PMC:3359311 / 8680-9425 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T13245","span":{"begin":721,"end":725},"obj":"P05231"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T13115","span":{"begin":721,"end":725},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T13403","span":{"begin":84,"end":93},"obj":"Protein"},{"id":"T13404","span":{"begin":73,"end":83},"obj":"Positive_regulation"},{"id":"T13405","span":{"begin":721,"end":725},"obj":"Protein"}],"relations":[{"id":"R11887","pred":"themeOf","subj":"T13403","obj":"T13404"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"events-check-again","denotations":[{"id":"T13402","span":{"begin":721,"end":725},"obj":"Protein"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"GO-CC","denotations":[{"id":"T13394","span":{"begin":51,"end":56},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13395","span":{"begin":153,"end":158},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13396","span":{"begin":386,"end":391},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13397","span":{"begin":489,"end":494},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13398","span":{"begin":502,"end":507},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T13399","span":{"begin":650,"end":654},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"GO-MF","denotations":[{"id":"T13393","span":{"begin":721,"end":725},"obj":"http://purl.obolibrary.org/obo/GO_0005138"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

{"project":"GO-BP","denotations":[{"id":"T13392","span":{"begin":94,"end":114},"obj":"http://purl.obolibrary.org/obo/GO_0014823"}],"text":"10.1371/journal.pone.0037689.g003 Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

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Figure 3 Airway cells do not strongly upregulate caspase-1 activity in response to inflammasome stimuli.\nS9 and IB3-1 cells were examined for caspase-1 activation following inflammasome stimulation with P. aeruginosa (MOI = 10) and ATP (5 mM). Cells were primed with LPS for 5 hours where appropriate. A representative histogram of % caspase-1-active cells is shown in (A) and the averaged values are shown in (B) (n = 3 separate experiments). (C) IB3-1 cells (5×104 cells/well) were pre-treated for 1 hour with increasing concentrations of z-YVAD-fmk (10–30 µM) prior to stimulation with P. aeruginosa (MOI = 50). Cell culture supernatants were collected after 6 hours and assayed for IL-6 by ELISA (n = 3).\n\n"}

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