PMC:340383 / 17882-23326 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T5718	5442-5443	-RRB-	denotes	)
T5717	5437-5442	NN	denotes	Acdp4
T5716	5436-5437	-LRB-	denotes	(
T5715	5427-5435	NN	denotes	AF216963
T5714	5423-5426	CC	denotes	and
T5713	5421-5422	-RRB-	denotes	)
T5701	5379-5380	-LRB-	denotes	(
T5700	5362-5369	NNS	denotes	numbers
T5699	5370-5378	NN	denotes	AF202994
T5698	5352-5361	NN	denotes	accession
T5697	5347-5351	IN	denotes	with
T5696	5342-5346	NN	denotes	bank
T5695	5337-5341	NN	denotes	gene
T5694	5334-5336	IN	denotes	in
T5693	5319-5323	VBN	denotes	been
T5692	5311-5318	RB	denotes	already
T5691	5306-5310	VBP	denotes	have
T5690	5294-5298	NN	denotes	gene
T5689	5289-5293	NN	denotes	Acdp
T5688	5299-5305	NN	denotes	family
T5687	5285-5288	DT	denotes	the
T5686	5281-5284	IN	denotes	for
T5685	5324-5333	VBN	denotes	deposited
T5684	5266-5270	NN	denotes	cDNA
T5683	5271-5280	NNS	denotes	sequences
T5682	5262-5265	DT	denotes	The
T5681	5261-5444	sentence	denotes	The cDNA sequences for the Acdp gene family have already been deposited in gene bank with accession numbers AF202994 (Acdp1), AF216961 (Acdp2), AF216964 (Acdp3) and AF216963 (Acdp4).
T5680	5245-5254	NN	denotes	accession
T5678	5240-5244	NN	denotes	bank
T5677	5235-5239	NN	denotes	Gene
T5370	3860-3870	NN	denotes	atmosphere
T5369	3847-3848	DT	denotes	a
T5368	3844-3846	IN	denotes	in
T5367	3841-3843	NN	denotes	°C
T5366	3839-3841	CD	denotes	37
T5365	3836-3838	IN	denotes	at
T5364	3820-3824	VBD	denotes	were
T5363	3825-3835	VBN	denotes	maintained
T5362	3812-3819	NNS	denotes	neurons
T5361	3808-3811	DT	denotes	The
T5360	3807-3883	sentence	denotes	The neurons were maintained at 37°C in a humidified atmosphere with 5% CO2.
T5359	3806-3807	.	denotes	.
T5358	3801-3806	RB	denotes	below
T5357	3791-3800	VBN	denotes	described
T5356	3788-3790	IN	denotes	as
T5355	3779-3787	VBN	denotes	obtained
T5354	3759-3767	JJ	denotes	cortical
T5353	3768-3778	NNS	denotes	astrocytes
T5352	3753-3758	NN	denotes	mouse
T5351	3750-3752	IN	denotes	of
T5350	3730-3739	JJ	denotes	confluent
T5349	3740-3749	NN	denotes	monolayer
T5348	3728-3729	DT	denotes	a
T5347	3723-3727	IN	denotes	with
T5346	3716-3722	VBN	denotes	coated
T5345	3705-3715	NNS	denotes	coverslips
T5344	3699-3704	NN	denotes	glass
T5343	3696-3698	IN	denotes	on
T5342	3684-3688	VBD	denotes	were
T5341	3689-3695	VBN	denotes	plated
T5340	3664-3675	JJ	denotes	hippocampal
T5339	3652-3663	JJ	denotes	dissociated
T5338	3676-3683	NNS	denotes	neurons
T5337	3648-3651	DT	denotes	The
T5336	3647-3807	sentence	denotes	The dissociated hippocampal neurons were plated on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes obtained as described below.
T5335	3646-3647	.	denotes	.
T5334	3645-3646	-RRB-	denotes	)
T5333	3628-3632	NNP	denotes	Life
T5332	3633-3645	NNP	denotes	Technologies
T5331	3627-3628	-LRB-	denotes	(
T5330	3619-3626	NN	denotes	trypsin
T5329	3617-3618	NN	denotes	%
T5328	3613-3617	CD	denotes	0.25
T5327	3608-3612	CC	denotes	plus
T5326	3606-3607	-RRB-	denotes	)
T5325	3589-3593	NNP	denotes	Life
T5324	3594-3606	NNP	denotes	Technologies
T5323	3588-3589	-LRB-	denotes	(
T5322	3580-3587	NN	denotes	culture
T5321	3569-3579	NN	denotes	suspension
T5320	3565-3568	IN	denotes	for
T5319	3556-3564	VBN	denotes	modified
T5318	3554-3555	-RRB-	denotes	)
T5317	3538-3547	JJ	denotes	essential
T5316	3548-3554	NN	denotes	medium
T5315	3530-3537	JJ	denotes	minimum
T5314	3529-3530	-LRB-	denotes	(
T5313	3525-3528	NN	denotes	MEM
T5312	3522-3524	IN	denotes	in
T5311	3519-3521	NN	denotes	°C
T5310	3517-3519	CD	denotes	37
T5309	3514-3516	IN	denotes	at
T5308	3510-3513	NN	denotes	min
T5307	3507-3509	CD	denotes	20
T5306	3503-3506	IN	denotes	for
T5305	3488-3492	RB	denotes	then
T5304	3483-3487	VBD	denotes	were
T5303	3493-3502	VBN	denotes	incubated
T5302	3475-3482	NNS	denotes	tissues
T5301	3471-3474	DT	denotes	The
T5300	3470-3647	sentence	denotes	The tissues were then incubated for 20 min at 37°C in MEM (minimum essential medium) modified for suspension culture (Life Technologies) plus 0.25% trypsin (Life Technologies).
T5299	3469-3470	.	denotes	.
T5298	3464-3469	FW	denotes	utero
T5297	3461-3463	FW	denotes	in
T5296	3456-3460	NNS	denotes	days
T5295	3453-3455	CD	denotes	16
T5294	3450-3452	IN	denotes	at
T5293	3442-3449	NNS	denotes	embryos
T5292	3436-3441	NN	denotes	mouse
T5291	3431-3435	IN	denotes	from
T5290	3427-3430	RP	denotes	out
T5289	3412-3416	VBD	denotes	were
T5288	3398-3411	NNS	denotes	hippocampuses
T5287	3394-3397	DT	denotes	the
T5286	3392-3394	,	denotes	,
T5285	3387-3392	NN	denotes	brief
T5284	3417-3426	VBN	denotes	dissected
T5283	3384-3386	IN	denotes	In
T5282	3383-3470	sentence	denotes	In brief, the hippocampuses were dissected out from mouse embryos at 16 days in utero.
T5281	3382-3383	.	denotes	.
T5280	3381-3382	-RRB-	denotes	]
T5279	3380-3381	CD	denotes	6
T5278	3379-3380	-LRB-	denotes	[
T5277	3359-3369	RB	denotes	previously
T5276	3370-3378	VBN	denotes	reported
T5275	3356-3358	IN	denotes	as
T5274	3342-3346	VBD	denotes	were
T5273	3347-3355	VBN	denotes	prepared
T5272	3326-3332	NN	denotes	neuron
T5271	3333-3341	NNS	denotes	cultures
T5270	3314-3325	JJ	denotes	Hippocampal
T5269	3313-3383	sentence	denotes	Hippocampal neuron cultures were prepared as previously reported [6].
T5268	3298-3313	NN	denotes	immunostanining
T5267	3294-3297	CC	denotes	and
T5266	3277-3281	NN	denotes	cell
T5265	3282-3293	NN	denotes	preparation
T5264	3268-3276	JJ	denotes	Neuronal
T5100	3126-3130	NN	denotes	tree
T5099	3113-3125	JJ	denotes	Phylogenetic
T5098	3112-3266	sentence	denotes	Phylogenetic tree was constructed by Clustalw program (version 1.81) using UPGMA (Unweighted Pair Group Method using Arithmetic averages) algorithm [14].
T5097	3111-3112	.	denotes	.
T5096	3104-3111	NN	denotes	protein
T5095	3098-3103	JJ	denotes	known
T5094	3095-3097	IN	denotes	of
T5093	3086-3094	NNS	denotes	features
T5092	3077-3085	NN	denotes	sequence
T5091	3067-3076	VBG	denotes	searching
T5090	3063-3066	IN	denotes	for
T5089	3053-3057	VBD	denotes	were
T5088	3045-3051	NN	denotes	3Dpssm
T5087	3041-3044	CC	denotes	and
T5086	3033-3039	NN	denotes	ExPASy
T5085	3031-3033	,	denotes	,
T5084	3018-3023	NN	denotes	motif
T5083	3024-3030	NN	denotes	search
T5082	3009-3017	NN	denotes	sequence
T5081	3007-3009	,	denotes	,
T5080	2995-3006	NN	denotes	ProfileScan
T5079	2993-2995	,	denotes	,
T5078	2977-2983	NN	denotes	Search
T5077	2984-2992	NN	denotes	Launcher
T5076	2973-2976	NN	denotes	BCM
T5075	2963-2972	VBG	denotes	including
T5074	3058-3062	VBN	denotes	used
T5073	2954-2962	NNS	denotes	programs
T5072	2945-2953	JJ	denotes	Multiple
T5071	2944-3112	sentence	denotes	Multiple programs including BCM Search Launcher , ProfileScan , sequence motif search , ExPASy and 3Dpssm were used for searching sequence features of known protein.
T5070	2943-2944	.	denotes	.
T5069	2924-2932	NN	denotes	sequence
T5068	2915-2923	JJ	denotes	pairwise
T5067	2911-2914	CC	denotes	and
T5066	2933-2942	NN	denotes	alignment
T5065	2903-2910	NN	denotes	GeneDoc
T5064	2898-2902	IN	denotes	with
T5063	2883-2887	VBD	denotes	were
T5062	2888-2897	VBN	denotes	performed
T5061	2863-2871	NN	denotes	sequence
T5060	2872-2882	NNS	denotes	alignments
T5059	2854-2862	JJ	denotes	Multiple
T5058	2853-2944	sentence	denotes	Multiple sequence alignments were performed with GeneDoc and pairwise sequence alignment .
T5057	2852-2853	.	denotes	.
T5056	2836-2842	NN	denotes	blastn
T5055	2832-2835	CC	denotes	and
T5054	2825-2831	NN	denotes	blastp
T5053	2823-2825	,	denotes	,
T5052	2816-2823	NN	denotes	tblastx
T5051	2814-2816	,	denotes	,
T5050	2843-2851	NNS	denotes	programs
T5049	2807-2814	NN	denotes	tblastn
T5048	2802-2806	IN	denotes	with
T5047	2798-2801	RP	denotes	out
T5046	2785-2789	VBD	denotes	were
T5045	2790-2797	VBN	denotes	carried
T5044	2776-2784	NNS	denotes	searches
T5043	2763-2766	NN	denotes	DNA
T5042	2759-2762	CC	denotes	and
T5041	2767-2775	NN	denotes	homology
T5040	2751-2758	NN	denotes	Protein
T5039	2750-2853	sentence	denotes	Protein and DNA homology searches were carried out with tblastn, tblastx, blastp and blastn programs .
T5038	2749-2750	.	denotes	.
T5037	2748-2749	-RRB-	denotes	)
T5036	2738-2743	NNPS	denotes	Codes
T5035	2733-2737	NNP	denotes	Gene
T5034	2744-2748	NNP	denotes	Corp
T5033	2732-2733	-LRB-	denotes	(
T5032	2721-2731	NN	denotes	Sequencher
T5031	2713-2720	NN	denotes	program
T5030	2708-2712	IN	denotes	with
T5029	2694-2697	VBD	denotes	was
T5028	2698-2707	VBN	denotes	performed
T5027	2685-2693	NN	denotes	assembly
T5026	2676-2684	NN	denotes	Sequence
T5025	2675-2750	sentence	denotes	Sequence assembly was performed with program Sequencher (Gene Codes Corp).
T5024	2667-2675	NNS	denotes	analyses
T5023	2658-2666	NN	denotes	Sequence
T5004	2655-2656	.	denotes	.
T5003	2647-2655	NN	denotes	analysis
T5002	2643-2646	IN	denotes	for
T5001	2617-2626	NNP	denotes	Radiation
T5000	2627-2633	NNP	denotes	Hybrid
T4999	2611-2616	NNP	denotes	Mouse
T4998	2600-2610	NNP	denotes	Laboratory
T4997	2592-2599	NNP	denotes	Jackson
T4996	2634-2642	NNP	denotes	Database
T4995	2588-2591	DT	denotes	the
T4994	2585-2587	IN	denotes	to
T4993	2570-2574	VBD	denotes	were
T4992	2575-2584	VBN	denotes	submitted
T4991	2565-2569	NNS	denotes	data
T4990	2561-2564	DT	denotes	The
T4989	2560-2656	sentence	denotes	The data were submitted to the Jackson Laboratory Mouse Radiation Hybrid Database for analysis.
T4988	2559-2560	.	denotes	.
T4987	2547-2552	NN	denotes	mouse
T4986	2553-2559	NN	denotes	genome
T4985	2543-2546	DT	denotes	the
T4984	2530-2542	VBG	denotes	representing
T4983	2516-2522	JJ	denotes	hybrid
T4982	2506-2515	NN	denotes	radiation
T4981	2502-2505	CD	denotes	100
T4980	2523-2529	NNS	denotes	clones
T4979	2498-2501	DT	denotes	the
T4978	2490-2497	VB	denotes	amplify
T4977	2487-2489	TO	denotes	to
T4976	2477-2481	VBD	denotes	were
T4975	2465-2469	NN	denotes	Acdp
T4974	2470-2476	NN	denotes	member
T4973	2460-2464	DT	denotes	each
T4972	2457-2459	IN	denotes	of
T5655	5232-5233	.	denotes	.
T5654	5231-5232	-RRB-	denotes	)
T5653	5229-5231	NNP	denotes	MD
T5652	5227-5229	,	denotes	,
T5651	5218-5227	NNP	denotes	Frederick
T5650	5216-5218	,	denotes	,
T5649	5199-5204	NNP	denotes	Scion
T5648	5205-5216	NNP	denotes	Corporation
T5647	5198-5199	-LRB-	denotes	(
T5646	5183-5188	NN	denotes	Image
T5645	5177-5182	NNP	denotes	Scion
T5644	5189-5197	NN	denotes	software
T5643	5173-5176	DT	denotes	the
T5642	5170-5172	IN	denotes	by
T5641	5161-5169	VBN	denotes	acquired
T5640	5157-5160	CC	denotes	and
T5639	5146-5149	NN	denotes	CCD
T5638	5150-5156	NN	denotes	camera
T5637	5144-5145	DT	denotes	a
T5636	5139-5143	IN	denotes	with
T5635	5125-5129	VBD	denotes	were
T5634	5130-5138	VBN	denotes	captured
T5633	5118-5124	NNS	denotes	Images
T5632	5117-5233	sentence	denotes	Images were captured with a CCD camera and acquired by the Scion Image software (Scion Corporation, Frederick, MD).
T5631	5116-5117	.	denotes	.
T5630	5115-5116	-RRB-	denotes	)
T5629	5105-5109	NNP	denotes	Carl
T5628	5110-5115	NNP	denotes	Zeiss
T5627	5104-5105	-LRB-	denotes	(
T5560	4766-4768	DT	denotes	an
T5559	4761-4765	IN	denotes	with
T5558	4746-4750	VBD	denotes	were
T5557	4740-4745	NNS	denotes	cells
T5556	4736-4739	DT	denotes	the
T5555	4734-4736	,	denotes	,
T5554	4722-4725	NN	denotes	PBS
T5553	4726-4734	NN	denotes	solution
T5552	4711-4715	NN	denotes	goat
T5551	4716-4721	NN	denotes	serum
T5550	4709-4710	NN	denotes	%
T5549	4708-4709	CD	denotes	1
T5548	4703-4707	IN	denotes	with
T5547	4695-4702	NN	denotes	washing
T5546	4685-4694	JJ	denotes	extensive
T5545	4751-4760	VBN	denotes	incubated
T5544	4679-4684	IN	denotes	After
T5543	4678-4894	sentence	denotes	After extensive washing with 1% goat serum PBS solution, the cells were incubated with an Alex 488 conjugated secondary antibody (1:100 in 1% goat serum PBS solution, Molecular Probes) for 3 hrs at room temperature.
T5542	4677-4678	.	denotes	.
T5541	4675-4677	NN	denotes	°C
T5540	4674-4675	CD	denotes	4
T5539	4671-4673	IN	denotes	at
T5538	4661-4670	RB	denotes	overnight
T5537	4659-4660	-RRB-	denotes	)
T5536	4655-4659	CD	denotes	3000
T5535	4654-4655	SYM	denotes	:
T5534	4653-4654	CD	denotes	1
T5533	4652-4653	-LRB-	denotes	(
T5532	4633-4642	JJ	denotes	anti-ACDP
T5531	4622-4632	JJ	denotes	polyclonal
T5530	4615-4621	NN	denotes	rabbit
T5529	4643-4651	NN	denotes	antibody
T5528	4613-4614	DT	denotes	a
T5527	4608-4612	IN	denotes	with
T5526	4598-4607	VBN	denotes	incubated
T5525	4585-4597	RB	denotes	subsequently
T5524	4581-4584	CC	denotes	and
T5523	4579-4581	,	denotes	,
T5522	4569-4573	NN	denotes	goat
T5521	4562-4568	JJ	denotes	normal
T5520	4559-4561	CD	denotes	30
T5519	4558-4559	SYM	denotes	:
T5518	4574-4579	NN	denotes	serum
T5517	4557-4558	CD	denotes	1
T5516	4546-4556	VBG	denotes	containing
T5515	4528-4536	VBG	denotes	blocking
T5514	4537-4545	NN	denotes	solution
T5513	4526-4527	DT	denotes	a
T5512	4521-4525	IN	denotes	with
T5511	4506-4510	VBD	denotes	were
T5510	4500-4505	NNS	denotes	cells
T5509	4496-4499	DT	denotes	the
T5508	4494-4496	,	denotes	,
T5507	4489-4494	NNS	denotes	times
T5506	4483-4488	CD	denotes	three
T5505	4479-4482	NN	denotes	PBS
T5504	4474-4478	IN	denotes	with
T5503	4466-4473	VBG	denotes	washing
T5502	4511-4520	VBN	denotes	incubated
T5501	4460-4465	IN	denotes	After
T5500	4459-4678	sentence	denotes	After washing with PBS three times, the cells were incubated with a blocking solution containing 1:30 normal goat serum, and subsequently incubated with a rabbit polyclonal anti-ACDP antibody (1:3000) overnight at 4°C.
T5499	4458-4459	.	denotes	.
T5498	4456-4458	NN	denotes	hr
T5497	4454-4455	CD	denotes	1
T5496	4450-4453	IN	denotes	for
T5495	4447-4449	NN	denotes	°C
T5494	4446-4447	CD	denotes	4
T5493	4443-4445	IN	denotes	at
T5492	4438-4439	HYPH	denotes	-
T5491	4437-4438	NN	denotes	X
T5490	4430-4436	NN	denotes	Triton
T5489	4439-4442	NN	denotes	100
T5488	4428-4429	NN	denotes	%
T5487	4425-4428	CD	denotes	0.4
T5486	4421-4424	CC	denotes	and
T5485	4417-4420	NN	denotes	PFA
T5484	4415-4416	NN	denotes	%
T5483	4414-4415	CD	denotes	4
T5482	4403-4413	VBG	denotes	containing
T5481	4394-4402	NN	denotes	solution
T5480	4392-4393	DT	denotes	a
T5479	4389-4391	IN	denotes	in
T5478	4379-4388	VBN	denotes	incubated
T5477	4374-4378	RB	denotes	then
T5476	4370-4373	CC	denotes	and
T5475	4367-4369	NN	denotes	°C
T5474	4366-4367	CD	denotes	4
T5473	4363-4365	IN	denotes	at
T5472	4359-4362	NNS	denotes	hrs
T5471	4356-4358	CD	denotes	12
T5470	4352-4355	IN	denotes	for
T5469	4350-4351	-RRB-	denotes	)
T5468	4347-4350	NN	denotes	PFA
T5467	4346-4347	-LRB-	denotes	(
T5466	4329-4345	NN	denotes	paraformaldehyde
T5465	4327-4328	NN	denotes	%
T5464	4326-4327	CD	denotes	4
T5463	4315-4325	VBG	denotes	containing
T5462	4311-4314	NN	denotes	PBS
T5461	4308-4310	IN	denotes	in
T5460	4296-4301	RB	denotes	first
T5459	4291-4295	VBD	denotes	were
T5458	4280-4290	NNS	denotes	coverslips
T5457	4276-4279	DT	denotes	the
T5456	4273-4275	IN	denotes	on
T5455	4267-4272	NNS	denotes	cells
T5454	4258-4266	JJ	denotes	neuronal
T5453	4256-4258	,	denotes	,
T5452	4242-4256	NN	denotes	immunostaining
T5451	4302-4307	VBN	denotes	fixed
T5450	4238-4241	IN	denotes	For
T5449	4237-4459	sentence	denotes	For immunostaining, neuronal cells on the coverslips were first fixed in PBS containing 4% paraformaldehyde (PFA) for 12 hrs at 4°C and then incubated in a solution containing 4% PFA and 0.4% Triton X-100 at 4°C for 1 hr.
T5448	4236-4237	.	denotes	.
T5447	4226-4236	NNS	denotes	coverslips
T5446	4218-4225	VBG	denotes	coating
T5445	4214-4217	IN	denotes	for
T5444	4204-4208	VBD	denotes	were
T5443	4198-4203	NNS	denotes	cells
T5442	4194-4197	DT	denotes	the
T5441	4192-4194	,	denotes	,
T5440	4186-4192	NN	denotes	debris
T5439	4177-4185	JJ	denotes	cellular
T5438	4172-4176	IN	denotes	with
T5437	4166-4171	NNS	denotes	media
T5436	4162-4165	DT	denotes	the
T5435	4159-4161	IN	denotes	of
T5434	4152-4158	NN	denotes	remove
T5433	4209-4213	VBN	denotes	used
T5432	4146-4151	IN	denotes	After
T5431	4145-4237	sentence	denotes	After remove of the media with cellular debris, the cells were used for coating coverslips.
T5430	4144-4145	.	denotes	.
T5429	4142-4144	NN	denotes	°C
T5428	4140-4142	CD	denotes	37
T5427	4137-4139	IN	denotes	at
T5426	4129-4130	SYM	denotes	–
T5425	4130-4132	CD	denotes	24
T5424	4127-4129	CD	denotes	12
T5423	4133-4136	NNS	denotes	hrs
T5422	4116-4126	JJ	denotes	additional
T5421	4112-4115	IN	denotes	for
T5420	4103-4111	VBN	denotes	cultured
T5419	4099-4102	CC	denotes	and
T5418	4097-4098	-RRB-	denotes	)
T5417	4092-4097	NNP	denotes	Sigma
T5416	4091-4092	-LRB-	denotes	(
T5415	4070-4078	NN	denotes	cytosine
T5414	4079-4090	NN	denotes	arabinoside
T5413	4068-4069	NN	denotes	M
T5412	4065-4066	SYM	denotes	-
T5411	4066-4067	CD	denotes	5
T5410	4063-4065	CD	denotes	10
T5409	4060-4062	IN	denotes	to
T5408	4047-4051	VBD	denotes	were
T5407	4052-4059	VBN	denotes	exposed
T5406	4041-4046	NNS	denotes	cells
T5405	4037-4040	DT	denotes	The
T5404	4036-4145	sentence	denotes	The cells were exposed to 10-5 M cytosine arabinoside (Sigma) and cultured for additional 12–24 hrs at 37°C.
T5403	4035-4036	.	denotes	.
T5402	4026-4035	JJ	denotes	confluent
T5401	4020-4025	IN	denotes	until
T5400	4016-4019	NN	denotes	CO2
T5399	4014-4015	NN	denotes	%
T5398	4013-4014	CD	denotes	5
T5397	4008-4012	IN	denotes	with
T5396	3986-3996	JJ	denotes	humidified
T5395	3997-4007	NN	denotes	atmosphere
T5394	3984-3985	DT	denotes	a
T5393	3981-3983	IN	denotes	in
T5392	3978-3980	NN	denotes	°C
T5391	3976-3978	CD	denotes	37
T5390	3973-3975	IN	denotes	at
T5389	3966-3972	NNS	denotes	flasks
T5388	3958-3965	NN	denotes	culture
T5387	3955-3957	IN	denotes	in
T5386	3944-3948	VBD	denotes	were
T5385	3935-3943	NNS	denotes	cortices
T5384	3929-3934	NN	denotes	mouse
T5383	3921-3928	JJ	denotes	newborn
T5382	3916-3920	IN	denotes	from
T5381	3904-3915	VBN	denotes	dissociated
T5380	3949-3954	VBN	denotes	grown
T5379	3893-3903	NNS	denotes	astrocytes
T5378	3884-3892	JJ	denotes	Cortical
T5377	3883-4036	sentence	denotes	Cortical astrocytes dissociated from newborn mouse cortices were grown in culture flasks at 37°C in a humidified atmosphere with 5% CO2 until confluent.
T5376	3882-3883	.	denotes	.
T5375	3879-3882	NN	denotes	CO2
T5374	3877-3878	NN	denotes	%
T5373	3876-3877	CD	denotes	5
T5372	3871-3875	IN	denotes	with
T5371	3849-3859	JJ	denotes	humidified
T4971	2451-2452	SYM	denotes	'
T4970	2453-2456	NN	denotes	UTR
T4969	2450-2451	CD	denotes	3
T4968	2445-2449	IN	denotes	from
T4967	2482-2486	VBN	denotes	used
T4966	2437-2444	NNS	denotes	Primers
T4965	2436-2560	sentence	denotes	Primers from 3' UTR of each Acdp member were used to amplify the 100 radiation hybrid clones representing the mouse genome.
T4964	2435-2436	.	denotes	.
T4963	2424-2428	NN	denotes	Acdp
T4962	2429-2435	NN	denotes	member
T4961	2419-2423	DT	denotes	each
T4960	2416-2418	IN	denotes	of
T4959	2396-2406	NN	denotes	chromosome
T4958	2407-2415	NN	denotes	location
T4957	2392-2395	DT	denotes	the
T4956	2388-2391	VB	denotes	map
T4955	2385-2387	TO	denotes	to
T4954	2376-2379	VBD	denotes	was
T4953	2367-2375	NNP	denotes	Genetics
T4952	2358-2366	NNP	denotes	Research
T4951	2353-2357	IN	denotes	from
T4950	2380-2384	VBN	denotes	used
T4949	2340-2346	NN	denotes	hybrid
T4948	2330-2339	NN	denotes	radiation
T4947	2324-2329	NN	denotes	mouse
T4946	2320-2323	NN	denotes	T31
T4945	2347-2352	NN	denotes	panel
T4944	2316-2319	DT	denotes	The
T4943	2315-2436	sentence	denotes	The T31 mouse radiation hybrid panel from Research Genetics was used to map the chromosome location of each Acdp member.
T4942	2303-2315	NN	denotes	localization
T4941	2292-2302	NN	denotes	Chromosome
T4917	2289-2290	.	denotes	.
T4916	2276-2284	NN	denotes	exposure
T4915	2285-2289	NN	denotes	time
T4914	2268-2275	JJ	denotes	optimal
T4913	2263-2267	IN	denotes	with
T4912	2257-2262	NNS	denotes	films
T4911	2251-2256	NN	denotes	X-ray
T4910	2246-2250	IN	denotes	with
T4909	2231-2235	RB	denotes	then
T4908	2236-2245	VBN	denotes	developed
T4907	2226-2230	VBD	denotes	were
T4906	2222-2225	CC	denotes	and
T4905	2201-2210	JJ	denotes	secondary
T4904	2197-2200	CC	denotes	and
T4903	2211-2221	NNS	denotes	antibodies
T4902	2189-2196	JJ	denotes	primary
T4901	2184-2188	IN	denotes	with
T4900	2173-2183	NN	denotes	incubation
T4899	2167-2172	IN	denotes	after
T4898	2155-2166	RB	denotes	extensively
T4897	2143-2147	VBD	denotes	were
T4896	2148-2154	VBN	denotes	washed
T4895	2133-2142	NNS	denotes	membranes
T4894	2129-2132	DT	denotes	The
T4893	2128-2290	sentence	denotes	The membranes were washed extensively after incubation with primary and secondary antibodies and were then developed with X-ray films with optimal exposure time.
T4892	2127-2128	.	denotes	.
T4891	2126-2127	-RRB-	denotes	]
T4890	2125-2126	CD	denotes	1
T4889	2124-2125	-LRB-	denotes	[
T4888	2113-2123	RB	denotes	previously
T4887	2103-2112	VBN	denotes	described
T4886	2100-2102	IN	denotes	as
T4885	2098-2099	-RRB-	denotes	)
T4786	1775-1776	.	denotes	.
T4785	1774-1775	-RRB-	denotes	]
T4784	1772-1773	,	denotes	,
T4783	1771-1772	CD	denotes	3
T4782	1773-1774	CD	denotes	4
T4781	1770-1771	-LRB-	denotes	[
T4780	1763-1769	RBR	denotes	longer
T4779	1760-1762	CC	denotes	or
T4778	1750-1759	NN	denotes	overnight
T4777	1746-1749	IN	denotes	for
T4776	1740-1745	NNS	denotes	films
T4775	1734-1739	NN	denotes	X-ray
T4774	1731-1733	IN	denotes	to
T4773	1718-1722	VBD	denotes	were
T4772	1723-1730	VBN	denotes	exposed
T4771	1710-1717	NNS	denotes	filters
T4770	1703-1709	JJ	denotes	Washed
T4769	1702-1776	sentence	denotes	Washed filters were exposed to X-ray films for overnight or longer [3,4].
T4768	1701-1702	.	denotes	.
T4767	1697-1698	HYPH	denotes	-
T4766	1698-1701	NN	denotes	min
T4765	1695-1697	CD	denotes	15
T4764	1691-1694	IN	denotes	for
T4763	1688-1690	NN	denotes	°C
T4762	1686-1688	CD	denotes	65
T4761	1683-1685	IN	denotes	at
T4760	1681-1682	-RRB-	denotes	)
T4759	1676-1677	NN	denotes	%
T4758	1673-1676	CD	denotes	0.1
T4757	1671-1673	,	denotes	,
T4756	1667-1668	SYM	denotes	×
T4755	1668-1671	NN	denotes	SSC
T4754	1663-1667	CD	denotes	0.25
T4753	1678-1681	NN	denotes	SDS
T4752	1662-1663	-LRB-	denotes	(
T4751	1659-1661	CD	denotes	II
T4750	1652-1658	NN	denotes	buffer
T4749	1644-1651	NN	denotes	washing
T4748	1639-1643	IN	denotes	with
T4747	1633-1638	RB	denotes	twice
T4746	1626-1632	VBD	denotes	washed
T4745	1621-1625	RB	denotes	then
T4744	1617-1620	CC	denotes	and
T4743	1615-1617	,	denotes	,
T4742	1611-1612	HYPH	denotes	-
T4741	1612-1615	NN	denotes	min
T4740	1609-1611	CD	denotes	15
T4739	1605-1608	IN	denotes	for
T4738	1602-1604	NN	denotes	°C
T4737	1600-1602	CD	denotes	42
T4736	1597-1599	IN	denotes	at
T4735	1595-1596	-RRB-	denotes	)
T4734	1590-1591	NN	denotes	%
T4733	1587-1590	CD	denotes	0.1
T4732	1585-1587	,	denotes	,
T4731	1581-1582	SYM	denotes	×
T4730	1582-1585	NN	denotes	SSC
T4729	1580-1581	CD	denotes	2
T4728	1592-1595	NN	denotes	SDS
T4727	1579-1580	-LRB-	denotes	(
T4726	1577-1578	CD	denotes	I
T4725	1570-1576	NN	denotes	buffer
T4724	1562-1569	VBG	denotes	washing
T4723	1557-1561	IN	denotes	with
T4722	1551-1556	RB	denotes	twice
T4721	1539-1543	VBD	denotes	were
T4720	1544-1550	VBN	denotes	washed
T4719	1531-1538	NNS	denotes	filters
T4718	1527-1530	DT	denotes	The
T4717	1526-1702	sentence	denotes	The filters were washed twice with washing buffer I (2×SSC, 0.1% SDS) at 42°C for 15-min, and then washed twice with washing buffer II (0.25×SSC, 0.1% SDS) at 65°C for 15-min.
T4716	1525-1526	.	denotes	.
T4715	1516-1525	RB	denotes	overnight
T4714	1512-1515	RP	denotes	out
T4713	1499-1503	VBD	denotes	were
T4712	1504-1511	VBN	denotes	carried
T4711	1484-1498	NNS	denotes	Hybridizations
T4710	1483-1526	sentence	denotes	Hybridizations were carried out overnight.
T4709	1482-1483	.	denotes	.
T4708	1481-1482	-RRB-	denotes	)
T4707	1464-1468	NNP	denotes	Life
T4706	1469-1481	NNP	denotes	Technologies
T4705	1463-1464	-LRB-	denotes	(
T4704	1446-1455	NN	denotes	extension
T4703	1439-1445	NN	denotes	primer
T4702	1432-1438	JJ	denotes	random
T4701	1456-1462	NN	denotes	system
T4700	1428-1431	DT	denotes	the
T4699	1422-1427	VBG	denotes	using
T4698	1417-1421	NN	denotes	dCTP
T4697	1412-1413	HYPH	denotes	-
T4696	1413-1416	NN	denotes	32P
T4695	1411-1412	NN	denotes	α
T4694	1406-1410	IN	denotes	with
T4693	1398-1405	VBN	denotes	labeled
T4692	1378-1390	JJ	denotes	untranslated
T4691	1376-1377	SYM	denotes	'
T4690	1391-1397	NN	denotes	region
T4689	1375-1376	CD	denotes	3
T4688	1371-1374	DT	denotes	the
T4687	1367-1370	CC	denotes	and
T4686	1362-1366	NN	denotes	exon
T5626	5084-5092	JJ	denotes	confocal
T5625	5093-5103	NN	denotes	microscope
T5624	5082-5083	DT	denotes	a
T5623	5076-5081	IN	denotes	under
T5622	5064-5068	VBD	denotes	were
T5621	5069-5075	VBN	denotes	viewed
T5620	5058-5063	NNS	denotes	cells
T5619	5054-5057	DT	denotes	The
T5618	5053-5117	sentence	denotes	The cells were viewed under a confocal microscope (Carl Zeiss).
T5617	5052-5053	.	denotes	.
T5616	5029-5045	JJ	denotes	anti-photobleach
T5615	5022-5023	HYPH	denotes	-
T5614	5023-5028	VBN	denotes	based
T5613	5014-5022	NN	denotes	glycerol
T5612	5046-5052	NN	denotes	medium
T5611	5012-5013	DT	denotes	a
T5610	5007-5011	IN	denotes	with
T5609	4998-4999	HYPH	denotes	-
T5608	4993-4998	NN	denotes	cover
T5607	4988-4992	VBD	denotes	were
T5606	4977-4987	NNS	denotes	coverslips
T5605	4973-4976	DT	denotes	the
T5604	4970-4972	IN	denotes	on
T5603	4955-4963	JJ	denotes	neuronal
T5602	4964-4969	NNS	denotes	cells
T5601	4951-4954	DT	denotes	the
T5600	4949-4951	,	denotes	,
T5599	4937-4940	NN	denotes	PBS
T5598	4941-4949	NN	denotes	solution
T5597	4926-4930	NN	denotes	goat
T5596	4931-4936	NN	denotes	serum
T5595	4924-4925	NN	denotes	%
T5594	4923-4924	CD	denotes	1
T5593	4918-4922	IN	denotes	with
T5592	4911-4917	NNS	denotes	washes
T5591	4905-4910	JJ	denotes	final
T5590	4999-5006	VBN	denotes	slipped
T5589	4895-4904	IN	denotes	Following
T5588	4894-5053	sentence	denotes	Following final washes with 1% goat serum PBS solution, the neuronal cells on the coverslips were cover-slipped with a glycerol-based anti-photobleach medium.
T5587	4893-4894	.	denotes	.
T5586	4882-4893	NN	denotes	temperature
T5585	4877-4881	NN	denotes	room
T5584	4874-4876	IN	denotes	at
T5583	4870-4873	NNS	denotes	hrs
T5582	4868-4869	CD	denotes	3
T5581	4864-4867	IN	denotes	for
T5580	4862-4863	-RRB-	denotes	)
T5579	4846-4855	NNP	denotes	Molecular
T5578	4844-4846	,	denotes	,
T5577	4832-4835	NN	denotes	PBS
T5576	4836-4844	NN	denotes	solution
T5575	4821-4825	NN	denotes	goat
T5574	4826-4831	NN	denotes	serum
T5573	4819-4820	NN	denotes	%
T5572	4818-4819	CD	denotes	1
T5571	4815-4817	IN	denotes	in
T5570	4811-4814	CD	denotes	100
T5569	4810-4811	SYM	denotes	:
T5568	4809-4810	CD	denotes	1
T5567	4856-4862	NNP	denotes	Probes
T5566	4808-4809	-LRB-	denotes	(
T5565	4789-4798	JJ	denotes	secondary
T5564	4778-4788	JJ	denotes	conjugated
T5563	4774-4777	CD	denotes	488
T5562	4769-4773	NNP	denotes	Alex
T5561	4799-4807	NN	denotes	antibody
T5125	3265-3266	.	denotes	.
T5124	3264-3265	-RRB-	denotes	]
T5123	3262-3264	CD	denotes	14
T5122	3261-3262	-LRB-	denotes	[
T5121	3249-3250	-RRB-	denotes	)
T5120	3241-3249	NNS	denotes	averages
T5119	3230-3240	NN	denotes	Arithmetic
T5118	3224-3229	VBG	denotes	using
T5117	3211-3216	NN	denotes	Group
T5116	3206-3210	NN	denotes	Pair
T5115	3217-3223	NN	denotes	Method
T5114	3195-3205	JJ	denotes	Unweighted
T5113	3194-3195	-LRB-	denotes	(
T5112	3251-3260	NN	denotes	algorithm
T5111	3188-3193	NN	denotes	UPGMA
T5110	3182-3187	VBG	denotes	using
T5109	3180-3181	-RRB-	denotes	)
T5108	3176-3180	CD	denotes	1.81
T5107	3168-3175	NN	denotes	version
T5106	3167-3168	-LRB-	denotes	(
T5105	3159-3166	NN	denotes	program
T5104	3150-3158	NN	denotes	Clustalw
T5103	3147-3149	IN	denotes	by
T5102	3131-3134	VBD	denotes	was
T5101	3135-3146	VBN	denotes	constructed
T4685	1357-1361	JJ	denotes	last
T4684	1352-1356	IN	denotes	from
T4683	1350-1351	-RRB-	denotes	)
T4682	1344-1347	CD	denotes	350
T4681	1337-1343	IN	denotes	around
T4680	1348-1350	NN	denotes	bp
T4679	1336-1337	-LRB-	denotes	(
T4678	1323-1326	NN	denotes	PCR
T4677	1327-1335	NN	denotes	fragment
T4676	1321-1322	DT	denotes	a
T4675	1316-1320	IN	denotes	with
T4674	1306-1310	NN	denotes	Acdp
T4673	1311-1315	NN	denotes	gene
T4672	1301-1305	DT	denotes	each
T4671	1297-1300	IN	denotes	for
T4670	1285-1289	VBD	denotes	were
T4669	1290-1296	VBN	denotes	probed
T4668	1277-1284	NNS	denotes	filters
T4667	1273-1276	DT	denotes	The
T4666	1272-1483	sentence	denotes	The filters were probed for each Acdp gene with a PCR fragment (around 350 bp) from last exon and the 3' untranslated region labeled with α-32P dCTP using the random primer extension system (Life Technologies).
T4665	1271-1272	.	denotes	.
T4664	1264-1271	NNP	denotes	Origene
T4663	1259-1263	IN	denotes	from
T4662	1244-1248	VBD	denotes	were
T4661	1230-1235	NN	denotes	mouse
T4660	1220-1229	JJ	denotes	different
T4659	1236-1243	NNS	denotes	tissues
T4658	1217-1219	CD	denotes	12
T4657	1206-1216	VBG	denotes	containing
T4656	1249-1258	VBN	denotes	purchased
T4655	1193-1197	NN	denotes	Blot
T4654	1184-1192	NNP	denotes	Northern
T4653	1177-1183	NN	denotes	Choice
T4652	1198-1205	NNS	denotes	filters
T4651	1168-1176	JJ	denotes	Multiple
T4650	1167-1272	sentence	denotes	Multiple Choice Northern Blot filters containing 12 different mouse tissues were purchased from Origene.
T4649	1159-1167	NNS	denotes	analyses
T4648	1154-1158	NN	denotes	blot
T4647	1145-1153	NNP	denotes	Northern
T4607	1142-1143	.	denotes	.
T4606	1128-1131	NN	denotes	DNA
T4605	1132-1142	NN	denotes	sequencing
T4604	1124-1127	NN	denotes	BAC
T4603	1121-1123	IN	denotes	by
T4602	1107-1111	VBD	denotes	were
T4601	1091-1092	SYM	denotes	'
T4600	1090-1091	CD	denotes	5
T4599	1093-1096	NN	denotes	UTR
T4587	1021-1027	VBG	denotes	coding
T4586	1028-1037	NNS	denotes	sequences
T4585	1017-1020	DT	denotes	the
T4584	1014-1016	IN	denotes	of
T4583	1009-1013	JJS	denotes	most
T4582	1043-1053	VBN	denotes	identified
T4581	1004-1008	IN	denotes	Once
T4580	1003-1143	sentence	denotes	Once most of the coding sequences were identified, partial sequence of exon 1 and the 5' UTR sequences were obtained by BAC DNA sequencing.
T4579	1002-1003	.	denotes	.
T4578	994-1002	NN	denotes	sequence
T4577	988-993	JJ	denotes	known
T4576	983-987	IN	denotes	from
T4575	968-975	JJ	denotes	reverse
T4574	976-982	NN	denotes	primer
T4573	966-967	DT	denotes	a
T4572	962-965	CC	denotes	and
T4571	953-961	NN	denotes	sequence
T4570	942-952	VBN	denotes	identified
T4569	936-941	RB	denotes	newly
T4568	931-935	IN	denotes	from
T4567	916-923	JJ	denotes	forward
T4566	924-930	NN	denotes	primer
T4565	914-915	DT	denotes	a
T4564	908-913	VBG	denotes	using
T4563	890-897	RB	denotes	further
T4562	886-889	VBD	denotes	was
T4561	898-907	VBN	denotes	confirmed
T4560	877-885	NN	denotes	sequence
T4559	873-876	DT	denotes	The
T4558	872-1003	sentence	denotes	The sequence was further confirmed using a forward primer from newly identified sequence and a reverse primer from known sequence.
T4557	871-872	.	denotes	.
T4556	852-861	JJ	denotes	automatic
T4555	845-851	NN	denotes	ABI377
T4554	862-871	NN	denotes	sequencer
T4553	842-844	DT	denotes	an
T4552	839-841	IN	denotes	by
T4551	829-838	VBN	denotes	sequenced
T4550	825-828	CC	denotes	and
T4549	821-824	NN	denotes	gel
T4548	813-820	NN	denotes	agarose
T4547	808-812	IN	denotes	from
T4546	791-799	RB	denotes	directly
T4545	786-790	VBD	denotes	were
T4544	800-807	VBN	denotes	excised
T4543	773-776	NN	denotes	PCR
T4542	764-772	JJ	denotes	expected
T4541	777-785	NNS	denotes	products
T4540	760-763	DT	denotes	The
T4539	759-872	sentence	denotes	The expected PCR products were directly excised from agarose gel and sequenced by an ABI377 automatic sequencer.
T4538	758-759	.	denotes	.
T4537	757-758	-RRB-	denotes	)
T4536	755-757	NN	denotes	°C
T4535	753-755	CD	denotes	62
T4534	752-753	-LRB-	denotes	(
T4533	730-739	VBG	denotes	annealing
T4532	740-751	NN	denotes	temperature
T4531	725-729	JJ	denotes	high
T4530	722-724	IN	denotes	at
T4529	709-712	NN	denotes	PCR
T4528	703-708	NN	denotes	round
T4527	697-702	JJ	denotes	first
T4526	713-721	NNS	denotes	products
T4525	693-696	DT	denotes	the
T4524	688-692	IN	denotes	from
T4523	677-682	NN	denotes	mouse
T4522	668-676	JJ	denotes	specific
T4521	683-687	NN	denotes	gene
T4520	664-667	DT	denotes	the
T4519	656-663	VB	denotes	amplify
T4518	653-655	TO	denotes	to
T4517	649-652	RP	denotes	out
T4516	636-640	RB	denotes	then
T4515	632-635	VBD	denotes	was
T4514	617-624	JJ	denotes	forward
T4513	611-616	JJ	denotes	human
T4512	606-610	JJ	denotes	same
T4511	625-631	NN	denotes	primer
T4510	602-605	DT	denotes	the
T4509	598-601	CC	denotes	and
T4508	585-588	NN	denotes	EST
T4507	579-584	NN	denotes	mouse
T4506	589-597	NN	denotes	sequence
T4505	575-578	DT	denotes	the
T4504	570-574	IN	denotes	from
T4503	555-562	JJ	denotes	reverse
T4502	548-554	JJ	denotes	inside
T4501	563-569	NN	denotes	primer
T4500	545-547	DT	denotes	an
T4499	539-544	VBG	denotes	using
T4498	641-648	VBN	denotes	carried
T4497	528-534	JJ	denotes	nested
T4496	535-538	NN	denotes	PCR
T4495	526-527	DT	denotes	A
T4494	525-759	sentence	denotes	A nested PCR using an inside reverse primer from the mouse EST sequence and the same human forward primer was then carried out to amplify the specific mouse gene from the first round PCR products at high annealing temperature (62°C).
T4493	524-525	.	denotes	.
T4492	523-524	-RRB-	denotes	)
T4491	521-523	NN	denotes	°C
T4490	518-519	SYM	denotes	–
T4489	519-521	CD	denotes	50
T4488	516-518	CD	denotes	45
T4487	515-516	-LRB-	denotes	(
T4486	493-502	VBG	denotes	annealing
T4485	503-514	NN	denotes	temperature
T4484	489-492	JJ	denotes	low
T4483	484-488	RB	denotes	very
T4482	481-483	IN	denotes	at
T4481	476-480	NN	denotes	cDNA
T4480	470-475	NN	denotes	mouse
T4479	465-469	IN	denotes	from
T4478	446-455	NN	denotes	homologue
T4477	456-464	NN	denotes	sequence
T4476	442-445	DT	denotes	the
T4475	434-441	VB	denotes	amplify
T4474	431-433	TO	denotes	to
T4473	421-425	VBD	denotes	were
T4472	410-413	NN	denotes	EST
T4471	404-409	NN	denotes	mouse
T4470	414-420	NN	denotes	marker
T4469	400-403	DT	denotes	the
T4468	395-399	IN	denotes	from
T4467	380-387	JJ	denotes	reverse
T4466	374-379	NN	denotes	mouse
T4465	388-394	NN	denotes	primer
T4464	372-373	DT	denotes	a
T4463	368-371	CC	denotes	and
T4462	366-367	-RRB-	denotes	)
T4461	361-366	NN	denotes	codon
T4460	355-360	NN	denotes	start
T4459	349-354	IN	denotes	after
T4884	2092-2098	NN	denotes	PIERCE
T4883	2091-2092	-LRB-	denotes	(
T4882	2087-2090	NN	denotes	ECL
T4881	2081-2086	VBG	denotes	Using
T4880	2077-2080	RP	denotes	out
T4879	2064-2068	VBD	denotes	were
T4878	2069-2076	VBN	denotes	carried
T4877	2058-2063	NNS	denotes	blots
T4876	2050-2057	NNP	denotes	Western
T4875	2049-2128	sentence	denotes	Western blots were carried out Using ECL (PIERCE) as described previously [1].
T4874	2048-2049	.	denotes	.
T4873	2047-2048	-RRB-	denotes	]
T4872	2046-2047	CD	denotes	5
T4871	2045-2046	-LRB-	denotes	[
T4870	2032-2044	RB	denotes	respectively
T4869	2030-2032	,	denotes	,
T4868	2022-2030	VBN	denotes	reported
T4867	2019-2021	IN	denotes	as
T4866	2006-2010	NN	denotes	Acdp
T4865	2011-2018	NNS	denotes	members
T4864	2002-2005	DT	denotes	all
T4863	1998-2001	CC	denotes	and
T4862	1992-1997	NN	denotes	Acdp1
T4861	1988-1991	IN	denotes	for
T4860	1975-1987	RB	denotes	specifically
T4859	1964-1974	NNS	denotes	antibodies
T4858	1961-1963	IN	denotes	of
T4857	1950-1960	NN	denotes	generation
T4856	1946-1949	IN	denotes	for
T4855	1936-1940	VBD	denotes	were
T4854	1934-1935	-RRB-	denotes	)
T4853	1931-1934	NN	denotes	ACD
T4852	1930-1931	-LRB-	denotes	(
T4851	1913-1922	VBN	denotes	conserved
T4850	1923-1929	NN	denotes	domain
T4849	1909-1912	DT	denotes	the
T4848	1905-1908	CC	denotes	and
T4847	1894-1895	HYPH	denotes	-
T4846	1893-1894	NN	denotes	C
T4845	1889-1892	CC	denotes	and
T4844	1887-1888	HYPH	denotes	-
T4843	1886-1887	NN	denotes	N
T4842	1895-1904	NNS	denotes	terminals
T4841	1880-1885	NN	denotes	Acdp1
T4840	1875-1879	IN	denotes	from
T4839	1873-1874	-RRB-	denotes	)
T4838	1856-1862	NN	denotes	limpet
T4837	1863-1873	NN	denotes	hemacyanin
T4836	1848-1855	NN	denotes	keyhole
T4835	1847-1848	-LRB-	denotes	(
T4834	1843-1846	NN	denotes	KLH
T4833	1840-1842	IN	denotes	to
T4832	1833-1839	VBN	denotes	linked
T4831	1941-1945	VBN	denotes	used
T4830	1824-1832	NNS	denotes	Peptides
T4829	1823-2049	sentence	denotes	Peptides linked to KLH (keyhole limpet hemacyanin) from Acdp1 N- and C-terminals and the conserved domain (ACD) were used for generation of antibodies specifically for Acdp1 and all Acdp members as reported, respectively [5].
T4828	1815-1823	NNS	denotes	analyses
T4827	1810-1814	NN	denotes	blot
T4826	1802-1809	NNP	denotes	Western
T4825	1798-1801	CC	denotes	and
T4824	1787-1797	NN	denotes	production
T4823	1778-1786	NN	denotes	Antibody
T4458	348-349	-LRB-	denotes	(
T4457	334-340	NN	denotes	coding
T4456	329-333	NN	denotes	cDNA
T4455	327-328	SYM	denotes	'
T4454	326-327	CD	denotes	5
T4453	321-325	NN	denotes	ACDP
T4452	315-320	JJ	denotes	human
T4451	341-347	NN	denotes	region
T4450	311-314	DT	denotes	the
T4449	304-310	IN	denotes	within
T4448	426-430	VBN	denotes	used
T4447	289-296	JJ	denotes	forward
T4446	297-303	NN	denotes	primer
T4445	287-288	DT	denotes	A
T4444	286-525	sentence	denotes	A forward primer within the human ACDP 5' cDNA coding region (after start codon) and a mouse reverse primer from the mouse EST marker were used to amplify the homologue sequence from mouse cDNA at very low annealing temperature (45–50°C).
T4443	285-286	.	denotes	.
T4442	274-278	NN	denotes	Acdp
T4441	279-285	NN	denotes	member
T4440	269-273	DT	denotes	each
T4439	266-268	IN	denotes	to
T4438	252-265	VBG	denotes	corresponding
T4437	244-251	NNS	denotes	markers
T4436	240-243	NN	denotes	EST
T4435	236-239	IN	denotes	for
T4434	223-226	NN	denotes	EST
T4433	217-222	NN	denotes	mouse
T4432	227-235	NN	denotes	database
T4431	213-216	DT	denotes	the
T4430	206-212	VB	denotes	search
T4429	203-205	TO	denotes	to
T4428	193-197	VBD	denotes	were
T4427	180-182	NN	denotes	AA
T4426	183-192	NNS	denotes	sequences
T4425	170-179	VBN	denotes	predicted
T4424	166-169	CC	denotes	and
T4423	155-159	NN	denotes	ACDP
T4422	149-154	JJ	denotes	human
T4421	160-165	NNS	denotes	cDNAs
T4420	145-148	DT	denotes	the
T4419	143-145	,	denotes	,
T4418	198-202	VBN	denotes	used
T4417	136-143	RB	denotes	Briefly
T4416	135-286	sentence	denotes	Briefly, the human ACDP cDNAs and predicted AA sequences were used to search the mouse EST database for EST markers corresponding to each Acdp member.
T4415	134-135	.	denotes	.
T4414	133-134	-RRB-	denotes	]
T4413	132-133	CD	denotes	2
T4412	131-132	-LRB-	denotes	[
T4411	111-121	RB	denotes	previously
T4410	122-130	VBN	denotes	reported
T4409	108-110	IN	denotes	as
T4408	88-97	NN	denotes	homologue
T4407	98-107	NNS	denotes	sequences
T4406	82-87	JJ	denotes	human
T4405	79-81	IN	denotes	on
T4404	73-78	VBN	denotes	based
T4403	59-62	VBD	denotes	was
T4402	47-51	NN	denotes	gene
T4401	42-46	NN	denotes	Acdp
T4400	52-58	NN	denotes	family
T4399	38-41	DT	denotes	the
T4398	35-37	IN	denotes	of
T4397	63-72	VBN	denotes	performed
T4396	27-34	NN	denotes	cloning
T4395	22-26	NN	denotes	cDNA
T4394	21-135	sentence	denotes	cDNA cloning of the Acdp gene family was performed based on human homologue sequences as previously reported [2].
T4393	14-21	NN	denotes	cloning
T4392	9-13	NN	denotes	cDNA
T4598	1086-1089	DT	denotes	the
T4597	1082-1085	CC	denotes	and
T4596	1080-1081	CD	denotes	1
T4595	1097-1106	NNS	denotes	sequences
T4594	1075-1079	NN	denotes	exon
T4593	1072-1074	IN	denotes	of
T4592	1063-1071	NN	denotes	sequence
T4591	1055-1062	JJ	denotes	partial
T4590	1053-1055	,	denotes	,
T4589	1112-1120	VBN	denotes	obtained
T4588	1038-1042	VBD	denotes	were
T5719	5443-5444	.	denotes	.
T5679	5255-5261	NN	denotes	number
T5712	5416-5421	NN	denotes	Acdp3
T5711	5415-5416	-LRB-	denotes	(
T5710	5406-5414	NN	denotes	AF216964
T5709	5404-5406	,	denotes	,
T5708	5403-5404	-RRB-	denotes	)
T5707	5398-5403	NN	denotes	Acdp2
T5706	5397-5398	-LRB-	denotes	(
T5705	5388-5396	NN	denotes	AF216961
T5704	5386-5388	,	denotes	,
T5703	5385-5386	-RRB-	denotes	)
T5702	5380-5385	NN	denotes	Acdp1
R2573	T4392	T4393	compound	cDNA,cloning
R2574	T4395	T4396	compound	cDNA,cloning
R2575	T4396	T4397	nsubjpass	cloning,performed
R2576	T4398	T4396	prep	of,cloning
R2577	T4399	T4400	det	the,family
R2578	T4400	T4398	pobj	family,of
R2579	T4401	T4400	compound	Acdp,family
R2580	T4402	T4400	compound	gene,family
R2581	T4403	T4397	auxpass	was,performed
R2582	T4404	T4397	prep	based,performed
R2583	T4405	T4404	prep	on,based
R2584	T4406	T4407	amod	human,sequences
R2585	T4407	T4405	pobj	sequences,on
R2586	T4408	T4407	compound	homologue,sequences
R2587	T4409	T4410	mark	as,reported
R2588	T4410	T4397	advcl	reported,performed
R2589	T4411	T4410	advmod	previously,reported
R2590	T4412	T4413	punct	[,2
R2591	T4413	T4397	parataxis	2,performed
R2592	T4414	T4413	punct	],2
R2593	T4415	T4397	punct	.,performed
R2594	T4417	T4418	advmod	Briefly,used
R2595	T4419	T4418	punct	", ",used
R2596	T4420	T4421	det	the,cDNAs
R2597	T4421	T4418	nsubjpass	cDNAs,used
R2598	T4422	T4421	amod	human,cDNAs
R2599	T4423	T4421	compound	ACDP,cDNAs
R2600	T4424	T4421	cc	and,cDNAs
R2601	T4425	T4426	amod	predicted,sequences
R2602	T4426	T4421	conj	sequences,cDNAs
R2603	T4427	T4426	compound	AA,sequences
R2604	T4428	T4418	auxpass	were,used
R2605	T4429	T4430	aux	to,search
R2606	T4430	T4418	advcl	search,used
R2607	T4431	T4432	det	the,database
R2608	T4432	T4430	dobj	database,search
R2609	T4433	T4432	compound	mouse,database
R2610	T4434	T4432	compound	EST,database
R2611	T4435	T4430	prep	for,search
R2612	T4436	T4437	compound	EST,markers
R2613	T4437	T4435	pobj	markers,for
R2614	T4438	T4437	acl	corresponding,markers
R2615	T4439	T4438	prep	to,corresponding
R2616	T4440	T4441	det	each,member
R2617	T4441	T4439	pobj	member,to
R2618	T4442	T4441	compound	Acdp,member
R2619	T4443	T4418	punct	.,used
R2620	T4445	T4446	det	A,primer
R2621	T4446	T4448	nsubjpass	primer,used
R2622	T4447	T4446	amod	forward,primer
R2623	T4449	T4446	prep	within,primer
R2624	T4450	T4451	det	the,region
R2625	T4451	T4449	pobj	region,within
R2626	T4452	T4451	amod	human,region
R2627	T4453	T4451	nmod	ACDP,region
R2628	T4454	T4451	nummod	5,region
R2629	T4455	T4454	punct	',5
R2630	T4456	T4451	compound	cDNA,region
R2631	T4457	T4451	compound	coding,region
R2632	T4458	T4446	punct	(,primer
R2633	T4459	T4446	prep	after,primer
R2634	T4460	T4461	compound	start,codon
R2635	T4461	T4459	pobj	codon,after
R2636	T4462	T4446	punct	),primer
R2637	T4463	T4446	cc	and,primer
R2638	T4464	T4465	det	a,primer
R2639	T4465	T4446	conj	primer,primer
R2640	T4466	T4465	nmod	mouse,primer
R2641	T4467	T4465	amod	reverse,primer
R2642	T4468	T4465	prep	from,primer
R2643	T4469	T4470	det	the,marker
R2644	T4470	T4468	pobj	marker,from
R2645	T4471	T4470	compound	mouse,marker
R2646	T4472	T4470	compound	EST,marker
R2647	T4473	T4448	auxpass	were,used
R2648	T4474	T4475	aux	to,amplify
R2649	T4475	T4448	advcl	amplify,used
R2650	T4476	T4477	det	the,sequence
R2651	T4477	T4475	dobj	sequence,amplify
R2652	T4478	T4477	compound	homologue,sequence
R2653	T4479	T4475	prep	from,amplify
R2654	T4480	T4481	compound	mouse,cDNA
R2655	T4481	T4479	pobj	cDNA,from
R2656	T4482	T4475	prep	at,amplify
R2657	T4483	T4484	advmod	very,low
R2658	T4484	T4485	amod	low,temperature
R2659	T4485	T4482	pobj	temperature,at
R2660	T4486	T4485	amod	annealing,temperature
R2661	T4487	T4485	punct	(,temperature
R2662	T4488	T4489	quantmod	45,50
R2663	T4489	T4491	nummod	50,°C
R2664	T4490	T4489	punct	–,50
R2665	T4491	T4485	appos	°C,temperature
R2666	T4492	T4448	punct	),used
R2667	T4493	T4448	punct	.,used
R2668	T4495	T4496	det	A,PCR
R2669	T4496	T4498	nsubjpass	PCR,carried
R2670	T4497	T4496	amod	nested,PCR
R2671	T4499	T4496	acl	using,PCR
R2672	T4500	T4501	det	an,primer
R2673	T4501	T4499	dobj	primer,using
R2674	T4502	T4501	amod	inside,primer
R2675	T4503	T4501	amod	reverse,primer
R2676	T4504	T4501	prep	from,primer
R2677	T4505	T4506	det	the,sequence
R2678	T4506	T4504	pobj	sequence,from
R2679	T4507	T4506	compound	mouse,sequence
R2680	T4508	T4506	compound	EST,sequence
R2681	T4509	T4501	cc	and,primer
R2682	T4510	T4511	det	the,primer
R2683	T4511	T4501	conj	primer,primer
R2684	T4512	T4511	amod	same,primer
R2685	T4513	T4511	amod	human,primer
R2686	T4514	T4511	amod	forward,primer
R2687	T4515	T4498	auxpass	was,carried
R2688	T4516	T4498	advmod	then,carried
R2689	T4517	T4498	prt	out,carried
R2690	T4518	T4519	aux	to,amplify
R2691	T4519	T4498	advcl	amplify,carried
R2692	T4520	T4521	det	the,gene
R2693	T4521	T4519	dobj	gene,amplify
R2694	T4522	T4521	amod	specific,gene
R2695	T4523	T4521	compound	mouse,gene
R2696	T4524	T4521	prep	from,gene
R2697	T4525	T4526	det	the,products
R2698	T4526	T4524	pobj	products,from
R2699	T4527	T4528	amod	first,round
R2700	T4528	T4526	compound	round,products
R2701	T4529	T4526	compound	PCR,products
R2702	T4530	T4519	prep	at,amplify
R2703	T4531	T4532	amod	high,temperature
R2704	T4532	T4530	pobj	temperature,at
R2705	T4533	T4532	amod	annealing,temperature
R2706	T4534	T4532	punct	(,temperature
R2707	T4535	T4536	nummod	62,°C
R2708	T4536	T4532	appos	°C,temperature
R2709	T4537	T4498	punct	),carried
R2710	T4538	T4498	punct	.,carried
R2711	T4540	T4541	det	The,products
R2712	T4541	T4544	nsubjpass	products,excised
R2713	T4542	T4541	amod	expected,products
R2714	T4543	T4541	compound	PCR,products
R2715	T4545	T4544	auxpass	were,excised
R2716	T4546	T4544	advmod	directly,excised
R2717	T4547	T4544	prep	from,excised
R2718	T4548	T4549	compound	agarose,gel
R2719	T4549	T4547	pobj	gel,from
R2720	T4550	T4544	cc	and,excised
R2721	T4551	T4544	conj	sequenced,excised
R2722	T4552	T4551	prep	by,sequenced
R2723	T4553	T4554	det	an,sequencer
R2724	T4554	T4552	pobj	sequencer,by
R2725	T4555	T4554	nmod	ABI377,sequencer
R2726	T4556	T4554	amod	automatic,sequencer
R2727	T4557	T4544	punct	.,excised
R2728	T4559	T4560	det	The,sequence
R2729	T4560	T4561	nsubjpass	sequence,confirmed
R2730	T4562	T4561	auxpass	was,confirmed
R2731	T4563	T4561	advmod	further,confirmed
R2732	T4564	T4561	advcl	using,confirmed
R2733	T4565	T4566	det	a,primer
R2734	T4566	T4564	dobj	primer,using
R2735	T4567	T4566	amod	forward,primer
R2736	T4568	T4566	prep	from,primer
R2737	T4569	T4570	advmod	newly,identified
R2738	T4570	T4571	amod	identified,sequence
R2739	T4571	T4568	pobj	sequence,from
R2740	T4572	T4566	cc	and,primer
R2741	T4573	T4574	det	a,primer
R2742	T4574	T4566	conj	primer,primer
R2743	T4575	T4574	amod	reverse,primer
R2744	T4576	T4574	prep	from,primer
R2745	T4577	T4578	amod	known,sequence
R2746	T4578	T4576	pobj	sequence,from
R2747	T4579	T4561	punct	.,confirmed
R2748	T4581	T4582	mark	Once,identified
R2749	T4582	T4589	advcl	identified,obtained
R2750	T4583	T4582	nsubjpass	most,identified
R2751	T4584	T4583	prep	of,most
R2752	T4585	T4586	det	the,sequences
R2753	T4586	T4584	pobj	sequences,of
R2754	T4587	T4586	amod	coding,sequences
R2755	T4588	T4582	auxpass	were,identified
R2756	T4590	T4589	punct	", ",obtained
R2757	T4591	T4592	amod	partial,sequence
R2758	T4592	T4589	nsubjpass	sequence,obtained
R2759	T4593	T4592	prep	of,sequence
R2760	T4594	T4595	nmod	exon,sequences
R2761	T4595	T4593	pobj	sequences,of
R2762	T4596	T4594	nummod	1,exon
R2763	T4597	T4594	cc	and,exon
R2764	T4598	T4599	det	the,UTR
R2765	T4599	T4594	conj	UTR,exon
R2766	T4600	T4599	nummod	5,UTR
R2767	T4601	T4600	punct	',5
R2768	T4602	T4589	auxpass	were,obtained
R2769	T4603	T4589	prep	by,obtained
R2770	T4604	T4605	compound	BAC,sequencing
R2771	T4605	T4603	pobj	sequencing,by
R2772	T4606	T4605	compound	DNA,sequencing
R2773	T4607	T4589	punct	.,obtained
R2774	T4647	T4648	compound	Northern,blot
R2775	T4648	T4649	compound	blot,analyses
R2776	T4651	T4652	amod	Multiple,filters
R2777	T4652	T4656	nsubjpass	filters,purchased
R2778	T4653	T4652	compound	Choice,filters
R2779	T4654	T4655	compound	Northern,Blot
R2780	T4655	T4652	compound	Blot,filters
R2781	T4657	T4652	acl	containing,filters
R2782	T4658	T4659	nummod	12,tissues
R2783	T4659	T4657	dobj	tissues,containing
R2784	T4660	T4659	amod	different,tissues
R2785	T4661	T4659	compound	mouse,tissues
R2786	T4662	T4656	auxpass	were,purchased
R2787	T4663	T4656	prep	from,purchased
R2788	T4664	T4663	pobj	Origene,from
R2789	T4665	T4656	punct	.,purchased
R2790	T4667	T4668	det	The,filters
R2791	T4668	T4669	nsubjpass	filters,probed
R2792	T4670	T4669	auxpass	were,probed
R2793	T4671	T4669	prep	for,probed
R2794	T4672	T4673	det	each,gene
R2795	T4673	T4671	pobj	gene,for
R2796	T4674	T4673	compound	Acdp,gene
R2797	T4675	T4669	prep	with,probed
R2798	T4676	T4677	det	a,fragment
R2799	T4677	T4675	pobj	fragment,with
R2800	T4678	T4677	compound	PCR,fragment
R2801	T4679	T4680	punct	(,bp
R2802	T4680	T4677	parataxis	bp,fragment
R2803	T4681	T4682	quantmod	around,350
R2804	T4682	T4680	nummod	350,bp
R2805	T4683	T4680	punct	),bp
R2806	T4684	T4677	prep	from,fragment
R2807	T4685	T4686	amod	last,exon
R2808	T4686	T4684	pobj	exon,from
R2809	T4687	T4669	cc	and,probed
R2810	T4688	T4689	det	the,3
R2811	T4689	T4690	nummod	3,region
R2812	T4690	T4693	nsubj	region,labeled
R2813	T4691	T4689	punct	',3
R2814	T4692	T4689	amod	untranslated,3
R2815	T4693	T4669	conj	labeled,probed
R2816	T4694	T4693	prep	with,labeled
R2817	T4695	T4696	compound	α,32P
R2818	T4696	T4698	compound	32P,dCTP
R2819	T4697	T4696	punct	-,32P
R2820	T4698	T4694	pobj	dCTP,with
R2821	T4699	T4693	advcl	using,labeled
R2822	T4700	T4701	det	the,system
R2823	T4701	T4699	dobj	system,using
R2824	T4702	T4701	amod	random,system
R2825	T4703	T4701	compound	primer,system
R2826	T4704	T4701	compound	extension,system
R2827	T4705	T4706	punct	(,Technologies
R2828	T4706	T4701	parataxis	Technologies,system
R2829	T4707	T4706	compound	Life,Technologies
R2830	T4708	T4706	punct	),Technologies
R2831	T4709	T4693	punct	.,labeled
R2832	T4711	T4712	nsubjpass	Hybridizations,carried
R2833	T4713	T4712	auxpass	were,carried
R2834	T4714	T4712	prt	out,carried
R2835	T4715	T4712	advmod	overnight,carried
R2836	T4716	T4712	punct	.,carried
R2837	T4718	T4719	det	The,filters
R2838	T4719	T4720	nsubjpass	filters,washed
R2839	T4721	T4720	auxpass	were,washed
R2840	T4722	T4720	advmod	twice,washed
R2841	T4723	T4720	prep	with,washed
R2842	T4724	T4725	amod	washing,buffer
R2843	T4725	T4723	pobj	buffer,with
R2844	T4726	T4725	nummod	I,buffer
R2845	T4727	T4728	punct	(,SDS
R2846	T4728	T4725	parataxis	SDS,buffer
R2847	T4729	T4730	nummod	2,SSC
R2848	T4730	T4728	dep	SSC,SDS
R2849	T4731	T4730	punct	×,SSC
R2850	T4732	T4728	punct	", ",SDS
R2851	T4733	T4734	nummod	0.1,%
R2852	T4734	T4728	compound	%,SDS
R2853	T4735	T4728	punct	),SDS
R2854	T4736	T4720	prep	at,washed
R2855	T4737	T4738	nummod	42,°C
R2856	T4738	T4736	pobj	°C,at
R2857	T4739	T4720	prep	for,washed
R2858	T4740	T4741	nummod	15,min
R2859	T4741	T4739	pobj	min,for
R2860	T4742	T4741	punct	-,min
R2861	T4743	T4720	punct	", ",washed
R2862	T4744	T4720	cc	and,washed
R2863	T4745	T4746	advmod	then,washed
R2864	T4746	T4720	conj	washed,washed
R2865	T4747	T4746	advmod	twice,washed
R2866	T4748	T4746	prep	with,washed
R2867	T4749	T4750	compound	washing,buffer
R2868	T4750	T4748	pobj	buffer,with
R2869	T4751	T4750	nummod	II,buffer
R2870	T4752	T4753	punct	(,SDS
R2871	T4753	T4750	parataxis	SDS,buffer
R2872	T4754	T4755	nummod	0.25,SSC
R2873	T4755	T4753	dep	SSC,SDS
R2874	T4756	T4755	punct	×,SSC
R2875	T4757	T4753	punct	", ",SDS
R2876	T4758	T4759	nummod	0.1,%
R2877	T4759	T4753	compound	%,SDS
R2878	T4760	T4753	punct	),SDS
R2879	T4761	T4746	prep	at,washed
R2880	T4762	T4763	nummod	65,°C
R2881	T4763	T4761	pobj	°C,at
R2882	T4764	T4746	prep	for,washed
R2883	T4765	T4766	nummod	15,min
R2884	T4766	T4764	pobj	min,for
R2885	T4767	T4766	punct	-,min
R2886	T4768	T4720	punct	.,washed
R2887	T4770	T4771	amod	Washed,filters
R2888	T4771	T4772	nsubjpass	filters,exposed
R2889	T4773	T4772	auxpass	were,exposed
R2890	T4774	T4772	prep	to,exposed
R2891	T4775	T4776	compound	X-ray,films
R2892	T4776	T4774	pobj	films,to
R2893	T4777	T4772	prep	for,exposed
R2894	T4778	T4777	pcomp	overnight,for
R2895	T4779	T4778	cc	or,overnight
R2896	T4780	T4778	conj	longer,overnight
R2897	T4781	T4782	punct	[,4
R2898	T4782	T4772	parataxis	4,exposed
R2899	T4783	T4782	nummod	3,4
R2900	T4784	T4782	punct	",",4
R2901	T4785	T4782	punct	],4
R2902	T4786	T4772	punct	.,exposed
R2904	T4823	T4824	compound	Antibody,production
R2905	T4825	T4824	cc	and,production
R2906	T4826	T4827	compound	Western,blot
R2907	T4827	T4828	compound	blot,analyses
R2908	T4828	T4824	conj	analyses,production
R2909	T4830	T4831	nsubjpass	Peptides,used
R2910	T4832	T4830	acl	linked,Peptides
R2911	T4833	T4832	prep	to,linked
R2912	T4834	T4833	pobj	KLH,to
R2913	T4835	T4834	punct	(,KLH
R2914	T4836	T4837	compound	keyhole,hemacyanin
R2915	T4837	T4834	appos	hemacyanin,KLH
R2916	T4838	T4837	compound	limpet,hemacyanin
R2917	T4839	T4833	punct	),to
R2918	T4840	T4833	prep	from,to
R2919	T4841	T4842	nmod	Acdp1,terminals
R2920	T4842	T4840	pobj	terminals,from
R2921	T4843	T4842	nmod	N,terminals
R2922	T4844	T4843	punct	-,N
R2923	T4845	T4843	cc	and,N
R2924	T4846	T4843	conj	C,N
R2925	T4847	T4842	punct	-,terminals
R2926	T4848	T4842	cc	and,terminals
R2927	T4849	T4850	det	the,domain
R2928	T4850	T4842	conj	domain,terminals
R2929	T4851	T4850	amod	conserved,domain
R2930	T4852	T4850	punct	(,domain
R2931	T4853	T4850	appos	ACD,domain
R2932	T4854	T4831	punct	),used
R2933	T4855	T4831	auxpass	were,used
R2934	T4856	T4831	prep	for,used
R2935	T4857	T4856	pobj	generation,for
R2936	T4858	T4857	prep	of,generation
R2937	T4859	T4858	pobj	antibodies,of
R2938	T4860	T4861	advmod	specifically,for
R2939	T4861	T4856	prep	for,for
R2940	T4862	T4861	pobj	Acdp1,for
R2941	T4863	T4862	cc	and,Acdp1
R2942	T4864	T4865	det	all,members
R2943	T4865	T4862	conj	members,Acdp1
R2944	T4866	T4865	compound	Acdp,members
R2945	T4867	T4868	mark	as,reported
R2946	T4868	T4831	advcl	reported,used
R2947	T4869	T4831	punct	", ",used
R2948	T4870	T4831	advmod	respectively,used
R2949	T4871	T4872	punct	[,5
R2950	T4872	T4831	parataxis	5,used
R2951	T4873	T4872	punct	],5
R2952	T4874	T4831	punct	.,used
R2953	T4876	T4877	compound	Western,blots
R2954	T4877	T4878	nsubjpass	blots,carried
R2955	T4879	T4878	auxpass	were,carried
R2956	T4880	T4878	prt	out,carried
R2957	T4881	T4878	advcl	Using,carried
R2958	T4882	T4881	dobj	ECL,Using
R2959	T4883	T4884	punct	(,PIERCE
R2960	T4884	T4882	parataxis	PIERCE,ECL
R2961	T4885	T4884	punct	),PIERCE
R2962	T4886	T4887	mark	as,described
R2963	T4887	T4878	advcl	described,carried
R2964	T4888	T4887	advmod	previously,described
R2965	T4889	T4890	punct	[,1
R2966	T4890	T4878	parataxis	1,carried
R2967	T4891	T4890	punct	],1
R2968	T4892	T4878	punct	.,carried
R2969	T4894	T4895	det	The,membranes
R2970	T4895	T4896	nsubjpass	membranes,washed
R2971	T4897	T4896	auxpass	were,washed
R2972	T4898	T4896	advmod	extensively,washed
R2973	T4899	T4896	prep	after,washed
R2974	T4900	T4899	pobj	incubation,after
R2975	T4901	T4900	prep	with,incubation
R2976	T4902	T4903	amod	primary,antibodies
R2977	T4903	T4901	pobj	antibodies,with
R2978	T4904	T4902	cc	and,primary
R2979	T4905	T4902	conj	secondary,primary
R2980	T4906	T4896	cc	and,washed
R2981	T4907	T4908	auxpass	were,developed
R2982	T4908	T4896	conj	developed,washed
R2983	T4909	T4908	advmod	then,developed
R2984	T4910	T4908	prep	with,developed
R2985	T4911	T4912	compound	X-ray,films
R2986	T4912	T4910	pobj	films,with
R2987	T4913	T4912	prep	with,films
R2988	T4914	T4915	amod	optimal,time
R2989	T4915	T4913	pobj	time,with
R2990	T4916	T4915	compound	exposure,time
R2991	T4917	T4896	punct	.,washed
R2992	T4941	T4942	compound	Chromosome,localization
R2993	T4944	T4945	det	The,panel
R2994	T4945	T4950	nsubjpass	panel,used
R2995	T4946	T4945	compound	T31,panel
R2996	T4947	T4945	compound	mouse,panel
R2997	T4948	T4945	compound	radiation,panel
R2998	T4949	T4945	compound	hybrid,panel
R2999	T4951	T4945	prep	from,panel
R3000	T4952	T4953	compound	Research,Genetics
R3001	T4953	T4951	pobj	Genetics,from
R3002	T4954	T4950	auxpass	was,used
R3003	T4955	T4956	aux	to,map
R3004	T4956	T4950	advcl	map,used
R3005	T4957	T4958	det	the,location
R3006	T4958	T4956	dobj	location,map
R3007	T4959	T4958	compound	chromosome,location
R3008	T4960	T4958	prep	of,location
R3009	T4961	T4962	det	each,member
R3010	T4962	T4960	pobj	member,of
R3011	T4963	T4962	compound	Acdp,member
R3012	T4964	T4950	punct	.,used
R3013	T4966	T4967	nsubjpass	Primers,used
R3014	T4968	T4966	prep	from,Primers
R3015	T4969	T4970	nummod	3,UTR
R3016	T4970	T4968	pobj	UTR,from
R3017	T4971	T4969	punct	',3
R3018	T4972	T4970	prep	of,UTR
R3019	T4973	T4974	det	each,member
R3020	T4974	T4972	pobj	member,of
R3021	T4975	T4974	compound	Acdp,member
R3022	T4976	T4967	auxpass	were,used
R3023	T4977	T4978	aux	to,amplify
R3024	T4978	T4967	advcl	amplify,used
R3025	T4979	T4980	det	the,clones
R3026	T4980	T4978	dobj	clones,amplify
R3027	T4981	T4980	nummod	100,clones
R3028	T4982	T4980	nmod	radiation,clones
R3029	T4983	T4980	amod	hybrid,clones
R3030	T4984	T4980	acl	representing,clones
R3031	T4985	T4986	det	the,genome
R3032	T4986	T4984	dobj	genome,representing
R3033	T4987	T4986	compound	mouse,genome
R3034	T4988	T4967	punct	.,used
R3035	T4990	T4991	det	The,data
R3036	T4991	T4992	nsubjpass	data,submitted
R3037	T4993	T4992	auxpass	were,submitted
R3038	T4994	T4992	prep	to,submitted
R3039	T4995	T4996	det	the,Database
R3040	T4996	T4994	pobj	Database,to
R3041	T4997	T4998	compound	Jackson,Laboratory
R3042	T4998	T4996	compound	Laboratory,Database
R3043	T4999	T5000	compound	Mouse,Hybrid
R3044	T5000	T4996	compound	Hybrid,Database
R3045	T5001	T5000	compound	Radiation,Hybrid
R3046	T5002	T4992	prep	for,submitted
R3047	T5003	T5002	pobj	analysis,for
R3048	T5004	T4992	punct	.,submitted
R3049	T5023	T5024	compound	Sequence,analyses
R3050	T5026	T5027	compound	Sequence,assembly
R3051	T5027	T5028	nsubjpass	assembly,performed
R3052	T5029	T5028	auxpass	was,performed
R3053	T5030	T5028	prep	with,performed
R3054	T5031	T5032	compound	program,Sequencher
R3055	T5032	T5030	pobj	Sequencher,with
R3056	T5033	T5034	punct	(,Corp
R3057	T5034	T5032	parataxis	Corp,Sequencher
R3058	T5035	T5034	compound	Gene,Corp
R3059	T5036	T5034	compound	Codes,Corp
R3060	T5037	T5034	punct	),Corp
R3061	T5038	T5028	punct	.,performed
R3062	T5040	T5041	nmod	Protein,homology
R3063	T5041	T5044	compound	homology,searches
R3064	T5042	T5040	cc	and,Protein
R3065	T5043	T5040	conj	DNA,Protein
R3066	T5044	T5045	nsubjpass	searches,carried
R3067	T5046	T5045	auxpass	were,carried
R3068	T5047	T5045	prt	out,carried
R3069	T5048	T5045	prep	with,carried
R3070	T5049	T5050	nmod	tblastn,programs
R3071	T5050	T5048	pobj	programs,with
R3072	T5051	T5049	punct	", ",tblastn
R3073	T5052	T5049	conj	tblastx,tblastn
R3074	T5053	T5052	punct	", ",tblastx
R3075	T5054	T5052	conj	blastp,tblastx
R3076	T5055	T5054	cc	and,blastp
R3077	T5056	T5054	conj	blastn,blastp
R3078	T5057	T5045	punct	.,carried
R3079	T5059	T5060	amod	Multiple,alignments
R3080	T5060	T5062	nsubjpass	alignments,performed
R3081	T5061	T5060	compound	sequence,alignments
R3082	T5063	T5062	auxpass	were,performed
R3083	T5064	T5062	prep	with,performed
R3084	T5065	T5066	nmod	GeneDoc,alignment
R3085	T5066	T5064	pobj	alignment,with
R3086	T5067	T5065	cc	and,GeneDoc
R3087	T5068	T5065	conj	pairwise,GeneDoc
R3088	T5069	T5066	compound	sequence,alignment
R3089	T5070	T5062	punct	.,performed
R3090	T5072	T5073	amod	Multiple,programs
R3091	T5073	T5074	nsubjpass	programs,used
R3092	T5075	T5073	prep	including,programs
R3093	T5076	T5077	compound	BCM,Launcher
R3094	T5077	T5075	pobj	Launcher,including
R3095	T5078	T5077	compound	Search,Launcher
R3096	T5079	T5077	punct	", ",Launcher
R3097	T5080	T5077	conj	ProfileScan,Launcher
R3098	T5081	T5080	punct	", ",ProfileScan
R3099	T5082	T5083	compound	sequence,search
R3100	T5083	T5080	conj	search,ProfileScan
R3101	T5084	T5083	compound	motif,search
R3102	T5085	T5083	punct	", ",search
R3103	T5086	T5083	conj	ExPASy,search
R3104	T5087	T5086	cc	and,ExPASy
R3105	T5088	T5086	conj	3Dpssm,ExPASy
R3106	T5089	T5074	auxpass	were,used
R3107	T5090	T5074	prep	for,used
R3108	T5091	T5090	pcomp	searching,for
R3109	T5092	T5093	compound	sequence,features
R3110	T5093	T5091	dobj	features,searching
R3111	T5094	T5093	prep	of,features
R3112	T5095	T5096	amod	known,protein
R3113	T5096	T5094	pobj	protein,of
R3114	T5097	T5074	punct	.,used
R3115	T5099	T5100	amod	Phylogenetic,tree
R3116	T5100	T5101	nsubjpass	tree,constructed
R3117	T5102	T5101	auxpass	was,constructed
R3118	T5103	T5101	agent	by,constructed
R3119	T5104	T5105	compound	Clustalw,program
R3120	T5105	T5103	pobj	program,by
R3121	T5106	T5107	punct	(,version
R3122	T5107	T5105	parataxis	version,program
R3123	T5108	T5107	nummod	1.81,version
R3124	T5109	T5107	punct	),version
R3125	T5110	T5101	advcl	using,constructed
R3126	T5111	T5112	nmod	UPGMA,algorithm
R3127	T5112	T5110	dobj	algorithm,using
R3128	T5113	T5111	punct	(,UPGMA
R3129	T5114	T5115	amod	Unweighted,Method
R3130	T5115	T5111	appos	Method,UPGMA
R3131	T5116	T5115	compound	Pair,Method
R3132	T5117	T5115	compound	Group,Method
R3133	T5118	T5115	acl	using,Method
R3134	T5119	T5120	compound	Arithmetic,averages
R3135	T5120	T5118	dobj	averages,using
R3136	T5121	T5112	punct	),algorithm
R3137	T5122	T5123	punct	[,14
R3138	T5123	T5101	parataxis	14,constructed
R3139	T5124	T5123	punct	],14
R3140	T5125	T5101	punct	.,constructed
R3141	T5264	T5265	amod	Neuronal,preparation
R3142	T5266	T5265	compound	cell,preparation
R3143	T5267	T5265	cc	and,preparation
R3144	T5268	T5265	conj	immunostanining,preparation
R3145	T5270	T5271	amod	Hippocampal,cultures
R3146	T5271	T5273	nsubjpass	cultures,prepared
R3147	T5272	T5271	compound	neuron,cultures
R3148	T5274	T5273	auxpass	were,prepared
R3149	T5275	T5276	mark	as,reported
R3150	T5276	T5273	advcl	reported,prepared
R3151	T5277	T5276	advmod	previously,reported
R3152	T5278	T5279	punct	[,6
R3153	T5279	T5273	parataxis	6,prepared
R3154	T5280	T5279	punct	],6
R3155	T5281	T5273	punct	.,prepared
R3156	T5283	T5284	prep	In,dissected
R3157	T5285	T5283	pobj	brief,In
R3158	T5286	T5284	punct	", ",dissected
R3159	T5287	T5288	det	the,hippocampuses
R3160	T5288	T5284	nsubjpass	hippocampuses,dissected
R3161	T5289	T5284	auxpass	were,dissected
R3162	T5290	T5284	prt	out,dissected
R3163	T5291	T5284	prep	from,dissected
R3164	T5292	T5293	compound	mouse,embryos
R3165	T5293	T5291	pobj	embryos,from
R3166	T5294	T5284	prep	at,dissected
R3167	T5295	T5296	nummod	16,days
R3168	T5296	T5294	pobj	days,at
R3169	T5297	T5298	advmod	in,utero
R3170	T5298	T5296	advmod	utero,days
R3171	T5299	T5284	punct	.,dissected
R3172	T5301	T5302	det	The,tissues
R3173	T5302	T5303	nsubjpass	tissues,incubated
R3174	T5304	T5303	auxpass	were,incubated
R3175	T5305	T5303	advmod	then,incubated
R3176	T5306	T5303	prep	for,incubated
R3177	T5307	T5308	nummod	20,min
R3178	T5308	T5306	pobj	min,for
R3179	T5309	T5303	prep	at,incubated
R3180	T5310	T5311	nummod	37,°C
R3181	T5311	T5309	pobj	°C,at
R3182	T5312	T5303	prep	in,incubated
R3183	T5313	T5312	pobj	MEM,in
R3184	T5314	T5313	punct	(,MEM
R3185	T5315	T5316	amod	minimum,medium
R3186	T5316	T5313	appos	medium,MEM
R3187	T5317	T5316	amod	essential,medium
R3188	T5318	T5313	punct	),MEM
R3189	T5319	T5313	acl	modified,MEM
R3190	T5320	T5319	prep	for,modified
R3191	T5321	T5322	compound	suspension,culture
R3192	T5322	T5320	pobj	culture,for
R3193	T5323	T5324	punct	(,Technologies
R3194	T5324	T5313	parataxis	Technologies,MEM
R3195	T5325	T5324	compound	Life,Technologies
R3196	T5326	T5324	punct	),Technologies
R3197	T5327	T5313	cc	plus,MEM
R3198	T5328	T5329	nummod	0.25,%
R3199	T5329	T5330	compound	%,trypsin
R3200	T5330	T5313	conj	trypsin,MEM
R3201	T5331	T5332	punct	(,Technologies
R3202	T5332	T5330	parataxis	Technologies,trypsin
R3203	T5333	T5332	compound	Life,Technologies
R3204	T5334	T5332	punct	),Technologies
R3205	T5335	T5303	punct	.,incubated
R3206	T5337	T5338	det	The,neurons
R3207	T5338	T5341	nsubjpass	neurons,plated
R3208	T5339	T5338	amod	dissociated,neurons
R3209	T5340	T5338	amod	hippocampal,neurons
R3210	T5342	T5341	auxpass	were,plated
R3211	T5343	T5341	prep	on,plated
R3212	T5344	T5345	compound	glass,coverslips
R3213	T5345	T5343	pobj	coverslips,on
R3214	T5346	T5345	acl	coated,coverslips
R3215	T5347	T5346	prep	with,coated
R3216	T5348	T5349	det	a,monolayer
R3217	T5349	T5347	pobj	monolayer,with
R3218	T5350	T5349	amod	confluent,monolayer
R3219	T5351	T5349	prep	of,monolayer
R3220	T5352	T5353	nmod	mouse,astrocytes
R3221	T5353	T5351	pobj	astrocytes,of
R3222	T5354	T5353	amod	cortical,astrocytes
R3223	T5355	T5353	acl	obtained,astrocytes
R3224	T5356	T5357	mark	as,described
R3225	T5357	T5355	advcl	described,obtained
R3226	T5358	T5357	advmod	below,described
R3227	T5359	T5341	punct	.,plated
R3228	T5361	T5362	det	The,neurons
R3229	T5362	T5363	nsubjpass	neurons,maintained
R3230	T5364	T5363	auxpass	were,maintained
R3231	T5365	T5363	prep	at,maintained
R3232	T5366	T5367	nummod	37,°C
R3233	T5367	T5365	pobj	°C,at
R3234	T5368	T5363	prep	in,maintained
R3235	T5369	T5370	det	a,atmosphere
R3236	T5370	T5368	pobj	atmosphere,in
R3237	T5371	T5370	amod	humidified,atmosphere
R3238	T5372	T5370	prep	with,atmosphere
R3239	T5373	T5374	nummod	5,%
R3240	T5374	T5375	compound	%,CO2
R3241	T5375	T5372	pobj	CO2,with
R3242	T5376	T5363	punct	.,maintained
R3243	T5378	T5379	amod	Cortical,astrocytes
R3244	T5379	T5380	nsubjpass	astrocytes,grown
R3245	T5381	T5379	acl	dissociated,astrocytes
R3246	T5382	T5381	prep	from,dissociated
R3247	T5383	T5384	amod	newborn,mouse
R3248	T5384	T5385	compound	mouse,cortices
R3249	T5385	T5382	pobj	cortices,from
R3250	T5386	T5380	auxpass	were,grown
R3251	T5387	T5380	prep	in,grown
R3252	T5388	T5389	compound	culture,flasks
R3253	T5389	T5387	pobj	flasks,in
R3254	T5390	T5380	prep	at,grown
R3255	T5391	T5392	nummod	37,°C
R3256	T5392	T5390	pobj	°C,at
R3257	T5393	T5380	prep	in,grown
R3258	T5394	T5395	det	a,atmosphere
R3259	T5395	T5393	pobj	atmosphere,in
R3260	T5396	T5395	amod	humidified,atmosphere
R3261	T5397	T5395	prep	with,atmosphere
R3262	T5398	T5399	nummod	5,%
R3263	T5399	T5400	compound	%,CO2
R3264	T5400	T5397	pobj	CO2,with
R3265	T5401	T5380	prep	until,grown
R3266	T5402	T5401	pcomp	confluent,until
R3267	T5403	T5380	punct	.,grown
R3268	T5405	T5406	det	The,cells
R3269	T5406	T5407	nsubjpass	cells,exposed
R3270	T5408	T5407	auxpass	were,exposed
R3271	T5409	T5407	prep	to,exposed
R3272	T5410	T5411	quantmod	10,5
R3273	T5411	T5413	nummod	5,M
R3274	T5412	T5411	punct	-,5
R3275	T5413	T5414	compound	M,arabinoside
R3276	T5414	T5409	pobj	arabinoside,to
R3277	T5415	T5414	compound	cytosine,arabinoside
R3278	T5416	T5417	punct	(,Sigma
R3279	T5417	T5414	parataxis	Sigma,arabinoside
R3280	T5418	T5417	punct	),Sigma
R3281	T5419	T5407	cc	and,exposed
R3282	T5420	T5407	conj	cultured,exposed
R3283	T5421	T5420	prep	for,cultured
R3284	T5422	T5423	amod	additional,hrs
R3285	T5423	T5421	pobj	hrs,for
R3286	T5424	T5425	quantmod	12,24
R3287	T5425	T5423	nummod	24,hrs
R3288	T5426	T5425	punct	–,24
R3289	T5427	T5420	prep	at,cultured
R3290	T5428	T5429	nummod	37,°C
R3291	T5429	T5427	pobj	°C,at
R3292	T5430	T5407	punct	.,exposed
R3293	T5432	T5433	prep	After,used
R3294	T5434	T5432	pobj	remove,After
R3295	T5435	T5434	prep	of,remove
R3296	T5436	T5437	det	the,media
R3297	T5437	T5435	pobj	media,of
R3298	T5438	T5434	prep	with,remove
R3299	T5439	T5440	amod	cellular,debris
R3300	T5440	T5438	pobj	debris,with
R3301	T5441	T5433	punct	", ",used
R3302	T5442	T5443	det	the,cells
R3303	T5443	T5433	nsubjpass	cells,used
R3304	T5444	T5433	auxpass	were,used
R3305	T5445	T5433	prep	for,used
R3306	T5446	T5445	pcomp	coating,for
R3307	T5447	T5446	dobj	coverslips,coating
R3308	T5448	T5433	punct	.,used
R3309	T5450	T5451	prep	For,fixed
R3310	T5452	T5450	pobj	immunostaining,For
R3311	T5453	T5451	punct	", ",fixed
R3312	T5454	T5455	amod	neuronal,cells
R3313	T5455	T5451	nsubjpass	cells,fixed
R3314	T5456	T5455	prep	on,cells
R3315	T5457	T5458	det	the,coverslips
R3316	T5458	T5456	pobj	coverslips,on
R3317	T5459	T5451	auxpass	were,fixed
R3318	T5460	T5451	advmod	first,fixed
R3319	T5461	T5451	prep	in,fixed
R3320	T5462	T5461	pobj	PBS,in
R3321	T5463	T5462	acl	containing,PBS
R3322	T5464	T5465	nummod	4,%
R3323	T5465	T5466	compound	%,paraformaldehyde
R3324	T5466	T5463	dobj	paraformaldehyde,containing
R3325	T5467	T5466	punct	(,paraformaldehyde
R3326	T5468	T5466	appos	PFA,paraformaldehyde
R3327	T5469	T5463	punct	),containing
R3328	T5470	T5463	prep	for,containing
R3329	T5471	T5472	nummod	12,hrs
R3330	T5472	T5470	pobj	hrs,for
R3331	T5473	T5463	prep	at,containing
R3332	T5474	T5475	nummod	4,°C
R3333	T5475	T5473	pobj	°C,at
R3334	T5476	T5451	cc	and,fixed
R3335	T5477	T5478	advmod	then,incubated
R3336	T5478	T5451	conj	incubated,fixed
R3337	T5479	T5478	prep	in,incubated
R3338	T5480	T5481	det	a,solution
R3339	T5481	T5479	pobj	solution,in
R3340	T5482	T5481	acl	containing,solution
R3341	T5483	T5484	nummod	4,%
R3342	T5484	T5485	compound	%,PFA
R3343	T5485	T5482	dobj	PFA,containing
R3344	T5486	T5485	cc	and,PFA
R3345	T5487	T5488	nummod	0.4,%
R3346	T5488	T5489	compound	%,100
R3347	T5489	T5485	conj	100,PFA
R3348	T5490	T5489	compound	Triton,100
R3349	T5491	T5489	compound	X,100
R3350	T5492	T5489	punct	-,100
R3351	T5493	T5478	prep	at,incubated
R3352	T5494	T5495	nummod	4,°C
R3353	T5495	T5493	pobj	°C,at
R3354	T5496	T5478	prep	for,incubated
R3355	T5497	T5498	nummod	1,hr
R3356	T5498	T5496	pobj	hr,for
R3357	T5499	T5451	punct	.,fixed
R3358	T5501	T5502	prep	After,incubated
R3359	T5503	T5501	pcomp	washing,After
R3360	T5504	T5503	prep	with,washing
R3361	T5505	T5504	pobj	PBS,with
R3362	T5506	T5507	nummod	three,times
R3363	T5507	T5503	npadvmod	times,washing
R3364	T5508	T5502	punct	", ",incubated
R3365	T5509	T5510	det	the,cells
R3366	T5510	T5502	nsubjpass	cells,incubated
R3367	T5511	T5502	auxpass	were,incubated
R3368	T5512	T5502	prep	with,incubated
R3369	T5513	T5514	det	a,solution
R3370	T5514	T5512	pobj	solution,with
R3371	T5515	T5514	amod	blocking,solution
R3372	T5516	T5514	acl	containing,solution
R3373	T5517	T5518	nummod	1,serum
R3374	T5518	T5516	dobj	serum,containing
R3375	T5519	T5520	punct	:,30
R3376	T5520	T5517	prep	30,1
R3377	T5521	T5518	amod	normal,serum
R3378	T5522	T5518	compound	goat,serum
R3379	T5523	T5502	punct	", ",incubated
R3380	T5524	T5502	cc	and,incubated
R3381	T5525	T5526	advmod	subsequently,incubated
R3382	T5526	T5502	conj	incubated,incubated
R3383	T5527	T5526	prep	with,incubated
R3384	T5528	T5529	det	a,antibody
R3385	T5529	T5527	pobj	antibody,with
R3386	T5530	T5529	nmod	rabbit,antibody
R3387	T5531	T5529	amod	polyclonal,antibody
R3388	T5532	T5529	amod	anti-ACDP,antibody
R3389	T5533	T5534	punct	(,1
R3390	T5534	T5529	parataxis	1,antibody
R3391	T5535	T5536	punct	:,3000
R3392	T5536	T5534	prep	3000,1
R3393	T5537	T5534	punct	),1
R3394	T5538	T5526	advmod	overnight,incubated
R3395	T5539	T5526	prep	at,incubated
R3396	T5540	T5541	nummod	4,°C
R3397	T5541	T5539	pobj	°C,at
R3398	T5542	T5502	punct	.,incubated
R3399	T5544	T5545	prep	After,incubated
R3400	T5546	T5547	amod	extensive,washing
R3401	T5547	T5544	pobj	washing,After
R3402	T5548	T5547	prep	with,washing
R3403	T5549	T5550	nummod	1,%
R3404	T5550	T5551	compound	%,serum
R3405	T5551	T5553	compound	serum,solution
R3406	T5552	T5551	compound	goat,serum
R3407	T5553	T5548	pobj	solution,with
R3408	T5554	T5553	compound	PBS,solution
R3409	T5555	T5545	punct	", ",incubated
R3410	T5556	T5557	det	the,cells
R3411	T5557	T5545	nsubjpass	cells,incubated
R3412	T5558	T5545	auxpass	were,incubated
R3413	T5559	T5545	prep	with,incubated
R3414	T5560	T5561	det	an,antibody
R3415	T5561	T5559	pobj	antibody,with
R3416	T5562	T5561	nmod	Alex,antibody
R3417	T5563	T5562	nummod	488,Alex
R3418	T5564	T5561	amod	conjugated,antibody
R3419	T5565	T5561	amod	secondary,antibody
R3420	T5566	T5567	punct	(,Probes
R3421	T5567	T5561	parataxis	Probes,antibody
R3422	T5568	T5567	dep	1,Probes
R3423	T5569	T5570	punct	:,100
R3424	T5570	T5568	prep	100,1
R3425	T5571	T5568	prep	in,1
R3426	T5572	T5573	nummod	1,%
R3427	T5573	T5574	compound	%,serum
R3428	T5574	T5576	compound	serum,solution
R3429	T5575	T5574	compound	goat,serum
R3430	T5576	T5571	pobj	solution,in
R3431	T5577	T5576	compound	PBS,solution
R3432	T5578	T5567	punct	", ",Probes
R3433	T5579	T5567	compound	Molecular,Probes
R3434	T5580	T5567	punct	),Probes
R3435	T5581	T5545	prep	for,incubated
R3436	T5582	T5583	nummod	3,hrs
R3437	T5583	T5581	pobj	hrs,for
R3438	T5584	T5545	prep	at,incubated
R3439	T5585	T5586	compound	room,temperature
R3440	T5586	T5584	pobj	temperature,at
R3441	T5587	T5545	punct	.,incubated
R3442	T5589	T5590	prep	Following,slipped
R3443	T5591	T5592	amod	final,washes
R3444	T5592	T5589	pobj	washes,Following
R3445	T5593	T5592	prep	with,washes
R3446	T5594	T5595	nummod	1,%
R3447	T5595	T5596	compound	%,serum
R3448	T5596	T5598	compound	serum,solution
R3449	T5597	T5596	compound	goat,serum
R3450	T5598	T5593	pobj	solution,with
R3451	T5599	T5598	compound	PBS,solution
R3452	T5600	T5590	punct	", ",slipped
R3453	T5601	T5602	det	the,cells
R3454	T5602	T5590	nsubjpass	cells,slipped
R3455	T5603	T5602	amod	neuronal,cells
R3456	T5604	T5602	prep	on,cells
R3457	T5605	T5606	det	the,coverslips
R3458	T5606	T5604	pobj	coverslips,on
R3459	T5607	T5590	auxpass	were,slipped
R3460	T5608	T5590	dep	cover,slipped
R3461	T5609	T5590	punct	-,slipped
R3462	T5610	T5590	prep	with,slipped
R3463	T5611	T5612	det	a,medium
R3464	T5612	T5610	pobj	medium,with
R3465	T5613	T5614	npadvmod	glycerol,based
R3466	T5614	T5612	amod	based,medium
R3467	T5615	T5614	punct	-,based
R3468	T5616	T5612	amod	anti-photobleach,medium
R3469	T5617	T5590	punct	.,slipped
R3470	T5619	T5620	det	The,cells
R3471	T5620	T5621	nsubjpass	cells,viewed
R3472	T5622	T5621	auxpass	were,viewed
R3473	T5623	T5621	prep	under,viewed
R3474	T5624	T5625	det	a,microscope
R3475	T5625	T5623	pobj	microscope,under
R3476	T5626	T5625	amod	confocal,microscope
R3477	T5627	T5628	punct	(,Zeiss
R3478	T5628	T5625	parataxis	Zeiss,microscope
R3479	T5629	T5628	compound	Carl,Zeiss
R3480	T5630	T5628	punct	),Zeiss
R3481	T5631	T5621	punct	.,viewed
R3482	T5633	T5634	nsubjpass	Images,captured
R3483	T5635	T5634	auxpass	were,captured
R3484	T5636	T5634	prep	with,captured
R3485	T5637	T5638	det	a,camera
R3486	T5638	T5636	pobj	camera,with
R3487	T5639	T5638	compound	CCD,camera
R3488	T5640	T5634	cc	and,captured
R3489	T5641	T5634	conj	acquired,captured
R3490	T5642	T5641	prep	by,acquired
R3491	T5643	T5644	det	the,software
R3492	T5644	T5642	pobj	software,by
R3493	T5645	T5644	compound	Scion,software
R3494	T5646	T5644	compound	Image,software
R3495	T5647	T5648	punct	(,Corporation
R3496	T5648	T5644	parataxis	Corporation,software
R3497	T5649	T5648	compound	Scion,Corporation
R3498	T5650	T5648	punct	", ",Corporation
R3499	T5651	T5648	npadvmod	Frederick,Corporation
R3500	T5652	T5648	punct	", ",Corporation
R3501	T5653	T5648	npadvmod	MD,Corporation
R3502	T5654	T5648	punct	),Corporation
R3503	T5655	T5634	punct	.,captured
R3504	T5677	T5678	compound	Gene,bank
R3505	T5678	T5679	compound	bank,number
R3506	T5680	T5679	compound	accession,number
R3507	T5682	T5683	det	The,sequences
R3508	T5683	T5685	nsubjpass	sequences,deposited
R3509	T5684	T5683	compound	cDNA,sequences
R3510	T5686	T5683	prep	for,sequences
R3511	T5687	T5688	det	the,family
R3512	T5688	T5686	pobj	family,for
R3513	T5689	T5688	compound	Acdp,family
R3514	T5690	T5688	compound	gene,family
R3515	T5691	T5685	aux	have,deposited
R3516	T5692	T5685	advmod	already,deposited
R3517	T5693	T5685	auxpass	been,deposited
R3518	T5694	T5685	prep	in,deposited
R3519	T5695	T5696	compound	gene,bank
R3520	T5696	T5694	pobj	bank,in
R3521	T5697	T5685	prep	with,deposited
R3522	T5698	T5699	compound	accession,AF202994
R3523	T5699	T5697	pobj	AF202994,with
R3524	T5700	T5699	compound	numbers,AF202994
R3525	T5701	T5699	punct	(,AF202994
R3526	T5702	T5699	appos	Acdp1,AF202994
R3527	T5703	T5699	punct	),AF202994
R3528	T5704	T5699	punct	", ",AF202994
R3529	T5705	T5699	conj	AF216961,AF202994
R3530	T5706	T5705	punct	(,AF216961
R3531	T5707	T5705	appos	Acdp2,AF216961
R3532	T5708	T5705	punct	),AF216961
R3533	T5709	T5705	punct	", ",AF216961
R3534	T5710	T5705	conj	AF216964,AF216961
R3535	T5711	T5710	punct	(,AF216964
R3536	T5712	T5710	appos	Acdp3,AF216964
R3537	T5713	T5710	punct	),AF216964
R3538	T5714	T5710	cc	and,AF216964
R3539	T5715	T5710	conj	AF216963,AF216964
R3540	T5716	T5715	punct	(,AF216963
R3541	T5717	T5715	appos	Acdp4,AF216963
R3542	T5718	T5699	punct	),AF202994
R3543	T5719	T5685	punct	.,deposited

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T5666	5437-5442	PR:000005646	denotes	Acdp4
T5665	5416-5421	PR:000005645	denotes	Acdp3
T5664	5398-5403	PR:000005644	denotes	Acdp2
T5663	5380-5385	PR:000005643	denotes	Acdp1
T5662	5337-5341	SO:0000704	denotes	gene
T5661	5294-5298	SO:0000704	denotes	gene
T5660	5235-5239	SO:0000704	denotes	Gene
T5207	5014-5022	CHEBI:17754	denotes	glycerol
T5206	4955-4969	CL:0000540	denotes	neuronal cells
T5205	4941-4949	CHEBI:75958	denotes	solution
T5204	4926-4930	NCBITaxon:9925	denotes	goat
T5203	4836-4844	CHEBI:75958	denotes	solution
T5202	4821-4825	NCBITaxon:9925	denotes	goat
T5201	4799-4807	GO:0042571	denotes	antibody
T5200	4778-4788	MOP:0000779	denotes	conjugated
T5199	4769-4777	CHEBI:52661	denotes	Alex 488
T5198	4726-4734	CHEBI:75958	denotes	solution
T5197	4711-4715	NCBITaxon:9925	denotes	goat
T5196	4643-4651	GO:0042571	denotes	antibody
T5195	4615-4621	NCBITaxon:9986	denotes	rabbit
T5194	4569-4573	NCBITaxon:9925	denotes	goat
T5193	4537-4545	CHEBI:75958	denotes	solution
T5192	4430-4442	CHEBI:9750	denotes	Triton X-100
T5191	4394-4402	CHEBI:75958	denotes	solution
T5190	4258-4272	CL:0000540	denotes	neuronal cells
T5189	4070-4090	CHEBI:28680	denotes	cytosine arabinoside
T5188	4016-4019	CHEBI:16526	denotes	CO2
T5187	3949-3954	GO:0040007	denotes	grown
T5186	3935-3943	UBERON:0001851	denotes	cortices
T5185	3929-3934	NCBITaxon:10088	denotes	mouse
T5184	3884-3903	CL:0002605	denotes	Cortical astrocytes
T5183	3884-3892	UBERON:0001851	denotes	Cortical
T5182	3879-3882	CHEBI:16526	denotes	CO2
T5181	3812-3819	CL:0000540	denotes	neurons
T5180	3759-3778	CL:0002605	denotes	cortical astrocytes
T5179	3759-3767	UBERON:0001851	denotes	cortical
T5178	3753-3758	NCBITaxon:10088	denotes	mouse
T5177	3664-3683	CL:0002608	denotes	hippocampal neurons
T5176	3619-3626	PR:000027795	denotes	trypsin
T5175	3475-3482	UBERON:0000479	denotes	tissues
T5174	3442-3449	UBERON:0000922	denotes	embryos
T5173	3436-3441	NCBITaxon:10088	denotes	mouse
T5172	3314-3332	CL:0002608	denotes	Hippocampal neuron
T5171	3268-3281	CL:0000540	denotes	Neuronal cell
T5009	3077-3094	SO:0000110	denotes	sequence features
T5008	2767-2775	SO:0000857	denotes	homology
T5007	2733-2737	SO:0000704	denotes	Gene
T4928	2611-2616	NCBITaxon:10088	denotes	Mouse
T4927	2553-2559	SO:0001026	denotes	genome
T4926	2547-2552	NCBITaxon:10088	denotes	mouse
T4925	2450-2456	SO:0000205	denotes	3' UTR
T4924	2437-2444	SO:0000112	denotes	Primers
T4923	2324-2329	NCBITaxon:10088	denotes	mouse
T4805	2211-2221	GO:0042571	denotes	antibodies
T4804	1992-1997	PR:000005643	denotes	Acdp1
T4803	1964-1974	GO:0042571	denotes	antibodies
T4802	1923-1929	SO:0000417	denotes	domain
T4801	1880-1885	PR:000005643	denotes	Acdp1
T4800	1856-1862	NCBITaxon:6462	denotes	limpet
T4799	1833-1839	MOP:0000779	denotes	linked
T4798	1778-1786	GO:0042571	denotes	Antibody
T4627	1678-1681	CHEBI:8984	denotes	SDS
T4626	1592-1595	CHEBI:8984	denotes	SDS
T4625	1484-1498	GO:0097617	denotes	Hybridizations
T4624	1439-1445	SO:0000112	denotes	primer
T4623	1413-1416	CHEBI:37972	denotes	32P
T4622	1380-1390	GO:0006412	denotes	translated
T4621	1375-1397	SO:0000205	denotes	3' untranslated region
T4620	1362-1366	SO:0000147	denotes	exon
T4619	1348-1350	SO:0000028	denotes	bp
T4618	1311-1315	SO:0000704	denotes	gene
T4617	1236-1243	UBERON:0000479	denotes	tissues
T4616	1230-1235	NCBITaxon:10088	denotes	mouse
T4332	1124-1127	SO:0000153	denotes	BAC
T4331	1090-1096	SO:0000204	denotes	5' UTR
T4330	1075-1079	SO:0000147	denotes	exon
T4329	968-982	SO:0000132	denotes	reverse primer
T4328	916-930	SO:0000121	denotes	forward primer
T4327	813-820	CHEBI:2511	denotes	agarose
T4326	773-785	SO:0000006	denotes	PCR products
T4325	730-739	GO:0097617	denotes	annealing
T4324	709-721	SO:0000006	denotes	PCR products
T4323	683-687	SO:0000704	denotes	gene
T4322	677-682	NCBITaxon:10088	denotes	mouse
T4321	617-631	SO:0000121	denotes	forward primer
T4320	611-616	NCBITaxon:9606	denotes	human
T4319	585-588	SO:0000345	denotes	EST
T4318	579-584	NCBITaxon:10088	denotes	mouse
T4317	555-569	SO:0000132	denotes	reverse primer
T4316	493-502	GO:0097617	denotes	annealing
T4315	470-475	NCBITaxon:10088	denotes	mouse
T4314	446-455	SO:0000853	denotes	homologue
T4313	410-413	SO:0000345	denotes	EST
T4312	404-409	NCBITaxon:10088	denotes	mouse
T4311	380-394	SO:0000132	denotes	reverse primer
T4310	374-379	NCBITaxon:10088	denotes	mouse
T4309	355-366	SO:0000318	denotes	start codon
T4308	315-320	NCBITaxon:9606	denotes	human
T4307	289-303	SO:0000121	denotes	forward primer
T4306	240-243	SO:0000345	denotes	EST
T4305	223-226	SO:0000345	denotes	EST
T4304	217-222	NCBITaxon:10088	denotes	mouse
T4303	149-154	NCBITaxon:9606	denotes	human
T4302	88-97	SO:0000853	denotes	homologue
T4301	82-87	NCBITaxon:9606	denotes	human
T4300	47-51	SO:0000704	denotes	gene

2_test

Id	Subject	Object	Predicate	Lexical cue
14723793-10049587-10642194	132-133	10049587	denotes	2
14723793-11595174-10642195	1771-1772	11595174	denotes	3
14723793-10550218-10642196	1773-1774	10550218	denotes	4
14723793-11533054-10642197	2046-2047	11533054	denotes	5
14723793-12657465-10642198	2125-2126	12657465	denotes	1
14723793-7984417-10642199	3262-3264	7984417	denotes	14
14723793-8657283-10642200	3380-3381	8657283	denotes	6

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id	Subject	Object	Predicate	Lexical cue
T5676	5437-5442	PR_EXT:000005646	denotes	Acdp4
T5675	5416-5421	PR_EXT:000005645	denotes	Acdp3
T5674	5398-5403	PR_EXT:000005644	denotes	Acdp2
T5673	5380-5385	PR_EXT:000005643	denotes	Acdp1
T5672	5337-5341	SO_EXT:0000704	denotes	gene
T5671	5294-5298	SO_EXT:0000704	denotes	gene
T5670	5289-5293	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T5669	5271-5280	SO_EXT:biological_sequence	denotes	sequences
T5668	5266-5270	SO_EXT:cDNA	denotes	cDNA
T5667	5235-5239	SO_EXT:0000704	denotes	Gene
T5263	5058-5063	CL_GO_EXT:cell	denotes	cells
T5262	5014-5022	CHEBI:17754	denotes	glycerol
T5261	4964-4969	CL_GO_EXT:cell	denotes	cells
T5260	4955-4969	CL:0000540	denotes	neuronal cells
T5259	4941-4949	CHEBI:75958	denotes	solution
T5258	4926-4930	NCBITaxon:9925	denotes	goat
T5257	4856-4862	CHEBI_SO_EXT:molecular_probe	denotes	Probes
T5256	4846-4855	CHEBI_EXT:polyatomic_entity_or_group	denotes	Molecular
T5255	4836-4844	CHEBI:75958	denotes	solution
T5254	4821-4825	NCBITaxon:9925	denotes	goat
T5253	4799-4807	GO:0042571	denotes	antibody
T5252	4778-4788	MOP:0000779	denotes	conjugated
T5251	4769-4777	CHEBI:52661	denotes	Alex 488
T5250	4740-4745	CL_GO_EXT:cell	denotes	cells
T5249	4726-4734	CHEBI:75958	denotes	solution
T5248	4711-4715	NCBITaxon:9925	denotes	goat
T5247	4643-4651	GO:0042571	denotes	antibody
T5246	4638-4642	PR_EXT:ACDP_or_CNNM	denotes	ACDP
T5245	4615-4621	NCBITaxon:9986	denotes	rabbit
T5244	4569-4573	NCBITaxon:9925	denotes	goat
T5243	4537-4545	CHEBI:75958	denotes	solution
T5242	4500-4505	CL_GO_EXT:cell	denotes	cells
T5241	4430-4442	CHEBI:9750	denotes	Triton X-100
T5240	4417-4420	CHEBI_EXT:paraformaldehye	denotes	PFA
T5239	4394-4402	CHEBI:75958	denotes	solution
T5238	4347-4350	CHEBI_EXT:paraformaldehye	denotes	PFA
T5237	4329-4345	CHEBI_EXT:paraformaldehye	denotes	paraformaldehyde
T5236	4267-4272	CL_GO_EXT:cell	denotes	cells
T5235	4258-4272	CL:0000540	denotes	neuronal cells
T5234	4198-4203	CL_GO_EXT:cell	denotes	cells
T5233	4177-4185	CL_GO_EXT:cell	denotes	cellular
T5232	4070-4090	CHEBI:28680	denotes	cytosine arabinoside
T5231	4041-4046	CL_GO_EXT:cell	denotes	cells
T5230	4016-4019	CHEBI:16526	denotes	CO2
T5229	3949-3954	GO:0040007	denotes	grown
T5228	3935-3943	UBERON:0001851	denotes	cortices
T5227	3929-3934	NCBITaxon:10088	denotes	mouse
T5226	3921-3928	PATO_UBERON_EXT:neonate_or_newborn	denotes	newborn
T5225	3884-3903	CL:0002605	denotes	Cortical astrocytes
T5224	3884-3892	UBERON:0001851	denotes	Cortical
T5223	3879-3882	CHEBI:16526	denotes	CO2
T5222	3812-3819	CL:0000540	denotes	neurons
T5221	3759-3778	CL:0002605	denotes	cortical astrocytes
T5220	3759-3767	UBERON:0001851	denotes	cortical
T5219	3753-3758	NCBITaxon:10088	denotes	mouse
T5218	3664-3683	CL:0002608	denotes	hippocampal neurons
T5217	3664-3675	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampal
T5216	3619-3626	PR_EXT:000027795	denotes	trypsin
T5215	3475-3482	UBERON:0000479	denotes	tissues
T5214	3442-3449	UBERON:0000922	denotes	embryos
T5213	3436-3441	NCBITaxon:10088	denotes	mouse
T5212	3398-3411	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	hippocampuses
T5211	3314-3332	CL:0002608	denotes	Hippocampal neuron
T5210	3314-3325	UBERON_EXT:hippocampus_proper_or_hippocampal_formation	denotes	Hippocampal
T5209	3277-3281	CL_GO_EXT:cell	denotes	cell
T5208	3268-3281	CL:0000540	denotes	Neuronal cell
T5022	3104-3111	CHEBI_PR_EXT:protein	denotes	protein
T5021	3077-3094	SO_EXT:0000110	denotes	sequence features
T5020	3018-3023	SO_EXT:sequence_or_structure_motif	denotes	motif
T5019	3009-3017	SO_EXT:biological_sequence	denotes	sequence
T5018	2924-2932	SO_EXT:biological_sequence	denotes	sequence
T5017	2863-2871	SO_EXT:biological_sequence	denotes	sequence
T5016	2767-2775	SO:0000857	denotes	homology
T5015	2763-2766	CHEBI_SO_EXT:DNA	denotes	DNA
T5014	2751-2758	CHEBI_PR_EXT:protein	denotes	Protein
T5013	2733-2737	SO_EXT:0000704	denotes	Gene
T5012	2685-2693	SO_EXT:sequence_assembly_entity_or_process	denotes	assembly
T5011	2676-2684	SO_EXT:biological_sequence	denotes	Sequence
T5010	2658-2666	SO_EXT:biological_sequence	denotes	Sequence
T4940	2611-2616	NCBITaxon:10088	denotes	Mouse
T4939	2553-2559	SO_EXT:0001026	denotes	genome
T4938	2547-2552	NCBITaxon:10088	denotes	mouse
T4937	2523-2529	SO_EXT:sequence_cloned_entity	denotes	clones
T4936	2465-2469	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T4935	2450-2456	SO_EXT:0000205	denotes	3' UTR
T4934	2437-2444	SO_EXT:0000112	denotes	Primers
T4933	2396-2415	GO_SO_EXT:chromosomal_part_or_position_or_region_or_site	denotes	chromosome location
T4932	2367-2375	OBI_SO_EXT:gene_or_genetic_investigation_or_genetic_process	denotes	Genetics
T4931	2324-2329	NCBITaxon:10088	denotes	mouse
T4930	2303-2315	GO_PATO_EXT:biological_localization_process_or_quality	denotes	localization
T4929	2292-2302	GO_SO_EXT:chromosome	denotes	Chromosome
T4814	1886-1888,1895-1904	CHEBI_SO_EXT:N_terminus_or_N_terminal_region	denotes	N- terminals
T4812	1880-1885	PR_EXT:000005643	denotes	Acdp1
T4811	1856-1862	NCBITaxon:6462	denotes	limpet
T4810	1833-1839	MOP:0000779	denotes	linked
T4809	1833-1839	SO_EXT:sequence_fusion_process	denotes	linked
T4808	1824-1832	CHEBI_SO_EXT:peptide_or_peptide_region	denotes	Peptides
T4807	1778-1797	GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production	denotes	Antibody production
T4806	1778-1786	GO:0042571	denotes	Antibody
T4646	1678-1681	CHEBI:8984	denotes	SDS
T4645	1668-1671	CHEBI_EXT:saline_sodium_citrate	denotes	SSC
T4644	1652-1658	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T4643	1592-1595	CHEBI:8984	denotes	SDS
T4642	1582-1585	CHEBI_EXT:saline_sodium_citrate	denotes	SSC
T4641	1570-1576	CHEBI_CHMO_EXT:buffer_solution	denotes	buffer
T4640	1484-1498	GO:0097617	denotes	Hybridizations
T4639	1439-1445	SO_EXT:0000112	denotes	primer
T4638	1417-1421	CHEBI_EXT:dCTP	denotes	dCTP
T4637	1413-1416	CHEBI:37972	denotes	32P
T4636	1398-1405	CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process	denotes	labeled
T4635	1380-1390	GO:0006412	denotes	translated
T4634	1375-1397	SO_EXT:0000205	denotes	3' untranslated region
T4633	1362-1366	SO_EXT:0000147	denotes	exon
T4632	1348-1350	SO_EXT:0000028	denotes	bp
T4631	1311-1315	SO_EXT:0000704	denotes	gene
T4630	1306-1310	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T4629	1236-1243	UBERON:0000479	denotes	tissues
T4628	1230-1235	NCBITaxon:10088	denotes	mouse
T4822	2211-2221	GO:0042571	denotes	antibodies
T4821	2006-2010	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T4820	1992-1997	PR_EXT:000005643	denotes	Acdp1
T4819	1964-1974	GO:0042571	denotes	antibodies
T4818	1950-1974	GO_EXT:antibody_or_immunoglobulin_biosynthesis_or_production	denotes	generation of antibodies
T4817	1923-1929	SO_EXT:0000417	denotes	domain
T4816	1913-1922	SO_EXT:biological_conservation_process_or_quality	denotes	conserved
T4815	1893-1904	CHEBI_SO_EXT:C_terminus_or_C_terminal_region	denotes	C-terminals
T4391	1128-1131	CHEBI_SO_EXT:DNA	denotes	DNA
T4390	1124-1127	SO_EXT:0000153	denotes	BAC
T4389	1097-1106	SO_EXT:biological_sequence	denotes	sequences
T4388	1090-1096	SO_EXT:0000204	denotes	5' UTR
T4387	1075-1079	SO_EXT:0000147	denotes	exon
T4386	1063-1071	SO_EXT:biological_sequence	denotes	sequence
T4385	1021-1037	SO_EXT:coding_sequence	denotes	coding sequences
T4384	994-1002	SO_EXT:biological_sequence	denotes	sequence
T4383	968-982	SO_EXT:0000132	denotes	reverse primer
T4382	953-961	SO_EXT:biological_sequence	denotes	sequence
T4381	916-930	SO_EXT:0000121	denotes	forward primer
T4380	877-885	SO_EXT:biological_sequence	denotes	sequence
T4379	813-820	CHEBI:2511	denotes	agarose
T4378	773-785	SO_EXT:0000006	denotes	PCR products
T4377	730-739	GO:0097617	denotes	annealing
T4376	709-721	SO_EXT:0000006	denotes	PCR products
T4375	683-687	SO_EXT:0000704	denotes	gene
T4374	677-682	NCBITaxon:10088	denotes	mouse
T4373	617-631	SO_EXT:0000121	denotes	forward primer
T4372	611-616	NCBITaxon:9606	denotes	human
T4371	589-597	SO_EXT:biological_sequence	denotes	sequence
T4370	585-588	SO_EXT:0000345	denotes	EST
T4369	579-584	NCBITaxon:10088	denotes	mouse
T4368	555-569	SO_EXT:0000132	denotes	reverse primer
T4367	493-502	GO:0097617	denotes	annealing
T4366	476-480	SO_EXT:cDNA	denotes	cDNA
T4365	470-475	NCBITaxon:10088	denotes	mouse
T4364	456-464	SO_EXT:biological_sequence	denotes	sequence
T4363	446-455	SO_EXT:0000853	denotes	homologue
T4362	414-420	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	marker
T4361	410-413	SO_EXT:0000345	denotes	EST
T4360	404-409	NCBITaxon:10088	denotes	mouse
T4359	380-394	SO_EXT:0000132	denotes	reverse primer
T4358	374-379	NCBITaxon:10088	denotes	mouse
T4357	355-366	SO_EXT:0000318	denotes	start codon
T4356	334-347	SO_EXT:coding_sequence	denotes	coding region
T4355	329-333	SO_EXT:cDNA	denotes	cDNA
T4354	321-325	PR_EXT:ACDP_or_CNNM	denotes	ACDP
T4353	315-320	NCBITaxon:9606	denotes	human
T4352	289-303	SO_EXT:0000121	denotes	forward primer
T4351	274-278	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T4350	244-251	CHEBI_SO_EXT:molecular_indicator_or_label_or_marker_or_tag	denotes	markers
T4349	240-243	SO_EXT:0000345	denotes	EST
T4348	223-226	SO_EXT:0000345	denotes	EST
T4347	217-222	NCBITaxon:10088	denotes	mouse
T4346	183-192	SO_EXT:biological_sequence	denotes	sequences
T4345	180-182	CHEBI_SO_EXT:amino_acid	denotes	AA
T4344	160-165	SO_EXT:cDNA	denotes	cDNAs
T4343	155-159	PR_EXT:ACDP_or_CNNM	denotes	ACDP
T4342	149-154	NCBITaxon:9606	denotes	human
T4341	98-107	SO_EXT:biological_sequence	denotes	sequences
T4340	88-97	SO_EXT:0000853	denotes	homologue
T4339	82-87	NCBITaxon:9606	denotes	human
T4338	47-51	SO_EXT:0000704	denotes	gene
T4337	42-46	PR_EXT:ACDP_or_CNNM	denotes	Acdp
T4336	27-34	SO_EXT:sequence_cloning_process	denotes	cloning
T4335	22-26	SO_EXT:cDNA	denotes	cDNA
T4334	14-21	SO_EXT:sequence_cloning_process	denotes	cloning
T4333	9-13	SO_EXT:cDNA	denotes	cDNA

PMC:340383 / 17882-23326 JSONTXT

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craft-ca-core-ex-dev

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