PMC:3333881 / 35871-38125 JSON TXT

Annnotations TAB JSON ListView MergeView

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T28825","span":{"begin":609,"end":622},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T28826","span":{"begin":884,"end":887},"obj":"http://purl.obolibrary.org/obo/GO_0008384"},{"id":"T28827","span":{"begin":959,"end":962},"obj":"http://purl.obolibrary.org/obo/GO_0008384"}],"text":"Figure 6. In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65. U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody. Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody. Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H). Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters. Values (mean ± SE, n = 3) are shown. The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P \u003c 0.05)."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T28828","span":{"begin":492,"end":499},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T28829","span":{"begin":775,"end":783},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T28830","span":{"begin":1236,"end":1244},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T28831","span":{"begin":1366,"end":1376},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T28832","span":{"begin":884,"end":887},"obj":"http://purl.obolibrary.org/obo/GO_0008384"},{"id":"T28833","span":{"begin":959,"end":962},"obj":"http://purl.obolibrary.org/obo/GO_0008384"}],"text":"Figure 6. In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65. U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody. Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody. Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H). Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters. Values (mean ± SE, n = 3) are shown. The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P \u003c 0.05)."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T28834","span":{"begin":137,"end":142},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28835","span":{"begin":244,"end":249},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28836","span":{"begin":1014,"end":1019},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28837","span":{"begin":1387,"end":1392},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28838","span":{"begin":1506,"end":1511},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28839","span":{"begin":1708,"end":1713},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28840","span":{"begin":1818,"end":1823},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T28841","span":{"begin":306,"end":313},"obj":"http://purl.obolibrary.org/obo/GO_0019012"},{"id":"T28842","span":{"begin":1086,"end":1093},"obj":"http://purl.obolibrary.org/obo/GO_0019012"},{"id":"T28843","span":{"begin":1568,"end":1575},"obj":"http://purl.obolibrary.org/obo/GO_0019012"},{"id":"T28844","span":{"begin":1880,"end":1887},"obj":"http://purl.obolibrary.org/obo/GO_0019012"},{"id":"T28845","span":{"begin":775,"end":783},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T28846","span":{"begin":1236,"end":1244},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T28847","span":{"begin":1366,"end":1376},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T28848","span":{"begin":775,"end":783},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T28849","span":{"begin":1236,"end":1244},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T28850","span":{"begin":1366,"end":1376},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"Figure 6. In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65. U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody. Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody. Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H). Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters. Values (mean ± SE, n = 3) are shown. The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P \u003c 0.05)."}

sentences

{"project":"sentences","denotations":[{"id":"T28289","span":{"begin":10,"end":131},"obj":"Sentence"},{"id":"T28290","span":{"begin":132,"end":784},"obj":"Sentence"},{"id":"T28291","span":{"begin":785,"end":1114},"obj":"Sentence"},{"id":"T28292","span":{"begin":1115,"end":1245},"obj":"Sentence"},{"id":"T28293","span":{"begin":1246,"end":1596},"obj":"Sentence"},{"id":"T28294","span":{"begin":1597,"end":1908},"obj":"Sentence"},{"id":"T28295","span":{"begin":1909,"end":1996},"obj":"Sentence"},{"id":"T28296","span":{"begin":1997,"end":2078},"obj":"Sentence"},{"id":"T28297","span":{"begin":2079,"end":2115},"obj":"Sentence"},{"id":"T28298","span":{"begin":2116,"end":2254},"obj":"Sentence"},{"id":"T214","span":{"begin":0,"end":9},"obj":"Sentence"},{"id":"T215","span":{"begin":10,"end":131},"obj":"Sentence"},{"id":"T216","span":{"begin":132,"end":784},"obj":"Sentence"},{"id":"T217","span":{"begin":785,"end":1114},"obj":"Sentence"},{"id":"T218","span":{"begin":1115,"end":1245},"obj":"Sentence"},{"id":"T219","span":{"begin":1246,"end":1596},"obj":"Sentence"},{"id":"T220","span":{"begin":1597,"end":1908},"obj":"Sentence"},{"id":"T221","span":{"begin":1909,"end":1996},"obj":"Sentence"},{"id":"T222","span":{"begin":1997,"end":2078},"obj":"Sentence"},{"id":"T223","span":{"begin":2079,"end":2115},"obj":"Sentence"},{"id":"T224","span":{"begin":2116,"end":2254},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 6. In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65. U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody. Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody. Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected. Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H). Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters. Values (mean ± SE, n = 3) are shown. The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P \u003c 0.05)."}

events-check-again

bionlp-st-ge-2016-reference-tees

test2

bionlp-st-ge-2016-reference

bionlp-st-ge-2016-uniprot

PMC:3333881 / 35871-38125 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3333881 / 35871-38125 JSON TXT