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Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos. The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes. Immunostaining was using the Acdp1 C-terminus specific antibody. Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane. A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins."}

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T2861","span":{"begin":782,"end":790},"obj":"CHEBI_PR_EXT:protein"},{"id":"T2860","span":{"begin":777,"end":781},"obj":"PR_EXT:ACDP_or_CNNM"},{"id":"T2859","span":{"begin":758,"end":765},"obj":"SO_EXT:0000417"},{"id":"T2858","span":{"begin":749,"end":757},"obj":"GO:0016020"},{"id":"T2857","span":{"begin":667,"end":672},"obj":"PR_EXT:000005643"},{"id":"T2856","span":{"begin":646,"end":654},"obj":"GO:0016020"},{"id":"T2855","span":{"begin":591,"end":595},"obj":"CL_GO_EXT:cell"},{"id":"T2854","span":{"begin":548,"end":563},"obj":"GO:0005886"},{"id":"T2853","span":{"begin":531,"end":540},"obj":"GO_PATO_EXT:biological_localization_process_or_quality"},{"id":"T2852","span":{"begin":508,"end":513},"obj":"PR_EXT:000005643"},{"id":"T2851","span":{"begin":467,"end":475},"obj":"GO:0042571"},{"id":"T2850","span":{"begin":447,"end":457},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T2849","span":{"begin":441,"end":446},"obj":"PR_EXT:000005643"},{"id":"T2848","span":{"begin":381,"end":400},"obj":"CL:0002605"},{"id":"T2847","span":{"begin":381,"end":389},"obj":"UBERON:0001851"},{"id":"T2846","span":{"begin":375,"end":380},"obj":"NCBITaxon:10088"},{"id":"T2845","span":{"begin":296,"end":303},"obj":"CL:0000540"},{"id":"T2844","span":{"begin":283,"end":290},"obj":"UBERON:0000922"},{"id":"T2843","span":{"begin":277,"end":282},"obj":"NCBITaxon:10088"},{"id":"T2842","span":{"begin":243,"end":262},"obj":"CL:0002608"},{"id":"T2841","span":{"begin":243,"end":254},"obj":"UBERON_EXT:hippocampus_proper_or_hippocampal_formation"},{"id":"T2840","span":{"begin":227,"end":239},"obj":"GO_PATO_EXT:biological_localization_process_or_quality"},{"id":"T2839","span":{"begin":218,"end":226},"obj":"CL_GO_EXT:cell"},{"id":"T2838","span":{"begin":215,"end":226},"obj":"GO_UBERON_EXT:cellular_component_or_cell_part"},{"id":"T2837","span":{"begin":192,"end":197},"obj":"UBERON:0000955"},{"id":"T2836","span":{"begin":179,"end":184},"obj":"PR_EXT:000005643"},{"id":"T2835","span":{"begin":165,"end":175},"obj":"GO:0010467"},{"id":"T2834","span":{"begin":112,"end":117},"obj":"CL_GO_EXT:cell"},{"id":"T2833","span":{"begin":98,"end":104},"obj":"PR_EXT:000005643"},{"id":"T2832","span":{"begin":40,"end":48},"obj":"GO:0042571"},{"id":"T2831","span":{"begin":29,"end":39},"obj":"CHEBI_SO_EXT:C_terminus_or_C_terminal_region"},{"id":"T2830","span":{"begin":23,"end":28},"obj":"PR_EXT:000005643"}],"text":"The specificity of the Acdp1 C-terminus antibody suggests the possibility of using it to localize Acdp-1 within cells. Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos. The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes. Immunostaining was using the Acdp1 C-terminus specific antibody. Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane. A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins."}