PMC:3359311 / 16297-17810 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5633","span":{"begin":46,"end":51},"obj":"P01584"},{"id":"T5634","span":{"begin":256,"end":261},"obj":"P01584"},{"id":"T5635","span":{"begin":469,"end":474},"obj":"P01584"},{"id":"T5636","span":{"begin":478,"end":482},"obj":"P10145"},{"id":"T5637","span":{"begin":553,"end":558},"obj":"P01584"},{"id":"T5638","span":{"begin":681,"end":685},"obj":"P10145"},{"id":"T14806","span":{"begin":879,"end":884},"obj":"P01584"},{"id":"T14807","span":{"begin":1076,"end":1081},"obj":"P01584"},{"id":"T14808","span":{"begin":1090,"end":1094},"obj":"P10145"},{"id":"T14809","span":{"begin":1294,"end":1299},"obj":"P01584"},{"id":"T14810","span":{"begin":1435,"end":1440},"obj":"P01584"},{"id":"T14811","span":{"begin":1456,"end":1460},"obj":"P10145"},{"id":"T14812","span":{"begin":1476,"end":1480},"obj":"P05412"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T5486","span":{"begin":37,"end":45},"obj":"Positive_regulation"},{"id":"T5487","span":{"begin":46,"end":51},"obj":"Protein"},{"id":"T5488","span":{"begin":52,"end":62},"obj":"Gene_expression"},{"id":"T5489","span":{"begin":249,"end":255},"obj":"Regulation"},{"id":"T5490","span":{"begin":256,"end":261},"obj":"Protein"},{"id":"T5491","span":{"begin":262,"end":272},"obj":"Gene_expression"},{"id":"T5492","span":{"begin":463,"end":468},"obj":"Regulation"},{"id":"T5493","span":{"begin":469,"end":474},"obj":"Protein"},{"id":"T5494","span":{"begin":483,"end":493},"obj":"Gene_expression"},{"id":"T5495","span":{"begin":553,"end":558},"obj":"Protein"},{"id":"T5496","span":{"begin":559,"end":569},"obj":"Gene_expression"},{"id":"T14661","span":{"begin":870,"end":878},"obj":"Positive_regulation"},{"id":"T14662","span":{"begin":879,"end":884},"obj":"Protein"},{"id":"T14663","span":{"begin":885,"end":895},"obj":"Gene_expression"},{"id":"T14664","span":{"begin":1076,"end":1081},"obj":"Protein"},{"id":"T14665","span":{"begin":1095,"end":1105},"obj":"Gene_expression"},{"id":"T14666","span":{"begin":1294,"end":1299},"obj":"Protein"},{"id":"T14667","span":{"begin":1300,"end":1310},"obj":"Gene_expression"},{"id":"T14668","span":{"begin":1435,"end":1440},"obj":"Protein"},{"id":"T14669","span":{"begin":1441,"end":1450},"obj":"Localization"}],"relations":[{"id":"R4746","pred":"themeOf","subj":"T5487","obj":"T5488"},{"id":"R4747","pred":"themeOf","subj":"T5488","obj":"T5486"},{"id":"R4748","pred":"themeOf","subj":"T5490","obj":"T5491"},{"id":"R4749","pred":"themeOf","subj":"T5491","obj":"T5489"},{"id":"R4750","pred":"themeOf","subj":"T5493","obj":"T5494"},{"id":"R4751","pred":"themeOf","subj":"T5494","obj":"T5492"},{"id":"R4752","pred":"themeOf","subj":"T5495","obj":"T5496"},{"id":"R13025","pred":"themeOf","subj":"T14662","obj":"T14663"},{"id":"R13026","pred":"themeOf","subj":"T14663","obj":"T14661"},{"id":"R13027","pred":"themeOf","subj":"T14664","obj":"T14665"},{"id":"R13028","pred":"themeOf","subj":"T14666","obj":"T14667"},{"id":"R13029","pred":"themeOf","subj":"T14668","obj":"T14669"}],"attributes":[{"id":"M207","pred":"Speculation","subj":"T14669","obj":"true"},{"id":"M204","pred":"Negation","subj":"T14661","obj":"true"},{"id":"M94","pred":"Negation","subj":"T5492","obj":"true"},{"id":"M205","pred":"Speculation","subj":"T14665","obj":"true"},{"id":"M93","pred":"Speculation","subj":"T5489","obj":"true"},{"id":"M92","pred":"Negation","subj":"T5486","obj":"true"},{"id":"M206","pred":"Speculation","subj":"T14667","obj":"true"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T5823","span":{"begin":14,"end":18},"obj":"Protein"},{"id":"T5824","span":{"begin":46,"end":51},"obj":"Protein"},{"id":"T5825","span":{"begin":0,"end":10},"obj":"Negative_regulation"},{"id":"T5826","span":{"begin":52,"end":62},"obj":"Gene_expression"},{"id":"T5827","span":{"begin":37,"end":45},"obj":"Positive_regulation"},{"id":"T5828","span":{"begin":175,"end":193},"obj":"Protein"},{"id":"T5829","span":{"begin":232,"end":236},"obj":"Protein"},{"id":"T5830","span":{"begin":256,"end":261},"obj":"Protein"},{"id":"T5831","span":{"begin":356,"end":360},"obj":"Protein"},{"id":"T5832","span":{"begin":237,"end":248},"obj":"Positive_regulation"},{"id":"T5833","span":{"begin":262,"end":272},"obj":"Gene_expression"},{"id":"T5834","span":{"begin":249,"end":255},"obj":"Positive_regulation"},{"id":"T5835","span":{"begin":469,"end":474},"obj":"Protein"},{"id":"T5836","span":{"begin":478,"end":482},"obj":"Protein"},{"id":"T5837","span":{"begin":483,"end":493},"obj":"Gene_expression"},{"id":"T5838","span":{"begin":483,"end":493},"obj":"Gene_expression"},{"id":"T5839","span":{"begin":463,"end":468},"obj":"Regulation"},{"id":"T5840","span":{"begin":463,"end":468},"obj":"Regulation"},{"id":"T5841","span":{"begin":553,"end":558},"obj":"Protein"},{"id":"T5842","span":{"begin":657,"end":667},"obj":"Protein"},{"id":"T5843","span":{"begin":669,"end":672},"obj":"Protein"},{"id":"T5844","span":{"begin":559,"end":569},"obj":"Gene_expression"},{"id":"T5845","span":{"begin":681,"end":685},"obj":"Protein"},{"id":"T5846","span":{"begin":687,"end":694},"obj":"Protein"},{"id":"T5847","span":{"begin":700,"end":705},"obj":"Protein"},{"id":"T5848","span":{"begin":716,"end":723},"obj":"Protein"},{"id":"T15001","span":{"begin":847,"end":851},"obj":"Protein"},{"id":"T15002","span":{"begin":879,"end":884},"obj":"Protein"},{"id":"T15003","span":{"begin":833,"end":843},"obj":"Negative_regulation"},{"id":"T15004","span":{"begin":885,"end":895},"obj":"Gene_expression"},{"id":"T15005","span":{"begin":870,"end":878},"obj":"Positive_regulation"},{"id":"T15006","span":{"begin":1056,"end":1060},"obj":"Protein"},{"id":"T15007","span":{"begin":1236,"end":1246},"obj":"Protein"},{"id":"T15008","span":{"begin":1294,"end":1299},"obj":"Protein"},{"id":"T15009","span":{"begin":1300,"end":1310},"obj":"Gene_expression"},{"id":"T15010","span":{"begin":1409,"end":1413},"obj":"Protein"},{"id":"T15011","span":{"begin":1473,"end":1475},"obj":"Protein"},{"id":"T15012","span":{"begin":1476,"end":1480},"obj":"Protein"}],"relations":[{"id":"R5050","pred":"themeOf","subj":"T5835","obj":"T5837"},{"id":"R5051","pred":"themeOf","subj":"T5836","obj":"T5838"},{"id":"R5042","pred":"themeOf","subj":"T5823","obj":"T5825"},{"id":"R5043","pred":"themeOf","subj":"T5824","obj":"T5826"},{"id":"R5044","pred":"causeOf","subj":"T5825","obj":"T5827"},{"id":"R5045","pred":"themeOf","subj":"T5826","obj":"T5827"},{"id":"R5046","pred":"themeOf","subj":"T5829","obj":"T5832"},{"id":"R5047","pred":"themeOf","subj":"T5830","obj":"T5833"},{"id":"R5048","pred":"causeOf","subj":"T5832","obj":"T5834"},{"id":"R5049","pred":"themeOf","subj":"T5833","obj":"T5834"},{"id":"R5052","pred":"themeOf","subj":"T5837","obj":"T5839"},{"id":"R5053","pred":"themeOf","subj":"T5838","obj":"T5840"},{"id":"R5054","pred":"themeOf","subj":"T5841","obj":"T5844"},{"id":"R13332","pred":"themeOf","subj":"T15001","obj":"T15003"},{"id":"R13333","pred":"themeOf","subj":"T15002","obj":"T15004"},{"id":"R13334","pred":"causeOf","subj":"T15003","obj":"T15005"},{"id":"R13335","pred":"themeOf","subj":"T15004","obj":"T15005"},{"id":"R13336","pred":"themeOf","subj":"T15008","obj":"T15009"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"events-check-again","denotations":[{"id":"T5812","span":{"begin":37,"end":45},"obj":"Positive_regulation"},{"id":"T5813","span":{"begin":46,"end":51},"obj":"Protein"},{"id":"T5814","span":{"begin":52,"end":62},"obj":"Gene_expression"},{"id":"T5815","span":{"begin":249,"end":255},"obj":"Regulation"},{"id":"T5816","span":{"begin":256,"end":261},"obj":"Protein"},{"id":"T5817","span":{"begin":262,"end":272},"obj":"Gene_expression"},{"id":"T5818","span":{"begin":463,"end":468},"obj":"Regulation"},{"id":"T5819","span":{"begin":469,"end":474},"obj":"Protein"},{"id":"T5820","span":{"begin":483,"end":493},"obj":"Gene_expression"},{"id":"T5821","span":{"begin":553,"end":558},"obj":"Protein"},{"id":"T5822","span":{"begin":559,"end":569},"obj":"Gene_expression"},{"id":"T14992","span":{"begin":870,"end":878},"obj":"Positive_regulation"},{"id":"T14993","span":{"begin":879,"end":884},"obj":"Protein"},{"id":"T14994","span":{"begin":885,"end":895},"obj":"Gene_expression"},{"id":"T14995","span":{"begin":1076,"end":1081},"obj":"Protein"},{"id":"T14996","span":{"begin":1095,"end":1105},"obj":"Gene_expression"},{"id":"T14997","span":{"begin":1294,"end":1299},"obj":"Protein"},{"id":"T14998","span":{"begin":1300,"end":1310},"obj":"Gene_expression"},{"id":"T14999","span":{"begin":1435,"end":1440},"obj":"Protein"},{"id":"T15000","span":{"begin":1441,"end":1450},"obj":"Localization"}],"relations":[{"id":"R5035","pred":"themeOf","subj":"T5813","obj":"T5814"},{"id":"R5036","pred":"themeOf","subj":"T5814","obj":"T5812"},{"id":"R5037","pred":"themeOf","subj":"T5816","obj":"T5817"},{"id":"R5038","pred":"themeOf","subj":"T5817","obj":"T5815"},{"id":"R5039","pred":"themeOf","subj":"T5819","obj":"T5820"},{"id":"R5040","pred":"themeOf","subj":"T5820","obj":"T5818"},{"id":"R5041","pred":"themeOf","subj":"T5821","obj":"T5822"},{"id":"R13327","pred":"themeOf","subj":"T14993","obj":"T14994"},{"id":"R13328","pred":"themeOf","subj":"T14994","obj":"T14992"},{"id":"R13329","pred":"themeOf","subj":"T14995","obj":"T14996"},{"id":"R13330","pred":"themeOf","subj":"T14997","obj":"T14998"},{"id":"R13331","pred":"themeOf","subj":"T14999","obj":"T15000"}],"attributes":[{"id":"M103","pred":"Negation","subj":"T5818","obj":"true"},{"id":"M102","pred":"Speculation","subj":"T5815","obj":"true"},{"id":"M216","pred":"Negation","subj":"T14992","obj":"true"},{"id":"M218","pred":"Speculation","subj":"T14998","obj":"true"},{"id":"M217","pred":"Speculation","subj":"T14996","obj":"true"},{"id":"M219","pred":"Speculation","subj":"T15000","obj":"true"},{"id":"M101","pred":"Negation","subj":"T5812","obj":"true"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"GO-CC","denotations":[{"id":"T5786","span":{"begin":82,"end":87},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5787","span":{"begin":280,"end":285},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5788","span":{"begin":638,"end":643},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5789","span":{"begin":782,"end":787},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14971","span":{"begin":1339,"end":1344},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"GO-MF","denotations":[{"id":"T5784","span":{"begin":478,"end":482},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T5785","span":{"begin":681,"end":685},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T14972","span":{"begin":1090,"end":1094},"obj":"http://purl.obolibrary.org/obo/GO_0005153"},{"id":"T14973","span":{"begin":1456,"end":1460},"obj":"http://purl.obolibrary.org/obo/GO_0005153"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"GO-BP","denotations":[{"id":"T5782","span":{"begin":478,"end":493},"obj":"http://purl.obolibrary.org/obo/GO_0032677"},{"id":"T5783","span":{"begin":478,"end":493},"obj":"http://purl.obolibrary.org/obo/GO_0032637"},{"id":"T14967","span":{"begin":1090,"end":1105},"obj":"http://purl.obolibrary.org/obo/GO_0032677"},{"id":"T14968","span":{"begin":1090,"end":1105},"obj":"http://purl.obolibrary.org/obo/GO_0032637"},{"id":"T14969","span":{"begin":1173,"end":1204},"obj":"http://purl.obolibrary.org/obo/GO_0030225"},{"id":"T14970","span":{"begin":1441,"end":1450},"obj":"http://purl.obolibrary.org/obo/GO_0046903"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

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of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"sentences","denotations":[{"id":"T5497","span":{"begin":0,"end":87},"obj":"Sentence"},{"id":"T5498","span":{"begin":88,"end":210},"obj":"Sentence"},{"id":"T5499","span":{"begin":211,"end":428},"obj":"Sentence"},{"id":"T5500","span":{"begin":429,"end":541},"obj":"Sentence"},{"id":"T5501","span":{"begin":542,"end":680},"obj":"Sentence"},{"id":"T5502","span":{"begin":681,"end":788},"obj":"Sentence"},{"id":"T14670","span":{"begin":833,"end":920},"obj":"Sentence"},{"id":"T14671","span":{"begin":921,"end":1205},"obj":"Sentence"},{"id":"T14672","span":{"begin":1206,"end":1323},"obj":"Sentence"},{"id":"T14673","span":{"begin":1324,"end":1510},"obj":"Sentence"},{"id":"T113","span":{"begin":0,"end":87},"obj":"Sentence"},{"id":"T114","span":{"begin":88,"end":210},"obj":"Sentence"},{"id":"T115","span":{"begin":211,"end":428},"obj":"Sentence"},{"id":"T116","span":{"begin":429,"end":541},"obj":"Sentence"},{"id":"T117","span":{"begin":542,"end":680},"obj":"Sentence"},{"id":"T118","span":{"begin":681,"end":788},"obj":"Sentence"},{"id":"T119","span":{"begin":789,"end":920},"obj":"Sentence"},{"id":"T120","span":{"begin":921,"end":1205},"obj":"Sentence"},{"id":"T121","span":{"begin":1206,"end":1323},"obj":"Sentence"},{"id":"T122","span":{"begin":1324,"end":1510},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}

{"project":"2_test","denotations":[{"id":"22649552-16301672-88306715","span":{"begin":206,"end":208},"obj":"16301672"},{"id":"T11221","span":{"begin":206,"end":208},"obj":"16301672"}],"text":"Disruption of CFTR activity does not increase IL-1β production in PBMCs and THP-1 cells\nA previous study has indicated a role for chloride ion concentration in suppression of NLRP3 inflammasome activation [34]. To determine whether CFTR dysfunction alters IL-1β production, THP-1 cells and PBMCs from CF patients and healthy controls were treated with the CFTR inhibitor, CFTRinh172, prior to simulation with live P. aeruginosa. Treatment with CFTRinh172 did not alter IL-1β or IL-8 production in control subjects or CF patients (Fig. 8a–b). Similarly, IL-1β production was not different in monocyte-derived macrophages or THP-1 reporter cells treated with CFTRinh172 (Fig. 8c–d). IL-8 (Fig. 8e) and NF-κB activity (Fig. 8f) were also unchanged in CFTRinh172-treated THP-1 reporter cells.\n10.1371/journal.pone.0037689.g008 Figure 8 Disruption of CFTR activity does not increase IL-1β production in PBMCs or macrophages.\nPBMCs from CF patients (n = 15) and controls (n = 13) were treated with CFTRinh172 (10 µM) for 18 hours prior to stimulation with live PAO1 (MOI = 1). (A) IL-1β and (B) IL-8 production was measured at 24 hours. (C) Monocytes from controls (n = 3) were differentiated into macrophages. Macrophages were treated with CFTRinh172, stimulated as per monocytes, and measured for IL-1β production at 24 hours. THP-1 reporter cells were treated with CFTRinh172 24 hours prior to stimulation with PAO1 and measured for (D) IL-1β secretion, (E) IL-8, and (F) NF-κB/AP-1 activity at 24 hours (n = 4).\n\nD"}