PMC:3342329 / 28554-29828 JSON TXT

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test3

{"project":"test3","denotations":[{"id":"T14067","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14066","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14065","span":{"begin":762,"end":765},"obj":"Protein"},{"id":"T14064","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14063","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14062","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

testone

{"project":"testone","denotations":[{"id":"T14061","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14060","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14059","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T14351","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14350","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14349","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T14338","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14337","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14336","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T14084","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14083","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14082","span":{"begin":762,"end":765},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T14342","span":{"begin":951,"end":960},"obj":"http://www.uniprot.org/uniprot/P42574"},{"id":"T14341","span":{"begin":770,"end":773},"obj":"http://www.uniprot.org/uniprot/P19838"},{"id":"T14340","span":{"begin":762,"end":765},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T14339","span":{"begin":762,"end":765},"obj":"http://www.uniprot.org/uniprot/Q04206"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T14070","span":{"begin":496,"end":500},"obj":"http://purl.obolibrary.org/obo/UBERON_0001913"},{"id":"T14069","span":{"begin":157,"end":163},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T14068","span":{"begin":6,"end":13},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T14085","span":{"begin":830,"end":832},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T14098","span":{"begin":830,"end":832},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T14097","span":{"begin":853,"end":863},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T14096","span":{"begin":734,"end":744},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T14095","span":{"begin":705,"end":715},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T14094","span":{"begin":1010,"end":1014},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14093","span":{"begin":853,"end":863},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14092","span":{"begin":734,"end":744},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14091","span":{"begin":705,"end":715},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T14090","span":{"begin":853,"end":863},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14089","span":{"begin":734,"end":744},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14088","span":{"begin":705,"end":715},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T14087","span":{"begin":649,"end":658},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T14086","span":{"begin":357,"end":365},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

sentences

{"project":"sentences","denotations":[{"id":"T14074","span":{"begin":421,"end":540},"obj":"Sentence"},{"id":"T14073","span":{"begin":153,"end":420},"obj":"Sentence"},{"id":"T14072","span":{"begin":0,"end":152},"obj":"Sentence"},{"id":"T14078","span":{"begin":1206,"end":1274},"obj":"Sentence"},{"id":"T14077","span":{"begin":1051,"end":1205},"obj":"Sentence"},{"id":"T14076","span":{"begin":614,"end":1050},"obj":"Sentence"},{"id":"T14075","span":{"begin":541,"end":613},"obj":"Sentence"},{"id":"T218","span":{"begin":0,"end":152},"obj":"Sentence"},{"id":"T219","span":{"begin":153,"end":215},"obj":"Sentence"},{"id":"T220","span":{"begin":216,"end":420},"obj":"Sentence"},{"id":"T221","span":{"begin":421,"end":540},"obj":"Sentence"},{"id":"T222","span":{"begin":541,"end":613},"obj":"Sentence"},{"id":"T223","span":{"begin":614,"end":1050},"obj":"Sentence"},{"id":"T224","span":{"begin":1051,"end":1205},"obj":"Sentence"},{"id":"T225","span":{"begin":1206,"end":1274},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

simple1

{"project":"simple1","denotations":[{"id":"T14101","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14100","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14099","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T14345","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14344","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14343","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T14405","span":{"begin":31,"end":98},"obj":"Negative_regulation"},{"id":"T14404","span":{"begin":322,"end":356},"obj":"Entity"},{"id":"T14403","span":{"begin":118,"end":129},"obj":"Protein"},{"id":"T14402","span":{"begin":951,"end":960},"obj":"Protein"},{"id":"T14401","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14400","span":{"begin":1010,"end":1014},"obj":"Entity"},{"id":"T14399","span":{"begin":541,"end":551},"obj":"Entity"},{"id":"T14398","span":{"begin":357,"end":401},"obj":"Entity"},{"id":"T14397","span":{"begin":473,"end":500},"obj":"Entity"},{"id":"T14396","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14395","span":{"begin":717,"end":744},"obj":"Protein"},{"id":"T14394","span":{"begin":1046,"end":1048},"obj":"Protein"},{"id":"T14393","span":{"begin":77,"end":97},"obj":"Entity"},{"id":"T14392","span":{"begin":912,"end":917},"obj":"Entity"},{"id":"T14391","span":{"begin":1206,"end":1229},"obj":"Protein"},{"id":"T14390","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T14389","span":{"begin":268,"end":297},"obj":"Entity"},{"id":"T14388","span":{"begin":697,"end":744},"obj":"Entity"},{"id":"T14387","span":{"begin":572,"end":576},"obj":"Entity"},{"id":"T14386","span":{"begin":36,"end":43},"obj":"Protein"},{"id":"T14385","span":{"begin":148,"end":151},"obj":"Entity"},{"id":"T14384","span":{"begin":444,"end":500},"obj":"Entity"},{"id":"T14383","span":{"begin":268,"end":297},"obj":"Entity"},{"id":"T14382","span":{"begin":889,"end":893},"obj":"Protein"},{"id":"T14381","span":{"begin":645,"end":658},"obj":"Entity"},{"id":"T14380","span":{"begin":762,"end":765},"obj":"Protein"},{"id":"T14379","span":{"begin":962,"end":971},"obj":"Protein"},{"id":"T14378","span":{"begin":842,"end":893},"obj":"Entity"},{"id":"T14377","span":{"begin":230,"end":249},"obj":"Protein"},{"id":"T14376","span":{"begin":1253,"end":1256},"obj":"Protein"},{"id":"T14375","span":{"begin":792,"end":797},"obj":"Entity"},{"id":"T14374","span":{"begin":1108,"end":1118},"obj":"Protein"},{"id":"T14373","span":{"begin":351,"end":355},"obj":"Entity"},{"id":"T14372","span":{"begin":697,"end":744},"obj":"Entity"},{"id":"T14371","span":{"begin":881,"end":884},"obj":"Protein"},{"id":"T14370","span":{"begin":565,"end":602},"obj":"Entity"},{"id":"T14369","span":{"begin":411,"end":413},"obj":"Protein"},{"id":"T14368","span":{"begin":973,"end":978},"obj":"Protein"}],"relations":[{"id":"R9971","pred":"themeOf","subj":"T14386","obj":"T14405"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T14359","span":{"begin":1096,"end":1159},"obj":"Protein"},{"id":"T14358","span":{"begin":983,"end":990},"obj":"Protein"},{"id":"T14357","span":{"begin":973,"end":978},"obj":"Protein"},{"id":"T14356","span":{"begin":962,"end":971},"obj":"Protein"},{"id":"T14355","span":{"begin":951,"end":960},"obj":"Protein"},{"id":"T14354","span":{"begin":889,"end":893},"obj":"Protein"},{"id":"T14353","span":{"begin":770,"end":773},"obj":"Protein"},{"id":"T14352","span":{"begin":762,"end":765},"obj":"Protein"}],"text":"Tumor tissues were homogenized with protein extraction solution (PRO-PREPTM, Intron Biotechnology, Seoul, Korea), and lysed by 60 min incubation on ice. The tissue lysate centrifuged at 15,000 rpm for 15 min at 4°C. Equal amounts of proteins (40 µg) were separated on a SDS/12%-polyacrylamide gel, and then transferred to a polyvinylidene difluoride (PVDF) membrane (GE Water and Process technologies, Trevose, PA, USA). Blots were blocked for 1 h at room temperature with 5% (w/v) non-fat dried milk in Tris-Buffered Saline Tween-20 [TBST: 10 mM Tris (pH 8.0) and 150 mM NaCl solution containing 0.05% Tween-20]. After a short washing in TBST, the membranes were immunoblotted with the following primary antibodies: mouse monoclonal antibodies directed against p65 and p50 (1∶500 dilutions; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal antibodies directed against Bax and PARP (1∶500 dilutions; Santa Cruz Biotechnology), and against caspase-3, caspase-9, Bcl-2 and c-IAP-1 (1∶1000 dilutions; Cell Signaling Technology, Beverly, MA). The blots were incubated with the respective horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (1∶4000 dilutions; Santa Cruz Biotechnology). Immunoreactive proteins were detected with the ECL detection system."}

PMC:3342329 / 28554-29828 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3342329 / 28554-29828 JSON TXT