Id |
Subject |
Object |
Predicate |
Lexical cue |
T3199 |
199-201 |
PRP |
denotes |
we |
T3198 |
197-199 |
, |
denotes |
, |
T3197 |
192-197 |
NN |
denotes |
brain |
T3196 |
188-191 |
DT |
denotes |
the |
T3195 |
185-187 |
IN |
denotes |
in |
T3194 |
179-184 |
NN |
denotes |
Acdp1 |
T3193 |
176-178 |
IN |
denotes |
of |
T3192 |
165-175 |
NN |
denotes |
expression |
T3191 |
155-164 |
JJ |
denotes |
exclusive |
T3190 |
148-154 |
RB |
denotes |
almost |
T3189 |
202-210 |
VBD |
denotes |
examined |
T3188 |
134-138 |
NN |
denotes |
blot |
T3187 |
125-133 |
NNP |
denotes |
Northern |
T3186 |
139-147 |
VBD |
denotes |
revealed |
T3185 |
119-124 |
IN |
denotes |
Since |
T3184 |
118-291 |
sentence |
denotes |
Since Northern blot revealed almost exclusive expression of Acdp1 in the brain, we examined its subcellular localization in hippocampus neurons isolated from mouse embryos. |
T3183 |
117-118 |
. |
denotes |
. |
T3182 |
112-117 |
NNS |
denotes |
cells |
T3181 |
105-111 |
IN |
denotes |
within |
T3180 |
103-104 |
CD |
denotes |
1 |
T3179 |
102-103 |
HYPH |
denotes |
- |
T3178 |
98-102 |
NN |
denotes |
Acdp |
T3177 |
89-97 |
VB |
denotes |
localize |
T3176 |
86-88 |
TO |
denotes |
to |
T3175 |
83-85 |
PRP |
denotes |
it |
T3174 |
77-82 |
VBG |
denotes |
using |
T3173 |
74-76 |
IN |
denotes |
of |
T3172 |
62-73 |
NN |
denotes |
possibility |
T3171 |
58-61 |
DT |
denotes |
the |
T3170 |
30-31 |
HYPH |
denotes |
- |
T3169 |
31-39 |
NN |
denotes |
terminus |
T3168 |
29-30 |
NN |
denotes |
C |
T3167 |
23-28 |
NN |
denotes |
Acdp1 |
T3166 |
40-48 |
NN |
denotes |
antibody |
T3165 |
19-22 |
DT |
denotes |
the |
T3164 |
16-18 |
IN |
denotes |
of |
T3163 |
49-57 |
VBZ |
denotes |
suggests |
T3162 |
4-15 |
NN |
denotes |
specificity |
T3161 |
0-3 |
DT |
denotes |
The |
T3297 |
790-791 |
. |
denotes |
. |
T3296 |
777-781 |
NN |
denotes |
Acdp |
T3295 |
782-790 |
NN |
denotes |
proteins |
T3294 |
773-776 |
DT |
denotes |
the |
T3293 |
766-772 |
IN |
denotes |
within |
T3292 |
758-765 |
NNS |
denotes |
domains |
T3291 |
744-757 |
NN |
denotes |
transmembrane |
T3290 |
741-743 |
IN |
denotes |
of |
T3289 |
729-740 |
NN |
denotes |
observation |
T3288 |
725-728 |
DT |
denotes |
the |
T3287 |
720-724 |
IN |
denotes |
with |
T3286 |
709-719 |
JJ |
denotes |
consistent |
T3285 |
706-708 |
VBZ |
denotes |
is |
T3284 |
700-705 |
WDT |
denotes |
which |
T3283 |
698-700 |
, |
denotes |
, |
T3282 |
697-698 |
-RRB- |
denotes |
) |
T3281 |
696-697 |
CD |
denotes |
7 |
T3280 |
691-695 |
NN |
denotes |
Fig. |
T3279 |
690-691 |
-LRB- |
denotes |
( |
T3278 |
672-673 |
HYPH |
denotes |
- |
T3277 |
673-689 |
NN |
denotes |
immunoreactivity |
T3276 |
667-672 |
NN |
denotes |
Acdp1 |
T3275 |
664-666 |
IN |
denotes |
of |
T3274 |
655-663 |
NN |
denotes |
location |
T3273 |
646-654 |
NN |
denotes |
membrane |
T3272 |
631-638 |
RB |
denotes |
clearly |
T3271 |
620-630 |
NN |
denotes |
micrometer |
T3270 |
616-619 |
CD |
denotes |
0.5 |
T3269 |
613-615 |
IN |
denotes |
of |
T3268 |
603-612 |
NN |
denotes |
thickness |
T3267 |
599-602 |
DT |
denotes |
the |
T3266 |
596-598 |
IN |
denotes |
at |
T3265 |
591-595 |
NN |
denotes |
cell |
T3264 |
589-590 |
DT |
denotes |
a |
T3263 |
586-588 |
IN |
denotes |
of |
T3262 |
577-585 |
NNS |
denotes |
sections |
T3261 |
574-576 |
IN |
denotes |
of |
T3260 |
639-645 |
VBD |
denotes |
showed |
T3259 |
567-573 |
NN |
denotes |
series |
T3258 |
565-566 |
DT |
denotes |
A |
T3257 |
564-791 |
sentence |
denotes |
A series of sections of a cell at the thickness of 0.5 micrometer clearly showed membrane location of Acdp1-immunoreactivity (Fig. 7), which is consistent with the observation of transmembrane domains within the Acdp proteins. |
T3256 |
563-564 |
. |
denotes |
. |
T3255 |
548-554 |
NN |
denotes |
plasma |
T3254 |
555-563 |
NN |
denotes |
membrane |
T3253 |
544-547 |
DT |
denotes |
the |
T3252 |
541-543 |
IN |
denotes |
on |
T3251 |
517-530 |
RB |
denotes |
predominantly |
T3250 |
514-516 |
VBZ |
denotes |
is |
T3249 |
508-513 |
NN |
denotes |
Acdp1 |
T3248 |
531-540 |
VBN |
denotes |
localized |
T3247 |
503-507 |
IN |
denotes |
that |
T3246 |
494-502 |
VBD |
denotes |
revealed |
T3245 |
486-493 |
NN |
denotes |
imaging |
T3244 |
477-485 |
JJ |
denotes |
Confocal |
T3243 |
476-564 |
sentence |
denotes |
Confocal imaging revealed that Acdp1 is predominantly localized on the plasma membrane. |
T3242 |
475-476 |
. |
denotes |
. |
T3241 |
458-466 |
JJ |
denotes |
specific |
T3240 |
448-449 |
HYPH |
denotes |
- |
T3239 |
449-457 |
NN |
denotes |
terminus |
T3238 |
447-448 |
NN |
denotes |
C |
T3237 |
441-446 |
NN |
denotes |
Acdp1 |
T3236 |
467-475 |
NN |
denotes |
antibody |
T3235 |
437-440 |
DT |
denotes |
the |
T3234 |
427-430 |
VBD |
denotes |
was |
T3233 |
431-436 |
VBG |
denotes |
using |
T3232 |
412-426 |
NN |
denotes |
Immunostaining |
T3231 |
411-476 |
sentence |
denotes |
Immunostaining was using the Acdp1 C-terminus specific antibody. |
T3230 |
410-411 |
. |
denotes |
. |
T3229 |
404-410 |
NNS |
denotes |
dishes |
T3228 |
401-403 |
IN |
denotes |
in |
T3227 |
381-389 |
JJ |
denotes |
cortical |
T3226 |
390-400 |
NNS |
denotes |
astrocytes |
T3225 |
375-380 |
NN |
denotes |
mouse |
T3224 |
372-374 |
IN |
denotes |
of |
T3223 |
352-361 |
JJ |
denotes |
confluent |
T3222 |
362-371 |
NN |
denotes |
monolayer |
T3221 |
350-351 |
DT |
denotes |
a |
T3220 |
345-349 |
IN |
denotes |
with |
T3219 |
338-344 |
VBN |
denotes |
coated |
T3218 |
327-337 |
NNS |
denotes |
coverslips |
T3217 |
321-326 |
NN |
denotes |
glass |
T3216 |
318-320 |
IN |
denotes |
on |
T3215 |
304-308 |
VBD |
denotes |
were |
T3214 |
309-317 |
VBN |
denotes |
cultured |
T3213 |
296-303 |
NNS |
denotes |
neurons |
T3212 |
292-295 |
DT |
denotes |
The |
T3211 |
291-411 |
sentence |
denotes |
The neurons were cultured on glass coverslips coated with a confluent monolayer of mouse cortical astrocytes in dishes. |
T3210 |
290-291 |
. |
denotes |
. |
T3209 |
283-290 |
NNS |
denotes |
embryos |
T3208 |
277-282 |
NN |
denotes |
mouse |
T3207 |
272-276 |
IN |
denotes |
from |
T3206 |
263-271 |
VBN |
denotes |
isolated |
T3205 |
255-262 |
NNS |
denotes |
neurons |
T3204 |
243-254 |
NN |
denotes |
hippocampus |
T3203 |
240-242 |
IN |
denotes |
in |
T3202 |
215-226 |
JJ |
denotes |
subcellular |
T3201 |
227-239 |
NN |
denotes |
localization |
T3200 |
211-214 |
PRP$ |
denotes |
its |
R1845 |
T3161 |
T3162 |
det |
The,specificity |
R1846 |
T3162 |
T3163 |
nsubj |
specificity,suggests |
R1847 |
T3164 |
T3162 |
prep |
of,specificity |
R1848 |
T3165 |
T3166 |
det |
the,antibody |
R1849 |
T3166 |
T3164 |
pobj |
antibody,of |
R1850 |
T3167 |
T3166 |
compound |
Acdp1,antibody |
R1851 |
T3168 |
T3169 |
compound |
C,terminus |
R1852 |
T3169 |
T3166 |
compound |
terminus,antibody |
R1853 |
T3170 |
T3169 |
punct |
-,terminus |
R1854 |
T3171 |
T3172 |
det |
the,possibility |
R1855 |
T3172 |
T3163 |
dobj |
possibility,suggests |
R1856 |
T3173 |
T3172 |
prep |
of,possibility |
R1857 |
T3174 |
T3173 |
pcomp |
using,of |
R1858 |
T3175 |
T3174 |
dobj |
it,using |
R1859 |
T3176 |
T3177 |
aux |
to,localize |
R1860 |
T3177 |
T3174 |
advcl |
localize,using |
R1861 |
T3178 |
T3177 |
dobj |
Acdp,localize |
R1862 |
T3179 |
T3178 |
punct |
-,Acdp |
R1863 |
T3180 |
T3178 |
nummod |
1,Acdp |
R1864 |
T3181 |
T3177 |
prep |
within,localize |
R1865 |
T3182 |
T3181 |
pobj |
cells,within |
R1866 |
T3183 |
T3163 |
punct |
.,suggests |
R1867 |
T3185 |
T3186 |
mark |
Since,revealed |
R1868 |
T3186 |
T3189 |
advcl |
revealed,examined |
R1869 |
T3187 |
T3188 |
compound |
Northern,blot |
R1870 |
T3188 |
T3186 |
nsubj |
blot,revealed |
R1871 |
T3190 |
T3191 |
advmod |
almost,exclusive |
R1872 |
T3191 |
T3192 |
amod |
exclusive,expression |
R1873 |
T3192 |
T3186 |
dobj |
expression,revealed |
R1874 |
T3193 |
T3192 |
prep |
of,expression |
R1875 |
T3194 |
T3193 |
pobj |
Acdp1,of |
R1876 |
T3195 |
T3186 |
prep |
in,revealed |
R1877 |
T3196 |
T3197 |
det |
the,brain |
R1878 |
T3197 |
T3195 |
pobj |
brain,in |
R1879 |
T3198 |
T3189 |
punct |
", ",examined |
R1880 |
T3199 |
T3189 |
nsubj |
we,examined |
R1881 |
T3200 |
T3201 |
poss |
its,localization |
R1882 |
T3201 |
T3189 |
dobj |
localization,examined |
R1883 |
T3202 |
T3201 |
amod |
subcellular,localization |
R1884 |
T3203 |
T3189 |
prep |
in,examined |
R1885 |
T3204 |
T3205 |
compound |
hippocampus,neurons |
R1886 |
T3205 |
T3203 |
pobj |
neurons,in |
R1887 |
T3206 |
T3205 |
acl |
isolated,neurons |
R1888 |
T3207 |
T3206 |
prep |
from,isolated |
R1889 |
T3208 |
T3209 |
compound |
mouse,embryos |
R1890 |
T3209 |
T3207 |
pobj |
embryos,from |
R1891 |
T3210 |
T3189 |
punct |
.,examined |
R1892 |
T3212 |
T3213 |
det |
The,neurons |
R1893 |
T3213 |
T3214 |
nsubjpass |
neurons,cultured |
R1894 |
T3215 |
T3214 |
auxpass |
were,cultured |
R1895 |
T3216 |
T3214 |
prep |
on,cultured |
R1896 |
T3217 |
T3218 |
compound |
glass,coverslips |
R1897 |
T3218 |
T3216 |
pobj |
coverslips,on |
R1898 |
T3219 |
T3218 |
acl |
coated,coverslips |
R1899 |
T3220 |
T3219 |
prep |
with,coated |
R1900 |
T3221 |
T3222 |
det |
a,monolayer |
R1901 |
T3222 |
T3220 |
pobj |
monolayer,with |
R1902 |
T3223 |
T3222 |
amod |
confluent,monolayer |
R1903 |
T3224 |
T3222 |
prep |
of,monolayer |
R1904 |
T3225 |
T3226 |
nmod |
mouse,astrocytes |
R1905 |
T3226 |
T3224 |
pobj |
astrocytes,of |
R1906 |
T3227 |
T3226 |
amod |
cortical,astrocytes |
R1907 |
T3228 |
T3214 |
prep |
in,cultured |
R1908 |
T3229 |
T3228 |
pobj |
dishes,in |
R1909 |
T3230 |
T3214 |
punct |
.,cultured |
R1910 |
T3232 |
T3233 |
nsubj |
Immunostaining,using |
R1911 |
T3234 |
T3233 |
aux |
was,using |
R1912 |
T3235 |
T3236 |
det |
the,antibody |
R1913 |
T3236 |
T3233 |
dobj |
antibody,using |
R1914 |
T3237 |
T3236 |
nmod |
Acdp1,antibody |
R1915 |
T3238 |
T3239 |
compound |
C,terminus |
R1916 |
T3239 |
T3241 |
npadvmod |
terminus,specific |
R1917 |
T3240 |
T3239 |
punct |
-,terminus |
R1918 |
T3241 |
T3236 |
amod |
specific,antibody |
R1919 |
T3242 |
T3233 |
punct |
.,using |
R1920 |
T3244 |
T3245 |
amod |
Confocal,imaging |
R1921 |
T3245 |
T3246 |
nsubj |
imaging,revealed |
R1922 |
T3247 |
T3248 |
mark |
that,localized |
R1923 |
T3248 |
T3246 |
ccomp |
localized,revealed |
R1924 |
T3249 |
T3248 |
nsubjpass |
Acdp1,localized |
R1925 |
T3250 |
T3248 |
auxpass |
is,localized |
R1926 |
T3251 |
T3248 |
advmod |
predominantly,localized |
R1927 |
T3252 |
T3248 |
prep |
on,localized |
R1928 |
T3253 |
T3254 |
det |
the,membrane |
R1929 |
T3254 |
T3252 |
pobj |
membrane,on |
R1930 |
T3255 |
T3254 |
compound |
plasma,membrane |
R1931 |
T3256 |
T3246 |
punct |
.,revealed |
R1932 |
T3258 |
T3259 |
det |
A,series |
R1933 |
T3259 |
T3260 |
nsubj |
series,showed |
R1934 |
T3261 |
T3259 |
prep |
of,series |
R1935 |
T3262 |
T3261 |
pobj |
sections,of |
R1936 |
T3263 |
T3262 |
prep |
of,sections |
R1937 |
T3264 |
T3265 |
det |
a,cell |
R1938 |
T3265 |
T3263 |
pobj |
cell,of |
R1939 |
T3266 |
T3259 |
prep |
at,series |
R1940 |
T3267 |
T3268 |
det |
the,thickness |
R1941 |
T3268 |
T3266 |
pobj |
thickness,at |
R1942 |
T3269 |
T3268 |
prep |
of,thickness |
R1943 |
T3270 |
T3271 |
nummod |
0.5,micrometer |
R1944 |
T3271 |
T3269 |
pobj |
micrometer,of |
R1945 |
T3272 |
T3260 |
advmod |
clearly,showed |
R1946 |
T3273 |
T3274 |
compound |
membrane,location |
R1947 |
T3274 |
T3260 |
dobj |
location,showed |
R1948 |
T3275 |
T3274 |
prep |
of,location |
R1949 |
T3276 |
T3277 |
compound |
Acdp1,immunoreactivity |
R1950 |
T3277 |
T3275 |
pobj |
immunoreactivity,of |
R1951 |
T3278 |
T3277 |
punct |
-,immunoreactivity |
R1952 |
T3279 |
T3280 |
punct |
(,Fig. |
R1953 |
T3280 |
T3277 |
parataxis |
Fig.,immunoreactivity |
R1954 |
T3281 |
T3280 |
nummod |
7,Fig. |
R1955 |
T3282 |
T3280 |
punct |
),Fig. |
R1956 |
T3283 |
T3260 |
punct |
", ",showed |
R1957 |
T3284 |
T3285 |
dep |
which,is |
R1958 |
T3285 |
T3260 |
advcl |
is,showed |
R1959 |
T3286 |
T3285 |
acomp |
consistent,is |
R1960 |
T3287 |
T3286 |
prep |
with,consistent |
R1961 |
T3288 |
T3289 |
det |
the,observation |
R1962 |
T3289 |
T3287 |
pobj |
observation,with |
R1963 |
T3290 |
T3289 |
prep |
of,observation |
R1964 |
T3291 |
T3292 |
compound |
transmembrane,domains |
R1965 |
T3292 |
T3290 |
pobj |
domains,of |
R1966 |
T3293 |
T3289 |
prep |
within,observation |
R1967 |
T3294 |
T3295 |
det |
the,proteins |
R1968 |
T3295 |
T3293 |
pobj |
proteins,within |
R1969 |
T3296 |
T3295 |
compound |
Acdp,proteins |
R1970 |
T3297 |
T3260 |
punct |
.,showed |