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PMC:3333881 / 35871-38125 JSONTXT

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bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T28367 10-12 IN denotes In
T28368 13-18 JJ denotes HIV-1
T28369 19-28 NN denotes infection
T28370 29-32 NNP denotes Tat
T28371 33-41 VBZ denotes sustains
T28372 42-45 DT denotes the
T28373 46-51 JJ denotes NF-κB
T28374 52-60 NN denotes activity
T28375 61-64 CC denotes and
T28376 65-73 VBZ denotes enhances
T28377 74-77 DT denotes the
T28378 78-84 JJ denotes MIP-1α
T28379 85-95 NN denotes expression
T28380 96-99 IN denotes via
T28381 100-111 NN denotes interaction
T28382 112-116 IN denotes with
T28383 117-122 NNP denotes IκB-α
T28384 123-126 CC denotes and
T28385 127-130 NNS denotes p65
T28386 130-131 . denotes .
T28387 132-136 CD denotes U937
T28388 137-142 NNS denotes cells
T28389 143-144 -LRB- denotes (
T28390 144-145 CD denotes 5
T28391 146-147 RB denotes ×
T28392 148-151 CD denotes 107
T28393 151-152 -RRB- denotes )
T28394 153-157 VBD denotes were
T28395 158-169 VBN denotes transfected
T28396 170-174 IN denotes with
T28397 175-180 NNP denotes siRNA
T28398 181-184 NNP denotes Tat
T28399 184-185 , denotes ,
T28400 186-191 NNP denotes siRNA
T28401 192-199 NN denotes control
T28402 200-201 -LRB- denotes (
T28403 201-202 CD denotes 2
T28404 203-207 NN denotes nmol
T28405 207-208 -RRB- denotes )
T28406 208-209 , denotes ,
T28407 210-212 CC denotes or
T28408 213-217 VBD denotes left
T28409 218-231 JJ denotes untransfected
T28410 231-232 : denotes ;
T28411 233-235 CD denotes 24
T28412 236-237 NN denotes h
T28413 238-243 RB denotes later
T28414 244-249 NNS denotes cells
T28415 250-254 VBD denotes were
T28416 255-263 VBN denotes infected
T28417 264-268 IN denotes with
T28418 269-285 JJ denotes HXB2-pseudotyped
T28419 286-291 NN denotes NL4-3
T28420 291-292 . denotes .
T28421 292-300 NN denotes FLAG-Tat
T28422 300-301 . denotes .
T28423 301-302 NN denotes R
T28424 302-303 NN denotes
T28425 303-304 NNP denotes E
T28426 304-305 CD denotes
T28427 306-313 NNS denotes virions
T28428 314-315 -LRB- denotes (
T28429 315-318 CD denotes 500
T28430 319-321 NN denotes ng
T28431 322-324 IN denotes of
T28432 325-328 CD denotes p24
T28433 328-329 -RRB- denotes )
T28434 330-333 CC denotes and
T28435 334-343 VBD denotes harvested
T28436 344-346 IN denotes at
T28437 347-350 DT denotes the
T28438 351-360 JJ denotes indicated
T28439 361-365 NN denotes time
T28440 365-366 . denotes .
T28441 367-368 -LRB- denotes (
T28442 368-369 NNP denotes A
T28443 369-370 -RRB- denotes )
T28444 371-380 JJ denotes Real-time
T28445 381-384 NNP denotes PCR
T28446 385-387 IN denotes of
T28447 388-393 JJ denotes total
T28448 394-397 NNP denotes RNA
T28449 398-406 VBD denotes measured
T28450 407-410 DT denotes the
T28451 411-414 NNP denotes Tat
T28452 415-425 NN denotes expression
T28453 425-426 : denotes ;
T28454 427-432 NNP denotes ELISA
T28455 433-441 VBD denotes measured
T28456 442-445 DT denotes the
T28457 446-452 NN denotes amount
T28458 453-455 IN denotes of
T28459 456-459 CD denotes p24
T28460 460-462 IN denotes in
T28461 463-468 JJ denotes whole
T28462 469-473 NN denotes cell
T28463 474-482 NNS denotes extracts
T28464 482-483 . denotes .
T28465 484-485 -LRB- denotes (
T28466 485-486 NNP denotes B
T28467 486-487 -RRB- denotes )
T28468 488-491 DT denotes The
T28469 492-499 JJ denotes binding
T28470 500-502 IN denotes of
T28471 503-506 CD denotes p65
T28472 507-509 TO denotes to
T28473 510-513 DT denotes the
T28474 514-519 NNP denotes NF-κB
T28475 520-535 JJ denotes double-stranded
T28476 536-551 NN denotes oligonucleotide
T28477 552-555 VBD denotes was
T28478 556-564 VBN denotes measured
T28479 565-567 IN denotes in
T28480 568-575 JJ denotes nuclear
T28481 576-584 NNS denotes extracts
T28482 585-586 -LRB- denotes (
T28483 586-588 CD denotes 10
T28484 589-591 NN denotes µg
T28485 591-592 -RRB- denotes )
T28486 593-598 VBG denotes using
T28487 599-602 DT denotes the
T28488 603-608 NNP denotes NF-κB
T28489 609-622 NNP denotes Transcription
T28490 623-629 NNP denotes Factor
T28491 630-635 NNP denotes ELISA
T28492 636-641 NN denotes assay
T28493 642-645 NN denotes kit
T28494 646-647 -LRB- denotes (
T28495 647-653 NNP denotes Cayman
T28496 653-654 -RRB- denotes )
T28497 654-655 . denotes .
T28498 656-657 -LRB- denotes (
T28499 657-658 $ denotes C
T28500 658-659 -RRB- denotes )
T28501 660-663 DT denotes The
T28502 664-669 JJ denotes IκB-α
T28503 670-677 NN denotes content
T28504 678-681 VBD denotes was
T28505 682-690 VBN denotes analysed
T28506 691-693 IN denotes by
T28507 694-696 CD denotes 12
T28508 696-697 NN denotes %
T28509 698-701 NNP denotes SDS
T28510 702-706 NNP denotes PAGE
T28511 707-710 CC denotes and
T28512 711-718 JJ denotes western
T28513 719-727 VBG denotes blotting
T28514 728-730 IN denotes of
T28515 731-740 JJ denotes cytosolic
T28516 741-749 NNS denotes extracts
T28517 750-751 -LRB- denotes (
T28518 751-753 CD denotes 20
T28519 754-756 NN denotes µg
T28520 756-757 -RRB- denotes )
T28521 758-763 VBG denotes using
T28522 764-774 JJ denotes anti-IκB-α
T28523 775-783 NN denotes antibody
T28524 783-784 . denotes .
T28525 785-797 NNP denotes Densitometry
T28526 798-804 NNS denotes values
T28527 805-806 -LRB- denotes (
T28528 806-807 NNP denotes D
T28529 807-808 -RRB- denotes )
T28530 809-811 IN denotes of
T28531 812-815 DT denotes the
T28532 816-821 NNS denotes bands
T28533 822-826 VBD denotes were
T28534 827-836 VBN denotes expressed
T28535 837-839 IN denotes as
T28536 840-844 VBP denotes fold
T28537 845-853 NN denotes increase
T28538 854-859 IN denotes above
T28539 860-863 DT denotes the
T28540 864-871 NN denotes control
T28541 872-873 -LRB- denotes (
T28542 873-877 JJ denotes mock
T28543 877-878 -RRB- denotes )
T28544 878-879 . denotes .
T28545 880-881 -LRB- denotes (
T28546 881-882 NNP denotes D
T28547 882-883 -RRB- denotes )
T28548 884-887 NNP denotes IKK
T28549 888-896 NN denotes activity
T28550 897-900 VBD denotes was
T28551 901-909 VBN denotes measured
T28552 910-912 IN denotes in
T28553 913-922 JJ denotes cytosolic
T28554 923-927 NN denotes cell
T28555 928-936 NNS denotes extracts
T28556 937-938 -LRB- denotes (
T28557 938-941 CD denotes 100
T28558 942-944 NN denotes µg
T28559 944-945 -RRB- denotes )
T28560 946-951 VBG denotes using
T28561 952-958 NNP denotes HTScan
T28562 959-962 NNP denotes IKK
T28563 963-969 NNP denotes Kinase
T28564 970-975 NNP denotes Assay
T28565 976-977 -LRB- denotes (
T28566 977-981 NNP denotes Cell
T28567 982-991 NNP denotes Signaling
T28568 992-1002 NNP denotes Technology
T28569 1002-1003 -RRB- denotes )
T28570 1003-1004 . denotes .
T28571 1005-1006 -LRB- denotes (
T28572 1006-1007 NNP denotes E
T28573 1007-1008 -RRB- denotes )
T28574 1009-1013 NNP denotes U937
T28575 1014-1019 NNS denotes cells
T28576 1020-1021 -LRB- denotes (
T28577 1021-1022 CD denotes 5
T28578 1023-1024 RB denotes ×
T28579 1025-1028 CD denotes 107
T28580 1028-1029 -RRB- denotes )
T28581 1030-1034 VBD denotes were
T28582 1035-1043 VBN denotes infected
T28583 1044-1048 IN denotes with
T28584 1049-1065 JJ denotes HXB2-pseudotyped
T28585 1066-1071 NN denotes NL4-3
T28586 1071-1072 . denotes .
T28587 1072-1080 NN denotes FLAG-Tat
T28588 1080-1081 . denotes .
T28589 1081-1082 NN denotes R
T28590 1082-1083 NN denotes
T28591 1083-1084 NNP denotes E
T28592 1084-1085 CD denotes
T28593 1086-1093 NNS denotes virions
T28594 1093-1094 , denotes ,
T28595 1095-1097 CC denotes or
T28596 1098-1102 VBD denotes left
T28597 1103-1113 JJ denotes uninfected
T28598 1113-1114 . denotes .
T28599 1115-1131 CD denotes Twenty-four-hour
T28600 1132-1146 NN denotes post-infection
T28601 1146-1147 , denotes ,
T28602 1148-1152 NN denotes cell
T28603 1153-1161 NNS denotes extracts
T28604 1162-1163 -LRB- denotes (
T28605 1163-1164 CD denotes 1
T28606 1165-1167 NN denotes mg
T28607 1167-1168 -RRB- denotes )
T28608 1169-1173 VBD denotes were
T28609 1174-1192 VBN denotes immunoprecipitated
T28610 1193-1197 IN denotes with
T28611 1198-1205 NN denotes protein
T28612 1206-1225 JJ denotes G-Sepharose-coupled
T28613 1226-1235 JJ denotes anti-FLAG
T28614 1236-1244 NN denotes antibody
T28615 1244-1245 . denotes .
T28616 1246-1261 NNS denotes Immunocomplexes
T28617 1262-1266 VBD denotes were
T28618 1267-1276 VBN denotes separated
T28619 1277-1279 IN denotes by
T28620 1280-1282 CD denotes 12
T28621 1282-1283 NN denotes %
T28622 1284-1287 NNP denotes SDS
T28623 1288-1292 NNP denotes PAGE
T28624 1293-1296 CC denotes and
T28625 1297-1305 VBN denotes analysed
T28626 1306-1308 IN denotes by
T28627 1309-1316 JJ denotes western
T28628 1317-1325 VBG denotes blotting
T28629 1326-1330 IN denotes with
T28630 1331-1339 JJ denotes anti-p65
T28631 1339-1340 , denotes ,
T28632 1341-1350 JJ denotes anti-FLAG
T28633 1351-1354 CC denotes and
T28634 1355-1365 JJ denotes anti-IκB-α
T28635 1366-1376 NNS denotes antibodies
T28636 1376-1377 . denotes .
T28637 1378-1379 -LRB- denotes (
T28638 1379-1380 NN denotes F
T28639 1380-1381 -RRB- denotes )
T28640 1382-1386 NNP denotes U937
T28641 1387-1392 NNS denotes cells
T28642 1393-1394 -LRB- denotes (
T28643 1394-1395 CD denotes 5
T28644 1396-1397 RB denotes ×
T28645 1398-1401 CD denotes 107
T28646 1401-1402 -RRB- denotes )
T28647 1403-1407 VBD denotes were
T28648 1408-1419 VBN denotes transfected
T28649 1420-1424 IN denotes with
T28650 1425-1430 NNP denotes siRNA
T28651 1431-1434 NNP denotes Tat
T28652 1434-1435 , denotes ,
T28653 1436-1441 NNP denotes siRNA
T28654 1442-1445 CD denotes p65
T28655 1445-1446 , denotes ,
T28656 1447-1452 NNP denotes siRNA
T28657 1453-1460 NN denotes control
T28658 1461-1462 -LRB- denotes (
T28659 1462-1463 CD denotes 2
T28660 1464-1468 NN denotes nmol
T28661 1468-1469 -RRB- denotes )
T28662 1469-1470 , denotes ,
T28663 1471-1473 CC denotes or
T28664 1474-1478 VBD denotes left
T28665 1479-1492 JJ denotes untransfected
T28666 1492-1493 : denotes ;
T28667 1494-1496 CD denotes 24
T28668 1497-1498 NN denotes h
T28669 1499-1504 RB denotes later
T28670 1504-1505 , denotes ,
T28671 1506-1511 NNS denotes cells
T28672 1512-1516 VBD denotes were
T28673 1517-1525 VBN denotes infected
T28674 1526-1530 IN denotes with
T28675 1531-1547 JJ denotes HXB2-pseudotyped
T28676 1548-1553 NN denotes NL4-3
T28677 1553-1554 . denotes .
T28678 1554-1562 NN denotes FLAG-Tat
T28679 1562-1563 . denotes .
T28680 1563-1564 NN denotes R
T28681 1564-1565 NN denotes
T28682 1565-1566 NNP denotes E
T28683 1566-1567 CD denotes
T28684 1568-1575 NNS denotes virions
T28685 1575-1576 , denotes ,
T28686 1577-1579 CC denotes or
T28687 1580-1584 VBD denotes left
T28688 1585-1595 JJ denotes uninfected
T28689 1595-1596 . denotes .
T28690 1597-1613 CD denotes Twenty-four-hour
T28691 1614-1628 JJ denotes post-infection
T28692 1628-1629 , denotes ,
T28693 1630-1635 JJ denotes total
T28694 1636-1639 NNP denotes RNA
T28695 1640-1643 VBD denotes was
T28696 1644-1652 VBN denotes analysed
T28697 1653-1656 IN denotes for
T28698 1657-1663 JJ denotes MIP-1α
T28699 1664-1674 NN denotes expression
T28700 1675-1677 IN denotes by
T28701 1678-1687 JJ denotes real-time
T28702 1688-1691 NNP denotes PCR
T28703 1691-1692 . denotes .
T28704 1693-1694 -LRB- denotes (
T28705 1694-1695 NNP denotes G
T28706 1696-1699 CC denotes and
T28707 1700-1701 NNP denotes H
T28708 1701-1702 -RRB- denotes )
T28709 1703-1707 NNP denotes U937
T28710 1708-1713 NNS denotes cells
T28711 1714-1715 -LRB- denotes (
T28712 1715-1716 CD denotes 5
T28713 1717-1718 RB denotes ×
T28714 1719-1722 CD denotes 107
T28715 1722-1723 -RRB- denotes )
T28716 1724-1728 VBD denotes were
T28717 1729-1740 VBN denotes transfected
T28718 1741-1745 IN denotes with
T28719 1746-1751 NNP denotes siRNA
T28720 1752-1755 NNP denotes Tat
T28721 1756-1758 CC denotes or
T28722 1759-1764 NNP denotes siRNA
T28723 1765-1772 NN denotes control
T28724 1773-1774 -LRB- denotes (
T28725 1774-1775 CD denotes 2
T28726 1776-1780 NN denotes nmol
T28727 1780-1781 -RRB- denotes )
T28728 1781-1782 , denotes ,
T28729 1783-1785 CC denotes or
T28730 1786-1790 VBD denotes left
T28731 1791-1804 JJ denotes untransfected
T28732 1804-1805 : denotes ;
T28733 1806-1808 CD denotes 24
T28734 1809-1810 NN denotes h
T28735 1811-1816 RB denotes later
T28736 1816-1817 , denotes ,
T28737 1818-1823 NNS denotes cells
T28738 1824-1828 VBD denotes were
T28739 1829-1837 VBN denotes infected
T28740 1838-1842 IN denotes with
T28741 1843-1859 JJ denotes HXB2-pseudotyped
T28742 1860-1865 NN denotes NL4-3
T28743 1865-1866 . denotes .
T28744 1866-1874 NN denotes FLAG-Tat
T28745 1874-1875 . denotes .
T28746 1875-1876 NN denotes R
T28747 1876-1877 NN denotes
T28748 1877-1878 NNP denotes E
T28749 1878-1879 CD denotes
T28750 1880-1887 NNS denotes virions
T28751 1887-1888 , denotes ,
T28752 1889-1891 CC denotes or
T28753 1892-1896 VBD denotes left
T28754 1897-1907 JJ denotes uninfected
T28755 1907-1908 . denotes .
T28756 1909-1925 CD denotes Twenty-four-hour
T28757 1926-1940 NN denotes post-infection
T28758 1940-1941 , denotes ,
T28759 1942-1946 NNP denotes ChIP
T28760 1947-1950 VBD denotes was
T28761 1951-1960 VBN denotes performed
T28762 1961-1965 IN denotes with
T28763 1966-1974 JJ denotes anti-p65
T28764 1975-1976 -LRB- denotes (
T28765 1976-1977 NNP denotes G
T28766 1977-1978 -RRB- denotes )
T28767 1979-1981 CC denotes or
T28768 1982-1991 JJ denotes anti-FLAG
T28769 1992-1993 -LRB- denotes (
T28770 1993-1994 NNP denotes H
T28771 1994-1995 -RRB- denotes )
T28772 1995-1996 . denotes .
T28773 1997-2006 JJ denotes Real-time
T28774 2007-2010 NNP denotes PCR
T28775 2011-2014 VBD denotes was
T28776 2015-2024 VBN denotes performed
T28777 2025-2029 IN denotes with
T28778 2030-2037 NNS denotes primers
T28779 2038-2046 JJ denotes specific
T28780 2047-2050 IN denotes for
T28781 2051-2057 NNP denotes MIP-1α
T28782 2058-2061 CC denotes and
T28783 2062-2067 NNP denotes GAPDH
T28784 2068-2077 NNS denotes promoters
T28785 2077-2078 . denotes .
T28786 2079-2085 NNS denotes Values
T28787 2086-2087 -LRB- denotes (
T28788 2087-2091 JJ denotes mean
T28789 2092-2093 NN denotes ±
T28790 2094-2096 NNP denotes SE
T28791 2096-2097 , denotes ,
T28792 2098-2099 JJ denotes n
T28793 2100-2101 SYM denotes =
T28794 2102-2103 LS denotes 3
T28795 2103-2104 -RRB- denotes )
T28796 2105-2108 VBP denotes are
T28797 2109-2114 VBN denotes shown
T28798 2114-2115 . denotes .
T28799 2116-2119 DT denotes The
T28800 2120-2129 NNS denotes asterisks
T28801 2130-2138 VBP denotes indicate
T28802 2139-2152 RB denotes statistically
T28803 2153-2164 JJ denotes significant
T28804 2165-2176 NNS denotes differences
T28805 2177-2185 VBN denotes compared
T28806 2186-2188 TO denotes to
T28807 2189-2192 DT denotes the
T28808 2193-2200 NN denotes control
T28809 2201-2202 -LRB- denotes (
T28810 2202-2206 JJ denotes mock
T28811 2206-2207 -RRB- denotes )
T28812 2207-2208 , denotes ,
T28813 2209-2218 VBG denotes according
T28814 2219-2221 TO denotes to
T28815 2222-2225 DT denotes the
T28816 2226-2233 NN denotes Student
T28817 2233-2235 POS denotes 's
T28818 2236-2242 JJS denotes t-test
T28819 2243-2244 -LRB- denotes (
T28820 2244-2245 NNP denotes P
T28821 2246-2247 CD denotes <
T28822 2248-2252 CD denotes 0.05
T28823 2252-2253 -RRB- denotes )
T28824 2253-2254 . denotes .
R22137 T28367 T28371 prep In,sustains
R22138 T28368 T28369 compound HIV-1,infection
R22139 T28369 T28367 pobj infection,In
R22140 T28370 T28371 nsubj Tat,sustains
R22141 T28371 T28371 ROOT sustains,sustains
R22142 T28372 T28374 det the,activity
R22143 T28373 T28374 amod NF-κB,activity
R22144 T28374 T28371 dobj activity,sustains
R22145 T28375 T28371 cc and,sustains
R22146 T28376 T28371 conj enhances,sustains
R22147 T28377 T28379 det the,expression
R22148 T28378 T28379 amod MIP-1α,expression
R22149 T28379 T28376 dobj expression,enhances
R22150 T28380 T28376 prep via,enhances
R22151 T28381 T28380 pobj interaction,via
R22152 T28382 T28381 prep with,interaction
R22153 T28383 T28382 pobj IκB-α,with
R22154 T28384 T28383 cc and,IκB-α
R22155 T28385 T28383 conj p65,IκB-α
R22156 T28386 T28371 punct .,sustains
R22157 T28387 T28388 nummod U937,cells
R22158 T28388 T28395 nsubjpass cells,transfected
R22159 T28389 T28388 punct (,cells
R22160 T28390 T28392 quantmod 5,107
R22161 T28391 T28392 quantmod ×,107
R22162 T28392 T28388 appos 107,cells
R22163 T28393 T28388 punct ),cells
R22164 T28394 T28395 auxpass were,transfected
R22165 T28395 T28416 ccomp transfected,infected
R22166 T28396 T28395 prep with,transfected
R22167 T28397 T28398 compound siRNA,Tat
R22168 T28398 T28396 pobj Tat,with
R22169 T28399 T28395 punct ",",transfected
R22170 T28400 T28401 compound siRNA,control
R22171 T28401 T28395 appos control,transfected
R22172 T28402 T28404 punct (,nmol
R22173 T28403 T28404 nummod 2,nmol
R22174 T28404 T28401 appos nmol,control
R22175 T28405 T28401 punct ),control
R22176 T28406 T28401 punct ",",control
R22177 T28407 T28395 cc or,transfected
R22178 T28408 T28395 conj left,transfected
R22179 T28409 T28408 oprd untransfected,left
R22180 T28410 T28416 punct ;,infected
R22181 T28411 T28412 nummod 24,h
R22182 T28412 T28416 npadvmod h,infected
R22183 T28413 T28414 amod later,cells
R22184 T28414 T28416 nsubjpass cells,infected
R22185 T28415 T28416 auxpass were,infected
R22186 T28416 T28416 ROOT infected,infected
R22187 T28417 T28416 prep with,infected
R22188 T28418 T28419 amod HXB2-pseudotyped,NL4-3
R22189 T28419 T28417 pobj NL4-3,with
R22190 T28420 T28416 punct .,infected
R22191 T28421 T28421 ROOT FLAG-Tat,FLAG-Tat
R22192 T28422 T28421 punct .,FLAG-Tat
R22193 T28423 T28423 ROOT R,R
R22194 T28424 T28435 nsubj −,harvested
R22195 T28425 T28426 compound E,−
R22196 T28426 T28427 nummod −,virions
R22197 T28427 T28424 appos virions,−
R22198 T28428 T28430 punct (,ng
R22199 T28429 T28430 nummod 500,ng
R22200 T28430 T28427 parataxis ng,virions
R22201 T28431 T28430 prep of,ng
R22202 T28432 T28431 pobj p24,of
R22203 T28433 T28430 punct ),ng
R22204 T28434 T28424 cc and,−
R22205 T28435 T28423 acl harvested,R
R22206 T28436 T28435 prep at,harvested
R22207 T28437 T28439 det the,time
R22208 T28438 T28439 amod indicated,time
R22209 T28439 T28436 pobj time,at
R22210 T28440 T28423 punct .,R
R22211 T28441 T28442 punct (,A
R22212 T28442 T28442 ROOT A,A
R22213 T28443 T28442 punct ),A
R22214 T28444 T28445 amod Real-time,PCR
R22215 T28445 T28449 nsubj PCR,measured
R22216 T28446 T28445 prep of,PCR
R22217 T28447 T28448 amod total,RNA
R22218 T28448 T28446 pobj RNA,of
R22219 T28449 T28455 ccomp measured,measured
R22220 T28450 T28452 det the,expression
R22221 T28451 T28452 compound Tat,expression
R22222 T28452 T28449 dobj expression,measured
R22223 T28453 T28455 punct ;,measured
R22224 T28454 T28455 nsubj ELISA,measured
R22225 T28455 T28455 ROOT measured,measured
R22226 T28456 T28457 det the,amount
R22227 T28457 T28455 dobj amount,measured
R22228 T28458 T28457 prep of,amount
R22229 T28459 T28458 pobj p24,of
R22230 T28460 T28459 prep in,p24
R22231 T28461 T28463 amod whole,extracts
R22232 T28462 T28463 compound cell,extracts
R22233 T28463 T28460 pobj extracts,in
R22234 T28464 T28455 punct .,measured
R22235 T28465 T28466 punct (,B
R22236 T28466 T28466 ROOT B,B
R22237 T28467 T28466 punct ),B
R22238 T28468 T28469 det The,binding
R22239 T28469 T28478 nsubjpass binding,measured
R22240 T28470 T28469 prep of,binding
R22241 T28471 T28470 pobj p65,of
R22242 T28472 T28469 prep to,binding
R22243 T28473 T28476 det the,oligonucleotide
R22244 T28474 T28476 nmod NF-κB,oligonucleotide
R22245 T28475 T28476 amod double-stranded,oligonucleotide
R22246 T28476 T28472 pobj oligonucleotide,to
R22247 T28477 T28478 auxpass was,measured
R22248 T28478 T28478 ROOT measured,measured
R22249 T28479 T28478 prep in,measured
R22250 T28480 T28481 amod nuclear,extracts
R22251 T28481 T28479 pobj extracts,in
R22252 T28482 T28481 punct (,extracts
R22253 T28483 T28484 nummod 10,µg
R22254 T28484 T28481 appos µg,extracts
R22255 T28485 T28481 punct ),extracts
R22256 T28486 T28478 advcl using,measured
R22257 T28487 T28493 det the,kit
R22258 T28488 T28491 compound NF-κB,ELISA
R22259 T28489 T28491 compound Transcription,ELISA
R22260 T28490 T28491 compound Factor,ELISA
R22261 T28491 T28493 compound ELISA,kit
R22262 T28492 T28493 compound assay,kit
R22263 T28493 T28486 dobj kit,using
R22264 T28494 T28493 punct (,kit
R22265 T28495 T28493 appos Cayman,kit
R22266 T28496 T28493 punct ),kit
R22267 T28497 T28478 punct .,measured
R22268 T28498 T28499 punct (,C
R22269 T28499 T28499 ROOT C,C
R22270 T28500 T28499 punct ),C
R22271 T28501 T28503 det The,content
R22272 T28502 T28503 amod IκB-α,content
R22273 T28503 T28505 nsubjpass content,analysed
R22274 T28504 T28505 auxpass was,analysed
R22275 T28505 T28505 ROOT analysed,analysed
R22276 T28506 T28505 prep by,analysed
R22277 T28507 T28508 nummod 12,%
R22278 T28508 T28509 compound %,SDS
R22279 T28509 T28506 pobj SDS,by
R22280 T28510 T28509 conj PAGE,SDS
R22281 T28511 T28510 cc and,PAGE
R22282 T28512 T28513 amod western,blotting
R22283 T28513 T28510 conj blotting,PAGE
R22284 T28514 T28513 prep of,blotting
R22285 T28515 T28516 amod cytosolic,extracts
R22286 T28516 T28514 pobj extracts,of
R22287 T28517 T28516 punct (,extracts
R22288 T28518 T28519 nummod 20,µg
R22289 T28519 T28516 appos µg,extracts
R22290 T28520 T28516 punct ),extracts
R22291 T28521 T28505 advcl using,analysed
R22292 T28522 T28523 amod anti-IκB-α,antibody
R22293 T28523 T28521 dobj antibody,using
R22294 T28524 T28505 punct .,analysed
R22295 T28525 T28526 compound Densitometry,values
R22296 T28526 T28534 nsubjpass values,expressed
R22297 T28527 T28528 punct (,D
R22298 T28528 T28526 appos D,values
R22299 T28529 T28528 punct ),D
R22300 T28530 T28528 prep of,D
R22301 T28531 T28532 det the,bands
R22302 T28532 T28530 pobj bands,of
R22303 T28533 T28534 auxpass were,expressed
R22304 T28534 T28534 ROOT expressed,expressed
R22305 T28535 T28536 mark as,fold
R22306 T28536 T28534 advcl fold,expressed
R22307 T28537 T28536 dobj increase,fold
R22308 T28538 T28536 prep above,fold
R22309 T28539 T28540 det the,control
R22310 T28540 T28538 pobj control,above
R22311 T28541 T28540 punct (,control
R22312 T28542 T28540 amod mock,control
R22313 T28543 T28540 punct ),control
R22314 T28544 T28534 punct .,expressed
R22315 T28545 T28551 punct (,measured
R22316 T28546 T28549 compound D,activity
R22317 T28547 T28548 punct ),IKK
R22318 T28548 T28549 compound IKK,activity
R22319 T28549 T28551 nsubjpass activity,measured
R22320 T28550 T28551 auxpass was,measured
R22321 T28551 T28551 ROOT measured,measured
R22322 T28552 T28551 prep in,measured
R22323 T28553 T28554 amod cytosolic,cell
R22324 T28554 T28555 compound cell,extracts
R22325 T28555 T28552 pobj extracts,in
R22326 T28556 T28555 punct (,extracts
R22327 T28557 T28558 nummod 100,µg
R22328 T28558 T28555 appos µg,extracts
R22329 T28559 T28555 punct ),extracts
R22330 T28560 T28551 advcl using,measured
R22331 T28561 T28564 compound HTScan,Assay
R22332 T28562 T28564 compound IKK,Assay
R22333 T28563 T28564 compound Kinase,Assay
R22334 T28564 T28560 dobj Assay,using
R22335 T28565 T28568 punct (,Technology
R22336 T28566 T28568 compound Cell,Technology
R22337 T28567 T28568 compound Signaling,Technology
R22338 T28568 T28564 appos Technology,Assay
R22339 T28569 T28564 punct ),Assay
R22340 T28570 T28551 punct .,measured
R22341 T28571 T28582 punct (,infected
R22342 T28572 T28575 nmod E,cells
R22343 T28573 T28572 punct ),E
R22344 T28574 T28575 compound U937,cells
R22345 T28575 T28582 nsubjpass cells,infected
R22346 T28576 T28575 punct (,cells
R22347 T28577 T28579 nummod 5,107
R22348 T28578 T28579 quantmod ×,107
R22349 T28579 T28575 appos 107,cells
R22350 T28580 T28575 punct ),cells
R22351 T28581 T28582 auxpass were,infected
R22352 T28582 T28582 ROOT infected,infected
R22353 T28583 T28582 prep with,infected
R22354 T28584 T28585 amod HXB2-pseudotyped,NL4-3
R22355 T28585 T28583 pobj NL4-3,with
R22356 T28586 T28582 punct .,infected
R22357 T28587 T28587 ROOT FLAG-Tat,FLAG-Tat
R22358 T28588 T28587 punct .,FLAG-Tat
R22359 T28589 T28593 nmod R,virions
R22360 T28590 T28593 nummod −,virions
R22361 T28591 T28592 compound E,−
R22362 T28592 T28593 nummod −,virions
R22363 T28593 T28596 nsubj virions,left
R22364 T28594 T28593 punct ",",virions
R22365 T28595 T28593 cc or,virions
R22366 T28596 T28596 ROOT left,left
R22367 T28597 T28596 oprd uninfected,left
R22368 T28598 T28596 punct .,left
R22369 T28599 T28600 nummod Twenty-four-hour,post-infection
R22370 T28600 T28609 nsubjpass post-infection,immunoprecipitated
R22371 T28601 T28603 punct ",",extracts
R22372 T28602 T28603 compound cell,extracts
R22373 T28603 T28600 appos extracts,post-infection
R22374 T28604 T28603 punct (,extracts
R22375 T28605 T28606 nummod 1,mg
R22376 T28606 T28603 appos mg,extracts
R22377 T28607 T28603 punct ),extracts
R22378 T28608 T28609 auxpass were,immunoprecipitated
R22379 T28609 T28609 ROOT immunoprecipitated,immunoprecipitated
R22380 T28610 T28609 prep with,immunoprecipitated
R22381 T28611 T28614 nmod protein,antibody
R22382 T28612 T28614 amod G-Sepharose-coupled,antibody
R22383 T28613 T28614 amod anti-FLAG,antibody
R22384 T28614 T28610 pobj antibody,with
R22385 T28615 T28609 punct .,immunoprecipitated
R22386 T28616 T28618 nsubjpass Immunocomplexes,separated
R22387 T28617 T28618 auxpass were,separated
R22388 T28618 T28618 ROOT separated,separated
R22389 T28619 T28618 prep by,separated
R22390 T28620 T28621 nummod 12,%
R22391 T28621 T28619 pobj %,by
R22392 T28622 T28623 compound SDS,PAGE
R22393 T28623 T28618 npadvmod PAGE,separated
R22394 T28624 T28623 cc and,PAGE
R22395 T28625 T28623 conj analysed,PAGE
R22396 T28626 T28625 agent by,analysed
R22397 T28627 T28628 amod western,blotting
R22398 T28628 T28626 pobj blotting,by
R22399 T28629 T28628 prep with,blotting
R22400 T28630 T28635 amod anti-p65,antibodies
R22401 T28631 T28630 punct ",",anti-p65
R22402 T28632 T28630 conj anti-FLAG,anti-p65
R22403 T28633 T28632 cc and,anti-FLAG
R22404 T28634 T28632 conj anti-IκB-α,anti-FLAG
R22405 T28635 T28629 pobj antibodies,with
R22406 T28636 T28618 punct .,separated
R22407 T28637 T28648 punct (,transfected
R22408 T28638 T28641 compound F,cells
R22409 T28639 T28640 punct ),U937
R22410 T28640 T28641 compound U937,cells
R22411 T28641 T28648 nsubjpass cells,transfected
R22412 T28642 T28641 punct (,cells
R22413 T28643 T28645 quantmod 5,107
R22414 T28644 T28645 quantmod ×,107
R22415 T28645 T28641 appos 107,cells
R22416 T28646 T28641 punct ),cells
R22417 T28647 T28648 auxpass were,transfected
R22418 T28648 T28673 ccomp transfected,infected
R22419 T28649 T28648 prep with,transfected
R22420 T28650 T28651 compound siRNA,Tat
R22421 T28651 T28649 pobj Tat,with
R22422 T28652 T28648 punct ",",transfected
R22423 T28653 T28648 dep siRNA,transfected
R22424 T28654 T28653 appos p65,siRNA
R22425 T28655 T28653 punct ",",siRNA
R22426 T28656 T28657 compound siRNA,control
R22427 T28657 T28653 appos control,siRNA
R22428 T28658 T28660 punct (,nmol
R22429 T28659 T28660 nummod 2,nmol
R22430 T28660 T28657 appos nmol,control
R22431 T28661 T28657 punct ),control
R22432 T28662 T28653 punct ",",siRNA
R22433 T28663 T28648 cc or,transfected
R22434 T28664 T28648 conj left,transfected
R22435 T28665 T28664 oprd untransfected,left
R22436 T28666 T28673 punct ;,infected
R22437 T28667 T28668 nummod 24,h
R22438 T28668 T28669 npadvmod h,later
R22439 T28669 T28673 advmod later,infected
R22440 T28670 T28673 punct ",",infected
R22441 T28671 T28673 nsubjpass cells,infected
R22442 T28672 T28673 auxpass were,infected
R22443 T28673 T28673 ROOT infected,infected
R22444 T28674 T28673 prep with,infected
R22445 T28675 T28676 amod HXB2-pseudotyped,NL4-3
R22446 T28676 T28674 pobj NL4-3,with
R22447 T28677 T28673 punct .,infected
R22448 T28678 T28678 ROOT FLAG-Tat,FLAG-Tat
R22449 T28679 T28678 punct .,FLAG-Tat
R22450 T28680 T28684 nmod R,virions
R22451 T28681 T28684 nummod −,virions
R22452 T28682 T28683 compound E,−
R22453 T28683 T28684 nummod −,virions
R22454 T28684 T28687 nsubj virions,left
R22455 T28685 T28684 punct ",",virions
R22456 T28686 T28684 cc or,virions
R22457 T28687 T28687 ROOT left,left
R22458 T28688 T28687 oprd uninfected,left
R22459 T28689 T28687 punct .,left
R22460 T28690 T28691 nummod Twenty-four-hour,post-infection
R22461 T28691 T28695 nsubj post-infection,was
R22462 T28692 T28691 punct ",",post-infection
R22463 T28693 T28694 amod total,RNA
R22464 T28694 T28691 appos RNA,post-infection
R22465 T28695 T28695 ROOT was,was
R22466 T28696 T28695 attr analysed,was
R22467 T28697 T28696 prep for,analysed
R22468 T28698 T28699 amod MIP-1α,expression
R22469 T28699 T28697 pobj expression,for
R22470 T28700 T28699 prep by,expression
R22471 T28701 T28702 amod real-time,PCR
R22472 T28702 T28700 pobj PCR,by
R22473 T28703 T28695 punct .,was
R22474 T28704 T28709 punct (,U937
R22475 T28705 T28709 nmod G,U937
R22476 T28706 T28705 cc and,G
R22477 T28707 T28705 conj H,G
R22478 T28708 T28709 punct ),U937
R22479 T28709 T28710 compound U937,cells
R22480 T28710 T28717 nsubjpass cells,transfected
R22481 T28711 T28710 punct (,cells
R22482 T28712 T28714 quantmod 5,107
R22483 T28713 T28714 quantmod ×,107
R22484 T28714 T28710 appos 107,cells
R22485 T28715 T28710 punct ),cells
R22486 T28716 T28717 auxpass were,transfected
R22487 T28717 T28739 ccomp transfected,infected
R22488 T28718 T28717 prep with,transfected
R22489 T28719 T28720 compound siRNA,Tat
R22490 T28720 T28718 pobj Tat,with
R22491 T28721 T28720 cc or,Tat
R22492 T28722 T28723 compound siRNA,control
R22493 T28723 T28720 conj control,Tat
R22494 T28724 T28726 punct (,nmol
R22495 T28725 T28726 nummod 2,nmol
R22496 T28726 T28723 appos nmol,control
R22497 T28727 T28726 punct ),nmol
R22498 T28728 T28723 punct ",",control
R22499 T28729 T28723 cc or,control
R22500 T28730 T28717 advcl left,transfected
R22501 T28731 T28730 oprd untransfected,left
R22502 T28732 T28739 punct ;,infected
R22503 T28733 T28734 nummod 24,h
R22504 T28734 T28735 npadvmod h,later
R22505 T28735 T28739 advmod later,infected
R22506 T28736 T28739 punct ",",infected
R22507 T28737 T28739 nsubjpass cells,infected
R22508 T28738 T28739 auxpass were,infected
R22509 T28739 T28739 ROOT infected,infected
R22510 T28740 T28739 prep with,infected
R22511 T28741 T28742 amod HXB2-pseudotyped,NL4-3
R22512 T28742 T28740 pobj NL4-3,with
R22513 T28743 T28739 punct .,infected
R22514 T28744 T28744 ROOT FLAG-Tat,FLAG-Tat
R22515 T28745 T28744 punct .,FLAG-Tat
R22516 T28746 T28750 nmod R,virions
R22517 T28747 T28750 nummod −,virions
R22518 T28748 T28749 compound E,−
R22519 T28749 T28750 nummod −,virions
R22520 T28750 T28753 nsubj virions,left
R22521 T28751 T28750 punct ",",virions
R22522 T28752 T28750 cc or,virions
R22523 T28753 T28753 ROOT left,left
R22524 T28754 T28753 oprd uninfected,left
R22525 T28755 T28753 punct .,left
R22526 T28756 T28757 nummod Twenty-four-hour,post-infection
R22527 T28757 T28761 npadvmod post-infection,performed
R22528 T28758 T28761 punct ",",performed
R22529 T28759 T28761 nsubjpass ChIP,performed
R22530 T28760 T28761 auxpass was,performed
R22531 T28761 T28761 ROOT performed,performed
R22532 T28762 T28761 prep with,performed
R22533 T28763 T28762 pobj anti-p65,with
R22534 T28764 T28765 punct (,G
R22535 T28765 T28763 appos G,anti-p65
R22536 T28766 T28761 punct ),performed
R22537 T28767 T28761 cc or,performed
R22538 T28768 T28761 conj anti-FLAG,performed
R22539 T28769 T28770 punct (,H
R22540 T28770 T28768 appos H,anti-FLAG
R22541 T28771 T28768 punct ),anti-FLAG
R22542 T28772 T28761 punct .,performed
R22543 T28773 T28774 compound Real-time,PCR
R22544 T28774 T28776 nsubjpass PCR,performed
R22545 T28775 T28776 auxpass was,performed
R22546 T28776 T28776 ROOT performed,performed
R22547 T28777 T28776 prep with,performed
R22548 T28778 T28777 pobj primers,with
R22549 T28779 T28778 amod specific,primers
R22550 T28780 T28779 prep for,specific
R22551 T28781 T28780 pobj MIP-1α,for
R22552 T28782 T28781 cc and,MIP-1α
R22553 T28783 T28781 conj GAPDH,MIP-1α
R22554 T28784 T28778 conj promoters,primers
R22555 T28785 T28776 punct .,performed
R22556 T28786 T28797 nsubjpass Values,shown
R22557 T28787 T28790 punct (,SE
R22558 T28788 T28790 amod mean,SE
R22559 T28789 T28790 compound ±,SE
R22560 T28790 T28786 appos SE,Values
R22561 T28791 T28790 punct ",",SE
R22562 T28792 T28797 nsubjpass n,shown
R22563 T28793 T28794 punct =,3
R22564 T28794 T28792 advmod 3,n
R22565 T28795 T28797 punct ),shown
R22566 T28796 T28797 auxpass are,shown
R22567 T28797 T28797 ROOT shown,shown
R22568 T28798 T28797 punct .,shown
R22569 T28799 T28800 det The,asterisks
R22570 T28800 T28801 nsubj asterisks,indicate
R22571 T28801 T28801 ROOT indicate,indicate
R22572 T28802 T28803 advmod statistically,significant
R22573 T28803 T28804 amod significant,differences
R22574 T28804 T28801 dobj differences,indicate
R22575 T28805 T28804 acl compared,differences
R22576 T28806 T28805 prep to,compared
R22577 T28807 T28808 det the,control
R22578 T28808 T28806 pobj control,to
R22579 T28809 T28808 punct (,control
R22580 T28810 T28808 amod mock,control
R22581 T28811 T28808 punct ),control
R22582 T28812 T28801 punct ",",indicate
R22583 T28813 T28801 prep according,indicate
R22584 T28814 T28813 prep to,according
R22585 T28815 T28816 det the,Student
R22586 T28816 T28818 poss Student,t-test
R22587 T28817 T28816 case 's,Student
R22588 T28818 T28814 pobj t-test,to
R22589 T28819 T28818 punct (,t-test
R22590 T28820 T28823 nmod P,)
R22591 T28821 T28820 nummod <,P
R22592 T28822 T28820 nummod 0.05,P
R22593 T28823 T28818 punct ),t-test
R22594 T28824 T28801 punct .,indicate

GO-BP

Id Subject Object Predicate Lexical cue
T28825 609-622 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T28826 884-887 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T28827 959-962 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK

GO-MF

Id Subject Object Predicate Lexical cue
T28828 492-499 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T28829 775-783 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T28830 1236-1244 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T28831 1366-1376 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T28832 884-887 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T28833 959-962 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK

GO-CC

Id Subject Object Predicate Lexical cue
T28834 137-142 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28835 244-249 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28836 1014-1019 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28837 1387-1392 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28838 1506-1511 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28839 1708-1713 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28840 1818-1823 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T28841 306-313 http://purl.obolibrary.org/obo/GO_0019012 denotes virions
T28842 1086-1093 http://purl.obolibrary.org/obo/GO_0019012 denotes virions
T28843 1568-1575 http://purl.obolibrary.org/obo/GO_0019012 denotes virions
T28844 1880-1887 http://purl.obolibrary.org/obo/GO_0019012 denotes virions
T28845 775-783 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T28846 1236-1244 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T28847 1366-1376 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T28848 775-783 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T28849 1236-1244 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T28850 1366-1376 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T28289 10-131 Sentence denotes In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65.
T28290 132-784 Sentence denotes U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody.
T28291 785-1114 Sentence denotes Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T28292 1115-1245 Sentence denotes Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody.
T28293 1246-1596 Sentence denotes Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T28294 1597-1908 Sentence denotes Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T28295 1909-1996 Sentence denotes Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H).
T28296 1997-2078 Sentence denotes Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters.
T28297 2079-2115 Sentence denotes Values (mean ± SE, n = 3) are shown.
T28298 2116-2254 Sentence denotes The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P < 0.05).
T214 0-9 Sentence denotes Figure 6.
T215 10-131 Sentence denotes In HIV-1 infection Tat sustains the NF-κB activity and enhances the MIP-1α expression via interaction with IκB-α and p65.
T216 132-784 Sentence denotes U937 cells (5 × 107) were transfected with siRNA Tat, siRNA control (2 nmol), or left untransfected; 24 h later cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Real-time PCR of total RNA measured the Tat expression; ELISA measured the amount of p24 in whole cell extracts. (B) The binding of p65 to the NF-κB double-stranded oligonucleotide was measured in nuclear extracts (10 µg) using the NF-κB Transcription Factor ELISA assay kit (Cayman). (C) The IκB-α content was analysed by 12% SDS–PAGE and western blotting of cytosolic extracts (20 µg) using anti-IκB-α antibody.
T217 785-1114 Sentence denotes Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic cell extracts (100 µg) using HTScan IKK Kinase Assay (Cell Signaling Technology). (E) U937 cells (5 × 107) were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T218 1115-1245 Sentence denotes Twenty-four-hour post-infection, cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-FLAG antibody.
T219 1246-1596 Sentence denotes Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies. (F) U937 cells (5 × 107) were transfected with siRNA Tat, siRNA p65, siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T220 1597-1908 Sentence denotes Twenty-four-hour post-infection, total RNA was analysed for MIP-1α expression by real-time PCR. (G and H) U937 cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.FLAG-Tat.R−E− virions, or left uninfected.
T221 1909-1996 Sentence denotes Twenty-four-hour post-infection, ChIP was performed with anti-p65 (G) or anti-FLAG (H).
T222 1997-2078 Sentence denotes Real-time PCR was performed with primers specific for MIP-1α and GAPDH promoters.
T223 2079-2115 Sentence denotes Values (mean ± SE, n = 3) are shown.
T224 2116-2254 Sentence denotes The asterisks indicate statistically significant differences compared to the control (mock), according to the Student's t-test (P < 0.05).

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T28984 29-32 Protein denotes Tat
T28985 65-73 Positive_regulation denotes enhances
T28986 78-84 Protein denotes MIP-1α
T28987 85-95 Gene_expression denotes expression
T28988 100-111 Binding denotes interaction
T28989 117-122 Protein denotes IκB-α
T28990 127-130 Protein denotes p65
T28991 175-184 Protein denotes siRNA Tat
T28992 292-300 Protein denotes FLAG-Tat
T28993 325-328 Protein denotes p24
T28994 411-414 Protein denotes Tat
T28995 415-425 Gene_expression denotes expression true
T28996 456-459 Protein denotes p24
T28997 492-499 Binding denotes binding true
T28998 503-506 Protein denotes p65
T28999 664-669 Protein denotes IκB-α
T29000 1072-1080 Protein denotes FLAG-Tat
T29001 1198-1207 Protein denotes protein G
T29002 1425-1434 Protein denotes siRNA Tat
T29003 1436-1445 Protein denotes siRNA p65
T29004 1554-1562 Protein denotes FLAG-Tat
T29005 1657-1663 Protein denotes MIP-1α
T29006 1664-1674 Gene_expression denotes expression true
T29007 1746-1755 Protein denotes siRNA Tat
T29008 1866-1874 Protein denotes FLAG-Tat
T29009 2051-2057 Protein denotes MIP-1α
T29010 2062-2067 Protein denotes GAPDH
R22632 T28984 T28988 themeOf Tat,interaction
R22633 T28986 T28987 themeOf MIP-1α,expression
R22634 T28987 T28985 themeOf expression,enhances
R22635 T28988 T28985 causeOf interaction,enhances
R22636 T28989 T28988 themeOf IκB-α,interaction
R22637 T28994 T28995 themeOf Tat,expression
R22638 T28998 T28997 themeOf p65,binding
R22639 T29005 T29006 themeOf MIP-1α,expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T29011 46-51 Protein denotes NF-κB
T29012 78-84 Protein denotes MIP-1α
T29013 117-122 Protein denotes IκB-α
T29014 127-130 Protein denotes p65
T29015 85-95 Gene_expression denotes expression
T29016 100-111 Binding denotes interaction
T29017 65-73 Positive_regulation denotes enhances
T29018 175-184 Protein denotes siRNA Tat
T29019 325-328 Protein denotes p24
T29020 411-414 Protein denotes Tat
T29021 456-459 Protein denotes p24
T29022 415-425 Gene_expression denotes expression
T29023 503-506 Protein denotes p65
T29024 514-519 Protein denotes NF-κB
T29025 603-608 Protein denotes NF-κB
T29026 536-551 Entity denotes oligonucleotide
T29027 492-499 Binding denotes binding
T29028 664-669 Protein denotes IκB-α
T29029 764-783 Protein denotes anti-IκB-α antibody
T29030 758-763 Gene_expression denotes using
T29031 884-887 Protein denotes IKK
T29032 952-962 Protein denotes HTScan IKK
T29033 1198-1244 Protein denotes protein G-Sepharose-coupled anti-FLAG antibody
T29034 1336-1339 Protein denotes p65
T29035 1341-1350 Protein denotes anti-FLAG
T29036 1355-1376 Protein denotes anti-IκB-α antibodies
T29037 1425-1434 Protein denotes siRNA Tat
T29038 1436-1445 Protein denotes siRNA p65
T29039 1657-1663 Protein denotes MIP-1α
T29040 1664-1674 Gene_expression denotes expression
T29041 1746-1764 Protein denotes siRNA Tat or siRNA
T29042 2051-2057 Protein denotes MIP-1α
T29043 2062-2067 Protein denotes GAPDH
R22640 T29012 T29015 themeOf MIP-1α,expression
R22641 T29013 T29016 themeOf IκB-α,interaction
R22642 T29015 T29017 themeOf expression,enhances
R22643 T29016 T29017 causeOf interaction,enhances
R22644 T29020 T29022 themeOf Tat,expression
R22645 T29023 T29027 themeOf p65,binding
R22646 T29024 T29027 themeOf NF-κB,binding
R22647 T29024 T29026 partOf NF-κB,oligonucleotide
R22648 T29026 T29027 Site oligonucleotide,binding
R22649 T29029 T29030 themeOf anti-IκB-α antibody,using
R22650 T29039 T29040 themeOf MIP-1α,expression

test2

Id Subject Object Predicate Lexical cue Speculation
T28234 29-32 Protein denotes Tat
T28235 65-73 Positive_regulation denotes enhances
T28236 78-84 Protein denotes MIP-1α
T28237 85-95 Gene_expression denotes expression true
T28238 100-111 Binding denotes interaction true
T28239 117-122 Protein denotes IκB-α true
T28240 127-130 Protein denotes p65
T28241 175-184 Protein denotes siRNA Tat
T28242 292-300 Protein denotes FLAG-Tat
T28243 325-328 Protein denotes p24
T28244 411-414 Protein denotes Tat
T28245 415-425 Gene_expression denotes expression
T28246 456-459 Protein denotes p24
T28247 492-499 Binding denotes binding
T28248 503-506 Protein denotes p65
T28249 664-669 Protein denotes IκB-α
T28250 1072-1080 Protein denotes FLAG-Tat
T28251 1198-1207 Protein denotes protein G
T28252 1425-1434 Protein denotes siRNA Tat
T28253 1436-1445 Protein denotes siRNA p65
T28254 1554-1562 Protein denotes FLAG-Tat
T28255 1657-1663 Protein denotes MIP-1α
T28256 1664-1674 Gene_expression denotes expression
T28257 1746-1755 Protein denotes siRNA Tat
T28258 1866-1874 Protein denotes FLAG-Tat
T28259 2051-2057 Protein denotes MIP-1α
T28260 2062-2067 Protein denotes GAPDH
T28261 2068-2077 Entity denotes promoters
R22120 T28234 T28235 causeOf Tat,enhances
R22121 T28234 T28238 themeOf Tat,interaction
R22122 T28236 T28237 themeOf MIP-1α,expression
R22123 T28237 T28235 themeOf expression,enhances
R22124 T28238 T28235 causeOf interaction,enhances
R22125 T28239 T28238 themeOf IκB-α,interaction
R22126 T28244 T28245 themeOf Tat,expression
R22127 T28248 T28247 themeOf p65,binding
R22128 T28255 T28256 themeOf MIP-1α,expression

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T28262 29-32 Protein denotes Tat
T28263 65-73 Positive_regulation denotes enhances
T28264 78-84 Protein denotes MIP-1α
T28265 85-95 Gene_expression denotes expression
T28266 100-111 Binding denotes interaction
T28267 117-122 Protein denotes IκB-α
T28268 127-130 Protein denotes p65
T28269 175-184 Protein denotes siRNA Tat
T28270 292-300 Protein denotes FLAG-Tat
T28271 325-328 Protein denotes p24
T28272 411-414 Protein denotes Tat
T28273 415-425 Gene_expression denotes expression true
T28274 456-459 Protein denotes p24
T28275 492-499 Binding denotes binding true
T28276 503-506 Protein denotes p65
T28277 664-669 Protein denotes IκB-α
T28278 1072-1080 Protein denotes FLAG-Tat
T28279 1198-1207 Protein denotes protein G
T28280 1425-1434 Protein denotes siRNA Tat
T28281 1436-1445 Protein denotes siRNA p65
T28282 1554-1562 Protein denotes FLAG-Tat
T28283 1657-1663 Protein denotes MIP-1α
T28284 1664-1674 Gene_expression denotes expression true
T28285 1746-1755 Protein denotes siRNA Tat
T28286 1866-1874 Protein denotes FLAG-Tat
T28287 2051-2057 Protein denotes MIP-1α
T28288 2062-2067 Protein denotes GAPDH
R22129 T28262 T28266 themeOf Tat,interaction
R22130 T28264 T28265 themeOf MIP-1α,expression
R22131 T28265 T28263 themeOf expression,enhances
R22132 T28266 T28263 causeOf interaction,enhances
R22133 T28267 T28266 themeOf IκB-α,interaction
R22134 T28272 T28273 themeOf Tat,expression
R22135 T28276 T28275 themeOf p65,binding
R22136 T28283 T28284 themeOf MIP-1α,expression

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T28299 29-32 Q8UMQ1 denotes Tat
T28300 29-32 P04608 denotes Tat
T28301 29-32 P04610 denotes Tat
T28302 29-32 P04605 denotes Tat
T28303 78-84 P10147 denotes MIP-1α
T28304 127-130 P21579 denotes p65
T28305 127-130 Q04206 denotes p65
T28306 181-184 P04608 denotes Tat
T28307 181-184 P04605 denotes Tat
T28308 181-184 P04610 denotes Tat
T28309 181-184 Q8UMQ1 denotes Tat
T28310 297-300 P04608 denotes Tat
T28311 297-300 P04610 denotes Tat
T28312 297-300 Q8UMQ1 denotes Tat
T28313 297-300 P04605 denotes Tat
T28314 325-328 P04591 denotes p24
T28315 325-328 P12497 denotes p24
T28316 325-328 P03366 denotes p24
T28317 325-328 P04585 denotes p24
T28318 411-414 P04605 denotes Tat
T28319 411-414 Q8UMQ1 denotes Tat
T28320 411-414 P04610 denotes Tat
T28321 411-414 P04608 denotes Tat
T28322 456-459 P03366 denotes p24
T28323 456-459 P12497 denotes p24
T28324 456-459 P04591 denotes p24
T28325 456-459 P04585 denotes p24
T28326 503-506 Q04206 denotes p65
T28327 503-506 P21579 denotes p65
T28328 1077-1080 Q8UMQ1 denotes Tat
T28329 1077-1080 P04610 denotes Tat
T28330 1077-1080 P04608 denotes Tat
T28331 1077-1080 P04605 denotes Tat
T28332 1336-1339 Q04206 denotes p65
T28333 1336-1339 P21579 denotes p65
T28334 1431-1434 P04605 denotes Tat
T28335 1431-1434 P04608 denotes Tat
T28336 1431-1434 Q8UMQ1 denotes Tat
T28337 1431-1434 P04610 denotes Tat
T28338 1442-1445 Q04206 denotes p65
T28339 1442-1445 P21579 denotes p65
T28340 1559-1562 Q8UMQ1 denotes Tat
T28341 1559-1562 P04610 denotes Tat
T28342 1559-1562 P04605 denotes Tat
T28343 1559-1562 P04608 denotes Tat
T28344 1657-1663 P10147 denotes MIP-1α
T28345 1752-1755 P04608 denotes Tat
T28346 1752-1755 P04610 denotes Tat
T28347 1752-1755 P04605 denotes Tat
T28348 1752-1755 Q8UMQ1 denotes Tat
T28349 1871-1874 P04608 denotes Tat
T28350 1871-1874 P04610 denotes Tat
T28351 1871-1874 Q8UMQ1 denotes Tat
T28352 1871-1874 P04605 denotes Tat
T28353 1971-1974 P21579 denotes p65
T28354 1971-1974 Q04206 denotes p65
T28355 2051-2057 P10147 denotes MIP-1α
T28356 2062-2067 P04406 denotes GAPDH