PMC:3359311 / 5757-7939 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3359311","sourcedb":"PMC","sourceid":"3359311","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3359311","text":"Airway epithelial cells do not produce significant amounts of IL-1β in response to inflammasome stimulation\nThe inflammasomes and their respective activators examined in this study are listed in Table 1. Cells were stimulated in accordance with the schedule in Figure 1. In CF, airway epithelial cells have been shown to possess a hyper-inflammatory phenotype and produce an exaggerated pro-inflammatory cytokine response [4], [5]. To determine if airway epithelial cells contribute to the increased IL-1β production in patients with CF, CF and control bronchial epithelial cell lines were stimulated with the inflammasome inducers P. aeruginosa strain PAO1 (PAO1) and LPS followed by ATP. IL-1β levels in cell culture supernatants were not greatly increased in either the CF or control cell lines (Fig. 2a–d), although a small increase in IL-1β production was detected in NuLi-1 and CuFi-1 cells, but not in S9 and IB3-1 cells, by 24 hours. In contrast, these airway cells were highly responsive to other inflammatory stimuli, such as recombinant IL-1β, producing large quantities of IL-8 (Fig. 2a–d inserts).\n10.1371/journal.pone.0037689.g001 Figure 1 Cell stimulation and inhibitor schedule.\nSchedule outlines the timing of inhibitor addition and priming in relation to inflammasome stimulation (t = 0) for THP-1 reporter and PBMC cytokine quantification experiments. Inhibitor treatments and stimulations were carried out as described in the Materials and Methods section.\n10.1371/journal.pone.0037689.g002 Figure 2 Airway epithelial cells do not significantly contribute to IL-1β production in response to inflammasome stimuli.\nControl cell lines ((A) S9, (C) NuLi-1) and their corresponding CF cell lines ((B) IB3-1, and (D) CuFi-1) cells were stimulated with P. aeruginosa (MOI = 10), ATP (5 mM), or IL-1β (10 ng/ml), for the indicated times (n = 3 individual experiments). Cells were primed with LPS (100 ng/ml) for 4 hours where appropriate. Cell culture supernatants were assayed for IL-1β and IL-8 production by ELISA. Insert shows IL-8 secretion in response to stimulation with IL-1β (10 ng/ml).\n10.1371/journal.pone.0037689.t001 Table 1 Inflammasome Activators.\n\nAir","divisions":[{"label":"Title","span":{"begin":0,"end":107}},{"label":"Figure caption","span":{"begin":1111,"end":1479}},{"label":"Title","span":{"begin":1155,"end":1195}},{"label":"Figure caption","span":{"begin":1478,"end":2111}},{"label":"Title","span":{"begin":1522,"end":1634}},{"label":"Table 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