PMC:3333881 / 5825-14980 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
22187158-17942396-77584437	252-254	17942396	denotes	50
22187158-14532125-77584438	845-847	14532125	denotes	52
22187158-19782003-77584439	1062-1064	19782003	denotes	53
22187158-17942396-77584440	3490-3492	17942396	denotes	50
22187158-8537390-77584441	3642-3644	8537390	denotes	54
22187158-15613239-77584442	5057-5059	15613239	denotes	55
22187158-17942396-77584443	5308-5310	17942396	denotes	50
22187158-17942396-77584444	5541-5543	17942396	denotes	50

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T4413	23-31	NN	denotes	Plasmids
T4414	32-35	DT	denotes	The
T4415	36-44	NN	denotes	plasmids
T4416	45-61	NN	denotes	pcDNA-3xHA-IκB-α
T4417	61-62	-COMMA-	denotes	,
T4418	63-78	NN	denotes	p3xFLAG-CMV-Tat
T4419	78-79	-COMMA-	denotes	,
T4420	80-95	NN	denotes	p3xFLAG-CMV-Tat
T4421	96-97	NN	denotes	C
T4422	97-98	-LRB-	denotes	(
T4423	98-106	CD	denotes	22,25,27
T4424	106-107	-RRB-	denotes	)
T4425	107-108	NN	denotes	A
T4426	108-109	-COMMA-	denotes	,
T4427	110-125	NN	denotes	p3xFLAG-CMV-Tat
T4428	126-127	NN	denotes	R
T4429	127-128	-LRB-	denotes	(
T4430	128-145	CD	denotes	49,52,53,55,56,57
T4431	145-146	-RRB-	denotes	)
T4432	146-147	NN	denotes	A
T4433	147-148	-COMMA-	denotes	,
T4434	149-160	NN	denotes	pGEX-2T-Tat
T4435	160-161	-COMMA-	denotes	,
T4436	162-173	NN	denotes	pGEX-2T-Tat
T4437	174-175	NN	denotes	C
T4438	175-176	-LRB-	denotes	(
T4439	176-184	CD	denotes	22,25,27
T4440	184-185	-RRB-	denotes	)
T4441	185-186	NN	denotes	A
T4442	187-190	CC	denotes	and
T4443	191-202	NN	denotes	pGEX-2T-Tat
T4444	203-204	NN	denotes	R
T4445	204-205	-LRB-	denotes	(
T4446	205-222	CD	denotes	49,52,53,55,56,57
T4447	222-223	-RRB-	denotes	)
T4448	223-224	NN	denotes	A
T4449	225-229	VB	denotes	were
T4450	230-240	RB	denotes	previously
T4451	241-250	VB	denotes	described
T4452	251-252	-LRB-	denotes	(
T4453	252-254	CD	denotes	50
T4454	254-255	-RRB-	denotes	)
T4455	257-260	DT	denotes	The
T4456	261-269	NN	denotes	plasmids
T4457	270-285	NN	denotes	pNL4-3.Luc.R-E-
T4458	286-289	CC	denotes	and
T4459	290-299	NN	denotes	pHXB2-env
T4460	300-304	VB	denotes	were
T4461	305-313	VB	denotes	obtained
T4462	314-318	IN	denotes	from
T4463	319-322	DT	denotes	the
T4464	323-327	NNP	denotes	AIDS
T4465	328-336	NNP	denotes	Research
T4466	337-338	CC	denotes	&
T4467	339-348	NNP	denotes	Reference
T4468	349-356	NNP	denotes	Reagent
T4469	357-364	NNP	denotes	Program
T4470	364-365	-COMMA-	denotes	,
T4471	366-374	NNP	denotes	Division
T4472	375-377	IN	denotes	of
T4473	378-382	NNP	denotes	AIDS
T4474	382-383	-COMMA-	denotes	,
T4475	384-389	NNP	denotes	NIAID
T4476	389-390	-COMMA-	denotes	,
T4477	391-394	NNP	denotes	NIH
T4478	394-395	-COMMA-	denotes	,
T4479	396-399	NNP	denotes	USA
T4480	399-400	-COLON-	denotes	;
T4481	401-407	NN	denotes	pκBluc
T4482	408-411	CC	denotes	and
T4483	412-421	NN	denotes	pSV-β-Gal
T4484	422-426	VB	denotes	were
T4485	427-436	VB	denotes	purchased
T4486	437-441	IN	denotes	from
T4487	442-449	NNP	denotes	Promega
T4488	450-451	-LRB-	denotes	(
T4489	451-458	NNP	denotes	Madison
T4490	458-459	-COMMA-	denotes	,
T4491	460-462	NNP	denotes	WI
T4492	462-463	-COMMA-	denotes	,
T4493	464-467	NNP	denotes	USA
T4494	467-468	-RRB-	denotes	)
T4495	470-473	DT	denotes	The
T4496	474-482	NN	denotes	plasmids
T4497	483-499	NN	denotes	pRc/CMV-3xHA-p65
T4498	499-500	-COMMA-	denotes	,
T4499	501-519	NN	denotes	pRc/CMV-3xHA-p65ΔC
T4500	519-520	-LRB-	denotes	(
T4501	520-525	CD	denotes	1–318
T4502	525-526	-RRB-	denotes	)
T4503	526-527	-COMMA-	denotes	,
T4504	528-546	NN	denotes	pRc/CMV-3xHA-p65ΔN
T4505	546-547	-LRB-	denotes	(
T4506	547-554	CD	denotes	122–551
T4507	554-555	-RRB-	denotes	)
T4508	555-556	-COMMA-	denotes	,
T4509	557-572	NN	denotes	p3xFLAG-CMV-Tat
T4510	573-574	NN	denotes	T
T4511	574-575	-COMMA-	denotes	,
T4512	575-576	NN	denotes	N
T4513	576-577	-LRB-	denotes	(
T4514	577-582	CD	denotes	23,24
T4515	582-583	-RRB-	denotes	)
T4516	583-584	NN	denotes	A
T4517	584-585	-COMMA-	denotes	,
T4518	586-601	NN	denotes	p3xFLAG-CMV-Tat
T4519	602-603	NN	denotes	K
T4520	603-604	-LRB-	denotes	(
T4521	604-609	CD	denotes	50,51
T4522	609-610	-RRB-	denotes	)
T4523	610-611	NN	denotes	A
T4524	611-612	-COMMA-	denotes	,
T4525	613-624	NN	denotes	pGEX-2T-Tat
T4526	625-626	NN	denotes	T
T4527	626-627	-COMMA-	denotes	,
T4528	627-628	NN	denotes	N
T4529	628-629	-LRB-	denotes	(
T4530	629-634	CD	denotes	23,24
T4531	634-635	-RRB-	denotes	)
T4532	635-636	NN	denotes	A
T4533	636-637	-COMMA-	denotes	,
T4534	638-649	NN	denotes	pGEX-2T-Tat
T4535	650-651	NN	denotes	K
T4536	651-652	-LRB-	denotes	(
T4537	652-657	CD	denotes	50,51
T4538	657-658	-RRB-	denotes	)
T4539	658-659	NN	denotes	A
T4540	660-663	CC	denotes	and
T4541	664-684	NN	denotes	pNL4-3.FLAG-Tat.R-E-
T4542	685-689	VB	denotes	were
T4543	690-699	VB	denotes	generated
T4544	700-702	IN	denotes	as
T4545	703-712	VB	denotes	described
T4546	713-715	IN	denotes	in
T4547	716-729	NNP	denotes	Supplementary
T4548	730-734	NNP	denotes	Data
T4915	737-742	NN	denotes	Cells
T4916	742-743	-COMMA-	denotes	,
T4917	744-756	NN	denotes	transfection
T4918	756-757	-COMMA-	denotes	,
T4919	758-768	NN	denotes	treatments
T4920	769-772	CC	denotes	and
T4921	773-783	NN	denotes	luciferase
T4922	784-789	NN	denotes	assay
T4923	790-794	NN	denotes	HeLa
T4924	794-795	-COMMA-	denotes	,
T4925	796-808	NN	denotes	p50−/−p65−/−
T4926	809-814	NN	denotes	mouse
T4927	815-824	JJ	denotes	embryonic
T4928	825-836	NN	denotes	fibroblasts
T4929	837-838	-LRB-	denotes	(
T4930	838-842	NN	denotes	MEFs
T4931	842-843	-RRB-	denotes	)
T4932	844-845	-LRB-	denotes	(
T4933	845-847	CD	denotes	52
T4934	847-848	-RRB-	denotes	)
T4935	849-852	CC	denotes	and
T4936	853-857	NN	denotes	293T
T4937	858-863	NN	denotes	cells
T4938	864-868	VB	denotes	were
T4939	869-877	VB	denotes	cultured
T4940	878-880	IN	denotes	in
T4941	881-889	NNP	denotes	Dulbecco
T4942	889-891	POS	denotes	's
T4943	892-900	VB	denotes	modified
T4944	901-906	NNP	denotes	Eagle
T4945	906-908	POS	denotes	's
T4946	909-915	NN	denotes	medium
T4947	915-916	-COLON-	denotes	;
T4948	917-923	NN	denotes	Jurkat
T4949	923-924	-COMMA-	denotes	,
T4950	925-929	NN	denotes	U937
T4951	930-935	NN	denotes	cells
T4952	936-939	CC	denotes	and
T4953	940-945	JJ	denotes	human
T4954	946-956	JJ	denotes	peripheral
T4955	957-962	NN	denotes	blood
T4956	963-974	JJ	denotes	mononuclear
T4957	975-980	NN	denotes	cells
T4958	981-982	-LRB-	denotes	(
T4959	982-987	NN	denotes	PBMCs
T4960	987-988	-RRB-	denotes	)
T4961	989-993	VB	denotes	were
T4962	994-1002	VB	denotes	cultured
T4963	1003-1005	IN	denotes	in
T4964	1006-1010	NN	denotes	RPMI
T4965	1011-1015	CD	denotes	1640
T4966	1017-1022	NN	denotes	PBMCs
T4967	1023-1027	VB	denotes	were
T4968	1028-1036	VB	denotes	isolated
T4969	1037-1039	IN	denotes	as
T4970	1040-1050	RB	denotes	previously
T4971	1051-1060	VB	denotes	described
T4972	1061-1062	-LRB-	denotes	(
T4973	1062-1064	CD	denotes	53
T4974	1064-1065	-RRB-	denotes	)
T4975	1067-1072	NN	denotes	Media
T4976	1073-1077	VB	denotes	were
T4977	1078-1090	VB	denotes	supplemented
T4978	1091-1095	IN	denotes	with
T4979	1096-1098	CD	denotes	10
T4980	1098-1099	NN	denotes	%
T4981	1100-1116	JJ	denotes	heat-inactivated
T4982	1117-1122	JJ	denotes	fetal
T4983	1123-1127	NN	denotes	calf
T4984	1128-1133	NN	denotes	serum
T4985	1134-1137	CC	denotes	and
T4986	1138-1142	NN	denotes	2 mM
T4987	1143-1154	NN	denotes	l-glutamine
T4988	1155-1156	-LRB-	denotes	(
T4989	1156-1161	NNP	denotes	Lonza
T4990	1162-1169	NNP	denotes	Cologne
T4991	1170-1172	NNP	denotes	AG
T4992	1172-1173	-COMMA-	denotes	,
T4993	1174-1181	NNP	denotes	Germany
T4994	1181-1182	-RRB-	denotes	)
T4995	1184-1188	NN	denotes	HeLa
T4996	1188-1189	-COMMA-	denotes	,
T4997	1190-1202	NN	denotes	p50−/−p65−/−
T4998	1203-1207	NN	denotes	MEFs
T4999	1208-1211	CC	denotes	and
T5000	1212-1216	NN	denotes	293T
T5001	1217-1221	VB	denotes	were
T5002	1222-1233	VB	denotes	transfected
T5003	1234-1238	IN	denotes	with
T5004	1239-1242	NN	denotes	DNA
T5005	1243-1245	IN	denotes	by
T5006	1246-1251	VB	denotes	using
T5007	1252-1258	NN	denotes	FuGENE
T5008	1259-1261	NN	denotes	HD
T5009	1262-1263	-LRB-	denotes	(
T5010	1263-1268	NNP	denotes	Roche
T5011	1269-1279	NNP	denotes	Diagnostic
T5012	1280-1284	NNP	denotes	GmbH
T5013	1284-1285	-COMMA-	denotes	,
T5014	1286-1294	NNP	denotes	Mannheim
T5015	1294-1295	-COMMA-	denotes	,
T5016	1296-1303	NNP	denotes	Germany
T5017	1303-1304	-RRB-	denotes	)
T5018	1304-1305	-COMMA-	denotes	,
T5019	1306-1315	VB	denotes	according
T5020	1316-1318	TO	denotes	to
T5021	1319-1322	DT	denotes	the
T5022	1323-1335	NN	denotes	manufacturer
T5023	1335-1337	POS	denotes	's
T5024	1338-1346	NN	denotes	protocol
T5025	1346-1347	-COLON-	denotes	;
T5026	1348-1353	JJ	denotes	total
T5027	1354-1357	NN	denotes	DNA
T5028	1358-1365	NN	denotes	amounts
T5029	1366-1370	VB	denotes	were
T5030	1371-1380	VB	denotes	equalized
T5031	1381-1383	IN	denotes	by
T5032	1384-1396	NN	denotes	transfection
T5033	1397-1399	IN	denotes	of
T5034	1400-1407	NN	denotes	pRc/CMV
T5035	1408-1413	NN	denotes	empty
T5036	1414-1420	NN	denotes	vector
T5037	1421-1422	-LRB-	denotes	(
T5038	1422-1432	NN	denotes	Invitrogen
T5039	1432-1433	-COMMA-	denotes	,
T5040	1434-1442	NNP	denotes	Carlsbad
T5041	1442-1443	-COMMA-	denotes	,
T5042	1444-1446	NNP	denotes	CA
T5043	1446-1447	-COMMA-	denotes	,
T5044	1448-1451	NNP	denotes	USA
T5045	1451-1452	-RRB-	denotes	)
T5046	1454-1457	IN	denotes	For
T5047	1458-1475	NN	denotes	pulse-stimulation
T5048	1475-1476	-COMMA-	denotes	,
T5049	1477-1481	NN	denotes	HeLa
T5050	1482-1487	NN	denotes	cells
T5051	1488-1492	VB	denotes	were
T5052	1493-1500	VB	denotes	treated
T5053	1501-1505	IN	denotes	with
T5054	1506-1513	NN	denotes	phorbol
T5055	1514-1526	NN	denotes	12-myristate
T5056	1527-1537	NN	denotes	13-acetate
T5057	1538-1539	-LRB-	denotes	(
T5058	1539-1542	NN	denotes	PMA
T5059	1542-1543	-COLON-	denotes	;
T5060	1544-1557	JJ	denotes	Sigma-Aldrich
T5061	1557-1558	-COMMA-	denotes	,
T5062	1559-1561	NNP	denotes	St
T5063	1562-1567	NNP	denotes	Louis
T5064	1567-1568	-COMMA-	denotes	,
T5065	1569-1571	NN	denotes	MO
T5066	1571-1572	-COMMA-	denotes	,
T5067	1573-1576	NNP	denotes	USA
T5068	1576-1577	-RRB-	denotes	)
T5069	1578-1579	-LRB-	denotes	(
T5070	1579-1587	NN	denotes	20 ng/ml
T5071	1587-1588	-RRB-	denotes	)
T5072	1589-1592	IN	denotes	for
T5073	1593-1598	NN	denotes	5 min
T5074	1598-1599	-COMMA-	denotes	,
T5075	1600-1602	CC	denotes	or
T5076	1603-1608	NN	denotes	tumor
T5077	1609-1617	NN	denotes	necrosis
T5078	1618-1626	NN	denotes	factor-α
T5079	1627-1628	-LRB-	denotes	(
T5080	1628-1633	NN	denotes	TNF-α
T5081	1633-1634	-COLON-	denotes	;
T5082	1635-1648	JJ	denotes	Sigma-Aldrich
T5083	1648-1649	-RRB-	denotes	)
T5084	1650-1651	-LRB-	denotes	(
T5085	1651-1659	NN	denotes	20 ng/ml
T5086	1659-1660	-RRB-	denotes	)
T5087	1661-1664	IN	denotes	for
T5088	1665-1671	NN	denotes	30 min
T5089	1671-1672	-COMMA-	denotes	,
T5090	1673-1679	VB	denotes	washed
T5091	1680-1685	RB	denotes	twice
T5092	1686-1688	IN	denotes	in
T5093	1689-1697	JJ	denotes	complete
T5094	1698-1705	NN	denotes	culture
T5095	1706-1712	NN	denotes	medium
T5096	1713-1716	CC	denotes	and
T5097	1717-1721	RB	denotes	then
T5098	1722-1730	VB	denotes	returned
T5099	1731-1733	TO	denotes	to
T5100	1734-1741	NN	denotes	culture
T5101	1743-1746	IN	denotes	For
T5102	1747-1757	NN	denotes	luciferase
T5103	1758-1764	NN	denotes	assays
T5104	1764-1765	-COMMA-	denotes	,
T5105	1766-1775	NN	denotes	pSV-β-Gal
T5106	1776-1779	VB	denotes	was
T5107	1780-1794	VB	denotes	co-transfected
T5108	1795-1799	IN	denotes	with
T5109	1800-1806	NN	denotes	pκBluc
T5110	1807-1809	TO	denotes	to
T5111	1810-1817	VB	denotes	monitor
T5112	1818-1821	DT	denotes	the
T5113	1822-1834	NN	denotes	transfection
T5114	1835-1845	NN	denotes	efficiency
T5115	1847-1863	JJ	denotes	Forty-eight-hour
T5116	1864-1881	JJ	denotes	post-transfection
T5117	1881-1882	-COMMA-	denotes	,
T5118	1883-1888	NN	denotes	cells
T5119	1889-1893	VB	denotes	were
T5120	1894-1899	VB	denotes	lysed
T5121	1900-1902	IN	denotes	in
T5122	1903-1908	NN	denotes	lysis
T5123	1909-1915	NN	denotes	buffer
T5124	1916-1918	IN	denotes	of
T5125	1919-1923	NNP	denotes	Dual
T5126	1924-1929	NNP	denotes	Light
T5127	1930-1940	NNP	denotes	Luciferase
T5128	1941-1947	NNP	denotes	System
T5129	1948-1949	-LRB-	denotes	(
T5130	1949-1955	NNP	denotes	Tropix
T5131	1955-1956	-COMMA-	denotes	,
T5132	1957-1964	NNP	denotes	Bedford
T5133	1964-1965	-COMMA-	denotes	,
T5134	1966-1968	NNP	denotes	MA
T5135	1968-1969	-COMMA-	denotes	,
T5136	1970-1973	NNP	denotes	USA
T5137	1973-1974	-RRB-	denotes	)
T5138	1975-1978	CC	denotes	and
T5139	1979-1982	DT	denotes	the
T5140	1983-1993	NN	denotes	luciferase
T5141	1994-1997	CC	denotes	and
T5142	1998-2013	NN	denotes	β-galactosidase
T5143	2014-2024	NN	denotes	activities
T5144	2025-2029	VB	denotes	were
T5145	2030-2039	VB	denotes	evaluated
T5146	2040-2042	IN	denotes	by
T5147	2043-2048	VB	denotes	using
T5148	2049-2053	NNP	denotes	Dual
T5149	2054-2059	NNP	denotes	Light
T5150	2060-2070	NNP	denotes	Luciferase
T5151	2071-2077	NNP	denotes	System
T5152	2078-2079	-LRB-	denotes	(
T5153	2079-2085	NN	denotes	Tropix
T5154	2085-2086	-RRB-	denotes	)
T5155	2087-2089	IN	denotes	in
T5156	2090-2091	DT	denotes	a
T5157	2092-2106	NN	denotes	bioluminometer
T5158	2107-2108	-LRB-	denotes	(
T5159	2108-2114	NNP	denotes	Turner
T5160	2115-2124	NNP	denotes	Biosystem
T5161	2124-2125	-COMMA-	denotes	,
T5162	2126-2135	NNP	denotes	Sunnyvale
T5163	2135-2136	-COMMA-	denotes	,
T5164	2137-2139	NNP	denotes	CA
T5165	2139-2140	-COMMA-	denotes	,
T5166	2141-2144	NNP	denotes	USA
T5167	2144-2145	-RRB-	denotes	)
T5168	2147-2150	DT	denotes	The
T5169	2151-2156	NN	denotes	ratio
T5170	2157-2159	IN	denotes	of
T5171	2160-2167	NN	denotes	firefly
T5172	2168-2178	NN	denotes	luciferase
T5173	2179-2187	NN	denotes	activity
T5174	2188-2190	TO	denotes	to
T5175	2191-2206	NN	denotes	β-galactosidase
T5176	2207-2215	NN	denotes	activity
T5177	2216-2219	VB	denotes	was
T5178	2220-2229	VB	denotes	expressed
T5179	2230-2232	IN	denotes	as
T5180	2233-2241	JJ	denotes	relative
T5181	2242-2247	JJ	denotes	light
T5182	2248-2253	NN	denotes	units
T5682	2256-2259	NN	denotes	RNA
T5683	2260-2272	NN	denotes	interference
T5684	2273-2279	NN	denotes	Jurkat
T5685	2280-2282	CC	denotes	or
T5686	2283-2287	NN	denotes	U937
T5687	2288-2293	NN	denotes	cells
T5688	2294-2298	VB	denotes	were
T5689	2299-2310	VB	denotes	transfected
T5690	2311-2313	IN	denotes	by
T5691	2314-2329	NN	denotes	electroporation
T5692	2330-2335	VB	denotes	using
T5693	2336-2337	DT	denotes	a
T5694	2338-2345	JJ	denotes	Bio-Rad
T5695	2346-2355	NN	denotes	apparatus
T5696	2356-2357	-LRB-	denotes	(
T5697	2357-2364	NNP	denotes	Bio-Rad
T5698	2365-2377	NNP	denotes	Laboratories
T5699	2377-2378	-COMMA-	denotes	,
T5700	2379-2387	NNP	denotes	Hercules
T5701	2387-2388	-COMMA-	denotes	,
T5702	2389-2391	NNP	denotes	CA
T5703	2391-2392	-COMMA-	denotes	,
T5704	2393-2396	NNP	denotes	USA
T5705	2396-2397	-RRB-	denotes	)
T5706	2399-2406	RB	denotes	Briefly
T5707	2406-2407	-COMMA-	denotes	,
T5708	2408-2416	NN	denotes	aliquots
T5709	2417-2418	-LRB-	denotes	(
T5710	2418-2425	CD	denotes	5 × 106
T5711	2426-2431	NN	denotes	cells
T5712	2431-2432	-RRB-	denotes	)
T5713	2433-2437	VB	denotes	were
T5714	2438-2447	VB	denotes	suspended
T5715	2448-2450	IN	denotes	in
T5716	2451-2457	NN	denotes	0.3 ml
T5717	2458-2460	IN	denotes	of
T5718	2461-2465	NN	denotes	RPMI
T5719	2466-2470	CD	denotes	1640
T5720	2471-2483	VB	denotes	supplemented
T5721	2484-2488	IN	denotes	with
T5722	2489-2491	CD	denotes	20
T5723	2491-2492	NN	denotes	%
T5724	2493-2498	JJ	denotes	fetal
T5725	2499-2503	NN	denotes	calf
T5726	2504-2509	NN	denotes	serum
T5727	2510-2513	CC	denotes	and
T5728	2514-2523	VB	denotes	subjected
T5729	2524-2526	TO	denotes	to
T5730	2527-2528	DT	denotes	a
T5731	2529-2535	JJ	denotes	double
T5732	2536-2546	JJ	denotes	electrical
T5733	2547-2552	NN	denotes	pulse
T5734	2553-2554	-LRB-	denotes	(
T5735	2554-2560	NN	denotes	0.22 V
T5736	2560-2561	-COMMA-	denotes	,
T5737	2562-2568	NN	denotes	960 µF
T5738	2568-2569	-RRB-	denotes	)
T5739	2570-2572	IN	denotes	in
T5740	2573-2576	DT	denotes	the
T5741	2577-2585	NN	denotes	presence
T5742	2586-2588	IN	denotes	of
T5743	2589-2597	VB	denotes	annealed
T5744	2598-2603	NN	denotes	siRNA
T5745	2604-2605	-LRB-	denotes	(
T5746	2605-2613	NN	denotes	200 pmol
T5747	2613-2614	-RRB-	denotes	)
T5748	2614-2615	-COLON-	denotes	;
T5749	2616-2630	VB	denotes	electroporated
T5750	2631-2636	NN	denotes	cells
T5751	2637-2641	VB	denotes	were
T5752	2642-2648	VB	denotes	washed
T5753	2649-2652	CC	denotes	and
T5754	2653-2661	VB	denotes	cultured
T5755	2662-2664	IN	denotes	in
T5756	2665-2673	JJ	denotes	complete
T5757	2674-2680	NN	denotes	medium
T5758	2682-2685	NN	denotes	RNA
T5759	2686-2698	NN	denotes	interference
T5760	2699-2702	VB	denotes	was
T5761	2703-2712	VB	denotes	performed
T5762	2713-2717	IN	denotes	with
T5763	2717-2718	-COLON-	denotes	:
T5764	2719-2724	NN	denotes	siRNA
T5765	2725-2728	NN	denotes	Tat
T5766	2729-2734	NN	denotes	sense
T5767	2734-2735	-COMMA-	denotes	,
T5768	2736-2757	NN	denotes	CUGCUUGUACCAAUUGCUAUU
T5769	2758-2761	CC	denotes	and
T5770	2762-2767	NN	denotes	siRNA
T5771	2768-2771	NN	denotes	Tat
T5772	2772-2781	JJ	denotes	antisense
T5773	2781-2782	-COMMA-	denotes	,
T5774	2783-2804	NNP	denotes	UAGCAAUUGGUACAAGCAGUU
T5775	2804-2805	-COLON-	denotes	;
T5776	2806-2811	NN	denotes	siRNA
T5777	2812-2819	NN	denotes	control
T5778	2820-2825	NN	denotes	sense
T5779	2825-2826	-COMMA-	denotes	,
T5780	2827-2839	NN	denotes	CUGCUUGUCACA
T5781	2840-2849	NN	denotes	AUUGCUAUU
T5782	2850-2853	CC	denotes	and
T5783	2854-2859	NN	denotes	siRNA
T5784	2860-2867	NN	denotes	control
T5785	2868-2877	JJ	denotes	antisense
T5786	2877-2878	-COMMA-	denotes	,
T5787	2879-2900	NNP	denotes	UAGCAAUUGUGACAAGCAGUU
T5788	2902-2905	NN	denotes	RNA
T5789	2906-2918	NN	denotes	interference
T5790	2919-2921	IN	denotes	of
T5791	2922-2925	NN	denotes	p65
T5792	2926-2929	CC	denotes	and
T5793	2930-2935	NN	denotes	IκB-α
T5794	2936-2939	VB	denotes	was
T5795	2940-2949	VB	denotes	performed
T5796	2950-2954	IN	denotes	with
T5797	2955-2960	NN	denotes	SMART
T5798	2961-2965	NN	denotes	pool
T5799	2966-2971	NN	denotes	siRNA
T5800	2972-2975	NN	denotes	p65
T5801	2976-2979	CC	denotes	and
T5802	2980-2985	NN	denotes	IκB-α
T5803	2986-2987	-LRB-	denotes	(
T5804	2987-2996	NNP	denotes	Dharmacon
T5805	2996-2997	-COMMA-	denotes	,
T5806	2998-3005	NNP	denotes	Chicago
T5807	3005-3006	-COMMA-	denotes	,
T5808	3007-3009	NNP	denotes	IL
T5809	3009-3010	-COMMA-	denotes	,
T5810	3011-3014	NNP	denotes	USA
T5811	3014-3015	-RRB-	denotes	)
T6049	3018-3029	VB	denotes	Pseudotyped
T6050	3030-3037	NN	denotes	virions
T6051	3038-3041	CC	denotes	and
T6052	3042-3048	JJ	denotes	single
T6053	3049-3054	NN	denotes	round
T6054	3055-3064	NN	denotes	infection
T6055	3065-3069	NN	denotes	293T
T6056	3070-3075	NN	denotes	cells
T6057	3076-3077	-LRB-	denotes	(
T6058	3077-3084	CD	denotes	1 × 107
T6059	3084-3085	-RRB-	denotes	)
T6060	3086-3090	VB	denotes	were
T6061	3091-3102	VB	denotes	transfected
T6062	3103-3107	IN	denotes	with
T6063	3108-3123	NN	denotes	pNL4-3.Luc.R-E-
T6064	3124-3126	CC	denotes	or
T6065	3127-3147	NN	denotes	pNL4-3.FLAG-Tat.R-E-
T6066	3148-3149	-LRB-	denotes	(
T6067	3149-3154	NN	denotes	10 µg
T6068	3154-3155	-RRB-	denotes	)
T6069	3156-3164	RB	denotes	together
T6070	3165-3169	IN	denotes	with
T6071	3170-3175	NN	denotes	pHXB2
T6072	3176-3179	NN	denotes	Env
T6073	3180-3181	-LRB-	denotes	(
T6074	3181-3186	NN	denotes	10 µg
T6075	3186-3187	-RRB-	denotes	)
T6076	3187-3188	-COMMA-	denotes	,
T6078	3193-3197	JJ	denotes	48-h
T6079	3198-3215	JJ	denotes	post-transfection
T6080	3216-3220	NN	denotes	cell
T6081	3221-3232	NN	denotes	supernatant
T6082	3233-3236	VB	denotes	was
T6083	3237-3246	VB	denotes	collected
T6084	3248-3261	JJ	denotes	Enzyme-linked
T6085	3262-3275	JJ	denotes	immunosorbent
T6086	3276-3281	NN	denotes	assay
T6087	3282-3283	-LRB-	denotes	(
T6088	3283-3288	NN	denotes	ELISA
T6089	3288-3289	-RRB-	denotes	)
T6090	3290-3295	VB	denotes	using
T6091	3296-3304	JJ	denotes	anti-p24
T6092	3305-3313	NN	denotes	antibody
T6093	3314-3322	VB	denotes	measured
T6094	3323-3329	NN	denotes	virion
T6095	3330-3343	NN	denotes	concentration
T6096	3345-3350	NN	denotes	PBMCs
T6097	3350-3351	-COMMA-	denotes	,
T6098	3352-3358	NN	denotes	Jurkat
T6099	3359-3361	CC	denotes	or
T6100	3362-3366	NN	denotes	U937
T6101	3367-3372	NN	denotes	cells
T6102	3373-3374	-LRB-	denotes	(
T6103	3374-3381	CD	denotes	5 × 107
T6104	3381-3382	-RRB-	denotes	)
T6105	3383-3387	VB	denotes	were
T6106	3388-3396	VB	denotes	infected
T6107	3397-3401	IN	denotes	with
T6108	3402-3406	NN	denotes	HXB2
T6109	3407-3422	JJ	denotes	Env-pseudotyped
T6110	3423-3430	NN	denotes	virions
T6111	3431-3432	-LRB-	denotes	(
T6112	3432-3438	CD	denotes	500 ng
T6113	3439-3441	IN	denotes	of
T6114	3442-3445	NN	denotes	p24
T6115	3445-3446	-RRB-	denotes	)
T6116	3447-3449	IN	denotes	by
T6117	3450-3463	NN	denotes	spinoculation
T6118	3463-3464	-COMMA-	denotes	,
T6119	3465-3467	IN	denotes	as
T6120	3468-3478	RB	denotes	previously
T6121	3479-3488	VB	denotes	described
T6122	3489-3490	-LRB-	denotes	(
T6123	3490-3492	CD	denotes	50
T6124	3492-3493	-RRB-	denotes	)
T6718	5108-5110	FW	denotes	In
T6719	5111-5116	FW	denotes	vitro
T6720	5117-5128	NN	denotes	translation
T6721	5129-5137	NN	denotes	HA-IκB-α
T6722	5137-5138	-COMMA-	denotes	,
T6723	5139-5142	NN	denotes	p65
T6724	5143-5146	CC	denotes	and
T6725	5147-5150	NN	denotes	Tat
T6726	5151-5155	VB	denotes	were
T6727	5156-5165	VB	denotes	expressed
T6728	5166-5171	IN	denotes	under
T6729	5172-5175	DT	denotes	the
T6730	5176-5178	NN	denotes	T7
T6731	5179-5187	NN	denotes	promoter
T6732	5188-5191	CC	denotes	and
T6733	5192-5194	FW	denotes	in
T6734	5195-5200	FW	denotes	vitro
T6735	5201-5211	VB	denotes	translated
T6736	5212-5217	VB	denotes	using
T6737	5218-5221	DT	denotes	the
T6738	5222-5225	NN	denotes	TnT
T6739	5226-5231	JJ	denotes	quick
T6740	5232-5239	VB	denotes	coupled
T6741	5240-5265	NN	denotes	transcription/translation
T6742	5266-5272	NN	denotes	system
T6743	5273-5274	-LRB-	denotes	(
T6744	5274-5281	NN	denotes	Promega
T6745	5281-5282	-RRB-	denotes	)
T6746	5282-5283	-COMMA-	denotes	,
T6747	5284-5286	IN	denotes	as
T6748	5287-5297	RB	denotes	previously
T6749	5298-5306	VB	denotes	reported
T6750	5307-5308	-LRB-	denotes	(
T6751	5308-5310	CD	denotes	50
T6752	5310-5311	-RRB-	denotes	)
T6753	5313-5320	NN	denotes	Details
T6754	5321-5324	VB	denotes	are
T6755	5325-5334	VB	denotes	described
T6756	5335-5337	IN	denotes	in
T6757	5338-5351	NNP	denotes	Supplementary
T6758	5352-5356	NNP	denotes	Data
T6882	5359-5378	NN	denotes	Immunoprecipitation
T6883	5379-5384	NN	denotes	assay
T6884	5385-5388	CC	denotes	and
T6885	5389-5397	NN	denotes	GST-pull
T6886	5398-5402	RP	denotes	down
T6887	5403-5422	NN	denotes	Immunoprecipitation
T6888	5422-5423	-COMMA-	denotes	,
T6889	5424-5432	NNP	denotes	GST-pull
T6890	5433-5437	IN	denotes	down
T6891	5437-5438	-COMMA-	denotes	,
T6892	5439-5442	CC	denotes	and
T6893	5443-5453	NN	denotes	production
T6894	5454-5456	IN	denotes	of
T7016	5592-5601	JJ	denotes	Real-time
T7017	5602-5605	NN	denotes	PCR
T7018	5606-5611	JJ	denotes	Total
T7019	5612-5615	NN	denotes	RNA
T7020	5616-5619	VB	denotes	was
T7021	5620-5629	VB	denotes	extracted
T7022	5630-5634	IN	denotes	from
T7023	5635-5640	NN	denotes	cells
T7024	5641-5643	IN	denotes	by
T7025	5644-5649	VB	denotes	using
T7026	5650-5653	DT	denotes	the
T7027	5654-5660	NN	denotes	TRIzol
T7028	5661-5668	NN	denotes	reagent
T7029	5669-5670	-LRB-	denotes	(
T7030	5670-5680	NN	denotes	Invitrogen
T7031	5680-5681	-RRB-	denotes	)
T7032	5681-5682	-COLON-	denotes	;
T7033	5683-5686	NN	denotes	RNA
T7034	5687-5695	NN	denotes	aliquots
T7035	5696-5697	-LRB-	denotes	(
T7036	5697-5703	NN	denotes	200 ng
T7037	5703-5704	-RRB-	denotes	)
T7038	5705-5709	VB	denotes	were
T7039	5710-5717	JJ	denotes	reverse
T7040	5718-5729	VB	denotes	transcribed
T7041	5730-5735	VB	denotes	using
T7042	5736-5742	NNP	denotes	Random
T7043	5743-5750	NNP	denotes	Examers
T7044	5751-5752	-LRB-	denotes	(
T7045	5752-5757	NNP	denotes	Roche
T7046	5757-5758	-RRB-	denotes	)
T7047	5759-5762	CC	denotes	and
T7048	5763-5774	NN	denotes	Superscript
T7049	5775-5778	CD	denotes	III
T7050	5779-5786	JJ	denotes	Reverse
T7051	5787-5800	NN	denotes	Transcriptase
T7052	5801-5802	-LRB-	denotes	(
T7053	5802-5812	NN	denotes	Invitrogen
T7054	5812-5813	-RRB-	denotes	)
T7055	5813-5814	-COMMA-	denotes	,
T7056	5815-5824	VB	denotes	according
T7057	5825-5827	TO	denotes	to
T7058	5828-5831	DT	denotes	the
T7059	5832-5844	NN	denotes	manufacturer
T7060	5844-5846	POS	denotes	's
T7061	5847-5855	NN	denotes	protocol
T7062	5857-5866	JJ	denotes	Real-time
T7063	5867-5870	NN	denotes	PCR
T7064	5871-5874	VB	denotes	was
T7065	5875-5884	VB	denotes	performed
T7066	5885-5889	IN	denotes	with
T7067	5890-5893	DT	denotes	the
T7068	5894-5896	NN	denotes	iQ
T7069	5897-5902	NNP	denotes	Green
T7070	5903-5908	NNP	denotes	Super
T7071	5909-5912	NN	denotes	mix
T7072	5913-5914	-LRB-	denotes	(
T7073	5914-5921	NNP	denotes	Bio-Rad
T7074	5922-5934	NNP	denotes	Laboratories
T7075	5934-5935	-RRB-	denotes	)
T7076	5936-5939	CC	denotes	and
T7077	5940-5947	VB	denotes	carried
T7078	5948-5951	RP	denotes	out
T7079	5952-5956	IN	denotes	with
T7080	5957-5960	DT	denotes	the
T7081	5961-5968	NN	denotes	iCycler
T7082	5969-5971	NN	denotes	iQ
T7083	5972-5981	JJ	denotes	Real-Time
T7084	5982-5991	NN	denotes	detection
T7085	5992-5998	NN	denotes	system
T7086	5999-6000	-LRB-	denotes	(
T7087	6000-6007	NNP	denotes	Bio-Rad
T7088	6008-6020	NNP	denotes	Laboratories
T7089	6020-6021	-RRB-	denotes	)
T7090	6022-6027	IN	denotes	under
T7091	6028-6031	DT	denotes	the
T7092	6032-6041	JJ	denotes	following
T7093	6042-6052	NN	denotes	conditions
T7094	6052-6053	-COLON-	denotes	:
T7095	6054-6058	NN	denotes	95°C
T7096	6058-6059	-COMMA-	denotes	,
T7097	6060-6065	NN	denotes	1 min
T7098	6065-6066	-COLON-	denotes	;
T7099	6067-6068	-LRB-	denotes	(
T7100	6068-6072	NN	denotes	94°C
T7101	6072-6073	-COMMA-	denotes	,
T7102	6074-6078	NN	denotes	10 s
T7103	6078-6079	-COLON-	denotes	;
T7104	6080-6084	NN	denotes	60°C
T7105	6084-6085	-COMMA-	denotes	,
T7106	6086-6090	NN	denotes	30 s
T7107	6090-6091	-RRB-	denotes	)
T7108	6092-6095	CD	denotes	×40
T7109	6097-6104	NN	denotes	Primers
T7110	6105-6108	IN	denotes	for
T7111	6109-6112	NN	denotes	Tat
T7112	6113-6116	CC	denotes	and
T7113	6117-6123	NN	denotes	MIP-1α
T7114	6124-6127	VB	denotes	are
T7115	6128-6134	VB	denotes	listed
T7116	6135-6137	IN	denotes	in
T7117	6138-6151	NNP	denotes	Supplementary
T7118	6152-6157	NNP	denotes	Data.
T7119	6158-6167	NNP	denotes	Real-time
T7120	6168-6171	NN	denotes	PCR
T7121	6172-6174	IN	denotes	of
T7122	6175-6179	NN	denotes	CSF3
T7123	6179-6180	-COMMA-	denotes	,
T7124	6181-6184	NN	denotes	LTA
T7125	6184-6185	-COMMA-	denotes	,
T7126	6186-6192	NN	denotes	NFKBIA
T7127	6192-6193	-COMMA-	denotes	,
T7128	6194-6198	NN	denotes	TLR2
T7129	6198-6199	-COMMA-	denotes	,
T7130	6200-6205	NN	denotes	GAPDH
T7131	6206-6209	CC	denotes	and
T7132	6210-6214	NN	denotes	ACTB
T7133	6215-6218	VB	denotes	was
T7134	6219-6228	VB	denotes	performed
T7135	6229-6234	VB	denotes	using
T7136	6235-6238	DT	denotes	the
T7137	6239-6242	NN	denotes	RT2
T7138	6243-6251	NN	denotes	profiler
T7139	6252-6255	NN	denotes	PCR
T7140	6256-6267	JJ	denotes	Array-Human
T7141	6268-6273	NN	denotes	NF-κB
T7142	6274-6283	NN	denotes	signaling
T7143	6284-6291	NN	denotes	pathway
T7144	6292-6293	-LRB-	denotes	(
T7145	6293-6299	NNP	denotes	QIAGEN
T7146	6300-6308	NNP	denotes	Sciences
T7147	6308-6309	-COMMA-	denotes	,
T7148	6310-6312	NNP	denotes	MD
T7149	6312-6313	-COMMA-	denotes	,
T7150	6314-6317	NNP	denotes	USA
T7151	6317-6318	-RRB-	denotes	)
T7152	6320-6329	NN	denotes	Reactions
T7153	6330-6334	VB	denotes	were
T7154	6335-6342	VB	denotes	carried
T7155	6343-6346	RP	denotes	out
T7156	6347-6349	IN	denotes	in
T7157	6350-6360	NN	denotes	triplicate
T7158	6360-6361	-COMMA-	denotes	,
T7159	6362-6365	CC	denotes	and
T7160	6366-6370	NN	denotes	gene
T7161	6371-6381	NN	denotes	expression
T7162	6382-6388	NN	denotes	levels
T7163	6389-6393	VB	denotes	were
T7164	6394-6404	VB	denotes	calculated
T7165	6405-6413	JJ	denotes	relative
T7166	6414-6416	TO	denotes	to
T7167	6417-6422	NN	denotes	GAPDH
T7168	6423-6427	NN	denotes	mRNA
T7169	6428-6434	NN	denotes	levels
T7170	6435-6437	IN	denotes	as
T7171	6438-6448	JJ	denotes	endogenous
T7172	6449-6456	NN	denotes	control
T7173	6458-6466	JJ	denotes	Relative
T7174	6467-6477	NN	denotes	expression
T7175	6478-6481	VB	denotes	was
T7176	6482-6492	VB	denotes	calculated
T7177	6493-6495	IN	denotes	as
T7178	6496-6497	CD	denotes	2
T7179	6497-6498	-LRB-	denotes	(
T7180	6498-6500	NN	denotes	Ct
T7181	6501-6505	NN	denotes	gene
T7182	6506-6511	IN	denotes	under
T7183	6512-6530	NN	denotes	investigation − Ct
T7184	6531-6536	NN	denotes	GAPDH
T7185	6536-6537	-RRB-	denotes	)
T8159	8923-8934	JJ	denotes	Statistical
T8160	8935-8943	NN	denotes	analysis
T8161	8944-8955	JJ	denotes	Statistical
T8162	8956-8964	NN	denotes	analysis
T8163	8965-8968	VB	denotes	was
T8164	8969-8978	VB	denotes	performed
T8165	8979-8981	IN	denotes	by
T8166	8982-8990	JJ	denotes	two-tail
T8167	8991-8999	JJ	denotes	unpaired
T8168	9000-9007	NN	denotes	Student
T8169	9007-9009	POS	denotes	's
T8170	9010-9016	NN	denotes	t-test
T8171	9018-9022	NN	denotes	Data
T8172	9023-9027	VB	denotes	were
T8173	9028-9036	VB	denotes	reported
T8174	9037-9039	IN	denotes	as
T8175	9040-9050	NN	denotes	means ± SE
T8176	9052-9063	NN	denotes	Differences
T8177	9064-9071	IN	denotes	between
T8178	9072-9075	DT	denotes	the
T8179	9076-9081	NN	denotes	means
T8180	9082-9086	VB	denotes	were
T8181	9087-9097	VB	denotes	considered
T8182	9098-9100	IN	denotes	as
T8183	9101-9114	RB	denotes	statistically
T8184	9115-9126	JJ	denotes	significant
T8185	9127-9129	IN	denotes	at
T8186	9130-9133	DT	denotes	the
T8187	9134-9136	CD	denotes	95
T8188	9136-9137	NN	denotes	%
T8189	9138-9143	NN	denotes	level
T8190	9144-9145	-LRB-	denotes	(
T8191	9145-9147	NN	denotes	P
T8192	9147-9148	SYM	denotes	<
T8193	9148-9153	CD	denotes	0.05
T8194	9153-9154	-RRB-	denotes	)
T6077	3189-3192	CC	denotes	and
T6895	5457-5460	NN	denotes	GST
T6896	5461-5469	NN	denotes	proteins
T6897	5470-5472	IN	denotes	in
T6898	5473-5484	FW	denotes	Escherichia
T6899	5485-5489	FW	denotes	coli
T6900	5490-5496	NN	denotes	strain
T6901	5497-5501	NN	denotes	BL21
T6902	5502-5506	VB	denotes	were
T6903	5507-5516	VB	denotes	performed
T6904	5517-5519	IN	denotes	as
T6905	5520-5530	RB	denotes	previously
T6906	5531-5539	VB	denotes	reported
T6907	5540-5541	-LRB-	denotes	(
T6908	5541-5543	CD	denotes	50
T6909	5543-5544	-RRB-	denotes	)
T6910	5546-5553	NN	denotes	Details
T6911	5554-5557	VB	denotes	are
T6912	5558-5567	VB	denotes	described
T6913	5568-5570	IN	denotes	in
T6914	5571-5584	NNP	denotes	Supplementary
T6915	5585-5589	NNP	denotes	Data
R4014	T4416	T4417	arg1Of	pcDNA-3xHA-IκB-α,","
R4015	T4417	T4419	arg1Of	",",","
R4016	T4418	T4417	arg2Of	p3xFLAG-CMV-Tat,","
R4017	T4419	T4415	arg1Of	",",plasmids
R4018	T4419	T4426	arg1Of	",",","
R4019	T4423	T4422	arg2Of	"22,25,27",(
R4020	T4424	T4422	arg3Of	),(
R4021	T4425	T4419	arg2Of	A,","
R4022	T4425	T4420	arg1Of	A,p3xFLAG-CMV-Tat
R4023	T4425	T4421	arg1Of	A,C
R4024	T4425	T4422	arg1Of	A,(
R4025	T4426	T4433	arg1Of	",",","
R4026	T4430	T4429	arg2Of	"49,52,53,55,56,57",(
R4027	T4431	T4429	arg3Of	),(
R4028	T4432	T4426	arg2Of	A,","
R4029	T4432	T4427	arg1Of	A,p3xFLAG-CMV-Tat
R4030	T4432	T4428	arg1Of	A,R
R4031	T4432	T4429	arg1Of	A,(
R4032	T4433	T4435	arg1Of	",",","
R4033	T4434	T4433	arg2Of	pGEX-2T-Tat,","
R4034	T4435	T4442	arg1Of	",",and
R4035	T4439	T4438	arg2Of	"22,25,27",(
R4036	T4440	T4438	arg3Of	),(
R4037	T4441	T4435	arg2Of	A,","
R4038	T4441	T4436	arg1Of	A,pGEX-2T-Tat
R4039	T4441	T4437	arg1Of	A,C
R4040	T4441	T4438	arg1Of	A,(
R4041	T4442	T4414	arg1Of	and,The
R4042	T4442	T4449	arg1Of	and,were
R4043	T4442	T4451	arg2Of	and,described
R4044	T4446	T4445	arg2Of	"49,52,53,55,56,57",(
R4045	T4447	T4445	arg3Of	),(
R4046	T4448	T4442	arg2Of	A,and
R4047	T4448	T4443	arg1Of	A,pGEX-2T-Tat
R4048	T4448	T4444	arg1Of	A,R
R4049	T4448	T4445	arg1Of	A,(
R4050	T4451	T4449	arg2Of	described,were
R4051	T4451	T4450	arg1Of	described,previously
R4052	T4451	T4452	arg1Of	described,(
R4053	T4453	T4452	arg2Of	50,(
R4054	T4454	T4452	arg3Of	),(
R4055	T4457	T4458	arg1Of	pNL4-3.Luc.R-E-,and
R4056	T4458	T4455	arg1Of	and,The
R4057	T4458	T4456	arg1Of	and,plasmids
R4058	T4458	T4460	arg1Of	and,were
R4059	T4458	T4461	arg2Of	and,obtained
R4060	T4459	T4458	arg2Of	pHXB2-env,and
R4061	T4461	T4460	arg2Of	obtained,were
R4062	T4461	T4462	arg1Of	obtained,from
R4063	T4461	T4480	arg1Of	obtained,;
R4064	T4469	T4462	arg2Of	Program,from
R4065	T4469	T4463	arg1Of	Program,the
R4066	T4469	T4464	arg1Of	Program,AIDS
R4067	T4469	T4465	arg1Of	Program,Research
R4068	T4469	T4466	arg1Of	Program,&
R4069	T4469	T4467	arg1Of	Program,Reference
R4070	T4469	T4468	arg1Of	Program,Reagent
R4071	T4469	T4470	arg1Of	Program,","
R4072	T4469	T4478	arg1Of	Program,","
R4073	T4471	T4470	arg2Of	Division,","
R4074	T4471	T4472	arg1Of	Division,of
R4075	T4473	T4474	arg1Of	AIDS,","
R4076	T4474	T4476	arg1Of	",",","
R4077	T4475	T4474	arg2Of	NIAID,","
R4078	T4476	T4472	arg2Of	",",of
R4079	T4477	T4476	arg2Of	NIH,","
R4080	T4479	T4478	arg2Of	USA,","
R4081	T4481	T4482	arg1Of	pκBluc,and
R4082	T4482	T4484	arg1Of	and,were
R4083	T4482	T4485	arg2Of	and,purchased
R4084	T4483	T4482	arg2Of	pSV-β-Gal,and
R4085	T4485	T4480	arg2Of	purchased,;
R4086	T4485	T4484	arg2Of	purchased,were
R4087	T4485	T4486	arg1Of	purchased,from
R4088	T4487	T4486	arg2Of	Promega,from
R4089	T4487	T4488	arg1Of	Promega,(
R4090	T4489	T4490	arg1Of	Madison,","
R4091	T4490	T4488	arg2Of	",",(
R4092	T4490	T4492	arg1Of	",",","
R4093	T4491	T4490	arg2Of	WI,","
R4094	T4493	T4492	arg2Of	USA,","
R4095	T4494	T4488	arg3Of	),(
R4096	T4497	T4498	arg1Of	pRc/CMV-3xHA-p65,","
R4097	T4498	T4496	arg1Of	",",plasmids
R4098	T4498	T4503	arg1Of	",",","
R4099	T4499	T4498	arg2Of	pRc/CMV-3xHA-p65ΔC,","
R4100	T4499	T4500	arg1Of	pRc/CMV-3xHA-p65ΔC,(
R4101	T4501	T4500	arg2Of	1–318,(
R4102	T4502	T4500	arg3Of	),(
R4103	T4503	T4508	arg1Of	",",","
R4104	T4504	T4503	arg2Of	pRc/CMV-3xHA-p65ΔN,","
R4105	T4504	T4505	arg1Of	pRc/CMV-3xHA-p65ΔN,(
R4106	T4506	T4505	arg2Of	122–551,(
R4107	T4507	T4505	arg3Of	),(
R4108	T4508	T4511	arg1Of	",",","
R4109	T4510	T4508	arg2Of	T,","
R4110	T4510	T4509	arg1Of	T,p3xFLAG-CMV-Tat
R4111	T4511	T4517	arg1Of	",",","
R4112	T4514	T4513	arg2Of	"23,24",(
R4113	T4515	T4513	arg3Of	),(
R4114	T4516	T4511	arg2Of	A,","
R4115	T4516	T4512	arg1Of	A,N
R4116	T4516	T4513	arg1Of	A,(
R4117	T4517	T4495	arg1Of	",",The
R4118	T4517	T4542	arg1Of	",",were
R4119	T4517	T4543	arg2Of	",",generated
R4120	T4521	T4520	arg2Of	"50,51",(
R4121	T4522	T4520	arg3Of	),(
R4122	T4523	T4518	arg1Of	A,p3xFLAG-CMV-Tat
R4123	T4523	T4519	arg1Of	A,K
R4124	T4523	T4520	arg1Of	A,(
R4125	T4523	T4524	arg1Of	A,","
R4126	T4524	T4527	arg1Of	",",","
R4127	T4526	T4524	arg2Of	T,","
R4128	T4526	T4525	arg1Of	T,pGEX-2T-Tat
R4129	T4527	T4533	arg1Of	",",","
R4130	T4530	T4529	arg2Of	"23,24",(
R4131	T4531	T4529	arg3Of	),(
R4132	T4532	T4527	arg2Of	A,","
R4133	T4532	T4528	arg1Of	A,N
R4134	T4532	T4529	arg1Of	A,(
R4135	T4533	T4540	arg1Of	",",and
R4136	T4537	T4536	arg2Of	"50,51",(
R4137	T4538	T4536	arg3Of	),(
R4138	T4539	T4533	arg2Of	A,","
R4139	T4539	T4534	arg1Of	A,pGEX-2T-Tat
R4140	T4539	T4535	arg1Of	A,K
R4141	T4539	T4536	arg1Of	A,(
R4142	T4540	T4517	arg2Of	and,","
R4143	T4541	T4540	arg2Of	pNL4-3.FLAG-Tat.R-E-,and
R4144	T4543	T4542	arg2Of	generated,were
R4145	T4543	T4544	arg1Of	generated,as
R4146	T4545	T4544	arg2Of	described,as
R4147	T4545	T4546	arg1Of	described,in
R4148	T4548	T4546	arg2Of	Data,in
R4149	T4548	T4547	arg1Of	Data,Supplementary
R4308	T4915	T4916	arg1Of	Cells,","
R4309	T4916	T4918	arg1Of	",",","
R4310	T4917	T4916	arg2Of	transfection,","
R4311	T4918	T4920	arg1Of	",",and
R4312	T4919	T4918	arg2Of	treatments,","
R4313	T4922	T4920	arg2Of	assay,and
R4314	T4922	T4921	arg1Of	assay,luciferase
R4315	T4923	T4924	arg1Of	HeLa,","
R4316	T4925	T4924	arg2Of	p50−/−p65−/−,","
R4317	T4928	T4923	arg1Of	fibroblasts,HeLa
R4318	T4928	T4925	arg1Of	fibroblasts,p50−/−p65−/−
R4319	T4928	T4926	arg1Of	fibroblasts,mouse
R4320	T4928	T4927	arg1Of	fibroblasts,embryonic
R4321	T4928	T4929	arg1Of	fibroblasts,(
R4322	T4928	T4932	arg1Of	fibroblasts,(
R4323	T4928	T4935	arg1Of	fibroblasts,and
R4324	T4930	T4929	arg2Of	MEFs,(
R4325	T4931	T4929	arg3Of	),(
R4326	T4933	T4932	arg2Of	52,(
R4327	T4934	T4932	arg3Of	),(
R4328	T4935	T4938	arg1Of	and,were
R4329	T4935	T4939	arg2Of	and,cultured
R4330	T4937	T4935	arg2Of	cells,and
R4331	T4937	T4936	arg1Of	cells,293T
R4332	T4939	T4938	arg2Of	cultured,were
R4333	T4939	T4940	arg1Of	cultured,in
R4334	T4939	T4947	arg1Of	cultured,;
R4335	T4941	T4942	arg2Of	Dulbecco,'s
R4336	T4944	T4942	arg1Of	Eagle,'s
R4337	T4944	T4943	arg2Of	Eagle,modified
R4338	T4944	T4945	arg2Of	Eagle,'s
R4339	T4946	T4940	arg2Of	medium,in
R4340	T4946	T4945	arg1Of	medium,'s
R4341	T4948	T4949	arg1Of	Jurkat,","
R4342	T4949	T4952	arg1Of	",",and
R4343	T4951	T4949	arg2Of	cells,","
R4344	T4951	T4950	arg1Of	cells,U937
R4345	T4952	T4958	arg1Of	and,(
R4346	T4952	T4961	arg1Of	and,were
R4347	T4952	T4962	arg2Of	and,cultured
R4348	T4957	T4952	arg2Of	cells,and
R4349	T4957	T4953	arg1Of	cells,human
R4350	T4957	T4954	arg1Of	cells,peripheral
R4351	T4957	T4955	arg1Of	cells,blood
R4352	T4957	T4956	arg1Of	cells,mononuclear
R4353	T4959	T4958	arg2Of	PBMCs,(
R4354	T4960	T4958	arg3Of	),(
R4355	T4962	T4938	modOf	cultured,were
R4356	T4962	T4961	arg2Of	cultured,were
R4357	T4962	T4963	arg1Of	cultured,in
R4358	T4964	T4963	arg2Of	RPMI,in
R4359	T4964	T4965	arg1Of	RPMI,1640
R4360	T4966	T4967	arg1Of	PBMCs,were
R4361	T4966	T4968	arg2Of	PBMCs,isolated
R4362	T4968	T4967	arg2Of	isolated,were
R4363	T4968	T4969	arg1Of	isolated,as
R4364	T4971	T4969	arg2Of	described,as
R4365	T4971	T4970	arg1Of	described,previously
R4366	T4971	T4972	arg1Of	described,(
R4367	T4973	T4972	arg2Of	53,(
R4368	T4974	T4972	arg3Of	),(
R4369	T4975	T4976	arg1Of	Media,were
R4370	T4975	T4977	arg2Of	Media,supplemented
R4371	T4977	T4976	arg2Of	supplemented,were
R4372	T4977	T4978	arg1Of	supplemented,with
R4373	T4979	T4980	arg1Of	10,%
R4374	T4984	T4981	arg1Of	serum,heat-inactivated
R4375	T4984	T4982	arg1Of	serum,fetal
R4376	T4984	T4983	arg1Of	serum,calf
R4377	T4984	T4985	arg1Of	serum,and
R4378	T4985	T4978	arg2Of	and,with
R4379	T4985	T4979	arg1Of	and,10
R4380	T4985	T4988	arg1Of	and,(
R4381	T4987	T4985	arg2Of	l-glutamine,and
R4382	T4987	T4986	arg1Of	l-glutamine,2 mM
R4383	T4991	T4988	arg2Of	AG,(
R4384	T4991	T4989	arg1Of	AG,Lonza
R4385	T4991	T4990	arg1Of	AG,Cologne
R4386	T4991	T4992	arg1Of	AG,","
R4387	T4993	T4992	arg2Of	Germany,","
R4388	T4994	T4988	arg3Of	),(
R4389	T4995	T4996	arg1Of	HeLa,","
R4390	T4996	T4999	arg1Of	",",and
R4391	T4998	T4996	arg2Of	MEFs,","
R4392	T4998	T4997	arg1Of	MEFs,p50−/−p65−/−
R4393	T4999	T5001	arg1Of	and,were
R4394	T4999	T5002	arg2Of	and,transfected
R4395	T5000	T4999	arg2Of	293T,and
R4396	T5002	T5001	arg2Of	transfected,were
R4397	T5002	T5003	arg1Of	transfected,with
R4398	T5002	T5005	arg1Of	transfected,by
R4399	T5002	T5018	arg1Of	transfected,","
R4400	T5002	T5019	arg1Of	transfected,according
R4401	T5002	T5025	arg1Of	transfected,;
R4402	T5004	T5003	arg2Of	DNA,with
R4403	T5006	T5005	arg2Of	using,by
R4404	T5008	T5006	arg2Of	HD,using
R4405	T5008	T5007	arg1Of	HD,FuGENE
R4406	T5008	T5009	arg1Of	HD,(
R4407	T5012	T5009	arg2Of	GmbH,(
R4408	T5012	T5010	arg1Of	GmbH,Roche
R4409	T5012	T5011	arg1Of	GmbH,Diagnostic
R4410	T5012	T5013	arg1Of	GmbH,","
R4411	T5014	T5013	arg2Of	Mannheim,","
R4412	T5014	T5015	arg1Of	Mannheim,","
R4413	T5016	T5015	arg2Of	Germany,","
R4414	T5017	T5009	arg3Of	),(
R4415	T5020	T5019	arg2Of	to,according
R4416	T5023	T5020	arg2Of	's,to
R4417	T5023	T5021	arg1Of	's,the
R4418	T5023	T5022	arg1Of	's,manufacturer
R4419	T5023	T5024	arg1Of	's,protocol
R4420	T5028	T5026	arg1Of	amounts,total
R4421	T5028	T5027	arg1Of	amounts,DNA
R4422	T5028	T5029	arg1Of	amounts,were
R4423	T5028	T5030	arg2Of	amounts,equalized
R4424	T5030	T5025	arg2Of	equalized,;
R4425	T5030	T5029	arg2Of	equalized,were
R4426	T5032	T5030	arg1Of	transfection,equalized
R4427	T5032	T5031	arg2Of	transfection,by
R4428	T5032	T5033	arg1Of	transfection,of
R4429	T5036	T5033	arg2Of	vector,of
R4430	T5036	T5034	arg1Of	vector,pRc/CMV
R4431	T5036	T5035	arg1Of	vector,empty
R4432	T5036	T5037	arg1Of	vector,(
R4433	T5038	T5037	arg2Of	Invitrogen,(
R4434	T5038	T5039	arg1Of	Invitrogen,","
R4435	T5038	T5043	arg1Of	Invitrogen,","
R4436	T5038	T5044	arg1Of	Invitrogen,USA
R4437	T5040	T5041	arg1Of	Carlsbad,","
R4438	T5041	T5039	arg2Of	",",","
R4439	T5042	T5041	arg2Of	CA,","
R4440	T5045	T5037	arg3Of	),(
R4441	T5047	T5046	arg2Of	pulse-stimulation,For
R4442	T5050	T5049	arg1Of	cells,HeLa
R4443	T5050	T5051	arg1Of	cells,were
R4444	T5050	T5052	arg2Of	cells,treated
R4445	T5050	T5098	arg1Of	cells,returned
R4446	T5052	T5051	arg2Of	treated,were
R4447	T5052	T5053	arg1Of	treated,with
R4448	T5052	T5096	arg1Of	treated,and
R4449	T5056	T5054	arg1Of	13-acetate,phorbol
R4450	T5056	T5055	arg1Of	13-acetate,12-myristate
R4451	T5056	T5057	arg1Of	13-acetate,(
R4452	T5056	T5069	arg1Of	13-acetate,(
R4453	T5056	T5072	arg1Of	13-acetate,for
R4454	T5056	T5075	arg1Of	13-acetate,or
R4455	T5058	T5057	arg2Of	PMA,(
R4456	T5058	T5059	arg1Of	PMA,;
R4457	T5060	T5059	arg2Of	Sigma-Aldrich,;
R4458	T5060	T5061	arg1Of	Sigma-Aldrich,","
R4459	T5060	T5066	arg1Of	Sigma-Aldrich,","
R4460	T5060	T5067	arg1Of	Sigma-Aldrich,USA
R4461	T5063	T5061	arg2Of	Louis,","
R4462	T5063	T5062	arg1Of	Louis,St
R4463	T5063	T5064	arg1Of	Louis,","
R4464	T5065	T5064	arg2Of	MO,","
R4465	T5068	T5057	arg3Of	),(
R4466	T5070	T5069	arg2Of	20 ng/ml,(
R4467	T5071	T5069	arg3Of	),(
R4468	T5073	T5072	arg2Of	5 min,for
R4469	T5075	T5053	arg2Of	or,with
R4470	T5075	T5074	arg1Of	or,","
R4471	T5078	T5075	arg2Of	factor-α,or
R4472	T5078	T5076	arg1Of	factor-α,tumor
R4473	T5078	T5077	arg1Of	factor-α,necrosis
R4474	T5078	T5079	arg1Of	factor-α,(
R4475	T5078	T5084	arg1Of	factor-α,(
R4476	T5078	T5087	arg1Of	factor-α,for
R4477	T5078	T5089	arg1Of	factor-α,","
R4478	T5078	T5090	arg2Of	factor-α,washed
R4479	T5080	T5079	arg2Of	TNF-α,(
R4480	T5080	T5081	arg1Of	TNF-α,;
R4481	T5080	T5082	arg1Of	TNF-α,Sigma-Aldrich
R4482	T5083	T5079	arg3Of	),(
R4483	T5085	T5084	arg2Of	20 ng/ml,(
R4484	T5086	T5084	arg3Of	),(
R4485	T5088	T5087	arg2Of	30 min,for
R4486	T5090	T5091	arg1Of	washed,twice
R4487	T5090	T5092	arg1Of	washed,in
R4488	T5095	T5092	arg2Of	medium,in
R4489	T5095	T5093	arg1Of	medium,complete
R4490	T5095	T5094	arg1Of	medium,culture
R4491	T5096	T5046	arg1Of	and,For
R4492	T5096	T5048	arg1Of	and,","
R4493	T5098	T5096	arg2Of	returned,and
R4494	T5098	T5097	arg1Of	returned,then
R4495	T5098	T5099	arg1Of	returned,to
R4496	T5100	T5099	arg2Of	culture,to
R4497	T5103	T5101	arg2Of	assays,For
R4498	T5103	T5102	arg1Of	assays,luciferase
R4499	T5105	T5106	arg1Of	pSV-β-Gal,was
R4500	T5105	T5107	arg2Of	pSV-β-Gal,co-transfected
R4501	T5107	T5101	arg1Of	co-transfected,For
R4502	T5107	T5104	arg1Of	co-transfected,","
R4503	T5107	T5106	arg2Of	co-transfected,was
R4504	T5107	T5108	arg1Of	co-transfected,with
R4505	T5107	T5110	modOf	co-transfected,to
R4506	T5109	T5108	arg2Of	pκBluc,with
R4507	T5111	T5110	arg1Of	monitor,to
R4508	T5114	T5111	arg2Of	efficiency,monitor
R4509	T5114	T5112	arg1Of	efficiency,the
R4510	T5114	T5113	arg1Of	efficiency,transfection
R4511	T5118	T5115	arg1Of	cells,Forty-eight-hour
R4512	T5118	T5116	arg1Of	cells,post-transfection
R4513	T5118	T5117	arg1Of	cells,","
R4514	T5118	T5119	arg1Of	cells,were
R4515	T5118	T5120	arg2Of	cells,lysed
R4516	T5120	T5119	arg2Of	lysed,were
R4517	T5120	T5121	arg1Of	lysed,in
R4518	T5120	T5138	arg1Of	lysed,and
R4519	T5123	T5121	arg2Of	buffer,in
R4520	T5123	T5122	arg1Of	buffer,lysis
R4521	T5123	T5124	arg1Of	buffer,of
R4522	T5128	T5124	arg2Of	System,of
R4523	T5128	T5125	arg1Of	System,Dual
R4524	T5128	T5126	arg1Of	System,Light
R4525	T5128	T5127	arg1Of	System,Luciferase
R4526	T5128	T5129	arg1Of	System,(
R4527	T5130	T5131	arg1Of	Tropix,","
R4528	T5131	T5133	arg1Of	",",","
R4529	T5132	T5131	arg2Of	Bedford,","
R4530	T5133	T5129	arg2Of	",",(
R4531	T5133	T5135	arg1Of	",",","
R4532	T5134	T5133	arg2Of	MA,","
R4533	T5136	T5135	arg2Of	USA,","
R4534	T5137	T5129	arg3Of	),(
R4535	T5140	T5141	arg1Of	luciferase,and
R4536	T5142	T5141	arg2Of	β-galactosidase,and
R4537	T5143	T5139	arg1Of	activities,the
R4538	T5143	T5140	arg1Of	activities,luciferase
R4539	T5143	T5142	arg1Of	activities,β-galactosidase
R4540	T5143	T5144	arg1Of	activities,were
R4541	T5143	T5145	arg2Of	activities,evaluated
R4542	T5145	T5138	arg2Of	evaluated,and
R4543	T5145	T5144	arg2Of	evaluated,were
R4544	T5145	T5146	arg1Of	evaluated,by
R4545	T5147	T5146	arg2Of	using,by
R4546	T5147	T5155	arg1Of	using,in
R4547	T5147	T5158	arg1Of	using,(
R4548	T5151	T5147	arg2Of	System,using
R4549	T5151	T5148	arg1Of	System,Dual
R4550	T5151	T5149	arg1Of	System,Light
R4551	T5151	T5150	arg1Of	System,Luciferase
R4552	T5151	T5152	arg1Of	System,(
R4553	T5153	T5152	arg2Of	Tropix,(
R4554	T5154	T5152	arg3Of	),(
R4555	T5157	T5155	arg2Of	bioluminometer,in
R4556	T5157	T5156	arg1Of	bioluminometer,a
R4557	T5160	T5158	arg2Of	Biosystem,(
R4558	T5160	T5159	arg1Of	Biosystem,Turner
R4559	T5160	T5161	arg1Of	Biosystem,","
R4560	T5160	T5165	arg1Of	Biosystem,","
R4561	T5160	T5166	arg1Of	Biosystem,USA
R4562	T5162	T5161	arg2Of	Sunnyvale,","
R4563	T5162	T5163	arg1Of	Sunnyvale,","
R4564	T5164	T5163	arg2Of	CA,","
R4565	T5167	T5158	arg3Of	),(
R4566	T5169	T5168	arg1Of	ratio,The
R4567	T5169	T5170	arg1Of	ratio,of
R4568	T5169	T5174	arg1Of	ratio,to
R4569	T5169	T5177	arg1Of	ratio,was
R4570	T5169	T5178	arg2Of	ratio,expressed
R4571	T5173	T5170	arg2Of	activity,of
R4572	T5173	T5171	arg1Of	activity,firefly
R4573	T5173	T5172	arg1Of	activity,luciferase
R4574	T5176	T5174	arg2Of	activity,to
R4575	T5176	T5175	arg1Of	activity,β-galactosidase
R4576	T5178	T5177	arg2Of	expressed,was
R4577	T5178	T5179	arg1Of	expressed,as
R4578	T5182	T5179	arg2Of	units,as
R4579	T5182	T5180	arg1Of	units,relative
R4580	T5182	T5181	arg1Of	units,light
R4893	T5683	T5682	arg1Of	interference,RNA
R4894	T5684	T5685	arg1Of	Jurkat,or
R4895	T5686	T5685	arg2Of	U937,or
R4896	T5687	T5684	arg1Of	cells,Jurkat
R4897	T5687	T5686	arg1Of	cells,U937
R4898	T5687	T5688	arg1Of	cells,were
R4899	T5687	T5689	arg2Of	cells,transfected
R4900	T5689	T5688	arg2Of	transfected,were
R4901	T5691	T5689	arg1Of	electroporation,transfected
R4902	T5691	T5690	arg2Of	electroporation,by
R4903	T5691	T5692	arg1Of	electroporation,using
R4904	T5695	T5692	arg2Of	apparatus,using
R4905	T5695	T5693	arg1Of	apparatus,a
R4906	T5695	T5694	arg1Of	apparatus,Bio-Rad
R4907	T5695	T5696	arg1Of	apparatus,(
R4908	T5698	T5697	arg1Of	Laboratories,Bio-Rad
R4909	T5698	T5699	arg1Of	Laboratories,","
R4910	T5699	T5701	arg1Of	",",","
R4911	T5700	T5699	arg2Of	Hercules,","
R4912	T5701	T5696	arg2Of	",",(
R4913	T5701	T5703	arg1Of	",",","
R4914	T5701	T5704	arg1Of	",",USA
R4915	T5702	T5701	arg2Of	CA,","
R4916	T5705	T5696	arg3Of	),(
R4917	T5708	T5709	arg1Of	aliquots,(
R4918	T5708	T5713	arg1Of	aliquots,were
R4919	T5708	T5714	arg2Of	aliquots,suspended
R4920	T5708	T5728	arg2Of	aliquots,subjected
R4921	T5711	T5709	arg2Of	cells,(
R4922	T5711	T5710	arg1Of	cells,5 × 106
R4923	T5712	T5709	arg3Of	),(
R4924	T5714	T5715	arg1Of	suspended,in
R4925	T5714	T5727	arg1Of	suspended,and
R4926	T5716	T5715	arg2Of	0.3 ml,in
R4927	T5716	T5717	arg1Of	0.3 ml,of
R4928	T5716	T5720	arg2Of	0.3 ml,supplemented
R4929	T5718	T5717	arg2Of	RPMI,of
R4930	T5718	T5719	arg1Of	RPMI,1640
R4931	T5720	T5721	arg1Of	supplemented,with
R4932	T5722	T5723	arg1Of	20,%
R4933	T5726	T5721	arg2Of	serum,with
R4934	T5726	T5722	arg1Of	serum,20
R4935	T5726	T5724	arg1Of	serum,fetal
R4936	T5726	T5725	arg1Of	serum,calf
R4937	T5727	T5706	arg1Of	and,Briefly
R4938	T5727	T5707	arg1Of	and,","
R4939	T5727	T5713	arg2Of	and,were
R4940	T5727	T5748	arg1Of	and,;
R4941	T5728	T5727	arg2Of	subjected,and
R4942	T5728	T5729	arg1Of	subjected,to
R4943	T5728	T5739	arg1Of	subjected,in
R4944	T5733	T5729	arg2Of	pulse,to
R4945	T5733	T5730	arg1Of	pulse,a
R4946	T5733	T5731	arg1Of	pulse,double
R4947	T5733	T5732	arg1Of	pulse,electrical
R4948	T5733	T5734	arg1Of	pulse,(
R4949	T5735	T5734	arg2Of	0.22 V,(
R4950	T5735	T5736	arg1Of	0.22 V,","
R4951	T5737	T5736	arg2Of	960 µF,","
R4952	T5738	T5734	arg3Of	),(
R4953	T5739	T5740	arg1Of	in,the
R4954	T5739	T5742	arg1Of	in,of
R4955	T5741	T5739	arg2Of	presence,in
R4956	T5744	T5739	arg3Of	siRNA,in
R4957	T5744	T5743	arg2Of	siRNA,annealed
R4958	T5744	T5745	arg1Of	siRNA,(
R4959	T5746	T5745	arg2Of	200 pmol,(
R4960	T5747	T5745	arg3Of	),(
R4961	T5750	T5749	arg2Of	cells,electroporated
R4962	T5750	T5751	arg1Of	cells,were
R4963	T5750	T5752	arg2Of	cells,washed
R4964	T5750	T5754	arg2Of	cells,cultured
R4965	T5752	T5753	arg1Of	washed,and
R4966	T5753	T5748	arg2Of	and,;
R4967	T5753	T5751	arg2Of	and,were
R4968	T5753	T5755	arg1Of	and,in
R4969	T5754	T5753	arg2Of	cultured,and
R4970	T5757	T5755	arg2Of	medium,in
R4971	T5757	T5756	arg1Of	medium,complete
R4972	T5759	T5758	arg1Of	interference,RNA
R4973	T5759	T5760	arg1Of	interference,was
R4974	T5759	T5761	arg2Of	interference,performed
R4975	T5761	T5760	arg2Of	performed,was
R4976	T5761	T5762	arg1Of	performed,with
R4977	T5761	T5787	arg1Of	performed,UAGCAAUUGUGACAAGCAGUU
R4978	T5762	T5763	arg1Of	with,:
R4979	T5766	T5764	arg1Of	sense,siRNA
R4980	T5766	T5765	arg1Of	sense,Tat
R4981	T5766	T5767	arg1Of	sense,","
R4982	T5767	T5769	arg1Of	",",and
R4983	T5768	T5767	arg2Of	CUGCUUGUACCAAUUGCUAUU,","
R4984	T5771	T5769	arg2Of	Tat,and
R4985	T5771	T5770	arg1Of	Tat,siRNA
R4986	T5772	T5773	arg1Of	antisense,","
R4987	T5774	T5772	arg1Of	UAGCAAUUGGUACAAGCAGUU,antisense
R4988	T5774	T5775	arg1Of	UAGCAAUUGGUACAAGCAGUU,;
R4989	T5775	T5779	arg1Of	;,","
R4990	T5778	T5775	arg2Of	sense,;
R4991	T5778	T5776	arg1Of	sense,siRNA
R4992	T5778	T5777	arg1Of	sense,control
R4993	T5779	T5766	arg1Of	",",sense
R4994	T5779	T5768	arg1Of	",",CUGCUUGUACCAAUUGCUAUU
R4995	T5779	T5771	arg1Of	",",Tat
R4996	T5779	T5782	arg1Of	",",and
R4997	T5781	T5779	arg2Of	AUUGCUAUU,","
R4998	T5781	T5780	arg1Of	AUUGCUAUU,CUGCUUGUCACA
R4999	T5782	T5762	arg2Of	and,with
R5000	T5784	T5782	arg2Of	control,and
R5001	T5784	T5783	arg1Of	control,siRNA
R5002	T5785	T5786	arg1Of	antisense,","
R5003	T5787	T5785	arg1Of	UAGCAAUUGUGACAAGCAGUU,antisense
R5004	T5789	T5788	arg1Of	interference,RNA
R5005	T5789	T5790	arg1Of	interference,of
R5006	T5789	T5794	arg1Of	interference,was
R5007	T5789	T5795	arg2Of	interference,performed
R5008	T5791	T5792	arg1Of	p65,and
R5009	T5792	T5790	arg2Of	and,of
R5010	T5793	T5792	arg2Of	IκB-α,and
R5011	T5795	T5794	arg2Of	performed,was
R5012	T5795	T5796	arg1Of	performed,with
R5013	T5800	T5797	arg1Of	p65,SMART
R5014	T5800	T5798	arg1Of	p65,pool
R5015	T5800	T5799	arg1Of	p65,siRNA
R5016	T5800	T5801	arg1Of	p65,and
R5017	T5801	T5796	arg2Of	and,with
R5018	T5802	T5801	arg2Of	IκB-α,and
R5019	T5802	T5803	arg1Of	IκB-α,(
R5020	T5804	T5805	arg1Of	Dharmacon,","
R5021	T5805	T5807	arg1Of	",",","
R5022	T5806	T5805	arg2Of	Chicago,","
R5023	T5807	T5803	arg2Of	",",(
R5024	T5807	T5809	arg1Of	",",","
R5025	T5807	T5810	arg1Of	",",USA
R5026	T5808	T5807	arg2Of	IL,","
R5027	T5811	T5803	arg3Of	),(
R5180	T6050	T6049	arg2Of	virions,Pseudotyped
R5181	T6050	T6051	arg1Of	virions,and
R5182	T6054	T6051	arg2Of	infection,and
R5183	T6054	T6052	arg1Of	infection,single
R5184	T6054	T6053	arg1Of	infection,round
R5185	T6056	T6055	arg1Of	cells,293T
R5186	T6056	T6057	arg1Of	cells,(
R5187	T6056	T6060	arg1Of	cells,were
R5188	T6056	T6061	arg2Of	cells,transfected
R5189	T6058	T6057	arg2Of	1 × 107,(
R5190	T6059	T6057	arg3Of	),(
R5191	T6061	T6060	arg2Of	transfected,were
R5192	T6061	T6062	arg1Of	transfected,with
R5193	T6061	T6077	arg1Of	transfected,and
R5194	T6063	T6064	arg1Of	pNL4-3.Luc.R-E-,or
R5195	T6064	T6062	arg2Of	or,with
R5196	T6065	T6064	arg2Of	pNL4-3.FLAG-Tat.R-E-,or
R5197	T6065	T6066	arg1Of	pNL4-3.FLAG-Tat.R-E-,(
R5198	T6065	T6070	arg1Of	pNL4-3.FLAG-Tat.R-E-,with
R5199	T6067	T6066	arg2Of	10 µg,(
R5200	T6068	T6066	arg3Of	),(
R5201	T6070	T6069	arg1Of	with,together
R5202	T6072	T6070	arg2Of	Env,with
R5203	T6072	T6071	arg1Of	Env,pHXB2
R5204	T6072	T6073	arg1Of	Env,(
R5205	T6074	T6073	arg2Of	10 µg,(
R5206	T6075	T6073	arg3Of	),(
R5207	T6077	T6076	arg1Of	and,","
R5208	T6081	T6078	arg1Of	supernatant,48-h
R5209	T6081	T6079	arg1Of	supernatant,post-transfection
R5210	T6081	T6080	arg1Of	supernatant,cell
R5211	T6081	T6082	arg1Of	supernatant,was
R5212	T6081	T6083	arg2Of	supernatant,collected
R5213	T6083	T6077	arg2Of	collected,and
R5214	T6083	T6082	arg2Of	collected,was
R5215	T6086	T6084	arg1Of	assay,Enzyme-linked
R5216	T6086	T6085	arg1Of	assay,immunosorbent
R5217	T6086	T6087	arg1Of	assay,(
R5218	T6086	T6090	arg1Of	assay,using
R5219	T6088	T6087	arg2Of	ELISA,(
R5220	T6089	T6087	arg3Of	),(
R5221	T6095	T6090	arg2Of	concentration,using
R5222	T6095	T6091	arg1Of	concentration,anti-p24
R5223	T6095	T6092	arg1Of	concentration,antibody
R5224	T6095	T6093	arg2Of	concentration,measured
R5225	T6095	T6094	arg1Of	concentration,virion
R5226	T6096	T6097	arg1Of	PBMCs,","
R5227	T6097	T6099	arg1Of	",",or
R5228	T6098	T6097	arg2Of	Jurkat,","
R5229	T6099	T6102	arg1Of	or,(
R5230	T6099	T6105	arg1Of	or,were
R5231	T6099	T6106	arg2Of	or,infected
R5232	T6101	T6099	arg2Of	cells,or
R5233	T6101	T6100	arg1Of	cells,U937
R5234	T6103	T6102	arg2Of	5 × 107,(
R5235	T6104	T6102	arg3Of	),(
R5236	T6106	T6105	arg2Of	infected,were
R5237	T6106	T6107	arg1Of	infected,with
R5238	T6110	T6107	arg2Of	virions,with
R5239	T6110	T6108	arg1Of	virions,HXB2
R5240	T6110	T6109	arg1Of	virions,Env-pseudotyped
R5241	T6110	T6111	arg1Of	virions,(
R5242	T6110	T6116	arg1Of	virions,by
R5243	T6110	T6118	arg1Of	virions,","
R5244	T6110	T6119	arg1Of	virions,as
R5245	T6112	T6111	arg2Of	500 ng,(
R5246	T6112	T6113	arg1Of	500 ng,of
R5247	T6114	T6113	arg2Of	p24,of
R5248	T6115	T6111	arg3Of	),(
R5249	T6117	T6116	arg2Of	spinoculation,by
R5250	T6121	T6119	arg2Of	described,as
R5251	T6121	T6120	arg1Of	described,previously
R5252	T6121	T6122	arg1Of	described,(
R5253	T6123	T6122	arg2Of	50,(
R5254	T6124	T6122	arg3Of	),(
R5707	T6719	T6718	arg1Of	vitro,In
R5708	T6720	T6719	arg1Of	translation,vitro
R5709	T6721	T6722	arg1Of	HA-IκB-α,","
R5710	T6722	T6724	arg1Of	",",and
R5711	T6723	T6722	arg2Of	p65,","
R5712	T6724	T6726	arg1Of	and,were
R5713	T6724	T6727	arg2Of	and,expressed
R5714	T6724	T6735	arg2Of	and,translated
R5715	T6725	T6724	arg2Of	Tat,and
R5716	T6727	T6728	arg1Of	expressed,under
R5717	T6727	T6732	arg1Of	expressed,and
R5718	T6731	T6728	arg2Of	promoter,under
R5719	T6731	T6729	arg1Of	promoter,the
R5720	T6731	T6730	arg1Of	promoter,T7
R5721	T6732	T6726	arg2Of	and,were
R5722	T6734	T6733	arg1Of	vitro,in
R5723	T6735	T6732	arg2Of	translated,and
R5724	T6735	T6734	arg1Of	translated,vitro
R5725	T6735	T6736	modOf	translated,using
R5726	T6742	T6736	arg2Of	system,using
R5727	T6742	T6737	arg1Of	system,the
R5728	T6742	T6738	arg1Of	system,TnT
R5729	T6742	T6739	arg1Of	system,quick
R5730	T6742	T6740	arg2Of	system,coupled
R5731	T6742	T6741	arg1Of	system,transcription/translation
R5732	T6742	T6743	arg1Of	system,(
R5733	T6742	T6746	arg1Of	system,","
R5734	T6742	T6747	arg1Of	system,as
R5735	T6744	T6743	arg2Of	Promega,(
R5736	T6745	T6743	arg3Of	),(
R5737	T6749	T6747	arg2Of	reported,as
R5738	T6749	T6748	arg1Of	reported,previously
R5739	T6749	T6750	arg1Of	reported,(
R5740	T6751	T6750	arg2Of	50,(
R5741	T6752	T6750	arg3Of	),(
R5742	T6753	T6754	arg1Of	Details,are
R5743	T6753	T6755	arg2Of	Details,described
R5744	T6755	T6754	arg2Of	described,are
R5745	T6755	T6756	arg1Of	described,in
R5746	T6758	T6756	arg2Of	Data,in
R5747	T6758	T6757	arg1Of	Data,Supplementary
R5827	T6883	T6882	arg1Of	assay,Immunoprecipitation
R5828	T6883	T6884	arg1Of	assay,and
R5829	T6885	T6884	arg2Of	GST-pull,and
R5830	T6885	T6886	arg1Of	GST-pull,down
R5831	T6887	T6888	arg1Of	Immunoprecipitation,","
R5832	T6888	T6890	arg1Of	",",down
R5833	T6888	T6892	arg1Of	",",and
R5834	T6889	T6888	arg2Of	GST-pull,","
R5835	T6892	T6891	arg1Of	and,","
R5836	T6892	T6894	arg1Of	and,of
R5837	T6892	T6897	arg1Of	and,in
R5838	T6892	T6902	arg1Of	and,were
R5839	T6892	T6903	arg2Of	and,performed
R5840	T6893	T6892	arg2Of	production,and
R5841	T6896	T6894	arg2Of	proteins,of
R5842	T6896	T6895	arg1Of	proteins,GST
R5843	T6901	T6897	arg2Of	BL21,in
R5844	T6901	T6898	arg1Of	BL21,Escherichia
R5845	T6901	T6899	arg1Of	BL21,coli
R5846	T6901	T6900	arg1Of	BL21,strain
R5847	T6903	T6902	arg2Of	performed,were
R5848	T6903	T6904	arg1Of	performed,as
R5849	T6906	T6904	arg2Of	reported,as
R5850	T6906	T6905	arg1Of	reported,previously
R5851	T6906	T6907	arg1Of	reported,(
R5899	T7017	T7016	arg1Of	PCR,Real-time
R5900	T7019	T7018	arg1Of	RNA,Total
R5901	T7019	T7020	arg1Of	RNA,was
R5902	T7019	T7021	arg2Of	RNA,extracted
R5903	T7019	T7025	arg1Of	RNA,using
R5904	T7021	T7020	arg2Of	extracted,was
R5905	T7021	T7022	arg1Of	extracted,from
R5906	T7021	T7024	arg1Of	extracted,by
R5907	T7021	T7032	arg1Of	extracted,;
R5908	T7023	T7022	arg2Of	cells,from
R5909	T7025	T7024	arg2Of	using,by
R5910	T7028	T7025	arg2Of	reagent,using
R5911	T7028	T7026	arg1Of	reagent,the
R5912	T7028	T7027	arg1Of	reagent,TRIzol
R5913	T7028	T7029	arg1Of	reagent,(
R5914	T7030	T7029	arg2Of	Invitrogen,(
R5915	T7031	T7029	arg3Of	),(
R5916	T7034	T7033	arg1Of	aliquots,RNA
R5917	T7034	T7035	arg1Of	aliquots,(
R5918	T7034	T7038	arg1Of	aliquots,were
R5919	T7036	T7035	arg2Of	200 ng,(
R5920	T7037	T7035	arg3Of	),(
R5921	T7038	T7032	arg2Of	were,;
R5922	T7038	T7055	arg1Of	were,","
R5923	T7038	T7056	arg1Of	were,according
R5924	T7043	T7039	arg1Of	Examers,reverse
R5925	T7043	T7040	arg2Of	Examers,transcribed
R5926	T7043	T7041	arg1Of	Examers,using
R5927	T7043	T7042	arg1Of	Examers,Random
R5928	T7043	T7044	arg1Of	Examers,(
R5929	T7043	T7047	arg1Of	Examers,and
R5930	T7045	T7044	arg2Of	Roche,(
R5931	T7046	T7044	arg3Of	),(
R5932	T7047	T7038	arg2Of	and,were
R5933	T7051	T7047	arg2Of	Transcriptase,and
R5934	T7051	T7048	arg1Of	Transcriptase,Superscript
R5935	T7051	T7049	arg1Of	Transcriptase,III
R5936	T7051	T7050	arg1Of	Transcriptase,Reverse
R5937	T7051	T7052	arg1Of	Transcriptase,(
R5938	T7053	T7052	arg2Of	Invitrogen,(
R5939	T7054	T7052	arg3Of	),(
R5940	T7057	T7056	arg2Of	to,according
R5941	T7059	T7058	arg1Of	manufacturer,the
R5942	T7059	T7060	arg2Of	manufacturer,'s
R5943	T7061	T7057	arg2Of	protocol,to
R5944	T7061	T7060	arg1Of	protocol,'s
R5945	T7063	T7062	arg1Of	PCR,Real-time
R5946	T7063	T7064	arg1Of	PCR,was
R5947	T7063	T7065	arg2Of	PCR,performed
R5948	T7063	T7077	arg1Of	PCR,carried
R5949	T7065	T7064	arg2Of	performed,was
R5950	T7065	T7066	arg1Of	performed,with
R5951	T7065	T7076	arg1Of	performed,and
R5952	T7070	T7069	arg1Of	Super,Green
R5953	T7071	T7066	arg2Of	mix,with
R5954	T7071	T7067	arg1Of	mix,the
R5955	T7071	T7068	arg1Of	mix,iQ
R5956	T7071	T7070	arg1Of	mix,Super
R5957	T7071	T7072	arg1Of	mix,(
R5958	T7074	T7072	arg2Of	Laboratories,(
R5959	T7074	T7073	arg1Of	Laboratories,Bio-Rad
R5960	T7075	T7072	arg3Of	),(
R5961	T7076	T7094	arg1Of	and,:
R5962	T7077	T7076	arg2Of	carried,and
R5963	T7077	T7078	arg1Of	carried,out
R5964	T7077	T7079	arg1Of	carried,with
R5965	T7085	T7079	arg2Of	system,with
R5966	T7085	T7080	arg1Of	system,the
R5967	T7085	T7081	arg1Of	system,iCycler
R5968	T7085	T7082	arg1Of	system,iQ
R5969	T7085	T7083	arg1Of	system,Real-Time
R5970	T7085	T7084	arg1Of	system,detection
R5971	T7085	T7086	arg1Of	system,(
R5972	T7085	T7090	arg1Of	system,under
R5973	T7088	T7086	arg2Of	Laboratories,(
R5974	T7088	T7087	arg1Of	Laboratories,Bio-Rad
R5975	T7089	T7086	arg3Of	),(
R5976	T7093	T7090	arg2Of	conditions,under
R5977	T7093	T7091	arg1Of	conditions,the
R5978	T7093	T7092	arg1Of	conditions,following
R5979	T7095	T7096	arg1Of	95°C,","
R5980	T7095	T7098	arg1Of	95°C,;
R5981	T7097	T7096	arg2Of	1 min,","
R5982	T7100	T7099	arg2Of	94°C,(
R5983	T7100	T7101	arg1Of	94°C,","
R5984	T7100	T7103	arg1Of	94°C,;
R5985	T7102	T7101	arg2Of	10 s,","
R5986	T7104	T7103	arg2Of	60°C,;
R5987	T7104	T7105	arg1Of	60°C,","
R5988	T7106	T7105	arg2Of	30 s,","
R5989	T7107	T7099	arg3Of	),(
R5990	T7108	T7098	arg2Of	×40,;
R5991	T7108	T7099	arg1Of	×40,(
R5992	T7109	T7110	arg1Of	Primers,for
R5993	T7109	T7114	arg1Of	Primers,are
R5994	T7109	T7115	arg2Of	Primers,listed
R5995	T7111	T7112	arg1Of	Tat,and
R5996	T7112	T7110	arg2Of	and,for
R5997	T7113	T7112	arg2Of	MIP-1α,and
R5998	T7115	T7114	arg2Of	listed,are
R5999	T7115	T7116	arg1Of	listed,in
R6000	T7119	T7117	arg1Of	Real-time,Supplementary
R6001	T7119	T7118	arg1Of	Real-time,Data.
R6002	T7120	T7116	arg2Of	PCR,in
R6003	T7120	T7119	arg1Of	PCR,Real-time
R6004	T7120	T7121	arg1Of	PCR,of
R6005	T7122	T7121	arg2Of	CSF3,of
R6006	T7124	T7125	arg1Of	LTA,","
R6007	T7125	T7127	arg1Of	",",","
R6008	T7126	T7125	arg2Of	NFKBIA,","
R6009	T7127	T7129	arg1Of	",",","
R6010	T7128	T7127	arg2Of	TLR2,","
R6011	T7129	T7131	arg1Of	",",and
R6012	T7130	T7129	arg2Of	GAPDH,","
R6013	T7131	T7133	arg1Of	and,was
R6014	T7131	T7134	arg2Of	and,performed
R6015	T7132	T7131	arg2Of	ACTB,and
R6016	T7134	T7114	modOf	performed,are
R6017	T7134	T7123	arg1Of	performed,","
R6018	T7134	T7133	arg2Of	performed,was
R6019	T7135	T7134	arg3Of	using,performed
R6020	T7143	T7135	arg2Of	pathway,using
R6021	T7143	T7136	arg1Of	pathway,the
R6022	T7143	T7137	arg1Of	pathway,RT2
R6023	T7143	T7138	arg1Of	pathway,profiler
R6024	T7143	T7139	arg1Of	pathway,PCR
R6025	T7143	T7140	arg1Of	pathway,Array-Human
R6026	T7143	T7141	arg1Of	pathway,NF-κB
R6027	T7143	T7142	arg1Of	pathway,signaling
R6028	T7143	T7144	arg1Of	pathway,(
R6029	T7146	T7144	arg2Of	Sciences,(
R6030	T7146	T7145	arg1Of	Sciences,QIAGEN
R6031	T7146	T7147	arg1Of	Sciences,","
R6032	T7148	T7147	arg2Of	MD,","
R6033	T7148	T7149	arg1Of	MD,","
R6034	T7150	T7149	arg2Of	USA,","
R6035	T7151	T7144	arg3Of	),(
R6036	T7152	T7153	arg1Of	Reactions,were
R6037	T7152	T7154	arg2Of	Reactions,carried
R6038	T7154	T7153	arg2Of	carried,were
R6039	T7154	T7155	arg1Of	carried,out
R6040	T7154	T7156	arg1Of	carried,in
R6041	T7154	T7159	arg1Of	carried,and
R6042	T7157	T7156	arg2Of	triplicate,in
R6043	T7159	T7158	arg1Of	and,","
R6044	T7162	T7160	arg1Of	levels,gene
R6045	T7162	T7161	arg1Of	levels,expression
R6046	T7162	T7163	arg1Of	levels,were
R6047	T7162	T7164	arg2Of	levels,calculated
R6048	T7164	T7159	arg2Of	calculated,and
R6049	T7164	T7163	arg2Of	calculated,were
R6050	T7164	T7166	arg1Of	calculated,to
R6051	T7164	T7170	arg1Of	calculated,as
R6052	T7166	T7165	arg1Of	to,relative
R6053	T7169	T7166	arg2Of	levels,to
R6054	T7169	T7167	arg1Of	levels,GAPDH
R6055	T7169	T7168	arg1Of	levels,mRNA
R6056	T7172	T7170	arg2Of	control,as
R6057	T7172	T7171	arg1Of	control,endogenous
R6058	T7174	T7173	arg1Of	expression,Relative
R6059	T7174	T7175	arg1Of	expression,was
R6060	T7174	T7176	arg2Of	expression,calculated
R6061	T7176	T7175	arg2Of	calculated,was
R6062	T7176	T7177	arg1Of	calculated,as
R6063	T7178	T7177	arg2Of	2,as
R6064	T7178	T7179	arg1Of	2,(
R6065	T7181	T7179	arg2Of	gene,(
R6066	T7181	T7180	arg1Of	gene,Ct
R6067	T7181	T7182	arg1Of	gene,under
R6068	T7184	T7182	arg2Of	GAPDH,under
R6069	T7184	T7183	arg1Of	GAPDH,investigation − Ct
R6070	T7185	T7179	arg3Of	),(
R6779	T8160	T8159	arg1Of	analysis,Statistical
R6780	T8162	T8161	arg1Of	analysis,Statistical
R6781	T8162	T8163	arg1Of	analysis,was
R6782	T8162	T8164	arg2Of	analysis,performed
R6783	T8164	T8163	arg2Of	performed,was
R6784	T8168	T8166	arg1Of	Student,two-tail
R6785	T8168	T8167	arg1Of	Student,unpaired
R6786	T8168	T8169	arg2Of	Student,'s
R6787	T8170	T8164	arg1Of	t-test,performed
R6788	T8170	T8165	arg2Of	t-test,by
R6789	T8170	T8169	arg1Of	t-test,'s
R6790	T8171	T8172	arg1Of	Data,were
R6791	T8171	T8173	arg2Of	Data,reported
R6792	T8173	T8172	arg2Of	reported,were
R6793	T8173	T8174	arg1Of	reported,as
R6794	T8175	T8174	arg2Of	means ± SE,as
R6795	T8176	T8177	arg1Of	Differences,between
R6796	T8176	T8180	arg1Of	Differences,were
R6797	T8176	T8181	arg2Of	Differences,considered
R6798	T8176	T8184	arg1Of	Differences,significant
R6799	T8179	T8177	arg2Of	means,between
R6800	T8179	T8178	arg1Of	means,the
R6801	T8181	T8180	arg2Of	considered,were
R6802	T8181	T8190	arg1Of	considered,(
R6803	T8184	T8181	arg3Of	significant,considered
R6804	T8184	T8182	arg1Of	significant,as
R6805	T8184	T8183	arg1Of	significant,statistically
R6806	T8184	T8185	arg1Of	significant,at
R6807	T8187	T8188	arg1Of	95,%
R6808	T8189	T8185	arg2Of	level,at
R6809	T8189	T8186	arg1Of	level,the
R6810	T8189	T8187	arg1Of	level,95
R6811	T8191	T8190	arg2Of	P ,(
R6812	T8191	T8192	arg1Of	P ,<
R6813	T8192	T8193	arg1Of	<, 0.05
R6814	T8194	T8190	arg3Of	),(
R5852	T6908	T6907	arg2Of	50,(
R5853	T6909	T6907	arg3Of	),(
R5854	T6910	T6911	arg1Of	Details,are
R5855	T6910	T6912	arg2Of	Details,described
R5856	T6912	T6911	arg2Of	described,are
R5857	T6912	T6913	arg1Of	described,in
R5858	T6915	T6913	arg2Of	Data,in
R5859	T6915	T6914	arg1Of	Data,Supplementary

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T4596	23-31	NNP	denotes	Plasmids
T4597	32-35	DT	denotes	The
T4598	36-44	NNS	denotes	plasmids
T4599	45-61	NN	denotes	pcDNA-3xHA-IκB-α
T4600	61-62	,	denotes	,
T4601	63-78	NN	denotes	p3xFLAG-CMV-Tat
T4602	78-79	,	denotes	,
T4603	80-95	NN	denotes	p3xFLAG-CMV-Tat
T4604	96-97	NNP	denotes	C
T4605	97-98	-LRB-	denotes	(
T4606	98-106	CD	denotes	22,25,27
T4607	106-107	-RRB-	denotes	)
T4608	107-108	DT	denotes	A
T4609	108-109	,	denotes	,
T4610	110-125	JJ	denotes	p3xFLAG-CMV-Tat
T4611	126-127	NN	denotes	R
T4612	127-128	-LRB-	denotes	(
T4613	128-145	CD	denotes	49,52,53,55,56,57
T4614	145-146	-RRB-	denotes	)
T4615	146-147	DT	denotes	A
T4616	147-148	,	denotes	,
T4617	149-160	NN	denotes	pGEX-2T-Tat
T4618	160-161	,	denotes	,
T4619	162-173	NN	denotes	pGEX-2T-Tat
T4620	174-175	NNP	denotes	C
T4621	175-176	-LRB-	denotes	(
T4622	176-184	CD	denotes	22,25,27
T4623	184-185	-RRB-	denotes	)
T4624	185-186	DT	denotes	A
T4625	187-190	CC	denotes	and
T4626	191-202	JJ	denotes	pGEX-2T-Tat
T4627	203-204	NN	denotes	R
T4628	204-205	-LRB-	denotes	(
T4629	205-222	CD	denotes	49,52,53,55,56,57
T4630	222-223	-RRB-	denotes	)
T4631	223-224	DT	denotes	A
T4632	225-229	VBD	denotes	were
T4633	230-240	RB	denotes	previously
T4634	241-250	VBN	denotes	described
T4635	251-252	-LRB-	denotes	(
T4636	252-254	CD	denotes	50
T4637	254-255	-RRB-	denotes	)
T4638	255-256	.	denotes	.
T4639	257-260	DT	denotes	The
T4640	261-269	NNS	denotes	plasmids
T4641	270-276	JJ	denotes	pNL4-3
T4642	276-277	.	denotes	.
T4643	277-284	SYM	denotes	Luc.R-E
T4644	284-285	:	denotes	-
T4645	286-289	CC	denotes	and
T4646	290-299	JJ	denotes	pHXB2-env
T4647	300-304	VBD	denotes	were
T4648	305-313	VBN	denotes	obtained
T4649	314-318	IN	denotes	from
T4650	319-322	DT	denotes	the
T4651	323-327	NNP	denotes	AIDS
T4652	328-336	NNP	denotes	Research
T4653	337-338	CC	denotes	&
T4654	339-348	NNP	denotes	Reference
T4655	349-356	NNP	denotes	Reagent
T4656	357-364	NNP	denotes	Program
T4657	364-365	,	denotes	,
T4658	366-374	NNP	denotes	Division
T4659	375-377	IN	denotes	of
T4660	378-382	NNP	denotes	AIDS
T4661	382-383	,	denotes	,
T4662	384-389	NNP	denotes	NIAID
T4663	389-390	,	denotes	,
T4664	391-394	NNP	denotes	NIH
T4665	394-395	,	denotes	,
T4666	396-399	NNP	denotes	USA
T4667	399-400	:	denotes	;
T4668	401-407	NNP	denotes	pκBluc
T4669	408-411	CC	denotes	and
T4670	412-421	JJ	denotes	pSV-β-Gal
T4671	422-426	VBD	denotes	were
T4672	427-436	VBN	denotes	purchased
T4673	437-441	IN	denotes	from
T4674	442-449	NNP	denotes	Promega
T4675	450-451	-LRB-	denotes	(
T4676	451-458	NNP	denotes	Madison
T4677	458-459	,	denotes	,
T4678	460-462	NNP	denotes	WI
T4679	462-463	,	denotes	,
T4680	464-467	NNP	denotes	USA
T4681	467-468	-RRB-	denotes	)
T4682	468-469	.	denotes	.
T4683	470-473	DT	denotes	The
T4684	474-482	NNS	denotes	plasmids
T4685	483-490	NNP	denotes	pRc/CMV
T4686	490-492	CD	denotes	-3
T4687	492-499	NNP	denotes	xHA-p65
T4688	499-500	,	denotes	,
T4689	501-508	NNP	denotes	pRc/CMV
T4690	508-510	CD	denotes	-3
T4691	510-519	NN	denotes	xHA-p65ΔC
T4692	519-520	-LRB-	denotes	(
T4693	520-521	CD	denotes	1
T4694	522-525	CD	denotes	318
T4695	525-526	-RRB-	denotes	)
T4696	526-527	,	denotes	,
T4697	528-535	NNP	denotes	pRc/CMV
T4698	535-537	CD	denotes	-3
T4699	537-546	NNP	denotes	xHA-p65ΔN
T4700	546-547	-LRB-	denotes	(
T4701	547-550	CD	denotes	122
T4702	551-554	CD	denotes	551
T4703	554-555	-RRB-	denotes	)
T4704	555-556	,	denotes	,
T4705	557-572	JJ	denotes	p3xFLAG-CMV-Tat
T4706	573-574	NNP	denotes	T
T4707	574-575	,	denotes	,
T4708	575-576	NNP	denotes	N
T4709	576-577	-LRB-	denotes	(
T4710	577-582	CD	denotes	23,24
T4711	582-583	-RRB-	denotes	)
T4712	583-584	DT	denotes	A
T4713	584-585	,	denotes	,
T4714	586-601	JJ	denotes	p3xFLAG-CMV-Tat
T4715	602-603	NNP	denotes	K
T4716	603-604	-LRB-	denotes	(
T4717	604-609	CD	denotes	50,51
T4718	609-610	-RRB-	denotes	)
T4719	610-611	DT	denotes	A
T4720	611-612	,	denotes	,
T4721	613-624	JJ	denotes	pGEX-2T-Tat
T4722	625-626	NNP	denotes	T
T4723	626-627	,	denotes	,
T4724	627-628	NNP	denotes	N
T4725	628-629	-LRB-	denotes	(
T4726	629-634	CD	denotes	23,24
T4727	634-635	-RRB-	denotes	)
T4728	635-636	DT	denotes	A
T4729	636-637	,	denotes	,
T4730	638-649	JJ	denotes	pGEX-2T-Tat
T4731	650-651	NNP	denotes	K
T4732	651-652	-LRB-	denotes	(
T4733	652-657	CD	denotes	50,51
T4734	657-658	-RRB-	denotes	)
T4735	658-659	DT	denotes	A
T4736	660-663	CC	denotes	and
T4737	664-670	JJ	denotes	pNL4-3
T4738	670-671	.	denotes	.
T4739	671-679	NN	denotes	FLAG-Tat
T4740	679-680	.	denotes	.
T4741	680-683	SYM	denotes	R-E
T4742	683-684	:	denotes	-
T4743	685-689	VBD	denotes	were
T4744	690-699	VBN	denotes	generated
T4745	700-702	IN	denotes	as
T4746	703-712	VBN	denotes	described
T4747	713-715	IN	denotes	in
T4748	716-729	NNP	denotes	Supplementary
T4749	730-734	NNP	denotes	Data
T4750	734-735	.	denotes	.
T5199	737-742	NNS	denotes	Cells
T5200	742-743	,	denotes	,
T5201	744-756	NN	denotes	transfection
T5202	756-757	,	denotes	,
T5203	758-768	NNS	denotes	treatments
T5204	769-772	CC	denotes	and
T5205	773-783	NN	denotes	luciferase
T5206	784-789	NN	denotes	assay
T5207	790-794	NNP	denotes	HeLa
T5208	794-795	,	denotes	,
T5209	796-799	CD	denotes	p50
T5210	799-800	FW	denotes	−
T5211	800-801	FW	denotes	/
T5212	801-802	FW	denotes	−
T5213	802-805	FW	denotes	p65
T5214	805-806	FW	denotes	−
T5215	806-807	FW	denotes	/
T5216	807-808	FW	denotes	−
T5217	809-814	NN	denotes	mouse
T5218	815-824	JJ	denotes	embryonic
T5219	825-836	NNS	denotes	fibroblasts
T5220	837-838	-LRB-	denotes	(
T5221	838-842	NNS	denotes	MEFs
T5222	842-843	-RRB-	denotes	)
T5223	844-845	-LRB-	denotes	(
T5224	845-847	CD	denotes	52
T5225	847-848	-RRB-	denotes	)
T5226	849-852	CC	denotes	and
T5227	853-857	JJ	denotes	293T
T5228	858-863	NNS	denotes	cells
T5229	864-868	VBD	denotes	were
T5230	869-877	VBN	denotes	cultured
T5231	878-880	IN	denotes	in
T5232	881-889	NNP	denotes	Dulbecco
T5233	889-891	POS	denotes	's
T5234	892-900	VBN	denotes	modified
T5235	901-906	NNP	denotes	Eagle
T5236	906-908	POS	denotes	's
T5237	909-915	NN	denotes	medium
T5238	915-916	:	denotes	;
T5239	917-923	NNP	denotes	Jurkat
T5240	923-924	,	denotes	,
T5241	925-929	NNP	denotes	U937
T5242	930-935	NNS	denotes	cells
T5243	936-939	CC	denotes	and
T5244	940-945	JJ	denotes	human
T5245	946-956	JJ	denotes	peripheral
T5246	957-962	NN	denotes	blood
T5247	963-974	NN	denotes	mononuclear
T5248	975-980	NNS	denotes	cells
T5249	981-982	-LRB-	denotes	(
T5250	982-987	NNS	denotes	PBMCs
T5251	987-988	-RRB-	denotes	)
T5252	989-993	VBD	denotes	were
T5253	994-1002	VBN	denotes	cultured
T5254	1003-1005	IN	denotes	in
T5255	1006-1010	NNP	denotes	RPMI
T5256	1011-1015	CD	denotes	1640
T5257	1015-1016	.	denotes	.
T5258	1017-1022	NNS	denotes	PBMCs
T5259	1023-1027	VBD	denotes	were
T5260	1028-1036	VBN	denotes	isolated
T5261	1037-1039	IN	denotes	as
T5262	1040-1050	RB	denotes	previously
T5263	1051-1060	VBN	denotes	described
T5264	1061-1062	-LRB-	denotes	(
T5265	1062-1064	CD	denotes	53
T5266	1064-1065	-RRB-	denotes	)
T5267	1065-1066	.	denotes	.
T5268	1067-1072	NNP	denotes	Media
T5269	1073-1077	VBD	denotes	were
T5270	1078-1090	VBN	denotes	supplemented
T5271	1091-1095	IN	denotes	with
T5272	1096-1098	CD	denotes	10
T5273	1098-1099	NN	denotes	%
T5274	1100-1116	JJ	denotes	heat-inactivated
T5275	1117-1122	JJ	denotes	fetal
T5276	1123-1127	NN	denotes	calf
T5277	1128-1133	NN	denotes	serum
T5278	1134-1137	CC	denotes	and
T5279	1138-1139	CD	denotes	2
T5280	1140-1142	NNP	denotes	mM
T5281	1143-1154	NN	denotes	l-glutamine
T5282	1155-1156	-LRB-	denotes	(
T5283	1156-1161	NNP	denotes	Lonza
T5284	1162-1169	NNP	denotes	Cologne
T5285	1170-1172	NNP	denotes	AG
T5286	1172-1173	,	denotes	,
T5287	1174-1181	NNP	denotes	Germany
T5288	1181-1182	-RRB-	denotes	)
T5289	1182-1183	.	denotes	.
T5290	1184-1188	NNP	denotes	HeLa
T5291	1188-1189	,	denotes	,
T5292	1190-1193	CD	denotes	p50
T5293	1193-1194	FW	denotes	−
T5294	1194-1195	FW	denotes	/
T5295	1195-1196	FW	denotes	−
T5296	1196-1199	FW	denotes	p65
T5297	1199-1200	FW	denotes	−
T5298	1200-1201	FW	denotes	/
T5299	1201-1202	FW	denotes	−
T5300	1203-1207	NNPS	denotes	MEFs
T5301	1208-1211	CC	denotes	and
T5302	1212-1216	NNP	denotes	293T
T5303	1217-1221	VBD	denotes	were
T5304	1222-1233	VBN	denotes	transfected
T5305	1234-1238	IN	denotes	with
T5306	1239-1242	NN	denotes	DNA
T5307	1243-1245	IN	denotes	by
T5308	1246-1251	VBG	denotes	using
T5309	1252-1258	NNP	denotes	FuGENE
T5310	1259-1261	NNP	denotes	HD
T5311	1262-1263	-LRB-	denotes	(
T5312	1263-1268	NNP	denotes	Roche
T5313	1269-1279	NNP	denotes	Diagnostic
T5314	1280-1284	NNP	denotes	GmbH
T5315	1284-1285	,	denotes	,
T5316	1286-1294	NNP	denotes	Mannheim
T5317	1294-1295	,	denotes	,
T5318	1296-1303	NNP	denotes	Germany
T5319	1303-1304	-RRB-	denotes	)
T5320	1304-1305	,	denotes	,
T5321	1306-1315	VBG	denotes	according
T5322	1316-1318	TO	denotes	to
T5323	1319-1322	DT	denotes	the
T5324	1323-1335	NN	denotes	manufacturer
T5325	1335-1337	POS	denotes	's
T5326	1338-1346	NN	denotes	protocol
T5327	1346-1347	:	denotes	;
T5328	1348-1353	JJ	denotes	total
T5329	1354-1357	NNP	denotes	DNA
T5330	1358-1365	NNS	denotes	amounts
T5331	1366-1370	VBD	denotes	were
T5332	1371-1380	VBN	denotes	equalized
T5333	1381-1383	IN	denotes	by
T5334	1384-1396	NN	denotes	transfection
T5335	1397-1399	IN	denotes	of
T5336	1400-1407	NNP	denotes	pRc/CMV
T5337	1408-1413	JJ	denotes	empty
T5338	1414-1420	NN	denotes	vector
T5339	1421-1422	-LRB-	denotes	(
T5340	1422-1432	NNP	denotes	Invitrogen
T5341	1432-1433	,	denotes	,
T5342	1434-1442	NNP	denotes	Carlsbad
T5343	1442-1443	,	denotes	,
T5344	1444-1446	NNP	denotes	CA
T5345	1446-1447	,	denotes	,
T5346	1448-1451	NNP	denotes	USA
T5347	1451-1452	-RRB-	denotes	)
T5348	1452-1453	.	denotes	.
T5349	1454-1457	IN	denotes	For
T5350	1458-1475	NN	denotes	pulse-stimulation
T5351	1475-1476	,	denotes	,
T5352	1477-1481	NNP	denotes	HeLa
T5353	1482-1487	NNS	denotes	cells
T5354	1488-1492	VBD	denotes	were
T5355	1493-1500	VBN	denotes	treated
T5356	1501-1505	IN	denotes	with
T5357	1506-1513	NN	denotes	phorbol
T5358	1514-1526	JJ	denotes	12-myristate
T5359	1527-1537	JJ	denotes	13-acetate
T5360	1538-1539	-LRB-	denotes	(
T5361	1539-1542	NNP	denotes	PMA
T5362	1542-1543	:	denotes	;
T5363	1544-1557	NNP	denotes	Sigma-Aldrich
T5364	1557-1558	,	denotes	,
T5365	1559-1561	NNP	denotes	St
T5366	1562-1567	NNP	denotes	Louis
T5367	1567-1568	,	denotes	,
T5368	1569-1571	NNP	denotes	MO
T5369	1571-1572	,	denotes	,
T5370	1573-1576	NNP	denotes	USA
T5371	1576-1577	-RRB-	denotes	)
T5372	1578-1579	-LRB-	denotes	(
T5373	1579-1581	CD	denotes	20
T5374	1582-1587	NN	denotes	ng/ml
T5375	1587-1588	-RRB-	denotes	)
T5376	1589-1592	IN	denotes	for
T5377	1593-1594	CD	denotes	5
T5378	1595-1598	NN	denotes	min
T5379	1598-1599	,	denotes	,
T5380	1600-1602	CC	denotes	or
T5381	1603-1608	NN	denotes	tumor
T5382	1609-1617	NN	denotes	necrosis
T5383	1618-1626	NN	denotes	factor-α
T5384	1627-1628	-LRB-	denotes	(
T5385	1628-1633	JJ	denotes	TNF-α
T5386	1633-1634	:	denotes	;
T5387	1635-1648	NNP	denotes	Sigma-Aldrich
T5388	1648-1649	-RRB-	denotes	)
T5389	1650-1651	-LRB-	denotes	(
T5390	1651-1653	CD	denotes	20
T5391	1654-1659	NN	denotes	ng/ml
T5392	1659-1660	-RRB-	denotes	)
T5393	1661-1664	IN	denotes	for
T5394	1665-1667	CD	denotes	30
T5395	1668-1671	NN	denotes	min
T5396	1671-1672	,	denotes	,
T5397	1673-1679	VBD	denotes	washed
T5398	1680-1685	RB	denotes	twice
T5399	1686-1688	IN	denotes	in
T5400	1689-1697	JJ	denotes	complete
T5401	1698-1705	NN	denotes	culture
T5402	1706-1712	NN	denotes	medium
T5403	1713-1716	CC	denotes	and
T5404	1717-1721	RB	denotes	then
T5405	1722-1730	VBD	denotes	returned
T5406	1731-1733	TO	denotes	to
T5407	1734-1741	NN	denotes	culture
T5408	1741-1742	.	denotes	.
T5409	1743-1746	IN	denotes	For
T5410	1747-1757	NN	denotes	luciferase
T5411	1758-1764	NNS	denotes	assays
T5412	1764-1765	,	denotes	,
T5413	1766-1775	JJ	denotes	pSV-β-Gal
T5414	1776-1779	VBD	denotes	was
T5415	1780-1794	VBN	denotes	co-transfected
T5416	1795-1799	IN	denotes	with
T5417	1800-1806	NNP	denotes	pκBluc
T5418	1807-1809	TO	denotes	to
T5419	1810-1817	VB	denotes	monitor
T5420	1818-1821	DT	denotes	the
T5421	1822-1834	NN	denotes	transfection
T5422	1835-1845	NN	denotes	efficiency
T5423	1845-1846	.	denotes	.
T5424	1847-1863	JJ	denotes	Forty-eight-hour
T5425	1864-1881	NN	denotes	post-transfection
T5426	1881-1882	,	denotes	,
T5427	1883-1888	NNS	denotes	cells
T5428	1889-1893	VBD	denotes	were
T5429	1894-1899	VBN	denotes	lysed
T5430	1900-1902	IN	denotes	in
T5431	1903-1908	NN	denotes	lysis
T5432	1909-1915	NN	denotes	buffer
T5433	1916-1918	IN	denotes	of
T5434	1919-1923	NNP	denotes	Dual
T5435	1924-1929	NNP	denotes	Light
T5436	1930-1940	NNP	denotes	Luciferase
T5437	1941-1947	NNP	denotes	System
T5438	1948-1949	-LRB-	denotes	(
T5439	1949-1955	NNP	denotes	Tropix
T5440	1955-1956	,	denotes	,
T5441	1957-1964	NNP	denotes	Bedford
T5442	1964-1965	,	denotes	,
T5443	1966-1968	NNP	denotes	MA
T5444	1968-1969	,	denotes	,
T5445	1970-1973	NNP	denotes	USA
T5446	1973-1974	-RRB-	denotes	)
T5447	1975-1978	CC	denotes	and
T5448	1979-1982	DT	denotes	the
T5449	1983-1993	NN	denotes	luciferase
T5450	1994-1997	CC	denotes	and
T5451	1998-2013	JJ	denotes	β-galactosidase
T5452	2014-2024	NNS	denotes	activities
T5453	2025-2029	VBD	denotes	were
T5454	2030-2039	VBN	denotes	evaluated
T5455	2040-2042	IN	denotes	by
T5456	2043-2048	VBG	denotes	using
T5457	2049-2053	NNP	denotes	Dual
T5458	2054-2059	NNP	denotes	Light
T5459	2060-2070	NNP	denotes	Luciferase
T5460	2071-2077	NNP	denotes	System
T5461	2078-2079	-LRB-	denotes	(
T5462	2079-2085	NNP	denotes	Tropix
T5463	2085-2086	-RRB-	denotes	)
T5464	2087-2089	IN	denotes	in
T5465	2090-2091	DT	denotes	a
T5466	2092-2106	NN	denotes	bioluminometer
T5467	2107-2108	-LRB-	denotes	(
T5468	2108-2114	NNP	denotes	Turner
T5469	2115-2124	NNP	denotes	Biosystem
T5470	2124-2125	,	denotes	,
T5471	2126-2135	NNP	denotes	Sunnyvale
T5472	2135-2136	,	denotes	,
T5473	2137-2139	NNP	denotes	CA
T5474	2139-2140	,	denotes	,
T5475	2141-2144	NNP	denotes	USA
T5476	2144-2145	-RRB-	denotes	)
T5477	2145-2146	.	denotes	.
T5478	2147-2150	DT	denotes	The
T5479	2151-2156	NN	denotes	ratio
T5480	2157-2159	IN	denotes	of
T5481	2160-2167	RB	denotes	firefly
T5482	2168-2178	JJ	denotes	luciferase
T5483	2179-2187	NN	denotes	activity
T5484	2188-2190	TO	denotes	to
T5485	2191-2206	JJ	denotes	β-galactosidase
T5486	2207-2215	NN	denotes	activity
T5487	2216-2219	VBD	denotes	was
T5488	2220-2229	VBN	denotes	expressed
T5489	2230-2232	IN	denotes	as
T5490	2233-2241	JJ	denotes	relative
T5491	2242-2247	JJ	denotes	light
T5492	2248-2253	NNS	denotes	units
T5493	2253-2254	.	denotes	.
T5824	2256-2259	NNP	denotes	RNA
T5825	2260-2272	NN	denotes	interference
T5826	2273-2279	NNP	denotes	Jurkat
T5827	2280-2282	CC	denotes	or
T5828	2283-2287	NNP	denotes	U937
T5829	2288-2293	NNS	denotes	cells
T5830	2294-2298	VBD	denotes	were
T5831	2299-2310	VBN	denotes	transfected
T5832	2311-2313	IN	denotes	by
T5833	2314-2329	NN	denotes	electroporation
T5834	2330-2335	VBG	denotes	using
T5835	2336-2337	DT	denotes	a
T5836	2338-2345	NNP	denotes	Bio-Rad
T5837	2346-2355	NN	denotes	apparatus
T5838	2356-2357	-LRB-	denotes	(
T5839	2357-2364	NNP	denotes	Bio-Rad
T5840	2365-2377	NNPS	denotes	Laboratories
T5841	2377-2378	,	denotes	,
T5842	2379-2387	NNP	denotes	Hercules
T5843	2387-2388	,	denotes	,
T5844	2389-2391	NNP	denotes	CA
T5845	2391-2392	,	denotes	,
T5846	2393-2396	NNP	denotes	USA
T5847	2396-2397	-RRB-	denotes	)
T5848	2397-2398	.	denotes	.
T5849	2399-2406	RB	denotes	Briefly
T5850	2406-2407	,	denotes	,
T5851	2408-2416	NNS	denotes	aliquots
T5852	2417-2418	-LRB-	denotes	(
T5853	2418-2419	CD	denotes	5
T5854	2420-2421	CD	denotes	×
T5855	2422-2425	CD	denotes	106
T5856	2426-2431	NNS	denotes	cells
T5857	2431-2432	-RRB-	denotes	)
T5858	2433-2437	VBD	denotes	were
T5859	2438-2447	VBN	denotes	suspended
T5860	2448-2450	IN	denotes	in
T5861	2451-2454	CD	denotes	0.3
T5862	2455-2457	NN	denotes	ml
T5863	2458-2460	IN	denotes	of
T5864	2461-2465	NNP	denotes	RPMI
T5865	2466-2470	CD	denotes	1640
T5866	2471-2483	VBN	denotes	supplemented
T5867	2484-2488	IN	denotes	with
T5868	2489-2491	CD	denotes	20
T5869	2491-2492	NN	denotes	%
T5870	2493-2498	JJ	denotes	fetal
T5871	2499-2503	NN	denotes	calf
T5872	2504-2509	NN	denotes	serum
T5873	2510-2513	CC	denotes	and
T5874	2514-2523	VBN	denotes	subjected
T5875	2524-2526	TO	denotes	to
T5876	2527-2528	DT	denotes	a
T5877	2529-2535	JJ	denotes	double
T5878	2536-2546	JJ	denotes	electrical
T5879	2547-2552	NN	denotes	pulse
T5880	2553-2554	-LRB-	denotes	(
T5881	2554-2558	CD	denotes	0.22
T5882	2559-2560	NNP	denotes	V
T5883	2560-2561	,	denotes	,
T5884	2562-2565	CD	denotes	960
T5885	2566-2568	NN	denotes	µF
T5886	2568-2569	-RRB-	denotes	)
T5887	2570-2572	IN	denotes	in
T5888	2573-2576	DT	denotes	the
T5889	2577-2585	NN	denotes	presence
T5890	2586-2588	IN	denotes	of
T5891	2589-2597	JJ	denotes	annealed
T5892	2598-2603	NNP	denotes	siRNA
T5893	2604-2605	-LRB-	denotes	(
T5894	2605-2608	CD	denotes	200
T5895	2609-2613	NN	denotes	pmol
T5896	2613-2614	-RRB-	denotes	)
T5897	2614-2615	:	denotes	;
T5898	2616-2630	VBD	denotes	electroporated
T5899	2631-2636	NNS	denotes	cells
T5900	2637-2641	VBD	denotes	were
T5901	2642-2648	VBN	denotes	washed
T5902	2649-2652	CC	denotes	and
T5903	2653-2661	VBN	denotes	cultured
T5904	2662-2664	IN	denotes	in
T5905	2665-2673	JJ	denotes	complete
T5906	2674-2680	NN	denotes	medium
T5907	2680-2681	.	denotes	.
T5908	2682-2685	NNP	denotes	RNA
T5909	2686-2698	NN	denotes	interference
T5910	2699-2702	VBD	denotes	was
T5911	2703-2712	VBN	denotes	performed
T5912	2713-2717	IN	denotes	with
T5913	2717-2718	:	denotes	:
T5914	2719-2724	NNP	denotes	siRNA
T5915	2725-2728	NNP	denotes	Tat
T5916	2729-2734	NN	denotes	sense
T5917	2734-2735	,	denotes	,
T5918	2736-2757	NNP	denotes	CUGCUUGUACCAAUUGCUAUU
T5919	2758-2761	CC	denotes	and
T5920	2762-2767	NNP	denotes	siRNA
T5921	2768-2771	NNP	denotes	Tat
T5922	2772-2781	NN	denotes	antisense
T5923	2781-2782	,	denotes	,
T5924	2783-2804	NNP	denotes	UAGCAAUUGGUACAAGCAGUU
T5925	2804-2805	:	denotes	;
T5926	2806-2811	NNP	denotes	siRNA
T5927	2812-2819	NN	denotes	control
T5928	2820-2825	NN	denotes	sense
T5929	2825-2826	,	denotes	,
T5930	2827-2839	NNP	denotes	CUGCUUGUCACA
T5931	2840-2849	NNP	denotes	AUUGCUAUU
T5932	2850-2853	CC	denotes	and
T5933	2854-2859	NNP	denotes	siRNA
T5934	2860-2867	NN	denotes	control
T5935	2868-2877	NN	denotes	antisense
T5936	2877-2878	,	denotes	,
T5937	2879-2900	NNP	denotes	UAGCAAUUGUGACAAGCAGUU
T5938	2900-2901	.	denotes	.
T5939	2902-2905	NNP	denotes	RNA
T5940	2906-2918	NN	denotes	interference
T5941	2919-2921	IN	denotes	of
T5942	2922-2925	CD	denotes	p65
T5943	2926-2929	CC	denotes	and
T5944	2930-2935	NNP	denotes	IκB-α
T5945	2936-2939	VBD	denotes	was
T5946	2940-2949	VBN	denotes	performed
T5947	2950-2954	IN	denotes	with
T5948	2955-2960	NNP	denotes	SMART
T5949	2961-2965	NN	denotes	pool
T5950	2966-2971	NNP	denotes	siRNA
T5951	2972-2975	CD	denotes	p65
T5952	2976-2979	CC	denotes	and
T5953	2980-2985	NNP	denotes	IκB-α
T5954	2986-2987	-LRB-	denotes	(
T5955	2987-2996	NNP	denotes	Dharmacon
T5956	2996-2997	,	denotes	,
T5957	2998-3005	NNP	denotes	Chicago
T5958	3005-3006	,	denotes	,
T5959	3007-3009	NNP	denotes	IL
T5960	3009-3010	,	denotes	,
T5961	3011-3014	NNP	denotes	USA
T5962	3014-3015	-RRB-	denotes	)
T5963	3015-3016	.	denotes	.
T6139	3018-3029	NNP	denotes	Pseudotyped
T6140	3030-3037	NNS	denotes	virions
T6141	3038-3041	CC	denotes	and
T6142	3042-3048	JJ	denotes	single
T6143	3049-3054	NN	denotes	round
T6144	3055-3064	NN	denotes	infection
T6145	3065-3069	JJ	denotes	293T
T6146	3070-3075	NNS	denotes	cells
T6147	3076-3077	-LRB-	denotes	(
T6148	3077-3078	CD	denotes	1
T6149	3079-3080	CD	denotes	×
T6150	3081-3084	CD	denotes	107
T6151	3084-3085	-RRB-	denotes	)
T6152	3086-3090	VBD	denotes	were
T6153	3091-3102	VBN	denotes	transfected
T6154	3103-3107	IN	denotes	with
T6155	3108-3114	JJ	denotes	pNL4-3
T6156	3114-3115	.	denotes	.
T6157	3115-3122	SYM	denotes	Luc.R-E
T6158	3122-3123	:	denotes	-
T6159	3124-3126	CC	denotes	or
T6160	3127-3133	JJ	denotes	pNL4-3
T6161	3133-3134	.	denotes	.
T6162	3134-3142	NN	denotes	FLAG-Tat
T6163	3142-3143	.	denotes	.
T6164	3143-3146	SYM	denotes	R-E
T6165	3146-3147	:	denotes	-
T6166	3148-3149	-LRB-	denotes	(
T6167	3149-3151	CD	denotes	10
T6168	3152-3154	NN	denotes	µg
T6169	3154-3155	-RRB-	denotes	)
T6170	3156-3164	RB	denotes	together
T6171	3165-3169	IN	denotes	with
T6172	3170-3175	NNP	denotes	pHXB2
T6173	3176-3179	NNP	denotes	Env
T6174	3180-3181	-LRB-	denotes	(
T6175	3181-3183	CD	denotes	10
T6176	3184-3186	NN	denotes	µg
T6177	3186-3187	-RRB-	denotes	)
T6178	3187-3188	,	denotes	,
T6179	3189-3192	CC	denotes	and
T6180	3193-3197	JJ	denotes	48-h
T6181	3198-3215	JJ	denotes	post-transfection
T6182	3216-3220	NN	denotes	cell
T6183	3221-3232	NN	denotes	supernatant
T6184	3233-3236	VBD	denotes	was
T6185	3237-3246	VBN	denotes	collected
T6186	3246-3247	.	denotes	.
T6187	3248-3261	JJ	denotes	Enzyme-linked
T6188	3262-3275	JJ	denotes	immunosorbent
T6189	3276-3281	NN	denotes	assay
T6190	3282-3283	-LRB-	denotes	(
T6191	3283-3288	NNP	denotes	ELISA
T6192	3288-3289	-RRB-	denotes	)
T6193	3290-3295	VBG	denotes	using
T6194	3296-3304	JJ	denotes	anti-p24
T6195	3305-3313	NN	denotes	antibody
T6196	3314-3322	VBN	denotes	measured
T6197	3323-3329	NN	denotes	virion
T6198	3330-3343	NN	denotes	concentration
T6199	3343-3344	.	denotes	.
T6200	3345-3350	NNS	denotes	PBMCs
T6201	3350-3351	,	denotes	,
T6202	3352-3358	NNP	denotes	Jurkat
T6203	3359-3361	CC	denotes	or
T6204	3362-3366	NNP	denotes	U937
T6205	3367-3372	NNS	denotes	cells
T6206	3373-3374	-LRB-	denotes	(
T6207	3374-3375	CD	denotes	5
T6208	3376-3377	RB	denotes	×
T6209	3378-3381	CD	denotes	107
T6210	3381-3382	-RRB-	denotes	)
T6211	3383-3387	VBD	denotes	were
T6212	3388-3396	VBN	denotes	infected
T6213	3397-3401	IN	denotes	with
T6214	3402-3406	NNP	denotes	HXB2
T6215	3407-3422	JJ	denotes	Env-pseudotyped
T6216	3423-3430	NNS	denotes	virions
T6217	3431-3432	-LRB-	denotes	(
T6218	3432-3435	CD	denotes	500
T6219	3436-3438	NN	denotes	ng
T6220	3439-3441	IN	denotes	of
T6221	3442-3445	CD	denotes	p24
T6222	3445-3446	-RRB-	denotes	)
T6223	3447-3449	IN	denotes	by
T6224	3450-3463	NN	denotes	spinoculation
T6225	3463-3464	,	denotes	,
T6226	3465-3467	IN	denotes	as
T6227	3468-3478	RB	denotes	previously
T6228	3479-3488	VBN	denotes	described
T6229	3489-3490	-LRB-	denotes	(
T6230	3490-3492	CD	denotes	50
T6231	3492-3493	-RRB-	denotes	)
T6232	3493-3494	.	denotes	.
T6307	3496-3500	NN	denotes	Cell
T6308	3501-3509	NNS	denotes	extracts
T6309	3509-3510	,	denotes	,
T6310	3511-3518	JJ	denotes	western
T6311	3519-3527	VBG	denotes	blotting
T6312	3527-3528	,	denotes	,
T6313	3529-3532	NNP	denotes	IKK
T6314	3533-3541	NN	denotes	activity
T6315	3542-3545	CC	denotes	and
T6316	3546-3551	NNP	denotes	NF-κB
T6317	3552-3555	NNP	denotes	DNA
T6318	3556-3563	JJ	denotes	binding
T6319	3564-3569	NNP	denotes	Total
T6320	3569-3570	,	denotes	,
T6321	3571-3578	JJ	denotes	nuclear
T6322	3579-3582	CC	denotes	and
T6323	3583-3592	JJ	denotes	cytosolic
T6324	3593-3601	NNS	denotes	extracts
T6325	3602-3606	VBD	denotes	were
T6326	3607-3616	VBN	denotes	performed
T6327	3617-3619	IN	denotes	as
T6328	3620-3630	RB	denotes	previously
T6329	3631-3640	VBN	denotes	described
T6330	3641-3642	-LRB-	denotes	(
T6331	3642-3644	CD	denotes	54
T6332	3644-3645	-RRB-	denotes	)
T6333	3645-3646	:	denotes	;
T6334	3647-3654	NNS	denotes	details
T6335	3655-3658	VBP	denotes	are
T6336	3659-3667	VBN	denotes	reported
T6337	3668-3670	IN	denotes	in
T6338	3671-3684	NNP	denotes	Supplementary
T6339	3685-3689	NNP	denotes	Data
T6340	3689-3690	.	denotes	.
T6341	3691-3698	JJ	denotes	Western
T6342	3699-3707	VBG	denotes	blotting
T6343	3708-3716	NN	denotes	analysis
T6344	3717-3720	VBD	denotes	was
T6345	3721-3730	VBN	denotes	performed
T6346	3731-3733	IN	denotes	by
T6347	3734-3746	VBG	denotes	resuspending
T6348	3747-3754	NN	denotes	protein
T6349	3755-3763	NNS	denotes	aliquots
T6350	3764-3766	IN	denotes	in
T6351	3767-3774	VBG	denotes	loading
T6352	3775-3781	NN	denotes	buffer
T6353	3782-3783	-LRB-	denotes	(
T6354	3783-3786	CD	denotes	125
T6355	3787-3789	NNP	denotes	mM
T6356	3790-3794	NNP	denotes	Tris
T6357	3795-3798	NNP	denotes	HCl
T6358	3798-3799	,	denotes	,
T6359	3800-3802	NNP	denotes	pH
T6360	3803-3806	CD	denotes	6.8
T6361	3806-3807	,	denotes	,
T6362	3808-3809	CD	denotes	5
T6363	3809-3810	NN	denotes	%
T6364	3811-3814	NNP	denotes	SDS
T6365	3814-3815	,	denotes	,
T6366	3816-3817	CD	denotes	1
T6367	3817-3818	NN	denotes	%
T6368	3819-3830	NN	denotes	bromophenol
T6369	3831-3835	JJ	denotes	blue
T6370	3835-3836	,	denotes	,
T6371	3837-3839	CD	denotes	10
T6372	3839-3840	NN	denotes	%
T6373	3841-3858	NN	denotes	β-mercaptoethanol
T6374	3858-3859	,	denotes	,
T6375	3860-3862	CD	denotes	25
T6376	3862-3863	NN	denotes	%
T6377	3864-3872	NN	denotes	glycerol
T6378	3872-3873	-RRB-	denotes	)
T6379	3873-3874	,	denotes	,
T6380	3875-3883	VBN	denotes	resolved
T6381	3884-3886	IN	denotes	on
T6382	3887-3889	CD	denotes	12
T6383	3889-3890	NN	denotes	%
T6384	3891-3894	NNP	denotes	SDS
T6385	3895-3899	NNP	denotes	PAGE
T6386	3899-3900	,	denotes	,
T6387	3901-3912	VBD	denotes	transferred
T6388	3913-3915	TO	denotes	to
T6389	3916-3930	VB	denotes	polyvinylidene
T6390	3931-3941	NN	denotes	difluoride
T6391	3942-3950	NN	denotes	membrane
T6392	3951-3952	-LRB-	denotes	(
T6393	3952-3961	NNP	denotes	Millipore
T6394	3961-3962	,	denotes	,
T6395	3963-3970	NNP	denotes	Bedford
T6396	3970-3971	,	denotes	,
T6397	3972-3974	NNP	denotes	MA
T6398	3974-3975	,	denotes	,
T6399	3976-3979	NNP	denotes	USA
T6400	3979-3980	-RRB-	denotes	)
T6401	3981-3984	CC	denotes	and
T6402	3985-3994	VBN	denotes	incubated
T6403	3995-3999	IN	denotes	with
T6404	4000-4007	JJ	denotes	primary
T6405	4008-4018	NNS	denotes	antibodies
T6406	4019-4020	-LRB-	denotes	(
T6407	4020-4026	CD	denotes	1:1000
T6408	4026-4027	-RRB-	denotes	)
T6409	4028-4036	VBN	denotes	followed
T6410	4037-4039	IN	denotes	by
T6411	4040-4050	NN	denotes	incubation
T6412	4051-4055	IN	denotes	with
T6413	4056-4085	JJ	denotes	horseradish-peroxidase-linked
T6414	4086-4091	NN	denotes	mouse
T6415	4092-4094	CC	denotes	or
T6416	4095-4101	NN	denotes	rabbit
T6417	4102-4105	NNP	denotes	IgG
T6418	4106-4107	-LRB-	denotes	(
T6419	4107-4113	CD	denotes	1:2000
T6420	4113-4114	-RRB-	denotes	)
T6421	4115-4116	-LRB-	denotes	(
T6422	4116-4118	NNP	denotes	GE
T6423	4119-4129	NNP	denotes	Healthcare
T6424	4130-4138	NNP	denotes	Amersham
T6425	4138-4139	,	denotes	,
T6426	4140-4146	NNP	denotes	Little
T6427	4147-4155	NNP	denotes	Chalfont
T6428	4155-4156	,	denotes	,
T6429	4157-4172	NNP	denotes	Buckinghamshire
T6430	4172-4173	,	denotes	,
T6431	4174-4176	NNP	denotes	UK
T6432	4176-4177	-RRB-	denotes	)
T6433	4178-4180	IN	denotes	in
T6434	4181-4184	NNP	denotes	PBS
T6435	4185-4195	VBG	denotes	containing
T6436	4196-4197	CD	denotes	5
T6437	4197-4198	NN	denotes	%
T6438	4199-4206	JJ	denotes	non-fat
T6439	4207-4210	JJ	denotes	dry
T6440	4211-4215	NN	denotes	milk
T6441	4216-4217	-LRB-	denotes	(
T6442	4217-4224	NNP	denotes	Bio-Rad
T6443	4225-4237	NNPS	denotes	Laboratories
T6444	4237-4238	-RRB-	denotes	)
T6445	4238-4239	.	denotes	.
T6446	4240-4248	NNPS	denotes	Proteins
T6447	4249-4253	VBD	denotes	were
T6448	4254-4262	VBN	denotes	detected
T6449	4263-4265	IN	denotes	by
T6450	4266-4283	NN	denotes	chemiluminescence
T6451	4284-4289	VBG	denotes	using
T6452	4290-4293	DT	denotes	the
T6453	4294-4297	NNP	denotes	ECL
T6454	4298-4304	NNP	denotes	System
T6455	4305-4306	-LRB-	denotes	(
T6456	4306-4308	NNP	denotes	GE
T6457	4309-4319	NNP	denotes	Healthcare
T6458	4320-4328	NNP	denotes	Amersham
T6459	4328-4329	-RRB-	denotes	)
T6460	4329-4330	.	denotes	.
T6461	4331-4338	JJ	denotes	Primary
T6462	4339-4349	NNS	denotes	antibodies
T6463	4350-4354	VBD	denotes	were
T6464	4355-4364	VBN	denotes	purchased
T6465	4365-4369	IN	denotes	from
T6466	4369-4370	:	denotes	:
T6467	4371-4376	NNP	denotes	Santa
T6468	4377-4381	NNP	denotes	Cruz
T6469	4382-4395	NNP	denotes	Biotechnology
T6470	4395-4396	,	denotes	,
T6471	4397-4402	NNP	denotes	Santa
T6472	4403-4407	NNP	denotes	Cruz
T6473	4407-4408	,	denotes	,
T6474	4409-4411	NNP	denotes	CA
T6475	4411-4412	,	denotes	,
T6476	4413-4416	NNP	denotes	USA
T6477	4417-4418	-LRB-	denotes	(
T6478	4418-4425	JJ	denotes	anti-HA
T6479	4426-4428	NNP	denotes	F7
T6480	4428-4429	,	denotes	,
T6481	4430-4440	NNP	denotes	anti-IκB-α
T6482	4441-4444	NNP	denotes	C15
T6483	4444-4445	,	denotes	,
T6484	4446-4458	JJ	denotes	anti-Histone
T6485	4459-4461	NNP	denotes	H1
T6486	4461-4462	,	denotes	,
T6487	4463-4481	NNP	denotes	anti-Hexokinase-II
T6488	4481-4482	-RRB-	denotes	)
T6489	4482-4483	:	denotes	;
T6490	4484-4497	NNP	denotes	Sigma-Aldrich
T6491	4498-4499	-LRB-	denotes	(
T6492	4499-4508	JJ	denotes	anti-FLAG
T6493	4509-4511	NN	denotes	M2
T6494	4511-4512	,	denotes	,
T6495	4513-4527	JJ	denotes	anti-γ-Tubulin
T6496	4527-4528	-RRB-	denotes	)
T6497	4528-4529	:	denotes	;
T6498	4530-4537	NNP	denotes	Upstate
T6499	4537-4538	,	denotes	,
T6500	4539-4543	NNP	denotes	Lake
T6501	4544-4550	NNP	denotes	Placid
T6502	4550-4551	,	denotes	,
T6503	4552-4554	NNP	denotes	NY
T6504	4554-4555	,	denotes	,
T6505	4556-4559	NNP	denotes	USA
T6506	4560-4561	-LRB-	denotes	(
T6507	4561-4569	JJ	denotes	anti-p65
T6508	4569-4570	-RRB-	denotes	)
T6509	4570-4571	.	denotes	.
T6510	4572-4584	NNP	denotes	Densitometry
T6511	4585-4587	IN	denotes	of
T6512	4588-4594	JJ	denotes	single
T6513	4595-4600	NNS	denotes	bands
T6514	4601-4604	VBD	denotes	was
T6515	4605-4613	VBN	denotes	analysed
T6516	4614-4616	IN	denotes	by
T6517	4617-4623	NNP	denotes	ImageJ
T6518	4624-4632	NN	denotes	software
T6519	4633-4640	NN	denotes	package
T6520	4641-4642	-LRB-	denotes	(
T6521	4642-4645	NNP	denotes	NIH
T6522	4645-4646	,	denotes	,
T6523	4647-4650	NNP	denotes	USA
T6524	4650-4651	-RRB-	denotes	)
T6525	4651-4652	.	denotes	.
T6526	4653-4656	NNP	denotes	IKK
T6527	4657-4665	NN	denotes	activity
T6528	4666-4669	VBD	denotes	was
T6529	4670-4679	VBN	denotes	evaluated
T6530	4680-4682	IN	denotes	in
T6531	4683-4692	JJ	denotes	cytosolic
T6532	4693-4701	NNS	denotes	extracts
T6533	4702-4707	VBG	denotes	using
T6534	4708-4711	DT	denotes	the
T6535	4712-4718	NNP	denotes	HTScan
T6536	4719-4722	NNP	denotes	IKK
T6537	4723-4729	NN	denotes	kinase
T6538	4730-4735	NN	denotes	assay
T6539	4736-4739	NN	denotes	kit
T6540	4740-4741	-LRB-	denotes	(
T6541	4741-4745	NNP	denotes	Cell
T6542	4746-4755	NNP	denotes	Signaling
T6543	4756-4766	NNP	denotes	Technology
T6544	4766-4767	,	denotes	,
T6545	4768-4775	NNP	denotes	Danvers
T6546	4775-4776	,	denotes	,
T6547	4777-4779	NNP	denotes	MA
T6548	4779-4780	,	denotes	,
T6549	4781-4784	NNP	denotes	USA
T6550	4784-4785	-RRB-	denotes	)
T6551	4785-4786	.	denotes	.
T6552	4787-4794	VBG	denotes	Binding
T6553	4795-4797	IN	denotes	of
T6554	4798-4801	CD	denotes	p65
T6555	4801-4802	,	denotes	,
T6556	4803-4806	CD	denotes	p50
T6557	4807-4810	CC	denotes	and
T6558	4811-4819	NNP	denotes	FLAG-Tat
T6559	4820-4822	TO	denotes	to
T6560	4823-4826	DT	denotes	the
T6561	4827-4842	JJ	denotes	double-stranded
T6562	4843-4848	NN	denotes	NF-κB
T6563	4849-4864	NN	denotes	oligonucleotide
T6564	4865-4868	VBD	denotes	was
T6565	4869-4877	VBN	denotes	measured
T6566	4878-4883	VBG	denotes	using
T6567	4884-4889	NNP	denotes	NF-κB
T6568	4890-4895	NNP	denotes	Combo
T6569	4896-4909	NNP	denotes	Transcription
T6570	4910-4916	NNP	denotes	Factor
T6571	4917-4922	NNP	denotes	Assay
T6572	4923-4926	NN	denotes	kit
T6573	4927-4928	-LRB-	denotes	(
T6574	4928-4934	NNP	denotes	Cayman
T6575	4935-4943	NNP	denotes	Chemical
T6576	4944-4951	NNP	denotes	Company
T6577	4951-4952	,	denotes	,
T6578	4953-4956	NNP	denotes	Ann
T6579	4957-4962	NNP	denotes	Arbor
T6580	4962-4963	,	denotes	,
T6581	4964-4966	NNP	denotes	MI
T6582	4966-4967	,	denotes	,
T6583	4968-4971	NNP	denotes	USA
T6584	4971-4972	-RRB-	denotes	)
T6585	4972-4973	.	denotes	.
T6586	4974-4989	NNP	denotes	Electrophoretic
T6587	4990-4998	NNP	denotes	Mobility
T6588	4999-5004	NNP	denotes	Shift
T6589	5005-5010	NNP	denotes	Assay
T6590	5011-5012	-LRB-	denotes	(
T6591	5012-5016	NNP	denotes	EMSA
T6592	5016-5017	-RRB-	denotes	)
T6593	5018-5021	VBD	denotes	was
T6594	5022-5031	VBN	denotes	performed
T6595	5032-5034	IN	denotes	as
T6596	5035-5045	RB	denotes	previously
T6597	5046-5055	VBN	denotes	described
T6598	5056-5057	-LRB-	denotes	(
T6599	5057-5059	CD	denotes	55
T6600	5059-5060	-RRB-	denotes	)
T6601	5060-5061	:	denotes	;
T6602	5062-5069	NNS	denotes	details
T6603	5070-5073	VBP	denotes	are
T6604	5074-5083	VBN	denotes	described
T6605	5084-5086	IN	denotes	in
T6606	5087-5100	NNP	denotes	Supplementary
T6607	5101-5105	NNP	denotes	Data
T6608	5105-5106	.	denotes	.
T6765	5108-5110	IN	denotes	In
T6766	5111-5116	NN	denotes	vitro
T6767	5117-5128	NN	denotes	translation
T6768	5129-5137	NNP	denotes	HA-IκB-α
T6769	5137-5138	,	denotes	,
T6770	5139-5142	CD	denotes	p65
T6771	5143-5146	CC	denotes	and
T6772	5147-5150	NNP	denotes	Tat
T6773	5151-5155	VBD	denotes	were
T6774	5156-5165	VBN	denotes	expressed
T6775	5166-5171	IN	denotes	under
T6776	5172-5175	DT	denotes	the
T6777	5176-5178	CD	denotes	T7
T6778	5179-5187	NN	denotes	promoter
T6779	5188-5191	CC	denotes	and
T6780	5192-5194	IN	denotes	in
T6781	5195-5200	NN	denotes	vitro
T6782	5201-5211	VBN	denotes	translated
T6783	5212-5217	VBG	denotes	using
T6784	5218-5221	DT	denotes	the
T6785	5222-5225	NNP	denotes	TnT
T6786	5226-5231	JJ	denotes	quick
T6787	5232-5239	VBN	denotes	coupled
T6788	5240-5265	NN	denotes	transcription/translation
T6789	5266-5272	NN	denotes	system
T6790	5273-5274	-LRB-	denotes	(
T6791	5274-5281	NNP	denotes	Promega
T6792	5281-5282	-RRB-	denotes	)
T6793	5282-5283	,	denotes	,
T6794	5284-5286	IN	denotes	as
T6795	5287-5297	RB	denotes	previously
T6796	5298-5306	VBN	denotes	reported
T6797	5307-5308	-LRB-	denotes	(
T6798	5308-5310	CD	denotes	50
T6799	5310-5311	-RRB-	denotes	)
T6800	5311-5312	.	denotes	.
T6801	5313-5320	NNS	denotes	Details
T6802	5321-5324	VBP	denotes	are
T6803	5325-5334	VBN	denotes	described
T6804	5335-5337	IN	denotes	in
T6805	5338-5351	NNP	denotes	Supplementary
T6806	5352-5356	NNP	denotes	Data
T6807	5356-5357	.	denotes	.
T6916	5359-5378	NNP	denotes	Immunoprecipitation
T6917	5379-5384	NN	denotes	assay
T6918	5385-5388	CC	denotes	and
T6919	5389-5397	NNP	denotes	GST-pull
T6920	5398-5402	IN	denotes	down
T6921	5403-5422	NNP	denotes	Immunoprecipitation
T6922	5422-5423	,	denotes	,
T6923	5424-5432	NNP	denotes	GST-pull
T6924	5433-5437	RB	denotes	down
T6925	5437-5438	,	denotes	,
T6926	5439-5442	CC	denotes	and
T6927	5443-5453	NN	denotes	production
T6928	5454-5456	IN	denotes	of
T6929	5457-5460	NNP	denotes	GST
T6930	5461-5469	NNS	denotes	proteins
T6931	5470-5472	IN	denotes	in
T6932	5473-5484	NNP	denotes	Escherichia
T6933	5485-5489	NNS	denotes	coli
T6934	5490-5496	VBP	denotes	strain
T6935	5497-5501	CD	denotes	BL21
T6936	5502-5506	VBD	denotes	were
T6937	5507-5516	VBN	denotes	performed
T6938	5517-5519	IN	denotes	as
T6939	5520-5530	RB	denotes	previously
T6940	5531-5539	VBN	denotes	reported
T6941	5540-5541	-LRB-	denotes	(
T6942	5541-5543	CD	denotes	50
T6943	5543-5544	-RRB-	denotes	)
T6944	5544-5545	.	denotes	.
T6945	5546-5553	NNS	denotes	Details
T6946	5554-5557	VBP	denotes	are
T6947	5558-5567	VBN	denotes	described
T6948	5568-5570	IN	denotes	in
T6949	5571-5584	NNP	denotes	Supplementary
T6950	5585-5589	NNP	denotes	Data
T6951	5589-5590	.	denotes	.
T7201	5592-5601	JJ	denotes	Real-time
T7202	5602-5605	NNP	denotes	PCR
T7203	5606-5611	NNP	denotes	Total
T7204	5612-5615	NNP	denotes	RNA
T7205	5616-5619	VBD	denotes	was
T7206	5620-5629	VBN	denotes	extracted
T7207	5630-5634	IN	denotes	from
T7208	5635-5640	NNS	denotes	cells
T7209	5641-5643	IN	denotes	by
T7210	5644-5649	VBG	denotes	using
T7211	5650-5653	DT	denotes	the
T7212	5654-5660	NNP	denotes	TRIzol
T7213	5661-5668	NN	denotes	reagent
T7214	5669-5670	-LRB-	denotes	(
T7215	5670-5680	NNP	denotes	Invitrogen
T7216	5680-5681	-RRB-	denotes	)
T7217	5681-5682	:	denotes	;
T7218	5683-5686	NNP	denotes	RNA
T7219	5687-5695	NNS	denotes	aliquots
T7220	5696-5697	-LRB-	denotes	(
T7221	5697-5700	CD	denotes	200
T7222	5701-5703	NN	denotes	ng
T7223	5703-5704	-RRB-	denotes	)
T7224	5705-5709	VBD	denotes	were
T7225	5710-5717	RB	denotes	reverse
T7226	5718-5729	VBN	denotes	transcribed
T7227	5730-5735	VBG	denotes	using
T7228	5736-5742	NNP	denotes	Random
T7229	5743-5750	NNP	denotes	Examers
T7230	5751-5752	-LRB-	denotes	(
T7231	5752-5757	NNP	denotes	Roche
T7232	5757-5758	-RRB-	denotes	)
T7233	5759-5762	CC	denotes	and
T7234	5763-5774	NNP	denotes	Superscript
T7235	5775-5778	NNP	denotes	III
T7236	5779-5786	VB	denotes	Reverse
T7237	5787-5800	NNP	denotes	Transcriptase
T7238	5801-5802	-LRB-	denotes	(
T7239	5802-5812	NNP	denotes	Invitrogen
T7240	5812-5813	-RRB-	denotes	)
T7241	5813-5814	,	denotes	,
T7242	5815-5824	VBG	denotes	according
T7243	5825-5827	TO	denotes	to
T7244	5828-5831	DT	denotes	the
T7245	5832-5844	NN	denotes	manufacturer
T7246	5844-5846	POS	denotes	's
T7247	5847-5855	NN	denotes	protocol
T7248	5855-5856	.	denotes	.
T7249	5857-5866	JJ	denotes	Real-time
T7250	5867-5870	NNP	denotes	PCR
T7251	5871-5874	VBD	denotes	was
T7252	5875-5884	VBN	denotes	performed
T7253	5885-5889	IN	denotes	with
T7254	5890-5893	DT	denotes	the
T7255	5894-5896	NNP	denotes	iQ
T7256	5897-5902	NNP	denotes	Green
T7257	5903-5908	NNP	denotes	Super
T7258	5909-5912	NN	denotes	mix
T7259	5913-5914	-LRB-	denotes	(
T7260	5914-5921	NNP	denotes	Bio-Rad
T7261	5922-5934	NNPS	denotes	Laboratories
T7262	5934-5935	-RRB-	denotes	)
T7263	5936-5939	CC	denotes	and
T7264	5940-5947	VBD	denotes	carried
T7265	5948-5951	RP	denotes	out
T7266	5952-5956	IN	denotes	with
T7267	5957-5960	DT	denotes	the
T7268	5961-5968	NNP	denotes	iCycler
T7269	5969-5971	NNP	denotes	iQ
T7270	5972-5981	NNP	denotes	Real-Time
T7271	5982-5991	NN	denotes	detection
T7272	5992-5998	NN	denotes	system
T7273	5999-6000	-LRB-	denotes	(
T7274	6000-6007	NNP	denotes	Bio-Rad
T7275	6008-6020	NNPS	denotes	Laboratories
T7276	6020-6021	-RRB-	denotes	)
T7277	6022-6027	IN	denotes	under
T7278	6028-6031	DT	denotes	the
T7279	6032-6041	JJ	denotes	following
T7280	6042-6052	NNS	denotes	conditions
T7281	6052-6053	:	denotes	:
T7282	6054-6056	CD	denotes	95
T7283	6056-6057	NN	denotes	°
T7284	6057-6058	NNP	denotes	C
T7285	6058-6059	,	denotes	,
T7286	6060-6061	CD	denotes	1
T7287	6062-6065	NN	denotes	min
T7288	6065-6066	:	denotes	;
T7289	6067-6068	-LRB-	denotes	(
T7290	6068-6070	CD	denotes	94
T7291	6070-6071	NN	denotes	°
T7292	6071-6072	NNP	denotes	C
T7293	6072-6073	,	denotes	,
T7294	6074-6076	CD	denotes	10
T7295	6077-6078	PRP	denotes	s
T7296	6078-6079	:	denotes	;
T7297	6080-6082	CD	denotes	60
T7298	6082-6083	NN	denotes	°
T7299	6083-6084	NNP	denotes	C
T7300	6084-6085	,	denotes	,
T7301	6086-6088	CD	denotes	30
T7302	6089-6090	PRP	denotes	s
T7303	6090-6091	-RRB-	denotes	)
T7304	6092-6093	RB	denotes	×
T7305	6093-6095	CD	denotes	40
T7306	6095-6096	.	denotes	.
T7307	6097-6104	NNS	denotes	Primers
T7308	6105-6108	IN	denotes	for
T7309	6109-6112	NNP	denotes	Tat
T7310	6113-6116	CC	denotes	and
T7311	6117-6123	NNP	denotes	MIP-1α
T7312	6124-6127	VBP	denotes	are
T7313	6128-6134	VBN	denotes	listed
T7314	6135-6137	IN	denotes	in
T7315	6138-6151	NNP	denotes	Supplementary
T7316	6152-6156	NNP	denotes	Data
T7317	6156-6157	.	denotes	.
T7318	6158-6167	JJ	denotes	Real-time
T7319	6168-6171	NNP	denotes	PCR
T7320	6172-6174	IN	denotes	of
T7321	6175-6179	NNP	denotes	CSF3
T7322	6179-6180	,	denotes	,
T7323	6181-6184	NNP	denotes	LTA
T7324	6184-6185	,	denotes	,
T7325	6186-6192	NNP	denotes	NFKBIA
T7326	6192-6193	,	denotes	,
T7327	6194-6198	NNP	denotes	TLR2
T7328	6198-6199	,	denotes	,
T7329	6200-6205	NNP	denotes	GAPDH
T7330	6206-6209	CC	denotes	and
T7331	6210-6214	NNP	denotes	ACTB
T7332	6215-6218	VBD	denotes	was
T7333	6219-6228	VBN	denotes	performed
T7334	6229-6234	VBG	denotes	using
T7335	6235-6238	DT	denotes	the
T7336	6239-6242	NNP	denotes	RT2
T7337	6243-6251	NN	denotes	profiler
T7338	6252-6255	NNP	denotes	PCR
T7339	6256-6267	NNP	denotes	Array-Human
T7340	6268-6273	NNP	denotes	NF-κB
T7341	6274-6283	VBG	denotes	signaling
T7342	6284-6291	NN	denotes	pathway
T7343	6292-6293	-LRB-	denotes	(
T7344	6293-6299	NNP	denotes	QIAGEN
T7345	6300-6308	NNPS	denotes	Sciences
T7346	6308-6309	,	denotes	,
T7347	6310-6312	NNP	denotes	MD
T7348	6312-6313	,	denotes	,
T7349	6314-6317	NNP	denotes	USA
T7350	6317-6318	-RRB-	denotes	)
T7351	6318-6319	.	denotes	.
T7352	6320-6329	NNS	denotes	Reactions
T7353	6330-6334	VBD	denotes	were
T7354	6335-6342	VBN	denotes	carried
T7355	6343-6346	RP	denotes	out
T7356	6347-6349	IN	denotes	in
T7357	6350-6360	NN	denotes	triplicate
T7358	6360-6361	,	denotes	,
T7359	6362-6365	CC	denotes	and
T7360	6366-6370	NN	denotes	gene
T7361	6371-6381	NN	denotes	expression
T7362	6382-6388	NNS	denotes	levels
T7363	6389-6393	VBD	denotes	were
T7364	6394-6404	VBN	denotes	calculated
T7365	6405-6413	JJ	denotes	relative
T7366	6414-6416	TO	denotes	to
T7367	6417-6422	NNP	denotes	GAPDH
T7368	6423-6427	NNP	denotes	mRNA
T7369	6428-6434	NNS	denotes	levels
T7370	6435-6437	IN	denotes	as
T7371	6438-6448	JJ	denotes	endogenous
T7372	6449-6456	NN	denotes	control
T7373	6456-6457	.	denotes	.
T7374	6458-6466	JJ	denotes	Relative
T7375	6467-6477	NN	denotes	expression
T7376	6478-6481	VBD	denotes	was
T7377	6482-6492	VBN	denotes	calculated
T7378	6493-6495	IN	denotes	as
T7379	6496-6497	CD	denotes	2
T7380	6497-6498	-LRB-	denotes	(
T7381	6498-6500	NN	denotes	Ct
T7382	6501-6505	NN	denotes	gene
T7383	6506-6511	IN	denotes	under
T7384	6512-6525	NN	denotes	investigation
T7385	6526-6527	NN	denotes	−
T7386	6528-6530	NNP	denotes	Ct
T7387	6531-6536	NNP	denotes	GAPDH
T7388	6536-6537	-RRB-	denotes	)
T7389	6537-6538	.	denotes	.
T7546	6540-6549	NNP	denotes	Chromatin
T7547	6550-6569	NN	denotes	immunoprecipitation
T7548	6570-6575	NN	denotes	assay
T7549	6576-6581	NNS	denotes	Cells
T7550	6582-6586	VBD	denotes	were
T7551	6587-6592	VBN	denotes	fixed
T7552	6593-6595	IN	denotes	by
T7553	6596-6602	VBG	denotes	adding
T7554	6603-6615	NN	denotes	formaldehyde
T7555	6616-6617	-LRB-	denotes	(
T7556	6617-6630	NNP	denotes	Sigma-Aldrich
T7589	6785-6788	NNP	denotes	PBS
T7590	6788-6789	,	denotes	,
T7591	6790-6793	CC	denotes	and
T7592	6794-6801	VBD	denotes	scraped
T7593	6801-6802	.	denotes	.
T7594	6803-6808	IN	denotes	After
T7595	6809-6823	NN	denotes	centrifugation
T7596	6823-6824	,	denotes	,
T7597	6825-6830	NNS	denotes	cells
T7598	6831-6835	VBD	denotes	were
T7599	6836-6838	CD	denotes	10
T7600	6839-6842	NN	denotes	min
T7601	6843-6848	VBN	denotes	lysed
T7602	6849-6851	IN	denotes	in
T7603	6852-6857	NN	denotes	lysis
T7604	6858-6864	NN	denotes	buffer
T7605	6865-6866	-LRB-	denotes	(
T7606	6866-6867	CD	denotes	5
T7607	6868-6870	NNP	denotes	mM
T7608	6871-6876	NNP	denotes	PIPES
T7609	6877-6879	NNP	denotes	pH
T7610	6880-6883	CD	denotes	8.0
T7611	6883-6884	,	denotes	,
T7612	6885-6887	CD	denotes	85
T7613	6888-6890	NNP	denotes	mM
T7614	6891-6894	NNP	denotes	KCl
T7615	6894-6895	,	denotes	,
T7616	6896-6899	CD	denotes	0.5
T7617	6899-6900	NN	denotes	%
T7618	6901-6906	NN	denotes	NP-40
T7619	6906-6907	-RRB-	denotes	)
T7620	6908-6920	VBN	denotes	supplemented
T7621	6921-6925	IN	denotes	with
T7622	6926-6927	CD	denotes	1
T7623	6927-6928	CD	denotes	×
T7624	6929-6937	JJ	denotes	Complete
T7625	6938-6946	NNP	denotes	Protease
T7626	6947-6956	NNP	denotes	Inhibitor
T7627	6957-6958	-LRB-	denotes	(
T7628	6958-6963	NNP	denotes	Roche
T7629	6964-6974	NNP	denotes	Diagnostic
T7630	6975-6979	NNP	denotes	GmbH
T7631	6979-6980	-RRB-	denotes	)
T7632	6980-6981	.	denotes	.
T7633	6982-6988	NNP	denotes	Nuclei
T7634	6989-6993	VBD	denotes	were
T7635	6994-7003	VBN	denotes	pelletted
T7636	7004-7005	-LRB-	denotes	(
T7637	7005-7009	CD	denotes	1000
T7638	7010-7011	NN	denotes	×
T7639	7012-7013	NN	denotes	g
T7640	7013-7014	,	denotes	,
T7641	7015-7016	CD	denotes	5
T7642	7017-7020	NN	denotes	min
T7643	7020-7021	-RRB-	denotes	)
T7644	7021-7022	,	denotes	,
T7645	7023-7026	CC	denotes	and
T7646	7027-7038	VBD	denotes	resuspended
T7647	7039-7041	IN	denotes	in
T7648	7042-7052	NN	denotes	sonication
T7649	7053-7059	NN	denotes	buffer
T7650	7060-7061	-LRB-	denotes	(
T7651	7061-7063	CD	denotes	50
T7652	7064-7066	NNP	denotes	mM
T7653	7067-7071	NNP	denotes	Tris
T7654	7072-7075	NNP	denotes	HCl
T7655	7076-7078	NNP	denotes	pH
T7656	7079-7082	CD	denotes	8.0
T7657	7082-7083	,	denotes	,
T7658	7084-7085	CD	denotes	1
T7659	7085-7086	NN	denotes	%
T7660	7087-7090	NNP	denotes	SDS
T7661	7090-7091	,	denotes	,
T7662	7092-7094	CD	denotes	10
T7663	7095-7097	NNP	denotes	mM
T7664	7098-7102	NNP	denotes	EDTA
T7665	7102-7103	-RRB-	denotes	)
T7666	7103-7104	.	denotes	.
T7667	7105-7114	NNP	denotes	Chromatin
T7668	7115-7118	VBD	denotes	was
T7669	7119-7128	VBN	denotes	sonicated
T7670	7129-7134	VBG	denotes	using
T7671	7135-7143	NNP	denotes	Bandelin
T7672	7144-7152	NNPS	denotes	Sonoplus
T7673	7153-7157	NNP	denotes	GM70
T7674	7158-7159	-LRB-	denotes	(
T7675	7159-7167	NNP	denotes	Bandelin
T7676	7168-7178	NNP	denotes	Electronic
T7677	7178-7179	,	denotes	,
T7678	7180-7186	NNP	denotes	Berlin
T7679	7186-7187	,	denotes	,
T7680	7188-7195	NNP	denotes	Germany
T7681	7195-7196	-RRB-	denotes	)
T7682	7196-7197	,	denotes	,
T7683	7198-7209	VBN	denotes	centrifuged
T7684	7210-7211	-LRB-	denotes	(
T7685	7211-7213	CD	denotes	14
T7686	7214-7217	CD	denotes	000
T7687	7218-7219	NN	denotes	×
T7688	7220-7221	NN	denotes	g
T7689	7221-7222	,	denotes	,
T7690	7223-7225	CD	denotes	15
T7691	7226-7229	NN	denotes	min
T7692	7229-7230	-RRB-	denotes	)
T7693	7230-7231	,	denotes	,
T7694	7232-7235	CC	denotes	and
T7695	7236-7247	NN	denotes	supernatant
T7696	7248-7251	VBD	denotes	was
T7697	7252-7259	JJ	denotes	10-fold
T7698	7260-7267	VBN	denotes	diluted
T7699	7268-7270	IN	denotes	in
T7700	7271-7279	NN	denotes	dilution
T7701	7280-7286	NN	denotes	buffer
T7702	7287-7288	-LRB-	denotes	(
T7703	7288-7292	CD	denotes	0.01
T7704	7292-7293	NN	denotes	%
T7705	7294-7297	NNP	denotes	SDS
T7706	7297-7298	,	denotes	,
T7707	7299-7303	CD	denotes	16.7
T7708	7304-7306	NNP	denotes	mM
T7709	7307-7311	NNP	denotes	Tris
T7710	7312-7315	NNP	denotes	HCl
T7711	7316-7318	NNP	denotes	pH
T7712	7319-7322	CD	denotes	8.0
T7713	7322-7323	,	denotes	,
T7714	7324-7327	CD	denotes	1.1
T7715	7327-7328	NN	denotes	%
T7716	7329-7335	NNP	denotes	Triton
T7717	7336-7341	NNP	denotes	X-100
T7718	7341-7342	,	denotes	,
T7719	7343-7346	CD	denotes	167
T7720	7347-7349	NNP	denotes	mM
T7721	7350-7354	NNP	denotes	NaCl
T7722	7354-7355	,	denotes	,
T7723	7356-7359	CD	denotes	1.2
T7724	7360-7362	NNP	denotes	mM
T7725	7363-7367	NNP	denotes	EDTA
T7726	7367-7368	-RRB-	denotes	)
T7727	7368-7369	.	denotes	.
T7728	7370-7377	NNS	denotes	Samples
T7729	7378-7382	VBD	denotes	were
T7730	7383-7394	JJ	denotes	pre-cleared
T7731	7395-7397	IN	denotes	by
T7732	7398-7401	JJ	denotes	3-h
T7733	7402-7412	NN	denotes	incubation
T7734	7413-7417	IN	denotes	with
T7735	7418-7420	CD	denotes	20
T7736	7421-7423	NN	denotes	µl
T7737	7424-7426	IN	denotes	of
T7738	7427-7434	NN	denotes	protein
T7739	7435-7436	NNP	denotes	G
T7740	7437-7444	JJ	denotes	agarose
T7741	7445-7450	NNS	denotes	beads
T7742	7451-7459	VBN	denotes	followed
T7743	7460-7462	IN	denotes	by
T7744	7463-7473	NN	denotes	incubation
T7745	7474-7478	IN	denotes	with
T7746	7479-7489	NNS	denotes	antibodies
T7747	7490-7497	IN	denotes	against
T7748	7498-7501	DT	denotes	the
T7749	7502-7510	JJ	denotes	analysed
T7750	7511-7519	NNS	denotes	proteins
T7751	7519-7520	.	denotes	.
T7752	7521-7528	JJ	denotes	Primary
T7753	7529-7539	NNS	denotes	antibodies
T7754	7540-7544	VBD	denotes	were
T7755	7544-7545	:	denotes	:
T7756	7546-7554	JJ	denotes	anti-p65
T7757	7555-7556	-LRB-	denotes	(
T7758	7556-7562	JJ	denotes	sc-372
T7759	7562-7563	-RRB-	denotes	)
T7760	7563-7564	,	denotes	,
T7761	7565-7574	JJ	denotes	anti-IκBα
T7762	7575-7576	-LRB-	denotes	(
T7763	7576-7582	JJ	denotes	sc-203
T7764	7582-7583	-RRB-	denotes	)
T7765	7584-7587	CC	denotes	and
T7766	7588-7594	NN	denotes	rabbit
T7767	7595-7598	NNP	denotes	IgG
T7768	7599-7600	-LRB-	denotes	(
T7769	7600-7607	JJ	denotes	sc-2027
T7770	7607-7608	-RRB-	denotes	)
T7771	7609-7613	IN	denotes	from
T7772	7614-7619	NNP	denotes	Santa
T7773	7620-7624	NNP	denotes	Cruz
T7774	7625-7638	NNP	denotes	Biotechnology
T7775	7638-7639	:	denotes	;
T7776	7640-7649	NNP	denotes	anti-FLAG
T7777	7650-7652	NNP	denotes	M2
T7778	7653-7657	IN	denotes	from
T7779	7658-7663	NNP	denotes	Sigma
T7780	7664-7671	NNP	denotes	Aldrich
T7781	7671-7672	.	denotes	.
T7782	7673-7693	NNS	denotes	Immunoprecipitations
T7783	7694-7698	VBD	denotes	were
T7784	7699-7706	VBN	denotes	carried
T7785	7707-7710	RP	denotes	out
T7786	7711-7713	IN	denotes	at
T7787	7714-7715	CD	denotes	4
T7788	7715-7716	CD	denotes	°
T7789	7716-7717	NNP	denotes	C
T7790	7718-7727	JJ	denotes	overnight
T7791	7728-7731	CC	denotes	and
T7792	7732-7738	JJ	denotes	immune
T7793	7739-7748	NNS	denotes	complexes
T7794	7749-7753	VBD	denotes	were
T7795	7754-7763	VBN	denotes	collected
T7796	7764-7768	IN	denotes	with
T7797	7769-7776	NN	denotes	protein
T7798	7777-7778	NNP	denotes	G
T7799	7779-7786	JJ	denotes	agarose
T7800	7787-7792	NNS	denotes	beads
T7801	7792-7793	,	denotes	,
T7802	7794-7800	VBD	denotes	washed
T7803	7801-7805	CD	denotes	five
T7804	7806-7811	NNS	denotes	times
T7805	7812-7816	IN	denotes	with
T7806	7817-7820	JJ	denotes	low
T7807	7821-7825	NN	denotes	salt
T7808	7826-7832	NN	denotes	buffer
T7809	7833-7834	-LRB-	denotes	(
T7810	7834-7836	CD	denotes	20
T7811	7837-7839	NNP	denotes	mM
T7812	7840-7844	NNP	denotes	Tris
T7813	7845-7848	NNP	denotes	HCl
T7814	7849-7851	NNP	denotes	pH
T7815	7852-7855	CD	denotes	8.0
T7816	7855-7856	,	denotes	,
T7817	7857-7860	CD	denotes	0.1
T7818	7860-7861	NN	denotes	%
T7819	7862-7865	NNP	denotes	SDS
T7820	7865-7866	,	denotes	,
T7821	7867-7868	CD	denotes	1
T7822	7868-7869	NN	denotes	%
T7823	7870-7876	NNP	denotes	Triton
T7824	7877-7882	NNP	denotes	X-100
T7825	7882-7883	,	denotes	,
T7826	7884-7885	CD	denotes	2
T7827	7886-7888	NNP	denotes	mM
T7828	7889-7893	NNP	denotes	EDTA
T7829	7893-7894	,	denotes	,
T7830	7895-7898	CD	denotes	150
T7831	7899-7901	NNP	denotes	mM
T7832	7902-7906	NNP	denotes	NaCl
T7833	7906-7907	-RRB-	denotes	)
T7834	7907-7908	,	denotes	,
T7835	7909-7913	CD	denotes	four
T7836	7914-7919	NNS	denotes	times
T7837	7920-7924	IN	denotes	with
T7838	7925-7929	JJ	denotes	high
T7839	7930-7934	NN	denotes	salt
T7840	7935-7941	NN	denotes	buffer
T7841	7942-7943	-LRB-	denotes	(
T7842	7943-7945	CD	denotes	20
T7843	7946-7948	NNP	denotes	mM
T7844	7949-7953	NNP	denotes	Tris
T7845	7954-7957	NNP	denotes	HCl
T7846	7958-7960	NNP	denotes	pH
T7847	7961-7964	CD	denotes	8.0
T7848	7964-7965	,	denotes	,
T7849	7966-7969	CD	denotes	0.1
T7850	7969-7970	NN	denotes	%
T7851	7971-7974	NNP	denotes	SDS
T7852	7974-7975	,	denotes	,
T7853	7976-7977	CD	denotes	1
T7854	7977-7978	NN	denotes	%
T7855	7979-7985	NNP	denotes	Triton
T7856	7986-7991	NNP	denotes	X-100
T7857	7991-7992	,	denotes	,
T7858	7993-7994	CD	denotes	2
T7859	7995-7997	NNP	denotes	mM
T7860	7998-8002	NNP	denotes	EDTA
T7861	8002-8003	,	denotes	,
T7862	8004-8007	CD	denotes	500
T7863	8008-8010	NNP	denotes	mM
T7864	8011-8015	NNP	denotes	NaCl
T7865	8015-8016	-RRB-	denotes	)
T7866	8016-8017	,	denotes	,
T7867	8018-8022	RB	denotes	once
T7868	8023-8027	IN	denotes	with
T7869	8028-8030	NNP	denotes	TE
T7870	8031-8037	NN	denotes	buffer
T7871	8038-8039	-LRB-	denotes	(
T7872	8039-8041	CD	denotes	10
T7873	8042-8044	NNP	denotes	mM
T7874	8045-8049	NNP	denotes	Tris
T7875	8050-8053	NNP	denotes	HCl
T7876	8054-8056	NNP	denotes	pH
T7877	8057-8060	CD	denotes	8.0
T7878	8060-8061	,	denotes	,
T7879	8062-8063	CD	denotes	1
T7915	8242-8245	NN	denotes	kit
T7916	8246-8247	-LRB-	denotes	(
T7917	8247-8253	NNP	denotes	QIAGEN
T7918	8253-8254	-RRB-	denotes	)
T7919	8255-8258	CC	denotes	and
T7920	8259-8265	VBD	denotes	eluted
T7921	8266-8268	IN	denotes	in
T7922	8269-8271	CD	denotes	50
T7923	8272-8274	NN	denotes	µl
T7924	8275-8282	JJ	denotes	sterile
T7925	8283-8292	JJ	denotes	distilled
T7926	8293-8298	NN	denotes	water
T7927	8298-8299	.	denotes	.
T7928	8300-8308	JJ	denotes	Specific
T7929	8309-8319	NN	denotes	enrichment
T7930	8320-8322	IN	denotes	in
T7931	8323-8328	JJ	denotes	NF-κB
T7932	8329-8337	NN	denotes	enhancer
T7933	8338-8347	NNS	denotes	sequences
T7934	8348-8351	VBD	denotes	was
T7935	8352-8360	VBN	denotes	measured
T7936	8361-8363	IN	denotes	by
T7937	8364-8373	JJ	denotes	real-time
T7938	8374-8377	NNP	denotes	PCR
T7939	8378-8380	IN	denotes	of
T7940	8381-8390	NN	denotes	chromatin
T7941	8391-8410	NN	denotes	immunoprecipitation
T7942	8411-8412	-LRB-	denotes	(
T7943	8412-8416	NNP	denotes	ChIP
T7944	8416-8417	-RRB-	denotes	)
T7956	8480-8484	VBD	denotes	were
T7957	8485-8492	VBN	denotes	carried
T7958	8493-8496	RP	denotes	out
T7959	8497-8501	IN	denotes	with
T7960	8502-8505	DT	denotes	the
T7961	8506-8513	NNP	denotes	iCycler
T7962	8514-8516	NNP	denotes	iQ
T7963	8517-8526	NNP	denotes	Real-Time
T7964	8527-8536	NN	denotes	detection
T7965	8537-8543	NN	denotes	system
T7966	8544-8545	-LRB-	denotes	(
T7967	8545-8552	NNP	denotes	Bio-Rad
T7968	8553-8565	NNPS	denotes	Laboratories
T7969	8565-8566	-RRB-	denotes	)
T7970	8567-8572	VBG	denotes	using
T7971	8573-8576	DT	denotes	the
T7972	8577-8586	JJ	denotes	following
T7973	8587-8597	NNS	denotes	conditions
T7974	8597-8598	:	denotes	:
T7975	8599-8601	CD	denotes	95
T7976	8601-8602	NN	denotes	°
T7977	8602-8603	NNP	denotes	C
T7978	8603-8604	,	denotes	,
T7979	8605-8606	CD	denotes	1
T7980	8607-8610	NN	denotes	min
T7981	8610-8611	:	denotes	;
T7982	8612-8613	-LRB-	denotes	(
T7983	8613-8615	CD	denotes	94
T7984	8615-8616	NN	denotes	°
T7985	8616-8617	NNP	denotes	C
T7986	8617-8618	,	denotes	,
T7987	8619-8621	CD	denotes	10
T7988	8622-8623	PRP	denotes	s
T7989	8623-8624	:	denotes	;
T7990	8625-8627	CD	denotes	60
T7991	8627-8628	NN	denotes	°
T7992	8628-8629	NNP	denotes	C
T7993	8629-8630	,	denotes	,
T7994	8631-8633	CD	denotes	30
T7995	8634-8635	PRP	denotes	s
T7996	8635-8636	-RRB-	denotes	)
T7997	8637-8638	RB	denotes	×
T7998	8638-8640	CD	denotes	40
T7999	8640-8641	.	denotes	.
T8000	8642-8649	NNS	denotes	Primers
T8001	8650-8654	VBD	denotes	used
T8002	8655-8658	IN	denotes	for
T8003	8659-8665	NNP	denotes	MIP-1α
T8004	8665-8666	,	denotes	,
T8005	8667-8672	NNP	denotes	GAPDH
T8006	8673-8676	CC	denotes	and
T8007	8677-8681	NNP	denotes	ACTB
T8008	8682-8685	VBP	denotes	are
T8009	8686-8692	VBN	denotes	listed
T8010	8693-8695	IN	denotes	in
T8011	8696-8709	NNP	denotes	Supplementary
T8012	8710-8714	NNP	denotes	Data
T8013	8714-8715	.	denotes	.
T8014	8716-8725	JJ	denotes	Real-time
T8015	8726-8729	NNP	denotes	PCR
T8016	8730-8732	IN	denotes	of
T8017	8733-8737	NNP	denotes	CSF3
T8018	8737-8738	,	denotes	,
T8019	8739-8742	NNP	denotes	LTA
T8020	8742-8743	,	denotes	,
T8021	8744-8750	NNP	denotes	NFKBIA
T8022	8751-8754	CC	denotes	and
T8023	8755-8759	CD	denotes	TLR2
T8024	8760-8764	VBD	denotes	were
T8025	8765-8774	VBN	denotes	performed
T8026	8775-8780	VBG	denotes	using
T8027	8781-8784	DT	denotes	the
T8028	8785-8791	NNP	denotes	Custom
T8029	8792-8796	NNP	denotes	ChIP
T8030	8797-8802	NN	denotes	array
T8031	8803-8804	-LRB-	denotes	(
T8032	8804-8810	NNP	denotes	QIAGEN
T8033	8810-8811	-RRB-	denotes	)
T8034	8811-8812	.	denotes	.
T8035	8813-8816	IN	denotes	For
T8036	8817-8821	DT	denotes	each
T8037	8822-8828	NN	denotes	sample
T8038	8828-8829	,	denotes	,
T8039	8830-8836	NNS	denotes	values
T8040	8837-8841	VBD	denotes	were
T8041	8842-8852	VBN	denotes	normalized
T8042	8853-8855	TO	denotes	to
T8043	8856-8861	NN	denotes	input
T8044	8862-8865	NN	denotes	DNA
T8045	8866-8869	CC	denotes	and
T8046	8870-8878	VBD	denotes	reported
T8047	8879-8881	IN	denotes	as
T8048	8882-8883	NN	denotes	%
T8049	8884-8886	IN	denotes	of
T8050	8887-8892	NN	denotes	input
T8051	8893-8897	IN	denotes	over
T8052	8898-8901	DT	denotes	the
T8053	8902-8908	NN	denotes	rabbit
T8054	8909-8912	NNP	denotes	IgG
T8055	8913-8920	NN	denotes	control
T8056	8920-8921	.	denotes	.
T8195	8923-8934	NNP	denotes	Statistical
T8196	8935-8943	NN	denotes	analysis
T8197	8944-8955	NNP	denotes	Statistical
T8198	8956-8964	NN	denotes	analysis
T8199	8965-8968	VBD	denotes	was
T8200	8969-8978	VBN	denotes	performed
T8201	8979-8981	IN	denotes	by
T8202	8982-8990	JJ	denotes	two-tail
T8203	8991-8999	JJ	denotes	unpaired
T8204	9000-9007	NN	denotes	Student
T8205	9007-9009	POS	denotes	's
T8206	9010-9016	JJS	denotes	t-test
T8207	9016-9017	.	denotes	.
T8208	9018-9022	NNS	denotes	Data
T8209	9023-9027	VBD	denotes	were
T8210	9028-9036	VBN	denotes	reported
T8211	9037-9039	IN	denotes	as
T8212	9040-9045	NNS	denotes	means
T8213	9046-9047	VBP	denotes	±
T8214	9048-9050	NNP	denotes	SE
T8215	9050-9051	.	denotes	.
T8216	9052-9063	NNS	denotes	Differences
T8217	9064-9071	IN	denotes	between
T8218	9072-9075	DT	denotes	the
T8219	9076-9081	NNS	denotes	means
T8220	9082-9086	VBD	denotes	were
T8221	9087-9097	VBN	denotes	considered
T8222	9098-9100	IN	denotes	as
T8223	9101-9114	RB	denotes	statistically
T8224	9115-9126	JJ	denotes	significant
T8225	9127-9129	IN	denotes	at
T8226	9130-9133	DT	denotes	the
T8227	9134-9136	CD	denotes	95
T8228	9136-9137	NN	denotes	%
T8229	9138-9143	NN	denotes	level
T8230	9144-9145	-LRB-	denotes	(
T8231	9145-9146	NNP	denotes	P
T8232	9147-9148	CD	denotes	<
T8233	9149-9153	CD	denotes	0.05
T8234	9153-9154	-RRB-	denotes	)
T8235	9154-9155	.	denotes	.
T7557	6630-6631	-RRB-	denotes	)
T7558	6632-6634	IN	denotes	at
T7559	6635-6638	DT	denotes	the
T7560	6639-6644	JJ	denotes	final
T7561	6645-6658	NN	denotes	concentration
T7562	6659-6661	IN	denotes	of
T7563	6662-6663	CD	denotes	1
T7564	6663-6664	NN	denotes	%
T7565	6664-6665	.	denotes	.
T7566	6666-6671	IN	denotes	After
T7567	6672-6674	CD	denotes	10
T7568	6675-6678	NN	denotes	min
T7569	6678-6679	,	denotes	,
T7570	6680-6688	JJ	denotes	ice-cold
T7571	6689-6692	NNP	denotes	PBS
T7572	6693-6697	CC	denotes	plus
T7573	6698-6703	CD	denotes	0.125
T7574	6704-6705	NNP	denotes	M
T7575	6706-6713	NN	denotes	glycine
T7576	6714-6717	VBD	denotes	was
T7577	6718-6723	VBN	denotes	added
T7578	6723-6724	,	denotes	,
T7579	6725-6728	CC	denotes	and
T7580	6729-6735	NNS	denotes	plates
T7581	6736-6740	VBD	denotes	were
T7582	6741-6752	VBN	denotes	transferred
T7583	6753-6755	IN	denotes	on
T7584	6756-6759	NN	denotes	ice
T7585	6759-6760	,	denotes	,
T7586	6761-6767	VBD	denotes	washed
T7587	6768-6779	RB	denotes	extensively
T7588	6780-6784	IN	denotes	with
T7880	8064-8066	NNP	denotes	mM
T7881	8067-8071	NNP	denotes	EDTA
T7882	8071-8072	-RRB-	denotes	)
T7883	8072-8073	,	denotes	,
T7884	8074-8077	CC	denotes	and
T7885	8078-8087	VBN	denotes	extracted
T7886	8088-8090	IN	denotes	in
T7887	8091-8093	NNP	denotes	TE
T7888	8094-8100	NN	denotes	buffer
T7889	8101-8111	VBG	denotes	containing
T7890	8112-8113	CD	denotes	2
T7891	8113-8114	NN	denotes	%
T7892	8115-8118	NNP	denotes	SDS
T7893	8118-8119	.	denotes	.
T7894	8120-8127	NN	denotes	Protein
T7895	8128-8131	NNP	denotes	DNA
T7896	8132-8143	NNS	denotes	cross-links
T7897	8144-8148	VBD	denotes	were
T7898	8149-8157	VBN	denotes	reverted
T7899	8158-8160	IN	denotes	by
T7900	8161-8168	NN	denotes	heating
T7901	8169-8171	IN	denotes	at
T7902	8172-8174	CD	denotes	65
T7903	8174-8175	CD	denotes	°
T7904	8175-8176	NNP	denotes	C
T7905	8177-8186	JJ	denotes	overnight
T7906	8186-8187	.	denotes	.
T7907	8188-8191	NNP	denotes	DNA
T7908	8192-8195	VBD	denotes	was
T7909	8196-8203	JJ	denotes	further
T7910	8204-8212	VBN	denotes	purified
T7911	8213-8215	IN	denotes	by
T7912	8216-8224	NNP	denotes	QIAquick
T7913	8225-8228	NNP	denotes	PCR
T7914	8229-8241	NN	denotes	purification
T7945	8418-8425	VBZ	denotes	eluates
T7946	8426-8431	VBG	denotes	using
T7947	8432-8436	NNP	denotes	SYBR
T7948	8437-8444	NNP	denotes	GreenER
T7949	8445-8451	NNP	denotes	Master
T7950	8452-8455	NNP	denotes	Mix
T7951	8456-8457	-LRB-	denotes	(
T7952	8457-8467	NNP	denotes	Invitrogen
T7953	8467-8468	-RRB-	denotes	)
T7954	8468-8469	.	denotes	.
T7955	8470-8479	NNS	denotes	Reactions
R4150	T4596	T4596	ROOT	Plasmids,Plasmids
R4151	T4597	T4599	det	The,pcDNA-3xHA-IκB-α
R4152	T4598	T4599	compound	plasmids,pcDNA-3xHA-IκB-α
R4153	T4599	T4596	appos	pcDNA-3xHA-IκB-α,Plasmids
R4154	T4600	T4599	punct	",",pcDNA-3xHA-IκB-α
R4155	T4601	T4599	conj	p3xFLAG-CMV-Tat,pcDNA-3xHA-IκB-α
R4156	T4602	T4601	punct	",",p3xFLAG-CMV-Tat
R4157	T4603	T4604	compound	p3xFLAG-CMV-Tat,C
R4158	T4604	T4599	appos	C,pcDNA-3xHA-IκB-α
R4159	T4605	T4604	punct	(,C
R4160	T4606	T4604	appos	"22,25,27",C
R4161	T4607	T4604	punct	),C
R4162	T4608	T4604	appos	A,C
R4163	T4609	T4611	punct	",",R
R4164	T4610	T4611	amod	p3xFLAG-CMV-Tat,R
R4165	T4611	T4604	appos	R,C
R4166	T4612	T4611	punct	(,R
R4167	T4613	T4611	appos	"49,52,53,55,56,57",R
R4168	T4614	T4611	punct	),R
R4169	T4615	T4620	det	A,C
R4170	T4616	T4617	punct	",",pGEX-2T-Tat
R4171	T4617	T4620	nmod	pGEX-2T-Tat,C
R4172	T4618	T4620	punct	",",C
R4173	T4619	T4620	compound	pGEX-2T-Tat,C
R4174	T4620	T4620	ROOT	C,C
R4175	T4621	T4620	punct	(,C
R4176	T4622	T4620	appos	"22,25,27",C
R4177	T4623	T4620	punct	),C
R4178	T4624	T4624	ROOT	A,A
R4179	T4625	T4624	cc	and,A
R4180	T4626	T4627	amod	pGEX-2T-Tat,R
R4181	T4627	T4624	conj	R,A
R4182	T4628	T4627	punct	(,R
R4183	T4629	T4627	appos	"49,52,53,55,56,57",R
R4184	T4630	T4627	punct	),R
R4185	T4631	T4634	nsubjpass	A,described
R4186	T4632	T4634	auxpass	were,described
R4187	T4633	T4634	advmod	previously,described
R4188	T4634	T4634	ROOT	described,described
R4189	T4635	T4634	punct	(,described
R4190	T4636	T4634	npadvmod	50,described
R4191	T4637	T4634	punct	),described
R4192	T4638	T4634	punct	.,described
R4193	T4639	T4641	det	The,pNL4-3
R4194	T4640	T4641	compound	plasmids,pNL4-3
R4195	T4641	T4648	nsubjpass	pNL4-3,obtained
R4196	T4642	T4641	punct	.,pNL4-3
R4197	T4643	T4648	punct	Luc.R-E,obtained
R4198	T4644	T4643	punct	-,Luc.R-E
R4199	T4645	T4643	cc	and,Luc.R-E
R4200	T4646	T4643	conj	pHXB2-env,Luc.R-E
R4201	T4647	T4648	auxpass	were,obtained
R4202	T4648	T4672	ccomp	obtained,purchased
R4203	T4649	T4648	prep	from,obtained
R4204	T4650	T4656	det	the,Program
R4205	T4651	T4652	compound	AIDS,Research
R4206	T4652	T4656	nmod	Research,Program
R4207	T4653	T4652	cc	&,Research
R4208	T4654	T4652	conj	Reference,Research
R4209	T4655	T4656	compound	Reagent,Program
R4210	T4656	T4649	pobj	Program,from
R4212	T4658	T4656	conj	Division,Program
R4213	T4659	T4658	prep	of,Division
R4214	T4660	T4659	pobj	AIDS,of
R4215	T4661	T4658	punct	",",Division
R4216	T4662	T4658	conj	NIAID,Division
R4217	T4663	T4662	punct	",",NIAID
R4224	T4670	T4668	conj	pSV-β-Gal,pκBluc
R4225	T4671	T4672	auxpass	were,purchased
R4226	T4672	T4672	ROOT	purchased,purchased
R4227	T4673	T4672	prep	from,purchased
R4228	T4674	T4673	pobj	Promega,from
R4229	T4675	T4676	punct	(,Madison
R4230	T4676	T4674	appos	Madison,Promega
R4231	T4677	T4676	punct	",",Madison
R4232	T4678	T4676	conj	WI,Madison
R4233	T4679	T4678	punct	",",WI
R4234	T4680	T4678	npadvmod	USA,WI
R4235	T4681	T4678	punct	),WI
R4236	T4682	T4672	punct	.,purchased
R4237	T4683	T4687	det	The,xHA-p65
R4238	T4684	T4685	compound	plasmids,pRc/CMV
R4239	T4685	T4687	nmod	pRc/CMV,xHA-p65
R4240	T4686	T4687	nummod	-3,xHA-p65
R4241	T4687	T4687	ROOT	xHA-p65,xHA-p65
R4242	T4688	T4687	punct	",",xHA-p65
R4243	T4689	T4687	appos	pRc/CMV,xHA-p65
R4244	T4690	T4691	nummod	-3,xHA-p65ΔC
R4245	T4691	T4687	appos	xHA-p65ΔC,xHA-p65
R4246	T4692	T4693	punct	(,1
R4247	T4693	T4699	nummod	1,xHA-p65ΔN
R4248	T4694	T4693	appos	318,1
R4249	T4695	T4693	punct	),1
R4250	T4696	T4693	punct	",",1
R4251	T4697	T4699	nmod	pRc/CMV,xHA-p65ΔN
R4252	T4698	T4699	nummod	-3,xHA-p65ΔN
R4253	T4699	T4691	appos	xHA-p65ΔN,xHA-p65ΔC
R4254	T4700	T4699	punct	(,xHA-p65ΔN
R4255	T4701	T4699	parataxis	122,xHA-p65ΔN
R4256	T4702	T4701	prep	551,122
R4257	T4703	T4701	punct	),122
R4258	T4704	T4706	punct	",",T
R4259	T4705	T4706	compound	p3xFLAG-CMV-Tat,T
R4260	T4706	T4699	conj	T,xHA-p65ΔN
R4261	T4707	T4706	punct	",",T
R4262	T4708	T4706	npadvmod	N,T
R4263	T4709	T4710	punct	(,"23,24"
R4264	T4710	T4708	parataxis	"23,24",N
R4265	T4711	T4710	punct	),"23,24"
R4266	T4712	T4715	det	A,K
R4267	T4713	T4715	punct	",",K
R4268	T4714	T4715	compound	p3xFLAG-CMV-Tat,K
R4269	T4715	T4715	ROOT	K,K
R4270	T4716	T4715	punct	(,K
R4271	T4717	T4715	appos	"50,51",K
R4272	T4718	T4715	punct	),K
R4273	T4719	T4722	det	A,T
R4274	T4720	T4722	punct	",",T
R4275	T4721	T4722	compound	pGEX-2T-Tat,T
R4276	T4722	T4722	ROOT	T,T
R4277	T4723	T4722	punct	",",T
R4278	T4724	T4722	conj	N,T
R4279	T4725	T4724	punct	(,N
R4280	T4726	T4724	appos	"23,24",N
R4281	T4727	T4724	punct	),N
R4282	T4728	T4728	ROOT	A,A
R4283	T4729	T4735	punct	",",A
R4284	T4730	T4731	amod	pGEX-2T-Tat,K
R4285	T4731	T4735	nmod	K,A
R4286	T4732	T4731	punct	(,K
R4287	T4733	T4731	appos	"50,51",K
R4288	T4734	T4735	punct	),A
R4289	T4735	T4728	det	A,A
R4290	T4736	T4735	cc	and,A
R4291	T4737	T4735	conj	pNL4-3,A
R4292	T4738	T4737	punct	.,pNL4-3
R4293	T4739	T4739	ROOT	FLAG-Tat,FLAG-Tat
R4294	T4740	T4739	punct	.,FLAG-Tat
R4295	T4741	T4744	nsubjpass	R-E,generated
R4296	T4742	T4744	punct	-,generated
R4297	T4743	T4744	auxpass	were,generated
R4298	T4744	T4744	ROOT	generated,generated
R4299	T4745	T4746	mark	as,described
R4300	T4746	T4744	advcl	described,generated
R4301	T4747	T4746	prep	in,described
R4302	T4748	T4749	compound	Supplementary,Data
R4303	T4749	T4747	pobj	Data,in
R4304	T4750	T4744	punct	.,generated
R4581	T5199	T5207	nmod	Cells,HeLa
R4582	T5200	T5199	punct	",",Cells
R4583	T5201	T5199	conj	transfection,Cells
R4584	T5202	T5201	punct	",",transfection
R4585	T5203	T5201	conj	treatments,transfection
R4586	T5204	T5203	cc	and,treatments
R4587	T5205	T5206	compound	luciferase,assay
R4588	T5206	T5203	conj	assay,treatments
R4589	T5207	T5230	nsubjpass	HeLa,cultured
R4590	T5208	T5207	punct	",",HeLa
R4591	T5209	T5219	nummod	p50,fibroblasts
R4592	T5210	T5219	nmod	−,fibroblasts
R4593	T5211	T5219	nmod	/,fibroblasts
R4594	T5212	T5219	nmod	−,fibroblasts
R4595	T5213	T5219	nmod	p65,fibroblasts
R4596	T5214	T5219	nmod	−,fibroblasts
R4597	T5215	T5219	nmod	/,fibroblasts
R4598	T5216	T5219	nmod	−,fibroblasts
R4599	T5217	T5219	nmod	mouse,fibroblasts
R4600	T5218	T5219	amod	embryonic,fibroblasts
R4601	T5219	T5207	appos	fibroblasts,HeLa
R4602	T5220	T5219	punct	(,fibroblasts
R4603	T5221	T5219	appos	MEFs,fibroblasts
R4604	T5222	T5219	punct	),fibroblasts
R4605	T5223	T5219	punct	(,fibroblasts
R4606	T5224	T5219	appos	52,fibroblasts
R4607	T5225	T5219	punct	),fibroblasts
R4608	T5226	T5219	cc	and,fibroblasts
R4609	T5227	T5228	amod	293T,cells
R4610	T5228	T5219	conj	cells,fibroblasts
R4611	T5229	T5230	auxpass	were,cultured
R4612	T5230	T5253	ccomp	cultured,cultured
R4613	T5231	T5230	prep	in,cultured
R4614	T5232	T5237	poss	Dulbecco,medium
R4615	T5233	T5232	case	's,Dulbecco
R4616	T5234	T5237	amod	modified,medium
R4617	T5235	T5237	poss	Eagle,medium
R4618	T5236	T5235	case	's,Eagle
R4619	T5237	T5231	pobj	medium,in
R4620	T5238	T5253	punct	;,cultured
R4621	T5239	T5253	nsubjpass	Jurkat,cultured
R4622	T5240	T5239	punct	",",Jurkat
R4623	T5241	T5242	compound	U937,cells
R4624	T5242	T5239	appos	cells,Jurkat
R4625	T5243	T5242	cc	and,cells
R4626	T5244	T5246	amod	human,blood
R4627	T5245	T5246	compound	peripheral,blood
R4628	T5246	T5248	compound	blood,cells
R4629	T5247	T5248	compound	mononuclear,cells
R4630	T5248	T5242	conj	cells,cells
R4631	T5249	T5250	punct	(,PBMCs
R4632	T5250	T5248	appos	PBMCs,cells
R4633	T5251	T5248	punct	),cells
R4634	T5252	T5253	auxpass	were,cultured
R4635	T5253	T5253	ROOT	cultured,cultured
R4636	T5254	T5253	prep	in,cultured
R4637	T5255	T5254	pobj	RPMI,in
R4638	T5256	T5255	nummod	1640,RPMI
R4639	T5257	T5253	punct	.,cultured
R4640	T5258	T5260	nsubjpass	PBMCs,isolated
R4641	T5259	T5260	auxpass	were,isolated
R4642	T5260	T5260	ROOT	isolated,isolated
R4643	T5261	T5263	mark	as,described
R4644	T5262	T5263	advmod	previously,described
R4645	T5263	T5260	advcl	described,isolated
R4646	T5264	T5263	punct	(,described
R4647	T5265	T5263	npadvmod	53,described
R4648	T5266	T5263	punct	),described
R4649	T5267	T5260	punct	.,isolated
R4650	T5268	T5270	nsubjpass	Media,supplemented
R4651	T5269	T5270	auxpass	were,supplemented
R4652	T5270	T5270	ROOT	supplemented,supplemented
R4653	T5271	T5270	prep	with,supplemented
R4654	T5272	T5273	nummod	10,%
R4655	T5273	T5277	nmod	%,serum
R4656	T5274	T5277	amod	heat-inactivated,serum
R4657	T5275	T5276	amod	fetal,calf
R4658	T5276	T5277	compound	calf,serum
R4659	T5277	T5271	pobj	serum,with
R4660	T5278	T5277	cc	and,serum
R4661	T5279	T5280	nummod	2,mM
R4662	T5280	T5281	compound	mM,l-glutamine
R4663	T5281	T5277	conj	l-glutamine,serum
R4664	T5282	T5285	punct	(,AG
R4665	T5283	T5285	compound	Lonza,AG
R4666	T5284	T5285	compound	Cologne,AG
R4667	T5285	T5277	appos	AG,serum
R4668	T5286	T5285	punct	",",AG
R4669	T5287	T5285	npadvmod	Germany,AG
R4670	T5288	T5285	punct	),AG
R4671	T5289	T5270	punct	.,supplemented
R4672	T5290	T5304	nsubjpass	HeLa,transfected
R4673	T5291	T5290	punct	",",HeLa
R4674	T5292	T5300	nummod	p50,MEFs
R4675	T5293	T5299	nmod	−,−
R4676	T5294	T5299	nmod	/,−
R4677	T5295	T5299	nmod	−,−
R4678	T5296	T5299	nmod	p65,−
R4679	T5297	T5299	nmod	−,−
R4680	T5298	T5299	nmod	/,−
R4681	T5299	T5300	nmod	−,MEFs
R4682	T5300	T5290	conj	MEFs,HeLa
R4683	T5301	T5300	cc	and,MEFs
R4684	T5302	T5300	conj	293T,MEFs
R4685	T5303	T5304	auxpass	were,transfected
R4686	T5304	T5332	ccomp	transfected,equalized
R4687	T5305	T5304	prep	with,transfected
R4688	T5306	T5305	pobj	DNA,with
R4689	T5307	T5304	prep	by,transfected
R4690	T5308	T5307	pcomp	using,by
R4691	T5309	T5310	compound	FuGENE,HD
R4692	T5310	T5308	dobj	HD,using
R4693	T5311	T5314	punct	(,GmbH
R4694	T5312	T5314	compound	Roche,GmbH
R4695	T5313	T5314	compound	Diagnostic,GmbH
R4696	T5314	T5308	dobj	GmbH,using
R4697	T5315	T5314	punct	",",GmbH
R4698	T5316	T5314	conj	Mannheim,GmbH
R4699	T5317	T5316	punct	",",Mannheim
R4700	T5318	T5314	npadvmod	Germany,GmbH
R4701	T5319	T5314	punct	),GmbH
R4702	T5320	T5304	punct	",",transfected
R4703	T5321	T5304	prep	according,transfected
R4704	T5322	T5321	prep	to,according
R4705	T5323	T5324	det	the,manufacturer
R4706	T5324	T5326	poss	manufacturer,protocol
R4707	T5325	T5324	case	's,manufacturer
R4708	T5326	T5322	pobj	protocol,to
R4709	T5327	T5332	punct	;,equalized
R4710	T5328	T5330	amod	total,amounts
R4711	T5329	T5330	compound	DNA,amounts
R4712	T5330	T5332	nsubjpass	amounts,equalized
R4713	T5331	T5332	auxpass	were,equalized
R4714	T5332	T5332	ROOT	equalized,equalized
R4715	T5333	T5332	agent	by,equalized
R4716	T5334	T5333	pobj	transfection,by
R4717	T5335	T5334	prep	of,transfection
R4718	T5336	T5338	nmod	pRc/CMV,vector
R4719	T5337	T5338	amod	empty,vector
R4720	T5338	T5335	pobj	vector,of
R4721	T5339	T5340	punct	(,Invitrogen
R4722	T5340	T5338	appos	Invitrogen,vector
R4723	T5341	T5340	punct	",",Invitrogen
R4724	T5342	T5340	npadvmod	Carlsbad,Invitrogen
R4725	T5343	T5342	punct	",",Carlsbad
R4726	T5344	T5342	conj	CA,Carlsbad
R4727	T5345	T5344	punct	",",CA
R4728	T5346	T5344	conj	USA,CA
R4729	T5347	T5340	punct	),Invitrogen
R4730	T5348	T5332	punct	.,equalized
R4731	T5349	T5355	prep	For,treated
R4732	T5350	T5349	pobj	pulse-stimulation,For
R4733	T5351	T5355	punct	",",treated
R4734	T5352	T5353	compound	HeLa,cells
R4735	T5353	T5355	nsubjpass	cells,treated
R4736	T5354	T5355	auxpass	were,treated
R4737	T5355	T5397	ccomp	treated,washed
R4738	T5356	T5355	prep	with,treated
R4739	T5357	T5359	nmod	phorbol,13-acetate
R4740	T5358	T5359	amod	12-myristate,13-acetate
R4741	T5359	T5356	pobj	13-acetate,with
R4742	T5360	T5359	punct	(,13-acetate
R4743	T5361	T5359	appos	PMA,13-acetate
R4744	T5362	T5397	punct	;,washed
R4745	T5363	T5397	nsubj	Sigma-Aldrich,washed
R4746	T5364	T5363	punct	",",Sigma-Aldrich
R4747	T5365	T5366	compound	St,Louis
R4748	T5366	T5363	conj	Louis,Sigma-Aldrich
R4749	T5367	T5366	punct	",",Louis
R4750	T5368	T5366	conj	MO,Louis
R4751	T5369	T5368	punct	",",MO
R4752	T5370	T5368	conj	USA,MO
R4753	T5371	T5363	punct	),Sigma-Aldrich
R4754	T5372	T5374	punct	(,ng/ml
R4755	T5373	T5374	nummod	20,ng/ml
R4756	T5374	T5363	appos	ng/ml,Sigma-Aldrich
R4757	T5375	T5363	punct	),Sigma-Aldrich
R4758	T5376	T5363	prep	for,Sigma-Aldrich
R4759	T5377	T5378	nummod	5,min
R4760	T5378	T5376	pobj	min,for
R4761	T5379	T5378	punct	",",min
R4762	T5380	T5378	cc	or,min
R4763	T5381	T5382	compound	tumor,necrosis
R4764	T5382	T5383	compound	necrosis,factor-α
R4765	T5383	T5378	conj	factor-α,min
R4766	T5384	T5385	punct	(,TNF-α
R4767	T5385	T5383	appos	TNF-α,factor-α
R4768	T5386	T5363	punct	;,Sigma-Aldrich
R4769	T5387	T5363	conj	Sigma-Aldrich,Sigma-Aldrich
R4770	T5388	T5363	punct	),Sigma-Aldrich
R4771	T5389	T5391	punct	(,ng/ml
R4772	T5390	T5391	nummod	20,ng/ml
R4773	T5391	T5363	appos	ng/ml,Sigma-Aldrich
R4774	T5392	T5363	punct	),Sigma-Aldrich
R4775	T5393	T5363	prep	for,Sigma-Aldrich
R4776	T5394	T5395	nummod	30,min
R4777	T5395	T5393	pobj	min,for
R4778	T5396	T5397	punct	",",washed
R4779	T5397	T5397	ROOT	washed,washed
R4780	T5398	T5397	advmod	twice,washed
R4781	T5399	T5397	prep	in,washed
R4782	T5400	T5402	amod	complete,medium
R4783	T5401	T5402	compound	culture,medium
R4784	T5402	T5399	pobj	medium,in
R4785	T5403	T5397	cc	and,washed
R4786	T5404	T5405	advmod	then,returned
R4787	T5405	T5397	conj	returned,washed
R4788	T5406	T5405	prep	to,returned
R4789	T5407	T5406	pobj	culture,to
R4790	T5408	T5397	punct	.,washed
R4791	T5409	T5415	prep	For,co-transfected
R4792	T5410	T5411	compound	luciferase,assays
R4793	T5411	T5409	pobj	assays,For
R4794	T5412	T5415	punct	",",co-transfected
R4795	T5413	T5414	nsubj	pSV-β-Gal,was
R4796	T5414	T5415	auxpass	was,co-transfected
R4797	T5415	T5415	ROOT	co-transfected,co-transfected
R4798	T5416	T5415	prep	with,co-transfected
R4799	T5417	T5416	pobj	pκBluc,with
R4800	T5418	T5419	aux	to,monitor
R4801	T5419	T5415	advcl	monitor,co-transfected
R4802	T5420	T5422	det	the,efficiency
R4803	T5421	T5422	compound	transfection,efficiency
R4804	T5422	T5419	dobj	efficiency,monitor
R4805	T5423	T5415	punct	.,co-transfected
R4806	T5424	T5425	amod	Forty-eight-hour,post-transfection
R4807	T5425	T5429	intj	post-transfection,lysed
R4808	T5426	T5429	punct	",",lysed
R4809	T5427	T5429	nsubjpass	cells,lysed
R4810	T5428	T5429	auxpass	were,lysed
R4811	T5429	T5429	ROOT	lysed,lysed
R4812	T5430	T5429	prep	in,lysed
R4813	T5431	T5432	compound	lysis,buffer
R4814	T5432	T5430	pobj	buffer,in
R4815	T5433	T5432	prep	of,buffer
R4816	T5434	T5435	compound	Dual,Light
R4817	T5435	T5437	compound	Light,System
R4818	T5436	T5437	compound	Luciferase,System
R4819	T5437	T5433	pobj	System,of
R4820	T5438	T5437	punct	(,System
R4821	T5439	T5437	appos	Tropix,System
R4822	T5440	T5439	punct	",",Tropix
R4823	T5441	T5439	conj	Bedford,Tropix
R4824	T5442	T5441	punct	",",Bedford
R4825	T5443	T5441	conj	MA,Bedford
R4826	T5444	T5443	punct	",",MA
R4827	T5445	T5443	conj	USA,MA
R4828	T5446	T5443	punct	),MA
R4829	T5447	T5437	cc	and,System
R4830	T5448	T5449	det	the,luciferase
R4831	T5449	T5452	nmod	luciferase,activities
R4832	T5450	T5449	cc	and,luciferase
R4833	T5451	T5449	conj	β-galactosidase,luciferase
R4834	T5452	T5454	nsubjpass	activities,evaluated
R4835	T5453	T5454	auxpass	were,evaluated
R4836	T5454	T5429	conj	evaluated,lysed
R4837	T5455	T5454	prep	by,evaluated
R4838	T5456	T5455	pcomp	using,by
R4839	T5457	T5458	compound	Dual,Light
R4840	T5458	T5460	compound	Light,System
R4841	T5459	T5460	compound	Luciferase,System
R4842	T5460	T5456	dobj	System,using
R4843	T5461	T5460	punct	(,System
R4844	T5462	T5460	appos	Tropix,System
R4845	T5463	T5460	punct	),System
R4846	T5464	T5456	prep	in,using
R4847	T5465	T5466	det	a,bioluminometer
R4848	T5466	T5464	pobj	bioluminometer,in
R4849	T5467	T5469	punct	(,Biosystem
R4850	T5468	T5469	compound	Turner,Biosystem
R4851	T5469	T5454	npadvmod	Biosystem,evaluated
R4852	T5470	T5469	punct	",",Biosystem
R4853	T5471	T5469	npadvmod	Sunnyvale,Biosystem
R4854	T5472	T5471	punct	",",Sunnyvale
R4855	T5473	T5471	conj	CA,Sunnyvale
R4856	T5474	T5473	punct	",",CA
R4857	T5475	T5473	conj	USA,CA
R4858	T5476	T5473	punct	),CA
R4859	T5477	T5454	punct	.,evaluated
R4860	T5478	T5479	det	The,ratio
R4861	T5479	T5488	nsubjpass	ratio,expressed
R4862	T5480	T5479	prep	of,ratio
R4863	T5481	T5483	amod	firefly,activity
R4864	T5482	T5483	amod	luciferase,activity
R4865	T5483	T5480	pobj	activity,of
R4866	T5484	T5483	prep	to,activity
R4867	T5485	T5486	amod	β-galactosidase,activity
R4868	T5486	T5484	pobj	activity,to
R4869	T5487	T5488	auxpass	was,expressed
R4870	T5488	T5488	ROOT	expressed,expressed
R4871	T5489	T5488	prep	as,expressed
R4872	T5490	T5492	amod	relative,units
R4873	T5491	T5492	amod	light,units
R4874	T5492	T5489	pobj	units,as
R4875	T5493	T5488	punct	.,expressed
R5028	T5824	T5825	compound	RNA,interference
R5029	T5825	T5826	compound	interference,Jurkat
R5030	T5826	T5829	nmod	Jurkat,cells
R5031	T5827	T5826	cc	or,Jurkat
R5032	T5828	T5826	conj	U937,Jurkat
R5033	T5829	T5831	nsubjpass	cells,transfected
R5034	T5830	T5831	auxpass	were,transfected
R5035	T5831	T5831	ROOT	transfected,transfected
R5036	T5832	T5831	agent	by,transfected
R5037	T5833	T5832	pobj	electroporation,by
R5038	T5834	T5831	advcl	using,transfected
R5039	T5835	T5837	det	a,apparatus
R5040	T5836	T5837	compound	Bio-Rad,apparatus
R5041	T5837	T5834	dobj	apparatus,using
R5042	T5838	T5837	punct	(,apparatus
R5043	T5839	T5840	compound	Bio-Rad,Laboratories
R5044	T5840	T5837	appos	Laboratories,apparatus
R5045	T5841	T5840	punct	",",Laboratories
R5046	T5842	T5840	conj	Hercules,Laboratories
R5047	T5843	T5842	punct	",",Hercules
R5048	T5844	T5842	conj	CA,Hercules
R5049	T5845	T5844	punct	",",CA
R5050	T5846	T5844	conj	USA,CA
R5051	T5847	T5837	punct	),apparatus
R5052	T5848	T5831	punct	.,transfected
R5053	T5849	T5859	advmod	Briefly,suspended
R5054	T5850	T5859	punct	",",suspended
R5055	T5851	T5859	nsubjpass	aliquots,suspended
R5056	T5852	T5851	punct	(,aliquots
R5057	T5853	T5856	nummod	5,cells
R5058	T5854	T5856	nummod	×,cells
R5059	T5855	T5856	nummod	106,cells
R5060	T5856	T5851	appos	cells,aliquots
R5061	T5857	T5851	punct	),aliquots
R5062	T5858	T5859	auxpass	were,suspended
R5063	T5859	T5859	ROOT	suspended,suspended
R5064	T5860	T5859	prep	in,suspended
R5065	T5861	T5862	nummod	0.3,ml
R5066	T5862	T5860	pobj	ml,in
R5067	T5863	T5862	prep	of,ml
R5068	T5864	T5865	compound	RPMI,1640
R5069	T5865	T5863	pobj	1640,of
R5070	T5866	T5859	conj	supplemented,suspended
R5071	T5867	T5866	prep	with,supplemented
R5072	T5868	T5869	nummod	20,%
R5073	T5869	T5872	compound	%,serum
R5074	T5870	T5871	amod	fetal,calf
R5075	T5871	T5872	compound	calf,serum
R5076	T5872	T5867	pobj	serum,with
R5077	T5873	T5872	cc	and,serum
R5078	T5874	T5859	conj	subjected,suspended
R5079	T5875	T5874	prep	to,subjected
R5080	T5876	T5879	det	a,pulse
R5081	T5877	T5879	amod	double,pulse
R5082	T5878	T5879	amod	electrical,pulse
R5083	T5879	T5875	pobj	pulse,to
R5084	T5880	T5882	punct	(,V
R5085	T5881	T5882	nummod	0.22,V
R5086	T5882	T5879	appos	V,pulse
R5087	T5883	T5882	punct	",",V
R5088	T5884	T5885	nummod	960,µF
R5089	T5885	T5882	appos	µF,V
R5090	T5886	T5885	punct	),µF
R5091	T5887	T5882	prep	in,V
R5092	T5888	T5889	det	the,presence
R5093	T5889	T5887	pobj	presence,in
R5094	T5890	T5889	prep	of,presence
R5095	T5891	T5892	compound	annealed,siRNA
R5096	T5892	T5890	pobj	siRNA,of
R5097	T5893	T5892	punct	(,siRNA
R5098	T5894	T5895	nummod	200,pmol
R5099	T5895	T5892	appos	pmol,siRNA
R5100	T5896	T5892	punct	),siRNA
R5101	T5897	T5882	punct	;,V
R5102	T5898	T5899	amod	electroporated,cells
R5103	T5899	T5901	nsubjpass	cells,washed
R5104	T5900	T5901	auxpass	were,washed
R5105	T5901	T5859	conj	washed,suspended
R5106	T5902	T5901	cc	and,washed
R5107	T5903	T5901	conj	cultured,washed
R5108	T5904	T5903	prep	in,cultured
R5109	T5905	T5906	amod	complete,medium
R5110	T5906	T5904	pobj	medium,in
R5111	T5907	T5901	punct	.,washed
R5112	T5908	T5909	compound	RNA,interference
R5113	T5909	T5911	nsubjpass	interference,performed
R5114	T5910	T5911	auxpass	was,performed
R5115	T5911	T5911	ROOT	performed,performed
R5116	T5912	T5911	prep	with,performed
R5117	T5913	T5912	punct	:,with
R5118	T5914	T5915	compound	siRNA,Tat
R5119	T5915	T5916	compound	Tat,sense
R5120	T5916	T5912	pobj	sense,with
R5121	T5917	T5916	punct	",",sense
R5122	T5918	T5916	conj	CUGCUUGUACCAAUUGCUAUU,sense
R5123	T5919	T5918	cc	and,CUGCUUGUACCAAUUGCUAUU
R5124	T5920	T5921	compound	siRNA,Tat
R5125	T5921	T5918	conj	Tat,CUGCUUGUACCAAUUGCUAUU
R5126	T5922	T5918	conj	antisense,CUGCUUGUACCAAUUGCUAUU
R5127	T5923	T5922	punct	",",antisense
R5128	T5924	T5922	conj	UAGCAAUUGGUACAAGCAGUU,antisense
R5129	T5925	T5928	punct	;,sense
R5130	T5926	T5928	compound	siRNA,sense
R5131	T5927	T5928	compound	control,sense
R5132	T5928	T5911	npadvmod	sense,performed
R5133	T5929	T5928	punct	",",sense
R5135	T5931	T5928	appos	AUUGCUAUU,sense
R5136	T5932	T5931	cc	and,AUUGCUAUU
R5137	T5933	T5931	conj	siRNA,AUUGCUAUU
R5138	T5934	T5935	compound	control,antisense
R5139	T5935	T5928	conj	antisense,sense
R5140	T5936	T5935	punct	",",antisense
R5141	T5937	T5928	appos	UAGCAAUUGUGACAAGCAGUU,sense
R5142	T5938	T5911	punct	.,performed
R5143	T5939	T5940	compound	RNA,interference
R5144	T5940	T5946	nsubjpass	interference,performed
R5145	T5941	T5940	prep	of,interference
R5146	T5942	T5941	pobj	p65,of
R5147	T5943	T5940	cc	and,interference
R5148	T5944	T5940	conj	IκB-α,interference
R5149	T5945	T5946	auxpass	was,performed
R5150	T5946	T5946	ROOT	performed,performed
R5151	T5947	T5946	prep	with,performed
R5152	T5948	T5949	compound	SMART,pool
R5153	T5949	T5950	compound	pool,siRNA
R5154	T5950	T5947	pobj	siRNA,with
R5155	T5951	T5950	nummod	p65,siRNA
R5156	T5952	T5950	cc	and,siRNA
R5157	T5953	T5950	conj	IκB-α,siRNA
R5158	T5954	T5955	punct	(,Dharmacon
R5159	T5955	T5950	conj	Dharmacon,siRNA
R5160	T5956	T5955	punct	",",Dharmacon
R5161	T5957	T5955	npadvmod	Chicago,Dharmacon
R5162	T5958	T5955	punct	",",Dharmacon
R5163	T5959	T5955	conj	IL,Dharmacon
R5164	T5960	T5959	punct	",",IL
R5165	T5961	T5959	conj	USA,IL
R5166	T5962	T5946	punct	),performed
R5167	T5963	T5946	punct	.,performed
R5255	T6139	T6140	compound	Pseudotyped,virions
R5256	T6140	T6153	nsubjpass	virions,transfected
R5257	T6141	T6140	cc	and,virions
R5258	T6142	T6146	amod	single,cells
R5259	T6143	T6146	amod	round,cells
R5260	T6144	T6146	nmod	infection,cells
R5261	T6145	T6146	amod	293T,cells
R5262	T6146	T6140	conj	cells,virions
R5263	T6147	T6148	punct	(,1
R5264	T6148	T6150	nummod	1,107
R5265	T6149	T6150	nummod	×,107
R5266	T6150	T6146	appos	107,cells
R5267	T6151	T6146	punct	),cells
R5268	T6152	T6153	auxpass	were,transfected
R5269	T6153	T6153	ROOT	transfected,transfected
R5270	T6154	T6153	prep	with,transfected
R5271	T6155	T6154	pobj	pNL4-3,with
R5272	T6156	T6153	punct	.,transfected
R5273	T6157	T6153	punct	Luc.R-E,transfected
R5274	T6158	T6157	punct	-,Luc.R-E
R5275	T6159	T6157	cc	or,Luc.R-E
R5276	T6160	T6157	conj	pNL4-3,Luc.R-E
R5277	T6161	T6153	punct	.,transfected
R5278	T6162	T6162	ROOT	FLAG-Tat,FLAG-Tat
R5279	T6163	T6162	punct	.,FLAG-Tat
R5280	T6164	T6162	punct	R-E,FLAG-Tat
R5281	T6165	T6164	punct	-,R-E
R5282	T6166	T6164	punct	(,R-E
R5283	T6167	T6168	nummod	10,µg
R5284	T6168	T6164	appos	µg,R-E
R5285	T6169	T6164	punct	),R-E
R5286	T6170	T6171	advmod	together,with
R5287	T6171	T6185	prep	with,collected
R5288	T6172	T6173	compound	pHXB2,Env
R5289	T6173	T6171	pobj	Env,with
R5290	T6174	T6176	punct	(,µg
R5291	T6175	T6176	nummod	10,µg
R5292	T6176	T6173	appos	µg,Env
R5293	T6177	T6176	punct	),µg
R5294	T6178	T6173	punct	",",Env
R5295	T6179	T6173	cc	and,Env
R5296	T6180	T6183	amod	48-h,supernatant
R5297	T6181	T6182	amod	post-transfection,cell
R5298	T6182	T6183	compound	cell,supernatant
R5299	T6183	T6185	nsubjpass	supernatant,collected
R5300	T6184	T6185	auxpass	was,collected
R5301	T6185	T6185	ROOT	collected,collected
R5302	T6186	T6185	punct	.,collected
R5303	T6187	T6189	amod	Enzyme-linked,assay
R5304	T6188	T6189	compound	immunosorbent,assay
R5305	T6189	T6189	ROOT	assay,assay
R5306	T6190	T6189	punct	(,assay
R5307	T6191	T6189	appos	ELISA,assay
R5308	T6192	T6189	punct	),assay
R5309	T6193	T6189	xcomp	using,assay
R5310	T6194	T6195	amod	anti-p24,antibody
R5311	T6195	T6193	dobj	antibody,using
R5312	T6196	T6198	amod	measured,concentration
R5313	T6197	T6198	compound	virion,concentration
R5314	T6198	T6193	dobj	concentration,using
R5315	T6199	T6189	punct	.,assay
R5316	T6200	T6212	nsubjpass	PBMCs,infected
R5317	T6201	T6200	punct	",",PBMCs
R5318	T6202	T6200	conj	Jurkat,PBMCs
R5319	T6203	T6202	cc	or,Jurkat
R5320	T6204	T6202	conj	U937,Jurkat
R5321	T6205	T6202	conj	cells,Jurkat
R5322	T6206	T6205	punct	(,cells
R5323	T6207	T6209	quantmod	5,107
R5324	T6208	T6209	quantmod	×,107
R5325	T6209	T6205	appos	107,cells
R5326	T6210	T6205	punct	),cells
R5327	T6211	T6212	auxpass	were,infected
R5328	T6212	T6212	ROOT	infected,infected
R5329	T6213	T6212	prep	with,infected
R5330	T6214	T6216	nmod	HXB2,virions
R5331	T6215	T6216	compound	Env-pseudotyped,virions
R5332	T6216	T6213	pobj	virions,with
R5333	T6217	T6219	punct	(,ng
R5334	T6218	T6219	nummod	500,ng
R5335	T6219	T6216	appos	ng,virions
R5336	T6220	T6219	prep	of,ng
R5337	T6221	T6220	pobj	p24,of
R5338	T6222	T6219	punct	),ng
R5339	T6223	T6219	prep	by,ng
R5340	T6224	T6223	pobj	spinoculation,by
R5341	T6225	T6219	punct	",",ng
R5342	T6226	T6228	mark	as,described
R5343	T6227	T6228	advmod	previously,described
R5344	T6228	T6219	advcl	described,ng
R5345	T6229	T6228	punct	(,described
R5346	T6230	T6228	npadvmod	50,described
R5347	T6231	T6219	punct	),ng
R5348	T6232	T6212	punct	.,infected
R5358	T6307	T6308	compound	Cell,extracts
R5359	T6308	T6326	nsubjpass	extracts,performed
R5360	T6309	T6308	punct	",",extracts
R5361	T6310	T6311	amod	western,blotting
R5362	T6311	T6326	nsubjpass	blotting,performed
R5363	T6312	T6311	punct	",",blotting
R5364	T6313	T6314	compound	IKK,activity
R5365	T6314	T6311	conj	activity,blotting
R5366	T6315	T6314	cc	and,activity
R5367	T6316	T6317	compound	NF-κB,DNA
R5368	T6317	T6314	conj	DNA,activity
R5369	T6318	T6319	amod	binding,Total
R5370	T6319	T6314	conj	Total,activity
R5371	T6320	T6319	punct	",",Total
R5372	T6321	T6324	amod	nuclear,extracts
R5373	T6322	T6321	cc	and,nuclear
R5374	T6323	T6321	conj	cytosolic,nuclear
R5375	T6324	T6314	conj	extracts,activity
R5376	T6325	T6326	auxpass	were,performed
R5377	T6326	T6336	ccomp	performed,reported
R5378	T6327	T6329	mark	as,described
R5379	T6328	T6329	advmod	previously,described
R5380	T6329	T6326	advcl	described,performed
R5381	T6330	T6329	punct	(,described
R5382	T6331	T6329	npadvmod	54,described
R5383	T6332	T6329	punct	),described
R5384	T6333	T6336	punct	;,reported
R5385	T6334	T6336	nsubjpass	details,reported
R5386	T6335	T6336	auxpass	are,reported
R5387	T6336	T6336	ROOT	reported,reported
R5388	T6337	T6336	prep	in,reported
R5389	T6338	T6339	compound	Supplementary,Data
R5390	T6339	T6337	pobj	Data,in
R5391	T6340	T6336	punct	.,reported
R5392	T6341	T6343	amod	Western,analysis
R5393	T6342	T6343	amod	blotting,analysis
R5394	T6343	T6345	nsubjpass	analysis,performed
R5395	T6344	T6345	auxpass	was,performed
R5396	T6345	T6345	ROOT	performed,performed
R5397	T6346	T6345	agent	by,performed
R5398	T6347	T6380	advcl	resuspending,resolved
R5399	T6348	T6349	compound	protein,aliquots
R5400	T6349	T6347	dobj	aliquots,resuspending
R5401	T6350	T6347	prep	in,resuspending
R5402	T6351	T6350	pcomp	loading,in
R5403	T6352	T6351	dobj	buffer,loading
R5404	T6353	T6352	punct	(,buffer
R5405	T6354	T6356	nummod	125,Tris
R5406	T6355	T6356	compound	mM,Tris
R5407	T6356	T6352	appos	Tris,buffer
R5408	T6357	T6380	nsubj	HCl,resolved
R5409	T6358	T6357	punct	",",HCl
R5410	T6359	T6357	appos	pH,HCl
R5411	T6360	T6359	nummod	6.8,pH
R5412	T6361	T6359	punct	",",pH
R5413	T6362	T6363	nummod	5,%
R5414	T6363	T6364	npadvmod	%,SDS
R5415	T6364	T6357	conj	SDS,HCl
R5416	T6365	T6364	punct	",",SDS
R5417	T6366	T6367	nummod	1,%
R5418	T6367	T6369	npadvmod	%,blue
R5419	T6368	T6369	compound	bromophenol,blue
R5420	T6369	T6373	amod	blue,β-mercaptoethanol
R5421	T6370	T6373	punct	",",β-mercaptoethanol
R5422	T6371	T6372	nummod	10,%
R5423	T6372	T6373	compound	%,β-mercaptoethanol
R5424	T6373	T6364	appos	β-mercaptoethanol,SDS
R5425	T6374	T6364	punct	",",SDS
R5426	T6375	T6376	nummod	25,%
R5427	T6376	T6377	compound	%,glycerol
R5428	T6377	T6364	appos	glycerol,SDS
R5429	T6378	T6364	punct	),SDS
R5430	T6379	T6364	punct	",",SDS
R5431	T6380	T6346	pcomp	resolved,by
R5432	T6381	T6380	prep	on,resolved
R5433	T6382	T6383	nummod	12,%
R5434	T6383	T6384	compound	%,SDS
R5435	T6384	T6381	pobj	SDS,on
R5436	T6385	T6384	appos	PAGE,SDS
R5437	T6386	T6384	punct	",",SDS
R5438	T6387	T6345	advcl	transferred,performed
R5439	T6388	T6389	aux	to,polyvinylidene
R5440	T6389	T6387	xcomp	polyvinylidene,transferred
R5441	T6390	T6391	compound	difluoride,membrane
R5442	T6391	T6389	dobj	membrane,polyvinylidene
R5443	T6392	T6393	punct	(,Millipore
R5444	T6393	T6391	appos	Millipore,membrane
R5445	T6394	T6393	punct	",",Millipore
R5446	T6395	T6393	conj	Bedford,Millipore
R5447	T6396	T6395	punct	",",Bedford
R5448	T6397	T6395	conj	MA,Bedford
R5449	T6398	T6397	punct	",",MA
R5450	T6399	T6397	conj	USA,MA
R5451	T6400	T6397	punct	),MA
R5452	T6401	T6387	cc	and,transferred
R5453	T6402	T6387	conj	incubated,transferred
R5454	T6403	T6402	prep	with,incubated
R5455	T6404	T6405	amod	primary,antibodies
R5456	T6405	T6403	pobj	antibodies,with
R5457	T6406	T6405	punct	(,antibodies
R5458	T6407	T6405	appos	1:1000,antibodies
R5459	T6408	T6405	punct	),antibodies
R5460	T6409	T6387	conj	followed,transferred
R5461	T6410	T6409	agent	by,followed
R5462	T6411	T6410	pobj	incubation,by
R5463	T6412	T6409	prep	with,followed
R5464	T6413	T6414	amod	horseradish-peroxidase-linked,mouse
R5465	T6414	T6417	nmod	mouse,IgG
R5466	T6415	T6414	cc	or,mouse
R5467	T6416	T6414	conj	rabbit,mouse
R5468	T6417	T6412	pobj	IgG,with
R5469	T6418	T6417	punct	(,IgG
R5470	T6419	T6417	appos	1:2000,IgG
R5471	T6420	T6417	punct	),IgG
R5472	T6421	T6424	punct	(,Amersham
R5473	T6422	T6424	compound	GE,Amersham
R5474	T6423	T6424	compound	Healthcare,Amersham
R5475	T6424	T6417	appos	Amersham,IgG
R5476	T6425	T6424	punct	",",Amersham
R5477	T6426	T6427	compound	Little,Chalfont
R5478	T6427	T6424	npadvmod	Chalfont,Amersham
R5479	T6428	T6427	punct	",",Chalfont
R5480	T6429	T6427	conj	Buckinghamshire,Chalfont
R5481	T6430	T6429	punct	",",Buckinghamshire
R5482	T6431	T6429	appos	UK,Buckinghamshire
R5483	T6432	T6429	punct	),Buckinghamshire
R5484	T6433	T6424	prep	in,Amersham
R5485	T6434	T6433	pobj	PBS,in
R5486	T6435	T6417	acl	containing,IgG
R5487	T6436	T6437	nummod	5,%
R5488	T6437	T6440	nmod	%,milk
R5489	T6438	T6440	amod	non-fat,milk
R5490	T6439	T6440	amod	dry,milk
R5491	T6440	T6435	dobj	milk,containing
R5492	T6441	T6443	punct	(,Laboratories
R5493	T6442	T6443	compound	Bio-Rad,Laboratories
R5494	T6443	T6440	appos	Laboratories,milk
R5495	T6444	T6440	punct	),milk
R5496	T6445	T6345	punct	.,performed
R5497	T6446	T6448	nsubjpass	Proteins,detected
R5498	T6447	T6448	auxpass	were,detected
R5499	T6448	T6448	ROOT	detected,detected
R5500	T6449	T6448	agent	by,detected
R5501	T6450	T6449	pobj	chemiluminescence,by
R5502	T6451	T6448	advcl	using,detected
R5503	T6452	T6454	det	the,System
R5504	T6453	T6454	compound	ECL,System
R5505	T6454	T6451	dobj	System,using
R5506	T6455	T6454	punct	(,System
R5507	T6456	T6458	compound	GE,Amersham
R5508	T6457	T6458	compound	Healthcare,Amersham
R5509	T6458	T6454	appos	Amersham,System
R5510	T6459	T6454	punct	),System
R5511	T6460	T6448	punct	.,detected
R5512	T6461	T6462	amod	Primary,antibodies
R5513	T6462	T6464	nsubjpass	antibodies,purchased
R5514	T6463	T6464	auxpass	were,purchased
R5515	T6464	T6464	ROOT	purchased,purchased
R5516	T6465	T6464	prep	from,purchased
R5517	T6466	T6465	punct	:,from
R5518	T6467	T6469	compound	Santa,Biotechnology
R5519	T6468	T6469	compound	Cruz,Biotechnology
R5520	T6469	T6465	pobj	Biotechnology,from
R5521	T6470	T6469	punct	",",Biotechnology
R5522	T6471	T6472	compound	Santa,Cruz
R5523	T6472	T6469	conj	Cruz,Biotechnology
R5524	T6473	T6472	punct	",",Cruz
R5525	T6474	T6472	conj	CA,Cruz
R5526	T6475	T6474	punct	",",CA
R5527	T6476	T6474	conj	USA,CA
R5528	T6477	T6479	punct	(,F7
R5529	T6478	T6479	compound	anti-HA,F7
R5530	T6479	T6474	conj	F7,CA
R5531	T6480	T6479	punct	",",F7
R5532	T6481	T6482	compound	anti-IκB-α,C15
R5533	T6482	T6479	conj	C15,F7
R5534	T6483	T6482	punct	",",C15
R5535	T6484	T6485	compound	anti-Histone,H1
R5536	T6485	T6482	conj	H1,C15
R5537	T6486	T6485	punct	",",H1
R5538	T6487	T6485	conj	anti-Hexokinase-II,H1
R5539	T6488	T6479	punct	),F7
R5540	T6489	T6464	punct	;,purchased
R5541	T6490	T6490	ROOT	Sigma-Aldrich,Sigma-Aldrich
R5542	T6491	T6493	punct	(,M2
R5543	T6492	T6493	amod	anti-FLAG,M2
R5544	T6493	T6490	appos	M2,Sigma-Aldrich
R5545	T6494	T6493	punct	",",M2
R5546	T6495	T6493	amod	anti-γ-Tubulin,M2
R5547	T6496	T6490	punct	),Sigma-Aldrich
R5548	T6497	T6490	punct	;,Sigma-Aldrich
R5549	T6498	T6490	conj	Upstate,Sigma-Aldrich
R5550	T6499	T6498	punct	",",Upstate
R5551	T6500	T6501	compound	Lake,Placid
R5552	T6501	T6498	conj	Placid,Upstate
R5553	T6502	T6501	punct	",",Placid
R5554	T6503	T6501	conj	NY,Placid
R5555	T6504	T6503	punct	",",NY
R5556	T6505	T6503	conj	USA,NY
R5557	T6506	T6507	punct	(,anti-p65
R5558	T6507	T6490	appos	anti-p65,Sigma-Aldrich
R5559	T6508	T6507	punct	),anti-p65
R5560	T6509	T6490	punct	.,Sigma-Aldrich
R5561	T6510	T6515	nsubjpass	Densitometry,analysed
R5562	T6511	T6510	prep	of,Densitometry
R5563	T6512	T6513	amod	single,bands
R5564	T6513	T6511	pobj	bands,of
R5565	T6514	T6515	auxpass	was,analysed
R5566	T6515	T6515	ROOT	analysed,analysed
R5567	T6516	T6515	agent	by,analysed
R5568	T6517	T6519	compound	ImageJ,package
R5569	T6518	T6519	compound	software,package
R5570	T6519	T6516	pobj	package,by
R5571	T6520	T6521	punct	(,NIH
R5572	T6521	T6519	amod	NIH,package
R5573	T6522	T6521	punct	",",NIH
R5574	T6523	T6521	appos	USA,NIH
R5575	T6524	T6521	punct	),NIH
R5576	T6525	T6515	punct	.,analysed
R5577	T6526	T6527	compound	IKK,activity
R5578	T6527	T6529	nsubjpass	activity,evaluated
R5579	T6528	T6529	auxpass	was,evaluated
R5580	T6529	T6529	ROOT	evaluated,evaluated
R5581	T6530	T6529	prep	in,evaluated
R5582	T6531	T6532	amod	cytosolic,extracts
R5583	T6532	T6530	pobj	extracts,in
R5584	T6533	T6529	advcl	using,evaluated
R5585	T6534	T6539	det	the,kit
R5586	T6535	T6539	compound	HTScan,kit
R5587	T6536	T6539	compound	IKK,kit
R5588	T6537	T6538	compound	kinase,assay
R5589	T6538	T6539	compound	assay,kit
R5590	T6539	T6533	dobj	kit,using
R5591	T6540	T6539	punct	(,kit
R5592	T6541	T6543	compound	Cell,Technology
R5593	T6542	T6543	compound	Signaling,Technology
R5594	T6543	T6539	appos	Technology,kit
R5595	T6544	T6543	punct	",",Technology
R5597	T6546	T6545	punct	",",Danvers
R5598	T6547	T6545	conj	MA,Danvers
R5599	T6548	T6547	punct	",",MA
R5600	T6549	T6547	conj	USA,MA
R5601	T6550	T6543	punct	),Technology
R5602	T6551	T6529	punct	.,evaluated
R5603	T6552	T6565	nsubjpass	Binding,measured
R5604	T6553	T6552	prep	of,Binding
R5605	T6554	T6553	pobj	p65,of
R5606	T6555	T6552	punct	",",Binding
R5607	T6556	T6552	appos	p50,Binding
R5608	T6557	T6556	cc	and,p50
R5609	T6558	T6556	conj	FLAG-Tat,p50
R5610	T6559	T6552	prep	to,Binding
R5611	T6560	T6563	det	the,oligonucleotide
R5612	T6561	T6563	amod	double-stranded,oligonucleotide
R5613	T6562	T6563	compound	NF-κB,oligonucleotide
R5614	T6563	T6559	pobj	oligonucleotide,to
R5615	T6564	T6565	auxpass	was,measured
R5616	T6565	T6565	ROOT	measured,measured
R5617	T6566	T6565	advcl	using,measured
R5618	T6567	T6568	compound	NF-κB,Combo
R5619	T6568	T6572	compound	Combo,kit
R5620	T6569	T6572	compound	Transcription,kit
R5621	T6570	T6572	compound	Factor,kit
R5622	T6571	T6572	compound	Assay,kit
R5623	T6572	T6566	dobj	kit,using
R5624	T6573	T6576	punct	(,Company
R5625	T6574	T6576	compound	Cayman,Company
R5626	T6575	T6576	compound	Chemical,Company
R5627	T6576	T6566	parataxis	Company,using
R5628	T6577	T6576	punct	",",Company
R5629	T6578	T6579	compound	Ann,Arbor
R5630	T6579	T6576	conj	Arbor,Company
R5631	T6580	T6579	punct	",",Arbor
R5632	T6581	T6579	conj	MI,Arbor
R5633	T6582	T6581	punct	",",MI
R5634	T6583	T6581	conj	USA,MI
R5635	T6584	T6576	punct	),Company
R5636	T6585	T6565	punct	.,measured
R5637	T6586	T6589	compound	Electrophoretic,Assay
R5638	T6587	T6588	compound	Mobility,Shift
R5639	T6588	T6589	compound	Shift,Assay
R5640	T6589	T6594	nsubjpass	Assay,performed
R5641	T6590	T6591	punct	(,EMSA
R5642	T6591	T6589	appos	EMSA,Assay
R5643	T6592	T6589	punct	),Assay
R5644	T6593	T6594	auxpass	was,performed
R5645	T6594	T6604	ccomp	performed,described
R5646	T6595	T6597	mark	as,described
R5647	T6596	T6597	advmod	previously,described
R5648	T6597	T6594	advcl	described,performed
R5649	T6598	T6597	punct	(,described
R5650	T6599	T6597	npadvmod	55,described
R5651	T6600	T6597	punct	),described
R5652	T6601	T6604	punct	;,described
R5653	T6602	T6604	nsubjpass	details,described
R5654	T6603	T6604	auxpass	are,described
R5655	T6604	T6604	ROOT	described,described
R5656	T6605	T6604	prep	in,described
R5657	T6606	T6607	compound	Supplementary,Data
R5658	T6607	T6605	pobj	Data,in
R5659	T6608	T6604	punct	.,described
R5748	T6765	T6774	prep	In,expressed
R5749	T6766	T6767	amod	vitro,translation
R5750	T6767	T6765	pobj	translation,In
R5751	T6768	T6774	nsubjpass	HA-IκB-α,expressed
R5752	T6769	T6768	punct	",",HA-IκB-α
R5753	T6770	T6768	conj	p65,HA-IκB-α
R5754	T6771	T6770	cc	and,p65
R5755	T6772	T6770	conj	Tat,p65
R5756	T6773	T6774	auxpass	were,expressed
R5757	T6774	T6774	ROOT	expressed,expressed
R5758	T6775	T6774	prep	under,expressed
R5759	T6776	T6778	det	the,promoter
R5760	T6777	T6778	nummod	T7,promoter
R5761	T6778	T6775	pobj	promoter,under
R5762	T6779	T6775	cc	and,under
R5763	T6780	T6775	conj	in,under
R5764	T6781	T6782	amod	vitro,translated
R5765	T6782	T6774	conj	translated,expressed
R5766	T6783	T6782	advcl	using,translated
R5767	T6784	T6789	det	the,system
R5768	T6785	T6789	nmod	TnT,system
R5769	T6786	T6789	amod	quick,system
R5770	T6787	T6789	amod	coupled,system
R5771	T6788	T6789	compound	transcription/translation,system
R5772	T6789	T6783	dobj	system,using
R5773	T6790	T6789	punct	(,system
R5774	T6791	T6789	appos	Promega,system
R5775	T6792	T6789	punct	),system
R5776	T6793	T6783	punct	",",using
R5777	T6794	T6796	mark	as,reported
R5778	T6795	T6796	advmod	previously,reported
R5779	T6796	T6783	advcl	reported,using
R5780	T6797	T6783	punct	(,using
R5781	T6798	T6783	npadvmod	50,using
R5782	T6799	T6783	punct	),using
R5783	T6800	T6774	punct	.,expressed
R5784	T6801	T6803	nsubjpass	Details,described
R5785	T6802	T6803	auxpass	are,described
R5786	T6803	T6803	ROOT	described,described
R5787	T6804	T6803	prep	in,described
R5788	T6805	T6806	compound	Supplementary,Data
R5789	T6806	T6804	pobj	Data,in
R5790	T6807	T6803	punct	.,described
R5860	T6916	T6917	compound	Immunoprecipitation,assay
R5861	T6917	T6934	nsubj	assay,strain
R5862	T6918	T6917	cc	and,assay
R5863	T6919	T6917	conj	GST-pull,assay
R5864	T6920	T6919	prt	down,GST-pull
R5865	T6921	T6919	conj	Immunoprecipitation,GST-pull
R5866	T6922	T6921	punct	",",Immunoprecipitation
R5867	T6923	T6921	conj	GST-pull,Immunoprecipitation
R5868	T6924	T6917	advmod	down,assay
R5869	T6925	T6917	punct	",",assay
R5870	T6926	T6917	cc	and,assay
R5871	T6927	T6917	conj	production,assay
R5872	T6928	T6917	prep	of,assay
R5873	T6929	T6930	compound	GST,proteins
R5874	T6930	T6928	pobj	proteins,of
R5875	T6931	T6917	prep	in,assay
R5876	T6932	T6933	compound	Escherichia,coli
R5877	T6933	T6931	pobj	coli,in
R5878	T6934	T6934	ROOT	strain,strain
R5879	T6935	T6937	nsubjpass	BL21,performed
R5880	T6936	T6937	auxpass	were,performed
R5881	T6937	T6934	ccomp	performed,strain
R5882	T6938	T6940	mark	as,reported
R5883	T6939	T6940	advmod	previously,reported
R5884	T6940	T6937	advcl	reported,performed
R5885	T6941	T6940	punct	(,reported
R5886	T6942	T6940	npadvmod	50,reported
R5887	T6943	T6940	punct	),reported
R5888	T6944	T6934	punct	.,strain
R5889	T6945	T6947	nsubjpass	Details,described
R5890	T6946	T6947	auxpass	are,described
R5891	T6947	T6947	ROOT	described,described
R5892	T6948	T6947	prep	in,described
R5893	T6949	T6950	compound	Supplementary,Data
R5894	T6950	T6948	pobj	Data,in
R5895	T6951	T6947	punct	.,described
R6071	T7201	T7204	amod	Real-time,RNA
R6072	T7202	T7204	compound	PCR,RNA
R6073	T7203	T7204	compound	Total,RNA
R6074	T7204	T7206	nsubjpass	RNA,extracted
R6075	T7205	T7206	auxpass	was,extracted
R6076	T7206	T7226	ccomp	extracted,transcribed
R6077	T7207	T7206	prep	from,extracted
R6078	T7208	T7207	pobj	cells,from
R6079	T7209	T7206	prep	by,extracted
R6080	T7210	T7209	pcomp	using,by
R6081	T7211	T7213	det	the,reagent
R6082	T7212	T7213	compound	TRIzol,reagent
R6083	T7213	T7210	dobj	reagent,using
R6084	T7214	T7213	punct	(,reagent
R6085	T7215	T7213	appos	Invitrogen,reagent
R6086	T7216	T7213	punct	),reagent
R6087	T7217	T7226	punct	;,transcribed
R6088	T7218	T7219	compound	RNA,aliquots
R6089	T7219	T7226	nsubjpass	aliquots,transcribed
R6090	T7220	T7219	punct	(,aliquots
R6091	T7221	T7222	nummod	200,ng
R6092	T7222	T7219	appos	ng,aliquots
R6093	T7223	T7219	punct	),aliquots
R6094	T7224	T7226	auxpass	were,transcribed
R6095	T7225	T7226	advmod	reverse,transcribed
R6096	T7226	T7226	ROOT	transcribed,transcribed
R6097	T7227	T7226	advcl	using,transcribed
R6098	T7228	T7229	compound	Random,Examers
R6099	T7229	T7227	dobj	Examers,using
R6100	T7230	T7229	punct	(,Examers
R6101	T7231	T7229	appos	Roche,Examers
R6102	T7232	T7229	punct	),Examers
R6103	T7233	T7229	cc	and,Examers
R6104	T7234	T7235	compound	Superscript,III
R6105	T7235	T7229	conj	III,Examers
R6106	T7236	T7227	xcomp	Reverse,using
R6107	T7237	T7236	npadvmod	Transcriptase,Reverse
R6108	T7238	T7237	punct	(,Transcriptase
R6109	T7239	T7237	appos	Invitrogen,Transcriptase
R6110	T7240	T7237	punct	),Transcriptase
R6111	T7241	T7236	punct	",",Reverse
R6112	T7242	T7226	prep	according,transcribed
R6113	T7243	T7242	prep	to,according
R6114	T7244	T7245	det	the,manufacturer
R6115	T7245	T7247	poss	manufacturer,protocol
R6116	T7246	T7245	case	's,manufacturer
R6117	T7247	T7243	pobj	protocol,to
R6118	T7248	T7226	punct	.,transcribed
R6119	T7249	T7250	compound	Real-time,PCR
R6120	T7250	T7252	nsubjpass	PCR,performed
R6121	T7251	T7252	auxpass	was,performed
R6122	T7252	T7252	ROOT	performed,performed
R6123	T7253	T7252	prep	with,performed
R6124	T7254	T7258	det	the,mix
R6125	T7255	T7257	compound	iQ,Super
R6126	T7256	T7257	compound	Green,Super
R6127	T7257	T7258	compound	Super,mix
R6128	T7258	T7253	pobj	mix,with
R6129	T7259	T7261	punct	(,Laboratories
R6161	T7291	T7292	compound	°,C
R6162	T7292	T7284	appos	C,C
R6163	T7293	T7292	punct	",",C
R6164	T7294	T7295	nsubj	10,s
R6165	T7295	T7292	appos	s,C
R6166	T7296	T7292	punct	;,C
R6167	T7297	T7298	nummod	60,°
R6168	T7298	T7299	compound	°,C
R6169	T7299	T7292	conj	C,C
R6170	T7300	T7299	punct	",",C
R6171	T7301	T7302	nummod	30,s
R6172	T7302	T7299	appos	s,C
R6173	T7303	T7299	punct	),C
R6174	T7304	T7305	advmod	×,40
R6175	T7305	T7292	appos	40,C
R6176	T7306	T7252	punct	.,performed
R6177	T7307	T7313	nsubjpass	Primers,listed
R6178	T7308	T7307	prep	for,Primers
R6179	T7309	T7308	pobj	Tat,for
R6180	T7310	T7309	cc	and,Tat
R6181	T7311	T7309	conj	MIP-1α,Tat
R6182	T7312	T7313	auxpass	are,listed
R6183	T7313	T7313	ROOT	listed,listed
R6184	T7314	T7313	prep	in,listed
R6185	T7315	T7316	compound	Supplementary,Data
R6186	T7316	T7314	pobj	Data,in
R6187	T7317	T7313	punct	.,listed
R6188	T7318	T7319	amod	Real-time,PCR
R6189	T7319	T7333	nsubjpass	PCR,performed
R6190	T7320	T7319	prep	of,PCR
R6191	T7321	T7320	pobj	CSF3,of
R6192	T7322	T7321	punct	",",CSF3
R6193	T7323	T7321	conj	LTA,CSF3
R6194	T7324	T7323	punct	",",LTA
R6195	T7325	T7323	conj	NFKBIA,LTA
R6196	T7326	T7325	punct	",",NFKBIA
R6197	T7327	T7325	conj	TLR2,NFKBIA
R6198	T7328	T7327	punct	",",TLR2
R6199	T7329	T7327	conj	GAPDH,TLR2
R6200	T7330	T7329	cc	and,GAPDH
R6201	T7331	T7329	conj	ACTB,GAPDH
R6202	T7332	T7333	auxpass	was,performed
R6203	T7333	T7333	ROOT	performed,performed
R6204	T7334	T7333	advcl	using,performed
R6205	T7335	T7337	det	the,profiler
R6206	T7336	T7337	compound	RT2,profiler
R6207	T7337	T7342	nmod	profiler,pathway
R6208	T7338	T7340	compound	PCR,NF-κB
R6209	T7339	T7340	compound	Array-Human,NF-κB
R6210	T7340	T7337	appos	NF-κB,profiler
R6211	T7341	T7342	compound	signaling,pathway
R6212	T7342	T7334	dobj	pathway,using
R6213	T7343	T7342	punct	(,pathway
R6214	T7344	T7345	compound	QIAGEN,Sciences
R6215	T7345	T7342	appos	Sciences,pathway
R6216	T7346	T7345	punct	",",Sciences
R6217	T7347	T7345	conj	MD,Sciences
R6218	T7348	T7345	punct	",",Sciences
R6219	T7349	T7345	appos	USA,Sciences
R6220	T7350	T7342	punct	),pathway
R6221	T7351	T7333	punct	.,performed
R6222	T7352	T7354	nsubjpass	Reactions,carried
R6223	T7353	T7354	auxpass	were,carried
R6224	T7354	T7354	ROOT	carried,carried
R6225	T7355	T7354	prt	out,carried
R6226	T7356	T7354	prep	in,carried
R6227	T7357	T7356	pobj	triplicate,in
R6228	T7358	T7354	punct	",",carried
R6229	T7359	T7354	cc	and,carried
R6230	T7360	T7361	compound	gene,expression
R6231	T7361	T7362	compound	expression,levels
R6232	T7362	T7364	nsubjpass	levels,calculated
R6233	T7363	T7364	auxpass	were,calculated
R6234	T7364	T7354	conj	calculated,carried
R6235	T7365	T7364	oprd	relative,calculated
R6236	T7366	T7365	prep	to,relative
R6237	T7367	T7369	compound	GAPDH,levels
R6238	T7368	T7369	compound	mRNA,levels
R6239	T7369	T7366	pobj	levels,to
R6240	T7370	T7365	prep	as,relative
R6241	T7371	T7372	amod	endogenous,control
R6242	T7372	T7370	pobj	control,as
R6243	T7373	T7364	punct	.,calculated
R6244	T7374	T7375	amod	Relative,expression
R6245	T7375	T7377	nsubjpass	expression,calculated
R6246	T7376	T7377	auxpass	was,calculated
R6247	T7377	T7377	ROOT	calculated,calculated
R6248	T7378	T7377	prep	as,calculated
R6249	T7379	T7382	nummod	2,gene
R6250	T7380	T7382	punct	(,gene
R6251	T7381	T7382	compound	Ct,gene
R6252	T7382	T7378	pobj	gene,as
R6253	T7383	T7382	prep	under,gene
R6254	T7384	T7383	pobj	investigation,under
R6255	T7385	T7387	compound	−,GAPDH
R6256	T7386	T7387	compound	Ct,GAPDH
R6257	T7387	T7382	appos	GAPDH,gene
R6258	T7388	T7382	punct	),gene
R6259	T7389	T7377	punct	.,calculated
R6267	T7546	T7549	amod	Chromatin,Cells
R6268	T7547	T7549	compound	immunoprecipitation,Cells
R6269	T7548	T7549	compound	assay,Cells
R6270	T7549	T7551	nsubjpass	Cells,fixed
R6271	T7550	T7551	auxpass	were,fixed
R6272	T7551	T7551	ROOT	fixed,fixed
R6273	T7552	T7551	prep	by,fixed
R6274	T7553	T7552	pcomp	adding,by
R6275	T7554	T7553	dobj	formaldehyde,adding
R6276	T7555	T7556	punct	(,Sigma-Aldrich
R6277	T7556	T7554	appos	Sigma-Aldrich,formaldehyde
R6278	T7557	T7556	punct	),Sigma-Aldrich
R6279	T7558	T7553	prep	at,adding
R6280	T7559	T7561	det	the,concentration
R6281	T7560	T7561	amod	final,concentration
R6282	T7561	T7558	pobj	concentration,at
R6283	T7562	T7561	prep	of,concentration
R6284	T7563	T7564	nummod	1,%
R6285	T7564	T7562	pobj	%,of
R6286	T7565	T7551	punct	.,fixed
R6287	T7566	T7577	prep	After,added
R6288	T7567	T7568	nummod	10,min
R6289	T7568	T7566	pobj	min,After
R6290	T7569	T7577	punct	",",added
R6291	T7570	T7571	amod	ice-cold,PBS
R6292	T7571	T7577	nsubjpass	PBS,added
R6293	T7572	T7571	cc	plus,PBS
R6294	T7573	T7575	nummod	0.125,glycine
R6295	T7574	T7575	compound	M,glycine
R6296	T7575	T7571	conj	glycine,PBS
R6297	T7576	T7577	auxpass	was,added
R6298	T7577	T7577	ROOT	added,added
R6299	T7578	T7577	punct	",",added
R6300	T7579	T7577	cc	and,added
R6301	T7580	T7582	nsubjpass	plates,transferred
R6302	T7581	T7582	auxpass	were,transferred
R6303	T7582	T7577	conj	transferred,added
R6304	T7583	T7582	prep	on,transferred
R6305	T7584	T7583	pobj	ice,on
R6306	T7585	T7582	punct	",",transferred
R6307	T7586	T7582	conj	washed,transferred
R6308	T7587	T7586	advmod	extensively,washed
R6309	T7588	T7586	prep	with,washed
R6310	T7589	T7588	pobj	PBS,with
R6311	T7590	T7586	punct	",",washed
R6312	T7591	T7586	cc	and,washed
R6313	T7592	T7586	conj	scraped,washed
R6314	T7593	T7577	punct	.,added
R6315	T7594	T7598	prep	After,were
R6316	T7595	T7594	pobj	centrifugation,After
R6317	T7596	T7598	punct	",",were
R6318	T7597	T7598	nsubj	cells,were
R6319	T7598	T7620	ccomp	were,supplemented
R6320	T7599	T7600	nummod	10,min
R6321	T7600	T7598	attr	min,were
R6322	T7601	T7600	amod	lysed,min
R6323	T7602	T7601	prep	in,lysed
R6324	T7603	T7604	compound	lysis,buffer
R6325	T7604	T7602	pobj	buffer,in
R6326	T7605	T7620	punct	(,supplemented
R6327	T7606	T7620	punct	5,supplemented
R6328	T7607	T7609	compound	mM,pH
R6329	T7608	T7609	compound	PIPES,pH
R6330	T7609	T7620	nsubj	pH,supplemented
R6331	T7610	T7609	nummod	8.0,pH
R6332	T7611	T7609	punct	",",pH
R6333	T7612	T7614	nummod	85,KCl
R6334	T7613	T7614	compound	mM,KCl
R6335	T7614	T7609	conj	KCl,pH
R6336	T7615	T7614	punct	",",KCl
R6337	T7616	T7617	nummod	0.5,%
R6338	T7617	T7618	compound	%,NP-40
R6339	T7618	T7614	appos	NP-40,KCl
R6340	T7619	T7620	punct	),supplemented
R6341	T7620	T7620	ROOT	supplemented,supplemented
R6342	T7621	T7620	prep	with,supplemented
R6343	T7622	T7623	nummod	1,×
R6344	T7623	T7626	nummod	×,Inhibitor
R6345	T7624	T7626	amod	Complete,Inhibitor
R6346	T7625	T7626	compound	Protease,Inhibitor
R6347	T7626	T7621	pobj	Inhibitor,with
R6348	T7627	T7630	punct	(,GmbH
R6349	T7628	T7630	compound	Roche,GmbH
R6350	T7629	T7630	compound	Diagnostic,GmbH
R6351	T7630	T7626	appos	GmbH,Inhibitor
R6352	T7631	T7630	punct	),GmbH
R6353	T7632	T7620	punct	.,supplemented
R6354	T7633	T7635	nsubjpass	Nuclei,pelletted
R6355	T7634	T7635	auxpass	were,pelletted
R6356	T7635	T7635	ROOT	pelletted,pelletted
R6357	T7636	T7635	punct	(,pelletted
R6358	T7637	T7638	nummod	1000,×
R6359	T7638	T7639	compound	×,g
R6360	T7639	T7644	dep	g,","
R6361	T7640	T7642	punct	",",min
R6362	T7641	T7642	nummod	5,min
R6363	T7642	T7639	appos	min,g
R6364	T7643	T7639	punct	),g
R6365	T7644	T7635	punct	",",pelletted
R6366	T7645	T7635	cc	and,pelletted
R6367	T7646	T7635	conj	resuspended,pelletted
R6368	T7647	T7646	prep	in,resuspended
R6369	T7648	T7649	compound	sonication,buffer
R6370	T7649	T7647	pobj	buffer,in
R6371	T7650	T7653	punct	(,Tris
R6372	T7651	T7653	nummod	50,Tris
R6373	T7652	T7653	compound	mM,Tris
R6374	T7653	T7646	dep	Tris,resuspended
R6375	T7654	T7655	compound	HCl,pH
R6376	T7655	T7653	appos	pH,Tris
R6377	T7656	T7655	nummod	8.0,pH
R6378	T7657	T7655	punct	",",pH
R6379	T7658	T7659	nummod	1,%
R6380	T7659	T7660	compound	%,SDS
R6381	T7660	T7655	appos	SDS,pH
R6382	T7661	T7660	punct	",",SDS
R6383	T7662	T7664	nummod	10,EDTA
R6384	T7663	T7664	compound	mM,EDTA
R6385	T7664	T7660	appos	EDTA,SDS
R6386	T7665	T7660	punct	),SDS
R6387	T7666	T7653	punct	.,Tris
R6388	T7667	T7669	nsubjpass	Chromatin,sonicated
R6389	T7668	T7669	auxpass	was,sonicated
R6390	T7669	T7669	ROOT	sonicated,sonicated
R6391	T7670	T7669	advcl	using,sonicated
R6392	T7671	T7673	compound	Bandelin,GM70
R6393	T7672	T7673	compound	Sonoplus,GM70
R6394	T7673	T7670	dobj	GM70,using
R6395	T7674	T7673	punct	(,GM70
R6396	T7675	T7676	compound	Bandelin,Electronic
R6397	T7676	T7673	appos	Electronic,GM70
R6398	T7677	T7676	punct	",",Electronic
R6399	T7678	T7676	conj	Berlin,Electronic
R6400	T7679	T7678	punct	",",Berlin
R6401	T7680	T7678	conj	Germany,Berlin
R6402	T7681	T7673	punct	),GM70
R6403	T7682	T7673	punct	",",GM70
R6404	T7683	T7673	amod	centrifuged,GM70
R6405	T7684	T7696	punct	(,was
R6406	T7685	T7686	nummod	14,000
R6407	T7686	T7688	nummod	000,g
R6408	T7687	T7688	compound	×,g
R6409	T7688	T7696	acomp	g,was
R6410	T7689	T7691	punct	",",min
R6411	T7690	T7691	nummod	15,min
R6412	T7691	T7688	appos	min,g
R6413	T7692	T7688	punct	),g
R6414	T7693	T7688	punct	",",g
R6415	T7694	T7688	cc	and,g
R6416	T7695	T7696	nsubj	supernatant,was
R6417	T7696	T7696	ROOT	was,was
R6418	T7697	T7698	advmod	10-fold,diluted
R6419	T7698	T7696	acomp	diluted,was
R6420	T7699	T7698	prep	in,diluted
R6421	T7700	T7701	compound	dilution,buffer
R6422	T7701	T7699	pobj	buffer,in
R6423	T7702	T7705	punct	(,SDS
R6424	T7703	T7704	nummod	0.01,%
R6425	T7704	T7705	npadvmod	%,SDS
R6426	T7705	T7696	parataxis	SDS,was
R6427	T7706	T7705	punct	",",SDS
R6428	T7707	T7709	nummod	16.7,Tris
R6429	T7708	T7709	compound	mM,Tris
R6430	T7709	T7705	conj	Tris,SDS
R6431	T7710	T7711	compound	HCl,pH
R6432	T7711	T7705	npadvmod	pH,SDS
R6433	T7712	T7711	nummod	8.0,pH
R6434	T7713	T7711	punct	",",pH
R6435	T7714	T7715	nummod	1.1,%
R6436	T7715	T7717	compound	%,X-100
R6437	T7716	T7717	compound	Triton,X-100
R6438	T7717	T7711	conj	X-100,pH
R6439	T7718	T7717	punct	",",X-100
R6440	T7719	T7721	nummod	167,NaCl
R6441	T7720	T7721	compound	mM,NaCl
R6442	T7721	T7717	conj	NaCl,X-100
R6443	T7722	T7721	punct	",",NaCl
R6444	T7723	T7725	nummod	1.2,EDTA
R6445	T7724	T7725	compound	mM,EDTA
R6446	T7725	T7721	appos	EDTA,NaCl
R6447	T7726	T7721	punct	),NaCl
R6448	T7727	T7705	punct	.,SDS
R6449	T7728	T7730	nsubjpass	Samples,pre-cleared
R6450	T7729	T7730	auxpass	were,pre-cleared
R6451	T7730	T7730	ROOT	pre-cleared,pre-cleared
R6452	T7731	T7730	agent	by,pre-cleared
R6453	T7732	T7733	amod	3-h,incubation
R6454	T7733	T7731	pobj	incubation,by
R6455	T7734	T7730	prep	with,pre-cleared
R6456	T7735	T7736	nummod	20,µl
R6457	T7736	T7734	pobj	µl,with
R6458	T7737	T7736	prep	of,µl
R6459	T7738	T7741	nmod	protein,beads
R6460	T7739	T7741	nmod	G,beads
R6461	T7740	T7741	amod	agarose,beads
R6462	T7741	T7737	pobj	beads,of
R6463	T7742	T7741	acl	followed,beads
R6464	T7743	T7742	agent	by,followed
R6465	T7744	T7743	pobj	incubation,by
R6466	T7745	T7742	prep	with,followed
R6467	T7746	T7745	pobj	antibodies,with
R6468	T7747	T7746	prep	against,antibodies
R6469	T7748	T7750	det	the,proteins
R6470	T7749	T7750	amod	analysed,proteins
R6471	T7750	T7747	pobj	proteins,against
R6472	T7751	T7730	punct	.,pre-cleared
R6473	T7752	T7753	amod	Primary,antibodies
R6474	T7753	T7754	nsubj	antibodies,were
R6475	T7754	T7754	ROOT	were,were
R6476	T7755	T7754	punct	:,were
R6477	T7756	T7767	amod	anti-p65,IgG
R6478	T7757	T7767	punct	(,IgG
R6479	T7758	T7767	amod	sc-372,IgG
R6480	T7759	T7758	punct	),sc-372
R6481	T7760	T7758	punct	",",sc-372
R6482	T7761	T7758	conj	anti-IκBα,sc-372
R6483	T7762	T7763	punct	(,sc-203
R6484	T7763	T7767	amod	sc-203,IgG
R6485	T7764	T7763	punct	),sc-203
R6486	T7765	T7763	cc	and,sc-203
R6487	T7766	T7763	conj	rabbit,sc-203
R6488	T7767	T7754	attr	IgG,were
R6489	T7768	T7769	punct	(,sc-2027
R6490	T7769	T7767	appos	sc-2027,IgG
R6491	T7770	T7769	punct	),sc-2027
R6492	T7771	T7769	prep	from,sc-2027
R6493	T7772	T7773	compound	Santa,Cruz
R6494	T7773	T7774	compound	Cruz,Biotechnology
R6495	T7774	T7771	pobj	Biotechnology,from
R6496	T7775	T7767	punct	;,IgG
R6497	T7776	T7777	compound	anti-FLAG,M2
R6498	T7777	T7767	conj	M2,IgG
R6499	T7778	T7767	prep	from,IgG
R6500	T7779	T7778	pobj	Sigma,from
R6501	T7780	T7767	conj	Aldrich,IgG
R6502	T7781	T7767	punct	.,IgG
R6503	T7782	T7784	nsubjpass	Immunoprecipitations,carried
R6504	T7783	T7784	auxpass	were,carried
R6505	T7784	T7784	ROOT	carried,carried
R6506	T7785	T7784	prt	out,carried
R6507	T7786	T7784	prep	at,carried
R6508	T7787	T7788	nummod	4,°
R6509	T7788	T7790	nummod	°,overnight
R6510	T7789	T7790	compound	C,overnight
R6511	T7790	T7786	pobj	overnight,at
R6512	T7791	T7790	cc	and,overnight
R6513	T7792	T7790	conj	immune,overnight
R6514	T7793	T7795	nsubjpass	complexes,collected
R6515	T7794	T7795	auxpass	were,collected
R6516	T7795	T7784	conj	collected,carried
R6517	T7796	T7795	prep	with,collected
R6518	T7797	T7800	nmod	protein,beads
R6519	T7798	T7800	nmod	G,beads
R6520	T7799	T7800	amod	agarose,beads
R6521	T7800	T7796	pobj	beads,with
R6522	T7801	T7802	punct	",",washed
R6523	T7802	T7784	conj	washed,carried
R6524	T7803	T7804	nummod	five,times
R6525	T7804	T7802	npadvmod	times,washed
R6526	T7805	T7802	prep	with,washed
R6527	T7806	T7808	amod	low,buffer
R6528	T7807	T7808	compound	salt,buffer
R6529	T7808	T7805	pobj	buffer,with
R6530	T7809	T7808	punct	(,buffer
R6531	T7810	T7812	nummod	20,Tris
R6532	T7811	T7812	compound	mM,Tris
R6533	T7812	T7808	appos	Tris,buffer
R6534	T7813	T7814	compound	HCl,pH
R6535	T7814	T7808	appos	pH,buffer
R6536	T7815	T7814	nummod	8.0,pH
R6537	T7816	T7814	punct	",",pH
R6538	T7817	T7818	nummod	0.1,%
R6539	T7818	T7819	npadvmod	%,SDS
R6540	T7819	T7814	appos	SDS,pH
R6541	T7820	T7819	punct	",",SDS
R6542	T7821	T7822	nummod	1,%
R6543	T7822	T7824	compound	%,X-100
R6544	T7823	T7824	compound	Triton,X-100
R6545	T7824	T7819	conj	X-100,SDS
R6546	T7825	T7824	punct	",",X-100
R6547	T7826	T7828	nummod	2,EDTA
R6548	T7827	T7828	compound	mM,EDTA
R6549	T7828	T7824	conj	EDTA,X-100
R6550	T7829	T7828	punct	",",EDTA
R6551	T7830	T7832	nummod	150,NaCl
R6552	T7831	T7832	compound	mM,NaCl
R6553	T7832	T7828	appos	NaCl,EDTA
R6554	T7833	T7828	punct	),EDTA
R6555	T7834	T7819	punct	",",SDS
R6556	T7835	T7836	nummod	four,times
R6557	T7836	T7819	npadvmod	times,SDS
R6558	T7837	T7819	prep	with,SDS
R6559	T7838	T7840	amod	high,buffer
R6560	T7839	T7840	compound	salt,buffer
R6561	T7840	T7837	pobj	buffer,with
R6562	T7841	T7844	punct	(,Tris
R6563	T7842	T7844	nummod	20,Tris
R6564	T7843	T7844	compound	mM,Tris
R6565	T7844	T7840	appos	Tris,buffer
R6566	T7845	T7846	compound	HCl,pH
R6567	T7846	T7885	nsubj	pH,extracted
R6568	T7847	T7846	nummod	8.0,pH
R6569	T7848	T7846	punct	",",pH
R6570	T7849	T7850	nummod	0.1,%
R6571	T7850	T7851	npadvmod	%,SDS
R6572	T7851	T7846	npadvmod	SDS,pH
R6573	T7852	T7851	punct	",",SDS
R6574	T7853	T7854	nummod	1,%
R6575	T7854	T7856	compound	%,X-100
R6576	T7855	T7856	compound	Triton,X-100
R6577	T7856	T7851	appos	X-100,SDS
R6578	T7857	T7856	punct	",",X-100
R6579	T7858	T7860	nummod	2,EDTA
R6580	T7859	T7860	compound	mM,EDTA
R6581	T7860	T7856	conj	EDTA,X-100
R6582	T7861	T7860	punct	",",EDTA
R6583	T7862	T7864	nummod	500,NaCl
R6584	T7863	T7864	compound	mM,NaCl
R6585	T7864	T7860	appos	NaCl,EDTA
R6586	T7865	T7860	punct	),EDTA
R6587	T7866	T7851	punct	",",SDS
R6588	T7867	T7868	advmod	once,with
R6589	T7868	T7851	prep	with,SDS
R6590	T7869	T7870	compound	TE,buffer
R6591	T7870	T7868	pobj	buffer,with
R6592	T7871	T7851	punct	(,SDS
R6593	T7872	T7874	nummod	10,Tris
R6594	T7873	T7874	compound	mM,Tris
R6595	T7874	T7851	appos	Tris,SDS
R6596	T7875	T7876	compound	HCl,pH
R6597	T7876	T7851	appos	pH,SDS
R6598	T7877	T7876	nummod	8.0,pH
R6599	T7878	T7876	punct	",",pH
R6600	T7879	T7881	nummod	1,EDTA
R6601	T7880	T7881	compound	mM,EDTA
R6602	T7881	T7876	appos	EDTA,pH
R6603	T7882	T7876	punct	),pH
R6604	T7883	T7851	punct	",",SDS
R6605	T7884	T7846	cc	and,pH
R6606	T7885	T7885	ROOT	extracted,extracted
R6607	T7886	T7885	prep	in,extracted
R6608	T7887	T7888	compound	TE,buffer
R6609	T7888	T7886	pobj	buffer,in
R6610	T7889	T7888	acl	containing,buffer
R6611	T7890	T7891	nummod	2,%
R6612	T7891	T7889	dobj	%,containing
R6613	T7892	T7889	npadvmod	SDS,containing
R6614	T7893	T7885	punct	.,extracted
R6615	T7894	T7894	ROOT	Protein,Protein
R6616	T7895	T7896	compound	DNA,cross-links
R6617	T7896	T7898	nsubjpass	cross-links,reverted
R6618	T7897	T7898	auxpass	were,reverted
R6619	T7898	T7898	ROOT	reverted,reverted
R6620	T7899	T7898	agent	by,reverted
R6621	T7900	T7899	pobj	heating,by
R6622	T7901	T7898	prep	at,reverted
R6623	T7902	T7904	nummod	65,C
R6624	T7903	T7904	nummod	°,C
R6625	T7904	T7905	compound	C,overnight
R6626	T7905	T7901	pobj	overnight,at
R6627	T7906	T7898	punct	.,reverted
R6628	T7907	T7910	nsubjpass	DNA,purified
R6629	T7908	T7910	auxpass	was,purified
R6630	T7909	T7910	advmod	further,purified
R6631	T7910	T7910	ROOT	purified,purified
R6650	T7929	T7935	nsubjpass	enrichment,measured
R6651	T7930	T7929	prep	in,enrichment
R6652	T7931	T7933	amod	NF-κB,sequences
R6653	T7932	T7933	compound	enhancer,sequences
R6654	T7933	T7930	pobj	sequences,in
R6655	T7934	T7935	auxpass	was,measured
R6656	T7935	T7935	ROOT	measured,measured
R6657	T7936	T7935	agent	by,measured
R6658	T7937	T7938	amod	real-time,PCR
R6659	T7938	T7936	pobj	PCR,by
R6660	T7939	T7938	prep	of,PCR
R6661	T7940	T7941	compound	chromatin,immunoprecipitation
R6662	T7941	T7939	pobj	immunoprecipitation,of
R6663	T7942	T7943	punct	(,ChIP
R6664	T7943	T7941	appos	ChIP,immunoprecipitation
R6665	T7944	T7941	punct	),immunoprecipitation
R6666	T7945	T7935	oprd	eluates,measured
R6667	T7946	T7945	advcl	using,eluates
R6668	T7947	T7950	compound	SYBR,Mix
R6669	T7948	T7950	compound	GreenER,Mix
R6670	T7949	T7950	compound	Master,Mix
R6671	T7950	T7946	dobj	Mix,using
R6672	T7951	T7950	punct	(,Mix
R6673	T7952	T7950	appos	Invitrogen,Mix
R6674	T7953	T7950	punct	),Mix
R6675	T7954	T7935	punct	.,measured
R6676	T7955	T7957	nsubjpass	Reactions,carried
R6677	T7956	T7957	auxpass	were,carried
R6678	T7957	T7957	ROOT	carried,carried
R6679	T7958	T7957	prt	out,carried
R6680	T7959	T7957	prep	with,carried
R6681	T7960	T7965	det	the,system
R6682	T7961	T7963	compound	iCycler,Real-Time
R6683	T7962	T7963	compound	iQ,Real-Time
R6684	T7963	T7965	compound	Real-Time,system
R6685	T7964	T7965	compound	detection,system
R6686	T7965	T7959	pobj	system,with
R6687	T7966	T7965	punct	(,system
R6688	T7967	T7968	compound	Bio-Rad,Laboratories
R6689	T7968	T7965	appos	Laboratories,system
R6690	T7969	T7965	punct	),system
R6691	T7970	T7957	advcl	using,carried
R6692	T7971	T7973	det	the,conditions
R6693	T7972	T7973	amod	following,conditions
R6694	T7973	T7970	dobj	conditions,using
R6695	T7974	T7973	punct	:,conditions
R6696	T7975	T7976	nummod	95,°
R6697	T7976	T7977	compound	°,C
R6698	T7977	T7973	appos	C,conditions
R6699	T7978	T7977	punct	",",C
R6700	T7979	T7980	nummod	1,min
R6701	T7980	T7977	appos	min,C
R6702	T7981	T7977	punct	;,C
R6703	T7982	T7985	punct	(,C
R6704	T7983	T7984	nummod	94,°
R6705	T7984	T7985	compound	°,C
R6706	T7985	T7977	appos	C,C
R6707	T7986	T7985	punct	",",C
R6708	T7987	T7988	nsubj	10,s
R6709	T7988	T7977	appos	s,C
R6710	T7989	T7977	punct	;,C
R6711	T7990	T7991	nummod	60,°
R6712	T7991	T7992	compound	°,C
R6713	T7992	T7977	conj	C,C
R6714	T7993	T7992	punct	",",C
R6715	T7994	T7995	nummod	30,s
R6716	T7995	T7992	appos	s,C
R6717	T7996	T7992	punct	),C
R6718	T7997	T7998	advmod	×,40
R6719	T7998	T7977	appos	40,C
R6720	T7999	T7957	punct	.,carried
R6721	T8000	T8009	nsubjpass	Primers,listed
R6722	T8001	T8000	acl	used,Primers
R6723	T8002	T8001	prep	for,used
R6724	T8003	T8002	pobj	MIP-1α,for
R6725	T8004	T8003	punct	",",MIP-1α
R6726	T8005	T8003	conj	GAPDH,MIP-1α
R6727	T8006	T8005	cc	and,GAPDH
R6728	T8007	T8005	conj	ACTB,GAPDH
R6729	T8008	T8009	auxpass	are,listed
R6730	T8009	T8009	ROOT	listed,listed
R6731	T8010	T8009	prep	in,listed
R6732	T8011	T8012	compound	Supplementary,Data
R6733	T8012	T8010	pobj	Data,in
R6734	T8013	T8009	punct	.,listed
R6735	T8014	T8015	amod	Real-time,PCR
R6736	T8015	T8025	nsubjpass	PCR,performed
R6737	T8016	T8015	prep	of,PCR
R6738	T8017	T8016	pobj	CSF3,of
R6739	T8018	T8017	punct	",",CSF3
R6740	T8019	T8017	conj	LTA,CSF3
R6741	T8020	T8019	punct	",",LTA
R6742	T8021	T8019	conj	NFKBIA,LTA
R6743	T8022	T8021	cc	and,NFKBIA
R6744	T8023	T8021	conj	TLR2,NFKBIA
R6745	T8024	T8025	auxpass	were,performed
R6746	T8025	T8025	ROOT	performed,performed
R6747	T8026	T8025	advcl	using,performed
R6748	T8027	T8030	det	the,array
R6749	T8028	T8030	compound	Custom,array
R6750	T8029	T8030	compound	ChIP,array
R6751	T8030	T8026	dobj	array,using
R6752	T8031	T8030	punct	(,array
R6753	T8032	T8030	appos	QIAGEN,array
R6754	T8033	T8030	punct	),array
R6755	T8034	T8025	punct	.,performed
R6756	T8035	T8040	prep	For,were
R6757	T8036	T8037	det	each,sample
R6758	T8037	T8035	pobj	sample,For
R6759	T8038	T8040	punct	",",were
R6760	T8039	T8040	nsubj	values,were
R6761	T8040	T8040	ROOT	were,were
R6762	T8041	T8040	acomp	normalized,were
R6763	T8042	T8041	prep	to,normalized
R6764	T8043	T8046	dep	input,reported
R6765	T8044	T8043	dobj	DNA,input
R6766	T8045	T8043	cc	and,input
R6767	T8046	T8040	conj	reported,were
R6768	T8047	T8048	mark	as,%
R6769	T8048	T8046	advcl	%,reported
R6770	T8049	T8048	prep	of,%
R6771	T8050	T8049	pobj	input,of
R6772	T8051	T8048	prep	over,%
R6773	T8052	T8053	det	the,rabbit
R6774	T8053	T8051	pobj	rabbit,over
R6775	T8054	T8055	compound	IgG,control
R6776	T8055	T8048	conj	control,%
R6777	T8056	T8046	punct	.,reported
R6815	T8195	T8196	compound	Statistical,analysis
R6816	T8196	T8198	compound	analysis,analysis
R6817	T8197	T8198	compound	Statistical,analysis
R6818	T8198	T8200	nsubjpass	analysis,performed
R6819	T8199	T8200	auxpass	was,performed
R6820	T8200	T8200	ROOT	performed,performed
R6821	T8201	T8200	agent	by,performed
R6822	T8202	T8204	compound	two-tail,Student
R6823	T8203	T8204	compound	unpaired,Student
R6824	T8204	T8201	pobj	Student,by
R6825	T8205	T8204	case	's,Student
R6826	T8206	T8204	amod	t-test,Student
R6827	T8207	T8200	punct	.,performed
R6828	T8208	T8210	nsubjpass	Data,reported
R6829	T8209	T8210	auxpass	were,reported
R6830	T8210	T8210	ROOT	reported,reported
R6831	T8211	T8210	prep	as,reported
R6832	T8212	T8211	pobj	means,as
R6833	T8213	T8214	compound	±,SE
R6834	T8214	T8212	dobj	SE,means
R6835	T8215	T8210	punct	.,reported
R6836	T8216	T8221	nsubjpass	Differences,considered
R6837	T8217	T8216	prep	between,Differences
R6838	T8218	T8219	det	the,means
R6839	T8219	T8217	pobj	means,between
R6840	T8220	T8221	auxpass	were,considered
R6841	T8221	T8221	ROOT	considered,considered
R6842	T8222	T8221	prep	as,considered
R6843	T8223	T8224	advmod	statistically,significant
R6844	T8224	T8222	amod	significant,as
R6845	T8225	T8224	prep	at,significant
R6846	T8226	T8229	det	the,level
R6847	T8227	T8228	nummod	95,%
R6848	T8228	T8229	compound	%,level
R6849	T8229	T8225	pobj	level,at
R6850	T8230	T8229	punct	(,level
R6851	T8231	T8234	nmod	P,)
R6852	T8232	T8233	compound	<,0.05
R6853	T8233	T8231	nummod	0.05,P
R6854	T8234	T8229	punct	),level
R6855	T8235	T8221	punct	.,considered
R4218	T4664	T4662	conj	NIH,NIAID
R4219	T4665	T4662	punct	",",NIAID
R4220	T4666	T4662	conj	USA,NIAID
R4221	T4667	T4672	punct	;,purchased
R4222	T4668	T4672	nsubjpass	pκBluc,purchased
R4223	T4669	T4668	cc	and,pκBluc
R5134	T5930	T5931	compound	CUGCUUGUCACA,AUUGCUAUU
R5596	T6545	T6543	appos	Danvers,Technology
R6130	T7260	T7261	compound	Bio-Rad,Laboratories
R6131	T7261	T7258	appos	Laboratories,mix
R6132	T7262	T7252	punct	),performed
R6133	T7263	T7252	cc	and,performed
R6134	T7264	T7252	conj	carried,performed
R6135	T7265	T7264	prt	out,carried
R6136	T7266	T7264	prep	with,carried
R6137	T7267	T7272	det	the,system
R6138	T7268	T7270	compound	iCycler,Real-Time
R6139	T7269	T7270	compound	iQ,Real-Time
R6140	T7270	T7272	compound	Real-Time,system
R6141	T7271	T7272	compound	detection,system
R6142	T7272	T7266	pobj	system,with
R6143	T7273	T7272	punct	(,system
R6144	T7274	T7275	compound	Bio-Rad,Laboratories
R6145	T7275	T7272	appos	Laboratories,system
R6146	T7276	T7272	punct	),system
R6147	T7277	T7264	prep	under,carried
R6148	T7278	T7280	det	the,conditions
R6149	T7279	T7280	amod	following,conditions
R6150	T7280	T7277	pobj	conditions,under
R6151	T7281	T7280	punct	:,conditions
R6152	T7282	T7283	nummod	95,°
R6153	T7283	T7284	compound	°,C
R6154	T7284	T7280	appos	C,conditions
R6155	T7285	T7284	punct	",",C
R6156	T7286	T7287	nummod	1,min
R6157	T7287	T7284	appos	min,C
R6158	T7288	T7284	punct	;,C
R6159	T7289	T7284	punct	(,C
R6160	T7290	T7291	nummod	94,°
R6632	T7911	T7910	agent	by,purified
R6633	T7912	T7915	compound	QIAquick,kit
R6634	T7913	T7915	compound	PCR,kit
R6635	T7914	T7915	compound	purification,kit
R6636	T7915	T7911	pobj	kit,by
R6637	T7916	T7915	punct	(,kit
R6638	T7917	T7915	appos	QIAGEN,kit
R6639	T7918	T7915	punct	),kit
R6640	T7919	T7910	cc	and,purified
R6641	T7920	T7910	conj	eluted,purified
R6642	T7921	T7920	prep	in,eluted
R6643	T7922	T7923	nummod	50,µl
R6644	T7923	T7924	npadvmod	µl,sterile
R6645	T7924	T7926	amod	sterile,water
R6646	T7925	T7926	compound	distilled,water
R6647	T7926	T7921	pobj	water,in
R6648	T7927	T7910	punct	.,purified
R6649	T7928	T7929	amod	Specific,enrichment
R4211	T4657	T4656	punct	",",Program

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T4890	957-962	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T4891	1128-1133	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T5668	2504-2509	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T6281	4211-4215	http://purl.obolibrary.org/obo/UBERON_0001913	denotes	milk
T7505	6768-6779	http://purl.obolibrary.org/obo/UBERON_2000106	denotes	extensively
T8154	8986-8990	http://purl.obolibrary.org/obo/UBERON_0002415	denotes	tail

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T5503	1444-1446	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T5504	2137-2139	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T5505	1609-1617	http://purl.obolibrary.org/obo/GO_0001906	denotes	necrosis
T5506	1609-1617	http://purl.obolibrary.org/obo/GO_0008219	denotes	necrosis
T5507	1609-1617	http://purl.obolibrary.org/obo/GO_0019835	denotes	necrosis
T5508	1903-1908	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T5509	1609-1617	http://purl.obolibrary.org/obo/GO_0070265	denotes	necrosis
T5510	1998-2024	http://purl.obolibrary.org/obo/GO_0004565	denotes	β-galactosidase activities
T5511	2179-2206	http://purl.obolibrary.org/obo/GO_0004565	denotes	activity to β-galactosidase
T5512	1998-2024	http://purl.obolibrary.org/obo/GO_0004336	denotes	β-galactosidase activities
T5513	2179-2206	http://purl.obolibrary.org/obo/GO_0004336	denotes	activity to β-galactosidase
T5514	1998-2024	http://purl.obolibrary.org/obo/GO_1903771	denotes	β-galactosidase activities
T5515	2179-2206	http://purl.obolibrary.org/obo/GO_1903771	denotes	activity to β-galactosidase
T5516	1998-2024	http://purl.obolibrary.org/obo/GO_1903770	denotes	β-galactosidase activities
T5517	2179-2206	http://purl.obolibrary.org/obo/GO_1903770	denotes	activity to β-galactosidase
T5518	1998-2024	http://purl.obolibrary.org/obo/GO_0033920	denotes	β-galactosidase activities
T5519	2179-2206	http://purl.obolibrary.org/obo/GO_0033920	denotes	activity to β-galactosidase
T5520	1998-2024	http://purl.obolibrary.org/obo/GO_0033929	denotes	β-galactosidase activities
T5521	2179-2206	http://purl.obolibrary.org/obo/GO_0033929	denotes	activity to β-galactosidase
T5522	1998-2024	http://purl.obolibrary.org/obo/GO_0033930	denotes	β-galactosidase activities
T5523	2179-2206	http://purl.obolibrary.org/obo/GO_0033930	denotes	activity to β-galactosidase
T5524	2168-2187	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T5525	2168-2187	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T5526	2168-2187	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T5527	2168-2187	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T5528	2168-2187	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T5972	2256-2272	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNA interference
T5973	2682-2698	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNA interference
T5974	2902-2918	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNA interference
T5975	2389-2391	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T6618	3529-3532	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6619	4653-4656	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6620	4719-4722	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6621	4409-4411	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T6622	4896-4909	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T6811	5117-5128	http://purl.obolibrary.org/obo/GO_0006412	denotes	translation
T6812	5254-5265	http://purl.obolibrary.org/obo/GO_0006412	denotes	translation
T6813	5240-5253	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T7397	5787-5800	http://purl.obolibrary.org/obo/GO_0003899	denotes	Transcriptase
T7398	5787-5800	http://purl.obolibrary.org/obo/GO_0003968	denotes	Transcriptase
T7399	5787-5800	http://purl.obolibrary.org/obo/GO_0034062	denotes	Transcriptase
T7400	6274-6283	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T7401	6274-6291	http://purl.obolibrary.org/obo/GO_0007165	denotes	signaling pathway
T7402	6366-6381	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T8080	6852-6857	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T5529	1444-1446	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T5530	2137-2139	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T5531	1998-2024	http://purl.obolibrary.org/obo/GO_0004565	denotes	β-galactosidase activities
T5532	2179-2206	http://purl.obolibrary.org/obo/GO_0004565	denotes	activity to β-galactosidase
T5533	1998-2024	http://purl.obolibrary.org/obo/GO_0004336	denotes	β-galactosidase activities
T5534	2179-2206	http://purl.obolibrary.org/obo/GO_0004336	denotes	activity to β-galactosidase
T5535	1998-2024	http://purl.obolibrary.org/obo/GO_0033920	denotes	β-galactosidase activities
T5536	2179-2206	http://purl.obolibrary.org/obo/GO_0033920	denotes	activity to β-galactosidase
T5537	1998-2024	http://purl.obolibrary.org/obo/GO_0033929	denotes	β-galactosidase activities
T5538	2179-2206	http://purl.obolibrary.org/obo/GO_0033929	denotes	activity to β-galactosidase
T5539	1998-2024	http://purl.obolibrary.org/obo/GO_0033930	denotes	β-galactosidase activities
T5540	2179-2206	http://purl.obolibrary.org/obo/GO_0033930	denotes	activity to β-galactosidase
T5541	2168-2187	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T5542	2168-2187	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T5543	2168-2187	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T5544	2168-2187	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T5545	2168-2187	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T5976	2389-2391	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T6238	3305-3313	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T6623	3529-3532	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6624	4653-4656	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6625	4719-4722	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T6626	3552-3563	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T6627	3556-3563	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T6628	4787-4794	http://purl.obolibrary.org/obo/GO_0005488	denotes	Binding
T6629	4008-4018	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T6630	4339-4349	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T6631	4409-4411	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T7403	5787-5800	http://purl.obolibrary.org/obo/GO_0003899	denotes	Transcriptase
T7404	5787-5800	http://purl.obolibrary.org/obo/GO_0003968	denotes	Transcriptase
T7405	5787-5800	http://purl.obolibrary.org/obo/GO_0034062	denotes	Transcriptase
T8081	7479-7489	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T8082	7529-7539	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T5546	858-863	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5547	930-935	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5548	975-980	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5549	1482-1487	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5550	1883-1888	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6239	3030-3037	http://purl.obolibrary.org/obo/GO_0019012	denotes	virions
T6240	3423-3430	http://purl.obolibrary.org/obo/GO_0019012	denotes	virions
T6241	3070-3075	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6242	3367-3372	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6243	3305-3313	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T6632	3496-3500	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T6633	4741-4745	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T6634	3942-3950	http://purl.obolibrary.org/obo/GO_0016020	denotes	membrane
T6635	4008-4018	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T6636	4339-4349	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T6637	4008-4018	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T6638	4339-4349	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T7406	5635-5640	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5969	2288-2293	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5970	2426-2431	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5971	2631-2636	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6237	3305-3313	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T8072	6540-6549	http://purl.obolibrary.org/obo/GO_0000785	denotes	Chromatin
T8073	7105-7114	http://purl.obolibrary.org/obo/GO_0000785	denotes	Chromatin
T8074	8381-8390	http://purl.obolibrary.org/obo/GO_0000785	denotes	chromatin
T8075	6825-6830	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T8076	7479-7489	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T8077	7529-7539	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T8078	7479-7489	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T8079	7529-7539	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies

sentences

Id	Subject	Object	Predicate	Lexical cue
T4409	23-31	Sentence	denotes	Plasmids
T4410	32-256	Sentence	denotes	The plasmids pcDNA-3xHA-IκB-α, p3xFLAG-CMV-Tat, p3xFLAG-CMV-Tat C(22,25,27)A, p3xFLAG-CMV-Tat R(49,52,53,55,56,57)A, pGEX-2T-Tat, pGEX-2T-Tat C(22,25,27)A and pGEX-2T-Tat R(49,52,53,55,56,57)A were previously described (50).
T4411	257-469	Sentence	denotes	The plasmids pNL4-3.Luc.R-E- and pHXB2-env were obtained from the AIDS Research & Reference Reagent Program, Division of AIDS, NIAID, NIH, USA; pκBluc and pSV-β-Gal were purchased from Promega (Madison, WI, USA).
T4412	470-735	Sentence	denotes	The plasmids pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC(1–318), pRc/CMV-3xHA-p65ΔN(122–551), p3xFLAG-CMV-Tat T,N(23,24)A, p3xFLAG-CMV-Tat K(50,51)A, pGEX-2T-Tat T,N(23,24)A, pGEX-2T-Tat K(50,51)A and pNL4-3.FLAG-Tat.R-E- were generated as described in Supplementary Data.
T4906	737-789	Sentence	denotes	Cells, transfection, treatments and luciferase assay
T4907	790-1016	Sentence	denotes	HeLa, p50−/−p65−/− mouse embryonic fibroblasts (MEFs) (52) and 293T cells were cultured in Dulbecco's modified Eagle's medium; Jurkat, U937 cells and human peripheral blood mononuclear cells (PBMCs) were cultured in RPMI 1640.
T4908	1017-1066	Sentence	denotes	PBMCs were isolated as previously described (53).
T4909	1067-1183	Sentence	denotes	Media were supplemented with 10% heat-inactivated fetal calf serum and 2 mM l-glutamine (Lonza Cologne AG, Germany).
T4910	1184-1453	Sentence	denotes	HeLa, p50−/−p65−/− MEFs and 293T were transfected with DNA by using FuGENE HD (Roche Diagnostic GmbH, Mannheim, Germany), according to the manufacturer's protocol; total DNA amounts were equalized by transfection of pRc/CMV empty vector (Invitrogen, Carlsbad, CA, USA).
T4911	1454-1742	Sentence	denotes	For pulse-stimulation, HeLa cells were treated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St Louis, MO, USA) (20 ng/ml) for 5 min, or tumor necrosis factor-α (TNF-α; Sigma-Aldrich) (20 ng/ml) for 30 min, washed twice in complete culture medium and then returned to culture.
T4912	1743-1846	Sentence	denotes	For luciferase assays, pSV-β-Gal was co-transfected with pκBluc to monitor the transfection efficiency.
T4913	1847-2146	Sentence	denotes	Forty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA).
T4914	2147-2254	Sentence	denotes	The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.
T5677	2256-2272	Sentence	denotes	RNA interference
T5678	2273-2398	Sentence	denotes	Jurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA).
T5679	2399-2681	Sentence	denotes	Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium.
T5680	2682-2901	Sentence	denotes	RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU.
T5681	2902-3016	Sentence	denotes	RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA).
T6045	3018-3064	Sentence	denotes	Pseudotyped virions and single round infection
T6046	3065-3247	Sentence	denotes	293T cells (1 × 107) were transfected with pNL4-3.Luc.R-E- or pNL4-3.FLAG-Tat.R-E- (10 µg) together with pHXB2 Env (10 µg), and 48-h post-transfection cell supernatant was collected.
T6047	3248-3344	Sentence	denotes	Enzyme-linked immunosorbent assay (ELISA) using anti-p24 antibody measured virion concentration.
T6048	3345-3494	Sentence	denotes	PBMCs, Jurkat or U937 cells (5 × 107) were infected with HXB2 Env-pseudotyped virions (500 ng of p24) by spinoculation, as previously described (50).
T6288	3496-3563	Sentence	denotes	Cell extracts, western blotting, IKK activity and NF-κB DNA binding
T6289	3564-4239	Sentence	denotes	Total, nuclear and cytosolic extracts were performed as previously described (54); details are reported in Supplementary Data. Western blotting analysis was performed by resuspending protein aliquots in loading buffer (125 mM Tris–HCl, pH 6.8, 5% SDS, 1% bromophenol blue, 10% β-mercaptoethanol, 25% glycerol), resolved on 12% SDS–PAGE, transferred to polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA) and incubated with primary antibodies (1:1000) followed by incubation with horseradish-peroxidase-linked mouse or rabbit IgG (1:2000) (GE Healthcare Amersham, Little Chalfont, Buckinghamshire, UK) in PBS containing 5% non-fat dry milk (Bio-Rad Laboratories).
T6290	4240-4330	Sentence	denotes	Proteins were detected by chemiluminescence using the ECL System (GE Healthcare Amersham).
T6291	4331-4370	Sentence	denotes	Primary antibodies were purchased from:
T6292	4371-4571	Sentence	denotes	Santa Cruz Biotechnology, Santa Cruz, CA, USA (anti-HA F7, anti-IκB-α C15, anti-Histone H1, anti-Hexokinase-II); Sigma-Aldrich (anti-FLAG M2, anti-γ-Tubulin); Upstate, Lake Placid, NY, USA (anti-p65).
T6293	4572-4652	Sentence	denotes	Densitometry of single bands was analysed by ImageJ software package (NIH, USA).
T6294	4653-4786	Sentence	denotes	IKK activity was evaluated in cytosolic extracts using the HTScan IKK kinase assay kit (Cell Signaling Technology, Danvers, MA, USA).
T6295	4787-4973	Sentence	denotes	Binding of p65, p50 and FLAG-Tat to the double-stranded NF-κB oligonucleotide was measured using NF-κB Combo Transcription Factor Assay kit (Cayman Chemical Company, Ann Arbor, MI, USA).
T6296	4974-5106	Sentence	denotes	Electrophoretic Mobility Shift Assay (EMSA) was performed as previously described (55); details are described in Supplementary Data.
T6715	5108-5128	Sentence	denotes	In vitro translation
T6716	5129-5312	Sentence	denotes	HA-IκB-α, p65 and Tat were expressed under the T7 promoter and in vitro translated using the TnT quick coupled transcription/translation system (Promega), as previously reported (50).
T6717	5313-5357	Sentence	denotes	Details are described in Supplementary Data.
T6879	5359-5402	Sentence	denotes	Immunoprecipitation assay and GST-pull down
T6880	5403-5545	Sentence	denotes	Immunoprecipitation, GST-pull down, and production of GST proteins in Escherichia coli strain BL21 were performed as previously reported (50).
T6881	5546-5590	Sentence	denotes	Details are described in Supplementary Data.
T7009	5592-5605	Sentence	denotes	Real-time PCR
T7010	5606-5856	Sentence	denotes	Total RNA was extracted from cells by using the TRIzol reagent (Invitrogen); RNA aliquots (200 ng) were reverse transcribed using Random Examers (Roche) and Superscript III Reverse Transcriptase (Invitrogen), according to the manufacturer's protocol.
T7011	5857-6053	Sentence	denotes	Real-time PCR was performed with the iQ Green Super mix (Bio-Rad Laboratories) and carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) under the following conditions:
T7012	6054-6096	Sentence	denotes	95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T7013	6097-6319	Sentence	denotes	Primers for Tat and MIP-1α are listed in Supplementary Data. Real-time PCR of CSF3, LTA, NFKBIA, TLR2, GAPDH and ACTB was performed using the RT2 profiler PCR Array-Human NF-κB signaling pathway (QIAGEN Sciences, MD, USA).
T7014	6320-6457	Sentence	denotes	Reactions were carried out in triplicate, and gene expression levels were calculated relative to GAPDH mRNA levels as endogenous control.
T7015	6458-6538	Sentence	denotes	Relative expression was calculated as 2(Ct gene under investigation − Ct GAPDH).
T7515	6540-6575	Sentence	denotes	Chromatin immunoprecipitation assay
T7516	6576-6665	Sentence	denotes	Cells were fixed by adding formaldehyde (Sigma-Aldrich) at the final concentration of 1%.
T7517	6666-6802	Sentence	denotes	After 10 min, ice-cold PBS plus 0.125 M glycine was added, and plates were transferred on ice, washed extensively with PBS, and scraped.
T7518	6803-6981	Sentence	denotes	After centrifugation, cells were 10 min lysed in lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40) supplemented with 1× Complete Protease Inhibitor (Roche Diagnostic GmbH).
T7519	6982-7104	Sentence	denotes	Nuclei were pelletted (1000 × g, 5 min), and resuspended in sonication buffer (50 mM Tris–HCl pH 8.0, 1% SDS, 10 mM EDTA).
T7520	7105-7369	Sentence	denotes	Chromatin was sonicated using Bandelin Sonoplus GM70 (Bandelin Electronic, Berlin, Germany), centrifuged (14 000 × g, 15 min), and supernatant was 10-fold diluted in dilution buffer (0.01% SDS, 16.7 mM Tris–HCl pH 8.0, 1.1% Triton X-100, 167 mM NaCl, 1.2 mM EDTA).
T7521	7370-7520	Sentence	denotes	Samples were pre-cleared by 3-h incubation with 20 µl of protein G agarose beads followed by incubation with antibodies against the analysed proteins.
T7522	7521-8119	Sentence	denotes	Primary antibodies were: anti-p65 (sc-372), anti-IκBα (sc-203) and rabbit IgG (sc-2027) from Santa Cruz Biotechnology; anti-FLAG M2 from Sigma–Aldrich. Immunoprecipitations were carried out at 4°C overnight and immune complexes were collected with protein G agarose beads, washed five times with low salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl), four times with high salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 500 mM NaCl), once with TE buffer (10 mM Tris–HCl pH 8.0, 1 mM EDTA), and extracted in TE buffer containing 2% SDS.
T7523	8120-8187	Sentence	denotes	Protein–DNA cross-links were reverted by heating at 65°C overnight.
T7524	8188-8299	Sentence	denotes	DNA was further purified by QIAquick PCR purification kit (QIAGEN) and eluted in 50 µl sterile distilled water.
T7525	8300-8469	Sentence	denotes	Specific enrichment in NF-κB enhancer sequences was measured by real-time PCR of chromatin immunoprecipitation (ChIP) eluates using SYBR GreenER Master Mix (Invitrogen).
T7526	8470-8598	Sentence	denotes	Reactions were carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) using the following conditions:
T7527	8599-8641	Sentence	denotes	95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T7528	8642-8812	Sentence	denotes	Primers used for MIP-1α, GAPDH and ACTB are listed in Supplementary Data. Real-time PCR of CSF3, LTA, NFKBIA and TLR2 were performed using the Custom ChIP array (QIAGEN).
T7529	8813-8921	Sentence	denotes	For each sample, values were normalized to input DNA and reported as % of input over the rabbit IgG control.
T8155	8923-8943	Sentence	denotes	Statistical analysis
T8156	8944-9017	Sentence	denotes	Statistical analysis was performed by two-tail unpaired Student's t-test.
T8157	9018-9051	Sentence	denotes	Data were reported as means ± SE.
T8158	9052-9155	Sentence	denotes	Differences between the means were considered as statistically significant at the 95% level (P < 0.05).
T36	0-21	Sentence	denotes	MATERIALS AND METHODS
T37	23-31	Sentence	denotes	Plasmids
T38	32-256	Sentence	denotes	The plasmids pcDNA-3xHA-IκB-α, p3xFLAG-CMV-Tat, p3xFLAG-CMV-Tat C(22,25,27)A, p3xFLAG-CMV-Tat R(49,52,53,55,56,57)A, pGEX-2T-Tat, pGEX-2T-Tat C(22,25,27)A and pGEX-2T-Tat R(49,52,53,55,56,57)A were previously described (50).
T39	257-469	Sentence	denotes	The plasmids pNL4-3.Luc.R-E- and pHXB2-env were obtained from the AIDS Research & Reference Reagent Program, Division of AIDS, NIAID, NIH, USA; pκBluc and pSV-β-Gal were purchased from Promega (Madison, WI, USA).
T40	470-735	Sentence	denotes	The plasmids pRc/CMV-3xHA-p65, pRc/CMV-3xHA-p65ΔC(1–318), pRc/CMV-3xHA-p65ΔN(122–551), p3xFLAG-CMV-Tat T,N(23,24)A, p3xFLAG-CMV-Tat K(50,51)A, pGEX-2T-Tat T,N(23,24)A, pGEX-2T-Tat K(50,51)A and pNL4-3.FLAG-Tat.R-E- were generated as described in Supplementary Data.
T41	737-789	Sentence	denotes	Cells, transfection, treatments and luciferase assay
T42	790-1016	Sentence	denotes	HeLa, p50−/−p65−/− mouse embryonic fibroblasts (MEFs) (52) and 293T cells were cultured in Dulbecco's modified Eagle's medium; Jurkat, U937 cells and human peripheral blood mononuclear cells (PBMCs) were cultured in RPMI 1640.
T43	1017-1066	Sentence	denotes	PBMCs were isolated as previously described (53).
T44	1067-1183	Sentence	denotes	Media were supplemented with 10% heat-inactivated fetal calf serum and 2 mM l-glutamine (Lonza Cologne AG, Germany).
T45	1184-1453	Sentence	denotes	HeLa, p50−/−p65−/− MEFs and 293T were transfected with DNA by using FuGENE HD (Roche Diagnostic GmbH, Mannheim, Germany), according to the manufacturer's protocol; total DNA amounts were equalized by transfection of pRc/CMV empty vector (Invitrogen, Carlsbad, CA, USA).
T46	1454-1742	Sentence	denotes	For pulse-stimulation, HeLa cells were treated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St Louis, MO, USA) (20 ng/ml) for 5 min, or tumor necrosis factor-α (TNF-α; Sigma-Aldrich) (20 ng/ml) for 30 min, washed twice in complete culture medium and then returned to culture.
T47	1743-1846	Sentence	denotes	For luciferase assays, pSV-β-Gal was co-transfected with pκBluc to monitor the transfection efficiency.
T48	1847-2146	Sentence	denotes	Forty-eight-hour post-transfection, cells were lysed in lysis buffer of Dual Light Luciferase System (Tropix, Bedford, MA, USA) and the luciferase and β-galactosidase activities were evaluated by using Dual Light Luciferase System (Tropix) in a bioluminometer (Turner Biosystem, Sunnyvale, CA, USA).
T49	2147-2254	Sentence	denotes	The ratio of firefly luciferase activity to β-galactosidase activity was expressed as relative light units.
T50	2256-2272	Sentence	denotes	RNA interference
T51	2273-2398	Sentence	denotes	Jurkat or U937 cells were transfected by electroporation using a Bio-Rad apparatus (Bio-Rad Laboratories, Hercules, CA, USA).
T52	2399-2681	Sentence	denotes	Briefly, aliquots (5 × 106 cells) were suspended in 0.3 ml of RPMI 1640 supplemented with 20% fetal calf serum and subjected to a double electrical pulse (0.22 V, 960 µF) in the presence of annealed siRNA (200 pmol); electroporated cells were washed and cultured in complete medium.
T53	2682-2901	Sentence	denotes	RNA interference was performed with: siRNA Tat sense, CUGCUUGUACCAAUUGCUAUU and siRNA Tat antisense, UAGCAAUUGGUACAAGCAGUU; siRNA control sense, CUGCUUGUCACA AUUGCUAUU and siRNA control antisense, UAGCAAUUGUGACAAGCAGUU.
T54	2902-3016	Sentence	denotes	RNA interference of p65 and IκB-α was performed with SMART pool siRNA p65 and IκB-α (Dharmacon, Chicago, IL, USA).
T55	3018-3064	Sentence	denotes	Pseudotyped virions and single round infection
T56	3065-3247	Sentence	denotes	293T cells (1 × 107) were transfected with pNL4-3.Luc.R-E- or pNL4-3.FLAG-Tat.R-E- (10 µg) together with pHXB2 Env (10 µg), and 48-h post-transfection cell supernatant was collected.
T57	3248-3344	Sentence	denotes	Enzyme-linked immunosorbent assay (ELISA) using anti-p24 antibody measured virion concentration.
T58	3345-3494	Sentence	denotes	PBMCs, Jurkat or U937 cells (5 × 107) were infected with HXB2 Env-pseudotyped virions (500 ng of p24) by spinoculation, as previously described (50).
T59	3496-3563	Sentence	denotes	Cell extracts, western blotting, IKK activity and NF-κB DNA binding
T60	3564-3690	Sentence	denotes	Total, nuclear and cytosolic extracts were performed as previously described (54); details are reported in Supplementary Data.
T61	3691-4239	Sentence	denotes	Western blotting analysis was performed by resuspending protein aliquots in loading buffer (125 mM Tris–HCl, pH 6.8, 5% SDS, 1% bromophenol blue, 10% β-mercaptoethanol, 25% glycerol), resolved on 12% SDS–PAGE, transferred to polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA) and incubated with primary antibodies (1:1000) followed by incubation with horseradish-peroxidase-linked mouse or rabbit IgG (1:2000) (GE Healthcare Amersham, Little Chalfont, Buckinghamshire, UK) in PBS containing 5% non-fat dry milk (Bio-Rad Laboratories).
T62	4240-4330	Sentence	denotes	Proteins were detected by chemiluminescence using the ECL System (GE Healthcare Amersham).
T63	4331-4370	Sentence	denotes	Primary antibodies were purchased from:
T64	4371-4571	Sentence	denotes	Santa Cruz Biotechnology, Santa Cruz, CA, USA (anti-HA F7, anti-IκB-α C15, anti-Histone H1, anti-Hexokinase-II); Sigma-Aldrich (anti-FLAG M2, anti-γ-Tubulin); Upstate, Lake Placid, NY, USA (anti-p65).
T65	4572-4652	Sentence	denotes	Densitometry of single bands was analysed by ImageJ software package (NIH, USA).
T66	4653-4786	Sentence	denotes	IKK activity was evaluated in cytosolic extracts using the HTScan IKK kinase assay kit (Cell Signaling Technology, Danvers, MA, USA).
T67	4787-4973	Sentence	denotes	Binding of p65, p50 and FLAG-Tat to the double-stranded NF-κB oligonucleotide was measured using NF-κB Combo Transcription Factor Assay kit (Cayman Chemical Company, Ann Arbor, MI, USA).
T68	4974-5106	Sentence	denotes	Electrophoretic Mobility Shift Assay (EMSA) was performed as previously described (55); details are described in Supplementary Data.
T69	5108-5128	Sentence	denotes	In vitro translation
T70	5129-5312	Sentence	denotes	HA-IκB-α, p65 and Tat were expressed under the T7 promoter and in vitro translated using the TnT quick coupled transcription/translation system (Promega), as previously reported (50).
T71	5313-5357	Sentence	denotes	Details are described in Supplementary Data.
T72	5359-5402	Sentence	denotes	Immunoprecipitation assay and GST-pull down
T73	5403-5545	Sentence	denotes	Immunoprecipitation, GST-pull down, and production of GST proteins in Escherichia coli strain BL21 were performed as previously reported (50).
T74	5546-5590	Sentence	denotes	Details are described in Supplementary Data.
T75	5592-5605	Sentence	denotes	Real-time PCR
T76	5606-5856	Sentence	denotes	Total RNA was extracted from cells by using the TRIzol reagent (Invitrogen); RNA aliquots (200 ng) were reverse transcribed using Random Examers (Roche) and Superscript III Reverse Transcriptase (Invitrogen), according to the manufacturer's protocol.
T77	5857-6053	Sentence	denotes	Real-time PCR was performed with the iQ Green Super mix (Bio-Rad Laboratories) and carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) under the following conditions:
T78	6054-6096	Sentence	denotes	95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T79	6097-6157	Sentence	denotes	Primers for Tat and MIP-1α are listed in Supplementary Data.
T80	6158-6319	Sentence	denotes	Real-time PCR of CSF3, LTA, NFKBIA, TLR2, GAPDH and ACTB was performed using the RT2 profiler PCR Array-Human NF-κB signaling pathway (QIAGEN Sciences, MD, USA).
T81	6320-6457	Sentence	denotes	Reactions were carried out in triplicate, and gene expression levels were calculated relative to GAPDH mRNA levels as endogenous control.
T82	6458-6538	Sentence	denotes	Relative expression was calculated as 2(Ct gene under investigation − Ct GAPDH).
T83	6540-6575	Sentence	denotes	Chromatin immunoprecipitation assay
T84	6576-6665	Sentence	denotes	Cells were fixed by adding formaldehyde (Sigma-Aldrich) at the final concentration of 1%.
T85	6666-6802	Sentence	denotes	After 10 min, ice-cold PBS plus 0.125 M glycine was added, and plates were transferred on ice, washed extensively with PBS, and scraped.
T86	6803-6981	Sentence	denotes	After centrifugation, cells were 10 min lysed in lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40) supplemented with 1× Complete Protease Inhibitor (Roche Diagnostic GmbH).
T87	6982-7104	Sentence	denotes	Nuclei were pelletted (1000 × g, 5 min), and resuspended in sonication buffer (50 mM Tris–HCl pH 8.0, 1% SDS, 10 mM EDTA).
T88	7105-7369	Sentence	denotes	Chromatin was sonicated using Bandelin Sonoplus GM70 (Bandelin Electronic, Berlin, Germany), centrifuged (14 000 × g, 15 min), and supernatant was 10-fold diluted in dilution buffer (0.01% SDS, 16.7 mM Tris–HCl pH 8.0, 1.1% Triton X-100, 167 mM NaCl, 1.2 mM EDTA).
T89	7370-7520	Sentence	denotes	Samples were pre-cleared by 3-h incubation with 20 µl of protein G agarose beads followed by incubation with antibodies against the analysed proteins.
T90	7521-7672	Sentence	denotes	Primary antibodies were: anti-p65 (sc-372), anti-IκBα (sc-203) and rabbit IgG (sc-2027) from Santa Cruz Biotechnology; anti-FLAG M2 from Sigma–Aldrich.
T91	7673-8119	Sentence	denotes	Immunoprecipitations were carried out at 4°C overnight and immune complexes were collected with protein G agarose beads, washed five times with low salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl), four times with high salt buffer (20 mM Tris–HCl pH 8.0, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 500 mM NaCl), once with TE buffer (10 mM Tris–HCl pH 8.0, 1 mM EDTA), and extracted in TE buffer containing 2% SDS.
T92	8120-8187	Sentence	denotes	Protein–DNA cross-links were reverted by heating at 65°C overnight.
T93	8188-8299	Sentence	denotes	DNA was further purified by QIAquick PCR purification kit (QIAGEN) and eluted in 50 µl sterile distilled water.
T94	8300-8469	Sentence	denotes	Specific enrichment in NF-κB enhancer sequences was measured by real-time PCR of chromatin immunoprecipitation (ChIP) eluates using SYBR GreenER Master Mix (Invitrogen).
T95	8470-8598	Sentence	denotes	Reactions were carried out with the iCycler iQ Real-Time detection system (Bio-Rad Laboratories) using the following conditions:
T96	8599-8641	Sentence	denotes	95°C, 1 min; (94°C, 10 s; 60°C, 30 s) ×40.
T97	8642-8715	Sentence	denotes	Primers used for MIP-1α, GAPDH and ACTB are listed in Supplementary Data.
T98	8716-8812	Sentence	denotes	Real-time PCR of CSF3, LTA, NFKBIA and TLR2 were performed using the Custom ChIP array (QIAGEN).
T99	8813-8921	Sentence	denotes	For each sample, values were normalized to input DNA and reported as % of input over the rabbit IgG control.
T100	8923-8943	Sentence	denotes	Statistical analysis
T101	8944-9017	Sentence	denotes	Statistical analysis was performed by two-tail unpaired Student's t-test.
T102	9018-9051	Sentence	denotes	Data were reported as means ± SE.
T103	9052-9155	Sentence	denotes	Differences between the means were considered as statistically significant at the 95% level (P < 0.05).

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Speculation
T4840	51-61	Protein	denotes	3xHA-IκB-α
T4841	75-78	Protein	denotes	Tat
T4842	92-108	Protein	denotes	Tat C(22,25,27)A
T4843	122-147	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4844	157-160	Protein	denotes	Tat
T4845	170-186	Protein	denotes	Tat C(22,25,27)A
T4846	199-224	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4847	296-299	Protein	denotes	env
T4848	416-421	Protein	denotes	β-Gal
T4849	491-499	Protein	denotes	3xHA-p65
T4850	509-519	Protein	denotes	3xHA-p65ΔC
T4851	536-546	Protein	denotes	3xHA-p65ΔN
T4852	569-584	Protein	denotes	Tat T,N(23,24)A
T4853	598-611	Protein	denotes	Tat K(50,51)A
T4854	621-636	Protein	denotes	Tat T,N(23,24)A
T4855	646-659	Protein	denotes	Tat K(50,51)A
T4856	676-679	Protein	denotes	Tat
T5622	773-783	Protein	denotes	luciferase
T5623	796-799	Protein	denotes	p50
T5624	802-805	Protein	denotes	p65
T5625	1190-1193	Protein	denotes	p50
T5626	1196-1199	Protein	denotes	p65
T5627	1603-1626	Protein	denotes	tumor necrosis factor-α
T5628	1628-1633	Protein	denotes	TNF-α
T5629	1747-1757	Protein	denotes	luciferase
T5630	1770-1775	Protein	denotes	β-Gal
T5631	1930-1940	Protein	denotes	Luciferase
T5632	1983-1993	Protein	denotes	luciferase
T5633	1998-2013	Protein	denotes	β-galactosidase
T8128	7427-7436	Protein	denotes	protein G
T8129	7769-7778	Protein	denotes	protein G
T8130	8659-8665	Protein	denotes	MIP-1α
T8131	8667-8672	Protein	denotes	GAPDH
T8132	8677-8681	Protein	denotes	ACTB
T8133	8733-8737	Protein	denotes	CSF3
T8134	8739-8742	Protein	denotes	LTA
T8135	8744-8750	Protein	denotes	NFKBIA
T8136	8755-8759	Protein	denotes	TLR2
T6014	2719-2734	Protein	denotes	siRNA Tat sense
T6015	2762-2781	Protein	denotes	siRNA Tat antisense
T5634	2168-2178	Protein	denotes	luciferase
T5635	2191-2206	Protein	denotes	β-galactosidase
T6016	2906-2918	Negative_regulation	denotes	interference
T6017	2906-2918	Negative_regulation	denotes	interference
T6018	2922-2925	Protein	denotes	p65
T6019	2930-2935	Protein	denotes	IκB-α
T6020	2966-2975	Protein	denotes	siRNA p65
T6021	2980-2985	Protein	denotes	IκB-α
T6264	3139-3142	Protein	denotes	Tat
T6265	3176-3179	Protein	denotes	Env
T6266	3407-3410	Protein	denotes	Env
T6267	3442-3445	Protein	denotes	p24
T6665	4787-4794	Binding	denotes	Binding	true
T6666	4787-4794	Binding	denotes	Binding	true
T6667	4787-4794	Binding	denotes	Binding	true
T6668	4798-4801	Protein	denotes	p65
T6669	4803-4806	Protein	denotes	p50
T6670	4811-4819	Protein	denotes	FLAG-Tat
T6850	5129-5137	Protein	denotes	HA-IκB-α
T6851	5139-5142	Protein	denotes	p65
T6852	5147-5150	Protein	denotes	Tat
T6853	5156-5165	Gene_expression	denotes	expressed
T6854	5156-5165	Gene_expression	denotes	expressed
T6855	5156-5165	Gene_expression	denotes	expressed
T6856	5166-5171	Positive_regulation	denotes	under
T6857	5166-5171	Positive_regulation	denotes	under
T6858	5166-5171	Positive_regulation	denotes	under
T6971	5389-5392	Protein	denotes	GST
T6972	5424-5427	Protein	denotes	GST
T6973	5457-5460	Protein	denotes	GST
T7461	6109-6112	Protein	denotes	Tat
T7462	6117-6123	Protein	denotes	MIP-1α
T7463	6175-6179	Protein	denotes	CSF3
T7464	6181-6184	Protein	denotes	LTA
T7465	6186-6192	Protein	denotes	NFKBIA
T7466	6194-6198	Protein	denotes	TLR2
T7467	6200-6205	Protein	denotes	GAPDH
T7468	6210-6214	Protein	denotes	ACTB
T7469	6417-6422	Protein	denotes	GAPDH
T7470	6428-6434	Transcription	denotes	levels
T7471	6531-6536	Protein	denotes	GAPDH
R4882	T5628	T5627	equivalentTo	TNF-α,tumor necrosis factor-α
R5176	T6018	T6016	themeOf	p65,interference
R5177	T6019	T6017	themeOf	IκB-α,interference
R5675	T6668	T6667	themeOf	p65,Binding
R5676	T6669	T6666	themeOf	p50,Binding
R5677	T6670	T6665	themeOf	FLAG-Tat,Binding
R5816	T6850	T6853	themeOf	HA-IκB-α,expressed
R5817	T6851	T6855	themeOf	p65,expressed
R5818	T6852	T6854	themeOf	Tat,expressed
R5819	T6853	T6856	themeOf	expressed,under
R5820	T6854	T6858	themeOf	expressed,under
R5821	T6855	T6857	themeOf	expressed,under
R6264	T7469	T7470	themeOf	GAPDH,levels

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T4857	36-61	Protein	denotes	plasmids pcDNA-3xHA-IκB-α
T4858	290-295	Protein	denotes	pHXB2
T4859	296-299	Protein	denotes	env
T4860	401-407	Protein	denotes	pκBluc
T5636	773-783	Protein	denotes	luciferase
T5637	1252-1261	Protein	denotes	FuGENE HD
T5638	1603-1626	Protein	denotes	tumor necrosis factor-α
T5639	1628-1633	Protein	denotes	TNF-α
T5640	1635-1648	Protein	denotes	Sigma-Aldrich
T5641	1747-1757	Protein	denotes	luciferase
T5642	1800-1806	Protein	denotes	pκBluc
T5643	1780-1794	Gene_expression	denotes	co-transfected
T5644	1780-1794	Gene_expression	denotes	co-transfected
T5645	1780-1794	Positive_regulation	denotes	co-transfected
T5646	1780-1794	Positive_regulation	denotes	co-transfected
T5647	1924-1940	Protein	denotes	Light Luciferase
T5648	1983-1993	Protein	denotes	luciferase
T5649	1998-2013	Protein	denotes	β-galactosidase
T5650	2054-2070	Protein	denotes	Light Luciferase
T5651	2168-2178	Protein	denotes	luciferase
T5652	2191-2206	Protein	denotes	β-galactosidase
T6268	3301-3304	Protein	denotes	p24
T6269	3402-3410	Protein	denotes	HXB2 Env
T6270	3442-3445	Protein	denotes	p24
T6671	3529-3532	Protein	denotes	IKK
T6672	3546-3555	Protein	denotes	NF-κB DNA
T6673	3556-3563	Binding	denotes	binding
T6674	4056-4067	Protein	denotes	horseradish
T6675	4068-4078	Protein	denotes	peroxidase
T6676	4435-4444	Protein	denotes	IκB-α C15
T6677	4446-4461	Protein	denotes	anti-Histone H1
T6678	4499-4511	Protein	denotes	anti-FLAG M2
T6679	4513-4527	Protein	denotes	anti-γ-Tubulin
T6680	4653-4656	Protein	denotes	IKK
T6681	4712-4722	Protein	denotes	HTScan IKK
T6682	4798-4801	Protein	denotes	p65
T6683	4803-4806	Protein	denotes	p50
T6684	4811-4815	Protein	denotes	FLAG
T6685	4816-4819	Protein	denotes	Tat
T6686	4843-4848	Protein	denotes	NF-κB
T6687	4884-4889	Protein	denotes	NF-κB
T6688	4849-4864	Entity	denotes	oligonucleotide
T6689	4787-4794	Binding	denotes	Binding
T6690	4787-4794	Binding	denotes	Binding
T6691	4787-4794	Binding	denotes	Binding
T6692	4787-4794	Binding	denotes	Binding
T6859	5129-5131	Protein	denotes	HA
T6860	5132-5137	Protein	denotes	IκB-α
T6861	5139-5142	Protein	denotes	p65
T6862	5147-5150	Protein	denotes	Tat
T6863	5176-5187	Protein	denotes	T7 promoter
T6864	5222-5225	Protein	denotes	TnT
T6865	5156-5165	Gene_expression	denotes	expressed
T6866	5156-5165	Gene_expression	denotes	expressed
T6867	5156-5165	Gene_expression	denotes	expressed
T6868	5156-5165	Gene_expression	denotes	expressed
T6869	5156-5165	Gene_expression	denotes	expressed
T6974	5389-5392	Protein	denotes	GST
T6975	5424-5427	Protein	denotes	GST
T6976	5457-5460	Protein	denotes	GST
T7472	5763-5800	Protein	denotes	Superscript III Reverse Transcriptase
T7473	6109-6112	Protein	denotes	Tat
T7474	6117-6123	Protein	denotes	MIP-1α
T7475	6175-6179	Protein	denotes	CSF3
T7476	6181-6184	Protein	denotes	LTA
T7477	6186-6192	Protein	denotes	NFKBIA
T7478	6194-6198	Protein	denotes	TLR2
T7479	6200-6205	Protein	denotes	GAPDH
T7480	6210-6214	Protein	denotes	ACTB
T7481	6239-6255	Protein	denotes	RT2 profiler PCR
T7482	6268-6273	Protein	denotes	NF-κB
T7483	6417-6427	Protein	denotes	GAPDH mRNA
T7484	6371-6381	Gene_expression	denotes	expression
T7485	6528-6536	Protein	denotes	Ct GAPDH
T7486	6467-6477	Gene_expression	denotes	expression
T8137	6540-6549	Protein	denotes	Chromatin
T8138	6926-6946	Protein	denotes	1× Complete Protease
T8139	6947-6956	Negative_regulation	denotes	Inhibitor
T8140	7105-7114	Protein	denotes	Chromatin
T8141	7551-7554	Protein	denotes	p65
T8142	7565-7583	Protein	denotes	anti-IκBα (sc-203)
T8143	7595-7598	Protein	denotes	IgG
T8144	8323-8337	Protein	denotes	NF-κB enhancer
T8145	8432-8455	Protein	denotes	SYBR GreenER Master Mix
T8146	8659-8665	Protein	denotes	MIP-1α
T8147	8667-8672	Protein	denotes	GAPDH
T8148	8677-8681	Protein	denotes	ACTB
T8149	8733-8737	Protein	denotes	CSF3
T8150	8739-8742	Protein	denotes	LTA
T8151	8744-8750	Protein	denotes	NFKBIA
T8152	8755-8759	Protein	denotes	TLR2
T8153	8909-8912	Protein	denotes	IgG
T6022	2342-2345	Protein	denotes	Rad
T6023	2719-2734	Protein	denotes	siRNA Tat sense
T6024	2736-2757	Protein	denotes	CUGCUUGUACCAAUUGCUAUU
T6025	2762-2771	Protein	denotes	siRNA Tat
T6026	2922-2925	Protein	denotes	p65
T6027	2930-2935	Protein	denotes	IκB-α
T6028	2955-2971	Protein	denotes	SMART pool siRNA
T6029	2972-2975	Protein	denotes	p65
T6030	2980-2985	Protein	denotes	IκB-α
T6031	2906-2918	Negative_regulation	denotes	interference
T6032	2906-2918	Negative_regulation	denotes	interference
R4883	T5641	T5643	themeOf	luciferase,co-transfected
R4884	T5642	T5644	themeOf	pκBluc,co-transfected
R4885	T5643	T5645	themeOf	co-transfected,co-transfected
R4886	T5644	T5646	themeOf	co-transfected,co-transfected
R5178	T6026	T6031	themeOf	p65,interference
R5179	T6027	T6032	themeOf	IκB-α,interference
R5678	T6672	T6673	themeOf	NF-κB DNA,binding
R5679	T6682	T6689	themeOf	p65,Binding
R5680	T6683	T6690	themeOf	p50,Binding
R5681	T6684	T6691	themeOf	FLAG,Binding
R5682	T6685	T6692	themeOf	Tat,Binding
R5683	T6686	T6689	themeOf	NF-κB,Binding
R5684	T6686	T6690	themeOf	NF-κB,Binding
R5685	T6686	T6691	themeOf	NF-κB,Binding
R5686	T6686	T6692	themeOf	NF-κB,Binding
R5687	T6686	T6688	partOf	NF-κB,oligonucleotide
R5688	T6688	T6689	Site	oligonucleotide,Binding
R5689	T6688	T6690	Site	oligonucleotide,Binding
R5690	T6688	T6691	Site	oligonucleotide,Binding
R5691	T6688	T6692	Site	oligonucleotide,Binding
R5822	T6859	T6865	themeOf	HA,expressed
R5823	T6860	T6866	themeOf	IκB-α,expressed
R5824	T6861	T6867	themeOf	p65,expressed
R5825	T6862	T6868	themeOf	Tat,expressed
R5826	T6863	T6869	themeOf	T7 promoter,expressed
R6265	T7483	T7484	themeOf	GAPDH mRNA,expression
R6266	T7485	T7486	themeOf	Ct GAPDH,expression
R6778	T8138	T8139	themeOf	1× Complete Protease,Inhibitor

test2

Id	Subject	Object	Predicate	Lexical cue	Speculation
T4375	51-61	Protein	denotes	3xHA-IκB-α
T4376	75-78	Protein	denotes	Tat
T4377	92-108	Protein	denotes	Tat C(22,25,27)A
T4378	122-147	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4379	157-160	Protein	denotes	Tat
T4380	170-186	Protein	denotes	Tat C(22,25,27)A
T4381	199-224	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4382	296-299	Protein	denotes	env
T4383	416-421	Protein	denotes	β-Gal
T4384	491-499	Protein	denotes	3xHA-p65
T4385	509-519	Protein	denotes	3xHA-p65ΔC
T4386	536-546	Protein	denotes	3xHA-p65ΔN
T4387	569-584	Protein	denotes	Tat T,N(23,24)A
T4388	598-611	Protein	denotes	Tat K(50,51)A
T4389	621-636	Protein	denotes	Tat T,N(23,24)A
T4390	646-659	Protein	denotes	Tat K(50,51)A
T4391	676-679	Protein	denotes	Tat
T4875	773-783	Protein	denotes	luciferase
T4876	796-799	Protein	denotes	p50
T4877	802-805	Protein	denotes	p65
T4878	1190-1193	Protein	denotes	p50
T4879	1196-1199	Protein	denotes	p65
T4880	1603-1626	Protein	denotes	tumor necrosis factor-α
T4881	1628-1633	Protein	denotes	TNF-α
T4882	1747-1757	Protein	denotes	luciferase
T4883	1770-1775	Protein	denotes	β-Gal
T4884	1930-1940	Protein	denotes	Luciferase
T4885	1983-1993	Protein	denotes	luciferase
T4886	1998-2013	Protein	denotes	β-galactosidase
T4887	2168-2178	Protein	denotes	luciferase
T4888	2191-2206	Protein	denotes	β-galactosidase
T4889	2220-2229	Gene_expression	denotes	expressed
T5661	2719-2734	Protein	denotes	siRNA Tat sense
T5662	2762-2781	Protein	denotes	siRNA Tat antisense
T5663	2906-2918	Negative_regulation	denotes	interference
T5664	2922-2925	Protein	denotes	p65
T5665	2930-2935	Protein	denotes	IκB-α
T5666	2966-2975	Protein	denotes	siRNA p65
T5667	2980-2985	Protein	denotes	IκB-α
T6037	3139-3142	Protein	denotes	Tat
T6038	3176-3179	Protein	denotes	Env
T6039	3407-3410	Protein	denotes	Env
T6040	3442-3445	Protein	denotes	p24
T6277	4787-4794	Binding	denotes	Binding	true
T6278	4798-4801	Protein	denotes	p65	true
T6279	4803-4806	Protein	denotes	p50	true
T6280	4811-4819	Protein	denotes	FLAG-Tat
T6702	5129-5137	Protein	denotes	HA-IκB-α
T6703	5139-5142	Protein	denotes	p65
T6704	5147-5150	Protein	denotes	Tat
T6705	5156-5165	Gene_expression	denotes	expressed
T6873	5389-5392	Protein	denotes	GST
T6874	5424-5427	Protein	denotes	GST
T7496	7427-7436	Protein	denotes	protein G
T7497	7769-7778	Protein	denotes	protein G
T7498	8659-8665	Protein	denotes	MIP-1α
T7499	8667-8672	Protein	denotes	GAPDH
T7500	8677-8681	Protein	denotes	ACTB
T7501	8733-8737	Protein	denotes	CSF3
T7502	8739-8742	Protein	denotes	LTA
T7503	8744-8750	Protein	denotes	NFKBIA
T7504	8755-8759	Protein	denotes	TLR2
T6875	5457-5460	Protein	denotes	GST
T6988	6109-6112	Protein	denotes	Tat
T6989	6117-6123	Protein	denotes	MIP-1α
T6990	6175-6179	Protein	denotes	CSF3
T6991	6181-6184	Protein	denotes	LTA
T6992	6186-6192	Protein	denotes	NFKBIA
T6993	6194-6198	Protein	denotes	TLR2
T6994	6200-6205	Protein	denotes	GAPDH
T6995	6210-6214	Protein	denotes	ACTB
T6996	6417-6422	Protein	denotes	GAPDH
T6997	6531-6536	Protein	denotes	GAPDH
R4306	T4881	T4880	equivalentTo	TNF-α,tumor necrosis factor-α
R4889	T5664	T5663	themeOf	p65,interference
R4890	T5665	T5663	themeOf	IκB-α,interference
R5352	T6278	T6277	themeOf	p65,Binding
R5353	T6279	T6277	themeOf	p50,Binding
R5354	T6280	T6277	themeOf	FLAG-Tat,Binding
R5698	T6702	T6705	themeOf	HA-IκB-α,expressed
R5699	T6703	T6705	themeOf	p65,expressed
R5700	T6704	T6705	themeOf	Tat,expressed

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Speculation
T6998	6109-6112	Protein	denotes	Tat
T6999	6117-6123	Protein	denotes	MIP-1α
T7000	6175-6179	Protein	denotes	CSF3
T7001	6181-6184	Protein	denotes	LTA
T7002	6186-6192	Protein	denotes	NFKBIA
T7003	6194-6198	Protein	denotes	TLR2
T7004	6200-6205	Protein	denotes	GAPDH
T7005	6210-6214	Protein	denotes	ACTB
T7006	6417-6422	Protein	denotes	GAPDH
T6041	3139-3142	Protein	denotes	Tat
T6042	3176-3179	Protein	denotes	Env
T6043	3407-3410	Protein	denotes	Env
T6044	3442-3445	Protein	denotes	p24
T6282	4787-4794	Binding	denotes	Binding	true
T6283	4787-4794	Binding	denotes	Binding	true
T6284	4787-4794	Binding	denotes	Binding	true
T6285	4798-4801	Protein	denotes	p65
T6286	4803-4806	Protein	denotes	p50
T6287	4811-4819	Protein	denotes	FLAG-Tat
T7506	7427-7436	Protein	denotes	protein G
T7507	7769-7778	Protein	denotes	protein G
T7508	8659-8665	Protein	denotes	MIP-1α
T7509	8667-8672	Protein	denotes	GAPDH
T7510	8677-8681	Protein	denotes	ACTB
T7511	8733-8737	Protein	denotes	CSF3
T7512	8739-8742	Protein	denotes	LTA
T7513	8744-8750	Protein	denotes	NFKBIA
T7514	8755-8759	Protein	denotes	TLR2
T6876	5389-5392	Protein	denotes	GST
T6877	5424-5427	Protein	denotes	GST
T6878	5457-5460	Protein	denotes	GST
T4892	773-783	Protein	denotes	luciferase
T4893	796-799	Protein	denotes	p50
T4894	802-805	Protein	denotes	p65
T4895	1190-1193	Protein	denotes	p50
T4896	1196-1199	Protein	denotes	p65
T4897	1603-1626	Protein	denotes	tumor necrosis factor-α
T4898	1628-1633	Protein	denotes	TNF-α
T4899	1747-1757	Protein	denotes	luciferase
T4900	1770-1775	Protein	denotes	β-Gal
T4901	1930-1940	Protein	denotes	Luciferase
T4902	1983-1993	Protein	denotes	luciferase
T4903	1998-2013	Protein	denotes	β-galactosidase
T4904	2168-2178	Protein	denotes	luciferase
T4905	2191-2206	Protein	denotes	β-galactosidase
T5669	2719-2734	Protein	denotes	siRNA Tat sense
T5670	2762-2781	Protein	denotes	siRNA Tat antisense
T5671	2906-2918	Negative_regulation	denotes	interference
T5672	2906-2918	Negative_regulation	denotes	interference
T5673	2922-2925	Protein	denotes	p65
T5674	2930-2935	Protein	denotes	IκB-α
T5675	2966-2975	Protein	denotes	siRNA p65
T5676	2980-2985	Protein	denotes	IκB-α
T7007	6428-6434	Transcription	denotes	levels
T7008	6531-6536	Protein	denotes	GAPDH
T4392	51-61	Protein	denotes	3xHA-IκB-α
T4393	75-78	Protein	denotes	Tat
T4394	92-108	Protein	denotes	Tat C(22,25,27)A
T4395	122-147	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4396	157-160	Protein	denotes	Tat
T4397	170-186	Protein	denotes	Tat C(22,25,27)A
T4398	199-224	Protein	denotes	Tat R(49,52,53,55,56,57)A
T4399	296-299	Protein	denotes	env
T4400	416-421	Protein	denotes	β-Gal
T4401	491-499	Protein	denotes	3xHA-p65
T4402	509-519	Protein	denotes	3xHA-p65ΔC
T4403	536-546	Protein	denotes	3xHA-p65ΔN
T4404	569-584	Protein	denotes	Tat T,N(23,24)A
T4405	598-611	Protein	denotes	Tat K(50,51)A
T4406	621-636	Protein	denotes	Tat T,N(23,24)A
T4407	646-659	Protein	denotes	Tat K(50,51)A
T4408	676-679	Protein	denotes	Tat
T6706	5129-5137	Protein	denotes	HA-IκB-α
T6707	5139-5142	Protein	denotes	p65
T6708	5147-5150	Protein	denotes	Tat
T6709	5156-5165	Gene_expression	denotes	expressed
T6710	5156-5165	Gene_expression	denotes	expressed
T6711	5156-5165	Gene_expression	denotes	expressed
T6712	5166-5171	Positive_regulation	denotes	under
T6713	5166-5171	Positive_regulation	denotes	under
T6714	5166-5171	Positive_regulation	denotes	under
R5357	T6287	T6282	themeOf	FLAG-Tat,Binding
R5355	T6285	T6284	themeOf	p65,Binding
R5356	T6286	T6283	themeOf	p50,Binding
R4307	T4898	T4897	equivalentTo	TNF-α,tumor necrosis factor-α
R4891	T5673	T5671	themeOf	p65,interference
R4892	T5674	T5672	themeOf	IκB-α,interference
R5898	T7006	T7007	themeOf	GAPDH,levels
R5701	T6706	T6709	themeOf	HA-IκB-α,expressed
R5702	T6707	T6711	themeOf	p65,expressed
R5703	T6708	T6710	themeOf	Tat,expressed
R5704	T6709	T6712	themeOf	expressed,under
R5705	T6710	T6714	themeOf	expressed,under
R5706	T6711	T6713	themeOf	expressed,under

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T4549	75-78	P04605	denotes	Tat
T4550	75-78	P04608	denotes	Tat
T4551	75-78	P04610	denotes	Tat
T4552	75-78	Q8UMQ1	denotes	Tat
T4553	92-95	P04608	denotes	Tat
T4554	92-95	P04605	denotes	Tat
T4555	92-95	Q8UMQ1	denotes	Tat
T4556	92-95	P04610	denotes	Tat
T4557	122-125	P04608	denotes	Tat
T4558	122-125	P04605	denotes	Tat
T4559	122-125	P04610	denotes	Tat
T4560	122-125	Q8UMQ1	denotes	Tat
T4561	157-160	P04608	denotes	Tat
T4562	157-160	P04605	denotes	Tat
T4563	157-160	Q8UMQ1	denotes	Tat
T4564	157-160	P04610	denotes	Tat
T4565	170-173	P04608	denotes	Tat
T4566	170-173	P04605	denotes	Tat
T4567	170-173	Q8UMQ1	denotes	Tat
T4568	170-173	P04610	denotes	Tat
T4569	199-202	P04610	denotes	Tat
T4570	199-202	P04608	denotes	Tat
T4571	199-202	P04605	denotes	Tat
T4572	199-202	Q8UMQ1	denotes	Tat
T4573	296-299	P04578	denotes	env
T4574	496-499	P21579	denotes	p65
T4575	496-499	Q04206	denotes	p65
T4576	569-572	Q8UMQ1	denotes	Tat
T4577	569-572	P04610	denotes	Tat
T4578	569-572	P04608	denotes	Tat
T4579	569-572	P04605	denotes	Tat
T4580	598-601	Q8UMQ1	denotes	Tat
T4581	598-601	P04610	denotes	Tat
T4582	598-601	P04608	denotes	Tat
T4583	598-601	P04605	denotes	Tat
T4584	621-624	Q8UMQ1	denotes	Tat
T4585	621-624	P04610	denotes	Tat
T4586	621-624	P04608	denotes	Tat
T4587	621-624	P04605	denotes	Tat
T4588	646-649	Q8UMQ1	denotes	Tat
T4589	646-649	P04610	denotes	Tat
T4590	646-649	P04608	denotes	Tat
T4591	646-649	P04605	denotes	Tat
T4592	676-679	Q8UMQ1	denotes	Tat
T4593	676-679	P04610	denotes	Tat
T4594	676-679	P04608	denotes	Tat
T4595	676-679	P04605	denotes	Tat
T5183	773-783	P08659	denotes	luciferase
T5184	796-799	P19838	denotes	p50
T5185	802-805	Q04206	denotes	p65
T5186	802-805	P21579	denotes	p65
T5187	1190-1193	P19838	denotes	p50
T5188	1196-1199	P21579	denotes	p65
T5189	1196-1199	Q04206	denotes	p65
T5190	1603-1624	P01375	denotes	tumor necrosis factor
T5191	1628-1631	P01375	denotes	TNF
T5192	1628-1633	P01375	denotes	TNF-α
T5193	1747-1757	P08659	denotes	luciferase
T5194	1930-1940	P08659	denotes	Luciferase
T5195	1983-1993	P08659	denotes	luciferase
T5196	2060-2070	P08659	denotes	Luciferase
T5197	2168-2178	P08659	denotes	luciferase
T5198	2233-2241	Q04864	denotes	relative
T5812	2725-2728	P04605	denotes	Tat
T5813	2725-2728	P04608	denotes	Tat
T5814	2725-2728	P04610	denotes	Tat
T5815	2725-2728	Q8UMQ1	denotes	Tat
T5816	2768-2771	P04610	denotes	Tat
T5817	2768-2771	P04608	denotes	Tat
T5818	2768-2771	Q8UMQ1	denotes	Tat
T5819	2768-2771	P04605	denotes	Tat
T5820	2922-2925	Q04206	denotes	p65
T5821	2922-2925	P21579	denotes	p65
T5822	2972-2975	Q04206	denotes	p65
T5823	2972-2975	P21579	denotes	p65
T6297	3800-3802	P0A7Z4	denotes	pH
T6298	4566-4569	Q04206	denotes	p65
T6299	4566-4569	P21579	denotes	p65
T6300	4798-4801	Q04206	denotes	p65
T6301	4798-4801	P21579	denotes	p65
T6302	4803-4806	P19838	denotes	p50
T6303	4816-4819	Q8UMQ1	denotes	Tat
T6304	4816-4819	P04610	denotes	Tat
T6305	4816-4819	P04608	denotes	Tat
T6306	4816-4819	P04605	denotes	Tat
T6759	5139-5142	Q04206	denotes	p65
T6760	5139-5142	P21579	denotes	p65
T6761	5147-5150	Q8UMQ1	denotes	Tat
T6762	5147-5150	P04610	denotes	Tat
T6763	5147-5150	P04608	denotes	Tat
T6764	5147-5150	P04605	denotes	Tat
T7186	6109-6112	P04610	denotes	Tat
T7187	6109-6112	Q8UMQ1	denotes	Tat
T7188	6109-6112	P04605	denotes	Tat
T7189	6109-6112	P04608	denotes	Tat
T7190	6117-6123	P10147	denotes	MIP-1α
T7191	6175-6179	P09919	denotes	CSF3
T7192	6181-6184	P01374	denotes	LTA
T7193	6186-6192	P25963	denotes	NFKBIA
T7194	6194-6198	O60603	denotes	TLR2
T7195	6200-6205	P04406	denotes	GAPDH
T7196	6210-6214	P60709	denotes	ACTB
T7197	6405-6413	Q04864	denotes	relative
T7198	6417-6422	P04406	denotes	GAPDH
T7199	6458-6466	Q04864	denotes	Relative
T7200	6531-6536	P04406	denotes	GAPDH
T7530	6877-6879	P0A7Z4	denotes	pH
T7531	7076-7078	P0A7Z4	denotes	pH
T7532	7316-7318	P0A7Z4	denotes	pH
T7533	7551-7554	Q04206	denotes	p65
T7534	7551-7554	P21579	denotes	p65
T7535	7570-7574	P25963	denotes	IκBα
T7536	7849-7851	P0A7Z4	denotes	pH
T7537	7958-7960	P0A7Z4	denotes	pH
T7538	8054-8056	P0A7Z4	denotes	pH
T7539	8659-8665	P10147	denotes	MIP-1α
T7540	8667-8672	P04406	denotes	GAPDH
T7541	8677-8681	P60709	denotes	ACTB
T7542	8733-8737	P09919	denotes	CSF3
T7543	8739-8742	P01374	denotes	LTA
T7544	8744-8750	P25963	denotes	NFKBIA
T7545	8755-8759	O60603	denotes	TLR2
T6125	3139-3142	P04605	denotes	Tat
T6126	3139-3142	P04608	denotes	Tat
T6127	3139-3142	P04610	denotes	Tat
T6128	3139-3142	Q8UMQ1	denotes	Tat
T6129	3176-3179	P04578	denotes	Env
T6130	3301-3304	P03366	denotes	p24
T6131	3301-3304	P04591	denotes	p24
T6132	3301-3304	P04585	denotes	p24
T6133	3301-3304	P12497	denotes	p24
T6134	3407-3410	P04578	denotes	Env
T6135	3442-3445	P04591	denotes	p24
T6136	3442-3445	P04585	denotes	p24
T6137	3442-3445	P12497	denotes	p24
T6138	3442-3445	P03366	denotes	p24

PMC:3333881 / 5825-14980 JSONTXT

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PMC:3333881 / 5825-14980 JSON TXT