PMC:3342329 / 9672-11226 JSONTXT

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of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T17770","span":{"begin":1505,"end":1509},"obj":"Protein"},{"id":"T17769","span":{"begin":1399,"end":1403},"obj":"Protein"},{"id":"T17768","span":{"begin":1356,"end":1359},"obj":"Protein"},{"id":"T17767","span":{"begin":1297,"end":1300},"obj":"Protein"},{"id":"T17766","span":{"begin":1132,"end":1135},"obj":"Protein"},{"id":"T17765","span":{"begin":1124,"end":1127},"obj":"Protein"},{"id":"T17764","span":{"begin":1037,"end":1041},"obj":"Protein"},{"id":"T17763","span":{"begin":1020,"end":1024},"obj":"Protein"},{"id":"T17762","span":{"begin":46,"end":50},"obj":"Protein"},{"id":"T5450","span":{"begin":807,"end":811},"obj":"Protein"},{"id":"T5449","span":{"begin":765,"end":768},"obj":"Protein"},{"id":"T5448","span":{"begin":653,"end":657},"obj":"Protein"},{"id":"T5447","span":{"begin":540,"end":543},"obj":"Protein"},{"id":"T5446","span":{"begin":530,"end":533},"obj":"Protein"},{"id":"T5445","span":{"begin":460,"end":464},"obj":"Protein"},{"id":"T5444","span":{"begin":170,"end":174},"obj":"Protein"},{"id":"T5443","span":{"begin":46,"end":50},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T18079","span":{"begin":1132,"end":1135},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T18078","span":{"begin":1132,"end":1135},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T18077","span":{"begin":1356,"end":1359},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T18076","span":{"begin":1297,"end":1300},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T18075","span":{"begin":1124,"end":1127},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T18074","span":{"begin":1505,"end":1509},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T18073","span":{"begin":1399,"end":1403},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T18072","span":{"begin":1037,"end":1041},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T18071","span":{"begin":1020,"end":1024},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T18070","span":{"begin":46,"end":50},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T5799","span":{"begin":765,"end":768},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T5798","span":{"begin":530,"end":533},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T5797","span":{"begin":807,"end":811},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T5796","span":{"begin":653,"end":657},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T5795","span":{"begin":460,"end":464},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T5794","span":{"begin":170,"end":174},"obj":"http://www.uniprot.org/uniprot/P51681"},{"id":"T5793","span":{"begin":46,"end":50},"obj":"http://www.uniprot.org/uniprot/P51681"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"UBERON-AE","denotations":[{"id":"T17743","span":{"begin":1388,"end":1395},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T17742","span":{"begin":1056,"end":1063},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T17741","span":{"begin":36,"end":42},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5417","span":{"begin":796,"end":803},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5416","span":{"begin":642,"end":649},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5415","span":{"begin":479,"end":485},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5414","span":{"begin":383,"end":389},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T5413","span":{"begin":36,"end":42},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"GO-BP","denotations":[{"id":"T17773","span":{"begin":1190,"end":1209},"obj":"http://purl.obolibrary.org/obo/GO_0007252"},{"id":"T17772","span":{"begin":1194,"end":1209},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T17771","span":{"begin":1136,"end":1151},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T5457","span":{"begin":587,"end":609},"obj":"http://purl.obolibrary.org/obo/GO_0007252"},{"id":"T5456","span":{"begin":587,"end":602},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T5455","span":{"begin":573,"end":609},"obj":"http://purl.obolibrary.org/obo/GO_1903720"},{"id":"T5454","span":{"begin":573,"end":602},"obj":"http://purl.obolibrary.org/obo/GO_0042326"},{"id":"T5453","span":{"begin":410,"end":431},"obj":"http://purl.obolibrary.org/obo/GO_1904744"},{"id":"T5452","span":{"begin":410,"end":431},"obj":"http://purl.obolibrary.org/obo/GO_0043388"},{"id":"T5451","span":{"begin":63,"end":82},"obj":"http://purl.obolibrary.org/obo/GO_0051092"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"GO-MF","denotations":[{"id":"T17776","span":{"begin":948,"end":955},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T17775","span":{"begin":944,"end":955},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T5464","span":{"begin":424,"end":431},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5463","span":{"begin":292,"end":299},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5462","span":{"begin":420,"end":431},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T5461","span":{"begin":288,"end":299},"obj":"http://purl.obolibrary.org/obo/GO_0003677"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"GO-CC","denotations":[{"id":"T5460","span":{"begin":553,"end":560},"obj":"http://purl.obolibrary.org/obo/GO_0005634"},{"id":"T5459","span":{"begin":157,"end":162},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5458","span":{"begin":115,"end":119},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T17774","span":{"begin":1217,"end":1224},"obj":"http://purl.obolibrary.org/obo/GO_0005829"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

{"project":"sentences","denotations":[{"id":"T17749","span":{"begin":1528,"end":1554},"obj":"Sentence"},{"id":"T17748","span":{"begin":1413,"end":1527},"obj":"Sentence"},{"id":"T17747","span":{"begin":1262,"end":1412},"obj":"Sentence"},{"id":"T17746","span":{"begin":1107,"end":1261},"obj":"Sentence"},{"id":"T17745","span":{"begin":937,"end":1106},"obj":"Sentence"},{"id":"T17744","span":{"begin":0,"end":186},"obj":"Sentence"},{"id":"T5423","span":{"begin":679,"end":832},"obj":"Sentence"},{"id":"T5422","span":{"begin":499,"end":678},"obj":"Sentence"},{"id":"T5421","span":{"begin":391,"end":498},"obj":"Sentence"},{"id":"T5420","span":{"begin":187,"end":390},"obj":"Sentence"},{"id":"T5419","span":{"begin":59,"end":186},"obj":"Sentence"},{"id":"T5418","span":{"begin":0,"end":58},"obj":"Sentence"},{"id":"T76","span":{"begin":0,"end":58},"obj":"Sentence"},{"id":"T77","span":{"begin":59,"end":186},"obj":"Sentence"},{"id":"T78","span":{"begin":187,"end":390},"obj":"Sentence"},{"id":"T79","span":{"begin":391,"end":498},"obj":"Sentence"},{"id":"T80","span":{"begin":499,"end":678},"obj":"Sentence"},{"id":"T81","span":{"begin":679,"end":832},"obj":"Sentence"},{"id":"T82","span":{"begin":833,"end":936},"obj":"Sentence"},{"id":"T83","span":{"begin":937,"end":1106},"obj":"Sentence"},{"id":"T84","span":{"begin":1107,"end":1261},"obj":"Sentence"},{"id":"T85","span":{"begin":1262,"end":1412},"obj":"Sentence"},{"id":"T86","span":{"begin":1413,"end":1527},"obj":"Sentence"},{"id":"T87","span":{"begin":1528,"end":1554},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Decrease of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

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of NF-κB Activity in Tumor Tissue of CCR5−/− Mice\nThe activation of NF-κB plays a critical role in cancer cell survival, especially melanoma cancer cells and in CCR5 expression. To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}

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To evaluate whether the tumor inhibition was related with the inactivation of NF-κB in melanoma, the DNA binding activity of NF-κB was determined by electromobility shift assay (EMSA) in melanoma tissue. The constitutively activated DNA binding activity was reduced in the CCR5−/− mice tumor tissue (Figure 2A). Moreover, the translocation of p50 and p-p65 into the nucleus through the inhibition of phosphorylation of IκB was also prevented in the tumor tissues of CCR5−/− mice (Figure 2B). Immunofluorescence analysis also confirmed that the intensity of nuclear staining for p50 had decreased in the tumor tissues of CCR5−/− mice (Figure 2C).\n10.1371/journal.pone.0033747.g002 Figure 2 Decrease of NF-κB activity in tumor tissue of CCR5−/− mice.\nA, The DNA binding activity of NF-κB was determined in the nuclear extracts of the CCR5−/− mice and CCR5+/+ mice tumor tissues by EMSA described in material and methods. B, Expression of p50 and p65 phosphorylation in nuclear extracts (NE), and IκB and IκB phosphorylation in the cytosol (CE) determined by Western blotting. C, Immunolfluorescence analysis of p50 confirmed that the intensities of nuclear staining for p50 were decreased in the tumor tissues of CCR5−/− mice. Data are means ± S.D. of four experimental animals. * indicates significant difference from CCR5+/+ mice (p\u003c0.05). Scale bar indicates 50 µm."}