PMC:3333881 / 8081-8841 JSONTXT

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    pmc-enju-pas

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interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

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interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    UBERON-AE

    {"project":"UBERON-AE","denotations":[{"id":"T5668","span":{"begin":248,"end":253},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    GO-BP

    {"project":"GO-BP","denotations":[{"id":"T5972","span":{"begin":0,"end":16},"obj":"http://purl.obolibrary.org/obo/GO_0016246"},{"id":"T5973","span":{"begin":426,"end":442},"obj":"http://purl.obolibrary.org/obo/GO_0016246"},{"id":"T5974","span":{"begin":646,"end":662},"obj":"http://purl.obolibrary.org/obo/GO_0016246"},{"id":"T5975","span":{"begin":133,"end":135},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    GO-MF

    {"project":"GO-MF","denotations":[{"id":"T5976","span":{"begin":133,"end":135},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    GO-CC

    {"project":"GO-CC","denotations":[{"id":"T5969","span":{"begin":32,"end":37},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5970","span":{"begin":170,"end":175},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5971","span":{"begin":375,"end":380},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    sentences

    {"project":"sentences","denotations":[{"id":"T5677","span":{"begin":0,"end":16},"obj":"Sentence"},{"id":"T5678","span":{"begin":17,"end":142},"obj":"Sentence"},{"id":"T5679","span":{"begin":143,"end":425},"obj":"Sentence"},{"id":"T5680","span":{"begin":426,"end":645},"obj":"Sentence"},{"id":"T5681","span":{"begin":646,"end":760},"obj":"Sentence"},{"id":"T50","span":{"begin":0,"end":16},"obj":"Sentence"},{"id":"T51","span":{"begin":17,"end":142},"obj":"Sentence"},{"id":"T52","span":{"begin":143,"end":425},"obj":"Sentence"},{"id":"T53","span":{"begin":426,"end":645},"obj":"Sentence"},{"id":"T54","span":{"begin":646,"end":760},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    events-check-again

    {"project":"events-check-again","denotations":[{"id":"T6014","span":{"begin":463,"end":478},"obj":"Protein"},{"id":"T6015","span":{"begin":506,"end":525},"obj":"Protein"},{"id":"T6016","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T6017","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T6018","span":{"begin":666,"end":669},"obj":"Protein"},{"id":"T6019","span":{"begin":674,"end":679},"obj":"Protein"},{"id":"T6020","span":{"begin":710,"end":719},"obj":"Protein"},{"id":"T6021","span":{"begin":724,"end":729},"obj":"Protein"}],"relations":[{"id":"R5176","pred":"themeOf","subj":"T6018","obj":"T6016"},{"id":"R5177","pred":"themeOf","subj":"T6019","obj":"T6017"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    bionlp-st-ge-2016-reference-tees

    {"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T6022","span":{"begin":86,"end":89},"obj":"Protein"},{"id":"T6023","span":{"begin":463,"end":478},"obj":"Protein"},{"id":"T6024","span":{"begin":480,"end":501},"obj":"Protein"},{"id":"T6025","span":{"begin":506,"end":515},"obj":"Protein"},{"id":"T6026","span":{"begin":666,"end":669},"obj":"Protein"},{"id":"T6027","span":{"begin":674,"end":679},"obj":"Protein"},{"id":"T6028","span":{"begin":699,"end":715},"obj":"Protein"},{"id":"T6029","span":{"begin":716,"end":719},"obj":"Protein"},{"id":"T6030","span":{"begin":724,"end":729},"obj":"Protein"},{"id":"T6031","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T6032","span":{"begin":650,"end":662},"obj":"Negative_regulation"}],"relations":[{"id":"R5178","pred":"themeOf","subj":"T6026","obj":"T6031"},{"id":"R5179","pred":"themeOf","subj":"T6027","obj":"T6032"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    test2

    {"project":"test2","denotations":[{"id":"T5661","span":{"begin":463,"end":478},"obj":"Protein"},{"id":"T5662","span":{"begin":506,"end":525},"obj":"Protein"},{"id":"T5663","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T5664","span":{"begin":666,"end":669},"obj":"Protein"},{"id":"T5665","span":{"begin":674,"end":679},"obj":"Protein"},{"id":"T5666","span":{"begin":710,"end":719},"obj":"Protein"},{"id":"T5667","span":{"begin":724,"end":729},"obj":"Protein"}],"relations":[{"id":"R4889","pred":"themeOf","subj":"T5664","obj":"T5663"},{"id":"R4890","pred":"themeOf","subj":"T5665","obj":"T5663"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    bionlp-st-ge-2016-reference

    {"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T5669","span":{"begin":463,"end":478},"obj":"Protein"},{"id":"T5670","span":{"begin":506,"end":525},"obj":"Protein"},{"id":"T5671","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T5672","span":{"begin":650,"end":662},"obj":"Negative_regulation"},{"id":"T5673","span":{"begin":666,"end":669},"obj":"Protein"},{"id":"T5674","span":{"begin":674,"end":679},"obj":"Protein"},{"id":"T5675","span":{"begin":710,"end":719},"obj":"Protein"},{"id":"T5676","span":{"begin":724,"end":729},"obj":"Protein"}],"relations":[{"id":"R4891","pred":"themeOf","subj":"T5673","obj":"T5671"},{"id":"R4892","pred":"themeOf","subj":"T5674","obj":"T5672"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5812","span":{"begin":469,"end":472},"obj":"P04605"},{"id":"T5813","span":{"begin":469,"end":472},"obj":"P04608"},{"id":"T5814","span":{"begin":469,"end":472},"obj":"P04610"},{"id":"T5815","span":{"begin":469,"end":472},"obj":"Q8UMQ1"},{"id":"T5816","span":{"begin":512,"end":515},"obj":"P04610"},{"id":"T5817","span":{"begin":512,"end":515},"obj":"P04608"},{"id":"T5818","span":{"begin":512,"end":515},"obj":"Q8UMQ1"},{"id":"T5819","span":{"begin":512,"end":515},"obj":"P04605"},{"id":"T5820","span":{"begin":666,"end":669},"obj":"Q04206"},{"id":"T5821","span":{"begin":666,"end":669},"obj":"P21579"},{"id":"T5822","span":{"begin":716,"end":719},"obj":"Q04206"},{"id":"T5823","span":{"begin":716,"end":719},"obj":"P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"RNA interference\nJurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA). Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium. RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU. RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA)."}