2487-2489 TO denotes to
T4976 2477-2481 VBD denotes were
T4975 2465-2469 NN denotes Acdp
T4974 2470-2476 NN denotes member
T4973 2460-2464 DT denotes each
T4972 2457-2459 IN denotes of
T5655 5232-5233 . denotes .
T5654 5231-5232 -RRB- denotes )
T5653 5229-5231 NNP denotes MD
T5652 5227-5229 , denotes ,
T5651 5218-5227 NNP denotes Frederick
T5650 5216-5218 , denotes ,
T5649 5199-5204 NNP denotes Scion
T5648 5205-5216 NNP denotes Corporation
T5647 5198-5199 -LRB- denotes (
T5646 5183-5188 NN denotes Image
T5645 5177-5182 NNP denotes Scion
T5644 5189-5197 NN denotes software
T5643 5173-5176 DT denotes the
T5642 5170-5172 IN denotes by
T5641 5161-5169 VBN denotes acquired
T5640 5157-5160 CC denotes and
T5639 5146-5149 NN denotes CCD
T5638 5150-5156 NN denotes camera
T5637 5144-5145 DT denotes a
T5636 5139-5143 IN denotes with
T5635 5125-5129 VBD denotes were
T5634 5130-5138 VBN denotes captured
T5633 5118-5124 NNS denotes Images
T5632 5117-5233 sentence denotes Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5631 5116-5117 . denotes .
T5630 5115-5116 -RRB- denotes )
T5629 5105-5109 NNP denotes Carl
T5628 5110-5115 NNP denotes Zeiss
T5627 5104-5105 -LRB- denotes (
T5560 4766-4768 DT denotes an
T5559 4761-4765 IN denotes with
T5558 4746-4750 VBD denotes were
T5557 4740-4745 NNS denotes cells
T5556 4736-4739 DT denotes the
T5555 4734-4736 , denotes ,
T5554 4722-4725 NN denotes PBS
T5553 4726-4734 NN denotes solution
T5552 4711-4715 NN denotes goat
T5551 4716-4721 NN denotes serum
T5550 4709-4710 NN denotes %
T5549 4708-4709 CD denotes 1
T5548 4703-4707 IN denotes with
T5547 4695-4702 NN denotes washing
T5546 4685-4694 JJ denotes extensive
T5545 4751-4760 VBN denotes incubated
T5544 4679-4684 IN denotes After
T5543 4678-4894 sentence denotes After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5542 4677-4678 . denotes .
T5541 4675-4677 NN denotes °C
T5540 4674-4675 CD denotes 4
T5539 4671-4673 IN denotes at
T5538 4661-4670 RB denotes overnight
T5537 4659-4660 -RRB- denotes )
T5536 4655-4659 CD denotes 3000
T5535 4654-4655 SYM denotes :
T5534 4653-4654 CD denotes 1
T5533 4652-4653 -LRB- denotes (
T5532 4633-4642 JJ denotes anti-ACDP
T5531 4622-4632 JJ denotes polyclonal
T5530 4615-4621 NN denotes rabbit
T5529 4643-4651 NN denotes antibody
T5528 4613-4614 DT denotes a
T5527 4608-4612 IN denotes with
T5526 4598-4607 VBN denotes incubated
T5525 4585-4597 RB denotes subsequently
T5524 4581-4584 CC denotes and
T5523 4579-4581 , denotes ,
T5522 4569-4573 NN denotes goat
T5521 4562-4568 JJ denotes normal
T5520 4559-4561 CD denotes 30
T5519 4558-4559 SYM denotes :
T5518 4574-4579 NN denotes serum
T5517 4557-4558 CD denotes 1
T5516 4546-4556 VBG denotes containing
T5515 4528-4536 VBG denotes blocking
T5514 4537-4545 NN denotes solution
T5513 4526-4527 DT denotes a
T5512 4521-4525 IN denotes with
T5511 4506-4510 VBD denotes were
T5510 4500-4505 NNS denotes cells
T5509 4496-4499 DT denotes the
T5508 4494-4496 , denotes ,
T5507 4489-4494 NNS denotes times
T5506 4483-4488 CD denotes three
T5505 4479-4482 NN denotes PBS
T5504 4474-4478 IN denotes with
T5503 4466-4473 VBG denotes washing
T5502 4511-4520 VBN denotes incubated
T5501 4460-4465 IN denotes After
T5500 4459-4678 sentence denotes After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5499 4458-4459 . denotes .
T5498 4456-4458 NN denotes hr
T5497 4454-4455 CD denotes 1
T5496 4450-4453 IN denotes for
T5495 4447-4449 NN denotes °C
T5494 4446-4447 CD denotes 4
T5493 4443-4445 IN denotes at
T5492 4438-4439 HYPH denotes -
T5491 4437-4438 NN denotes X
T5490 4430-4436 NN denotes Triton
T5489 4439-4442 NN denotes 100
T5488 4428-4429 NN denotes %
T5487 4425-4428 CD denotes 0.4
T5486 4421-4424 CC denotes and
T5485 4417-4420 NN denotes PFA
T5484 4415-4416 NN denotes %
T5483 4414-4415 CD denotes 4
T5482 4403-4413 VBG denotes containing
T5481 4394-4402 NN denotes solution
T5480 4392-4393 DT denotes a
T5479 4389-4391 IN denotes in
T5478 4379-4388 VBN denotes incubated
T5477 4374-4378 RB denotes then
T5476 4370-4373 CC denotes and
T5475 4367-4369 NN denotes °C
T5474 4366-4367 CD denotes 4
T5473 4363-4365 IN denotes at
T5472 4359-4362 NNS denotes hrs
T5471 4356-4358 CD denotes 12
T5470 4352-4355 IN denotes for
T5469 4350-4351 -RRB- denotes )
T5468 4347-4350 NN denotes PFA
T5467 4346-4347 -LRB- denotes (
T5466 4329-4345 NN denotes paraformaldehyde
T5465 4327-4328 NN denotes %
T5464 4326-4327 CD denotes 4
T5463 4315-4325 VBG denotes containing
T5462 4311-4314 NN denotes PBS
T5461 4308-4310 IN denotes in
T5460 4296-4301 RB denotes first
T5459 4291-4295 VBD denotes were
T5458 4280-4290 NNS denotes coverslips
T5457 4276-4279 DT denotes the
T5456 4273-4275 IN denotes on
T5455 4267-4272 NNS denotes cells
T5454 4258-4266 JJ denotes neuronal
T5453 4256-4258 , denotes ,
T5452 4242-4256 NN denotes immunostaining
T5451 4302-4307 VBN denotes fixed
T5450 4238-4241 IN denotes For
T5449 4237-4459 sentence denotes For immunostaining, neuronal cells on the coverslips were first fixed in PBS containing 4% paraformaldehyde (PFA) for 12 hrs at 4°C and then incubated in a solution containing 4% PFA and 0.4% Triton X-100 at 4°C for 1 hr.
T5448 4236-4237 . denotes .
T5447 4226-4236 NNS denotes coverslips
T5446 4218-4225 VBG denotes coating
T5445 4214-4217 IN denotes for
T5444 4204-4208 VBD denotes were
T5443 4198-4203 NNS denotes cells
T5442 4194-4197 DT denotes the
T5441 4192-4194 , denotes ,
T5440 4186-4192 NN denotes debris
T5439 4177-4185 JJ denotes cellular
T5438 4172-4176 IN denotes with
T5437 4166-4171 NNS denotes media
T5436 4162-4165 DT denotes the
T5435 4159-4161 IN denotes of
T5434 4152-4158 NN denotes remove
T5433 4209-4213 VBN denotes used
T5432 4146-4151 IN denotes After
T5431 4145-4237 sentence denotes After remove of the media with cellular debris, the cells were used for coating coverslips.
T5430 4144-4145 . denotes .
T5429 4142-4144 NN denotes °C
T5428 4140-4142 CD denotes 37
T5427 4137-4139 IN denotes at
T5426 4129-4130 SYM denotes –
T5425 4130-4132 CD denotes 24
T5424 4127-4129 CD denotes 12
T5423 4133-4136 NNS denotes hrs
T5422 4116-4126 JJ denotes additional
T5421 4112-4115 IN denotes for
T5420 4103-4111 VBN denotes cultured
T5419 4099-4102 CC denotes and
T5418 4097-4098 -RRB- denotes )
T5417 4092-4097 NNP denotes Sigma
T5416 4091-4092 -LRB- denotes (
T5415 4070-4078 NN denotes cytosine
T5414 4079-4090 NN denotes arabinoside
T5413 4068-4069 NN denotes M
T5412 4065-4066 SYM denotes -
T5411 4066-4067 CD denotes 5
T5410 4063-4065 CD denotes 10
T5409 4060-4062 IN denotes to
T5408 4047-4051 VBD denotes were
T5407 4052-4059 VBN denotes exposed
T5406 4041-4046 NNS denotes cells
T5405 4037-4040 DT denotes The
T5404 4036-4145 sentence denotes The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C.
T5403 4035-4036 . denotes .
T5402 4026-4035 JJ denotes confluent
T5401 4020-4025 IN denotes until
T5400 4016-4019 NN denotes CO2
T5399 4014-4015 NN denotes %
T5398 4013-4014 CD denotes 5
T5397 4008-4012 IN denotes with
T5396 3986-3996 JJ denotes humidified
T5395 3997-4007 NN denotes atmosphere
T5394 3984-3985 DT denotes a
T5393 3981-3983 IN denotes in
T5392 3978-3980 NN denotes °C
T5391 3976-3978 CD denotes 37
T5390 3973-3975 IN denotes at
T5389 3966-3972 NNS denotes flasks
T5388 3958-3965 NN denotes culture
T5387 3955-3957 IN denotes in
T5386 3944-3948 VBD denotes were
T5385 3935-3943 NNS denotes cortices
T5384 3929-3934 NN denotes mouse
T5383 3921-3928 JJ denotes newborn
T5382 3916-3920 IN denotes from
T5381 3904-3915 VBN denotes dissociated
T5380 3949-3954 VBN denotes grown
T5379 3893-3903 NNS denotes astrocytes
T5378 3884-3892 JJ denotes Cortical
T5377 3883-4036 sentence denotes Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent.
T5376 3882-3883 . denotes .
T5375 3879-3882 NN denotes CO2
T5374 3877-3878 NN denotes %
T5373 3876-3877 CD denotes 5
T5372 3871-3875 IN denotes with
T5371 3849-3859 JJ denotes humidified
T4971 2451-2452 SYM denotes '
T4970 2453-2456 NN denotes UTR
T4969 2450-2451 CD denotes 3
T4968 2445-2449 IN denotes from
T4967 2482-2486 VBN denotes used
T4966 2437-2444 NNS denotes Primers
T4965 2436-2560 sentence denotes Primers from 3' UTR of each Acdp member were used to amplify the 100 radiation hybrid clones representing the mouse genome.
T4964 2435-2436 . denotes .
T4963 2424-2428 NN denotes Acdp
T4962 2429-2435 NN denotes member
T4961 2419-2423 DT denotes each
T4960 2416-2418 IN denotes of
T4959 2396-2406 NN denotes chromosome
T4958 2407-2415 NN denotes location
T4957 2392-2395 DT denotes the
T4956 2388-2391 VB denotes map
T4955 2385-2387 TO denotes to
T4954 2376-2379 VBD denotes was
T4953 2367-2375 NNP denotes Genetics
T4952 2358-2366 NNP denotes Research
T4951 2353-2357 IN denotes from
T4950 2380-2384 VBN denotes used
T4949 2340-2346 NN denotes hybrid
T4948 2330-2339 NN denotes radiation
T4947 2324-2329 NN denotes mouse
T4946 2320-2323 NN denotes T31
T4945 2347-2352 NN denotes panel
T4944 2316-2319 DT denotes The
T4943 2315-2436 sentence denotes The T31 mouse radiation hybrid panel from Research Genetics was used to map the chromosome location of each Acdp member.
T4942 2303-2315 NN denotes localization
T4941 2292-2302 NN denotes Chromosome
T4917 2289-2290 . denotes .
T4916 2276-2284 NN denotes exposure
T4915 2285-2289 NN denotes time
T4914 2268-2275 JJ denotes optimal
T4913 2263-2267 IN denotes with
T4912 2257-2262 NNS denotes films
T4911 2251-2256 NN denotes X-ray
T4910 2246-2250 IN denotes with
T4909 2231-2235 RB denotes then
T4908 2236-2245 VBN denotes developed
T4907 2226-2230 VBD denotes were
T4906 2222-2225 CC denotes and
T4905 2201-2210 JJ denotes secondary
T4904 2197-2200 CC denotes and
T4903 2211-2221 NNS denotes antibodies
T4902 2189-2196 JJ denotes primary
T4901 2184-2188 IN denotes with
T4900 2173-2183 NN denotes incubation
T4899 2167-2172 IN denotes after
T4898 2155-2166 RB denotes extensively
T4897 2143-2147 VBD denotes were
T4896 2148-2154 VBN denotes washed
T4895 2133-2142 NNS denotes membranes
T4894 2129-2132 DT denotes The
T4893 2128-2290 sentence denotes The membranes were washed extensively after incubation with primary and secondary antibodies and were then developed with X-ray films with optimal exposure time.
T4892 2127-2128 . denotes .
T4891 2126-2127 -RRB- denotes ]
T4890 2125-2126 CD denotes 1
T4889 2124-2125 -LRB- denotes [
T4888 2113-2123 RB denotes previously
T4887 2103-2112 VBN denotes described
T4886 2100-2102 IN denotes as
T4885 2098-2099 -RRB- denotes )
T4786 1775-1776 . denotes .
T4785 1774-1775 -RRB- denotes ]
T4784 1772-1773 , denotes ,
T4783 1771-1772 CD denotes 3
T4782 1773-1774 CD denotes 4
T4781 1770-1771 -LRB- denotes [
T4780 1763-1769 RBR denotes longer
T4779 1760-1762 CC denotes or
T4778 1750-1759 NN denotes overnight
T4777 1746-1749 IN denotes for
T4776 1740-1745 NNS denotes films
T4775 1734-1739 NN denotes X-ray
T4774 1731-1733 IN denotes to
T4773 1718-1722 VBD denotes were
T4772 1723-1730 VBN denotes exposed
T4771 1710-1717 NNS denotes filters
T4770 1703-1709 JJ denotes Washed
T4769 1702-1776 sentence denotes Washed filters were exposed to X-ray films for overnight or longer [3,4].
T4768 1701-1702 . denotes .
T4767 1697-1698 HYPH denotes -
T4766 1698-1701 NN denotes min
T4765 1695-1697 CD denotes 15
T4764 1691-1694 IN denotes for
T4763 1688-1690 NN denotes °C
T4762 1686-1688 CD denotes 65
T4761 1683-1685 IN denotes at
T4760 1681-1682 -RRB- denotes )
T4759 1676-1677 NN denotes %
T4758 1673-1676 CD denotes 0.1
T4757 1671-1673 , denotes ,
T4756 1667-1668 SYM denotes ×
T4755 1668-1671 NN denotes SSC
T4754 1663-1667 CD denotes 0.25
T4753 1678-1681 NN denotes SDS
T4752 1662-1663 -LRB- denotes (
T4751 1659-1661 CD denotes II
T4750 1652-1658 NN denotes buffer
T4749 1644-1651 NN denotes washing
T4748 1639-1643 IN denotes with
T4747 1633-1638 RB denotes twice
T4746 1626-1632 VBD denotes washed
T4745 1621-1625 RB denotes then
T4744 1617-1620 CC denotes and
T4743 1615-1617 , denotes ,
T4742 1611-1612 HYPH denotes -
T4741 1612-1615 NN denotes min
T4740 1609-1611 CD denotes 15
T4739 1605-1608 IN denotes for
T4738 1602-1604 NN denotes °C
T4737 1600-1602 CD denotes 42
T4736 1597-1599 IN denotes at
T4735 1595-1596 -RRB- denotes )
T4734 1590-1591 NN denotes %
T4733 1587-1590 CD denotes 0.1
T4732 1585-1587 , denotes ,
T4731 1581-1582 SYM denotes ×
T4730 1582-1585 NN denotes SSC
T4729 1580-1581 CD denotes 2
T4728 1592-1595 NN denotes SDS
T4727 1579-1580 -LRB- denotes (
T4726 1577-1578 CD denotes I
T4725 1570-1576 NN denotes buffer
T4724 1562-1569 VBG denotes washing
T4723 1557-1561 IN denotes with
T4722 1551-1556 RB denotes twice
T4721 1539-1543 VBD denotes were
T4720 1544-1550 VBN denotes washed
T4719 1531-1538 NNS denotes filters
T4718 1527-1530 DT denotes The
T4717 1526-1702 sentence denotes The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min.
T4716 1525-1526 . denotes .
T4715 1516-1525 RB denotes overnight
T4714 1512-1515 RP denotes out
T4713 1499-1503 VBD denotes were
T4712 1504-1511 VBN denotes carried
T4711 1484-1498 NNS denotes Hybridizations
T4710 1483-1526 sentence denotes Hybridizations were carried out overnight.
T4709 1482-1483 . denotes .
T4708 1481-1482 -RRB- denotes )
T4707 1464-1468 NNP denotes Life
T4706 1469-1481 NNP denotes Technologies
T4705 1463-1464 -LRB- denotes (
T4704 1446-1455 NN denotes extension
T4703 1439-1445 NN denotes primer
T4702 1432-1438 JJ denotes random
T4701 1456-1462 NN denotes system
T4700 1428-1431 DT denotes the
T4699 1422-1427 VBG denotes using
T4698 1417-1421 NN denotes dCTP
T4697 1412-1413 HYPH denotes -
T4696 1413-1416 NN denotes 32P
T4695 1411-1412 NN denotes α
T4694 1406-1410 IN denotes with
T4693 1398-1405 VBN denotes labeled
T4692 1378-1390 JJ denotes untranslated
T4691 1376-1377 SYM denotes '
T4690 1391-1397 NN denotes region
T4689 1375-1376 CD denotes 3
T4688 1371-1374 DT denotes the
T4687 1367-1370 CC denotes and
T4686 1362-1366 NN denotes exon
T5626 5084-5092 JJ denotes confocal
T5625 5093-5103 NN denotes microscope
T5624 5082-5083 DT denotes a
T5623 5076-5081 IN denotes under
T5622 5064-5068 VBD denotes were
T5621 5069-5075 VBN denotes viewed
T5620 5058-5063 NNS denotes cells
T5619 5054-5057 DT denotes The
T5618 5053-5117 sentence denotes The cells were viewed under a confocal microscope (Carl Zeiss).
T5617 5052-5053 . denotes .
T5616 5029-5045 JJ denotes anti-photobleach
T5615 5022-5023 HYPH denotes -
T5614 5023-5028 VBN denotes based
T5613 5014-5022 NN denotes glycerol
T5612 5046-5052 NN denotes medium
T5611 5012-5013 DT denotes a
T5610 5007-5011 IN denotes with
T5609 4998-4999 HYPH denotes -
T5608 4993-4998 NN denotes cover
T5607 4988-4992 VBD denotes were
T5606 4977-4987 NNS denotes coverslips
T5605 4973-4976 DT denotes the
T5604 4970-4972 IN denotes on
T5603 4955-4963 JJ denotes neuronal
T5602 4964-4969 NNS denotes cells
T5601 4951-4954 DT denotes the
T5600 4949-4951 , denotes ,
T5599 4937-4940 NN denotes PBS
T5598 4941-4949 NN denotes solution
T5597 4926-4930 NN denotes goat
T5596 4931-4936 NN denotes serum
T5595 4924-4925 NN denotes %
T5594 4923-4924 CD denotes 1
T5593 4918-4922 IN denotes with
T5592 4911-4917 NNS denotes washes
T5591 4905-4910 JJ denotes final
T5590 4999-5006 VBN denotes slipped
T5589 4895-4904 IN denotes Following
T5588 4894-5053 sentence denotes Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5587 4893-4894 . denotes .
T5586 4882-4893 NN denotes temperature
T5585 4877-4881 NN denotes room
T5584 4874-4876 IN denotes at
T5583 4870-4873 NNS denotes hrs
T5582 4868-4869 CD denotes 3
T5581 4864-4867 IN denotes for
T5580 4862-4863 -RRB- denotes )
T5579 4846-4855 NNP denotes Molecular
T5578 4844-4846 , denotes ,
T5577 4832-4835 NN denotes PBS
T5576 4836-4844 NN denotes solution
T5575 4821-4825 NN denotes goat
T5574 4826-4831 NN denotes serum
T5573 4819-4820 NN denotes %
T5572 4818-4819 CD denotes 1
T5571 4815-4817 IN denotes in
T5570 4811-4814 CD denotes 100
T5569 4810-4811 SYM denotes :
T5568 4809-4810 CD denotes 1
T5567 4856-4862 NNP denotes Probes
T5566 4808-4809 -LRB- denotes (
T5565 4789-4798 JJ denotes secondary
T5564 4778-4788 JJ denotes conjugated
T5563 4774-4777 CD denotes 488
T5562 4769-4773 NNP denotes Alex
T5561 4799-4807 NN denotes antibody
T5125 3265-3266 . denotes .
T5124 3264-3265 -RRB- denotes ]
T5123 3262-3264 CD denotes 14
T5122 3261-3262 -LRB- denotes [
T5121 3249-3250 -RRB- denotes )
T5120 3241-3249 NNS denotes averages
T5119 3230-3240 NN denotes Arithmetic
T5118 3224-3229 VBG denotes using
T5117 3211-3216 NN denotes Group
T5116 3206-3210 NN denotes Pair
T5115 3217-3223 NN denotes Method
T5114 3195-3205 JJ denotes Unweighted
T5113 3194-3195 -LRB- denotes (
T5112 3251-3260 NN denotes algorithm
T5111 3188-3193 NN denotes UPGMA
T5110 3182-3187 VBG denotes using
T5109 3180-3181 -RRB- denotes )
T5108 3176-3180 CD denotes 1.81
T5107 3168-3175 NN denotes version
T5106 3167-3168 -LRB- denotes (
T5105 3159-3166 NN denotes program
T5104 3150-3158 NN denotes Clustalw
T5103 3147-3149 IN denotes by
T5102 3131-3134 VBD denotes was
T5101 3135-3146 VBN denotes constructed
T4685 1357-1361 JJ denotes last
T4684 1352-1356 IN denotes from
T4683 1350-1351 -RRB- denotes )
T4682 1344-1347 CD denotes 350
T4681 1337-1343 IN denotes around
T4680 1348-1350 NN denotes bp
T4679 1336-1337 -LRB- denotes (
T4678 1323-1326 NN denotes PCR
T4677 1327-1335 NN denotes fragment
T4676 1321-1322 DT denotes a
T4675 1316-1320 IN denotes with
T4674 1306-1310 NN denotes Acdp
T4673 1311-1315 NN denotes gene
T4672 1301-1305 DT denotes each
T4671 1297-1300 IN denotes for
T4670 1285-1289 VBD denotes were
T4669 1290-1296 VBN denotes probed
T4668 1277-1284 NNS denotes filters
T4667 1273-1276 DT denotes The
T4666 1272-1483 sentence denotes The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies).
T4665 1271-1272 . denotes .
T4664 1264-1271 NNP denotes Origene
T4663 1259-1263 IN denotes from
T4662 1244-1248 VBD denotes were
T4661 1230-1235 NN denotes mouse
T4660 1220-1229 JJ denotes different
T4659 1236-1243 NNS denotes tissues
T4658 1217-1219 CD denotes 12
T4657 1206-1216 VBG denotes containing
T4656 1249-1258 VBN denotes purchased
T4655 1193-1197 NN denotes Blot
T4654 1184-1192 NNP denotes Northern
T4653 1177-1183 NN denotes Choice
T4652 1198-1205 NNS denotes filters
T4651 1168-1176 JJ denotes Multiple
T4650 1167-1272 sentence denotes Multiple Choice Northern Blot filters containing 12 different mouse tissues were purchased from Origene.
T4649 1159-1167 NNS denotes analyses
T4648 1154-1158 NN denotes blot
T4647 1145-1153 NNP denotes Northern
T4607 1142-1143 . denotes .
T4606 1128-1131 NN denotes DNA
T4605 1132-1142 NN denotes sequencing
T4604 1124-1127 NN denotes BAC
T4603 1121-1123 IN denotes by
T4602 1107-1111 VBD denotes were
T4601 1091-1092 SYM denotes '
T4600 1090-1091 CD denotes 5
T4599 1093-1096 NN denotes UTR
T4587 1021-1027 VBG denotes coding
T4586 1028-1037 NNS denotes sequences
T4585 1017-1020 DT denotes the
T4584 1014-1016 IN denotes of
T4583 1009-1013 JJS denotes most
T4582 1043-1053 VBN denotes identified
T4581 1004-1008 IN denotes Once
T4580 1003-1143 sentence denotes Once most of the coding sequences were identified, partial sequence of exon 1 and the 5' UTR sequences were obtained by BAC DNA sequencing.
T4579 1002-1003 . denotes .
T4578 994-1002 NN denotes sequence
T4577 988-993 JJ denotes known
T4576 983-987 IN denotes from
T4575 968-975 JJ denotes reverse
T4574 976-982 NN denotes primer
T4573 966-967 DT denotes a
T4572 962-965 CC denotes and
T4571 953-961 NN denotes sequence
T4570 942-952 VBN denotes identified
T4569 936-941 RB denotes newly
T4568 931-935 IN denotes from
T4567 916-923 JJ denotes forward
T4566 924-930 NN denotes primer
T4565 914-915 DT denotes a
T4564 908-913 VBG denotes using
T4563 890-897 RB denotes further
T4562 886-889 VBD denotes was
T4561 898-907 VBN denotes confirmed
T4560 877-885 NN denotes sequence
T4559 873-876 DT denotes The
T4558 872-1003 sentence denotes The sequence was further confirmed using a forward primer from newly identified sequence and a reverse primer from known sequence.
T4557 871-872 . denotes .
T4556 852-861 JJ denotes automatic
T4555 845-851 NN denotes ABI377
T4554 862-871 NN denotes sequencer
T4553 842-844 DT denotes an
T4552 839-841 IN denotes by
T4551 829-838 VBN denotes sequenced
T4550 825-828 CC denotes and
T4549 821-824 NN denotes gel
T4548 813-820 NN denotes agarose
T4547 808-812 IN denotes from
T4546 791-799 RB denotes directly
T4545 786-790 VBD denotes were
T4544 800-807 VBN denotes excised
T4543 773-776 NN denotes PCR
T4542 764-772 JJ denotes expected
T4541 777-785 NNS denotes products
T4540 760-763 DT denotes The
T4539 759-872 sentence denotes The expected PCR products were directly excised from agarose gel and sequenced by an ABI377 automatic sequencer.
T4538 758-759 . denotes .
T4537 757-758 -RRB- denotes )
T4536 755-757 NN denotes °C
T4535 753-755 CD denotes 62
T4534 752-753 -LRB- denotes (
T4533 730-739 VBG denotes annealing
T4532 740-751 NN denotes temperature
T4531 725-729 JJ denotes high
T4530 722-724 IN denotes at
T4529 709-712 NN denotes PCR
T4528 703-708 NN denotes round
T4527 697-702 JJ denotes first
T4526 713-721 NNS denotes products
T4525 693-696 DT denotes the
T4524 688-692 IN denotes from
T4523 677-682 NN denotes mouse
T4522 668-676 JJ denotes specific
T4521 683-687 NN denotes gene
T4520 664-667 DT denotes the
T4519 656-663 VB denotes amplify
T4518 653-655 TO denotes to
T4517 649-652 RP denotes out
T4516 636-640 RB denotes then
T4515 632-635 VBD denotes was
T4514 617-624 JJ denotes forward
T4513 611-616 JJ denotes human
T4512 606-610 JJ denotes same
T4511 625-631 NN denotes primer
T4510 602-605 DT denotes the
T4509 598-601 CC denotes and
T4508 585-588 NN denotes EST
T4507 579-584 NN denotes mouse
T4506 589-597 NN denotes sequence
T4505 575-578 DT denotes the
T4504 570-574 IN denotes from
T4503 555-562 JJ denotes reverse
T4502 548-554 JJ denotes inside
T4501 563-569 NN denotes primer
T4500 545-547 DT denotes an
T4499 539-544 VBG denotes using
T4498 641-648 VBN denotes carried
T4497 528-534 JJ denotes nested
T4496 535-538 NN denotes PCR
T4495 526-527 DT denotes A
T4494 525-759 sentence denotes A nested PCR using an inside reverse primer from the mouse EST sequence and the same human forward primer was then carried out to amplify the specific mouse gene from the first round PCR products at high annealing temperature (62°C).
T4493 524-525 . denotes .
T4492 523-524 -RRB- denotes )
T4491 521-523 NN denotes °C
T4490 518-519 SYM denotes –
T4489 519-521 CD denotes 50
T4488 516-518 CD denotes 45
T4487 515-516 -LRB- denotes (
T4486 493-502 VBG denotes annealing
T4485 503-514 NN denotes temperature
T4484 489-492 JJ denotes low
T4483 484-488 RB denotes very
T4482 481-483 IN denotes at
T4481 476-480 NN denotes cDNA
T4480 470-475 NN denotes mouse
T4479 465-469 IN denotes from
T4478 446-455 NN denotes homologue
T4477 456-464 NN denotes sequence
T4476 442-445 DT denotes the
T4475 434-441 VB denotes amplify
T4474 431-433 TO denotes to
T4473 421-425 VBD denotes were
T4472 410-413 NN denotes EST
T4471 404-409 NN denotes mouse
T4470 414-420 NN denotes marker
T4469 400-403 DT denotes the
T4468 395-399 IN denotes from
T4467 380-387 JJ denotes reverse
T4466 374-379 NN denotes mouse
T4465 388-394 NN denotes primer
T4464 372-373 DT denotes a
T4463 368-371 CC denotes and
T4462 366-367 -RRB- denotes )
T4461 361-366 NN denotes codon
T4460 355-360 NN denotes start
T4459 349-354 IN denotes after
T4884 2092-2098 NN denotes PIERCE
T4883 2091-2092 -LRB- denotes (
T4882 2087-2090 NN denotes ECL
T4881 2081-2086 VBG denotes Using
T4880 2077-2080 RP denotes out
T4879 2064-2068 VBD denotes were
T4878 2069-2076 VBN denotes carried
T4877 2058-2063 NNS denotes blots
T4876 2050-2057 NNP denotes Western
T4875 2049-2128 sentence denotes Western blots were carried out Using ECL (PIERCE) as described previously [1].
T4874 2048-2049 . denotes .
T4873 2047-2048 -RRB- denotes ]
T4872 2046-2047 CD denotes 5
T4871 2045-2046 -LRB- denotes [
T4870 2032-2044 RB denotes respectively
T4869 2030-2032 , denotes ,
T4868 2022-2030 VBN denotes reported
T4867 2019-2021 IN denotes as
T4866 2006-2010 NN denotes Acdp
T4865 2011-2018 NNS denotes members
T4864 2002-2005 DT denotes all
T4863 1998-2001 CC denotes and
T4862 1992-1997 NN denotes Acdp1
T4861 1988-1991 IN denotes for
T4860 1975-1987 RB denotes specifically
T4859 1964-1974 NNS denotes antibodies
T4858 1961-1963 IN denotes of
T4857 1950-1960 NN denotes generation
T4856 1946-1949 IN denotes for
T4855 1936-1940 VBD denotes were
T4854 1934-1935 -RRB- denotes )
T4853 1931-1934 NN denotes ACD
T4852 1930-1931 -LRB- denotes (
T4851 1913-1922 VBN denotes conserved
T4850 1923-1929 NN denotes domain
T4849 1909-1912 DT denotes the
T4848 1905-1908 CC denotes and
T4847 1894-1895 HYPH denotes -
T4846 1893-1894 NN denotes C
T4845 1889-1892 CC denotes and
T4844 1887-1888 HYPH denotes -
T4843 1886-1887 NN denotes N
T4842 1895-1904 NNS denotes terminals
T4841 1880-1885 NN denotes Acdp1
T4840 1875-1879 IN denotes from
T4839 1873-1874 -RRB- denotes )
T4838 1856-1862 NN denotes limpet
T4837 1863-1873 NN denotes hemacyanin
T4836 1848-1855 NN denotes keyhole
T4835 1847-1848 -LRB- denotes (
T4834 1843-1846 NN denotes KLH
T4833 1840-1842 IN denotes to
T4832 1833-1839 VBN denotes linked
T4831 1941-1945 VBN denotes used
T4830 1824-1832 NNS denotes Peptides
T4829 1823-2049 sentence denotes Peptides linked to KLH (keyhole limpet hemacyanin) from Acdp1 N- and C-terminals and the conserved domain (ACD) were used for generation of antibodies specifically for Acdp1 and all Acdp members as reported, respectively [5].
T4828 1815-1823 NNS denotes analyses
T4827 1810-1814 NN denotes blot
T4826 1802-1809 NNP denotes Western
T4825 1798-1801 CC denotes and
T4824 1787-1797 NN denotes production
T4823 1778-1786 NN denotes Antibody
T4458 348-349 -LRB- denotes (
T4457 334-340 NN denotes coding
T4456 329-333 NN denotes cDNA
T4455 327-328 SYM denotes '
T4454 326-327 CD denotes 5
T4453 321-325 NN denotes ACDP
T4452 315-320 JJ denotes human
T4451 341-347 NN denotes region
T4450 311-314 DT denotes the
T4449 304-310 IN denotes within
T4448 426-430 VBN denotes used
T4447 289-296 JJ denotes forward
T4446 297-303 NN denotes primer
T4445 287-288 DT denotes A
T4444 286-525 sentence denotes A forward primer within the human ACDP 5' cDNA coding region (after start codon) and a mouse reverse primer from the mouse EST marker were used to amplify the homologue sequence from mouse cDNA at very low annealing temperature (45–50°C).
T4443 285-286 . denotes .
T4442 274-278 NN denotes Acdp
T4441 279-285 NN denotes member
T4440 269-273 DT denotes each
T4439 266-268 IN denotes to
T4438 252-265 VBG denotes corresponding
T4437 244-251 NNS denotes markers
T4436 240-243 NN denotes EST
T4435 236-239 IN denotes for
T4434 223-226 NN denotes EST
T4433 217-222 NN denotes mouse
T4432 227-235 NN denotes database
T4431 213-216 DT denotes the
T4430 206-212 VB denotes search
T4429 203-205 TO denotes to
T4428 193-197 VBD denotes were
T4427 180-182 NN denotes AA
T4426 183-192 NNS denotes sequences
T4425 170-179 VBN denotes predicted
T4424 166-169 CC denotes and
T4423 155-159 NN denotes ACDP
T4422 149-154 JJ denotes human
T4421 160-165 NNS denotes cDNAs
T4420 145-148 DT denotes the
T4419 143-145 , denotes ,
T4418 198-202 VBN denotes used
T4417 136-143 RB denotes Briefly
T4416 135-286 sentence denotes Briefly, the human ACDP cDNAs and predicted AA sequences were used to search the mouse EST database for EST markers corresponding to each Acdp member.
T4415 134-135 . denotes .
T4414 133-134 -RRB- denotes ]
T4413 132-133 CD denotes 2
T4412 131-132 -LRB- denotes [
T4411 111-121 RB denotes previously
T4410 122-130 VBN denotes reported
T4409 108-110 IN denotes as
T4408 88-97 NN denotes homologue
T4407 98-107 NNS denotes sequences
T4406 82-87 JJ denotes human
T4405 79-81 IN denotes on
T4404 73-78 VBN denotes based
T4403 59-62 VBD denotes was
T4402 47-51 NN denotes gene
T4401 42-46 NN denotes Acdp
T4400 52-58 NN denotes family
T4399 38-41 DT denotes the
T4398 35-37 IN denotes of
T4397 63-72 VBN denotes performed
T4396 27-34 NN denotes cloning
T4395 22-26 NN denotes cDNA
T4394 21-135 sentence denotes cDNA cloning of the Acdp gene family was performed based on human homologue sequences as previously reported [2].
T4393 14-21 NN denotes cloning
T4392 9-13 NN denotes cDNA
T4598 1086-1089 DT denotes the
T4597 1082-1085 CC denotes and
T4596 1080-1081 CD denotes 1
T4595 1097-1106 NNS denotes sequences
T4594 1075-1079 NN denotes exon
T4593 1072-1074 IN denotes of
T4592 1063-1071 NN denotes sequence
T4591 1055-1062 JJ denotes partial
T4590 1053-1055 , denotes ,
T4589 1112-1120 VBN denotes obtained
T4588 1038-1042 VBD denotes were
T5719 5443-5444 . denotes .
T5679 5255-5261 NN denotes number
T5712 5416-5421 NN denotes Acdp3
T5711 5415-5416 -LRB- denotes (
T5710 5406-5414 NN denotes AF216964
T5709 5404-5406 , denotes ,
T5708 5403-5404 -RRB- denotes )
T5707 5398-5403 NN denotes Acdp2
T5706 5397-5398 -LRB- denotes (
T5705 5388-5396 NN denotes AF216961
T5704 5386-5388 , denotes ,
T5703 5385-5386 -RRB- denotes )
T5702 5380-5385 NN denotes Acdp1
R2573 T4392 T4393 compound cDNA,cloning
R2574 T4395 T4396 compound cDNA,cloning
R2575 T4396 T4397 nsubjpass cloning,performed
R2576 T4398 T4396 prep of,cloning
R2577 T4399 T4400 det the,family
R2578 T4400 T4398 pobj family,of
R2579 T4401 T4400 compound Acdp,family
R2580 T4402 T4400 compound gene,family
R2581 T4403 T4397 auxpass was,performed
R2582 T4404 T4397 prep based,performed
R2583 T4405 T4404 prep on,based
R2584 T4406 T4407 amod human,sequences
R2585 T4407 T4405 pobj sequences,on
R2586 T4408 T4407 compound homologue,sequences
R2587 T4409 T4410 mark as,reported
R2588 T4410 T4397 advcl reported,performed
R2589 T4411 T4410 advmod previously,reported
R2590 T4412 T4413 punct [,2
R2591 T4413 T4397 parataxis 2,performed
R2592 T4414 T4413 punct ],2
R2593 T4415 T4397 punct .,performed
R2594 T4417 T4418 advmod Briefly,used
R2595 T4419 T4418 punct ", ",used
R2596 T4420 T4421 det the,cDNAs
R2597 T4421 T4418 nsubjpass cDNAs,used
R2598 T4422 T4421 amod human,cDNAs
R2599 T4423 T4421 compound ACDP,cDNAs
R2600 T4424 T4421 cc and,cDNAs
R2601 T4425 T4426 amod predicted,sequences
R2602 T4426 T4421 conj sequences,cDNAs
R2603 T4427 T4426 compound AA,sequences
R2604 T4428 T4418 auxpass were,used
R2605 T4429 T4430 aux to,search
R2606 T4430 T4418 advcl search,used
R2607 T4431 T4432 det the,database
R2608 T4432 T4430 dobj database,search
R2609 T4433 T4432 compound mouse,database
R2610 T4434 T4432 compound EST,database
R2611 T4435 T4430 prep for,search
R2612 T4436 T4437 compound EST,markers
R2613 T4437 T4435 pobj markers,for
R2614 T4438 T4437 acl corresponding,markers
R2615 T4439 T4438 prep to,corresponding
R2616 T4440 T4441 det each,member
R2617 T4441 T4439 pobj member,to
R2618 T4442 T4441 compound Acdp,member
R2619 T4443 T4418 punct .,used
R2620 T4445 T4446 det A,primer
R2621 T4446 T4448 nsubjpass primer,used
R2622 T4447 T4446 amod forward,primer
R2623 T4449 T4446 prep within,primer
R2624 T4450 T4451 det the,region
R2625 T4451 T4449 pobj region,within
R2626 T4452 T4451 amod human,region
R2627 T4453 T4451 nmod ACDP,region
R2628 T4454 T4451 nummod 5,region
R2629 T4455 T4454 punct ',5
R2630 T4456 T4451 compound cDNA,region
R2631 T4457 T4451 compound coding,region
R2632 T4458 T4446 punct (,primer
R2633 T4459 T4446 prep after,primer
R2634 T4460 T4461 compound start,codon
R2635 T4461 T4459 pobj codon,after
R2636 T4462 T4446 punct ),primer
R2637 T4463 T4446 cc and,primer
R2638 T4464 T4465 det a,primer
R2639 T4465 T4446 conj primer,primer
R2640 T4466 T4465 nmod mouse,primer
R2641 T4467 T4465 amod reverse,primer
R2642 T4468 T4465 prep from,primer
R2643 T4469 T4470 det the,marker
R2644 T4470 T4468 pobj marker,from
R2645 T4471 T4470 compound mouse,marker
R2646 T4472 T4470 compound EST,marker
R2647 T4473 T4448 auxpass were,used
R2648 T4474 T4475 aux to,amplify
R2649 T4475 T4448 advcl amplify,used
R2650 T4476 T4477 det the,sequence
R2651 T4477 T4475 dobj sequence,amplify
R2652 T4478 T4477 compound homologue,sequence
R2653 T4479 T4475 prep from,amplify
R2654 T4480 T4481 compound mouse,cDNA
R2655 T4481 T4479 pobj cDNA,from
R2656 T4482 T4475 prep at,amplify
R2657 T4483 T4484 advmod very,low
R2658 T4484 T4485 amod low,temperature
R2659 T4485 T4482 pobj temperature,at
R2660 T4486 T4485 amod annealing,temperature
R2661 T4487 T4485 punct (,temperature
R2662 T4488 T4489 quantmod 45,50
R2663 T4489 T4491 nummod 50,°C
R2664 T4490 T4489 punct –,50
R2665 T4491 T4485 appos °C,temperature
R2666 T4492 T4448 punct ),used
R2667 T4493 T4448 punct .,used
R2668 T4495 T4496 det A,PCR
R2669 T4496 T4498 nsubjpass PCR,carried
R2670 T4497 T4496 amod nested,PCR
R2671 T4499 T4496 acl using,PCR
R2672 T4500 T4501 det an,primer
R2673 T4501 T4499 dobj primer,using
R2674 T4502 T4501 amod inside,primer
R2675 T4503 T4501 amod reverse,primer
R2676 T4504 T4501 prep from,primer
R2677 T4505 T4506 det the,sequence
R2678 T4506 T4504 pobj sequence,from
R2679 T4507 T4506 compound mouse,sequence
R2680 T4508 T4506 compound EST,sequence
R2681 T4509 T4501 cc and,primer
R2682 T4510 T4511 det the,primer
R2683 T4511 T4501 conj primer,primer
R2684 T4512 T4511 amod same,primer
R2685 T4513 T4511 amod human,primer
R2686 T4514 T4511 amod forward,primer
R2687 T4515 T4498 auxpass was,carried
R2688 T4516 T4498 advmod then,carried
R2689 T4517 T4498 prt out,carried
R2690 T4518 T4519 aux to,amplify
R2691 T4519 T4498 advcl amplify,carried
R2692 T4520 T4521 det the,gene
R2693 T4521 T4519 dobj gene,amplify
R2694 T4522 T4521 amod specific,gene
R2695 T4523 T4521 compound mouse,gene
R2696 T4524 T4521 prep from,gene
R2697 T4525 T4526 det the,products
R2698 T4526 T4524 pobj products,from
R2699 T4527 T4528 amod first,round
R2700 T4528 T4526 compound round,products
R2701 T4529 T4526 compound PCR,products
R2702 T4530 T4519 prep at,amplify
R2703 T4531 T4532 amod high,temperature
R2704 T4532 T4530 pobj temperature,at
R2705 T4533 T4532 amod annealing,temperature
R2706 T4534 T4532 punct (,temperature
R2707 T4535 T4536 nummod 62,°C
R2708 T4536 T4532 appos °C,temperature
R2709 T4537 T4498 punct ),carried
R2710 T4538 T4498 punct .,carried
R2711 T4540 T4541 det The,products
R2712 T4541 T4544 nsubjpass products,excised
R2713 T4542 T4541 amod expected,products
R2714 T4543 T4541 compound PCR,products
R2715 T4545 T4544 auxpass were,excised
R2716 T4546 T4544 advmod directly,excised
R2717 T4547 T4544 prep from,excised
R2718 T4548 T4549 compound agarose,gel
R2719 T4549 T4547 pobj gel,from
R2720 T4550 T4544 cc and,excised
R2721 T4551 T4544 conj sequenced,excised
R2722 T4552 T4551 prep by,sequenced
R2723 T4553 T4554 det an,sequencer
R2724 T4554 T4552 pobj sequencer,by
R2725 T4555 T4554 nmod ABI377,sequencer
R2726 T4556 T4554 amod automatic,sequencer
R2727 T4557 T4544 punct .,excised
R2728 T4559 T4560 det The,sequence
R2729 T4560 T4561 nsubjpass sequence,confirmed
R2730 T4562 T4561 auxpass was,confirmed
R2731 T4563 T4561 advmod further,confirmed
R2732 T4564 T4561 advcl using,confirmed
R2733 T4565 T4566 det a,primer
R2734 T4566 T4564 dobj primer,using
R2735 T4567 T4566 amod forward,primer
R2736 T4568 T4566 prep from,primer
R2737 T4569 T4570 advmod newly,identified
R2738 T4570 T4571 amod identified,sequence
R2739 T4571 T4568 pobj sequence,from
R2740 T4572 T4566 cc and,primer
R2741 T4573 T4574 det a,primer
R2742 T4574 T4566 conj primer,primer
R2743 T4575 T4574 amod reverse,primer
R2744 T4576 T4574 prep from,primer
R2745 T4577 T4578 amod known,sequence
R2746 T4578 T4576 pobj sequence,from
R2747 T4579 T4561 punct .,confirmed
R2748 T4581 T4582 mark Once,identified
R2749 T4582 T4589 advcl identified,obtained
R2750 T4583 T4582 nsubjpass most,identified
R2751 T4584 T4583 prep of,most
R2752 T4585 T4586 det the,sequences
R2753 T4586 T4584 pobj sequences,of
R2754 T4587 T4586 amod coding,sequences
R2755 T4588 T4582 auxpass were,identified
R2756 T4590 T4589 punct ", ",obtained
R2757 T4591 T4592 amod partial,sequence
R2758 T4592 T4589 nsubjpass sequence,obtained
R2759 T4593 T4592 prep of,sequence
R2760 T4594 T4595 nmod exon,sequences
R2761 T4595 T4593 pobj sequences,of
R2762 T4596 T4594 nummod 1,exon
R2763 T4597 T4594 cc and,exon
R2764 T4598 T4599 det the,UTR
R2765 T4599 T4594 conj UTR,exon
R2766 T4600 T4599 nummod 5,UTR
R2767 T4601 T4600 punct ',5
R2768 T4602 T4589 auxpass were,obtained
R2769 T4603 T4589 prep by,obtained
R2770 T4604 T4605 compound BAC,sequencing
R2771 T4605 T4603 pobj sequencing,by
R2772 T4606 T4605 compound DNA,sequencing
R2773 T4607 T4589 punct .,obtained
R2774 T4647 T4648 compound Northern,blot
R2775 T4648 T4649 compound blot,analyses
R2776 T4651 T4652 amod Multiple,filters
R2777 T4652 T4656 nsubjpass filters,purchased
R2778 T4653 T4652 compound Choice,filters
R2779 T4654 T4655 compound Northern,Blot
R2780 T4655 T4652 compound Blot,filters
R2781 T4657 T4652 acl containing,filters
R2782 T4658 T4659 nummod 12,tissues
R2783 T4659 T4657 dobj tissues,containing
R2784 T4660 T4659 amod different,tissues
R2785 T4661 T4659 compound mouse,tissues
R2786 T4662 T4656 auxpass were,purchased
R2787 T4663 T4656 prep from,purchased
R2788 T4664 T4663 pobj Origene,from
R2789 T4665 T4656 punct .,purchased
R2790 T4667 T4668 det The,filters
R2791 T4668 T4669 nsubjpass filters,probed
R2792 T4670 T4669 auxpass were,probed
R2793 T4671 T4669 prep for,probed
R2794 T4672 T4673 det each,gene
R2795 T4673 T4671 pobj gene,for
R2796 T4674 T4673 compound Acdp,gene
R2797 T4675 T4669 prep with,probed
R2798 T4676 T4677 det a,fragment
R2799 T4677 T4675 pobj fragment,with
R2800 T4678 T4677 compound PCR,fragment
R2801 T4679 T4680 punct (,bp
R2802 T4680 T4677 parataxis bp,fragment
R2803 T4681 T4682 quantmod around,350
R2804 T4682 T4680 nummod 350,bp
R2805 T4683 T4680 punct ),bp
R2806 T4684 T4677 prep from,fragment
R2807 T4685 T4686 amod last,exon
R2808 T4686 T4684 pobj exon,from
R2809 T4687 T4669 cc and,probed
R2810 T4688 T4689 det the,3
R2811 T4689 T4690 nummod 3,region
R2812 T4690 T4693 nsubj region,labeled
R2813 T4691 T4689 punct ',3
R2814 T4692 T4689 amod untranslated,3
R2815 T4693 T4669 conj labeled,probed
R2816 T4694 T4693 prep with,labeled
R2817 T4695 T4696 compound α,32P
R2818 T4696 T4698 compound 32P,dCTP
R2819 T4697 T4696 punct -,32P
R2820 T4698 T4694 pobj dCTP,with
R2821 T4699 T4693 advcl using,labeled
R2822 T4700 T4701 det the,system
R2823 T4701 T4699 dobj system,using
R2824 T4702 T4701 amod random,system
R2825 T4703 T4701 compound primer,system
R2826 T4704 T4701 compound extension,system
R2827 T4705 T4706 punct (,Technologies
R2828 T4706 T4701 parataxis Technologies,system
R2829 T4707 T4706 compound Life,Technologies
R2830 T4708 T4706 punct ),Technologies
R2831 T4709 T4693 punct .,labeled
R2832 T4711 T4712 nsubjpass Hybridizations,carried
R2833 T4713 T4712 auxpass were,carried
R2834 T4714 T4712 prt out,carried
R2835 T4715 T4712 advmod overnight,carried
R2836 T4716 T4712 punct .,carried
R2837 T4718 T4719 det The,filters
R2838 T4719 T4720 nsubjpass filters,washed
R2839 T4721 T4720 auxpass were,washed
R2840 T4722 T4720 advmod twice,washed
R2841 T4723 T4720 prep with,washed
R2842 T4724 T4725 amod washing,buffer
R2843 T4725 T4723 pobj buffer,with
R2844 T4726 T4725 nummod I,buffer
R2845 T4727 T4728 punct (,SDS
R2846 T4728 T4725 parataxis SDS,buffer
R2847 T4729 T4730 nummod 2,SSC
R2848 T4730 T4728 dep SSC,SDS
R2849 T4731 T4730 punct ×,SSC
R2850 T4732 T4728 punct ", ",SDS
R2851 T4733 T4734 nummod 0.1,%
R2852 T4734 T4728 compound %,SDS
R2853 T4735 T4728 punct ),SDS
R2854 T4736 T4720 prep at,washed
R2855 T4737 T4738 nummod 42,°C
R2856 T4738 T4736 pobj °C,at
R2857 T4739 T4720 prep for,washed
R2858 T4740 T4741 nummod 15,min
R2859 T4741 T4739 pobj min,for
R2860 T4742 T4741 punct -,min
R2861 T4743 T4720 punct ", ",washed
R2862 T4744 T4720 cc and,washed
R2863 T4745 T4746 advmod then,washed
R2864 T4746 T4720 conj washed,washed
R2865 T4747 T4746 advmod twice,washed
R2866 T4748 T4746 prep with,washed
R2867 T4749 T4750 compound washing,buffer
R2868 T4750 T4748 pobj buffer,with
R2869 T4751 T4750 nummod II,buffer
R2870 T4752 T4753 punct (,SDS
R2871 T4753 T4750 parataxis SDS,buffer
R2872 T4754 T4755 nummod 0.25,SSC
R2873 T4755 T4753 dep SSC,SDS
R2874 T4756 T4755 punct ×,SSC
R2875 T4757 T4753 punct ", ",SDS
R2876 T4758 T4759 nummod 0.1,%
R2877 T4759 T4753 compound %,SDS
R2878 T4760 T4753 punct ),SDS
R2879 T4761 T4746 prep at,washed
R2880 T4762 T4763 nummod 65,°C
R2881 T4763 T4761 pobj °C,at
R2882 T4764 T4746 prep for,washed
R2883 T4765 T4766 nummod 15,min
R2884 T4766 T4764 pobj min,for
R2885 T4767 T4766 punct -,min
R2886 T4768 T4720 punct .,washed
R2887 T4770 T4771 amod Washed,filters
R2888 T4771 T4772 nsubjpass filters,exposed
R2889 T4773 T4772 auxpass were,exposed
R2890 T4774 T4772 prep to,exposed
R2891 T4775 T4776 compound X-ray,films
R2892 T4776 T4774 pobj films,to
R2893 T4777 T4772 prep for,exposed
R2894 T4778 T4777 pcomp overnight,for
R2895 T4779 T4778 cc or,overnight
R2896 T4780 T4778 conj longer,overnight
R2897 T4781 T4782 punct [,4
R2898 T4782 T4772 parataxis 4,exposed
R2899 T4783 T4782 nummod 3,4
R2900 T4784 T4782 punct ",",4
R2901 T4785 T4782 punct ],4
R2902 T4786 T4772 punct .,exposed
R2904 T4823 T4824 compound Antibody,production
R2905 T4825 T4824 cc and,production
R2906 T4826 T4827 compound Western,blot
R2907 T4827 T4828 compound blot,analyses
R2908 T4828 T4824 conj analyses,production
R2909 T4830 T4831 nsubjpass Peptides,used
R2910 T4832 T4830 acl linked,Peptides
R2911 T4833 T4832 prep to,linked
R2912 T4834 T4833 pobj KLH,to
R2913 T4835 T4834 punct (,KLH
R2914 T4836 T4837 compound keyhole,hemacyanin
R2915 T4837 T4834 appos hemacyanin,KLH
R2916 T4838 T4837 compound limpet,hemacyanin
R2917 T4839 T4833 punct ),to
R2918 T4840 T4833 prep from,to
R2919 T4841 T4842 nmod Acdp1,terminals
R2920 T4842 T4840 pobj terminals,from
R2921 T4843 T4842 nmod N,terminals
R2922 T4844 T4843 punct -,N
R2923 T4845 T4843 cc and,N
R2924 T4846 T4843 conj C,N
R2925 T4847 T4842 punct -,terminals
R2926 T4848 T4842 cc and,terminals
R2927 T4849 T4850 det the,domain
R2928 T4850 T4842 conj domain,terminals
R2929 T4851 T4850 amod conserved,domain
R2930 T4852 T4850 punct (,domain
R2931 T4853 T4850 appos ACD,domain
R2932 T4854 T4831 punct ),used
R2933 T4855 T4831 auxpass were,used
R2934 T4856 T4831 prep for,used
R2935 T4857 T4856 pobj generation,for
R2936 T4858 T4857 prep of,generation
R2937 T4859 T4858 pobj antibodies,of
R2938 T4860 T4861 advmod specifically,for
R2939 T4861 T4856 prep for,for
R2940 T4862 T4861 pobj Acdp1,for
R2941 T4863 T4862 cc and,Acdp1
R2942 T4864 T4865 det all,members
R2943 T4865 T4862 conj members,Acdp1
R2944 T4866 T4865 compound Acdp,members
R2945 T4867 T4868 mark as,reported
R2946 T4868 T4831 advcl reported,used
R2947 T4869 T4831 punct ", ",used
R2948 T4870 T4831 advmod respectively,used
R2949 T4871 T4872 punct [,5
R2950 T4872 T4831 parataxis 5,used
R2951 T4873 T4872 punct ],5
R2952 T4874 T4831 punct .,used
R2953 T4876 T4877 compound Western,blots
R2954 T4877 T4878 nsubjpass blots,carried
R2955 T4879 T4878 auxpass were,carried
R2956 T4880 T4878 prt out,carried
R2957 T4881 T4878 advcl Using,carried
R2958 T4882 T4881 dobj ECL,Using
R2959 T4883 T4884 punct (,PIERCE
R2960 T4884 T4882 parataxis PIERCE,ECL
R2961 T4885 T4884 punct ),PIERCE
R2962 T4886 T4887 mark as,described
R2963 T4887 T4878 advcl described,carried
R2964 T4888 T4887 advmod previously,described
R2965 T4889 T4890 punct [,1
R2966 T4890 T4878 parataxis 1,carried
R2967 T4891 T4890 punct ],1
R2968 T4892 T4878 punct .,carried
R2969 T4894 T4895 det The,membranes
R2970 T4895 T4896 nsubjpass membranes,washed
R2971 T4897 T4896 auxpass were,washed
R2972 T4898 T4896 advmod extensively,washed
R2973 T4899 T4896 prep after,washed
R2974 T4900 T4899 pobj incubation,after
R2975 T4901 T4900 prep with,incubation
R2976 T4902 T4903 amod primary,antibodies
R2977 T4903 T4901 pobj antibodies,with
R2978 T4904 T4902 cc and,primary
R2979 T4905 T4902 conj secondary,primary
R2980 T4906 T4896 cc and,washed
R2981 T4907 T4908 auxpass were,developed
R2982 T4908 T4896 conj developed,washed
R2983 T4909 T4908 advmod then,developed
R2984 T4910 T4908 prep with,developed
R2985 T4911 T4912 compound X-ray,films
R2986 T4912 T4910 pobj films,with
R2987 T4913 T4912 prep with,films
R2988 T4914 T4915 amod optimal,time
R2989 T4915 T4913 pobj time,with
R2990 T4916 T4915 compound exposure,time
R2991 T4917 T4896 punct .,washed
R2992 T4941 T4942 compound Chromosome,localization
R2993 T4944 T4945 det The,panel
R2994 T4945 T4950 nsubjpass panel,used
R2995 T4946 T4945 compound T31,panel
R2996 T4947 T4945 compound mouse,panel
R2997 T4948 T4945 compound radiation,panel
R2998 T4949 T4945 compound hybrid,panel
R2999 T4951 T4945 prep from,panel
R3000 T4952 T4953 compound Research,Genetics
R3001 T4953 T4951 pobj Genetics,from
R3002 T4954 T4950 auxpass was,used
R3003 T4955 T4956 aux to,map
R3004 T4956 T4950 advcl map,used
R3005 T4957 T4958 det the,location
R3006 T4958 T4956 dobj location,map
R3007 T4959 T4958 compound chromosome,location
R3008 T4960 T4958 prep of,location
R3009 T4961 T4962 det each,member
R3010 T4962 T4960 pobj member,of
R3011 T4963 T4962 compound Acdp,member
R3012 T4964 T4950 punct .,used
R3013 T4966 T4967 nsubjpass Primers,used
R3014 T4968 T4966 prep from,Primers
R3015 T4969 T4970 nummod 3,UTR
R3016 T4970 T4968 pobj UTR,from
R3017 T4971 T4969 punct ',3
R3018 T4972 T4970 prep of,UTR
R3019 T4973 T4974 det each,member
R3020 T4974 T4972 pobj member,of
R3021 T4975 T4974 compound Acdp,member
R3022 T4976 T4967 auxpass were,used
R3023 T4977 T4978 aux to,amplify
R3024 T4978 T4967 advcl amplify,used
R3025 T4979 T4980 det the,clones
R3026 T4980 T4978 dobj clones,amplify
R3027 T4981 T4980 nummod 100,clones
R3028 T4982 T4980 nmod radiation,clones
R3029 T4983 T4980 amod hybrid,clones
R3030 T4984 T4980 acl representing,clones
R3031 T4985 T4986 det the,genome
R3032 T4986 T4984 dobj genome,representing
R3033 T4987 T4986 compound mouse,genome
R3034 T4988 T4967 punct .,used
R3035 T4990 T4991 det The,data
R3036 T4991 T4992 nsubjpass data,submitted
R3037 T4993 T4992 auxpass were,submitted
R3038 T4994 T4992 prep to,submitted
R3039 T4995 T4996 det the,Database
R3040 T4996 T4994 pobj Database,to
R3041 T4997 T4998 compound Jackson,Laboratory
R3042 T4998 T4996 compound Laboratory,Database
R3043 T4999 T5000 compound Mouse,Hybrid
R3044 T5000 T4996 compound Hybrid,Database
R3045 T5001 T5000 compound Radiation,Hybrid
R3046 T5002 T4992 prep for,submitted
R3047 T5003 T5002 pobj analysis,for
R3048 T5004 T4992 punct .,submitted
R3049 T5023 T5024 compound Sequence,analyses
R3050 T5026 T5027 compound Sequence,assembly
R3051 T5027 T5028 nsubjpass assembly,performed
R3052 T5029 T5028 auxpass was,performed
R3053 T5030 T5028 prep with,performed
R3054 T5031 T5032 compound program,Sequencher
R3055 T5032 T5030 pobj Sequencher,with
R3056 T5033 T5034 punct (,Corp
R3057 T5034 T5032 parataxis Corp,Sequencher
R3058 T5035 T5034 compound Gene,Corp
R3059 T5036 T5034 compound Codes,Corp
R3060 T5037 T5034 punct ),Corp
R3061 T5038 T5028 punct .,performed
R3062 T5040 T5041 nmod Protein,homology
R3063 T5041 T5044 compound homology,searches
R3064 T5042 T5040 cc and,Protein
R3065 T5043 T5040 conj DNA,Protein
R3066 T5044 T5045 nsubjpass searches,carried
R3067 T5046 T5045 auxpass were,carried
R3068 T5047 T5045 prt out,carried
R3069 T5048 T5045 prep with,carried
R3070 T5049 T5050 nmod tblastn,programs
R3071 T5050 T5048 pobj programs,with
R3072 T5051 T5049 punct ", ",tblastn
R3073 T5052 T5049 conj tblastx,tblastn
R3074 T5053 T5052 punct ", ",tblastx
R3075 T5054 T5052 conj blastp,tblastx
R3076 T5055 T5054 cc and,blastp
R3077 T5056 T5054 conj blastn,blastp
R3078 T5057 T5045 punct .,carried
R3079 T5059 T5060 amod Multiple,alignments
R3080 T5060 T5062 nsubjpass alignments,performed
R3081 T5061 T5060 compound sequence,alignments
R3082 T5063 T5062 auxpass were,performed
R3083 T5064 T5062 prep with,performed
R3084 T5065 T5066 nmod GeneDoc,alignment
R3085 T5066 T5064 pobj alignment,with
R3086 T5067 T5065 cc and,GeneDoc
R3087 T5068 T5065 conj pairwise,GeneDoc
R3088 T5069 T5066 compound sequence,alignment
R3089 T5070 T5062 punct .,performed
R3090 T5072 T5073 amod Multiple,programs
R3091 T5073 T5074 nsubjpass programs,used
R3092 T5075 T5073 prep including,programs
R3093 T5076 T5077 compound BCM,Launcher
R3094 T5077 T5075 pobj Launcher,including
R3095 T5078 T5077 compound Search,Launcher
R3096 T5079 T5077 punct ", ",Launcher
R3097 T5080 T5077 conj ProfileScan,Launcher
R3098 T5081 T5080 punct ", ",ProfileScan
R3099 T5082 T5083 compound sequence,search
R3100 T5083 T5080 conj search,ProfileScan
R3101 T5084 T5083 compound motif,search
R3102 T5085 T5083 punct ", ",search
R3103 T5086 T5083 conj ExPASy,search
R3104 T5087 T5086 cc and,ExPASy
R3105 T5088 T5086 conj 3Dpssm,ExPASy
R3106 T5089 T5074 auxpass were,used
R3107 T5090 T5074 prep for,used
R3108 T5091 T5090 pcomp searching,for
R3109 T5092 T5093 compound sequence,features
R3110 T5093 T5091 dobj features,searching
R3111 T5094 T5093 prep of,features
R3112 T5095 T5096 amod known,protein
R3113 T5096 T5094 pobj protein,of
R3114 T5097 T5074 punct .,used
R3115 T5099 T5100 amod Phylogenetic,tree
R3116 T5100 T5101 nsubjpass tree,constructed
R3117 T5102 T5101 auxpass was,constructed
R3118 T5103 T5101 agent by,constructed
R3119 T5104 T5105 compound Clustalw,program
R3120 T5105 T5103 pobj program,by
R3121 T5106 T5107 punct (,version
R3122 T5107 T5105 parataxis version,program
R3123 T5108 T5107 nummod 1.81,version
R3124 T5109 T5107 punct ),version
R3125 T5110 T5101 advcl using,constructed
R3126 T5111 T5112 nmod UPGMA,algorithm
R3127 T5112 T5110 dobj algorithm,using
R3128 T5113 T5111 punct (,UPGMA
R3129 T5114 T5115 amod Unweighted,Method
R3130 T5115 T5111 appos Method,UPGMA
R3131 T5116 T5115 compound Pair,Method
R3132 T5117 T5115 compound Group,Method
R3133 T5118 T5115 acl using,Method
R3134 T5119 T5120 compound Arithmetic,averages
R3135 T5120 T5118 dobj averages,using
R3136 T5121 T5112 punct ),algorithm
R3137 T5122 T5123 punct [,14
R3138 T5123 T5101 parataxis 14,constructed
R3139 T5124 T5123 punct ],14
R3140 T5125 T5101 punct .,constructed
R3141 T5264 T5265 amod Neuronal,preparation
R3142 T5266 T5265 compound cell,preparation
R3143 T5267 T5265 cc and,preparation
R3144 T5268 T5265 conj immunostanining,preparation
R3145 T5270 T5271 amod Hippocampal,cultures
R3146 T5271 T5273 nsubjpass cultures,prepared
R3147 T5272 T5271 compound neuron,cultures
R3148 T5274 T5273 auxpass were,prepared
R3149 T5275 T5276 mark as,reported
R3150 T5276 T5273 advcl reported,prepared
R3151 T5277 T5276 advmod previously,reported
R3152 T5278 T5279 punct [,6
R3153 T5279 T5273 parataxis 6,prepared
R3154 T5280 T5279 punct ],6
R3155 T5281 T5273 punct .,prepared
R3156 T5283 T5284 prep In,dissected
R3157 T5285 T5283 pobj brief,In
R3158 T5286 T5284 punct ", ",dissected
R3159 T5287 T5288 det the,hippocampuses
R3160 T5288 T5284 nsubjpass hippocampuses,dissected
R3161 T5289 T5284 auxpass were,dissected
R3162 T5290 T5284 prt out,dissected
R3163 T5291 T5284 prep from,dissected
R3164 T5292 T5293 compound mouse,embryos
R3165 T5293 T5291 pobj embryos,from
R3166 T5294 T5284 prep at,dissected
R3167 T5295 T5296 nummod 16,days
R3168 T5296 T5294 pobj days,at
R3169 T5297 T5298 advmod in,utero
R3170 T5298 T5296 advmod utero,days
R3171 T5299 T5284 punct .,dissected
R3172 T5301 T5302 det The,tissues
R3173 T5302 T5303 nsubjpass tissues,incubated
R3174 T5304 T5303 auxpass were,incubated
R3175 T5305 T5303 advmod then,incubated
R3176 T5306 T5303 prep for,incubated
R3177 T5307 T5308 nummod 20,min
R3178 T5308 T5306 pobj min,for
R3179 T5309 T5303 prep at,incubated
R3180 T5310 T5311 nummod 37,°C
R3181 T5311 T5309 pobj °C,at
R3182 T5312 T5303 prep in,incubated
R3183 T5313 T5312 pobj MEM,in
R3184 T5314 T5313 punct (,MEM
R3185 T5315 T5316 amod minimum,medium
R3186 T5316 T5313 appos medium,MEM
R3187 T5317 T5316 amod essential,medium
R3188 T5318 T5313 punct ),MEM
R3189 T5319 T5313 acl modified,MEM
R3190 T5320 T5319 prep for,modified
R3191 T5321 T5322 compound suspension,culture
R3192 T5322 T5320 pobj culture,for
R3193 T5323 T5324 punct (,Technologies
R3194 T5324 T5313 parataxis Technologies,MEM
R3195 T5325 T5324 compound Life,Technologies
R3196 T5326 T5324 punct ),Technologies
R3197 T5327 T5313 cc plus,MEM
R3198 T5328 T5329 nummod 0.25,%
R3199 T5329 T5330 compound %,trypsin
R3200 T5330 T5313 conj trypsin,MEM
R3201 T5331 T5332 punct (,Technologies
R3202 T5332 T5330 parataxis Technologies,trypsin
R3203 T5333 T5332 compound Life,Technologies
R3204 T5334 T5332 punct ),Technologies
R3205 T5335 T5303 punct .,incubated
R3206 T5337 T5338 det The,neurons
R3207 T5338 T5341 nsubjpass neurons,plated
R3208 T5339 T5338 amod dissociated,neurons
R3209 T5340 T5338 amod hippocampal,neurons
R3210 T5342 T5341 auxpass were,plated
R3211 T5343 T5341 prep on,plated
R3212 T5344 T5345 compound glass,coverslips
R3213 T5345 T5343 pobj coverslips,on
R3214 T5346 T5345 acl coated,coverslips
R3215 T5347 T5346 prep with,coated
R3216 T5348 T5349 det a,monolayer
R3217 T5349 T5347 pobj monolayer,with
R3218 T5350 T5349 amod confluent,monolayer
R3219 T5351 T5349 prep of,monolayer
R3220 T5352 T5353 nmod mouse,astrocytes
R3221 T5353 T5351 pobj astrocytes,of
R3222 T5354 T5353 amod cortical,astrocytes
R3223 T5355 T5353 acl obtained,astrocytes
R3224 T5356 T5357 mark as,described
R3225 T5357 T5355 advcl described,obtained
R3226 T5358 T5357 advmod below,described
R3227 T5359 T5341 punct .,plated
R3228 T5361 T5362 det The,neurons
R3229 T5362 T5363 nsubjpass neurons,maintained
R3230 T5364 T5363 auxpass were,maintained
R3231 T5365 T5363 prep at,maintained
R3232 T5366 T5367 nummod 37,°C
R3233 T5367 T5365 pobj °C,at
R3234 T5368 T5363 prep in,maintained
R3235 T5369 T5370 det a,atmosphere
R3236 T5370 T5368 pobj atmosphere,in
R3237 T5371 T5370 amod humidified,atmosphere
R3238 T5372 T5370 prep with,atmosphere
R3239 T5373 T5374 nummod 5,%
R3240 T5374 T5375 compound %,CO2
R3241 T5375 T5372 pobj CO2,with
R3242 T5376 T5363 punct .,maintained
R3243 T5378 T5379 amod Cortical,astrocytes
R3244 T5379 T5380 nsubjpass astrocytes,grown
R3245 T5381 T5379 acl dissociated,astrocytes
R3246 T5382 T5381 prep from,dissociated
R3247 T5383 T5384 amod newborn,mouse
R3248 T5384 T5385 compound mouse,cortices
R3249 T5385 T5382 pobj cortices,from
R3250 T5386 T5380 auxpass were,grown
R3251 T5387 T5380 prep in,grown
R3252 T5388 T5389 compound culture,flasks
R3253 T5389 T5387 pobj flasks,in
R3254 T5390 T5380 prep at,grown
R3255 T5391 T5392 nummod 37,°C
R3256 T5392 T5390 pobj °C,at
R3257 T5393 T5380 prep in,grown
R3258 T5394 T5395 det a,atmosphere
R3259 T5395 T5393 pobj atmosphere,in
R3260 T5396 T5395 amod humidified,atmosphere
R3261 T5397 T5395 prep with,atmosphere
R3262 T5398 T5399 nummod 5,%
R3263 T5399 T5400 compound %,CO2
R3264 T5400 T5397 pobj CO2,with
R3265 T5401 T5380 prep until,grown
R3266 T5402 T5401 pcomp confluent,until
R3267 T5403 T5380 punct .,grown
R3268 T5405 T5406 det The,cells
R3269 T5406 T5407 nsubjpass cells,exposed
R3270 T5408 T5407 auxpass were,exposed
R3271 T5409 T5407 prep to,exposed
R3272 T5410 T5411 quantmod 10,5
R3273 T5411 T5413 nummod 5,M
R3274 T5412 T5411 punct -,5
R3275 T5413 T5414 compound M,arabinoside
R3276 T5414 T5409 pobj arabinoside,to
R3277 T5415 T5414 compound cytosine,arabinoside
R3278 T5416 T5417 punct (,Sigma
R3279 T5417 T5414 parataxis Sigma,arabinoside
R3280 T5418 T5417 punct ),Sigma
R3281 T5419 T5407 cc and,exposed
R3282 T5420 T5407 conj cultured,exposed
R3283 T5421 T5420 prep for,cultured
R3284 T5422 T5423 amod additional,hrs
R3285 T5423 T5421 pobj hrs,for
R3286 T5424 T5425 quantmod 12,24
R3287 T5425 T5423 nummod 24,hrs
R3288 T5426 T5425 punct –,24
R3289 T5427 T5420 prep at,cultured
R3290 T5428 T5429 nummod 37,°C
R3291 T5429 T5427 pobj °C,at
R3292 T5430 T5407 punct .,exposed
R3293 T5432 T5433 prep After,used
R3294 T5434 T5432 pobj remove,After
R3295 T5435 T5434 prep of,remove
R3296 T5436 T5437 det the,media
R3297 T5437 T5435 pobj media,of
R3298 T5438 T5434 prep with,remove
R3299 T5439 T5440 amod cellular,debris
R3300 T5440 T5438 pobj debris,with
R3301 T5441 T5433 punct ", ",used
R3302 T5442 T5443 det the,cells
R3303 T5443 T5433 nsubjpass cells,used
R3304 T5444 T5433 auxpass were,used
R3305 T5445 T5433 prep for,used
R3306 T5446 T5445 pcomp coating,for
R3307 T5447 T5446 dobj coverslips,coating
R3308 T5448 T5433 punct .,used
R3309 T5450 T5451 prep For,fixed
R3310 T5452 T5450 pobj immunostaining,For
R3311 T5453 T5451 punct ", ",fixed
R3312 T5454 T5455 amod neuronal,cells
R3313 T5455 T5451 nsubjpass cells,fixed
R3314 T5456 T5455 prep on,cells
R3315 T5457 T5458 det the,coverslips
R3316 T5458 T5456 pobj coverslips,on
R3317 T5459 T5451 auxpass were,fixed
R3318 T5460 T5451 advmod first,fixed
R3319 T5461 T5451 prep in,fixed
R3320 T5462 T5461 pobj PBS,in
R3321 T5463 T5462 acl containing,PBS
R3322 T5464 T5465 nummod 4,%
R3323 T5465 T5466 compound %,paraformaldehyde
R3324 T5466 T5463 dobj paraformaldehyde,containing
R3325 T5467 T5466 punct (,paraformaldehyde
R3326 T5468 T5466 appos PFA,paraformaldehyde
R3327 T5469 T5463 punct ),containing
R3328 T5470 T5463 prep for,containing
R3329 T5471 T5472 nummod 12,hrs
R3330 T5472 T5470 pobj hrs,for
R3331 T5473 T5463 prep at,containing
R3332 T5474 T5475 nummod 4,°C
R3333 T5475 T5473 pobj °C,at
R3334 T5476 T5451 cc and,fixed
R3335 T5477 T5478 advmod then,incubated
R3336 T5478 T5451 conj incubated,fixed
R3337 T5479 T5478 prep in,incubated
R3338 T5480 T5481 det a,solution
R3339 T5481 T5479 pobj solution,in
R3340 T5482 T5481 acl containing,solution
R3341 T5483 T5484 nummod 4,%
R3342 T5484 T5485 compound %,PFA
R3343 T5485 T5482 dobj PFA,containing
R3344 T5486 T5485 cc and,PFA
R3345 T5487 T5488 nummod 0.4,%
R3346 T5488 T5489 compound %,100
R3347 T5489 T5485 conj 100,PFA
R3348 T5490 T5489 compound Triton,100
R3349 T5491 T5489 compound X,100
R3350 T5492 T5489 punct -,100
R3351 T5493 T5478 prep at,incubated
R3352 T5494 T5495 nummod 4,°C
R3353 T5495 T5493 pobj °C,at
R3354 T5496 T5478 prep for,incubated
R3355 T5497 T5498 nummod 1,hr
R3356 T5498 T5496 pobj hr,for
R3357 T5499 T5451 punct .,fixed
R3358 T5501 T5502 prep After,incubated
R3359 T5503 T5501 pcomp washing,After
R3360 T5504 T5503 prep with,washing
R3361 T5505 T5504 pobj PBS,with
R3362 T5506 T5507 nummod three,times
R3363 T5507 T5503 npadvmod times,washing
R3364 T5508 T5502 punct ", ",incubated
R3365 T5509 T5510 det the,cells
R3366 T5510 T5502 nsubjpass cells,incubated
R3367 T5511 T5502 auxpass were,incubated
R3368 T5512 T5502 prep with,incubated
R3369 T5513 T5514 det a,solution
R3370 T5514 T5512 pobj solution,with
R3371 T5515 T5514 amod blocking,solution
R3372 T5516 T5514 acl containing,solution
R3373 T5517 T5518 nummod 1,serum
R3374 T5518 T5516 dobj serum,containing
R3375 T5519 T5520 punct :,30
R3376 T5520 T5517 prep 30,1
R3377 T5521 T5518 amod normal,serum
R3378 T5522 T5518 compound goat,serum
R3379 T5523 T5502 punct ", ",incubated
R3380 T5524 T5502 cc and,incubated
R3381 T5525 T5526 advmod subsequently,incubated
R3382 T5526 T5502 conj incubated,incubated
R3383 T5527 T5526 prep with,incubated
R3384 T5528 T5529 det a,antibody
R3385 T5529 T5527 pobj antibody,with
R3386 T5530 T5529 nmod rabbit,antibody
R3387 T5531 T5529 amod polyclonal,antibody
R3388 T5532 T5529 amod anti-ACDP,antibody
R3389 T5533 T5534 punct (,1
R3390 T5534 T5529 parataxis 1,antibody
R3391 T5535 T5536 punct :,3000
R3392 T5536 T5534 prep 3000,1
R3393 T5537 T5534 punct ),1
R3394 T5538 T5526 advmod overnight,incubated
R3395 T5539 T5526 prep at,incubated
R3396 T5540 T5541 nummod 4,°C
R3397 T5541 T5539 pobj °C,at
R3398 T5542 T5502 punct .,incubated
R3399 T5544 T5545 prep After,incubated
R3400 T5546 T5547 amod extensive,washing
R3401 T5547 T5544 pobj washing,After
R3402 T5548 T5547 prep with,washing
R3403 T5549 T5550 nummod 1,%
R3404 T5550 T5551 compound %,serum
R3405 T5551 T5553 compound serum,solution
R3406 T5552 T5551 compound goat,serum
R3407 T5553 T5548 pobj solution,with
R3408 T5554 T5553 compound PBS,solution
R3409 T5555 T5545 punct ", ",incubated
R3410 T5556 T5557 det the,cells
R3411 T5557 T5545 nsubjpass cells,incubated
R3412 T5558 T5545 auxpass were,incubated
R3413 T5559 T5545 prep with,incubated
R3414 T5560 T5561 det an,antibody
R3415 T5561 T5559 pobj antibody,with
R3416 T5562 T5561 nmod Alex,antibody
R3417 T5563 T5562 nummod 488,Alex
R3418 T5564 T5561 amod conjugated,antibody
R3419 T5565 T5561 amod secondary,antibody
R3420 T5566 T5567 punct (,Probes
R3421 T5567 T5561 parataxis Probes,antibody
R3422 T5568 T5567 dep 1,Probes
R3423 T5569 T5570 punct :,100
R3424 T5570 T5568 prep 100,1
R3425 T5571 T5568 prep in,1
R3426 T5572 T5573 nummod 1,%
R3427 T5573 T5574 compound %,serum
R3428 T5574 T5576 compound serum,solution
R3429 T5575 T5574 compound goat,serum
R3430 T5576 T5571 pobj solution,in
R3431 T5577 T5576 compound PBS,solution
R3432 T5578 T5567 punct ", ",Probes
R3433 T5579 T5567 compound Molecular,Probes
R3434 T5580 T5567 punct ),Probes
R3435 T5581 T5545 prep for,incubated
R3436 T5582 T5583 nummod 3,hrs
R3437 T5583 T5581 pobj hrs,for
R3438 T5584 T5545 prep at,incubated
R3439 T5585 T5586 compound room,temperature
R3440 T5586 T5584 pobj temperature,at
R3441 T5587 T5545 punct .,incubated
R3442 T5589 T5590 prep Following,slipped
R3443 T5591 T5592 amod final,washes
R3444 T5592 T5589 pobj washes,Following
R3445 T5593 T5592 prep with,washes
R3446 T5594 T5595 nummod 1,%
R3447 T5595 T5596 compound %,serum
R3448 T5596 T5598 compound serum,solution
R3449 T5597 T5596 compound goat,serum
R3450 T5598 T5593 pobj solution,with
R3451 T5599 T5598 compound PBS,solution
R3452 T5600 T5590 punct ", ",slipped
R3453 T5601 T5602 det the,cells
R3454 T5602 T5590 nsubjpass cells,slipped
R3455 T5603 T5602 amod neuronal,cells
R3456 T5604 T5602 prep on,cells
R3457 T5605 T5606 det the,coverslips
R3458 T5606 T5604 pobj coverslips,on
R3459 T5607 T5590 auxpass were,slipped
R3460 T5608 T5590 dep cover,slipped
R3461 T5609 T5590 punct -,slipped
R3462 T5610 T5590 prep with,slipped
R3463 T5611 T5612 det a,medium
R3464 T5612 T5610 pobj medium,with
R3465 T5613 T5614 npadvmod glycerol,based
R3466 T5614 T5612 amod based,medium
R3467 T5615 T5614 punct -,based
R3468 T5616 T5612 amod anti-photobleach,medium
R3469 T5617 T5590 punct .,slipped
R3470 T5619 T5620 det The,cells
R3471 T5620 T5621 nsubjpass cells,viewed
R3472 T5622 T5621 auxpass were,viewed
R3473 T5623 T5621 prep under,viewed
R3474 T5624 T5625 det a,microscope
R3475 T5625 T5623 pobj microscope,under
R3476 T5626 T5625 amod confocal,microscope
R3477 T5627 T5628 punct (,Zeiss
R3478 T5628 T5625 parataxis Zeiss,microscope
R3479 T5629 T5628 compound Carl,Zeiss
R3480 T5630 T5628 punct ),Zeiss
R3481 T5631 T5621 punct .,viewed
R3482 T5633 T5634 nsubjpass Images,captured
R3483 T5635 T5634 auxpass were,captured
R3484 T5636 T5634 prep with,captured
R3485 T5637 T5638 det a,camera
R3486 T5638 T5636 pobj camera,with
R3487 T5639 T5638 compound CCD,camera
R3488 T5640 T5634 cc and,captured
R3489 T5641 T5634 conj acquired,captured
R3490 T5642 T5641 prep by,acquired
R3491 T5643 T5644 det the,software
R3492 T5644 T5642 pobj software,by
R3493 T5645 T5644 compound Scion,software
R3494 T5646 T5644 compound Image,software
R3495 T5647 T5648 punct (,Corporation
R3496 T5648 T5644 parataxis Corporation,software
R3497 T5649 T5648 compound Scion,Corporation
R3498 T5650 T5648 punct ", ",Corporation
R3499 T5651 T5648 npadvmod Frederick,Corporation
R3500 T5652 T5648 punct ", ",Corporation
R3501 T5653 T5648 npadvmod MD,Corporation
R3502 T5654 T5648 punct ),Corporation
R3503 T5655 T5634 punct .,captured
R3504 T5677 T5678 compound Gene,bank
R3505 T5678 T5679 compound bank,number
R3506 T5680 T5679 compound accession,number
R3507 T5682 T5683 det The,sequences
R3508 T5683 T5685 nsubjpass sequences,deposited
R3509 T5684 T5683 compound cDNA,sequences
R3510 T5686 T5683 prep for,sequences
R3511 T5687 T5688 det the,family
R3512 T5688 T5686 pobj family,for
R3513 T5689 T5688 compound Acdp,family
R3514 T5690 T5688 compound gene,family
R3515 T5691 T5685 aux have,deposited
R3516 T5692 T5685 advmod already,deposited
R3517 T5693 T5685 auxpass been,deposited
R3518 T5694 T5685 prep in,deposited
R3519 T5695 T5696 compound gene,bank
R3520 T5696 T5694 pobj bank,in
R3521 T5697 T5685 prep with,deposited
R3522 T5698 T5699 compound accession,AF202994
R3523 T5699 T5697 pobj AF202994,with
R3524 T5700 T5699 compound numbers,AF202994
R3525 T5701 T5699 punct (,AF202994
R3526 T5702 T5699 appos Acdp1,AF202994
R3527 T5703 T5699 punct ),AF202994
R3528 T5704 T5699 punct ", ",AF202994
R3529 T5705 T5699 conj AF216961,AF202994
R3530 T5706 T5705 punct (,AF216961
R3531 T5707 T5705 appos Acdp2,AF216961
R3532 T5708 T5705 punct ),AF216961
R3533 T5709 T5705 punct ", ",AF216961
R3534 T5710 T5705 conj AF216964,AF216961
R3535 T5711 T5710 punct (,AF216964
R3536 T5712 T5710 appos Acdp3,AF216964
R3537 T5713 T5710 punct ),AF216964
R3538 T5714 T5710 cc and,AF216964
R3539 T5715 T5710 conj AF216963,AF216964
R3540 T5716 T5715 punct (,AF216963
R3541 T5717 T5715 appos Acdp4,AF216963
R3542 T5718 T5699 punct ),AF202994
R3543 T5719 T5685 punct .,deposited

craft-ca-core-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5666 5437-5442 PR:000005646 denotes Acdp4
T5665 5416-5421 PR:000005645 denotes Acdp3
T5664 5398-5403 PR:000005644 denotes Acdp2
T5663 5380-5385 PR:000005643 denotes Acdp1
T5662 5337-5341 SO:0000704 denotes gene
T5661 5294-5298 SO:0000704 denotes gene
T5660 5235-5239 SO:0000704 denotes Gene
T5207 5014-5022 CHEBI:17754 denotes glycerol
T5206 4955-4969 CL:0000540 denotes neuronal cells
T5205 4941-4949 CHEBI:75958 denotes solution
T5204 4926-4930 NCBITaxon:9925 denotes goat
T5203 4836-4844 CHEBI:75958 denotes solution
T5202 4821-4825 NCBITaxon:9925 denotes goat
T5201 4799-4807 GO:0042571 denotes antibody
T5200 4778-4788 MOP:0000779 denotes conjugated
T5199 4769-4777 CHEBI:52661 denotes Alex 488
T5198 4726-4734 CHEBI:75958 denotes solution
T5197 4711-4715 NCBITaxon:9925 denotes goat
T5196 4643-4651 GO:0042571 denotes antibody
T5195 4615-4621 NCBITaxon:9986 denotes rabbit
T5194 4569-4573 NCBITaxon:9925 denotes goat
T5193 4537-4545 CHEBI:75958 denotes solution
T5192 4430-4442 CHEBI:9750 denotes Triton X-100
T5191 4394-4402 CHEBI:75958 denotes solution
T5190 4258-4272 CL:0000540 denotes neuronal cells
T5189 4070-4090 CHEBI:28680 denotes cytosine arabinoside
T5188 4016-4019 CHEBI:16526 denotes CO2
T5187 3949-3954 GO:0040007 denotes grown
T5186 3935-3943 UBERON:0001851 denotes cortices
T5185 3929-3934 NCBITaxon:10088 denotes mouse
T5184 3884-3903 CL:0002605 denotes Cortical astrocytes
T5183 3884-3892 UBERON:0001851 denotes Cortical
T5182 3879-3882 CHEBI:16526 denotes CO2
T5181 3812-3819 CL:0000540 denotes neurons
T5180 3759-3778 CL:0002605 denotes cortical astrocytes
T5179 3759-3767 UBERON:0001851 denotes cortical
T5178 3753-3758 NCBITaxon:10088 denotes mouse
T5177 3664-3683 CL:0002608 denotes hippocampal neurons
T5176 3619-3626 PR:000027795 denotes trypsin
T5175 3475-3482 UBERON:0000479 denotes tissues
T5174 3442-3449 UBERON:0000922 denotes embryos
T5173 3436-3441 NCBITaxon:10088 denotes mouse
T5172 3314-3332 CL:0002608 denotes Hippocampal neuron
T5171 3268-3281 CL:0000540 denotes Neuronal cell
T5009 3077-3094 SO:0000110 denotes sequence features
T5008 2767-2775 SO:0000857 denotes homology
T5007 2733-2737 SO:0000704 denotes Gene
T4928 2611-2616 NCBITaxon:10088 denotes Mouse
T4927 2553-2559 SO:0001026 denotes genome
T4926 2547-2552 NCBITaxon:10088 denotes mouse
T4925 2450-2456 SO:0000205 denotes 3' UTR
T4924 2437-2444 SO:0000112 denotes Primers
T4923 2324-2329 NCBITaxon:10088 denotes mouse
T4805 2211-2221 GO:0042571 denotes antibodies
T4804 1992-1997 PR:000005643 denotes Acdp1
T4803 1964-1974 GO:0042571 denotes antibodies
T4802 1923-1929 SO:0000417 denotes domain
T4801 1880-1885 PR:000005643 denotes Acdp1
T4800 1856-1862 NCBITaxon:6462 denotes limpet
T4799 1833-1839 MOP:0000779 denotes linked
T4798 1778-1786 GO:0042571 denotes Antibody
T4627 1678-1681 CHEBI:8984 denotes SDS
T4626 1592-1595 CHEBI:8984 denotes SDS
T4625 1484-1498 GO:0097617 denotes Hybridizations
T4624 1439-1445 SO:0000112 denotes primer
T4623 1413-1416 CHEBI:37972 denotes 32P
T4622 1380-1390 GO:0006412 denotes translated
T4621 1375-1397 SO:0000205 denotes 3' untranslated region
T4620 1362-1366 SO:0000147 denotes exon
T4619 1348-1350 SO:0000028 denotes bp
T4618 1311-1315 SO:0000704 denotes gene
T4617 1236-1243 UBERON:0000479 denotes tissues
T4616 1230-1235 NCBITaxon:10088 denotes mouse
T4332 1124-1127 SO:0000153 denotes BAC
T4331 1090-1096 SO:0000204 denotes 5' UTR
T4330 1075-1079 SO:0000147 denotes exon
T4329 968-982 SO:0000132 denotes reverse primer
T4328 916-930 SO:0000121 denotes forward primer
T4327 813-820 CHEBI:2511 denotes agarose
T4326 773-785 SO:0000006 denotes PCR products
T4325 730-739 GO:0097617 denotes annealing
T4324 709-721 SO:0000006 denotes PCR products
T4323 683-687 SO:0000704 denotes gene
T4322 677-682 NCBITaxon:10088 denotes mouse
T4321 617-631 SO:0000121 denotes forward primer
T4320 611-616 NCBITaxon:9606 denotes human
T4319 585-588 SO:0000345 denotes EST
T4318 579-584 NCBITaxon:10088 denotes mouse
T4317 555-569 SO:0000132 denotes reverse primer
T4316 493-502 GO:0097617 denotes annealing
T4315 470-475 NCBITaxon:10088 denotes mouse
T4314 446-455 SO:0000853 denotes homologue
T4313 410-413 SO:0000345 denotes EST
T4312 404-409 NCBITaxon:10088 denotes mouse
T4311 380-394 SO:0000132 denotes reverse primer
T4310 374-379 NCBITaxon:10088 denotes mouse
T4309 355-366 SO:0000318 denotes start codon
T4308 315-320 NCBITaxon:9606 denotes human
T4307 289-303 SO:0000121 denotes forward primer
T4306 240-243 SO:0000345 denotes EST
T4305 223-226 SO:0000345 denotes EST
T4304 217-222 NCBITaxon:10088 denotes mouse
T4303 149-154 NCBITaxon:9606 denotes human
T4302 88-97 SO:0000853 denotes homologue
T4301 82-87 NCBITaxon:9606 denotes human
T4300 47-51 SO:0000704 denotes gene

2_test

Id Subject Object Predicate Lexical cue
14723793-10049587-10642194 132-133 10049587 denotes 2
14723793-11595174-10642195 1771-1772 11595174 denotes 3
14723793-10550218-10642196 1773-1774 10550218 denotes 4
14723793-11533054-10642197 2046-2047 11533054 denotes 5
14723793-12657465-10642198 2125-2126 12657465 denotes 1
14723793-7984417-10642199 3262-3264 7984417 denotes 14
14723793-8657283-10642200 3380-3381 8657283 denotes 6

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the chain model. You can change it to the bag model.

Id Subject Object Predicate Lexical cue
T5676 5437-5442 PR_EXT:000005646 denotes Acdp4
T5675 5416-5421 PR_EXT:000005645 denotes Acdp3
T5674 5398-5403 PR_EXT:000005644 denotes Acdp2
T5673 5380-5385 PR_EXT:000005643 denotes Acdp1
T5672 5337-5341 SO_EXT:0000704 denotes gene
T5671 5294-5298 SO_EXT:0000704 denotes gene
T5670 5289-5293 PR_EXT:ACDP_or_CNNM denotes Acdp
T5669 5271-5280 SO_EXT:biological_sequence denotes sequences
T5668 5266-5270 SO_EXT:cDNA denotes cDNA
T5667 5235-5239 SO_EXT:0000704 denotes Gene
T5263 5058-5063 CL_GO_EXT:cell denotes cells
T5262 5014-5022 CHEBI:17754 denotes glycerol
T5261 4964-4969 CL_GO_EXT:cell denotes cells
T5260 4955-4969 CL:0000540 denotes neuronal cells
T5259 4941-4949 CHEBI:75958 denotes solution
T5258 4926-4930 NCBITaxon:9925 denotes goat
T5257 4856-4862 CHEBI_SO_EXT:molecular_probe denotes Probes
T5256 4846-4855 CHEBI_EXT:polyatomic_entity_or_group denotes Molecular
T5255 4836-4844 CHEBI:75958 denotes solution
T5254 4821-4825 NCBITaxon:9925 denotes goat
T5253 4799-4807 GO:0042571 denotes antibody
T5252 4778-4788 MOP:0000779 denotes conjugated
T5251 4769-4777 CHEBI:52661 denotes Alex 488
T5250 4740-4745 CL_GO_EXT:cell denotes cells
T5249 4726-4734 CHEBI:75958 denotes solution
T5248 4711-4715 NCBITaxon:9925 denotes goat
T5247 4643-4651 GO:0042571 denotes antibody
T5246 4638-4642 PR_EXT:ACDP_or_CNNM denotes ACDP
T5245 4615-4621 NCBITaxon:9986 denotes rabbit
T5244 4569-4573 NCBITaxon:9925 denotes goat
T5243 4537-4545 CHEBI:75958 denotes solution
T5242 4500-4505 CL_GO_EXT:cell denotes cells
T5241 4430-4442 CHEBI:9750 denotes Triton X-100
T5240 4417-4420 CHEBI_EXT:paraformaldehye denotes PFA
T5239 4394-4402 CHEBI:75958 denotes solution
T5238 4347-4350 CHEBI_EXT:paraformaldehye denotes PFA
T5237 4329-4345 CHEBI_EXT:paraformaldehye denotes paraformaldehyde
T5236 4267-4272 CL_GO_EXT:cell denotes cells
T5235 4258-4272 CL:0000540 denotes neuronal cells
T5234 4198-4203 CL_GO_EXT:cell denotes cells
T5233 4177-4185 CL_GO_EXT:cell denotes cellular
T5232 4070-4090 CHEBI:28680 denotes cytosine arabinoside
T5231 4041-4046 CL_GO_EXT:cell denotes cells
T5230 4016-4019 CHEBI:16526 denotes CO2
T5229 3949-3954 GO:0040007 denotes grown
T5228 3935-3943 UBERON:0001851 denotes cortices
T5227 3929-3934 NCBITaxon:10088 denotes mouse
T5226 3921-3928 PATO_UBERON_EXT:neonate_or_newborn denotes newborn
T5225 3884-3903 CL:0002605 denotes Cortical astrocytes
T5224 3884-3892 UBERON:0001851 denotes Cortical
T5223 3879-3882 CHEBI:16526 denotes CO2
T5222 3812-3819 CL:0000540 denotes neurons
T5221 3759-3778 CL:0002605 denotes cortical astrocytes
T5220 3759-3767 UBERON:0001851 denotes cortical
T5219 3753-3758 NCBITaxon:10088 denotes mouse
T5218 3664-3683 CL:0002608 denotes hippocampal neurons
T5217 3664-3675 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes hippocampal
T5216 3619-3626 PR_EXT:000027795 denotes trypsin
T5215 3475-3482 UBERON:0000479 denotes tissues
T5214 3442-3449 UBERON:0000922 denotes embryos
T5213 3436-3441 NCBITaxon:10088 denotes mouse
T5212 3398-3411 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes hippocampuses
T5211 3314-3332 CL:0002608 denotes Hippocampal neuron
T5210 3314-3325 UBERON_EXT:hippocampus_proper_or_hippocampal_formation denotes Hippocampal
T5209 3277-3281 CL_GO_EXT:cell denotes cell
T5208 3268-3281 CL:0000540 denotes Neuronal cell
T5022 3104-3111 CHEBI_PR_EXT:protein denotes protein
T5021 3077-3094 SO_EXT:0000110 denotes sequence features
T5020 3018-3023 SO_EXT:sequence_or_structure_motif denotes motif
T5019 3009-3017 SO_EXT:biological_sequence denotes sequence
T5018 2924-2932 SO_EXT:biological_sequence denotes sequence
T5017 2863-2871 SO_EXT:biological_sequence denotes sequence
T5016 2767-2775 SO:0000857 denotes homology
T5015 2763-2766 CHEBI_SO_EXT:DNA denotes DNA
T5014 2751-2758 CHEBI_PR_EXT:protein denotes Protein
T5013 2733-2737 SO_EXT:0000704 denotes Gene
T5012 2685-2693 SO_EXT:sequence_assembly_entity_or_process denotes assembly
T5011 2676-2684 SO_EXT:biological_sequence denotes Sequence
T5010 2658-2666 SO_EXT:biological_sequence denotes Sequence
T4940 2611-2616 NCBITaxon:10088 denotes Mouse
T4939 2553-2559 SO_EXT:0001026 denotes genome
T4938 2547-2552 NCBITaxon:10088 denotes mouse
T4937 2523-2529 SO_EXT:sequence_cloned_entity denotes clones
T4936 2465-2469 PR_EXT:ACDP_or_CNNM denotes Acdp
T4935 2450-2456 SO_EXT:0000205 denotes 3' UTR
T4934 2437-2444 SO_EXT:0000112 denotes Primers
T4933 2396-2415 GO_SO_EXT:chromosomal_part_or_position_or_region_or_site denotes chromosome location
T4932 2367-2375 OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process denotes Genetics
T4931 2324-2329 NCBITaxon:10088 denotes mouse
T4930 2303-2315 GO_PATO_EXT:biological_localization_process_or_quality denotes localization
T4929 2292-2302 GO_SO_EXT:chromosome denotes Chromosome
T4814 1895-1904 CHEBI_SO_EXT:N_terminus_or_N_terminal_region denotes terminals
T4813 1886-1888 _FRAGMENT denotes N-
T4812 1880-1885 PR_EXT:000005643 denotes Acdp1
T4811 1856-1862 NCBITaxon:6462 denotes limpet
T4810 1833-1839 MOP:0000779 denotes linked
T4809 1833-1839 SO_EXT:sequence_fusion_process denotes linked
T4808 1824-1832 CHEBI_SO_EXT:peptide_or_peptide_region denotes Peptides
T4807 1778-1797 GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production denotes Antibody production
T4806 1778-1786 GO:0042571 denotes Antibody
T4646 1678-1681 CHEBI:8984 denotes SDS
T4645 1668-1671 CHEBI_EXT:saline_sodium_citrate denotes SSC
T4644 1652-1658 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T4643 1592-1595 CHEBI:8984 denotes SDS
T4642 1582-1585 CHEBI_EXT:saline_sodium_citrate denotes SSC
T4641 1570-1576 CHEBI_CHMO_EXT:buffer_solution denotes buffer
T4640 1484-1498 GO:0097617 denotes Hybridizations
T4639 1439-1445 SO_EXT:0000112 denotes primer
T4638 1417-1421 CHEBI_EXT:dCTP denotes dCTP
T4637 1413-1416 CHEBI:37972 denotes 32P
T4636 1398-1405 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T4635 1380-1390 GO:0006412 denotes translated
T4634 1375-1397 SO_EXT:0000205 denotes 3' untranslated region
T4633 1362-1366 SO_EXT:0000147 denotes exon
T4632 1348-1350 SO_EXT:0000028 denotes bp
T4631 1311-1315 SO_EXT:0000704 denotes gene
T4630 1306-1310 PR_EXT:ACDP_or_CNNM denotes Acdp
T4629 1236-1243 UBERON:0000479 denotes tissues
T4628 1230-1235 NCBITaxon:10088 denotes mouse
T4822 2211-2221 GO:0042571 denotes antibodies
T4821 2006-2010 PR_EXT:ACDP_or_CNNM denotes Acdp
T4820 1992-1997 PR_EXT:000005643 denotes Acdp1
T4819 1964-1974 GO:0042571 denotes antibodies
T4818 1950-1974 GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production denotes generation of antibodies
T4817 1923-1929 SO_EXT:0000417 denotes domain
T4816 1913-1922 SO_EXT:biological_conservation_process_or_quality denotes conserved
T4815 1893-1904 CHEBI_SO_EXT:C_terminus_or_C_terminal_region denotes C-terminals
T4391 1128-1131 CHEBI_SO_EXT:DNA denotes DNA
T4390 1124-1127 SO_EXT:0000153 denotes BAC
T4389 1097-1106 SO_EXT:biological_sequence denotes sequences
T4388 1090-1096 SO_EXT:0000204 denotes 5' UTR
T4387 1075-1079 SO_EXT:0000147 denotes exon
T4386 1063-1071 SO_EXT:biological_sequence denotes sequence
T4385 1021-1037 SO_EXT:coding_sequence denotes coding sequences
T4384 994-1002 SO_EXT:biological_sequence denotes sequence
T4383 968-982 SO_EXT:0000132 denotes reverse primer
T4382 953-961 SO_EXT:biological_sequence denotes sequence
T4381 916-930 SO_EXT:0000121 denotes forward primer
T4380 877-885 SO_EXT:biological_sequence denotes sequence
T4379 813-820 CHEBI:2511 denotes agarose
T4378 773-785 SO_EXT:0000006 denotes PCR products
T4377 730-739 GO:0097617 denotes annealing
T4376 709-721 SO_EXT:0000006 denotes PCR products
T4375 683-687 SO_EXT:0000704 denotes gene
T4374 677-682 NCBITaxon:10088 denotes mouse
T4373 617-631 SO_EXT:0000121 denotes forward primer
T4372 611-616 NCBITaxon:9606 denotes human
T4371 589-597 SO_EXT:biological_sequence denotes sequence
T4370 585-588 SO_EXT:0000345 denotes EST
T4369 579-584 NCBITaxon:10088 denotes mouse
T4368 555-569 SO_EXT:0000132 denotes reverse primer
T4367 493-502 GO:0097617 denotes annealing
T4366 476-480 SO_EXT:cDNA denotes cDNA
T4365 470-475 NCBITaxon:10088 denotes mouse
T4364 456-464 SO_EXT:biological_sequence denotes sequence
T4363 446-455 SO_EXT:0000853 denotes homologue
T4362 414-420 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes marker
T4361 410-413 SO_EXT:0000345 denotes EST
T4360 404-409 NCBITaxon:10088 denotes mouse
T4359 380-394 SO_EXT:0000132 denotes reverse primer
T4358 374-379 NCBITaxon:10088 denotes mouse
T4357 355-366 SO_EXT:0000318 denotes start codon
T4356 334-347 SO_EXT:coding_sequence denotes coding region
T4355 329-333 SO_EXT:cDNA denotes cDNA
T4354 321-325 PR_EXT:ACDP_or_CNNM denotes ACDP
T4353 315-320 NCBITaxon:9606 denotes human
T4352 289-303 SO_EXT:0000121 denotes forward primer
T4351 274-278 PR_EXT:ACDP_or_CNNM denotes Acdp
T4350 244-251 CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag denotes markers
T4349 240-243 SO_EXT:0000345 denotes EST
T4348 223-226 SO_EXT:0000345 denotes EST
T4347 217-222 NCBITaxon:10088 denotes mouse
T4346 183-192 SO_EXT:biological_sequence denotes sequences
T4345 180-182 CHEBI_SO_EXT:amino_acid denotes AA
T4344 160-165 SO_EXT:cDNA denotes cDNAs
T4343 155-159 PR_EXT:ACDP_or_CNNM denotes ACDP
T4342 149-154 NCBITaxon:9606 denotes human
T4341 98-107 SO_EXT:biological_sequence denotes sequences
T4340 88-97 SO_EXT:0000853 denotes homologue
T4339 82-87 NCBITaxon:9606 denotes human
T4338 47-51 SO_EXT:0000704 denotes gene
T4337 42-46 PR_EXT:ACDP_or_CNNM denotes Acdp
T4336 27-34 SO_EXT:sequence_cloning_process denotes cloning
T4335 22-26 SO_EXT:cDNA denotes cDNA
T4334 14-21 SO_EXT:sequence_cloning_process denotes cloning
T4333 9-13 SO_EXT:cDNA denotes cDNA
R2903 T4814 T4813 _lexicallyChainedTo terminals,N-