PMC:3359311 / 33168-33966
Annnotations
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T12602","span":{"begin":419,"end":423},"obj":"Protein"},{"id":"T12603","span":{"begin":530,"end":534},"obj":"Protein"},{"id":"T12604","span":{"begin":708,"end":712},"obj":"Protein"},{"id":"T12605","span":{"begin":791,"end":795},"obj":"Protein"}],"text":"Bacterial strains\nP. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase. Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm). To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour. For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection. Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.\n\n"}
bionlp-st-ge-2016-spacy-parsed
{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T12443","span":{"begin":0,"end":9},"obj":"JJ"},{"id":"T12444","span":{"begin":10,"end":17},"obj":"NNS"},{"id":"T12445","span":{"begin":18,"end":20},"obj":"NNP"},{"id":"T12446","span":{"begin":21,"end":31},"obj":"NN"},{"id":"T12447","span":{"begin":32,"end":42},"obj":"NN"},{"id":"T12448","span":{"begin":43,"end":50},"obj":"NNS"},{"id":"T12449","span":{"begin":51,"end":55},"obj":"NNP"},{"id":"T12450","span":{"begin":56,"end":59},"obj":"CC"},{"id":"T12451","span":{"begin":60,"end":63},"obj":"DT"},{"id":"T12452","span":{"begin":64,"end":73},"obj":"NNP"},{"id":"T12453","span":{"begin":74,"end":80},"obj":"JJ"},{"id":"T12454","span":{"begin":81,"end":85},"obj":"VBD"},{"id":"T12455","span":{"begin":86,"end":94},"obj":"VBN"},{"id":"T12456","span":{"begin":95,"end":99},"obj":"IN"},{"id":"T12457","span":{"begin":100,"end":103},"obj":"NNP"},{"id":"T12458","span":{"begin":104,"end":110},"obj":"NNP"},{"id":"T12459","span":{"begin":111,"end":118},"obj":"NNP"},{"id":"T12460","span":{"begin":118,"end":119},"obj":"."},{"id":"T12461","span":{"begin":120,"end":122},"obj":"NNP"},{"id":"T12462","span":{"begin":123,"end":133},"obj":"NN"},{"id":"T12463","span":{"begin":134,"end":141},"obj":"NNS"},{"id":"T12464","span":{"begin":142,"end":146},"obj":"NNP"},{"id":"T12465","span":{"begin":147,"end":150},"obj":"CC"},{"id":"T12466","span":{"begin":151,"end":160},"obj":"NNP"},{"id":"T12467","span":{"begin":161,"end":165},"obj":"VBD"},{"id":"T12468","span":{"begin":166,"end":171},"obj":"VBN"},{"id":"T12469","span":{"begin":172,"end":176},"obj":"IN"},{"id":"T12470","span":{"begin":177,"end":186},"obj":"JJ"},{"id":"T12471","span":{"begin":187,"end":195},"obj":"NNS"},{"id":"T12472","span":{"begin":196,"end":198},"obj":"IN"},{"id":"T12473","span":{"begin":199,"end":204},"obj":"NNP"},{"id":"T12474","span":{"begin":205,"end":212},"obj":"NNP"},{"id":"T12475","span":{"begin":213,"end":214},"obj":"-LRB-"},{"id":"T12476","span":{"begin":214,"end":216},"obj":"NNP"},{"id":"T12477","span":{"begin":216,"end":217},"obj":"-RRB-"},{"id":"T12478","span":{"begin":218,"end":223},"obj":"NN"},{"id":"T12479","span":{"begin":224,"end":227},"obj":"CC"},{"id":"T12480","span":{"begin":228,"end":230},"obj":"NNP"},{"id":"T12481","span":{"begin":230,"end":231},"obj":"NNP"},{"id":"T12482","span":{"begin":231,"end":243},"obj":"NNP"},{"id":"T12483","span":{"begin":244,"end":245},"obj":"-LRB-"},{"id":"T12484","span":{"begin":245,"end":248},"obj":"CD"},{"id":"T12485","span":{"begin":249,"end":251},"obj":"NN"},{"id":"T12486","span":{"begin":251,"end":252},"obj":"NN"},{"id":"T12487","span":{"begin":252,"end":254},"obj":"NN"},{"id":"T12488","span":{"begin":254,"end":255},"obj":"-RRB-"},{"id":"T12489","span":{"begin":256,"end":261},"obj":"IN"},{"id":"T12490","span":{"begin":262,"end":277},"obj":"JJ"},{"id":"T12491","span":{"begin":278,"end":283},"obj":"NN"},{"id":"T12492","span":{"begin":283,"end":284},"obj":"."},{"id":"T12493","span":{"begin":285,"end":290},"obj":"NNS"},{"id":"T12494","span":{"begin":291,"end":295},"obj":"VBD"},{"id":"T12495","span":{"begin":296,"end":302},"obj":"VBN"},{"id":"T12496","span":{"begin":303,"end":307},"obj":"RB"},{"id":"T12497","span":{"begin":308,"end":310},"obj":"IN"},{"id":"T12498","span":{"begin":311,"end":314},"obj":"NNP"},{"id":"T12499","span":{"begin":315,"end":318},"obj":"CC"},{"id":"T12500","span":{"begin":319,"end":330},"obj":"VBD"},{"id":"T12501","span":{"begin":331,"end":333},"obj":"IN"},{"id":"T12502","span":{"begin":334,"end":337},"obj":"NNP"},{"id":"T12503","span":{"begin":338,"end":340},"obj":"TO"},{"id":"T12504","span":{"begin":341,"end":343},"obj":"DT"},{"id":"T12505","span":{"begin":344,"end":351},"obj":"JJ"},{"id":"T12506","span":{"begin":352,"end":359},"obj":"NN"},{"id":"T12507","span":{"begin":360,"end":362},"obj":"IN"},{"id":"T12508","span":{"begin":363,"end":366},"obj":"CD"},{"id":"T12509","span":{"begin":367,"end":368},"obj":"-LRB-"},{"id":"T12510","span":{"begin":368,"end":369},"obj":"NN"},{"id":"T12511","span":{"begin":370,"end":371},"obj":"SYM"},{"id":"T12512","span":{"begin":372,"end":375},"obj":"CD"},{"id":"T12513","span":{"begin":376,"end":378},"obj":"NN"},{"id":"T12514","span":{"begin":378,"end":379},"obj":"-RRB-"},{"id":"T12515","span":{"begin":379,"end":380},"obj":"."},{"id":"T12516","span":{"begin":381,"end":383},"obj":"TO"},{"id":"T12517","span":{"begin":384,"end":391},"obj":"VB"},{"id":"T12518","span":{"begin":392,"end":403},"obj":"JJ"},{"id":"T12519","span":{"begin":404,"end":412},"obj":"NNS"},{"id":"T12520","span":{"begin":412,"end":413},"obj":","},{"id":"T12521","span":{"begin":414,"end":418},"obj":"VBP"},{"id":"T12522","span":{"begin":419,"end":423},"obj":"CD"},{"id":"T12523","span":{"begin":424,"end":427},"obj":"VBD"},{"id":"T12524","span":{"begin":428,"end":439},"obj":"VBN"},{"id":"T12525","span":{"begin":440,"end":442},"obj":"IN"},{"id":"T12526","span":{"begin":443,"end":446},"obj":"NNP"},{"id":"T12527","span":{"begin":447,"end":449},"obj":"TO"},{"id":"T12528","span":{"begin":450,"end":452},"obj":"DT"},{"id":"T12529","span":{"begin":453,"end":460},"obj":"JJ"},{"id":"T12530","span":{"begin":461,"end":468},"obj":"NN"},{"id":"T12531","span":{"begin":469,"end":471},"obj":"IN"},{"id":"T12532","span":{"begin":472,"end":475},"obj":"CD"},{"id":"T12533","span":{"begin":476,"end":479},"obj":"CC"},{"id":"T12534","span":{"begin":480,"end":486},"obj":"VBN"},{"id":"T12535","span":{"begin":487,"end":489},"obj":"IN"},{"id":"T12536","span":{"begin":490,"end":492},"obj":"CD"},{"id":"T12537","span":{"begin":492,"end":493},"obj":"CD"},{"id":"T12538","span":{"begin":493,"end":494},"obj":"NNP"},{"id":"T12539","span":{"begin":495,"end":498},"obj":"IN"},{"id":"T12540","span":{"begin":499,"end":500},"obj":"CD"},{"id":"T12541","span":{"begin":501,"end":505},"obj":"NN"},{"id":"T12542","span":{"begin":505,"end":506},"obj":"."},{"id":"T12543","span":{"begin":507,"end":510},"obj":"IN"},{"id":"T12544","span":{"begin":511,"end":523},"obj":"NNS"},{"id":"T12545","span":{"begin":523,"end":524},"obj":","},{"id":"T12546","span":{"begin":525,"end":529},"obj":"VBP"},{"id":"T12547","span":{"begin":530,"end":534},"obj":"CD"},{"id":"T12548","span":{"begin":535,"end":538},"obj":"VBD"},{"id":"T12549","span":{"begin":539,"end":550},"obj":"VBN"},{"id":"T12550","span":{"begin":551,"end":553},"obj":"TO"},{"id":"T12551","span":{"begin":554,"end":556},"obj":"DT"},{"id":"T12552","span":{"begin":557,"end":564},"obj":"JJ"},{"id":"T12553","span":{"begin":565,"end":572},"obj":"NN"},{"id":"T12554","span":{"begin":573,"end":575},"obj":"IN"},{"id":"T12555","span":{"begin":576,"end":579},"obj":"CD"},{"id":"T12556","span":{"begin":580,"end":582},"obj":"IN"},{"id":"T12557","span":{"begin":583,"end":586},"obj":"NNP"},{"id":"T12558","span":{"begin":587,"end":590},"obj":"CC"},{"id":"T12559","span":{"begin":591,"end":598},"obj":"JJ"},{"id":"T12560","span":{"begin":599,"end":606},"obj":"VBN"},{"id":"T12561","span":{"begin":607,"end":609},"obj":"IN"},{"id":"T12562","span":{"begin":610,"end":617},"obj":"NN"},{"id":"T12563","span":{"begin":618,"end":624},"obj":"NN"},{"id":"T12564","span":{"begin":625,"end":630},"obj":"RB"},{"id":"T12565","span":{"begin":631,"end":633},"obj":"TO"},{"id":"T12566","span":{"begin":634,"end":645},"obj":"NN"},{"id":"T12567","span":{"begin":646,"end":648},"obj":"TO"},{"id":"T12568","span":{"begin":649,"end":656},"obj":"VB"},{"id":"T12569","span":{"begin":657,"end":660},"obj":"DT"},{"id":"T12570","span":{"begin":661,"end":668},"obj":"VBN"},{"id":"T12571","span":{"begin":669,"end":681},"obj":"NN"},{"id":"T12572","span":{"begin":682,"end":684},"obj":"IN"},{"id":"T12573","span":{"begin":685,"end":694},"obj":"NN"},{"id":"T12574","span":{"begin":694,"end":695},"obj":"."},{"id":"T12575","span":{"begin":696,"end":707},"obj":"JJ"},{"id":"T12576","span":{"begin":708,"end":712},"obj":"NNP"},{"id":"T12577","span":{"begin":713,"end":716},"obj":"VBD"},{"id":"T12578","span":{"begin":717,"end":722},"obj":"VBN"},{"id":"T12579","span":{"begin":723,"end":725},"obj":"IN"},{"id":"T12580","span":{"begin":726,"end":727},"obj":"DT"},{"id":"T12581","span":{"begin":728,"end":734},"obj":"NN"},{"id":"T12582","span":{"begin":735,"end":745},"obj":"NN"},{"id":"T12583","span":{"begin":746,"end":748},"obj":"TO"},{"id":"T12584","span":{"begin":749,"end":753},"obj":"DT"},{"id":"T12585","span":{"begin":754,"end":758},"obj":"VBN"},{"id":"T12586","span":{"begin":759,"end":761},"obj":"TO"},{"id":"T12587","span":{"begin":762,"end":769},"obj":"VB"},{"id":"T12588","span":{"begin":770,"end":772},"obj":"DT"},{"id":"T12589","span":{"begin":773,"end":776},"obj":"NNP"},{"id":"T12590","span":{"begin":777,"end":779},"obj":"IN"},{"id":"T12591","span":{"begin":780,"end":781},"obj":"CD"},{"id":"T12592","span":{"begin":782,"end":785},"obj":"IN"},{"id":"T12593","span":{"begin":786,"end":790},"obj":"JJ"},{"id":"T12594","span":{"begin":791,"end":795},"obj":"NN"},{"id":"T12595","span":{"begin":795,"end":796},"obj":"."}],"relations":[{"id":"R11027","pred":"amod","subj":"T12443","obj":"T12444"},{"id":"R11028","pred":"nsubjpass","subj":"T12444","obj":"T12455"},{"id":"R11029","pred":"npadvmod","subj":"T12445","obj":"T12455"},{"id":"R11030","pred":"nmod","subj":"T12446","obj":"T12448"},{"id":"R11031","pred":"compound","subj":"T12447","obj":"T12448"},{"id":"R11032","pred":"nsubjpass","subj":"T12448","obj":"T12455"},{"id":"R11033","pred":"appos","subj":"T12449","obj":"T12448"},{"id":"R11034","pred":"cc","subj":"T12450","obj":"T12449"},{"id":"R11035","pred":"det","subj":"T12451","obj":"T12453"},{"id":"R11036","pred":"compound","subj":"T12452","obj":"T12453"},{"id":"R11037","pred":"conj","subj":"T12453","obj":"T12449"},{"id":"R11038","pred":"auxpass","subj":"T12454","obj":"T12455"},{"id":"R11039","pred":"ROOT","subj":"T12455","obj":"T12455"},{"id":"R11040","pred":"prep","subj":"T12456","obj":"T12455"},{"id":"R11041","pred":"compound","subj":"T12457","obj":"T12459"},{"id":"R11042","pred":"compound","subj":"T12458","obj":"T12459"},{"id":"R11043","pred":"pobj","subj":"T12459","obj":"T12456"},{"id":"R11044","pred":"punct","subj":"T12460","obj":"T12455"},{"id":"R11045","pred":"nsubjpass","subj":"T12461","obj":"T12468"},{"id":"R11046","pred":"compound","subj":"T12462","obj":"T12463"},{"id":"R11047","pred":"nsubjpass","subj":"T12463","obj":"T12468"},{"id":"R11048","pred":"appos","subj":"T12464","obj":"T12463"},{"id":"R11049","pred":"cc","subj":"T12465","obj":"T12464"},{"id":"R11050","pred":"conj","subj":"T12466","obj":"T12464"},{"id":"R11051","pred":"auxpass","subj":"T12467","obj":"T12468"},{"id":"R11052","pred":"ROOT","subj":"T12468","obj":"T12468"},{"id":"R11053","pred":"prep","subj":"T12469","obj":"T12468"},{"id":"R11054","pred":"amod","subj":"T12470","obj":"T12471"},{"id":"R11055","pred":"pobj","subj":"T12471","obj":"T12469"},{"id":"R11056","pred":"prep","subj":"T12472","obj":"T12471"},{"id":"R11057","pred":"compound","subj":"T12473","obj":"T12474"},{"id":"R11058","pred":"pobj","subj":"T12474","obj":"T12472"},{"id":"R11059","pred":"punct","subj":"T12475","obj":"T12474"},{"id":"R11060","pred":"appos","subj":"T12476","obj":"T12474"},{"id":"R11061","pred":"punct","subj":"T12477","obj":"T12474"},{"id":"R11062","pred":"conj","subj":"T12478","obj":"T12471"},{"id":"R11063","pred":"cc","subj":"T12479","obj":"T12478"},{"id":"R11064","pred":"compound","subj":"T12480","obj":"T12482"},{"id":"R11065","pred":"compound","subj":"T12481","obj":"T12482"},{"id":"R11066","pred":"appos","subj":"T12482","obj":"T12471"},{"id":"R11067","pred":"punct","subj":"T12483","obj":"T12487"},{"id":"R11068","pred":"nummod","subj":"T12484","obj":"T12485"},{"id":"R11069","pred":"nmod","subj":"T12485","obj":"T12487"},{"id":"R11070","pred":"compound","subj":"T12486","obj":"T12487"},{"id":"R11071","pred":"appos","subj":"T12487","obj":"T12482"},{"id":"R11072","pred":"punct","subj":"T12488","obj":"T12487"},{"id":"R11073","pred":"prep","subj":"T12489","obj":"T12469"},{"id":"R11074","pred":"amod","subj":"T12490","obj":"T12491"},{"id":"R11075","pred":"pobj","subj":"T12491","obj":"T12489"},{"id":"R11076","pred":"punct","subj":"T12492","obj":"T12468"},{"id":"R11077","pred":"nsubjpass","subj":"T12493","obj":"T12495"},{"id":"R11078","pred":"auxpass","subj":"T12494","obj":"T12495"},{"id":"R11079","pred":"ROOT","subj":"T12495","obj":"T12495"},{"id":"R11080","pred":"advmod","subj":"T12496","obj":"T12495"},{"id":"R11081","pred":"prep","subj":"T12497","obj":"T12495"},{"id":"R11082","pred":"pobj","subj":"T12498","obj":"T12497"},{"id":"R11083","pred":"cc","subj":"T12499","obj":"T12495"},{"id":"R11084","pred":"conj","subj":"T12500","obj":"T12495"},{"id":"R11085","pred":"prep","subj":"T12501","obj":"T12500"},{"id":"R11086","pred":"pobj","subj":"T12502","obj":"T12501"},{"id":"R11087","pred":"prep","subj":"T12503","obj":"T12500"},{"id":"R11088","pred":"det","subj":"T12504","obj":"T12506"},{"id":"R11089","pred":"amod","subj":"T12505","obj":"T12506"},{"id":"R11090","pred":"pobj","subj":"T12506","obj":"T12503"},{"id":"R11091","pred":"prep","subj":"T12507","obj":"T12506"},{"id":"R11092","pred":"pobj","subj":"T12508","obj":"T12507"},{"id":"R11093","pred":"punct","subj":"T12509","obj":"T12513"},{"id":"R11094","pred":"dep","subj":"T12510","obj":"T12512"},{"id":"R11095","pred":"punct","subj":"T12511","obj":"T12512"},{"id":"R11096","pred":"nummod","subj":"T12512","obj":"T12513"},{"id":"R11097","pred":"appos","subj":"T12513","obj":"T12506"},{"id":"R11098","pred":"punct","subj":"T12514","obj":"T12500"},{"id":"R11099","pred":"punct","subj":"T12515","obj":"T12495"},{"id":"R11100","pred":"aux","subj":"T12516","obj":"T12517"},{"id":"R11101","pred":"advcl","subj":"T12517","obj":"T12521"},{"id":"R11102","pred":"amod","subj":"T12518","obj":"T12519"},{"id":"R11103","pred":"dobj","subj":"T12519","obj":"T12517"},{"id":"R11104","pred":"punct","subj":"T12520","obj":"T12521"},{"id":"R11105","pred":"ROOT","subj":"T12521","obj":"T12521"},{"id":"R11106","pred":"nsubjpass","subj":"T12522","obj":"T12524"},{"id":"R11107","pred":"auxpass","subj":"T12523","obj":"T12524"},{"id":"R11108","pred":"ccomp","subj":"T12524","obj":"T12521"},{"id":"R11109","pred":"prep","subj":"T12525","obj":"T12524"},{"id":"R11110","pred":"pobj","subj":"T12526","obj":"T12525"},{"id":"R11111","pred":"prep","subj":"T12527","obj":"T12524"},{"id":"R11112","pred":"det","subj":"T12528","obj":"T12530"},{"id":"R11113","pred":"amod","subj":"T12529","obj":"T12530"},{"id":"R11114","pred":"pobj","subj":"T12530","obj":"T12527"},{"id":"R11115","pred":"prep","subj":"T12531","obj":"T12530"},{"id":"R11116","pred":"pobj","subj":"T12532","obj":"T12531"},{"id":"R11117","pred":"cc","subj":"T12533","obj":"T12524"},{"id":"R11118","pred":"conj","subj":"T12534","obj":"T12524"},{"id":"R11119","pred":"prep","subj":"T12535","obj":"T12534"},{"id":"R11120","pred":"nummod","subj":"T12536","obj":"T12537"},{"id":"R11121","pred":"pobj","subj":"T12537","obj":"T12535"},{"id":"R11122","pred":"conj","subj":"T12538","obj":"T12524"},{"id":"R11123","pred":"prep","subj":"T12539","obj":"T12538"},{"id":"R11124","pred":"nummod","subj":"T12540","obj":"T12541"},{"id":"R11125","pred":"pobj","subj":"T12541","obj":"T12539"},{"id":"R11126","pred":"punct","subj":"T12542","obj":"T12521"},{"id":"R11127","pred":"prep","subj":"T12543","obj":"T12546"},{"id":"R11128","pred":"pobj","subj":"T12544","obj":"T12543"},{"id":"R11129","pred":"punct","subj":"T12545","obj":"T12546"},{"id":"R11130","pred":"ROOT","subj":"T12546","obj":"T12546"},{"id":"R11131","pred":"nsubjpass","subj":"T12547","obj":"T12549"},{"id":"R11132","pred":"auxpass","subj":"T12548","obj":"T12549"},{"id":"R11133","pred":"ccomp","subj":"T12549","obj":"T12546"},{"id":"R11134","pred":"prep","subj":"T12550","obj":"T12549"},{"id":"R11135","pred":"det","subj":"T12551","obj":"T12553"},{"id":"R11136","pred":"amod","subj":"T12552","obj":"T12553"},{"id":"R11137","pred":"pobj","subj":"T12553","obj":"T12550"},{"id":"R11138","pred":"prep","subj":"T12554","obj":"T12553"},{"id":"R11139","pred":"pobj","subj":"T12555","obj":"T12554"},{"id":"R11140","pred":"prep","subj":"T12556","obj":"T12553"},{"id":"R11141","pred":"pobj","subj":"T12557","obj":"T12556"},{"id":"R11142","pred":"cc","subj":"T12558","obj":"T12549"},{"id":"R11143","pred":"advmod","subj":"T12559","obj":"T12560"},{"id":"R11144","pred":"conj","subj":"T12560","obj":"T12549"},{"id":"R11145","pred":"prep","subj":"T12561","obj":"T12560"},{"id":"R11146","pred":"compound","subj":"T12562","obj":"T12563"},{"id":"R11147","pred":"pobj","subj":"T12563","obj":"T12561"},{"id":"R11148","pred":"advmod","subj":"T12564","obj":"T12560"},{"id":"R11149","pred":"prep","subj":"T12565","obj":"T12564"},{"id":"R11150","pred":"pobj","subj":"T12566","obj":"T12565"},{"id":"R11151","pred":"aux","subj":"T12567","obj":"T12568"},{"id":"R11152","pred":"advcl","subj":"T12568","obj":"T12560"},{"id":"R11153","pred":"det","subj":"T12569","obj":"T12571"},{"id":"R11154","pred":"amod","subj":"T12570","obj":"T12571"},{"id":"R11155","pred":"dobj","subj":"T12571","obj":"T12568"},{"id":"R11156","pred":"prep","subj":"T12572","obj":"T12571"},{"id":"R11157","pred":"pobj","subj":"T12573","obj":"T12572"},{"id":"R11158","pred":"punct","subj":"T12574","obj":"T12546"},{"id":"R11159","pred":"compound","subj":"T12575","obj":"T12576"},{"id":"R11160","pred":"nsubjpass","subj":"T12576","obj":"T12578"},{"id":"R11161","pred":"auxpass","subj":"T12577","obj":"T12578"},{"id":"R11162","pred":"ROOT","subj":"T12578","obj":"T12578"},{"id":"R11163","pred":"prep","subj":"T12579","obj":"T12578"},{"id":"R11164","pred":"det","subj":"T12580","obj":"T12582"},{"id":"R11165","pred":"compound","subj":"T12581","obj":"T12582"},{"id":"R11166","pred":"pobj","subj":"T12582","obj":"T12579"},{"id":"R11167","pred":"prep","subj":"T12583","obj":"T12582"},{"id":"R11168","pred":"det","subj":"T12584","obj":"T12585"},{"id":"R11169","pred":"pobj","subj":"T12585","obj":"T12583"},{"id":"R11170","pred":"aux","subj":"T12586","obj":"T12587"},{"id":"R11171","pred":"xcomp","subj":"T12587","obj":"T12585"},{"id":"R11172","pred":"det","subj":"T12588","obj":"T12589"},{"id":"R11173","pred":"dobj","subj":"T12589","obj":"T12587"},{"id":"R11174","pred":"prep","subj":"T12590","obj":"T12589"},{"id":"R11175","pred":"pobj","subj":"T12591","obj":"T12590"},{"id":"R11176","pred":"prep","subj":"T12592","obj":"T12587"},{"id":"R11177","pred":"amod","subj":"T12593","obj":"T12594"},{"id":"R11178","pred":"pobj","subj":"T12594","obj":"T12592"},{"id":"R11179","pred":"punct","subj":"T12595","obj":"T12578"}],"text":"Bacterial strains\nP. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase. Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm). To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour. For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection. Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.\n\n"}
pmc-enju-pas
{"project":"pmc-enju-pas","denotations":[{"id":"T12383","span":{"begin":469,"end":471},"obj":"IN"},{"id":"T12304","span":{"begin":0,"end":9},"obj":"JJ"},{"id":"T12305","span":{"begin":10,"end":17},"obj":"NN"},{"id":"T12306","span":{"begin":18,"end":20},"obj":"NNP"},{"id":"T12307","span":{"begin":21,"end":31},"obj":"NN"},{"id":"T12308","span":{"begin":32,"end":42},"obj":"NN"},{"id":"T12309","span":{"begin":43,"end":50},"obj":"NN"},{"id":"T12310","span":{"begin":51,"end":55},"obj":"NN"},{"id":"T12311","span":{"begin":56,"end":59},"obj":"CC"},{"id":"T12312","span":{"begin":60,"end":63},"obj":"DT"},{"id":"T12313","span":{"begin":64,"end":73},"obj":"NN"},{"id":"T12314","span":{"begin":74,"end":80},"obj":"NN"},{"id":"T12315","span":{"begin":81,"end":85},"obj":"VB"},{"id":"T12316","span":{"begin":86,"end":94},"obj":"VB"},{"id":"T12317","span":{"begin":95,"end":99},"obj":"IN"},{"id":"T12318","span":{"begin":100,"end":103},"obj":"NNP"},{"id":"T12319","span":{"begin":104,"end":110},"obj":"NNP"},{"id":"T12320","span":{"begin":111,"end":119},"obj":"NNP"},{"id":"T12321","span":{"begin":120,"end":122},"obj":"NNP"},{"id":"T12322","span":{"begin":123,"end":133},"obj":"NN"},{"id":"T12323","span":{"begin":134,"end":141},"obj":"NN"},{"id":"T12324","span":{"begin":142,"end":146},"obj":"NN"},{"id":"T12325","span":{"begin":147,"end":150},"obj":"CC"},{"id":"T12326","span":{"begin":151,"end":160},"obj":"NN"},{"id":"T12327","span":{"begin":161,"end":165},"obj":"VB"},{"id":"T12328","span":{"begin":166,"end":171},"obj":"VB"},{"id":"T12329","span":{"begin":172,"end":176},"obj":"IN"},{"id":"T12330","span":{"begin":177,"end":186},"obj":"JJ"},{"id":"T12331","span":{"begin":187,"end":195},"obj":"NN"},{"id":"T12332","span":{"begin":196,"end":198},"obj":"IN"},{"id":"T12333","span":{"begin":199,"end":204},"obj":"NNP"},{"id":"T12334","span":{"begin":205,"end":212},"obj":"NNP"},{"id":"T12335","span":{"begin":213,"end":214},"obj":"-LRB-"},{"id":"T12336","span":{"begin":214,"end":216},"obj":"NN"},{"id":"T12337","span":{"begin":216,"end":217},"obj":"-RRB-"},{"id":"T12338","span":{"begin":218,"end":223},"obj":"NN"},{"id":"T12339","span":{"begin":224,"end":227},"obj":"CC"},{"id":"T12340","span":{"begin":228,"end":243},"obj":"NN"},{"id":"T12341","span":{"begin":244,"end":245},"obj":"-LRB-"},{"id":"T12342","span":{"begin":245,"end":248},"obj":"CD"},{"id":"T12343","span":{"begin":249,"end":254},"obj":"NN"},{"id":"T12344","span":{"begin":254,"end":255},"obj":"-RRB-"},{"id":"T12345","span":{"begin":256,"end":261},"obj":"IN"},{"id":"T12346","span":{"begin":262,"end":277},"obj":"JJ"},{"id":"T12347","span":{"begin":278,"end":283},"obj":"NN"},{"id":"T12348","span":{"begin":285,"end":290},"obj":"NN"},{"id":"T12349","span":{"begin":291,"end":295},"obj":"VB"},{"id":"T12350","span":{"begin":296,"end":302},"obj":"VB"},{"id":"T12351","span":{"begin":303,"end":307},"obj":"RB"},{"id":"T12352","span":{"begin":308,"end":310},"obj":"IN"},{"id":"T12353","span":{"begin":311,"end":314},"obj":"NNP"},{"id":"T12354","span":{"begin":315,"end":318},"obj":"CC"},{"id":"T12355","span":{"begin":319,"end":330},"obj":"VB"},{"id":"T12356","span":{"begin":331,"end":333},"obj":"IN"},{"id":"T12357","span":{"begin":334,"end":337},"obj":"NNP"},{"id":"T12358","span":{"begin":338,"end":340},"obj":"TO"},{"id":"T12359","span":{"begin":341,"end":343},"obj":"DT"},{"id":"T12360","span":{"begin":344,"end":351},"obj":"JJ"},{"id":"T12361","span":{"begin":352,"end":359},"obj":"NN"},{"id":"T12362","span":{"begin":360,"end":362},"obj":"IN"},{"id":"T12363","span":{"begin":363,"end":366},"obj":"CD"},{"id":"T12364","span":{"begin":367,"end":368},"obj":"-LRB-"},{"id":"T12365","span":{"begin":368,"end":375},"obj":"CD"},{"id":"T12366","span":{"begin":376,"end":378},"obj":"NN"},{"id":"T12367","span":{"begin":378,"end":379},"obj":"-RRB-"},{"id":"T12368","span":{"begin":381,"end":383},"obj":"TO"},{"id":"T12369","span":{"begin":384,"end":391},"obj":"VB"},{"id":"T12370","span":{"begin":392,"end":403},"obj":"JJ"},{"id":"T12371","span":{"begin":404,"end":412},"obj":"NN"},{"id":"T12372","span":{"begin":412,"end":413},"obj":"-COMMA-"},{"id":"T12373","span":{"begin":414,"end":418},"obj":"JJ"},{"id":"T12374","span":{"begin":419,"end":423},"obj":"NN"},{"id":"T12375","span":{"begin":424,"end":427},"obj":"VB"},{"id":"T12376","span":{"begin":428,"end":439},"obj":"VB"},{"id":"T12377","span":{"begin":440,"end":442},"obj":"IN"},{"id":"T12378","span":{"begin":443,"end":446},"obj":"NNP"},{"id":"T12379","span":{"begin":447,"end":449},"obj":"TO"},{"id":"T12380","span":{"begin":450,"end":452},"obj":"DT"},{"id":"T12381","span":{"begin":453,"end":460},"obj":"JJ"},{"id":"T12382","span":{"begin":461,"end":468},"obj":"NN"},{"id":"T12384","span":{"begin":472,"end":475},"obj":"CD"},{"id":"T12385","span":{"begin":476,"end":479},"obj":"CC"},{"id":"T12386","span":{"begin":480,"end":486},"obj":"VB"},{"id":"T12387","span":{"begin":487,"end":489},"obj":"IN"},{"id":"T12388","span":{"begin":490,"end":494},"obj":"NN"},{"id":"T12389","span":{"begin":495,"end":498},"obj":"IN"},{"id":"T12390","span":{"begin":499,"end":500},"obj":"CD"},{"id":"T12391","span":{"begin":501,"end":505},"obj":"NN"},{"id":"T12392","span":{"begin":507,"end":510},"obj":"IN"},{"id":"T12393","span":{"begin":511,"end":523},"obj":"NN"},{"id":"T12394","span":{"begin":523,"end":524},"obj":"-COMMA-"},{"id":"T12395","span":{"begin":525,"end":529},"obj":"JJ"},{"id":"T12396","span":{"begin":530,"end":534},"obj":"NN"},{"id":"T12397","span":{"begin":535,"end":538},"obj":"VB"},{"id":"T12398","span":{"begin":539,"end":550},"obj":"VB"},{"id":"T12399","span":{"begin":551,"end":553},"obj":"TO"},{"id":"T12400","span":{"begin":554,"end":556},"obj":"DT"},{"id":"T12401","span":{"begin":557,"end":564},"obj":"JJ"},{"id":"T12402","span":{"begin":565,"end":572},"obj":"NN"},{"id":"T12403","span":{"begin":573,"end":575},"obj":"IN"},{"id":"T12404","span":{"begin":576,"end":579},"obj":"CD"},{"id":"T12405","span":{"begin":580,"end":582},"obj":"IN"},{"id":"T12406","span":{"begin":583,"end":586},"obj":"NNP"},{"id":"T12407","span":{"begin":587,"end":590},"obj":"CC"},{"id":"T12408","span":{"begin":591,"end":598},"obj":"RB"},{"id":"T12409","span":{"begin":599,"end":606},"obj":"VB"},{"id":"T12410","span":{"begin":607,"end":609},"obj":"IN"},{"id":"T12411","span":{"begin":610,"end":617},"obj":"NN"},{"id":"T12412","span":{"begin":618,"end":624},"obj":"NN"},{"id":"T12413","span":{"begin":625,"end":630},"obj":"JJ"},{"id":"T12414","span":{"begin":631,"end":633},"obj":"TO"},{"id":"T12415","span":{"begin":634,"end":645},"obj":"NN"},{"id":"T12416","span":{"begin":646,"end":648},"obj":"TO"},{"id":"T12417","span":{"begin":649,"end":656},"obj":"VB"},{"id":"T12418","span":{"begin":657,"end":660},"obj":"DT"},{"id":"T12419","span":{"begin":661,"end":668},"obj":"VB"},{"id":"T12420","span":{"begin":669,"end":681},"obj":"NN"},{"id":"T12421","span":{"begin":682,"end":684},"obj":"IN"},{"id":"T12422","span":{"begin":685,"end":694},"obj":"NN"},{"id":"T12423","span":{"begin":696,"end":707},"obj":"JJ"},{"id":"T12424","span":{"begin":708,"end":712},"obj":"NN"},{"id":"T12425","span":{"begin":713,"end":716},"obj":"VB"},{"id":"T12426","span":{"begin":717,"end":722},"obj":"VB"},{"id":"T12427","span":{"begin":723,"end":725},"obj":"IN"},{"id":"T12428","span":{"begin":726,"end":727},"obj":"DT"},{"id":"T12429","span":{"begin":728,"end":734},"obj":"NN"},{"id":"T12430","span":{"begin":735,"end":745},"obj":"JJ"},{"id":"T12431","span":{"begin":746,"end":748},"obj":"TO"},{"id":"T12432","span":{"begin":749,"end":753},"obj":"DT"},{"id":"T12433","span":{"begin":754,"end":758},"obj":"VB"},{"id":"T12434","span":{"begin":759,"end":761},"obj":"TO"},{"id":"T12435","span":{"begin":762,"end":769},"obj":"VB"},{"id":"T12436","span":{"begin":770,"end":772},"obj":"DT"},{"id":"T12437","span":{"begin":773,"end":776},"obj":"NN"},{"id":"T12438","span":{"begin":777,"end":779},"obj":"IN"},{"id":"T12439","span":{"begin":780,"end":781},"obj":"CD"},{"id":"T12440","span":{"begin":782,"end":785},"obj":"IN"},{"id":"T12441","span":{"begin":786,"end":790},"obj":"JJ"},{"id":"T12442","span":{"begin":791,"end":795},"obj":"NN"}],"relations":[{"id":"R10884","pred":"arg1Of","subj":"T12305","obj":"T12304"},{"id":"R10885","pred":"arg1Of","subj":"T12310","obj":"T12306"},{"id":"R10886","pred":"arg1Of","subj":"T12310","obj":"T12307"},{"id":"R10887","pred":"arg1Of","subj":"T12310","obj":"T12308"},{"id":"R10888","pred":"arg1Of","subj":"T12310","obj":"T12309"},{"id":"R10889","pred":"arg1Of","subj":"T12310","obj":"T12311"},{"id":"R10890","pred":"arg1Of","subj":"T12311","obj":"T12315"},{"id":"R10891","pred":"arg2Of","subj":"T12311","obj":"T12316"},{"id":"R10892","pred":"arg2Of","subj":"T12314","obj":"T12311"},{"id":"R10893","pred":"arg1Of","subj":"T12314","obj":"T12312"},{"id":"R10894","pred":"arg1Of","subj":"T12314","obj":"T12313"},{"id":"R10895","pred":"arg2Of","subj":"T12316","obj":"T12315"},{"id":"R10896","pred":"arg1Of","subj":"T12316","obj":"T12317"},{"id":"R10897","pred":"arg1Of","subj":"T12316","obj":"T12345"},{"id":"R10898","pred":"arg1Of","subj":"T12321","obj":"T12318"},{"id":"R10899","pred":"arg1Of","subj":"T12321","obj":"T12319"},{"id":"R10900","pred":"arg1Of","subj":"T12321","obj":"T12320"},{"id":"R10901","pred":"arg1Of","subj":"T12323","obj":"T12322"},{"id":"R10902","pred":"modOf","subj":"T12323","obj":"T12327"},{"id":"R10903","pred":"arg1Of","subj":"T12324","obj":"T12325"},{"id":"R10904","pred":"arg1Of","subj":"T12325","obj":"T12327"},{"id":"R10905","pred":"arg2Of","subj":"T12325","obj":"T12328"},{"id":"R10906","pred":"arg2Of","subj":"T12326","obj":"T12325"},{"id":"R10907","pred":"arg2Of","subj":"T12328","obj":"T12327"},{"id":"R10908","pred":"arg1Of","subj":"T12328","obj":"T12329"},{"id":"R10909","pred":"arg2Of","subj":"T12331","obj":"T12329"},{"id":"R10910","pred":"arg1Of","subj":"T12331","obj":"T12330"},{"id":"R10911","pred":"arg1Of","subj":"T12331","obj":"T12332"},{"id":"R10912","pred":"arg2Of","subj":"T12334","obj":"T12332"},{"id":"R10913","pred":"arg1Of","subj":"T12334","obj":"T12333"},{"id":"R10914","pred":"arg2Of","subj":"T12336","obj":"T12335"},{"id":"R10915","pred":"arg3Of","subj":"T12337","obj":"T12335"},{"id":"R10916","pred":"arg1Of","subj":"T12338","obj":"T12335"},{"id":"R10917","pred":"arg1Of","subj":"T12338","obj":"T12339"},{"id":"R10918","pred":"arg2Of","subj":"T12339","obj":"T12317"},{"id":"R10919","pred":"arg1Of","subj":"T12339","obj":"T12321"},{"id":"R10920","pred":"arg1Of","subj":"T12339","obj":"T12323"},{"id":"R10921","pred":"arg2Of","subj":"T12340","obj":"T12339"},{"id":"R10922","pred":"arg1Of","subj":"T12340","obj":"T12341"},{"id":"R10923","pred":"arg2Of","subj":"T12343","obj":"T12341"},{"id":"R10924","pred":"arg1Of","subj":"T12343","obj":"T12342"},{"id":"R10925","pred":"arg3Of","subj":"T12344","obj":"T12341"},{"id":"R10926","pred":"arg2Of","subj":"T12347","obj":"T12345"},{"id":"R10927","pred":"arg1Of","subj":"T12347","obj":"T12346"},{"id":"R10928","pred":"arg1Of","subj":"T12348","obj":"T12349"},{"id":"R10929","pred":"arg2Of","subj":"T12348","obj":"T12350"},{"id":"R10930","pred":"arg1Of","subj":"T12348","obj":"T12355"},{"id":"R10931","pred":"arg2Of","subj":"T12350","obj":"T12349"},{"id":"R10932","pred":"arg1Of","subj":"T12350","obj":"T12351"},{"id":"R10933","pred":"arg1Of","subj":"T12350","obj":"T12352"},{"id":"R10934","pred":"arg1Of","subj":"T12350","obj":"T12354"},{"id":"R10935","pred":"arg2Of","subj":"T12353","obj":"T12352"},{"id":"R10936","pred":"arg2Of","subj":"T12355","obj":"T12354"},{"id":"R10937","pred":"arg1Of","subj":"T12355","obj":"T12356"},{"id":"R10938","pred":"arg1Of","subj":"T12355","obj":"T12358"},{"id":"R10939","pred":"arg2Of","subj":"T12357","obj":"T12356"},{"id":"R10940","pred":"arg2Of","subj":"T12361","obj":"T12358"},{"id":"R10941","pred":"arg1Of","subj":"T12361","obj":"T12359"},{"id":"R10942","pred":"arg1Of","subj":"T12361","obj":"T12360"},{"id":"R10943","pred":"arg1Of","subj":"T12361","obj":"T12362"},{"id":"R10944","pred":"arg2Of","subj":"T12363","obj":"T12362"},{"id":"R10945","pred":"arg1Of","subj":"T12363","obj":"T12364"},{"id":"R10946","pred":"arg2Of","subj":"T12366","obj":"T12364"},{"id":"R10947","pred":"arg1Of","subj":"T12366","obj":"T12365"},{"id":"R10948","pred":"arg3Of","subj":"T12367","obj":"T12364"},{"id":"R10949","pred":"arg1Of","subj":"T12369","obj":"T12368"},{"id":"R10950","pred":"arg2Of","subj":"T12371","obj":"T12369"},{"id":"R10951","pred":"arg1Of","subj":"T12371","obj":"T12370"},{"id":"R10952","pred":"arg1Of","subj":"T12374","obj":"T12369"},{"id":"R10953","pred":"arg1Of","subj":"T12374","obj":"T12373"},{"id":"R10954","pred":"arg1Of","subj":"T12374","obj":"T12375"},{"id":"R10955","pred":"arg2Of","subj":"T12374","obj":"T12376"},{"id":"R10956","pred":"arg2Of","subj":"T12374","obj":"T12386"},{"id":"R10957","pred":"arg1Of","subj":"T12376","obj":"T12377"},{"id":"R10958","pred":"arg1Of","subj":"T12376","obj":"T12379"},{"id":"R10959","pred":"arg1Of","subj":"T12376","obj":"T12385"},{"id":"R10960","pred":"arg2Of","subj":"T12378","obj":"T12377"},{"id":"R10961","pred":"arg2Of","subj":"T12382","obj":"T12379"},{"id":"R10962","pred":"arg1Of","subj":"T12382","obj":"T12380"},{"id":"R10963","pred":"arg1Of","subj":"T12382","obj":"T12381"},{"id":"R10964","pred":"arg1Of","subj":"T12382","obj":"T12383"},{"id":"R10965","pred":"arg2Of","subj":"T12384","obj":"T12383"},{"id":"R10966","pred":"modOf","subj":"T12385","obj":"T12368"},{"id":"R10967","pred":"arg1Of","subj":"T12385","obj":"T12372"},{"id":"R10968","pred":"arg2Of","subj":"T12385","obj":"T12375"},{"id":"R10969","pred":"arg2Of","subj":"T12386","obj":"T12385"},{"id":"R10970","pred":"arg1Of","subj":"T12386","obj":"T12387"},{"id":"R10971","pred":"arg1Of","subj":"T12386","obj":"T12389"},{"id":"R10972","pred":"arg2Of","subj":"T12388","obj":"T12387"},{"id":"R10973","pred":"arg2Of","subj":"T12391","obj":"T12389"},{"id":"R10974","pred":"arg1Of","subj":"T12391","obj":"T12390"},{"id":"R10975","pred":"arg2Of","subj":"T12393","obj":"T12392"},{"id":"R10976","pred":"arg1Of","subj":"T12396","obj":"T12395"},{"id":"R10977","pred":"arg1Of","subj":"T12396","obj":"T12397"},{"id":"R10978","pred":"arg2Of","subj":"T12396","obj":"T12398"},{"id":"R10979","pred":"arg2Of","subj":"T12396","obj":"T12409"},{"id":"R10980","pred":"arg2Of","subj":"T12398","obj":"T12397"},{"id":"R10981","pred":"arg1Of","subj":"T12398","obj":"T12399"},{"id":"R10982","pred":"arg1Of","subj":"T12398","obj":"T12407"},{"id":"R10983","pred":"arg1Of","subj":"T12399","obj":"T12405"},{"id":"R10984","pred":"arg2Of","subj":"T12402","obj":"T12399"},{"id":"R10985","pred":"arg1Of","subj":"T12402","obj":"T12400"},{"id":"R10986","pred":"arg1Of","subj":"T12402","obj":"T12401"},{"id":"R10987","pred":"arg1Of","subj":"T12402","obj":"T12403"},{"id":"R10988","pred":"arg2Of","subj":"T12404","obj":"T12403"},{"id":"R10989","pred":"arg2Of","subj":"T12406","obj":"T12405"},{"id":"R10990","pred":"arg1Of","subj":"T12407","obj":"T12392"},{"id":"R10991","pred":"arg1Of","subj":"T12407","obj":"T12394"},{"id":"R10992","pred":"arg2Of","subj":"T12409","obj":"T12407"},{"id":"R10993","pred":"arg1Of","subj":"T12409","obj":"T12408"},{"id":"R10994","pred":"arg1Of","subj":"T12409","obj":"T12410"},{"id":"R10995","pred":"arg2Of","subj":"T12412","obj":"T12410"},{"id":"R10996","pred":"arg1Of","subj":"T12412","obj":"T12411"},{"id":"R10997","pred":"arg1Of","subj":"T12412","obj":"T12413"},{"id":"R10998","pred":"arg1Of","subj":"T12413","obj":"T12414"},{"id":"R10999","pred":"arg2Of","subj":"T12415","obj":"T12414"},{"id":"R11000","pred":"modOf","subj":"T12415","obj":"T12416"},{"id":"R11001","pred":"arg1Of","subj":"T12417","obj":"T12416"},{"id":"R11002","pred":"arg2Of","subj":"T12420","obj":"T12417"},{"id":"R11003","pred":"arg1Of","subj":"T12420","obj":"T12418"},{"id":"R11004","pred":"arg2Of","subj":"T12420","obj":"T12419"},{"id":"R11005","pred":"arg1Of","subj":"T12420","obj":"T12421"},{"id":"R11006","pred":"arg2Of","subj":"T12422","obj":"T12421"},{"id":"R11007","pred":"arg1Of","subj":"T12424","obj":"T12423"},{"id":"R11008","pred":"arg1Of","subj":"T12424","obj":"T12425"},{"id":"R11009","pred":"arg2Of","subj":"T12424","obj":"T12426"},{"id":"R11010","pred":"arg2Of","subj":"T12426","obj":"T12425"},{"id":"R11011","pred":"arg1Of","subj":"T12426","obj":"T12427"},{"id":"R11012","pred":"arg2Of","subj":"T12429","obj":"T12427"},{"id":"R11013","pred":"arg1Of","subj":"T12429","obj":"T12428"},{"id":"R11014","pred":"arg1Of","subj":"T12429","obj":"T12430"},{"id":"R11015","pred":"arg1Of","subj":"T12430","obj":"T12431"},{"id":"R11016","pred":"arg2Of","subj":"T12432","obj":"T12431"},{"id":"R11017","pred":"arg2Of","subj":"T12432","obj":"T12433"},{"id":"R11018","pred":"modOf","subj":"T12433","obj":"T12434"},{"id":"R11019","pred":"arg1Of","subj":"T12435","obj":"T12434"},{"id":"R11020","pred":"arg2Of","subj":"T12437","obj":"T12435"},{"id":"R11021","pred":"arg1Of","subj":"T12437","obj":"T12436"},{"id":"R11022","pred":"arg1Of","subj":"T12437","obj":"T12438"},{"id":"R11023","pred":"arg1Of","subj":"T12437","obj":"T12440"},{"id":"R11024","pred":"arg2Of","subj":"T12439","obj":"T12438"},{"id":"R11025","pred":"arg2Of","subj":"T12442","obj":"T12440"},{"id":"R11026","pred":"arg1Of","subj":"T12442","obj":"T12441"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"Bacterial strains\nP. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase. Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm). To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour. For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection. Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.\n\n"}
sentences
{"project":"sentences","denotations":[{"id":"T12298","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T12299","span":{"begin":18,"end":284},"obj":"Sentence"},{"id":"T12300","span":{"begin":285,"end":380},"obj":"Sentence"},{"id":"T12301","span":{"begin":381,"end":506},"obj":"Sentence"},{"id":"T12302","span":{"begin":507,"end":695},"obj":"Sentence"},{"id":"T12303","span":{"begin":696,"end":796},"obj":"Sentence"},{"id":"T229","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T230","span":{"begin":18,"end":119},"obj":"Sentence"},{"id":"T231","span":{"begin":120,"end":284},"obj":"Sentence"},{"id":"T232","span":{"begin":285,"end":380},"obj":"Sentence"},{"id":"T233","span":{"begin":381,"end":506},"obj":"Sentence"},{"id":"T234","span":{"begin":507,"end":695},"obj":"Sentence"},{"id":"T235","span":{"begin":696,"end":796},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Bacterial strains\nP. aeruginosa laboratory strains PAO1 and the PAO1ΔexsA mutant were obtained from Dr. Robert Hancock. P. aeruginosa strains PAO1 and PAO1ΔexsA were grown from overnight cultures in Luria Bertani (LB) broth and LB+streptomycin (150 µg/ml) until mid-logarithmic phase. Cells were washed once in PBS and resuspended in PBS to an optical density of 0.5 (λ = 600 nm). To prepare heat-killed bacteria, live PAO1 was resuspended in PBS to an optical density of 0.5 and heated at 60°C for 1 hour. For stimulations, live PAO1 was resuspended to an optical density of 0.5 in PBS and further diluted in culture medium prior to stimulation to achieve the desired multiplicity of infection. Heat-killed PAO1 was added in a volume equivalent to that used to achieve an MOI of 1 for live PAO1.\n\n"}