PMC:3342329 / 34915-35453 JSONTXT

Annnotations TAB JSON ListView MergeView

    pmc-enju-pas

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tumor and spleen tissues were excised and homogenized in PBS with protease inhibitor cocktail (Sigma-Aldrich, USA) and Triton X-100 (final concentration 1%). The samples were frozen at −70°C, thawed, and centrifuged at 10,000×g for 5 min to remove cellular debris. The tissue lysates were performed protein assay in the same way as for western blotting. 4.5 mg of proteins, collected from three samples per group, were used for Mouse cytokine array as the protocol provided by supplier (Proteome Profiler™, #ARY006, R\u0026D Systems, USA)."}

    UBERON-AE

    {"project":"UBERON-AE","denotations":[{"id":"T16727","span":{"begin":273,"end":279},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T16726","span":{"begin":21,"end":28},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T16725","span":{"begin":14,"end":20},"obj":"http://purl.obolibrary.org/obo/UBERON_0002106"}],"text":"The tumor and spleen tissues were excised and homogenized in PBS with protease inhibitor cocktail (Sigma-Aldrich, USA) and Triton X-100 (final concentration 1%). The samples were frozen at −70°C, thawed, and centrifuged at 10,000×g for 5 min to remove cellular debris. The tissue lysates were performed protein assay in the same way as for western blotting. 4.5 mg of proteins, collected from three samples per group, were used for Mouse cytokine array as the protocol provided by supplier (Proteome Profiler™, #ARY006, R\u0026D Systems, USA)."}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T16733","span":{"begin":252,"end":260},"obj":"http://purl.obolibrary.org/obo/GO_0007349"}],"text":"The tumor and spleen tissues were excised and homogenized in PBS with protease inhibitor cocktail (Sigma-Aldrich, USA) and Triton X-100 (final concentration 1%). The samples were frozen at −70°C, thawed, and centrifuged at 10,000×g for 5 min to remove cellular debris. The tissue lysates were performed protein assay in the same way as for western blotting. 4.5 mg of proteins, collected from three samples per group, were used for Mouse cytokine array as the protocol provided by supplier (Proteome Profiler™, #ARY006, R\u0026D Systems, USA)."}

    sentences

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    bionlp-st-ge-2016-test-ihmc

    {"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T16961","span":{"begin":70,"end":118},"obj":"Entity"},{"id":"T16960","span":{"begin":99,"end":104},"obj":"Protein"},{"id":"T16959","span":{"begin":123,"end":160},"obj":"Entity"},{"id":"T16958","span":{"begin":303,"end":316},"obj":"Protein"},{"id":"T16957","span":{"begin":368,"end":417},"obj":"Protein"},{"id":"T16956","span":{"begin":70,"end":78},"obj":"Protein"},{"id":"T16955","span":{"begin":432,"end":452},"obj":"Protein"}],"text":"The tumor and spleen tissues were excised and homogenized in PBS with protease inhibitor cocktail (Sigma-Aldrich, USA) and Triton X-100 (final concentration 1%). The samples were frozen at −70°C, thawed, and centrifuged at 10,000×g for 5 min to remove cellular debris. The tissue lysates were performed protein assay in the same way as for western blotting. 4.5 mg of proteins, collected from three samples per group, were used for Mouse cytokine array as the protocol provided by supplier (Proteome Profiler™, #ARY006, R\u0026D Systems, USA)."}

    bionlp-st-ge-2016-spacy-parsed

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    bionlp-st-ge-2016-test-tees

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