PMC:3333881 / 14991-22494 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
22187158-12424381-77584445	3944-3946	12424381	denotes	17
22187158-15499023-77584446	3947-3949	15499023	denotes	18
22187158-17942396-77584447	6891-6893	17942396	denotes	50
22187158-21664225-77584448	6894-6896	21664225	denotes	51

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T8554	0-3	NN	denotes	Tat
T8555	4-12	VB	denotes	enhances
T8556	13-16	DT	denotes	the
T8557	17-22	NN	denotes	NF-κB
T8558	23-31	NN	denotes	activity
T8559	32-34	IN	denotes	by
T8560	35-44	VB	denotes	hijacking
T8561	45-50	NN	denotes	IκB-α
T8562	51-54	CC	denotes	and
T8563	55-65	VB	denotes	inhibiting
T8564	66-69	DT	denotes	the
T8565	70-85	JJ	denotes	post-activation
T8566	86-90	NN	denotes	turn
T8567	91-94	RP	denotes	off
T8568	95-97	IN	denotes	of
T8569	98-103	NN	denotes	NF-κB
T8570	104-106	PRP	denotes	We
T8571	107-115	VB	denotes	analysed
T8572	116-119	DT	denotes	the
T8573	120-127	NN	denotes	kinetic
T8574	128-130	IN	denotes	of
T8575	131-136	NN	denotes	NF-κB
T8576	137-147	NN	denotes	activation
T8577	148-150	IN	denotes	in
T8578	151-157	JJ	denotes	single
T8579	158-163	JJ	denotes	round
T8580	164-169	NN	denotes	HIV-1
T8581	170-179	NN	denotes	infection
T8582	180-182	IN	denotes	by
T8583	183-193	VB	denotes	modulating
T8584	194-197	DT	denotes	the
T8585	198-208	NN	denotes	expression
T8586	209-211	IN	denotes	of
T8587	212-215	NN	denotes	Tat
T8588	216-220	IN	denotes	with
T8589	221-224	NN	denotes	RNA
T8590	225-237	NN	denotes	interference
T8591	239-245	NN	denotes	Jurkat
T8592	246-251	NN	denotes	cells
T8593	252-256	VB	denotes	were
T8594	257-268	VB	denotes	transfected
T8595	269-273	IN	denotes	with
T8596	274-279	NN	denotes	siRNA
T8597	280-283	NN	denotes	Tat
T8598	283-284	-COMMA-	denotes	,
T8599	285-290	NN	denotes	siRNA
T8600	291-298	NN	denotes	control
T8601	298-299	-COMMA-	denotes	,
T8602	300-302	CC	denotes	or
T8603	303-307	VB	denotes	left
T8604	308-321	JJ	denotes	untransfected
T8605	321-322	-COMMA-	denotes	,
T8606	323-326	CC	denotes	and
T8607	327-331	NN	denotes	24 h
T8608	332-337	JJ	denotes	later
T8609	338-343	NN	denotes	cells
T8610	344-348	VB	denotes	were
T8611	349-357	VB	denotes	infected
T8612	358-362	IN	denotes	with
T8613	363-367	NN	denotes	HXB2
T8614	368-383	JJ	denotes	Env-pseudotyped
T8615	384-398	NN	denotes	NL4-3.Luc.R−E−
T8616	399-406	NN	denotes	virions
T8617	408-410	IN	denotes	By
T8618	411-427	JJ	denotes	cytofluorimetric
T8619	428-436	NN	denotes	analysis
T8620	436-437	-COMMA-	denotes	,
T8621	438-441	CD	denotes	∼40
T8622	441-442	NN	denotes	%
T8623	443-445	IN	denotes	of
T8624	446-451	NN	denotes	cells
T8625	452-455	VB	denotes	was
T8626	456-473	JJ	denotes	siRNA-transfected
T8627	474-477	CC	denotes	and
T8628	478-492	JJ	denotes	HIV-1-infected
T8629	493-495	IN	denotes	at
T8630	496-500	JJ	denotes	12-h
T8631	501-515	JJ	denotes	post-infection
T8632	516-517	-LRB-	denotes	(
T8633	517-530	JJ	denotes	Supplementary
T8634	531-537	NN	denotes	Figure
T8635	538-540	NN	denotes	S1
T8636	540-541	-RRB-	denotes	)
T8637	543-546	DT	denotes	The
T8638	547-557	NN	denotes	expression
T8639	558-560	IN	denotes	of
T8640	561-564	NN	denotes	Tat
T8641	565-568	VB	denotes	was
T8642	569-577	VB	denotes	detected
T8643	578-580	IN	denotes	at
T8644	581-584	JJ	denotes	1-h
T8645	585-599	JJ	denotes	post-infection
T8646	600-603	CC	denotes	and
T8647	604-617	RB	denotes	progressively
T8648	618-627	VB	denotes	increased
T8649	628-630	RB	denotes	up
T8650	631-633	TO	denotes	to
T8651	634-638	VB	denotes	12 h
T8652	639-641	IN	denotes	in
T8653	642-655	JJ	denotes	untransfected
T8654	656-659	CC	denotes	and
T8655	660-665	NN	denotes	siRNA
T8656	666-685	JJ	denotes	control-transfected
T8657	686-691	NN	denotes	cells
T8658	691-692	-COMMA-	denotes	,
T8659	693-698	IN	denotes	while
T8660	699-701	PRP	denotes	it
T8661	702-705	VB	denotes	was
T8662	706-712	RB	denotes	barely
T8663	713-721	VB	denotes	detected
T8664	722-724	IN	denotes	in
T8665	725-730	NN	denotes	siRNA
T8666	731-746	JJ	denotes	Tat-transfected
T8667	747-752	NN	denotes	cells
T8668	753-754	-LRB-	denotes	(
T8669	754-760	NN	denotes	Figure
T8670	761-763	NN	denotes	1A
T8671	763-764	-RRB-	denotes	)
T8672	766-769	DT	denotes	The
T8673	770-783	JJ	denotes	LTR-dependent
T8674	784-794	NN	denotes	luciferase
T8675	795-805	NN	denotes	expression
T8676	806-809	VB	denotes	was
T8677	810-814	RB	denotes	also
T8678	815-828	RB	denotes	progressively
T8679	829-836	VB	denotes	induced
T8680	837-839	IN	denotes	at
T8681	840-842	CD	denotes	1-
T8682	843-845	TO	denotes	to
T8683	846-850	JJ	denotes	12-h
T8684	851-865	NN	denotes	post-infection
T8685	866-868	IN	denotes	in
T8686	869-882	JJ	denotes	untransfected
T8687	883-886	CC	denotes	and
T8688	887-892	NN	denotes	siRNA
T8689	893-912	JJ	denotes	control-transfected
T8690	913-918	NN	denotes	cells
T8691	918-919	-COMMA-	denotes	,
T8692	920-925	IN	denotes	while
T8693	926-928	PRP	denotes	it
T8694	929-932	VB	denotes	was
T8695	933-939	RB	denotes	barely
T8696	940-948	VB	denotes	observed
T8697	949-951	IN	denotes	in
T8698	952-957	NN	denotes	siRNA
T8699	958-973	JJ	denotes	Tat-transfected
T8700	974-979	NN	denotes	cells
T8701	979-980	-COMMA-	denotes	,
T8702	981-983	IN	denotes	as
T8703	984-992	VB	denotes	expected
T8704	993-996	IN	denotes	for
T8705	997-1000	DT	denotes	the
T8706	1001-1014	JJ	denotes	Tat-dependent
T8707	1015-1030	NN	denotes	transactivation
T8708	1031-1033	IN	denotes	of
T8709	1034-1037	DT	denotes	the
T8710	1038-1043	NN	denotes	HIV-1
T8711	1044-1047	NN	denotes	LTR
T8712	1048-1049	-LRB-	denotes	(
T8713	1049-1055	NN	denotes	Figure
T8714	1056-1058	NN	denotes	1A
T8715	1058-1059	-RRB-	denotes	)
T8716	2254-2257	DT	denotes	The
T8717	2258-2269	JJ	denotes	progressive
T8718	2270-2278	NN	denotes	increase
T8719	2279-2281	IN	denotes	in
T8720	2282-2287	NN	denotes	NF-κB
T8721	2288-2296	NN	denotes	activity
T8722	2296-2297	-COMMA-	denotes	,
T8723	2298-2300	IN	denotes	as
T8724	2301-2309	VB	denotes	measured
T8725	2310-2312	IN	denotes	by
T8726	2313-2320	NN	denotes	p65–p50
T8727	2321-2328	NN	denotes	binding
T8728	2329-2331	TO	denotes	to
T8729	2332-2334	DT	denotes	an
T8730	2335-2340	NN	denotes	NF-κB
T8731	2341-2350	NN	denotes	consensus
T8732	2351-2366	NN	denotes	oligonucleotide
T8733	2367-2368	-LRB-	denotes	(
T8734	2368-2374	NN	denotes	Figure
T8735	2375-2377	NN	denotes	1B
T8736	2377-2378	-RRB-	denotes	)
T8737	2379-2382	CC	denotes	and
T8738	2383-2390	JJ	denotes	nuclear
T8739	2391-2394	NN	denotes	p65
T8740	2395-2396	-LRB-	denotes	(
T8741	2396-2402	NN	denotes	Figure
T8742	2403-2405	NN	denotes	1C
T8743	2405-2406	-COMMA-	denotes	,
T8744	2407-2414	NN	denotes	nucleus
T8745	2414-2415	-RRB-	denotes	)
T8746	2415-2416	-COMMA-	denotes	,
T8747	2417-2420	VB	denotes	was
T8748	2421-2429	VB	denotes	observed
T8749	2430-2432	IN	denotes	at
T8750	2433-2435	CD	denotes	1-
T8751	2436-2438	TO	denotes	to
T8752	2439-2442	JJ	denotes	3-h
T8753	2443-2457	NN	denotes	post-infection
T8754	2458-2460	IN	denotes	in
T8755	2461-2465	CC	denotes	both
T8756	2466-2478	JJ	denotes	Tat-positive
T8757	2479-2480	-LRB-	denotes	(
T8758	2480-2482	DT	denotes	no
T8759	2483-2488	NN	denotes	siRNA
T8760	2489-2491	CC	denotes	or
T8761	2492-2497	NN	denotes	siRNA
T8762	2498-2505	NN	denotes	control
T8763	2505-2506	-RRB-	denotes	)
T8764	2507-2510	CC	denotes	and
T8765	2511-2523	JJ	denotes	Tat-negative
T8766	2524-2525	-LRB-	denotes	(
T8767	2525-2530	NN	denotes	siRNA
T8768	2531-2534	NN	denotes	Tat
T8769	2534-2535	-RRB-	denotes	)
T8770	2536-2541	NN	denotes	cells
T8771	2541-2542	-COLON-	denotes	;
T8772	2543-2550	RB	denotes	however
T8773	2550-2551	-COMMA-	denotes	,
T8774	2552-2554	IN	denotes	at
T8775	2555-2559	JJ	denotes	12-h
T8776	2560-2574	NN	denotes	post-infection
T8777	2574-2575	-COMMA-	denotes	,
T8778	2576-2579	DT	denotes	the
T8779	2580-2583	NN	denotes	p65
T8780	2584-2587	NN	denotes	DNA
T8781	2588-2595	NN	denotes	binding
T8782	2596-2599	CC	denotes	and
T8783	2600-2607	JJ	denotes	nuclear
T8784	2608-2611	NN	denotes	p65
T8785	2612-2621	VB	denotes	persisted
T8786	2622-2630	VB	denotes	elevated
T8787	2631-2633	IN	denotes	in
T8788	2634-2642	NN	denotes	presence
T8789	2643-2645	IN	denotes	of
T8790	2646-2649	NN	denotes	Tat
T8791	2650-2651	-LRB-	denotes	(
T8792	2651-2653	DT	denotes	no
T8793	2654-2659	NN	denotes	siRNA
T8794	2660-2663	CC	denotes	and
T8795	2664-2669	NN	denotes	siRNA
T8796	2670-2677	NN	denotes	control
T8797	2677-2678	-RRB-	denotes	)
T8798	2678-2679	-COMMA-	denotes	,
T8799	2680-2685	IN	denotes	while
T8800	2686-2690	PRP	denotes	they
T8801	2691-2698	VB	denotes	dropped
T8802	2699-2701	IN	denotes	in
T8803	2702-2709	NN	denotes	absence
T8804	2710-2712	IN	denotes	of
T8805	2713-2716	NN	denotes	Tat
T8806	2717-2718	-LRB-	denotes	(
T8807	2718-2723	NN	denotes	siRNA
T8808	2724-2727	NN	denotes	Tat
T8809	2727-2728	-RRB-	denotes	)
T8810	2729-2730	-LRB-	denotes	(
T8811	2730-2736	NN	denotes	Figure
T8812	2737-2739	NN	denotes	1B
T8813	2740-2743	CC	denotes	and
T8814	2744-2745	NN	denotes	C
T8815	2745-2746	-RRB-	denotes	)
T8816	2748-2753	NN	denotes	IκB-α
T8817	2754-2757	VB	denotes	was
T8818	2758-2766	VB	denotes	degraded
T8819	2767-2769	IN	denotes	in
T8820	2770-2773	DT	denotes	the
T8821	2774-2781	NN	denotes	cytosol
T8822	2782-2788	IN	denotes	within
T8823	2789-2792	JJ	denotes	3-h
T8824	2793-2807	JJ	denotes	post-infection
T8825	2808-2811	CC	denotes	and
T8826	2812-2814	FW	denotes	de
T8827	2815-2819	FW	denotes	novo
T8828	2820-2831	VB	denotes	synthesized
T8829	2832-2834	IN	denotes	at
T8830	2835-2838	NN	denotes	6 h
T8831	2839-2843	IN	denotes	with
T8832	2844-2848	JJ	denotes	full
T8833	2849-2862	NN	denotes	replenishment
T8834	2863-2865	IN	denotes	at
T8835	2866-2870	JJ	denotes	12-h
T8836	2871-2885	NN	denotes	post-infection
T8837	2886-2888	IN	denotes	in
T8838	2889-2893	CC	denotes	both
T8839	2894-2906	JJ	denotes	Tat-positive
T8840	2907-2910	CC	denotes	and
T8841	2911-2923	JJ	denotes	Tat-negative
T8842	2924-2929	NN	denotes	cells
T8843	2930-2931	-LRB-	denotes	(
T8844	2931-2937	NN	denotes	Figure
T8845	2938-2940	NN	denotes	1C
T8846	2940-2941	-COMMA-	denotes	,
T8847	2942-2949	NN	denotes	cytosol
T8848	2949-2950	-RRB-	denotes	)
T8849	2952-2964	RB	denotes	Consistently
T8850	2964-2965	-COMMA-	denotes	,
T8851	2966-2969	DT	denotes	the
T8852	2970-2973	NN	denotes	IKK
T8853	2974-2982	NN	denotes	activity
T8854	2983-2986	VB	denotes	was
T8855	2987-2994	VB	denotes	induced
T8856	2995-2997	IN	denotes	at
T8857	2998-3001	JJ	denotes	1-h
T8858	3002-3016	NN	denotes	post-infection
T8859	3016-3017	-COMMA-	denotes	,
T8860	3018-3021	CC	denotes	and
T8861	3022-3031	VB	denotes	decreased
T8862	3032-3034	IN	denotes	at
T8863	3035-3038	JJ	denotes	3-h
T8864	3039-3053	JJ	denotes	post-infection
T8865	3054-3067	RB	denotes	independently
T8866	3068-3070	IN	denotes	of
T8867	3071-3074	DT	denotes	the
T8868	3075-3083	NN	denotes	presence
T8869	3084-3086	IN	denotes	of
T8870	3087-3090	NN	denotes	Tat
T8871	3091-3092	-LRB-	denotes	(
T8872	3092-3098	NN	denotes	Figure
T8873	3099-3101	NN	denotes	1D
T8874	3101-3102	-RRB-	denotes	)
T8875	3104-3111	JJ	denotes	Similar
T8876	3112-3120	NN	denotes	kinetics
T8877	3121-3123	IN	denotes	of
T8878	3124-3129	NN	denotes	NF-κB
T8879	3130-3138	NN	denotes	activity
T8880	3138-3139	-COMMA-	denotes	,
T8881	3140-3143	NN	denotes	IKK
T8882	3144-3154	NN	denotes	activation
T8883	3155-3158	CC	denotes	and
T8884	3159-3164	NN	denotes	IκB-α
T8885	3165-3189	NN	denotes	degradation/re-synthesis
T8886	3190-3194	VB	denotes	were
T8887	3195-3203	VB	denotes	observed
T8888	3204-3206	IN	denotes	in
T8889	3207-3219	JJ	denotes	single-round
T8890	3220-3225	NN	denotes	HIV-1
T8891	3226-3235	NN	denotes	infection
T8892	3236-3238	IN	denotes	of
T8893	3239-3244	NN	denotes	PBMCs
T8894	3245-3250	VB	denotes	using
T8895	3251-3252	DT	denotes	a
T8896	3253-3261	NN	denotes	cocktail
T8897	3262-3264	IN	denotes	of
T8898	3265-3279	NN	denotes	Azidothymidine
T8899	3280-3281	-LRB-	denotes	(
T8900	3281-3284	NN	denotes	AZT
T8901	3284-3285	-RRB-	denotes	)
T8902	3286-3289	CC	denotes	and
T8903	3290-3300	NN	denotes	Lamivudine
T8904	3301-3302	-LRB-	denotes	(
T8905	3302-3305	NN	denotes	3TC
T8906	3305-3306	-RRB-	denotes	)
T8907	3307-3309	IN	denotes	as
T8908	3310-3317	JJ	denotes	reverse
T8909	3318-3331	NN	denotes	transcriptase
T8910	3332-3342	NN	denotes	inhibitors
T8911	3343-3344	-LRB-	denotes	(
T8912	3344-3357	JJ	denotes	Supplementary
T8913	3358-3364	NNP	denotes	Figure
T8914	3365-3367	NN	denotes	S2
T8915	3367-3368	-RRB-	denotes	)
T8916	3370-3375	DT	denotes	These
T8917	3376-3383	NN	denotes	results
T8918	3384-3393	VB	denotes	indicated
T8919	3394-3398	IN	denotes	that
T8920	3399-3401	IN	denotes	in
T8921	3402-3414	JJ	denotes	single-round
T8922	3415-3420	NN	denotes	HIV-1
T8923	3421-3430	NN	denotes	infection
T8924	3431-3434	DT	denotes	the
T8925	3435-3440	NN	denotes	NF-κB
T8926	3441-3451	NN	denotes	activation
T8927	3452-3461	RB	denotes	initially
T8928	3462-3472	VB	denotes	correlated
T8929	3473-3477	IN	denotes	with
T8930	3478-3481	DT	denotes	the
T8931	3482-3485	NN	denotes	IKK
T8932	3486-3496	NN	denotes	activation
T8933	3497-3500	CC	denotes	and
T8934	3501-3506	JJ	denotes	IκB-α
T8935	3507-3518	NN	denotes	degradation
T8936	3519-3532	RB	denotes	independently
T8937	3533-3535	IN	denotes	of
T8938	3536-3539	DT	denotes	the
T8939	3540-3548	NN	denotes	presence
T8940	3549-3551	IN	denotes	of
T8941	3552-3555	NN	denotes	Tat
T8942	3555-3556	-COMMA-	denotes	,
T8943	3557-3560	CC	denotes	and
T8944	3561-3563	PRP	denotes	it
T8945	3564-3567	VB	denotes	was
T8946	3568-3572	VB	denotes	kept
T8947	3573-3581	VB	denotes	elevated
T8948	3582-3584	IN	denotes	in
T8949	3585-3593	NN	denotes	presence
T8950	3594-3596	IN	denotes	of
T8951	3597-3600	NN	denotes	Tat
T8952	3601-3610	VB	denotes	following
T8953	3611-3614	DT	denotes	the
T8954	3615-3620	NN	denotes	decay
T8955	3621-3623	IN	denotes	of
T8956	3624-3627	DT	denotes	the
T8957	3628-3631	NN	denotes	IKK
T8958	3632-3640	NN	denotes	activity
T8959	3641-3644	CC	denotes	and
T8960	3645-3648	JJ	denotes	new
T8961	3649-3658	NN	denotes	synthesis
T8962	3659-3661	IN	denotes	of
T8963	3662-3667	NN	denotes	IκB-α
T8964	3669-3673	RB	denotes	Next
T8965	3673-3674	-COMMA-	denotes	,
T8966	3675-3677	PRP	denotes	we
T8967	3678-3684	VB	denotes	tested
T8968	3685-3688	DT	denotes	the
T8969	3689-3695	JJ	denotes	single
T8970	3696-3702	NN	denotes	action
T8971	3703-3705	IN	denotes	of
T8972	3706-3709	NN	denotes	Tat
T8973	3710-3712	IN	denotes	on
T8974	3713-3716	DT	denotes	the
T8975	3717-3724	NN	denotes	kinetic
T8976	3725-3727	IN	denotes	of
T8977	3728-3733	NN	denotes	NF-κB
T8978	3734-3742	NN	denotes	activity
T8979	3743-3750	VB	denotes	induced
T8980	3751-3753	IN	denotes	by
T8981	3754-3757	NN	denotes	PMA
T8982	3759-3761	TO	denotes	To
T8983	3762-3766	DT	denotes	this
T8984	3767-3770	NN	denotes	end
T8985	3770-3771	-COMMA-	denotes	,
T8986	3772-3776	NN	denotes	HeLa
T8987	3777-3782	NN	denotes	cells
T8988	3783-3787	VB	denotes	were
T8989	3788-3799	VB	denotes	transfected
T8990	3800-3804	IN	denotes	with
T8991	3805-3817	NN	denotes	p3x-FLAG-Tat
T8992	3817-3818	-COMMA-	denotes	,
T8993	3819-3821	CC	denotes	or
T8994	3822-3827	JJ	denotes	empty
T8995	3828-3834	NN	denotes	vector
T8996	3834-3835	-COMMA-	denotes	,
T8997	3836-3846	VB	denotes	stimulated
T8998	3847-3851	IN	denotes	with
T8999	3852-3855	NN	denotes	PMA
T9000	3856-3859	IN	denotes	for
T9001	3860-3865	NN	denotes	5 min
T9002	3865-3866	-COMMA-	denotes	,
T9003	3867-3870	CC	denotes	and
T9004	3871-3882	RB	denotes	extensively
T9005	3883-3889	VB	denotes	washed
T9006	3890-3892	TO	denotes	to
T9007	3893-3898	VB	denotes	avoid
T9008	3899-3902	DT	denotes	the
T9009	3903-3914	JJ	denotes	oscillatory
T9010	3915-3922	NN	denotes	kinetic
T9011	3923-3925	IN	denotes	of
T9012	3926-3931	NN	denotes	NF-κB
T9013	3932-3942	NN	denotes	activation
T9014	3943-3944	-LRB-	denotes	(
T9015	3944-3949	CD	denotes	17,18
T9016	3949-3950	-RRB-	denotes	)
T9017	3952-3954	IN	denotes	In
T9018	3955-3968	JJ	denotes	un-stimulated
T9019	3969-3974	NN	denotes	cells
T9020	3974-3975	-COMMA-	denotes	,
T9021	3976-3979	DT	denotes	the
T9022	3980-3983	NN	denotes	p65
T9023	3984-3987	NN	denotes	DNA
T9024	3988-3995	NN	denotes	binding
T9025	3996-3997	-LRB-	denotes	(
T9026	3997-4003	NN	denotes	Figure
T9027	4004-4006	NN	denotes	2A
T9028	4006-4007	-RRB-	denotes	)
T9029	4008-4011	CC	denotes	and
T9030	4012-4019	JJ	denotes	nuclear
T9031	4020-4023	NN	denotes	p65
T9032	4024-4025	-LRB-	denotes	(
T9033	4025-4031	NN	denotes	Figure
T9034	4032-4034	NN	denotes	2B
T9035	4034-4035	-COMMA-	denotes	,
T9036	4036-4043	NN	denotes	nucleus
T9037	4043-4044	-RRB-	denotes	)
T9038	4045-4049	VB	denotes	were
T9039	4050-4058	RB	denotes	slightly
T9040	4059-4067	VB	denotes	enhanced
T9041	4068-4070	IN	denotes	by
T9042	4071-4074	NN	denotes	Tat
T9043	4074-4075	-COMMA-	denotes	,
T9044	4076-4081	IN	denotes	while
T9045	4082-4085	DT	denotes	the
T9046	4086-4091	JJ	denotes	IκB-α
T9047	4092-4099	NN	denotes	content
T9048	4100-4103	VB	denotes	was
T9049	4104-4107	RB	denotes	not
T9050	4108-4121	RB	denotes	significantly
T9051	4122-4130	VB	denotes	affected
T9052	4131-4132	-LRB-	denotes	(
T9053	4132-4138	NN	denotes	Figure
T9054	4139-4141	NN	denotes	2B
T9055	4141-4142	-COMMA-	denotes	,
T9056	4143-4150	NN	denotes	cytosol
T9057	4150-4151	-RRB-	denotes	)
T9058	4153-4162	JJ	denotes	Transient
T9059	4163-4174	NN	denotes	stimulation
T9060	4175-4179	IN	denotes	with
T9061	4180-4183	NN	denotes	PMA
T9062	4184-4193	VB	denotes	increased
T9063	4194-4197	DT	denotes	the
T9064	4198-4203	NN	denotes	NF-κB
T9065	4204-4207	NN	denotes	DNA
T9066	4208-4215	NN	denotes	binding
T9067	4216-4224	NN	denotes	activity
T9068	4225-4228	CC	denotes	and
T9069	4229-4236	JJ	denotes	nuclear
T9070	4237-4240	NN	denotes	p65
T9071	4241-4243	IN	denotes	at
T9072	4244-4249	NN	denotes	5 min
T9073	4250-4257	VB	denotes	peaking
T9074	4258-4260	IN	denotes	at
T9075	4261-4271	NN	denotes	60–120 min
T9076	4272-4285	RB	denotes	independently
T9077	4286-4288	IN	denotes	of
T9078	4289-4292	DT	denotes	the
T9079	4293-4301	NN	denotes	presence
T9080	4302-4304	IN	denotes	of
T9081	4305-4308	NN	denotes	Tat
T9082	4309-4310	-LRB-	denotes	(
T9083	4310-4316	NN	denotes	Figure
T9084	4317-4319	NN	denotes	2A
T9085	4320-4323	CC	denotes	and
T9086	4324-4325	NN	denotes	B
T9087	4325-4326	-RRB-	denotes	)
T9088	4326-4327	-COLON-	denotes	;
T9089	4328-4335	RB	denotes	however
T9090	4335-4336	-COMMA-	denotes	,
T9091	4337-4342	IN	denotes	while
T9092	4343-4346	DT	denotes	the
T9093	4347-4350	NN	denotes	p65
T9094	4351-4354	NN	denotes	DNA
T9095	4355-4362	NN	denotes	binding
T9096	4363-4366	CC	denotes	and
T9097	4367-4374	JJ	denotes	nuclear
T9098	4375-4378	NN	denotes	p65
T9099	4379-4388	VB	denotes	persisted
T9100	4389-4397	VB	denotes	elevated
T9101	4398-4400	IN	denotes	at
T9102	4401-4408	JJ	denotes	240-min
T9103	4409-4423	JJ	denotes	post-treatment
T9104	4424-4426	IN	denotes	in
T9105	4427-4439	JJ	denotes	Tat-positive
T9106	4440-4445	NN	denotes	cells
T9107	4445-4446	-COMMA-	denotes	,
T9108	4447-4451	PRP	denotes	they
T9109	4452-4459	VB	denotes	dropped
T9110	4460-4462	IN	denotes	in
T9111	4463-4475	JJ	denotes	Tat-negative
T9112	4476-4481	NN	denotes	cells
T9113	4482-4483	-LRB-	denotes	(
T9114	4483-4489	NN	denotes	Figure
T9115	4490-4492	NN	denotes	2A
T9116	4493-4496	CC	denotes	and
T9117	4497-4498	NN	denotes	B
T9118	4498-4499	-RRB-	denotes	)
T9119	4501-4503	IN	denotes	In
T9120	4504-4512	NN	denotes	addition
T9121	4512-4513	-COMMA-	denotes	,
T9122	4514-4517	NN	denotes	Tat
T9123	4518-4523	VB	denotes	bound
T9124	4524-4526	TO	denotes	to
T9125	4527-4530	DT	denotes	the
T9126	4531-4536	NN	denotes	NF-κB
T9127	4537-4552	NN	denotes	oligonucleotide
T9128	4553-4555	IN	denotes	in
T9129	4556-4569	JJ	denotes	un-stimulated
T9130	4570-4575	NN	denotes	cells
T9131	4576-4579	CC	denotes	and
T9132	4580-4583	PRP-DOLLAR-	denotes	its
T9133	4584-4591	NN	denotes	binding
T9134	4592-4601	VB	denotes	increased
T9135	4602-4611	VB	denotes	following
T9136	4612-4615	NN	denotes	PMA
T9137	4616-4627	NN	denotes	stimulation
T9138	4628-4629	-LRB-	denotes	(
T9139	4629-4635	NN	denotes	Figure
T9140	4636-4638	NN	denotes	2A
T9141	4638-4639	-RRB-	denotes	)
T9142	4639-4640	-COMMA-	denotes	,
T9143	4641-4651	VB	denotes	suggesting
T9144	4652-4656	IN	denotes	that
T9145	4657-4660	DT	denotes	the
T9146	4661-4666	JJ	denotes	viral
T9147	4667-4674	NN	denotes	protein
T9148	4675-4678	VB	denotes	was
T9149	4679-4680	DT	denotes	a
T9150	4681-4690	NN	denotes	component
T9151	4691-4693	IN	denotes	of
T9152	4694-4697	DT	denotes	the
T9153	4698-4703	NN	denotes	NF-κB
T9154	4704-4711	NN	denotes	complex
T9155	4712-4717	VB	denotes	bound
T9156	4718-4720	TO	denotes	to
T9157	4721-4724	NN	denotes	DNA
T9158	4726-4737	NN	denotes	Degradation
T9159	4738-4740	IN	denotes	of
T9160	4741-4746	NN	denotes	IκB-α
T9161	4747-4750	VB	denotes	was
T9162	4751-4764	RB	denotes	progressively
T9163	4765-4772	VB	denotes	induced
T9164	4773-4775	IN	denotes	at
T9165	4776-4784	NN	denotes	5–30 min
T9166	4784-4785	-COMMA-	denotes	,
T9167	4786-4794	VB	denotes	followed
T9168	4795-4797	IN	denotes	by
T9169	4798-4800	FW	denotes	de
T9170	4801-4805	FW	denotes	novo
T9171	4806-4815	NN	denotes	synthesis
T9172	4816-4818	IN	denotes	at
T9173	4819-4825	NN	denotes	60 min
T9174	4826-4830	IN	denotes	with
T9175	4831-4835	JJ	denotes	full
T9176	4836-4849	NN	denotes	replenishment
T9177	4850-4852	IN	denotes	at
T9178	4853-4860	NN	denotes	240 min
T9179	4861-4863	IN	denotes	in
T9180	4864-4868	CC	denotes	both
T9181	4869-4881	JJ	denotes	Tat-positive
T9182	4882-4885	CC	denotes	and
T9183	4886-4894	JJ	denotes	negative
T9184	4895-4900	NN	denotes	cells
T9185	4901-4902	-LRB-	denotes	(
T9186	4902-4908	NN	denotes	Figure
T9187	4909-4911	NN	denotes	2B
T9188	4911-4912	-COMMA-	denotes	,
T9189	4913-4920	NN	denotes	cytosol
T9190	4920-4921	-RRB-	denotes	)
T9191	4923-4935	RB	denotes	Consistently
T9192	4935-4936	-COMMA-	denotes	,
T9193	4937-4940	DT	denotes	the
T9194	4941-4944	NN	denotes	IKK
T9195	4945-4955	NN	denotes	activation
T9196	4956-4963	VB	denotes	started
T9197	4964-4966	IN	denotes	at
T9198	4967-4972	NN	denotes	5 min
T9199	4972-4973	-COMMA-	denotes	,
T9200	4974-4977	CC	denotes	and
T9201	4978-4984	VB	denotes	turned
T9202	4985-4988	RP	denotes	off
T9203	4989-4991	IN	denotes	at
T9204	4992-4998	CD	denotes	30 min
T9205	4999-5012	RB	denotes	independently
T9206	5013-5015	IN	denotes	of
T9207	5016-5019	DT	denotes	the
T9208	5020-5028	NN	denotes	presence
T9209	5029-5031	IN	denotes	of
T9210	5032-5035	NN	denotes	Tat
T9211	5036-5037	-LRB-	denotes	(
T9212	5037-5043	NN	denotes	Figure
T9213	5044-5046	NN	denotes	2C
T9214	5046-5047	-RRB-	denotes	)
T9215	5049-5054	DT	denotes	These
T9216	5055-5062	NN	denotes	results
T9217	5063-5072	VB	denotes	indicated
T9218	5073-5077	IN	denotes	that
T9219	5078-5081	NN	denotes	Tat
T9220	5082-5090	VB	denotes	enhanced
T9221	5091-5094	DT	denotes	the
T9222	5095-5100	NN	denotes	NF-κB
T9223	5101-5109	NN	denotes	activity
T9224	5110-5112	IN	denotes	in
T9225	5113-5126	JJ	denotes	un-stimulated
T9226	5127-5130	CC	denotes	and
T9227	5131-5145	JJ	denotes	PMA-stimulated
T9228	5146-5151	NN	denotes	cells
T9229	5152-5159	IN	denotes	without
T9230	5160-5169	VB	denotes	affecting
T9231	5170-5173	DT	denotes	the
T9232	5174-5177	NN	denotes	IKK
T9233	5178-5186	NN	denotes	activity
T9234	5187-5190	CC	denotes	and
T9235	5191-5196	JJ	denotes	IκB-α
T9236	5197-5204	NN	denotes	content
T9237	6822-6824	IN	denotes	As
T9238	6825-6827	PRP	denotes	we
T9239	6828-6838	RB	denotes	previously
T9240	6839-6844	VB	denotes	found
T9241	6845-6849	IN	denotes	that
T9242	6850-6853	NN	denotes	Tat
T9243	6854-6859	VB	denotes	binds
T9244	6860-6862	TO	denotes	to
T9245	6863-6866	DT	denotes	the
T9246	6867-6872	JJ	denotes	sixth
T9247	6873-6880	NN	denotes	ankyrin
T9248	6881-6883	IN	denotes	of
T9249	6884-6889	NN	denotes	IκB-α
T9250	6890-6891	-LRB-	denotes	(
T9251	6891-6896	CD	denotes	50,51
T9252	6896-6897	-RRB-	denotes	)
T9253	6897-6898	-COMMA-	denotes	,
T9254	6899-6901	PRP	denotes	we
T9255	6902-6908	VB	denotes	tested
T9256	6909-6916	IN	denotes	whether
T9257	6917-6920	NN	denotes	Tat
T9258	6921-6933	VB	denotes	counteracted
T9259	6934-6937	DT	denotes	the
T9260	6938-6948	NN	denotes	generation
T9261	6949-6951	IN	denotes	of
T9262	6952-6955	DT	denotes	the
T9263	6956-6967	NN	denotes	IκB-α/NF-κB
T9264	6968-6975	NN	denotes	complex
T9265	6977-6979	TO	denotes	To
T9266	6980-6984	DT	denotes	this
T9267	6985-6988	NN	denotes	end
T9268	6988-6989	-COMMA-	denotes	,
T9269	6990-6993	DT	denotes	the
T9270	6994-7005	NN	denotes	association
T9271	7006-7008	IN	denotes	of
T9272	7009-7014	NN	denotes	IκB-α
T9273	7015-7019	IN	denotes	with
T9274	7020-7023	NN	denotes	p65
T9275	7024-7027	VB	denotes	was
T9276	7028-7036	VB	denotes	analysed
T9277	7037-7039	FW	denotes	in
T9278	7040-7044	FW	denotes	vivo
T9279	7045-7047	IN	denotes	at
T9280	7048-7049	CD	denotes	0
T9281	7050-7053	CC	denotes	and
T9282	7054-7061	NN	denotes	240 min
T9283	7062-7067	IN	denotes	after
T9284	7068-7079	JJ	denotes	short-pulse
T9285	7080-7083	NN	denotes	PMA
T9286	7083-7084	-COMMA-	denotes	,
T9287	7085-7090	WDT	denotes	which
T9288	7091-7103	VB	denotes	corresponded
T9289	7104-7106	TO	denotes	to
T9290	7107-7110	DT	denotes	the
T9291	7111-7115	NN	denotes	time
T9292	7116-7118	IN	denotes	of
T9293	7119-7132	JJ	denotes	un-stimulated
T9294	7133-7142	NN	denotes	condition
T9295	7143-7146	CC	denotes	and
T9296	7147-7162	JJ	denotes	post-activation
T9297	7163-7165	FW	denotes	de
T9298	7166-7170	FW	denotes	novo
T9299	7171-7180	NN	denotes	synthesis
T9300	7181-7183	IN	denotes	of
T9301	7184-7189	NN	denotes	IκB-α
T9302	7189-7190	-COMMA-	denotes	,
T9303	7191-7203	RB	denotes	respectively
T9304	7205-7226	NN	denotes	Coimmunoprecipitation
T9305	7227-7229	IN	denotes	of
T9306	7230-7235	NN	denotes	IκB-α
T9307	7236-7240	IN	denotes	with
T9308	7241-7244	NN	denotes	p65
T9309	7245-7248	VB	denotes	was
T9310	7249-7257	VB	denotes	detected
T9311	7258-7260	IN	denotes	at
T9312	7261-7263	CD	denotes	0-
T9313	7264-7267	CC	denotes	and
T9314	7268-7275	NN	denotes	240-min
T9315	7276-7290	JJ	denotes	post-treatment
T9316	7291-7293	IN	denotes	in
T9317	7294-7306	JJ	denotes	Tat-negative
T9318	7307-7312	NN	denotes	cells
T9319	7313-7314	-LRB-	denotes	(
T9320	7314-7320	NN	denotes	Figure
T9321	7321-7323	NN	denotes	2D
T9322	7323-7324	-COMMA-	denotes	,
T9323	7325-7330	NN	denotes	lanes
T9324	7331-7332	CD	denotes	1
T9325	7333-7336	CC	denotes	and
T9326	7337-7338	CD	denotes	3
T9327	7338-7339	-RRB-	denotes	)
T9328	7339-7340	-COMMA-	denotes	,
T9329	7341-7344	CC	denotes	and
T9330	7345-7348	VB	denotes	was
T9331	7349-7355	VB	denotes	halved
T9332	7356-7358	IN	denotes	in
T9333	7359-7371	JJ	denotes	Tat-positive
T9334	7372-7377	NN	denotes	cells
T9335	7377-7378	-COMMA-	denotes	,
T9336	7379-7384	WRB	denotes	where
T9337	7385-7388	NN	denotes	Tat
T9338	7389-7409	VB	denotes	coimmunoprecipitated
T9339	7410-7414	IN	denotes	with
T9340	7415-7420	NN	denotes	IκB-α
T9341	7421-7422	-LRB-	denotes	(
T9342	7422-7428	NN	denotes	Figure
T9343	7429-7431	NN	denotes	2D
T9344	7431-7432	-COMMA-	denotes	,
T9345	7433-7438	NN	denotes	lanes
T9346	7439-7440	CD	denotes	2
T9347	7441-7444	CC	denotes	and
T9348	7445-7446	CD	denotes	4
T9349	7446-7447	-RRB-	denotes	)
T9350	7447-7448	-COMMA-	denotes	,
T9351	7449-7459	VB	denotes	indicating
T9352	7460-7464	IN	denotes	that
T9353	7465-7468	NN	denotes	Tat
T9354	7469-7477	VB	denotes	competed
T9355	7478-7481	DT	denotes	the
T9356	7482-7487	JJ	denotes	IκB-α
T9357	7488-7495	NN	denotes	binding
T9358	7496-7498	TO	denotes	to
T9359	7499-7502	NN	denotes	p65
T24359	5216-5219	NN	denotes	Tat
T24360	5220-5228	VB	denotes	enhances
T24361	5229-5232	DT	denotes	the
T24362	5233-5238	NN	denotes	NF-κB
T24363	5239-5247	NN	denotes	activity
T24364	5248-5257	VB	denotes	following
T24365	5258-5261	NN	denotes	PMA
T24366	5262-5273	NN	denotes	stimulation
T24367	5274-5276	IN	denotes	by
T24368	5277-5288	VB	denotes	associating
T24369	5289-5293	IN	denotes	with
T24370	5294-5299	NN	denotes	IκB-α
T24371	5300-5303	CC	denotes	and
T24372	5304-5317	VB	denotes	counteracting
T24373	5318-5321	DT	denotes	the
T24374	5322-5327	NN	denotes	NF-κB
T24375	5328-5338	NN	denotes	repression
T24376	5340-5344	NN	denotes	HeLa
T24377	5345-5350	NN	denotes	cells
T24378	5351-5352	-LRB-	denotes	(
T24379	5352-5359	CD	denotes	5 × 106
T24380	5359-5360	-RRB-	denotes	)
T24381	5361-5365	VB	denotes	were
T24382	5366-5377	VB	denotes	transfected
T24383	5378-5382	IN	denotes	with
T24384	5383-5394	NN	denotes	p3xFLAG-Tat
T24385	5395-5397	CC	denotes	or
T24386	5398-5405	NN	denotes	p3xFLAG
T24387	5406-5411	NN	denotes	empty
T24388	5412-5418	NN	denotes	vector
T24389	5419-5420	-LRB-	denotes	(
T24390	5420-5424	NN	denotes	5 µg
T24391	5424-5425	-RRB-	denotes	)
T24392	5425-5426	-COMMA-	denotes	,
T24393	5427-5430	CC	denotes	and
T24394	5431-5435	NN	denotes	48 h
T24395	5436-5441	RB	denotes	later
T24396	5442-5446	VB	denotes	were
T24397	5447-5457	VB	denotes	stimulated
T24398	5458-5462	IN	denotes	with
T24399	5463-5466	NN	denotes	PMA
T24400	5467-5468	-LRB-	denotes	(
T24401	5468-5473	NN	denotes	5 min
T24402	5473-5474	-COMMA-	denotes	,
T24403	5475-5483	NN	denotes	20 ng/ml
T24404	5483-5484	-RRB-	denotes	)
T24405	5485-5487	CC	denotes	or
T24406	5488-5492	VB	denotes	left
T24407	5493-5505	JJ	denotes	unstimulated
T24408	5505-5506	-COMMA-	denotes	,
T24409	5507-5513	VB	denotes	washed
T24410	5514-5519	RB	denotes	twice
T24411	5520-5524	IN	denotes	with
T24412	5525-5529	NN	denotes	DMEM
T24413	5530-5533	CC	denotes	and
T24414	5534-5543	VB	denotes	harvested
T24415	5544-5546	IN	denotes	at
T24416	5547-5550	DT	denotes	the
T24417	5551-5560	JJ	denotes	indicated
T24418	5561-5566	NN	denotes	times
T24419	5568-5575	JJ	denotes	Nuclear
T24420	5576-5579	CC	denotes	and
T24421	5580-5589	JJ	denotes	cytosolic
T24422	5590-5598	NN	denotes	extracts
T24423	5599-5603	VB	denotes	were
T24424	5604-5612	VB	denotes	prepared
T24425	5613-5616	IN	denotes	for
T24426	5617-5624	JJ	denotes	further
T24427	5625-5634	NN	denotes	analysis.
T24428	5635-5636	-LRB-	denotes	(
T24429	5636-5637	NN	denotes	A
T24430	5637-5638	-RRB-	denotes	)
T24431	5639-5646	JJ	denotes	Nuclear
T24432	5647-5655	NN	denotes	extracts
T24433	5656-5660	VB	denotes	were
T24434	5661-5669	VB	denotes	analysed
T24435	5670-5673	IN	denotes	for
T24436	5674-5677	DT	denotes	the
T24437	5678-5681	NN	denotes	p65
T24438	5681-5682	-COMMA-	denotes	,
T24439	5683-5686	NN	denotes	p50
T24440	5687-5690	CC	denotes	and
T24441	5691-5699	NN	denotes	FLAG-Tat
T24442	5700-5707	NN	denotes	binding
T24443	5708-5710	TO	denotes	to
T24444	5711-5714	DT	denotes	the
T24445	5715-5720	NN	denotes	NF-κB
T24446	5721-5736	JJ	denotes	double-stranded
T24447	5737-5752	NN	denotes	oligonucleotide
T24448	5753-5758	VB	denotes	using
T24449	5759-5762	DT	denotes	the
T24450	5763-5768	NN	denotes	NF-κB
T24451	5769-5782	NN	denotes	Transcription
T24452	5783-5789	NN	denotes	Factor
T24453	5790-5795	NN	denotes	ELISA
T24454	5796-5801	NN	denotes	assay
T24455	5802-5805	NN	denotes	kit
T24456	5806-5807	-LRB-	denotes	(
T24457	5807-5813	NN	denotes	Cayman
T24458	5813-5814	-RRB-	denotes	)
T24459	5814-5815	-LRB-	denotes	.
T24460	5816-5817	NN	denotes	(
T24461	5817-5818	-RRB-	denotes	B
T24462	5818-5825	JJ	denotes	) Nucle
T24463	5825-5828	CC	denotes	ar
T24464	5828-5837	JJ	denotes	and cytos
T24465	5837-5845	NN	denotes	olic ext
T24466	5845-5846	-LRB-	denotes	r
T24467	5846-5851	NN	denotes	acts
T24468	5851-5852	-RRB-	denotes	(
T24469	5852-5856	VB	denotes	20 µ
T24470	5856-5865	VB	denotes	g) were s
T24471	5865-5867	IN	denotes	ep
T24472	5867-5869	CD	denotes	ar
T24473	5869-5870	NN	denotes	a
T24474	5870-5878	NN	denotes	ted by 1
T24475	5878-5881	CC	denotes	2%
T24476	5881-5889	VB	denotes	SDS–PAGE
T24477	5890-5892	IN	denotes	an
T24478	5892-5899	JJ	denotes	d analy
T24479	5899-5907	NN	denotes	sed by w
T24480	5907-5912	VB	denotes	ester
T24481	5912-5915	DT	denotes	n b
T24482	5915-5923	JJ	denotes	lotting
T24483	5923-5924	-COMMA-	denotes	u
T24484	5924-5933	JJ	denotes	sing the
T24485	5933-5934	-COMMA-	denotes	a
T24486	5934-5946	JJ	denotes	nti-p65, ant
T24487	5946-5948	NN	denotes	i-
T24488	5948-5949	-COMMA-	denotes	F
T24489	5949-5959	JJ	denotes	LAG, anti-
T24490	5959-5962	CC	denotes	His
T24491	5962-5980	JJ	denotes	tone H1, anti-IκB-
T24492	5980-5990	NN	denotes	α and anti
T24493	6017-6029	NN	denotes	Densitometry
T24494	6030-6036	NN	denotes	values
T24495	6037-6038	-LRB-	denotes	(
T24496	6038-6039	NN	denotes	D
T24497	6039-6040	-RRB-	denotes	)
T24498	6041-6043	IN	denotes	of
T24499	6044-6047	DT	denotes	the
T24500	6048-6053	NN	denotes	bands
T24501	6054-6058	VB	denotes	were
T24502	6059-6068	VB	denotes	expressed
T24503	6069-6071	IN	denotes	as
T24504	6072-6076	JJ	denotes	fold
T24505	6077-6085	NN	denotes	increase
T24506	6086-6091	IN	denotes	above
T24507	6092-6095	DT	denotes	the
T24508	6096-6103	NN	denotes	control
T24509	6104-6105	-LRB-	denotes	(
T24510	6105-6109	NN	denotes	lane
T24511	6110-6111	CD	denotes	1
T24512	6111-6112	-RRB-	denotes	)
T24513	6112-6113	-LRB-	denotes	.
T24514	6114-6115	NN	denotes	(
T24515	6115-6116	-RRB-	denotes	C
T24516	6116-6119	NN	denotes	) I
T24517	6119-6127	NN	denotes	KK activ
T24518	6127-6130	VB	denotes	ity
T24519	6131-6139	VB	denotes	was meas
T24520	6139-6141	IN	denotes	ur
T24521	6141-6150	JJ	denotes	ed in cyt
T24522	6150-6158	NN	denotes	osolic e
T24523	6158-6159	-LRB-	denotes	x
T24524	6159-6165	NN	denotes	tracts
T24525	6166-6167	-RRB-	denotes	(
T24526	6167-6169	IN	denotes	10
T24527	6169-6174	VB	denotes	0 µg)
T24528	6175-6181	NN	denotes	by usi
T24529	6181-6184	NN	denotes	ng
T24530	6184-6190	NN	denotes	HTScan
T24531	6191-6196	NN	denotes	IKK k
T24532	6196-6197	-LRB-	denotes	i
T24533	6197-6201	NNP	denotes	nase
T24534	6202-6211	NNP	denotes	assay (Ce
T24535	6211-6221	NNP	denotes	ll Signali
T24536	6221-6222	-RRB-	denotes	n
T24537	6222-6223	-LRB-	denotes	g
T24538	6224-6225	NN	denotes	T
T24539	6225-6226	-RRB-	denotes	e
T24540	6226-6230	NN	denotes	chno
T24541	6230-6235	NN	denotes	logy)
T24542	6235-6236	-LRB-	denotes	.
T24543	6237-6244	CD	denotes	(D) HeL
T24544	6244-6245	-RRB-	denotes	a
T24545	6246-6250	VB	denotes	cell
T24546	6250-6261	VB	denotes	s (5 × 106)
T24547	6262-6266	IN	denotes	were
T24548	6267-6269	CC	denotes	tr
T24549	6269-6276	IN	denotes	ansfect
T24550	6276-6287	NN	denotes	ed with or
T24551	6287-6288	-LRB-	denotes	w
T24552	6288-6292	NN	denotes	itho
T24553	6292-6293	-RRB-	denotes	u
T24554	6293-6294	-COLON-	denotes	t
T24555	6295-6299	NN	denotes	p3xF
T24556	6299-6304	RB	denotes	LAG-T
T24557	6304-6305	-COMMA-	denotes	a
T24558	6305-6310	NN	denotes	t (5
T24559	6310-6314	VB	denotes	µg);
T24560	6315-6325	VB	denotes	48 h later
T24561	6325-6329	IN	denotes	, ce
T24562	6329-6332	NN	denotes	lls
T24563	6333-6334	-LRB-	denotes	w
T24564	6334-6339	NN	denotes	ere s
T24565	6339-6340	-COMMA-	denotes	t
T24566	6340-6348	NN	denotes	imulated
T24567	6349-6350	-RRB-	denotes	w
T24568	6350-6352	CC	denotes	it
T24569	6352-6356	VB	denotes	h PM
T24570	6356-6368	JJ	denotes	A (5 min, 20
T24571	6368-6369	-COMMA-	denotes
T24572	6369-6375	VB	denotes	ng/ml)
T24573	6376-6381	RB	denotes	or le
T24574	6381-6385	IN	denotes	ft u
T24575	6385-6389	NN	denotes	nsti
T24576	6389-6390	-COMMA-	denotes	m
T24577	6390-6393	CC	denotes	ula
T24578	6393-6402	VB	denotes	ted, wash
T24579	6402-6407	IN	denotes	ed tw
T24580	6407-6414	NN	denotes	ice wit
T24581	6451-6456	JJ	denotes	Whole
T24582	6457-6461	NN	denotes	cell
T24583	6462-6470	NN	denotes	extracts
T24584	6471-6472	-LRB-	denotes	(
T24585	6472-6476	NN	denotes	1 mg
T24586	6476-6477	-RRB-	denotes	)
T24587	6478-6482	VB	denotes	were
T24588	6483-6501	VB	denotes	immunoprecipitated
T24589	6502-6506	IN	denotes	with
T24590	6507-6514	NN	denotes	protein
T24591	6515-6534	JJ	denotes	G-Sepharose-coupled
T24592	6535-6545	JJ	denotes	anti-IκB-α
T24593	6546-6554	NN	denotes	antibody
T24594	6556-6571	NN	denotes	Immunocomplexes
T24595	6572-6576	VB	denotes	were
T24596	6577-6586	VB	denotes	separated
T24597	6587-6589	IN	denotes	by
T24598	6590-6592	CD	denotes	12
T24599	6592-6593	NN	denotes	%
T24600	6594-6602	NN	denotes	SDS–PAGE
T24601	6603-6606	CC	denotes	and
T24602	6607-6615	VB	denotes	analysed
T24603	6616-6618	IN	denotes	by
T24604	6619-6626	JJ	denotes	western
T24605	6627-6635	NN	denotes	blotting
T24606	6636-6640	IN	denotes	with
T24607	6641-6649	JJ	denotes	anti-p65
T24608	6649-6650	-COMMA-	denotes	,
T24609	6651-6660	JJ	denotes	anti-FLAG
T24610	6661-6664	CC	denotes	and
T24611	6665-6675	JJ	denotes	anti-IκB-α
T24612	6676-6686	NN	denotes	antibodies
T24613	6688-6700	NN	denotes	Densitometry
T24614	6701-6707	NN	denotes	values
T24615	6708-6709	-LRB-	denotes	(
T24616	6709-6710	NN	denotes	D
T24617	6710-6711	-RRB-	denotes	)
T24618	6712-6714	IN	denotes	of
T24619	6715-6718	DT	denotes	the
T24620	6719-6724	NN	denotes	bands
T24621	6725-6729	VB	denotes	were
T24622	6730-6739	VB	denotes	expressed
T24623	6740-6742	IN	denotes	as
T24624	6743-6747	JJ	denotes	fold
T24625	6748-6756	NN	denotes	increase
T24626	6757-6762	IN	denotes	above
T24627	6763-6766	DT	denotes	the
T24628	6767-6774	NN	denotes	control
T24629	6775-6776	-LRB-	denotes	(
T24630	6776-6780	NN	denotes	lane
T24631	6781-6782	CD	denotes	1
T24632	6782-6783	-RRB-	denotes	)
T24633	6785-6791	NN	denotes	Values
T24634	6792-6793	-LRB-	denotes	(
T24635	6793-6802	NN	denotes	mean ± SE
T24636	6802-6803	-COMMA-	denotes	,
T24637	6804-6809	NN	denotes	n = 3
T24638	6809-6810	-RRB-	denotes	)
T24639	6811-6814	VB	denotes	are
T24640	6815-6820	VB	denotes	shown
R7042	T8554	T8555	arg1Of	Tat,enhances
R7043	T8554	T8560	arg1Of	Tat,hijacking
R7044	T8554	T8563	arg1Of	Tat,inhibiting
R7045	T8555	T8559	arg1Of	enhances,by
R7046	T8558	T8555	arg2Of	activity,enhances
R7047	T8558	T8556	arg1Of	activity,the
R7048	T8558	T8557	arg1Of	activity,NF-κB
R7049	T8560	T8562	arg1Of	hijacking,and
R7050	T8561	T8560	arg2Of	IκB-α,hijacking
R7051	T8562	T8559	arg2Of	and,by
R7052	T8563	T8562	arg2Of	inhibiting,and
R7053	T8563	T8567	arg1Of	inhibiting,off
R7054	T8566	T8563	arg2Of	turn,inhibiting
R7055	T8566	T8564	arg1Of	turn,the
R7056	T8566	T8565	arg1Of	turn,post-activation
R7057	T8567	T8568	arg1Of	off,of
R7058	T8569	T8568	arg2Of	NF-κB,of
R7059	T8570	T8571	arg1Of	We,analysed
R7060	T8570	T8583	arg1Of	We,modulating
R7061	T8571	T8582	arg1Of	analysed,by
R7062	T8573	T8571	arg2Of	kinetic,analysed
R7063	T8573	T8572	arg1Of	kinetic,the
R7064	T8573	T8574	arg1Of	kinetic,of
R7065	T8576	T8574	arg2Of	activation,of
R7066	T8576	T8575	arg1Of	activation,NF-κB
R7067	T8576	T8577	arg1Of	activation,in
R7068	T8581	T8577	arg2Of	infection,in
R7069	T8581	T8578	arg1Of	infection,single
R7070	T8581	T8579	arg1Of	infection,round
R7071	T8581	T8580	arg1Of	infection,HIV-1
R7072	T8583	T8582	arg2Of	modulating,by
R7073	T8585	T8583	arg2Of	expression,modulating
R7074	T8585	T8584	arg1Of	expression,the
R7075	T8585	T8586	arg1Of	expression,of
R7076	T8587	T8586	arg2Of	Tat,of
R7077	T8587	T8588	arg1Of	Tat,with
R7078	T8590	T8588	arg2Of	interference,with
R7079	T8590	T8589	arg1Of	interference,RNA
R7080	T8592	T8591	arg1Of	cells,Jurkat
R7081	T8592	T8593	arg1Of	cells,were
R7082	T8592	T8594	arg2Of	cells,transfected
R7083	T8592	T8603	arg1Of	cells,left
R7084	T8594	T8593	arg2Of	transfected,were
R7085	T8594	T8595	arg1Of	transfected,with
R7086	T8594	T8602	arg1Of	transfected,or
R7087	T8597	T8596	arg1Of	Tat,siRNA
R7088	T8597	T8598	arg1Of	Tat,","
R7089	T8598	T8595	arg2Of	",",with
R7090	T8600	T8598	arg2Of	control,","
R7091	T8600	T8599	arg1Of	control,siRNA
R7092	T8602	T8601	arg1Of	or,","
R7093	T8602	T8606	arg1Of	or,and
R7094	T8603	T8602	arg2Of	left,or
R7095	T8604	T8603	arg2Of	untransfected,left
R7096	T8606	T8605	arg1Of	and,","
R7097	T8609	T8607	arg1Of	cells,24 h
R7098	T8609	T8608	arg1Of	cells,later
R7099	T8609	T8610	arg1Of	cells,were
R7100	T8609	T8611	arg2Of	cells,infected
R7101	T8611	T8606	arg2Of	infected,and
R7102	T8611	T8610	arg2Of	infected,were
R7103	T8611	T8612	arg1Of	infected,with
R7104	T8616	T8612	arg2Of	virions,with
R7105	T8616	T8613	arg1Of	virions,HXB2
R7106	T8616	T8614	arg1Of	virions,Env-pseudotyped
R7107	T8616	T8615	arg1Of	virions,NL4-3.Luc.R−E−
R7108	T8619	T8617	arg2Of	analysis,By
R7109	T8619	T8618	arg1Of	analysis,cytofluorimetric
R7110	T8622	T8621	arg1Of	%,∼40
R7111	T8622	T8623	arg1Of	%,of
R7112	T8622	T8625	arg1Of	%,was
R7113	T8622	T8626	arg1Of	%,siRNA-transfected
R7114	T8622	T8628	arg1Of	%,HIV-1-infected
R7115	T8624	T8623	arg2Of	cells,of
R7116	T8625	T8617	arg1Of	was,By
R7117	T8625	T8620	arg1Of	was,","
R7118	T8625	T8629	arg1Of	was,at
R7119	T8626	T8627	arg1Of	siRNA-transfected,and
R7120	T8627	T8625	arg2Of	and,was
R7121	T8628	T8627	arg2Of	HIV-1-infected,and
R7122	T8631	T8629	arg2Of	post-infection,at
R7123	T8631	T8630	arg1Of	post-infection,12-h
R7124	T8631	T8632	arg1Of	post-infection,(
R7125	T8635	T8632	arg2Of	S1,(
R7126	T8635	T8633	arg1Of	S1,Supplementary
R7127	T8635	T8634	arg1Of	S1,Figure
R7128	T8636	T8632	arg3Of	),(
R7129	T8638	T8637	arg1Of	expression,The
R7130	T8638	T8639	arg1Of	expression,of
R7131	T8638	T8641	arg1Of	expression,was
R7132	T8638	T8642	arg2Of	expression,detected
R7133	T8638	T8648	arg1Of	expression,increased
R7134	T8638	T8651	arg1Of	expression,12 h
R7135	T8640	T8639	arg2Of	Tat,of
R7136	T8642	T8641	arg2Of	detected,was
R7137	T8642	T8643	arg1Of	detected,at
R7138	T8642	T8646	arg1Of	detected,and
R7139	T8645	T8643	arg2Of	post-infection,at
R7140	T8645	T8644	arg1Of	post-infection,1-h
R7141	T8648	T8646	arg2Of	increased,and
R7142	T8648	T8647	arg1Of	increased,progressively
R7143	T8648	T8649	arg1Of	increased,up
R7144	T8648	T8650	modOf	increased,to
R7145	T8648	T8658	arg1Of	increased,","
R7146	T8648	T8659	arg1Of	increased,while
R7147	T8651	T8650	arg1Of	12 h,to
R7148	T8651	T8652	arg1Of	12 h,in
R7149	T8653	T8654	arg1Of	untransfected,and
R7150	T8656	T8654	arg2Of	control-transfected,and
R7151	T8656	T8655	arg1Of	control-transfected,siRNA
R7152	T8657	T8652	arg2Of	cells,in
R7153	T8657	T8653	arg1Of	cells,untransfected
R7154	T8657	T8656	arg1Of	cells,control-transfected
R7155	T8660	T8661	arg1Of	it,was
R7156	T8660	T8663	arg2Of	it,detected
R7157	T8663	T8659	arg2Of	detected,while
R7158	T8663	T8661	arg2Of	detected,was
R7159	T8663	T8662	arg1Of	detected,barely
R7160	T8663	T8664	arg1Of	detected,in
R7161	T8667	T8664	arg2Of	cells,in
R7162	T8667	T8665	arg1Of	cells,siRNA
R7163	T8667	T8666	arg1Of	cells,Tat-transfected
R7164	T8667	T8668	arg1Of	cells,(
R7165	T8670	T8668	arg2Of	1A,(
R7166	T8670	T8669	arg1Of	1A,Figure
R7167	T8671	T8668	arg3Of	),(
R7168	T8675	T8672	arg1Of	expression,The
R7169	T8675	T8673	arg1Of	expression,LTR-dependent
R7170	T8675	T8674	arg1Of	expression,luciferase
R7171	T8675	T8676	arg1Of	expression,was
R7172	T8675	T8679	arg2Of	expression,induced
R7173	T8679	T8676	arg2Of	induced,was
R7174	T8679	T8677	arg1Of	induced,also
R7175	T8679	T8678	arg1Of	induced,progressively
R7176	T8679	T8680	arg1Of	induced,at
R7177	T8679	T8685	arg1Of	induced,in
R7178	T8679	T8691	arg1Of	induced,","
R7179	T8679	T8692	arg1Of	induced,while
R7180	T8683	T8681	arg1Of	12-h,1-
R7181	T8683	T8682	arg1Of	12-h,to
R7182	T8684	T8680	arg2Of	post-infection,at
R7183	T8684	T8683	arg1Of	post-infection,12-h
R7184	T8686	T8687	arg1Of	untransfected,and
R7185	T8689	T8687	arg2Of	control-transfected,and
R7186	T8689	T8688	arg1Of	control-transfected,siRNA
R7187	T8690	T8685	arg2Of	cells,in
R7188	T8690	T8686	arg1Of	cells,untransfected
R7189	T8690	T8689	arg1Of	cells,control-transfected
R7190	T8693	T8694	arg1Of	it,was
R7191	T8693	T8696	arg2Of	it,observed
R7192	T8696	T8692	arg2Of	observed,while
R7193	T8696	T8694	arg2Of	observed,was
R7194	T8696	T8695	arg1Of	observed,barely
R7195	T8696	T8697	arg1Of	observed,in
R7196	T8696	T8701	arg1Of	observed,","
R7197	T8700	T8697	arg2Of	cells,in
R7198	T8700	T8698	arg1Of	cells,siRNA
R7199	T8700	T8699	arg1Of	cells,Tat-transfected
R7200	T8702	T8696	arg3Of	as,observed
R7201	T8703	T8702	arg2Of	expected,as
R7202	T8703	T8704	arg1Of	expected,for
R7203	T8707	T8704	arg2Of	transactivation,for
R7204	T8707	T8705	arg1Of	transactivation,the
R7205	T8707	T8706	arg1Of	transactivation,Tat-dependent
R7206	T8707	T8708	arg1Of	transactivation,of
R7207	T8711	T8708	arg2Of	LTR,of
R7208	T8711	T8709	arg1Of	LTR,the
R7209	T8711	T8710	arg1Of	LTR,HIV-1
R7210	T8711	T8712	arg1Of	LTR,(
R7211	T8714	T8712	arg2Of	1A,(
R7212	T8714	T8713	arg1Of	1A,Figure
R7213	T8715	T8712	arg3Of	),(
R7214	T8718	T8716	arg1Of	increase,The
R7215	T8718	T8717	arg1Of	increase,progressive
R7216	T8718	T8719	arg1Of	increase,in
R7217	T8718	T8747	arg1Of	increase,was
R7218	T8718	T8748	arg2Of	increase,observed
R7219	T8721	T8719	arg2Of	activity,in
R7220	T8721	T8720	arg1Of	activity,NF-κB
R7221	T8724	T8723	arg2Of	measured,as
R7222	T8727	T8724	arg1Of	binding,measured
R7223	T8727	T8725	arg2Of	binding,by
R7224	T8727	T8726	arg1Of	binding,p65–p50
R7225	T8727	T8728	arg1Of	binding,to
R7226	T8732	T8729	arg1Of	oligonucleotide,an
R7227	T8732	T8730	arg1Of	oligonucleotide,NF-κB
R7228	T8732	T8731	arg1Of	oligonucleotide,consensus
R7229	T8732	T8733	arg1Of	oligonucleotide,(
R7230	T8732	T8737	arg1Of	oligonucleotide,and
R7231	T8735	T8733	arg2Of	1B,(
R7232	T8735	T8734	arg1Of	1B,Figure
R7233	T8736	T8733	arg3Of	),(
R7234	T8737	T8728	arg2Of	and,to
R7235	T8739	T8737	arg2Of	p65,and
R7236	T8739	T8738	arg1Of	p65,nuclear
R7237	T8739	T8740	arg1Of	p65,(
R7238	T8742	T8740	arg2Of	1C,(
R7239	T8742	T8741	arg1Of	1C,Figure
R7240	T8742	T8743	arg1Of	1C,","
R7241	T8744	T8743	arg2Of	nucleus,","
R7242	T8745	T8740	arg3Of	),(
R7243	T8748	T8722	arg1Of	observed,","
R7244	T8748	T8723	arg1Of	observed,as
R7245	T8748	T8746	arg1Of	observed,","
R7246	T8748	T8747	arg2Of	observed,was
R7247	T8748	T8749	arg1Of	observed,at
R7248	T8748	T8754	arg1Of	observed,in
R7249	T8748	T8771	arg1Of	observed,;
R7250	T8752	T8750	arg1Of	3-h,1-
R7251	T8752	T8751	arg1Of	3-h,to
R7252	T8753	T8749	arg2Of	post-infection,at
R7253	T8753	T8752	arg1Of	post-infection,3-h
R7254	T8756	T8755	arg1Of	Tat-positive,both
R7255	T8756	T8757	arg1Of	Tat-positive,(
R7256	T8756	T8764	arg1Of	Tat-positive,and
R7257	T8759	T8760	arg1Of	siRNA,or
R7258	T8760	T8757	arg2Of	or,(
R7259	T8760	T8758	arg1Of	or,no
R7260	T8762	T8760	arg2Of	control,or
R7261	T8762	T8761	arg1Of	control,siRNA
R7262	T8763	T8757	arg3Of	),(
R7263	T8764	T8754	arg2Of	and,in
R7264	T8768	T8766	arg2Of	Tat,(
R7265	T8768	T8767	arg1Of	Tat,siRNA
R7266	T8769	T8766	arg3Of	),(
R7267	T8770	T8764	arg2Of	cells,and
R7268	T8770	T8765	arg1Of	cells,Tat-negative
R7269	T8770	T8766	arg1Of	cells,(
R7270	T8771	T8772	arg1Of	;,however
R7271	T8771	T8773	arg1Of	;,","
R7272	T8776	T8774	arg2Of	post-infection,at
R7273	T8776	T8775	arg1Of	post-infection,12-h
R7274	T8781	T8779	arg1Of	binding,p65
R7275	T8781	T8780	arg1Of	binding,DNA
R7276	T8781	T8782	arg1Of	binding,and
R7277	T8782	T8778	arg1Of	and,the
R7278	T8782	T8785	arg1Of	and,persisted
R7279	T8782	T8786	arg2Of	and,elevated
R7280	T8784	T8782	arg2Of	p65,and
R7281	T8784	T8783	arg1Of	p65,nuclear
R7282	T8785	T8771	arg2Of	persisted,;
R7283	T8785	T8774	arg1Of	persisted,at
R7284	T8785	T8777	arg1Of	persisted,","
R7285	T8785	T8786	modOf	persisted,elevated
R7286	T8785	T8798	arg1Of	persisted,","
R7287	T8785	T8799	arg1Of	persisted,while
R7288	T8786	T8787	arg1Of	elevated,in
R7289	T8787	T8789	arg1Of	in,of
R7290	T8788	T8787	arg2Of	presence,in
R7291	T8790	T8787	arg3Of	Tat,in
R7292	T8790	T8791	arg1Of	Tat,(
R7293	T8793	T8794	arg1Of	siRNA,and
R7294	T8794	T8791	arg2Of	and,(
R7295	T8794	T8792	arg1Of	and,no
R7296	T8796	T8794	arg2Of	control,and
R7297	T8796	T8795	arg1Of	control,siRNA
R7298	T8797	T8791	arg3Of	),(
R7299	T8800	T8801	arg1Of	they,dropped
R7300	T8801	T8799	arg2Of	dropped,while
R7301	T8801	T8802	arg1Of	dropped,in
R7302	T8802	T8804	arg1Of	in,of
R7303	T8803	T8802	arg2Of	absence,in
R7304	T8805	T8802	arg3Of	Tat,in
R7305	T8805	T8806	arg1Of	Tat,(
R7306	T8805	T8810	arg1Of	Tat,(
R7307	T8808	T8806	arg2Of	Tat,(
R7308	T8808	T8807	arg1Of	Tat,siRNA
R7309	T8809	T8806	arg3Of	),(
R7310	T8812	T8811	arg1Of	1B,Figure
R7311	T8812	T8813	arg1Of	1B,and
R7312	T8813	T8810	arg2Of	and,(
R7313	T8814	T8813	arg2Of	C,and
R7314	T8815	T8810	arg3Of	),(
R7315	T8816	T8817	arg1Of	IκB-α,was
R7316	T8816	T8818	arg2Of	IκB-α,degraded
R7317	T8816	T8828	arg2Of	IκB-α,synthesized
R7318	T8818	T8817	arg2Of	degraded,was
R7319	T8818	T8819	arg1Of	degraded,in
R7320	T8818	T8827	arg1Of	degraded,novo
R7321	T8818	T8828	modOf	degraded,synthesized
R7322	T8821	T8819	arg2Of	cytosol,in
R7323	T8821	T8820	arg1Of	cytosol,the
R7324	T8821	T8822	arg1Of	cytosol,within
R7325	T8824	T8822	arg2Of	post-infection,within
R7326	T8824	T8823	arg1Of	post-infection,3-h
R7327	T8827	T8825	arg1Of	novo,and
R7328	T8827	T8826	arg1Of	novo,de
R7329	T8828	T8829	arg1Of	synthesized,at
R7330	T8828	T8834	arg1Of	synthesized,at
R7331	T8830	T8829	arg2Of	6 h,at
R7332	T8830	T8831	arg1Of	6 h,with
R7333	T8833	T8831	arg2Of	replenishment,with
R7334	T8833	T8832	arg1Of	replenishment,full
R7335	T8836	T8834	arg2Of	post-infection,at
R7336	T8836	T8835	arg1Of	post-infection,12-h
R7337	T8836	T8837	arg1Of	post-infection,in
R7338	T8839	T8840	arg1Of	Tat-positive,and
R7339	T8841	T8840	arg2Of	Tat-negative,and
R7340	T8842	T8837	arg2Of	cells,in
R7341	T8842	T8838	arg1Of	cells,both
R7342	T8842	T8839	arg1Of	cells,Tat-positive
R7343	T8842	T8841	arg1Of	cells,Tat-negative
R7344	T8842	T8843	arg1Of	cells,(
R7345	T8845	T8843	arg2Of	1C,(
R7346	T8845	T8844	arg1Of	1C,Figure
R7347	T8845	T8846	arg1Of	1C,","
R7348	T8847	T8846	arg2Of	cytosol,","
R7349	T8848	T8843	arg3Of	),(
R7350	T8853	T8851	arg1Of	activity,the
R7351	T8853	T8852	arg1Of	activity,IKK
R7352	T8853	T8854	arg1Of	activity,was
R7353	T8853	T8855	arg2Of	activity,induced
R7354	T8853	T8861	arg1Of	activity,decreased
R7355	T8855	T8854	arg2Of	induced,was
R7356	T8855	T8856	arg1Of	induced,at
R7357	T8855	T8860	arg1Of	induced,and
R7358	T8858	T8856	arg2Of	post-infection,at
R7359	T8858	T8857	arg1Of	post-infection,1-h
R7360	T8860	T8849	arg1Of	and,Consistently
R7361	T8860	T8850	arg1Of	and,","
R7362	T8860	T8859	arg1Of	and,","
R7363	T8861	T8860	arg2Of	decreased,and
R7364	T8861	T8862	arg1Of	decreased,at
R7365	T8862	T8864	arg1Of	at,post-infection
R7366	T8863	T8862	arg2Of	3-h,at
R7367	T8864	T8865	arg1Of	post-infection,independently
R7368	T8865	T8866	arg1Of	independently,of
R7369	T8868	T8866	arg2Of	presence,of
R7370	T8868	T8867	arg1Of	presence,the
R7371	T8868	T8869	arg1Of	presence,of
R7372	T8870	T8869	arg2Of	Tat,of
R7373	T8870	T8871	arg1Of	Tat,(
R7374	T8873	T8871	arg2Of	1D,(
R7375	T8873	T8872	arg1Of	1D,Figure
R7376	T8874	T8871	arg3Of	),(
R7377	T8876	T8875	arg1Of	kinetics,Similar
R7378	T8876	T8877	arg1Of	kinetics,of
R7379	T8876	T8886	arg1Of	kinetics,were
R7380	T8876	T8887	arg2Of	kinetics,observed
R7381	T8879	T8878	arg1Of	activity,NF-κB
R7382	T8879	T8880	arg1Of	activity,","
R7383	T8880	T8883	arg1Of	",",and
R7384	T8882	T8880	arg2Of	activation,","
R7385	T8882	T8881	arg1Of	activation,IKK
R7386	T8883	T8877	arg2Of	and,of
R7387	T8885	T8883	arg2Of	degradation/re-synthesis,and
R7388	T8885	T8884	arg1Of	degradation/re-synthesis,IκB-α
R7389	T8887	T8886	arg2Of	observed,were
R7390	T8887	T8888	arg1Of	observed,in
R7391	T8891	T8888	arg2Of	infection,in
R7392	T8891	T8889	arg1Of	infection,single-round
R7393	T8891	T8890	arg1Of	infection,HIV-1
R7394	T8891	T8892	arg1Of	infection,of
R7395	T8893	T8892	arg2Of	PBMCs,of
R7396	T8893	T8894	arg1Of	PBMCs,using
R7397	T8894	T8907	arg1Of	using,as
R7398	T8896	T8894	arg2Of	cocktail,using
R7399	T8896	T8895	arg1Of	cocktail,a
R7400	T8896	T8897	arg1Of	cocktail,of
R7401	T8898	T8899	arg1Of	Azidothymidine,(
R7402	T8898	T8902	arg1Of	Azidothymidine,and
R7403	T8900	T8899	arg2Of	AZT,(
R7404	T8901	T8899	arg3Of	),(
R7405	T8902	T8897	arg2Of	and,of
R7406	T8903	T8902	arg2Of	Lamivudine,and
R7407	T8903	T8904	arg1Of	Lamivudine,(
R7408	T8905	T8904	arg2Of	3TC,(
R7409	T8906	T8904	arg3Of	),(
R7410	T8910	T8907	arg2Of	inhibitors,as
R7411	T8910	T8908	arg1Of	inhibitors,reverse
R7412	T8910	T8909	arg1Of	inhibitors,transcriptase
R7413	T8910	T8911	arg1Of	inhibitors,(
R7414	T8914	T8911	arg2Of	S2,(
R7415	T8914	T8912	arg1Of	S2,Supplementary
R7416	T8914	T8913	arg1Of	S2,Figure
R7417	T8915	T8911	arg3Of	),(
R7418	T8917	T8916	arg1Of	results,These
R7419	T8917	T8918	arg1Of	results,indicated
R7420	T8923	T8920	arg2Of	infection,in
R7421	T8923	T8921	arg1Of	infection,single-round
R7422	T8923	T8922	arg1Of	infection,HIV-1
R7423	T8926	T8924	arg1Of	activation,the
R7424	T8926	T8925	arg1Of	activation,NF-κB
R7425	T8926	T8928	arg1Of	activation,correlated
R7426	T8928	T8927	arg1Of	correlated,initially
R7427	T8928	T8929	arg1Of	correlated,with
R7428	T8928	T8936	arg1Of	correlated,independently
R7429	T8928	T8943	arg1Of	correlated,and
R7430	T8932	T8931	arg1Of	activation,IKK
R7431	T8932	T8933	arg1Of	activation,and
R7432	T8933	T8929	arg2Of	and,with
R7433	T8933	T8930	arg1Of	and,the
R7434	T8935	T8933	arg2Of	degradation,and
R7435	T8935	T8934	arg1Of	degradation,IκB-α
R7436	T8936	T8937	arg1Of	independently,of
R7437	T8939	T8937	arg2Of	presence,of
R7438	T8939	T8938	arg1Of	presence,the
R7439	T8939	T8940	arg1Of	presence,of
R7440	T8941	T8940	arg2Of	Tat,of
R7441	T8943	T8918	arg2Of	and,indicated
R7442	T8943	T8919	arg1Of	and,that
R7443	T8943	T8920	arg1Of	and,in
R7444	T8943	T8942	arg1Of	and,","
R7445	T8944	T8945	arg1Of	it,was
R7446	T8944	T8946	arg2Of	it,kept
R7447	T8944	T8947	arg2Of	it,elevated
R7448	T8946	T8943	arg2Of	kept,and
R7449	T8946	T8945	arg2Of	kept,was
R7450	T8947	T8946	arg3Of	elevated,kept
R7451	T8947	T8948	arg1Of	elevated,in
R7452	T8949	T8950	arg1Of	presence,of
R7453	T8949	T8952	arg1Of	presence,following
R7454	T8949	T8959	arg1Of	presence,and
R7455	T8951	T8950	arg2Of	Tat,of
R7456	T8954	T8952	arg2Of	decay,following
R7457	T8954	T8953	arg1Of	decay,the
R7458	T8954	T8955	arg1Of	decay,of
R7459	T8958	T8955	arg2Of	activity,of
R7460	T8958	T8956	arg1Of	activity,the
R7461	T8958	T8957	arg1Of	activity,IKK
R7462	T8959	T8948	arg2Of	and,in
R7463	T8961	T8959	arg2Of	synthesis,and
R7464	T8961	T8960	arg1Of	synthesis,new
R7465	T8961	T8962	arg1Of	synthesis,of
R7466	T8963	T8962	arg2Of	IκB-α,of
R7467	T8966	T8967	arg1Of	we,tested
R7468	T8967	T8964	arg1Of	tested,Next
R7469	T8967	T8965	arg1Of	tested,","
R7470	T8970	T8967	arg2Of	action,tested
R7471	T8970	T8968	arg1Of	action,the
R7472	T8970	T8969	arg1Of	action,single
R7473	T8970	T8971	arg1Of	action,of
R7474	T8970	T8973	arg1Of	action,on
R7475	T8972	T8971	arg2Of	Tat,of
R7476	T8975	T8973	arg2Of	kinetic,on
R7477	T8975	T8974	arg1Of	kinetic,the
R7478	T8975	T8976	arg1Of	kinetic,of
R7479	T8978	T8976	arg2Of	activity,of
R7480	T8978	T8977	arg1Of	activity,NF-κB
R7481	T8978	T8979	arg2Of	activity,induced
R7482	T8981	T8979	arg1Of	PMA,induced
R7483	T8981	T8980	arg2Of	PMA,by
R7484	T8984	T8982	arg2Of	end,To
R7485	T8984	T8983	arg1Of	end,this
R7486	T8987	T8986	arg1Of	cells,HeLa
R7487	T8987	T8988	arg1Of	cells,were
R7488	T8987	T8989	arg2Of	cells,transfected
R7489	T8989	T8982	arg1Of	transfected,To
R7490	T8989	T8985	arg1Of	transfected,","
R7491	T8989	T8988	arg2Of	transfected,were
R7492	T8989	T8990	arg1Of	transfected,with
R7493	T8991	T8993	arg1Of	p3x-FLAG-Tat,or
R7494	T8993	T8990	arg2Of	or,with
R7495	T8993	T8992	arg1Of	or,","
R7496	T8993	T8996	arg1Of	or,","
R7497	T8993	T8997	arg2Of	or,stimulated
R7498	T8993	T9005	arg2Of	or,washed
R7499	T8993	T9007	arg1Of	or,avoid
R7500	T8995	T8993	arg2Of	vector,or
R7501	T8995	T8994	arg1Of	vector,empty
R7502	T8997	T8998	arg1Of	stimulated,with
R7503	T8997	T9003	arg1Of	stimulated,and
R7504	T8999	T8998	arg2Of	PMA,with
R7505	T8999	T9000	arg1Of	PMA,for
R7506	T9001	T9000	arg2Of	5 min,for
R7507	T9003	T9002	arg1Of	and,","
R7508	T9005	T9003	arg2Of	washed,and
R7509	T9005	T9004	arg1Of	washed,extensively
R7510	T9007	T9005	arg3Of	avoid,washed
R7511	T9007	T9006	arg1Of	avoid,to
R7512	T9010	T9007	arg2Of	kinetic,avoid
R7513	T9010	T9008	arg1Of	kinetic,the
R7514	T9010	T9009	arg1Of	kinetic,oscillatory
R7515	T9010	T9011	arg1Of	kinetic,of
R7516	T9013	T9011	arg2Of	activation,of
R7517	T9013	T9012	arg1Of	activation,NF-κB
R7518	T9013	T9014	arg1Of	activation,(
R7519	T9015	T9014	arg2Of	"17,18",(
R7520	T9016	T9014	arg3Of	),(
R7521	T9019	T9017	arg2Of	cells,In
R7522	T9019	T9018	arg1Of	cells,un-stimulated
R7523	T9024	T9021	arg1Of	binding,the
R7524	T9024	T9022	arg1Of	binding,p65
R7525	T9024	T9023	arg1Of	binding,DNA
R7526	T9024	T9025	arg1Of	binding,(
R7527	T9024	T9029	arg1Of	binding,and
R7528	T9027	T9025	arg2Of	2A,(
R7529	T9027	T9026	arg1Of	2A,Figure
R7530	T9028	T9025	arg3Of	),(
R7531	T9029	T9038	arg1Of	and,were
R7532	T9029	T9040	arg2Of	and,enhanced
R7533	T9031	T9029	arg2Of	p65,and
R7534	T9031	T9030	arg1Of	p65,nuclear
R7535	T9031	T9032	arg1Of	p65,(
R7536	T9034	T9032	arg2Of	2B,(
R7537	T9034	T9033	arg1Of	2B,Figure
R7538	T9034	T9035	arg1Of	2B,","
R7539	T9036	T9035	arg2Of	nucleus,","
R7540	T9037	T9032	arg3Of	),(
R7541	T9040	T9017	arg1Of	enhanced,In
R7542	T9040	T9020	arg1Of	enhanced,","
R7543	T9040	T9038	arg2Of	enhanced,were
R7544	T9040	T9039	arg1Of	enhanced,slightly
R7545	T9040	T9043	arg1Of	enhanced,","
R7546	T9040	T9044	arg1Of	enhanced,while
R7547	T9040	T9052	arg1Of	enhanced,(
R7548	T9042	T9040	arg1Of	Tat,enhanced
R7549	T9042	T9041	arg2Of	Tat,by
R7550	T9047	T9045	arg1Of	content,the
R7551	T9047	T9046	arg1Of	content,IκB-α
R7552	T9047	T9048	arg1Of	content,was
R7553	T9047	T9051	arg2Of	content,affected
R7554	T9051	T9044	arg2Of	affected,while
R7555	T9051	T9048	arg2Of	affected,was
R7556	T9051	T9049	arg1Of	affected,not
R7557	T9051	T9050	arg1Of	affected,significantly
R7558	T9054	T9052	arg2Of	2B,(
R7559	T9054	T9053	arg1Of	2B,Figure
R7560	T9054	T9055	arg1Of	2B,","
R7561	T9056	T9055	arg2Of	cytosol,","
R7562	T9057	T9052	arg3Of	),(
R7563	T9059	T9058	arg1Of	stimulation,Transient
R7564	T9059	T9060	arg1Of	stimulation,with
R7565	T9059	T9062	arg1Of	stimulation,increased
R7566	T9061	T9060	arg2Of	PMA,with
R7567	T9062	T9076	arg1Of	increased,independently
R7568	T9062	T9088	arg1Of	increased,;
R7569	T9067	T9064	arg1Of	activity,NF-κB
R7570	T9067	T9065	arg1Of	activity,DNA
R7571	T9067	T9066	arg1Of	activity,binding
R7572	T9067	T9068	arg1Of	activity,and
R7573	T9068	T9062	arg2Of	and,increased
R7574	T9068	T9063	arg1Of	and,the
R7575	T9068	T9071	arg1Of	and,at
R7576	T9070	T9068	arg2Of	p65,and
R7577	T9070	T9069	arg1Of	p65,nuclear
R7578	T9072	T9071	arg2Of	5 min,at
R7579	T9072	T9073	arg1Of	5 min,peaking
R7580	T9073	T9074	arg1Of	peaking,at
R7581	T9075	T9074	arg2Of	60–120 min,at
R7582	T9076	T9077	arg1Of	independently,of
R7583	T9079	T9077	arg2Of	presence,of
R7584	T9079	T9078	arg1Of	presence,the
R7585	T9079	T9080	arg1Of	presence,of
R7586	T9081	T9080	arg2Of	Tat,of
R7587	T9081	T9082	arg1Of	Tat,(
R7588	T9084	T9083	arg1Of	2A,Figure
R7589	T9084	T9085	arg1Of	2A,and
R7590	T9085	T9082	arg2Of	and,(
R7591	T9086	T9085	arg2Of	B,and
R7592	T9087	T9082	arg3Of	),(
R7593	T9095	T9093	arg1Of	binding,p65
R7594	T9095	T9094	arg1Of	binding,DNA
R7595	T9095	T9096	arg1Of	binding,and
R7596	T9096	T9092	arg1Of	and,the
R7597	T9096	T9099	arg1Of	and,persisted
R7598	T9096	T9100	arg2Of	and,elevated
R7599	T9098	T9096	arg2Of	p65,and
R7600	T9098	T9097	arg1Of	p65,nuclear
R7601	T9099	T9091	arg2Of	persisted,while
R7602	T9099	T9100	modOf	persisted,elevated
R7603	T9099	T9101	arg1Of	persisted,at
R7604	T9099	T9104	arg1Of	persisted,in
R7605	T9103	T9101	arg2Of	post-treatment,at
R7606	T9103	T9102	arg1Of	post-treatment,240-min
R7607	T9106	T9104	arg2Of	cells,in
R7608	T9106	T9105	arg1Of	cells,Tat-positive
R7609	T9108	T9109	arg1Of	they,dropped
R7610	T9109	T9088	arg2Of	dropped,;
R7611	T9109	T9089	arg1Of	dropped,however
R7612	T9109	T9090	arg1Of	dropped,","
R7613	T9109	T9091	arg1Of	dropped,while
R7614	T9109	T9107	arg1Of	dropped,","
R7615	T9109	T9110	arg1Of	dropped,in
R7616	T9112	T9110	arg2Of	cells,in
R7617	T9112	T9111	arg1Of	cells,Tat-negative
R7618	T9112	T9113	arg1Of	cells,(
R7619	T9115	T9114	arg1Of	2A,Figure
R7620	T9115	T9116	arg1Of	2A,and
R7621	T9116	T9113	arg2Of	and,(
R7622	T9117	T9116	arg2Of	B,and
R7623	T9118	T9113	arg3Of	),(
R7624	T9120	T9119	arg2Of	addition,In
R7625	T9122	T9123	arg1Of	Tat,bound
R7626	T9123	T9124	arg1Of	bound,to
R7627	T9123	T9131	arg1Of	bound,and
R7628	T9127	T9124	arg2Of	oligonucleotide,to
R7629	T9127	T9125	arg1Of	oligonucleotide,the
R7630	T9127	T9126	arg1Of	oligonucleotide,NF-κB
R7631	T9127	T9128	arg1Of	oligonucleotide,in
R7632	T9130	T9128	arg2Of	cells,in
R7633	T9130	T9129	arg1Of	cells,un-stimulated
R7634	T9131	T9119	arg1Of	and,In
R7635	T9131	T9121	arg1Of	and,","
R7636	T9133	T9132	arg1Of	binding,its
R7637	T9133	T9134	arg1Of	binding,increased
R7638	T9134	T9131	arg2Of	increased,and
R7639	T9134	T9135	arg1Of	increased,following
R7640	T9134	T9142	arg1Of	increased,","
R7641	T9137	T9135	arg2Of	stimulation,following
R7642	T9137	T9136	arg1Of	stimulation,PMA
R7643	T9137	T9138	arg1Of	stimulation,(
R7644	T9140	T9138	arg2Of	2A,(
R7645	T9140	T9139	arg1Of	2A,Figure
R7646	T9141	T9138	arg3Of	),(
R7647	T9143	T9134	arg2Of	suggesting,increased
R7648	T9143	T9156	arg1Of	suggesting,to
R7649	T9147	T9145	arg1Of	protein,the
R7650	T9147	T9146	arg1Of	protein,viral
R7651	T9147	T9148	arg1Of	protein,was
R7652	T9148	T9143	arg2Of	was,suggesting
R7653	T9148	T9144	arg1Of	was,that
R7654	T9150	T9148	arg2Of	component,was
R7655	T9150	T9149	arg1Of	component,a
R7656	T9150	T9151	arg1Of	component,of
R7657	T9154	T9151	arg2Of	complex,of
R7658	T9154	T9152	arg1Of	complex,the
R7659	T9154	T9153	arg1Of	complex,NF-κB
R7660	T9154	T9155	arg2Of	complex,bound
R7661	T9157	T9156	arg2Of	DNA,to
R7662	T9158	T9159	arg1Of	Degradation,of
R7663	T9158	T9161	arg1Of	Degradation,was
R7664	T9158	T9163	arg2Of	Degradation,induced
R7665	T9160	T9159	arg2Of	IκB-α,of
R7666	T9163	T9161	arg2Of	induced,was
R7667	T9163	T9162	arg1Of	induced,progressively
R7668	T9163	T9164	arg1Of	induced,at
R7669	T9163	T9166	arg1Of	induced,","
R7670	T9163	T9167	modOf	induced,followed
R7671	T9165	T9164	arg2Of	5–30 min,at
R7672	T9170	T9169	arg1Of	novo,de
R7673	T9171	T9167	arg1Of	synthesis,followed
R7674	T9171	T9168	arg2Of	synthesis,by
R7675	T9171	T9170	arg1Of	synthesis,novo
R7676	T9171	T9172	arg1Of	synthesis,at
R7677	T9173	T9172	arg2Of	60 min,at
R7678	T9173	T9174	arg1Of	60 min,with
R7679	T9176	T9174	arg2Of	replenishment,with
R7680	T9176	T9175	arg1Of	replenishment,full
R7681	T9176	T9177	arg1Of	replenishment,at
R7682	T9178	T9177	arg2Of	240 min,at
R7683	T9178	T9179	arg1Of	240 min,in
R7684	T9181	T9182	arg1Of	Tat-positive,and
R7685	T9183	T9182	arg2Of	negative,and
R7686	T9184	T9179	arg2Of	cells,in
R7687	T9184	T9180	arg1Of	cells,both
R7688	T9184	T9181	arg1Of	cells,Tat-positive
R7689	T9184	T9183	arg1Of	cells,negative
R7690	T9184	T9185	arg1Of	cells,(
R7691	T9187	T9185	arg2Of	2B,(
R7692	T9187	T9186	arg1Of	2B,Figure
R7693	T9187	T9188	arg1Of	2B,","
R7694	T9189	T9188	arg2Of	cytosol,","
R7695	T9190	T9185	arg3Of	),(
R7696	T9195	T9193	arg1Of	activation,the
R7697	T9195	T9194	arg1Of	activation,IKK
R7698	T9195	T9196	arg1Of	activation,started
R7699	T9195	T9201	arg1Of	activation,turned
R7700	T9196	T9197	arg1Of	started,at
R7701	T9196	T9200	arg1Of	started,and
R7702	T9198	T9197	arg2Of	5 min,at
R7703	T9200	T9191	arg1Of	and,Consistently
R7704	T9200	T9192	arg1Of	and,","
R7705	T9200	T9199	arg1Of	and,","
R7706	T9201	T9200	arg2Of	turned,and
R7707	T9201	T9202	arg1Of	turned,off
R7708	T9201	T9203	arg1Of	turned,at
R7709	T9201	T9205	arg1Of	turned,independently
R7710	T9204	T9203	arg2Of	30 min,at
R7711	T9205	T9206	arg1Of	independently,of
R7712	T9208	T9206	arg2Of	presence,of
R7713	T9208	T9207	arg1Of	presence,the
R7714	T9208	T9209	arg1Of	presence,of
R7715	T9210	T9209	arg2Of	Tat,of
R7716	T9210	T9211	arg1Of	Tat,(
R7717	T9213	T9211	arg2Of	2C,(
R7718	T9213	T9212	arg1Of	2C,Figure
R7719	T9214	T9211	arg3Of	),(
R7720	T9216	T9215	arg1Of	results,These
R7721	T9216	T9217	arg1Of	results,indicated
R7722	T9219	T9220	arg1Of	Tat,enhanced
R7723	T9219	T9230	arg1Of	Tat,affecting
R7724	T9220	T9217	arg2Of	enhanced,indicated
R7725	T9220	T9218	arg1Of	enhanced,that
R7726	T9220	T9224	arg1Of	enhanced,in
R7727	T9220	T9229	arg1Of	enhanced,without
R7728	T9223	T9220	arg2Of	activity,enhanced
R7729	T9223	T9221	arg1Of	activity,the
R7730	T9223	T9222	arg1Of	activity,NF-κB
R7731	T9225	T9226	arg1Of	un-stimulated,and
R7732	T9227	T9226	arg2Of	PMA-stimulated,and
R7733	T9228	T9224	arg2Of	cells,in
R7734	T9228	T9225	arg1Of	cells,un-stimulated
R7735	T9228	T9227	arg1Of	cells,PMA-stimulated
R7736	T9230	T9229	arg2Of	affecting,without
R7737	T9233	T9232	arg1Of	activity,IKK
R7738	T9233	T9234	arg1Of	activity,and
R7739	T9234	T9230	arg2Of	and,affecting
R7740	T9234	T9231	arg1Of	and,the
R7741	T9236	T9234	arg2Of	content,and
R7742	T9236	T9235	arg1Of	content,IκB-α
R7743	T9238	T9240	arg1Of	we,found
R7744	T9240	T9237	arg2Of	found,As
R7745	T9240	T9239	arg1Of	found,previously
R7746	T9242	T9243	arg1Of	Tat,binds
R7747	T9243	T9240	arg2Of	binds,found
R7748	T9243	T9241	arg1Of	binds,that
R7749	T9243	T9244	arg1Of	binds,to
R7750	T9247	T9244	arg2Of	ankyrin,to
R7751	T9247	T9245	arg1Of	ankyrin,the
R7752	T9247	T9246	arg1Of	ankyrin,sixth
R7753	T9247	T9248	arg1Of	ankyrin,of
R7754	T9249	T9248	arg2Of	IκB-α,of
R7755	T9249	T9250	arg1Of	IκB-α,(
R7756	T9251	T9250	arg2Of	"50,51",(
R7757	T9252	T9250	arg3Of	),(
R7758	T9254	T9255	arg1Of	we,tested
R7759	T9255	T9237	arg1Of	tested,As
R7760	T9255	T9253	arg1Of	tested,","
R7761	T9257	T9258	arg1Of	Tat,counteracted
R7762	T9258	T9255	arg2Of	counteracted,tested
R7763	T9258	T9256	arg1Of	counteracted,whether
R7764	T9260	T9258	arg2Of	generation,counteracted
R7765	T9260	T9259	arg1Of	generation,the
R7766	T9260	T9261	arg1Of	generation,of
R7767	T9264	T9261	arg2Of	complex,of
R7768	T9264	T9262	arg1Of	complex,the
R7769	T9264	T9263	arg1Of	complex,IκB-α/NF-κB
R7770	T9267	T9265	arg2Of	end,To
R7771	T9267	T9266	arg1Of	end,this
R7772	T9270	T9269	arg1Of	association,the
R7773	T9270	T9271	arg1Of	association,of
R7774	T9270	T9273	arg1Of	association,with
R7775	T9270	T9275	arg1Of	association,was
R7776	T9270	T9276	arg2Of	association,analysed
R7777	T9272	T9271	arg2Of	IκB-α,of
R7778	T9274	T9273	arg2Of	p65,with
R7779	T9276	T9265	arg1Of	analysed,To
R7780	T9276	T9268	arg1Of	analysed,","
R7781	T9276	T9275	arg2Of	analysed,was
R7782	T9276	T9278	arg1Of	analysed,vivo
R7783	T9276	T9279	arg1Of	analysed,at
R7784	T9276	T9283	arg1Of	analysed,after
R7785	T9278	T9277	arg1Of	vivo,in
R7786	T9280	T9281	arg1Of	0,and
R7787	T9281	T9279	arg2Of	and,at
R7788	T9282	T9281	arg2Of	240 min,and
R7789	T9285	T9283	arg2Of	PMA,after
R7790	T9285	T9284	arg1Of	PMA,short-pulse
R7791	T9285	T9286	arg1Of	PMA,","
R7792	T9285	T9287	arg1Of	PMA,which
R7793	T9285	T9288	arg1Of	PMA,corresponded
R7794	T9288	T9289	arg1Of	corresponded,to
R7795	T9288	T9302	arg1Of	corresponded,","
R7796	T9288	T9303	arg1Of	corresponded,respectively
R7797	T9291	T9289	arg2Of	time,to
R7798	T9291	T9290	arg1Of	time,the
R7799	T9291	T9292	arg1Of	time,of
R7800	T9294	T9293	arg1Of	condition,un-stimulated
R7801	T9294	T9295	arg1Of	condition,and
R7802	T9295	T9292	arg2Of	and,of
R7803	T9298	T9297	arg1Of	novo,de
R7804	T9299	T9295	arg2Of	synthesis,and
R7805	T9299	T9296	arg1Of	synthesis,post-activation
R7806	T9299	T9298	arg1Of	synthesis,novo
R7807	T9299	T9300	arg1Of	synthesis,of
R7808	T9301	T9300	arg2Of	IκB-α,of
R7809	T9304	T9305	arg1Of	Coimmunoprecipitation,of
R7810	T9304	T9307	arg1Of	Coimmunoprecipitation,with
R7811	T9304	T9309	arg1Of	Coimmunoprecipitation,was
R7812	T9304	T9310	arg2Of	Coimmunoprecipitation,detected
R7813	T9304	T9330	arg1Of	Coimmunoprecipitation,was
R7814	T9304	T9331	arg2Of	Coimmunoprecipitation,halved
R7815	T9306	T9305	arg2Of	IκB-α,of
R7816	T9308	T9307	arg2Of	p65,with
R7817	T9310	T9309	arg2Of	detected,was
R7818	T9310	T9311	arg1Of	detected,at
R7819	T9310	T9329	arg1Of	detected,and
R7820	T9312	T9313	arg1Of	0-,and
R7821	T9313	T9311	arg2Of	and,at
R7822	T9314	T9313	arg2Of	240-min,and
R7823	T9314	T9315	arg1Of	240-min,post-treatment
R7824	T9315	T9316	arg1Of	post-treatment,in
R7825	T9318	T9316	arg2Of	cells,in
R7826	T9318	T9317	arg1Of	cells,Tat-negative
R7827	T9318	T9319	arg1Of	cells,(
R7828	T9321	T9319	arg2Of	2D,(
R7829	T9321	T9320	arg1Of	2D,Figure
R7830	T9321	T9322	arg1Of	2D,","
R7831	T9324	T9325	arg1Of	1,and
R7832	T9325	T9322	arg2Of	and,","
R7833	T9325	T9323	arg1Of	and,lanes
R7834	T9326	T9325	arg2Of	3,and
R7835	T9327	T9319	arg3Of	),(
R7836	T9329	T9328	arg1Of	and,","
R7837	T9329	T9350	arg1Of	and,","
R7838	T9329	T9351	modOf	and,indicating
R7839	T9331	T9329	arg2Of	halved,and
R7840	T9331	T9330	arg2Of	halved,was
R7841	T9331	T9332	arg1Of	halved,in
R7842	T9334	T9332	arg2Of	cells,in
R7843	T9334	T9333	arg1Of	cells,Tat-positive
R7844	T9334	T9335	arg1Of	cells,","
R7845	T9334	T9336	arg1Of	cells,where
R7846	T9334	T9341	arg1Of	cells,(
R7847	T9337	T9338	arg1Of	Tat,coimmunoprecipitated
R7848	T9338	T9336	arg2Of	coimmunoprecipitated,where
R7849	T9338	T9339	arg1Of	coimmunoprecipitated,with
R7850	T9340	T9339	arg2Of	IκB-α,with
R7851	T9343	T9341	arg2Of	2D,(
R7852	T9343	T9342	arg1Of	2D,Figure
R7853	T9343	T9344	arg1Of	2D,","
R7854	T9346	T9347	arg1Of	2,and
R7855	T9347	T9344	arg2Of	and,","
R7856	T9347	T9345	arg1Of	and,lanes
R7857	T9348	T9347	arg2Of	4,and
R7858	T9349	T9341	arg3Of	),(
R7859	T9353	T9354	arg1Of	Tat,competed
R7860	T9354	T9351	arg2Of	competed,indicating
R7861	T9354	T9352	arg1Of	competed,that
R7862	T9357	T9354	arg2Of	binding,competed
R7863	T9357	T9355	arg1Of	binding,the
R7864	T9357	T9356	arg1Of	binding,IκB-α
R7865	T9357	T9358	arg1Of	binding,to
R7866	T9359	T9358	arg2Of	p65,to
R19354	T24359	T24360	arg1Of	Tat,enhances
R19355	T24359	T24368	arg1Of	Tat,associating
R19356	T24359	T24372	arg1Of	Tat,counteracting
R19357	T24360	T24367	arg1Of	enhances,by
R19358	T24363	T24360	arg2Of	activity,enhances
R19359	T24363	T24361	arg1Of	activity,the
R19360	T24363	T24362	arg1Of	activity,NF-κB
R19361	T24363	T24364	arg1Of	activity,following
R19362	T24366	T24364	arg2Of	stimulation,following
R19363	T24366	T24365	arg1Of	stimulation,PMA
R19364	T24368	T24369	arg1Of	associating,with
R19365	T24368	T24371	arg1Of	associating,and
R19366	T24370	T24369	arg2Of	IκB-α,with
R19367	T24371	T24367	arg2Of	and,by
R19368	T24372	T24371	arg2Of	counteracting,and
R19369	T24375	T24372	arg2Of	repression,counteracting
R19370	T24375	T24373	arg1Of	repression,the
R19371	T24375	T24374	arg1Of	repression,NF-κB
R19372	T24377	T24376	arg1Of	cells,HeLa
R19373	T24377	T24378	arg1Of	cells,(
R19374	T24377	T24381	arg1Of	cells,were
R19375	T24377	T24382	arg2Of	cells,transfected
R19376	T24379	T24378	arg2Of	5 × 106,(
R19377	T24380	T24378	arg3Of	),(
R19378	T24382	T24381	arg2Of	transfected,were
R19379	T24382	T24383	arg1Of	transfected,with
R19380	T24382	T24393	arg1Of	transfected,and
R19381	T24384	T24385	arg1Of	p3xFLAG-Tat,or
R19382	T24385	T24383	arg2Of	or,with
R19383	T24388	T24385	arg2Of	vector,or
R19384	T24388	T24386	arg1Of	vector,p3xFLAG
R19385	T24388	T24387	arg1Of	vector,empty
R19386	T24388	T24389	arg1Of	vector,(
R19387	T24390	T24389	arg2Of	5 µg,(
R19388	T24391	T24389	arg3Of	),(
R19389	T24393	T24392	arg1Of	and,","
R19390	T24394	T24396	arg1Of	48 h,were
R19391	T24394	T24397	arg2Of	48 h,stimulated
R19392	T24394	T24406	arg1Of	48 h,left
R19393	T24394	T24409	arg1Of	48 h,washed
R19394	T24394	T24414	arg1Of	48 h,harvested
R19395	T24397	T24396	arg2Of	stimulated,were
R19396	T24397	T24398	arg1Of	stimulated,with
R19397	T24397	T24405	arg1Of	stimulated,or
R19398	T24399	T24398	arg2Of	PMA,with
R19399	T24399	T24400	arg1Of	PMA,(
R19400	T24401	T24400	arg2Of	5 min,(
R19401	T24401	T24402	arg1Of	5 min,","
R19402	T24403	T24402	arg2Of	20 ng/ml,","
R19403	T24404	T24400	arg3Of	),(
R19404	T24405	T24408	arg1Of	or,","
R19405	T24406	T24405	arg2Of	left,or
R19406	T24407	T24406	arg2Of	unstimulated,left
R19407	T24408	T24393	arg2Of	",",and
R19408	T24408	T24395	arg1Of	",",later
R19409	T24409	T24410	arg1Of	washed,twice
R19410	T24409	T24411	arg1Of	washed,with
R19411	T24409	T24413	arg1Of	washed,and
R19412	T24412	T24411	arg2Of	DMEM,with
R19413	T24413	T24408	arg2Of	and,","
R19414	T24414	T24413	arg2Of	harvested,and
R19415	T24414	T24415	arg1Of	harvested,at
R19416	T24418	T24415	arg2Of	times,at
R19417	T24418	T24416	arg1Of	times,the
R19418	T24418	T24417	arg1Of	times,indicated
R19419	T24419	T24420	arg1Of	Nuclear,and
R19420	T24421	T24420	arg2Of	cytosolic,and
R19421	T24422	T24419	arg1Of	extracts,Nuclear
R19422	T24422	T24421	arg1Of	extracts,cytosolic
R19423	T24422	T24423	arg1Of	extracts,were
R19424	T24422	T24424	arg2Of	extracts,prepared
R19425	T24424	T24423	arg2Of	prepared,were
R19426	T24424	T24425	arg1Of	prepared,for
R19427	T24427	T24425	arg2Of	analysis.,for
R19428	T24427	T24426	arg1Of	analysis.,further
R19429	T24427	T24433	modOf	analysis.,were
R19430	T24429	T24428	arg2Of	A,(
R19431	T24430	T24428	arg3Of	),(
R19432	T24432	T24428	arg1Of	extracts,(
R19433	T24432	T24431	arg1Of	extracts,Nuclear
R19434	T24432	T24433	arg1Of	extracts,were
R19435	T24432	T24434	arg2Of	extracts,analysed
R19436	T24434	T24433	arg2Of	analysed,were
R19437	T24434	T24435	arg1Of	analysed,for
R19438	T24437	T24438	arg1Of	p65,","
R19439	T24438	T24440	arg1Of	",",and
R19440	T24439	T24438	arg2Of	p50,","
R19441	T24441	T24440	arg2Of	FLAG-Tat,and
R19442	T24442	T24435	arg2Of	binding,for
R19443	T24442	T24436	arg1Of	binding,the
R19444	T24442	T24437	arg1Of	binding,p65
R19445	T24442	T24439	arg1Of	binding,p50
R19446	T24442	T24441	arg1Of	binding,FLAG-Tat
R19447	T24442	T24443	arg1Of	binding,to
R19448	T24447	T24443	arg2Of	oligonucleotide,to
R19449	T24447	T24444	arg1Of	oligonucleotide,the
R19450	T24447	T24445	arg1Of	oligonucleotide,NF-κB
R19451	T24447	T24446	arg1Of	oligonucleotide,double-stranded
R19452	T24447	T24448	arg1Of	oligonucleotide,using
R19453	T24455	T24449	arg1Of	kit,the
R19454	T24455	T24450	arg1Of	kit,NF-κB
R19455	T24455	T24451	arg1Of	kit,Transcription
R19456	T24455	T24452	arg1Of	kit,Factor
R19457	T24455	T24453	arg1Of	kit,ELISA
R19458	T24455	T24454	arg1Of	kit,assay
R19459	T24455	T24456	arg1Of	kit,(
R19460	T24455	T24469	modOf	kit,20 µ
R19461	T24455	T24480	arg1Of	kit,ester
R19462	T24455	T24488	arg1Of	kit,F
R19463	T24457	T24456	arg2Of	Cayman,(
R19464	T24458	T24456	arg3Of	),(
R19465	T24460	T24459	arg2Of	(,.
R19466	T24461	T24459	arg3Of	B,.
R19467	T24462	T24463	arg1Of	) Nucle,ar
R19468	T24464	T24463	arg2Of	and cytos,ar
R19469	T24465	T24459	arg1Of	olic ext,.
R19470	T24465	T24462	arg1Of	olic ext,) Nucle
R19471	T24465	T24464	arg1Of	olic ext,and cytos
R19472	T24465	T24466	arg1Of	olic ext,r
R19473	T24465	T24469	arg1Of	olic ext,20 µ
R19474	T24465	T24470	arg2Of	olic ext,g) were s
R19475	T24465	T24476	arg2Of	olic ext,SDS–PAGE
R19476	T24467	T24466	arg2Of	acts ,r
R19477	T24468	T24466	arg3Of	(,r
R19478	T24470	T24475	arg1Of	g) were s,2%
R19479	T24472	T24473	arg1Of	ar,a
R19480	T24474	T24470	arg1Of	ted by 1,g) were s
R19481	T24474	T24471	arg2Of	ted by 1,ep
R19482	T24474	T24472	arg1Of	ted by 1,ar
R19483	T24475	T24469	arg2Of	2% ,20 µ
R19484	T24476	T24475	arg2Of	SDS–PAGE,2%
R19485	T24479	T24476	arg1Of	sed by w,SDS–PAGE
R19486	T24479	T24477	arg2Of	sed by w,an
R19487	T24479	T24478	arg1Of	sed by w,d analy
R19488	T24482	T24483	arg1Of	lotting ,u
R19489	T24483	T24485	arg1Of	u,a
R19490	T24484	T24483	arg2Of	sing the ,u
R19491	T24486	T24485	arg2Of	"nti-p65, ant",a
R19492	T24487	T24480	arg2Of	i-,ester
R19493	T24487	T24481	arg1Of	i-,n b
R19494	T24487	T24482	arg1Of	i-,lotting
R19495	T24487	T24484	arg1Of	i-,sing the
R19496	T24487	T24486	arg1Of	i-,"nti-p65, ant"
R19497	T24488	T24448	arg2Of	F,using
R19498	T24489	T24490	arg1Of	"LAG, anti-",His
R19499	T24491	T24490	arg2Of	"tone H1, anti-IκB-",His
R19500	T24492	T24488	arg2Of	α and anti,F
R19501	T24492	T24489	arg1Of	α and anti,"LAG, anti-"
R19502	T24492	T24491	arg1Of	α and anti,"tone H1, anti-IκB-"
R19503	T24494	T24493	arg1Of	values,Densitometry
R19504	T24494	T24495	arg1Of	values,(
R19505	T24494	T24498	arg1Of	values,of
R19506	T24494	T24501	arg1Of	values,were
R19507	T24494	T24502	arg2Of	values,expressed
R19508	T24496	T24495	arg2Of	D,(
R19509	T24497	T24495	arg3Of	),(
R19510	T24500	T24498	arg2Of	bands,of
R19511	T24500	T24499	arg1Of	bands,the
R19512	T24502	T24501	arg2Of	expressed,were
R19513	T24502	T24503	arg1Of	expressed,as
R19514	T24502	T24554	arg1Of	expressed,t
R19515	T24505	T24503	arg2Of	increase,as
R19516	T24505	T24504	arg1Of	increase,fold
R19517	T24505	T24506	arg1Of	increase,above
R19518	T24505	T24518	modOf	increase,ity
R19519	T24508	T24506	arg2Of	control,above
R19520	T24508	T24507	arg1Of	control,the
R19521	T24510	T24509	arg2Of	lane,(
R19522	T24510	T24511	arg1Of	lane,1
R19523	T24512	T24509	arg3Of	),(
R19524	T24514	T24513	arg2Of	(,.
R19525	T24515	T24513	arg3Of	C,.
R19526	T24517	T24509	arg1Of	KK activ,(
R19527	T24517	T24513	arg1Of	KK activ,.
R19528	T24517	T24516	arg1Of	KK activ,) I
R19529	T24517	T24518	arg1Of	KK activ,ity
R19530	T24517	T24519	arg2Of	KK activ,was meas
R19531	T24519	T24518	arg2Of	was meas,ity
R19532	T24519	T24520	arg1Of	was meas,ur
R19533	T24519	T24526	arg1Of	was meas,10
R19534	T24522	T24520	arg2Of	osolic e,ur
R19535	T24522	T24521	arg1Of	osolic e,ed in cyt
R19536	T24522	T24523	arg1Of	osolic e,x
R19537	T24524	T24523	arg2Of	tracts,x
R19538	T24525	T24523	arg3Of	(,x
R19539	T24527	T24526	arg2Of	0 µg),10
R19540	T24531	T24527	arg2Of	IKK k,0 µg)
R19541	T24531	T24528	arg1Of	IKK k,by usi
R19542	T24531	T24529	arg1Of	IKK k,ng
R19543	T24531	T24530	arg1Of	IKK k,HTScan
R19544	T24531	T24532	arg1Of	IKK k,i
R19545	T24531	T24545	modOf	IKK k,cell
R19546	T24535	T24532	arg2Of	ll Signali,i
R19547	T24535	T24533	arg1Of	ll Signali,nase
R19548	T24535	T24534	arg1Of	ll Signali,assay (Ce
R19549	T24536	T24532	arg3Of	n,i
R19550	T24538	T24537	arg2Of	T,g
R19551	T24539	T24537	arg3Of	e,g
R19552	T24541	T24537	arg1Of	logy),g
R19553	T24541	T24540	arg1Of	logy),chno
R19554	T24541	T24542	arg1Of	logy),.
R19555	T24541	T24545	arg1Of	logy),cell
R19556	T24541	T24546	arg2Of	logy),s (5 × 106)
R19557	T24543	T24542	arg2Of	(D) HeL,.
R19558	T24544	T24542	arg3Of	a,.
R19559	T24546	T24545	arg2Of	s (5 × 106),cell
R19560	T24546	T24547	arg1Of	s (5 × 106),were
R19561	T24546	T24549	arg1Of	s (5 × 106),ansfect
R19562	T24547	T24548	arg1Of	were,tr
R19563	T24549	T24548	arg2Of	ansfect,tr
R19564	T24550	T24547	arg2Of	ed with or ,were
R19565	T24550	T24549	arg2Of	ed with or ,ansfect
R19566	T24550	T24551	arg1Of	ed with or ,w
R19567	T24552	T24551	arg2Of	itho,w
R19568	T24553	T24551	arg3Of	u,w
R19569	T24556	T24555	arg1Of	LAG-T,p3xF
R19570	T24558	T24559	arg1Of	t (5 ,µg);
R19571	T24558	T24560	arg2Of	t (5 ,48 h later
R19572	T24558	T24569	arg1Of	t (5 ,h PM
R19573	T24560	T24559	arg2Of	48 h later,µg);
R19574	T24560	T24561	arg1Of	48 h later,", ce"
R19575	T24560	T24568	arg1Of	48 h later,it
R19576	T24562	T24561	arg2Of	lls,", ce"
R19577	T24562	T24563	arg1Of	lls,w
R19578	T24564	T24563	arg2Of	ere s,w
R19579	T24564	T24565	arg1Of	ere s,t
R19580	T24566	T24565	arg2Of	imulated,t
R19581	T24567	T24563	arg3Of	w,w
R19582	T24568	T24554	arg2Of	it,t
R19583	T24568	T24556	arg1Of	it,LAG-T
R19584	T24568	T24557	arg1Of	it,a
R19585	T24569	T24568	arg2Of	h PM,it
R19586	T24569	T24571	arg1Of	h PM,
R19587	T24570	T24569	arg2Of	"A (5 min, 20",h PM
R19588	T24570	T24572	arg2Of	"A (5 min, 20",ng/ml)
R19589	T24570	T24578	arg2Of	"A (5 min, 20","ted, wash"
R19590	T24572	T24573	arg1Of	ng/ml),or le
R19591	T24572	T24574	arg1Of	ng/ml),ft u
R19592	T24572	T24577	arg1Of	ng/ml),ula
R19593	T24575	T24574	arg2Of	nsti,ft u
R19594	T24577	T24569	arg3Of	ula,h PM
R19595	T24577	T24576	arg1Of	ula,m
R19596	T24578	T24577	arg2Of	"ted, wash",ula
R19597	T24578	T24579	arg1Of	"ted, wash",ed tw
R19598	T24580	T24579	arg2Of	ice wit,ed tw
R19599	T24583	T24581	arg1Of	extracts,Whole
R19600	T24583	T24582	arg1Of	extracts,cell
R19601	T24583	T24584	arg1Of	extracts,(
R19602	T24583	T24587	arg1Of	extracts,were
R19603	T24583	T24588	arg2Of	extracts,immunoprecipitated
R19604	T24585	T24584	arg2Of	1 mg,(
R19605	T24586	T24584	arg3Of	),(
R19606	T24588	T24587	arg2Of	immunoprecipitated,were
R19607	T24588	T24589	arg1Of	immunoprecipitated,with
R19608	T24593	T24589	arg2Of	antibody,with
R19609	T24593	T24590	arg1Of	antibody,protein
R19610	T24593	T24591	arg1Of	antibody,G-Sepharose-coupled
R19611	T24593	T24592	arg1Of	antibody,anti-IκB-α
R19612	T24594	T24595	arg1Of	Immunocomplexes,were
R19613	T24594	T24596	arg2Of	Immunocomplexes,separated
R19614	T24594	T24602	arg2Of	Immunocomplexes,analysed
R19615	T24596	T24601	arg1Of	separated,and
R19616	T24598	T24599	arg1Of	12,%
R19617	T24600	T24596	arg1Of	SDS–PAGE,separated
R19618	T24600	T24597	arg2Of	SDS–PAGE,by
R19619	T24600	T24598	arg1Of	SDS–PAGE,12
R19620	T24601	T24595	arg2Of	and,were
R19621	T24602	T24601	arg2Of	analysed,and
R19622	T24605	T24602	arg1Of	blotting,analysed
R19623	T24605	T24603	arg2Of	blotting,by
R19624	T24605	T24604	arg1Of	blotting,western
R19625	T24605	T24606	arg1Of	blotting,with
R19626	T24607	T24608	arg1Of	anti-p65,","
R19627	T24608	T24610	arg1Of	",",and
R19628	T24609	T24608	arg2Of	anti-FLAG,","
R19629	T24611	T24610	arg2Of	anti-IκB-α,and
R19630	T24612	T24606	arg2Of	antibodies,with
R19631	T24612	T24607	arg1Of	antibodies,anti-p65
R19632	T24612	T24609	arg1Of	antibodies,anti-FLAG
R19633	T24612	T24611	arg1Of	antibodies,anti-IκB-α
R19634	T24614	T24613	arg1Of	values,Densitometry
R19635	T24614	T24615	arg1Of	values,(
R19636	T24614	T24618	arg1Of	values,of
R19637	T24614	T24621	arg1Of	values,were
R19638	T24614	T24622	arg2Of	values,expressed
R19639	T24616	T24615	arg2Of	D,(
R19640	T24617	T24615	arg3Of	),(
R19641	T24620	T24618	arg2Of	bands,of
R19642	T24620	T24619	arg1Of	bands,the
R19643	T24622	T24621	arg2Of	expressed,were
R19644	T24622	T24623	arg1Of	expressed,as
R19645	T24625	T24623	arg2Of	increase,as
R19646	T24625	T24624	arg1Of	increase,fold
R19647	T24625	T24626	arg1Of	increase,above
R19648	T24628	T24626	arg2Of	control,above
R19649	T24628	T24627	arg1Of	control,the
R19650	T24628	T24629	arg1Of	control,(
R19651	T24630	T24629	arg2Of	lane,(
R19652	T24630	T24631	arg1Of	lane,1
R19653	T24632	T24629	arg3Of	),(
R19654	T24633	T24634	arg1Of	Values,(
R19655	T24633	T24639	arg1Of	Values,are
R19656	T24633	T24640	arg2Of	Values,shown
R19657	T24635	T24634	arg2Of	mean ± SE,(
R19658	T24635	T24636	arg1Of	mean ± SE,","
R19659	T24637	T24636	arg2Of	n = 3,","
R19660	T24638	T24634	arg3Of	),(
R19661	T24640	T24639	arg2Of	shown,are

bionlp-st-ge-2016-coref

Id	Subject	Object	Predicate	Lexical cue
T9360	699-701	Anaphor	denotes	it
T9361	547-557	Antecedent	denotes	expression
T9362	2686-2690	Anaphor	denotes	they
T9363	2588-2595	Antecedent	denotes	binding
T9364	2608-2611	Antecedent	denotes	p65
T9365	4447-4451	Anaphor	denotes	they
T9366	4375-4378	Antecedent	denotes	p65
T9367	4580-4583	Anaphor	denotes	its
T9368	4514-4517	Antecedent	denotes	Tat
R7867	T9360	T9361	boundBy	it,expression
R7868	T9362	T9363	boundBy	they,binding
R7869	T9362	T9364	boundBy	they,p65
R7870	T9365	T9366	boundBy	they,p65
R7871	T9367	T9368	boundBy	its,Tat

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T9532	0-3	NNP	denotes	Tat
T9533	4-12	VBZ	denotes	enhances
T9534	13-16	DT	denotes	the
T9535	17-22	JJ	denotes	NF-κB
T9536	23-31	NN	denotes	activity
T9537	32-34	IN	denotes	by
T9538	35-44	VBG	denotes	hijacking
T9539	45-50	NNP	denotes	IκB-α
T9540	51-54	CC	denotes	and
T9541	55-65	VBG	denotes	inhibiting
T9542	66-69	DT	denotes	the
T9543	70-85	NN	denotes	post-activation
T9544	86-90	VB	denotes	turn
T9545	91-94	RB	denotes	off
T9546	95-97	IN	denotes	of
T9547	98-103	NNP	denotes	NF-κB
T9548	104-106	PRP	denotes	We
T9549	107-115	VBD	denotes	analysed
T9550	116-119	DT	denotes	the
T9551	120-127	NN	denotes	kinetic
T9552	128-130	IN	denotes	of
T9553	131-136	JJ	denotes	NF-κB
T9554	137-147	NN	denotes	activation
T9555	148-150	IN	denotes	in
T9556	151-157	JJ	denotes	single
T9557	158-163	NN	denotes	round
T9558	164-169	NN	denotes	HIV-1
T9559	170-179	NN	denotes	infection
T9560	180-182	IN	denotes	by
T9561	183-193	VBG	denotes	modulating
T9562	194-197	DT	denotes	the
T9563	198-208	NN	denotes	expression
T9564	209-211	IN	denotes	of
T9565	212-215	NNP	denotes	Tat
T9566	216-220	IN	denotes	with
T9567	221-224	NNP	denotes	RNA
T9568	225-237	NN	denotes	interference
T9569	237-238	.	denotes	.
T9570	239-245	NNP	denotes	Jurkat
T9571	246-251	NNS	denotes	cells
T9572	252-256	VBD	denotes	were
T9573	257-268	VBN	denotes	transfected
T9574	269-273	IN	denotes	with
T9575	274-279	NNP	denotes	siRNA
T9576	280-283	NNP	denotes	Tat
T9577	283-284	,	denotes	,
T9578	285-290	NNP	denotes	siRNA
T9579	291-298	NN	denotes	control
T9580	298-299	,	denotes	,
T9581	300-302	CC	denotes	or
T9582	303-307	VBD	denotes	left
T9583	308-321	JJ	denotes	untransfected
T9584	321-322	,	denotes	,
T9585	323-326	CC	denotes	and
T9586	327-329	CD	denotes	24
T9587	330-331	NN	denotes	h
T9588	332-337	RB	denotes	later
T9589	338-343	NNS	denotes	cells
T9590	344-348	VBD	denotes	were
T9591	349-357	VBN	denotes	infected
T9592	358-362	IN	denotes	with
T9593	363-367	CD	denotes	HXB2
T9594	368-383	JJ	denotes	Env-pseudotyped
T9595	384-389	NN	denotes	NL4-3
T9596	389-390	.	denotes	.
T9597	390-395	NNP	denotes	Luc.R
T9598	395-396	NNP	denotes	−
T9599	396-397	NNP	denotes	E
T9600	397-398	VBD	denotes	−
T9601	399-406	NNS	denotes	virions
T9602	406-407	.	denotes	.
T9603	408-410	IN	denotes	By
T9604	411-427	JJ	denotes	cytofluorimetric
T9605	428-436	NN	denotes	analysis
T9606	436-437	,	denotes	,
T9607	438-439	RB	denotes	∼
T9608	439-441	CD	denotes	40
T9609	441-442	NN	denotes	%
T9610	443-445	IN	denotes	of
T9611	446-451	NNS	denotes	cells
T9612	452-455	VBD	denotes	was
T9613	456-473	JJ	denotes	siRNA-transfected
T9614	474-477	CC	denotes	and
T9615	478-492	JJ	denotes	HIV-1-infected
T9616	493-495	IN	denotes	at
T9617	496-500	JJ	denotes	12-h
T9618	501-515	NN	denotes	post-infection
T9619	516-517	-LRB-	denotes	(
T9620	517-530	NNP	denotes	Supplementary
T9621	531-537	NNP	denotes	Figure
T9622	538-540	NNP	denotes	S1
T9623	540-541	-RRB-	denotes	)
T9624	541-542	.	denotes	.
T9625	543-546	DT	denotes	The
T9626	547-557	NN	denotes	expression
T9627	558-560	IN	denotes	of
T9628	561-564	NNP	denotes	Tat
T9629	565-568	VBD	denotes	was
T9630	569-577	VBN	denotes	detected
T9631	578-580	IN	denotes	at
T9632	581-584	JJ	denotes	1-h
T9633	585-599	NN	denotes	post-infection
T9634	600-603	CC	denotes	and
T9635	604-617	RB	denotes	progressively
T9636	618-627	VBD	denotes	increased
T9637	628-630	RB	denotes	up
T9638	631-633	TO	denotes	to
T9639	634-636	CD	denotes	12
T9640	637-638	NN	denotes	h
T9641	639-641	IN	denotes	in
T9642	642-655	JJ	denotes	untransfected
T9643	656-659	CC	denotes	and
T9644	660-665	JJ	denotes	siRNA
T9645	666-685	JJ	denotes	control-transfected
T9646	686-691	NNS	denotes	cells
T9647	691-692	,	denotes	,
T9648	693-698	IN	denotes	while
T9649	699-701	PRP	denotes	it
T9650	702-705	VBD	denotes	was
T9651	706-712	RB	denotes	barely
T9652	713-721	VBN	denotes	detected
T9653	722-724	IN	denotes	in
T9654	725-730	NNP	denotes	siRNA
T9655	731-746	VBD	denotes	Tat-transfected
T9656	747-752	NNS	denotes	cells
T9657	753-754	-LRB-	denotes	(
T9658	754-760	NN	denotes	Figure
T9659	761-763	CD	denotes	1A
T9660	763-764	-RRB-	denotes	)
T9661	764-765	.	denotes	.
T9662	766-769	DT	denotes	The
T9663	770-783	JJ	denotes	LTR-dependent
T9664	784-794	NN	denotes	luciferase
T9665	795-805	NN	denotes	expression
T9666	806-809	VBD	denotes	was
T9667	810-814	RB	denotes	also
T9668	815-828	RB	denotes	progressively
T9669	829-836	VBD	denotes	induced
T9670	837-839	IN	denotes	at
T9671	840-841	LS	denotes	1
T9672	841-842	:	denotes	-
T9673	843-845	TO	denotes	to
T9674	846-850	JJ	denotes	12-h
T9675	851-865	NN	denotes	post-infection
T9676	866-868	IN	denotes	in
T9677	869-882	JJ	denotes	untransfected
T9678	883-886	CC	denotes	and
T9679	887-892	JJ	denotes	siRNA
T9680	893-912	JJ	denotes	control-transfected
T9681	913-918	NNS	denotes	cells
T9682	918-919	,	denotes	,
T9683	920-925	IN	denotes	while
T9684	926-928	PRP	denotes	it
T9685	929-932	VBD	denotes	was
T9686	933-939	RB	denotes	barely
T9687	940-948	VBN	denotes	observed
T9688	949-951	IN	denotes	in
T9689	952-957	NNP	denotes	siRNA
T9690	958-973	VBD	denotes	Tat-transfected
T9691	974-979	NNS	denotes	cells
T9692	979-980	,	denotes	,
T9693	981-983	IN	denotes	as
T9694	984-992	VBN	denotes	expected
T9695	993-996	IN	denotes	for
T9696	997-1000	DT	denotes	the
T9697	1001-1014	JJ	denotes	Tat-dependent
T9698	1015-1030	NN	denotes	transactivation
T9699	1031-1033	IN	denotes	of
T9700	1034-1037	DT	denotes	the
T9701	1038-1043	NNP	denotes	HIV-1
T9702	1044-1047	NNP	denotes	LTR
T9703	1048-1049	-LRB-	denotes	(
T9704	1049-1055	NNP	denotes	Figure
T9705	1056-1058	NNP	denotes	1A
T9706	1058-1059	-RRB-	denotes	)
T9707	1059-1060	.	denotes	.
T9708	2254-2257	DT	denotes	The
T9709	2258-2269	JJ	denotes	progressive
T9710	2270-2278	NN	denotes	increase
T9711	2279-2281	IN	denotes	in
T9712	2282-2287	JJ	denotes	NF-κB
T9713	2288-2296	NN	denotes	activity
T9714	2296-2297	,	denotes	,
T9715	2298-2300	IN	denotes	as
T9716	2301-2309	VBN	denotes	measured
T9717	2310-2312	IN	denotes	by
T9718	2313-2316	NNS	denotes	p65
T9719	2317-2320	JJ	denotes	p50
T9720	2321-2328	JJ	denotes	binding
T9721	2329-2331	TO	denotes	to
T9722	2332-2334	DT	denotes	an
T9723	2335-2340	JJ	denotes	NF-κB
T9724	2341-2350	NN	denotes	consensus
T9725	2351-2366	NN	denotes	oligonucleotide
T9726	2367-2368	-LRB-	denotes	(
T9727	2368-2374	NN	denotes	Figure
T9728	2375-2377	NN	denotes	1B
T9729	2377-2378	-RRB-	denotes	)
T9730	2379-2382	CC	denotes	and
T9731	2383-2390	JJ	denotes	nuclear
T9732	2391-2394	NNS	denotes	p65
T9733	2395-2396	-LRB-	denotes	(
T9734	2396-2402	NN	denotes	Figure
T9735	2403-2405	CD	denotes	1C
T9736	2405-2406	,	denotes	,
T9737	2407-2414	NN	denotes	nucleus
T9738	2414-2415	-RRB-	denotes	)
T9739	2415-2416	,	denotes	,
T9740	2417-2420	VBD	denotes	was
T9741	2421-2429	VBN	denotes	observed
T9742	2430-2432	IN	denotes	at
T9743	2433-2434	LS	denotes	1
T9744	2434-2435	:	denotes	-
T9745	2436-2438	TO	denotes	to
T9746	2439-2442	JJ	denotes	3-h
T9747	2443-2457	NN	denotes	post-infection
T9748	2458-2460	IN	denotes	in
T9749	2461-2465	DT	denotes	both
T9750	2466-2478	JJ	denotes	Tat-positive
T9751	2479-2480	-LRB-	denotes	(
T9752	2480-2482	DT	denotes	no
T9753	2483-2488	NN	denotes	siRNA
T9754	2489-2491	CC	denotes	or
T9755	2492-2497	NN	denotes	siRNA
T9756	2498-2505	NN	denotes	control
T9757	2505-2506	-RRB-	denotes	)
T9758	2507-2510	CC	denotes	and
T9759	2511-2523	JJ	denotes	Tat-negative
T9760	2524-2525	-LRB-	denotes	(
T9761	2525-2530	NNP	denotes	siRNA
T9762	2531-2534	NNP	denotes	Tat
T9763	2534-2535	-RRB-	denotes	)
T9764	2536-2541	NNS	denotes	cells
T9765	2541-2542	:	denotes	;
T9766	2543-2550	RB	denotes	however
T9767	2550-2551	,	denotes	,
T9768	2552-2554	IN	denotes	at
T9769	2555-2559	JJ	denotes	12-h
T9770	2560-2574	NN	denotes	post-infection
T9771	2574-2575	,	denotes	,
T9772	2576-2579	DT	denotes	the
T9773	2580-2583	CD	denotes	p65
T9774	2584-2587	NNP	denotes	DNA
T9775	2588-2595	JJ	denotes	binding
T9776	2596-2599	CC	denotes	and
T9777	2600-2607	JJ	denotes	nuclear
T9778	2608-2611	NNS	denotes	p65
T9779	2612-2621	VBD	denotes	persisted
T9780	2622-2630	VBN	denotes	elevated
T9781	2631-2633	IN	denotes	in
T9782	2634-2642	NN	denotes	presence
T9783	2643-2645	IN	denotes	of
T9784	2646-2649	NNP	denotes	Tat
T9785	2650-2651	-LRB-	denotes	(
T9786	2651-2653	DT	denotes	no
T9787	2654-2659	NN	denotes	siRNA
T9788	2660-2663	CC	denotes	and
T9789	2664-2669	NN	denotes	siRNA
T9790	2670-2677	NN	denotes	control
T9791	2677-2678	-RRB-	denotes	)
T9792	2678-2679	,	denotes	,
T9793	2680-2685	IN	denotes	while
T9794	2686-2690	PRP	denotes	they
T9795	2691-2698	VBD	denotes	dropped
T9796	2699-2701	IN	denotes	in
T9797	2702-2709	NN	denotes	absence
T9798	2710-2712	IN	denotes	of
T9799	2713-2716	NNP	denotes	Tat
T9800	2717-2718	-LRB-	denotes	(
T9801	2718-2723	NNP	denotes	siRNA
T9802	2724-2727	NNP	denotes	Tat
T9803	2727-2728	-RRB-	denotes	)
T9804	2729-2730	-LRB-	denotes	(
T9805	2730-2736	NN	denotes	Figure
T9806	2737-2739	CD	denotes	1B
T9807	2740-2743	CC	denotes	and
T9808	2744-2745	NNP	denotes	C
T9809	2745-2746	-RRB-	denotes	)
T9810	2746-2747	.	denotes	.
T9811	2748-2753	NN	denotes	IκB-α
T9812	2754-2757	VBD	denotes	was
T9813	2758-2766	JJ	denotes	degraded
T9814	2767-2769	IN	denotes	in
T9815	2770-2773	DT	denotes	the
T9816	2774-2781	NN	denotes	cytosol
T9817	2782-2788	IN	denotes	within
T9818	2789-2792	JJ	denotes	3-h
T9819	2793-2807	NN	denotes	post-infection
T9820	2808-2811	CC	denotes	and
T9821	2812-2814	IN	denotes	de
T9822	2815-2819	JJ	denotes	novo
T9823	2820-2831	VBN	denotes	synthesized
T9824	2832-2834	IN	denotes	at
T9825	2835-2836	CD	denotes	6
T9826	2837-2838	NN	denotes	h
T9827	2839-2843	IN	denotes	with
T9828	2844-2848	JJ	denotes	full
T9829	2849-2862	NN	denotes	replenishment
T9830	2863-2865	IN	denotes	at
T9831	2866-2870	JJ	denotes	12-h
T9832	2871-2885	NN	denotes	post-infection
T9833	2886-2888	IN	denotes	in
T9834	2889-2893	DT	denotes	both
T9835	2894-2906	JJ	denotes	Tat-positive
T9836	2907-2910	CC	denotes	and
T9837	2911-2923	JJ	denotes	Tat-negative
T9838	2924-2929	NNS	denotes	cells
T9839	2930-2931	-LRB-	denotes	(
T9840	2931-2937	NN	denotes	Figure
T9841	2938-2940	CD	denotes	1C
T9842	2940-2941	,	denotes	,
T9843	2942-2949	NN	denotes	cytosol
T9844	2949-2950	-RRB-	denotes	)
T9845	2950-2951	.	denotes	.
T9846	2952-2964	RB	denotes	Consistently
T9847	2964-2965	,	denotes	,
T9848	2966-2969	DT	denotes	the
T9849	2970-2973	NNP	denotes	IKK
T9850	2974-2982	NN	denotes	activity
T9851	2983-2986	VBD	denotes	was
T9852	2987-2994	VBN	denotes	induced
T9853	2995-2997	IN	denotes	at
T9854	2998-3001	JJ	denotes	1-h
T9855	3002-3016	NN	denotes	post-infection
T9856	3016-3017	,	denotes	,
T9857	3018-3021	CC	denotes	and
T9858	3022-3031	VBD	denotes	decreased
T9859	3032-3034	IN	denotes	at
T9860	3035-3038	JJ	denotes	3-h
T9861	3039-3053	JJ	denotes	post-infection
T9862	3054-3067	RB	denotes	independently
T9863	3068-3070	IN	denotes	of
T9864	3071-3074	DT	denotes	the
T9865	3075-3083	NN	denotes	presence
T9866	3084-3086	IN	denotes	of
T9867	3087-3090	NNP	denotes	Tat
T9868	3091-3092	-LRB-	denotes	(
T9869	3092-3098	NNP	denotes	Figure
T9870	3099-3101	NNP	denotes	1D
T9871	3101-3102	-RRB-	denotes	)
T9872	3102-3103	.	denotes	.
T9873	3104-3111	JJ	denotes	Similar
T9874	3112-3120	NNS	denotes	kinetics
T9875	3121-3123	IN	denotes	of
T9876	3124-3129	JJ	denotes	NF-κB
T9877	3130-3138	NN	denotes	activity
T9878	3138-3139	,	denotes	,
T9879	3140-3143	NNP	denotes	IKK
T9880	3144-3154	NN	denotes	activation
T9881	3155-3158	CC	denotes	and
T9882	3159-3164	JJ	denotes	IκB-α
T9883	3165-3189	NN	denotes	degradation/re-synthesis
T9884	3190-3194	VBD	denotes	were
T9885	3195-3203	VBN	denotes	observed
T9886	3204-3206	IN	denotes	in
T9887	3207-3219	JJ	denotes	single-round
T9888	3220-3225	JJ	denotes	HIV-1
T9889	3226-3235	NN	denotes	infection
T9890	3236-3238	IN	denotes	of
T9891	3239-3244	NNP	denotes	PBMCs
T9892	3245-3250	VBG	denotes	using
T9893	3251-3252	DT	denotes	a
T9894	3253-3261	NN	denotes	cocktail
T9895	3262-3264	IN	denotes	of
T9896	3265-3279	NNP	denotes	Azidothymidine
T9897	3280-3281	-LRB-	denotes	(
T9898	3281-3284	NNP	denotes	AZT
T9899	3284-3285	-RRB-	denotes	)
T9900	3286-3289	CC	denotes	and
T9901	3290-3300	NNP	denotes	Lamivudine
T9902	3301-3302	-LRB-	denotes	(
T9903	3302-3305	NNP	denotes	3TC
T9904	3305-3306	-RRB-	denotes	)
T9905	3307-3309	IN	denotes	as
T9906	3310-3317	NN	denotes	reverse
T9907	3318-3331	NN	denotes	transcriptase
T9908	3332-3342	NNS	denotes	inhibitors
T9909	3343-3344	-LRB-	denotes	(
T9910	3344-3357	NNP	denotes	Supplementary
T9911	3358-3364	NNP	denotes	Figure
T9912	3365-3367	NNP	denotes	S2
T9913	3367-3368	-RRB-	denotes	)
T9914	3368-3369	.	denotes	.
T9915	3370-3375	DT	denotes	These
T9916	3376-3383	NNS	denotes	results
T9917	3384-3393	VBD	denotes	indicated
T9918	3394-3398	IN	denotes	that
T9919	3399-3401	IN	denotes	in
T9920	3402-3414	JJ	denotes	single-round
T9921	3415-3420	JJ	denotes	HIV-1
T9922	3421-3430	NN	denotes	infection
T9923	3431-3434	DT	denotes	the
T9924	3435-3440	NNP	denotes	NF-κB
T9925	3441-3451	NN	denotes	activation
T9926	3452-3461	RB	denotes	initially
T9927	3462-3472	VBD	denotes	correlated
T9928	3473-3477	IN	denotes	with
T9929	3478-3481	DT	denotes	the
T9930	3482-3485	NNP	denotes	IKK
T9931	3486-3496	NN	denotes	activation
T9932	3497-3500	CC	denotes	and
T9933	3501-3506	JJ	denotes	IκB-α
T9934	3507-3518	NN	denotes	degradation
T9935	3519-3532	RB	denotes	independently
T9936	3533-3535	IN	denotes	of
T9937	3536-3539	DT	denotes	the
T9938	3540-3548	NN	denotes	presence
T9939	3549-3551	IN	denotes	of
T9940	3552-3555	NNP	denotes	Tat
T9941	3555-3556	,	denotes	,
T9942	3557-3560	CC	denotes	and
T9943	3561-3563	PRP	denotes	it
T9944	3564-3567	VBD	denotes	was
T9945	3568-3572	VBN	denotes	kept
T9946	3573-3581	VBN	denotes	elevated
T9947	3582-3584	IN	denotes	in
T9948	3585-3593	NN	denotes	presence
T9949	3594-3596	IN	denotes	of
T9950	3597-3600	NNP	denotes	Tat
T9951	3601-3610	VBG	denotes	following
T9952	3611-3614	DT	denotes	the
T9953	3615-3620	NN	denotes	decay
T9954	3621-3623	IN	denotes	of
T9955	3624-3627	DT	denotes	the
T9956	3628-3631	NNP	denotes	IKK
T9957	3632-3640	NN	denotes	activity
T9958	3641-3644	CC	denotes	and
T9959	3645-3648	JJ	denotes	new
T9960	3649-3658	NN	denotes	synthesis
T9961	3659-3661	IN	denotes	of
T9962	3662-3667	NNP	denotes	IκB-α
T9963	3667-3668	.	denotes	.
T9964	3669-3673	JJ	denotes	Next
T9965	3673-3674	,	denotes	,
T9966	3675-3677	PRP	denotes	we
T9967	3678-3684	VBD	denotes	tested
T9968	3685-3688	DT	denotes	the
T9969	3689-3695	JJ	denotes	single
T9970	3696-3702	NN	denotes	action
T9971	3703-3705	IN	denotes	of
T9972	3706-3709	NNP	denotes	Tat
T9973	3710-3712	IN	denotes	on
T9974	3713-3716	DT	denotes	the
T9975	3717-3724	NN	denotes	kinetic
T9976	3725-3727	IN	denotes	of
T9977	3728-3733	JJ	denotes	NF-κB
T9978	3734-3742	NN	denotes	activity
T9979	3743-3750	VBN	denotes	induced
T9980	3751-3753	IN	denotes	by
T9981	3754-3757	NNP	denotes	PMA
T9982	3757-3758	.	denotes	.
T9983	3759-3761	TO	denotes	To
T9984	3762-3766	DT	denotes	this
T9985	3767-3770	NN	denotes	end
T9986	3770-3771	,	denotes	,
T9987	3772-3776	NNP	denotes	HeLa
T9988	3777-3782	NNS	denotes	cells
T9989	3783-3787	VBD	denotes	were
T9990	3788-3799	VBN	denotes	transfected
T9991	3800-3804	IN	denotes	with
T9992	3805-3817	NN	denotes	p3x-FLAG-Tat
T9993	3817-3818	,	denotes	,
T9994	3819-3821	CC	denotes	or
T9995	3822-3827	JJ	denotes	empty
T9996	3828-3834	NN	denotes	vector
T9997	3834-3835	,	denotes	,
T9998	3836-3846	VBN	denotes	stimulated
T9999	3847-3851	IN	denotes	with
T10000	3852-3855	NNP	denotes	PMA
T10001	3856-3859	IN	denotes	for
T10002	3860-3861	CD	denotes	5
T10003	3862-3865	NN	denotes	min
T10004	3865-3866	,	denotes	,
T10005	3867-3870	CC	denotes	and
T10006	3871-3882	RB	denotes	extensively
T10007	3883-3889	VBD	denotes	washed
T10008	3890-3892	TO	denotes	to
T10009	3893-3898	VB	denotes	avoid
T10010	3899-3902	DT	denotes	the
T10011	3903-3914	JJ	denotes	oscillatory
T10012	3915-3922	NN	denotes	kinetic
T10013	3923-3925	IN	denotes	of
T10014	3926-3931	JJ	denotes	NF-κB
T10015	3932-3942	NN	denotes	activation
T10016	3943-3944	-LRB-	denotes	(
T10017	3944-3949	CD	denotes	17,18
T10018	3949-3950	-RRB-	denotes	)
T10019	3950-3951	.	denotes	.
T10020	3952-3954	IN	denotes	In
T10021	3955-3968	JJ	denotes	un-stimulated
T10022	3969-3974	NNS	denotes	cells
T10023	3974-3975	,	denotes	,
T10024	3976-3979	DT	denotes	the
T10025	3980-3983	CD	denotes	p65
T10026	3984-3987	NNP	denotes	DNA
T10027	3988-3995	JJ	denotes	binding
T10028	3996-3997	-LRB-	denotes	(
T10029	3997-4003	NN	denotes	Figure
T10030	4004-4006	NN	denotes	2A
T10031	4006-4007	-RRB-	denotes	)
T10032	4008-4011	CC	denotes	and
T10033	4012-4019	JJ	denotes	nuclear
T10034	4020-4023	NNS	denotes	p65
T10035	4024-4025	-LRB-	denotes	(
T10036	4025-4031	NN	denotes	Figure
T10037	4032-4034	NN	denotes	2B
T10038	4034-4035	,	denotes	,
T10039	4036-4043	NN	denotes	nucleus
T10040	4043-4044	-RRB-	denotes	)
T10041	4045-4049	VBD	denotes	were
T10042	4050-4058	RB	denotes	slightly
T10043	4059-4067	VBN	denotes	enhanced
T10044	4068-4070	IN	denotes	by
T10045	4071-4074	NNP	denotes	Tat
T10046	4074-4075	,	denotes	,
T10047	4076-4081	IN	denotes	while
T10048	4082-4085	DT	denotes	the
T10049	4086-4091	JJ	denotes	IκB-α
T10050	4092-4099	NN	denotes	content
T10051	4100-4103	VBD	denotes	was
T10052	4104-4107	RB	denotes	not
T10053	4108-4121	RB	denotes	significantly
T10054	4122-4130	JJ	denotes	affected
T10055	4131-4132	-LRB-	denotes	(
T10056	4132-4138	NN	denotes	Figure
T10057	4139-4141	NN	denotes	2B
T10058	4141-4142	,	denotes	,
T10059	4143-4150	NN	denotes	cytosol
T10060	4150-4151	-RRB-	denotes	)
T10061	4151-4152	.	denotes	.
T10062	4153-4162	JJ	denotes	Transient
T10063	4163-4174	NN	denotes	stimulation
T10064	4175-4179	IN	denotes	with
T10065	4180-4183	NNP	denotes	PMA
T10066	4184-4193	VBD	denotes	increased
T10067	4194-4197	DT	denotes	the
T10068	4198-4203	NNP	denotes	NF-κB
T10069	4204-4207	NNP	denotes	DNA
T10070	4208-4215	JJ	denotes	binding
T10071	4216-4224	NN	denotes	activity
T10072	4225-4228	CC	denotes	and
T10073	4229-4236	JJ	denotes	nuclear
T10074	4237-4240	NNS	denotes	p65
T10075	4241-4243	IN	denotes	at
T10076	4244-4245	CD	denotes	5
T10077	4246-4249	NN	denotes	min
T10078	4250-4257	VBG	denotes	peaking
T10079	4258-4260	IN	denotes	at
T10080	4261-4263	CD	denotes	60
T10081	4264-4267	CD	denotes	120
T10082	4268-4271	NN	denotes	min
T10083	4272-4285	RB	denotes	independently
T10084	4286-4288	IN	denotes	of
T10085	4289-4292	DT	denotes	the
T10086	4293-4301	NN	denotes	presence
T10087	4302-4304	IN	denotes	of
T10088	4305-4308	NNP	denotes	Tat
T10089	4309-4310	-LRB-	denotes	(
T10090	4310-4316	NNP	denotes	Figure
T10091	4317-4319	NNP	denotes	2A
T10092	4320-4323	CC	denotes	and
T10093	4324-4325	NNP	denotes	B
T10094	4325-4326	-RRB-	denotes	)
T10095	4326-4327	:	denotes	;
T10096	4328-4335	RB	denotes	however
T10097	4335-4336	,	denotes	,
T10098	4337-4342	IN	denotes	while
T10099	4343-4346	DT	denotes	the
T10100	4347-4350	CD	denotes	p65
T10101	4351-4354	NNP	denotes	DNA
T10102	4355-4362	JJ	denotes	binding
T10103	4363-4366	CC	denotes	and
T10104	4367-4374	JJ	denotes	nuclear
T10105	4375-4378	NNS	denotes	p65
T10106	4379-4388	VBD	denotes	persisted
T10107	4389-4397	VBN	denotes	elevated
T10108	4398-4400	IN	denotes	at
T10109	4401-4408	JJ	denotes	240-min
T10110	4409-4423	NN	denotes	post-treatment
T10111	4424-4426	IN	denotes	in
T10112	4427-4439	JJ	denotes	Tat-positive
T10113	4440-4445	NNS	denotes	cells
T10114	4445-4446	,	denotes	,
T10115	4447-4451	PRP	denotes	they
T10116	4452-4459	VBD	denotes	dropped
T10117	4460-4462	IN	denotes	in
T10118	4463-4475	JJ	denotes	Tat-negative
T10119	4476-4481	NNS	denotes	cells
T10120	4482-4483	-LRB-	denotes	(
T10121	4483-4489	NN	denotes	Figure
T10122	4490-4492	CD	denotes	2A
T10123	4493-4496	CC	denotes	and
T10124	4497-4498	NNP	denotes	B
T10125	4498-4499	-RRB-	denotes	)
T10126	4499-4500	.	denotes	.
T10127	4501-4503	IN	denotes	In
T10128	4504-4512	NN	denotes	addition
T10129	4512-4513	,	denotes	,
T10130	4514-4517	NNP	denotes	Tat
T10131	4518-4523	VBD	denotes	bound
T10132	4524-4526	TO	denotes	to
T10133	4527-4530	DT	denotes	the
T10134	4531-4536	JJ	denotes	NF-κB
T10135	4537-4552	NN	denotes	oligonucleotide
T10136	4553-4555	IN	denotes	in
T10137	4556-4569	JJ	denotes	un-stimulated
T10138	4570-4575	NNS	denotes	cells
T10139	4576-4579	CC	denotes	and
T10140	4580-4583	PRP$	denotes	its
T10141	4584-4591	JJ	denotes	binding
T10142	4592-4601	VBN	denotes	increased
T10143	4602-4611	VBG	denotes	following
T10144	4612-4615	NNP	denotes	PMA
T10145	4616-4627	NN	denotes	stimulation
T10146	4628-4629	-LRB-	denotes	(
T10147	4629-4635	NN	denotes	Figure
T10148	4636-4638	NN	denotes	2A
T10149	4638-4639	-RRB-	denotes	)
T10150	4639-4640	,	denotes	,
T10151	4641-4651	VBG	denotes	suggesting
T10152	4652-4656	IN	denotes	that
T10153	4657-4660	DT	denotes	the
T10154	4661-4666	JJ	denotes	viral
T10155	4667-4674	NN	denotes	protein
T10156	4675-4678	VBD	denotes	was
T10157	4679-4680	DT	denotes	a
T10158	4681-4690	NN	denotes	component
T10159	4691-4693	IN	denotes	of
T10160	4694-4697	DT	denotes	the
T10161	4698-4703	JJ	denotes	NF-κB
T10162	4704-4711	JJ	denotes	complex
T10163	4712-4717	VBN	denotes	bound
T10164	4718-4720	TO	denotes	to
T10165	4721-4724	NNP	denotes	DNA
T10166	4724-4725	.	denotes	.
T10167	4726-4737	NN	denotes	Degradation
T10168	4738-4740	IN	denotes	of
T10169	4741-4746	NNP	denotes	IκB-α
T10170	4747-4750	VBD	denotes	was
T10171	4751-4764	RB	denotes	progressively
T10172	4765-4772	VBN	denotes	induced
T10173	4773-4775	IN	denotes	at
T10174	4776-4777	CD	denotes	5
T10175	4778-4780	CD	denotes	30
T10176	4781-4784	NN	denotes	min
T10177	4784-4785	,	denotes	,
T10178	4786-4794	VBN	denotes	followed
T10179	4795-4797	IN	denotes	by
T10180	4798-4800	FW	denotes	de
T10181	4801-4805	FW	denotes	novo
T10182	4806-4815	NN	denotes	synthesis
T10183	4816-4818	IN	denotes	at
T10184	4819-4821	CD	denotes	60
T10185	4822-4825	NN	denotes	min
T10186	4826-4830	IN	denotes	with
T10187	4831-4835	JJ	denotes	full
T10188	4836-4849	NN	denotes	replenishment
T10189	4850-4852	IN	denotes	at
T10190	4853-4856	CD	denotes	240
T10191	4857-4860	NN	denotes	min
T10192	4861-4863	IN	denotes	in
T10193	4864-4868	DT	denotes	both
T10194	4869-4881	JJ	denotes	Tat-positive
T10195	4882-4885	CC	denotes	and
T10196	4886-4894	JJ	denotes	negative
T10197	4895-4900	NNS	denotes	cells
T10198	4901-4902	-LRB-	denotes	(
T10199	4902-4908	NN	denotes	Figure
T10200	4909-4911	NN	denotes	2B
T10201	4911-4912	,	denotes	,
T10202	4913-4920	NN	denotes	cytosol
T10203	4920-4921	-RRB-	denotes	)
T10204	4921-4922	.	denotes	.
T10205	4923-4935	RB	denotes	Consistently
T10206	4935-4936	,	denotes	,
T10207	4937-4940	DT	denotes	the
T10208	4941-4944	NNP	denotes	IKK
T10209	4945-4955	NN	denotes	activation
T10210	4956-4963	VBD	denotes	started
T10211	4964-4966	IN	denotes	at
T10212	4967-4968	CD	denotes	5
T10213	4969-4972	NN	denotes	min
T10214	4972-4973	,	denotes	,
T10215	4974-4977	CC	denotes	and
T10216	4978-4984	VBD	denotes	turned
T10217	4985-4988	RP	denotes	off
T10218	4989-4991	IN	denotes	at
T10219	4992-4994	CD	denotes	30
T10220	4995-4998	NN	denotes	min
T10221	4999-5012	RB	denotes	independently
T10222	5013-5015	IN	denotes	of
T10223	5016-5019	DT	denotes	the
T10224	5020-5028	NN	denotes	presence
T10225	5029-5031	IN	denotes	of
T10226	5032-5035	NNP	denotes	Tat
T10227	5036-5037	-LRB-	denotes	(
T10228	5037-5043	NNP	denotes	Figure
T10229	5044-5046	NNP	denotes	2C
T10230	5046-5047	-RRB-	denotes	)
T10231	5047-5048	.	denotes	.
T10232	5049-5054	DT	denotes	These
T10233	5055-5062	NNS	denotes	results
T10234	5063-5072	VBD	denotes	indicated
T10235	5073-5077	IN	denotes	that
T10236	5078-5081	NNP	denotes	Tat
T10237	5082-5090	VBD	denotes	enhanced
T10238	5091-5094	DT	denotes	the
T10239	5095-5100	JJ	denotes	NF-κB
T10240	5101-5109	NN	denotes	activity
T10241	5110-5112	IN	denotes	in
T10242	5113-5126	JJ	denotes	un-stimulated
T10243	5127-5130	CC	denotes	and
T10244	5131-5145	JJ	denotes	PMA-stimulated
T10245	5146-5151	NNS	denotes	cells
T10246	5152-5159	IN	denotes	without
T10247	5160-5169	VBG	denotes	affecting
T10248	5170-5173	DT	denotes	the
T10249	5174-5177	NNP	denotes	IKK
T10250	5178-5186	NN	denotes	activity
T10251	5187-5190	CC	denotes	and
T10252	5191-5196	JJ	denotes	IκB-α
T10253	5197-5204	NN	denotes	content
T10254	5204-5205	.	denotes	.
T10255	6822-6824	IN	denotes	As
T10256	6825-6827	PRP	denotes	we
T10257	6828-6838	RB	denotes	previously
T10258	6839-6844	VBD	denotes	found
T10259	6845-6849	IN	denotes	that
T10260	6850-6853	NNP	denotes	Tat
T10261	6854-6859	VBZ	denotes	binds
T10262	6860-6862	TO	denotes	to
T10263	6863-6866	DT	denotes	the
T10264	6867-6872	JJ	denotes	sixth
T10265	6873-6880	NN	denotes	ankyrin
T10266	6881-6883	IN	denotes	of
T10267	6884-6889	NNP	denotes	IκB-α
T10268	6890-6891	-LRB-	denotes	(
T10269	6891-6896	CD	denotes	50,51
T10270	6896-6897	-RRB-	denotes	)
T10271	6897-6898	,	denotes	,
T10272	6899-6901	PRP	denotes	we
T10273	6902-6908	VBD	denotes	tested
T10274	6909-6916	IN	denotes	whether
T10275	6917-6920	NNP	denotes	Tat
T10276	6921-6933	VBD	denotes	counteracted
T10277	6934-6937	DT	denotes	the
T10278	6938-6948	NN	denotes	generation
T10279	6949-6951	IN	denotes	of
T10280	6952-6955	DT	denotes	the
T10281	6956-6961	NNP	denotes	IκB-α
T10282	6961-6962	NNP	denotes	/
T10283	6962-6967	NNP	denotes	NF-κB
T10284	6968-6975	NN	denotes	complex
T10285	6975-6976	.	denotes	.
T10286	6977-6979	TO	denotes	To
T10287	6980-6984	DT	denotes	this
T10288	6985-6988	NN	denotes	end
T10289	6988-6989	,	denotes	,
T10290	6990-6993	DT	denotes	the
T10291	6994-7005	NN	denotes	association
T10292	7006-7008	IN	denotes	of
T10293	7009-7014	JJ	denotes	IκB-α
T10294	7015-7019	IN	denotes	with
T10295	7020-7023	CD	denotes	p65
T10296	7024-7027	VBD	denotes	was
T10297	7028-7036	VBN	denotes	analysed
T10298	7037-7039	IN	denotes	in
T10299	7040-7044	NN	denotes	vivo
T10300	7045-7047	IN	denotes	at
T10301	7048-7049	CD	denotes	0
T10302	7050-7053	CC	denotes	and
T10303	7054-7057	CD	denotes	240
T10304	7058-7061	NN	denotes	min
T10305	7062-7067	IN	denotes	after
T10306	7068-7079	JJ	denotes	short-pulse
T10307	7080-7083	NNP	denotes	PMA
T10308	7083-7084	,	denotes	,
T10309	7085-7090	WDT	denotes	which
T10310	7091-7103	VBD	denotes	corresponded
T10311	7104-7106	TO	denotes	to
T10312	7107-7110	DT	denotes	the
T10313	7111-7115	NN	denotes	time
T10314	7116-7118	IN	denotes	of
T10315	7119-7132	JJ	denotes	un-stimulated
T10316	7133-7142	NN	denotes	condition
T10317	7143-7146	CC	denotes	and
T10318	7147-7162	NN	denotes	post-activation
T10319	7163-7165	IN	denotes	de
T10320	7166-7170	NN	denotes	novo
T10321	7171-7180	NN	denotes	synthesis
T10322	7181-7183	IN	denotes	of
T10323	7184-7189	NNP	denotes	IκB-α
T10324	7189-7190	,	denotes	,
T10325	7191-7203	RB	denotes	respectively
T10326	7203-7204	.	denotes	.
T10327	7205-7226	NNP	denotes	Coimmunoprecipitation
T10328	7227-7229	IN	denotes	of
T10329	7230-7235	NNP	denotes	IκB-α
T10330	7236-7240	IN	denotes	with
T10331	7241-7244	CD	denotes	p65
T10332	7245-7248	VBD	denotes	was
T10333	7249-7257	VBN	denotes	detected
T10334	7258-7260	IN	denotes	at
T10335	7261-7262	CD	denotes	0
T10336	7262-7263	:	denotes	-
T10337	7264-7267	CC	denotes	and
T10338	7268-7275	JJ	denotes	240-min
T10339	7276-7290	NN	denotes	post-treatment
T10340	7291-7293	IN	denotes	in
T10341	7294-7306	JJ	denotes	Tat-negative
T10342	7307-7312	NNS	denotes	cells
T10343	7313-7314	-LRB-	denotes	(
T10344	7314-7320	NN	denotes	Figure
T10345	7321-7323	CD	denotes	2D
T10346	7323-7324	,	denotes	,
T10347	7325-7330	NNS	denotes	lanes
T10348	7331-7332	CD	denotes	1
T10349	7333-7336	CC	denotes	and
T10350	7337-7338	CD	denotes	3
T10351	7338-7339	-RRB-	denotes	)
T10352	7339-7340	,	denotes	,
T10353	7341-7344	CC	denotes	and
T10354	7345-7348	VBD	denotes	was
T10355	7349-7355	VBN	denotes	halved
T10356	7356-7358	IN	denotes	in
T10357	7359-7371	JJ	denotes	Tat-positive
T10358	7372-7377	NNS	denotes	cells
T10359	7377-7378	,	denotes	,
T10360	7379-7384	WRB	denotes	where
T10361	7385-7388	NNP	denotes	Tat
T10362	7389-7409	VBD	denotes	coimmunoprecipitated
T10363	7410-7414	IN	denotes	with
T10364	7415-7420	NNP	denotes	IκB-α
T10365	7421-7422	-LRB-	denotes	(
T10366	7422-7428	NNP	denotes	Figure
T10367	7429-7431	NNP	denotes	2D
T10368	7431-7432	,	denotes	,
T10369	7433-7438	NNS	denotes	lanes
T10370	7439-7440	CD	denotes	2
T10371	7441-7444	CC	denotes	and
T10372	7445-7446	CD	denotes	4
T10373	7446-7447	-RRB-	denotes	)
T10374	7447-7448	,	denotes	,
T10375	7449-7459	VBG	denotes	indicating
T10376	7460-7464	IN	denotes	that
T10377	7465-7468	NNP	denotes	Tat
T10378	7469-7477	VBD	denotes	competed
T10379	7478-7481	DT	denotes	the
T10380	7482-7487	JJ	denotes	IκB-α
T10381	7488-7495	JJ	denotes	binding
T10382	7496-7498	TO	denotes	to
T10383	7499-7502	NNS	denotes	p65
T10384	7502-7503	.	denotes	.
T23945	1071-1074	NNP	denotes	Tat
T23946	1075-1086	VBZ	denotes	counteracts
T23947	1087-1090	DT	denotes	the
T23948	1091-1106	JJ	denotes	post-activation
T23949	1107-1111	NN	denotes	turn
T23950	1112-1115	IN	denotes	off
T23951	1116-1118	IN	denotes	of
T23952	1119-1124	NN	denotes	NF-κB
T23953	1125-1127	IN	denotes	in
T23954	1128-1134	JJ	denotes	single
T23955	1135-1140	NN	denotes	round
T23956	1141-1156	NN	denotes	HIV-1-infection
T23957	1156-1157	.	denotes	.
T23958	1158-1164	NNP	denotes	Jurkat
T23959	1165-1170	NNS	denotes	cells
T23960	1171-1172	-LRB-	denotes	(
T23961	1172-1173	CD	denotes	5
T23962	1174-1175	RB	denotes	×
T23963	1176-1179	CD	denotes	107
T23964	1179-1180	-RRB-	denotes	)
T23965	1181-1185	VBD	denotes	were
T23966	1186-1197	VBN	denotes	transfected
T23967	1198-1202	IN	denotes	with
T23968	1203-1208	NNP	denotes	siRNA
T23969	1209-1212	NNP	denotes	Tat
T23970	1213-1215	CC	denotes	or
T23971	1216-1221	NNP	denotes	siRNA
T23972	1222-1229	NN	denotes	control
T23973	1230-1231	-LRB-	denotes	(
T23974	1231-1232	CD	denotes	2
T23975	1233-1237	NN	denotes	nmol
T23976	1237-1238	-RRB-	denotes	)
T23977	1238-1239	,	denotes	,
T23978	1240-1242	CC	denotes	or
T23979	1243-1247	VBD	denotes	left
T23980	1248-1261	JJ	denotes	untransfected
T23981	1261-1262	:	denotes	;
T23982	1263-1265	CD	denotes	24
T23983	1266-1267	NN	denotes	h
T23984	1268-1273	RB	denotes	later
T23985	1273-1274	,	denotes	,
T23986	1275-1280	NNS	denotes	cells
T23987	1281-1285	VBD	denotes	were
T23988	1286-1294	VBN	denotes	infected
T23989	1295-1299	IN	denotes	with
T23990	1300-1316	JJ	denotes	HXB2-pseudotyped
T23991	1317-1322	NN	denotes	NL4-3
T23992	1322-1323	.	denotes	.
T23993	1323-1328	NNP	denotes	Luc.R
T23994	1328-1329	NNP	denotes	−
T23995	1329-1330	NNP	denotes	E
T23996	1330-1331	VBD	denotes	−
T23997	1332-1339	NNS	denotes	virions
T23998	1340-1341	-LRB-	denotes	(
T23999	1341-1344	CD	denotes	500
T24000	1345-1347	NN	denotes	ng
T24001	1348-1350	IN	denotes	of
T24002	1351-1354	CD	denotes	p24
T24003	1354-1355	-RRB-	denotes	)
T24004	1356-1359	CC	denotes	and
T24005	1360-1369	VBD	denotes	harvested
T24006	1370-1372	IN	denotes	at
T24007	1373-1376	DT	denotes	the
T24008	1377-1386	JJ	denotes	indicated
T24009	1387-1391	NN	denotes	time
T24010	1391-1392	.	denotes	.
T24011	1393-1394	-LRB-	denotes	(
T24012	1394-1395	NNP	denotes	A
T24013	1395-1396	-RRB-	denotes	)
T24014	1397-1400	NNP	denotes	Tat
T24015	1401-1411	NN	denotes	expression
T24016	1412-1415	VBD	denotes	was
T24017	1416-1424	VBN	denotes	measured
T24018	1425-1427	IN	denotes	in
T24019	1428-1433	JJ	denotes	total
T24020	1434-1437	NNP	denotes	RNA
T24021	1438-1440	IN	denotes	by
T24022	1441-1450	JJ	denotes	real-time
T24023	1451-1454	NNP	denotes	PCR
T24024	1454-1455	,	denotes	,
T24025	1456-1461	IN	denotes	while
T24026	1462-1465	DT	denotes	the
T24027	1466-1476	NN	denotes	luciferase
T24028	1477-1485	NN	denotes	activity
T24029	1486-1489	VBD	denotes	was
T24030	1490-1498	VBN	denotes	measured
T24031	1499-1501	IN	denotes	in
T24032	1502-1507	JJ	denotes	whole
T24033	1508-1512	NN	denotes	cell
T24034	1513-1521	NNS	denotes	extracts
T24035	1521-1522	.	denotes	.
T24036	1523-1524	-LRB-	denotes	(
T24037	1524-1525	NNP	denotes	B
T24038	1525-1526	-RRB-	denotes	)
T24039	1527-1534	NNP	denotes	Nuclear
T24040	1535-1543	NNS	denotes	extracts
T24041	1544-1545	-LRB-	denotes	(
T24042	1545-1547	CD	denotes	10
T24043	1548-1550	NN	denotes	µg
T24044	1550-1551	-RRB-	denotes	)
T24045	1552-1556	VBD	denotes	were
T24046	1557-1565	VBN	denotes	analysed
T24047	1566-1569	IN	denotes	for
T24048	1570-1573	DT	denotes	the
T24049	1574-1577	NNS	denotes	p65
T24050	1578-1581	CC	denotes	and
T24051	1582-1585	JJ	denotes	p50
T24052	1586-1593	JJ	denotes	binding
T24053	1594-1596	TO	denotes	to
T24054	1597-1600	DT	denotes	the
T24055	1601-1606	NNP	denotes	NF-κB
T24056	1607-1622	JJ	denotes	double-stranded
T24057	1623-1638	NN	denotes	oligonucleotide
T24058	1639-1644	VBG	denotes	using
T24059	1645-1648	DT	denotes	the
T24060	1649-1654	NNP	denotes	NF-κB
T24061	1655-1668	NNP	denotes	Transcription
T24062	1669-1675	NNP	denotes	Factor
T24063	1676-1681	NNP	denotes	ELISA
T24064	1682-1687	NNP	denotes	Assay
T24065	1688-1691	NN	denotes	kit
T24066	1692-1693	-LRB-	denotes	(
T24067	1693-1699	NNP	denotes	Cayman
T24068	1699-1700	-RRB-	denotes	)
T24069	1700-1701	.	denotes	.
T24070	1702-1703	-LRB-	denotes	(
T24071	1703-1704	$	denotes	C
T24072	1704-1705	-RRB-	denotes	)
T24073	1706-1709	DT	denotes	The
T24074	1710-1720	NN	denotes	expression
T24075	1721-1723	IN	denotes	of
T24076	1724-1727	CD	denotes	p65
T24077	1728-1731	CC	denotes	and
T24078	1732-1737	NNP	denotes	IκB-α
T24079	1738-1741	VBD	denotes	was
T24080	1742-1750	VBN	denotes	analysed
T24081	1751-1753	IN	denotes	by
T24082	1754-1756	CD	denotes	12
T24083	1756-1757	NN	denotes	%
T24084	1758-1761	NNP	denotes	SDS
T24085	1762-1766	NNP	denotes	PAGE
T24086	1767-1770	CC	denotes	and
T24087	1771-1778	JJ	denotes	western
T24088	1779-1787	VBG	denotes	blotting
T24089	1788-1790	IN	denotes	of
T24090	1791-1798	JJ	denotes	nuclear
T24091	1799-1801	CC	denotes	or
T24092	1802-1811	JJ	denotes	cytosolic
T24093	1812-1820	NNS	denotes	extracts
T24094	1821-1822	-LRB-	denotes	(
T24095	1822-1824	CD	denotes	20
T24096	1825-1827	NN	denotes	µg
T24097	1827-1828	-RRB-	denotes	)
T24098	1829-1834	VBG	denotes	using
T24099	1835-1843	JJ	denotes	anti-p65
T24100	1844-1847	CC	denotes	and
T24101	1848-1858	JJ	denotes	anti-IκB-α
T24102	1859-1869	NNS	denotes	antibodies
T24103	1869-1870	.	denotes	.
T24104	1871-1878	NNP	denotes	Histone
T24105	1879-1881	NNP	denotes	H1
T24114	1939-1941	IN	denotes	as
T24115	1942-1949	NNS	denotes	markers
T24116	1950-1952	IN	denotes	of
T24117	1953-1960	JJ	denotes	nuclear
T24118	1961-1964	CC	denotes	and
T24119	1965-1974	JJ	denotes	cytosolic
T24120	1975-1983	NNS	denotes	extracts
T24121	1983-1984	,	denotes	,
T24122	1985-1997	RB	denotes	respectively
T24123	1997-1998	.	denotes	.
T24124	1999-2011	NNP	denotes	Densitometry
T24125	2012-2018	NNS	denotes	values
T24126	2019-2020	-LRB-	denotes	(
T24127	2020-2021	NNP	denotes	D
T24128	2021-2022	-RRB-	denotes	)
T24129	2023-2025	IN	denotes	of
T24130	2026-2029	DT	denotes	the
T24131	2030-2035	NNS	denotes	bands
T24132	2036-2040	VBD	denotes	were
T24133	2041-2050	VBN	denotes	expressed
T24134	2051-2053	IN	denotes	as
T24135	2054-2058	VBP	denotes	fold
T24136	2059-2067	NN	denotes	increase
T24137	2068-2073	IN	denotes	above
T24138	2074-2077	DT	denotes	the
T24139	2078-2085	NN	denotes	control
T24140	2086-2087	-LRB-	denotes	(
T24141	2087-2091	JJ	denotes	mock
T24142	2091-2092	-RRB-	denotes	)
T24143	2092-2093	.	denotes	.
T24144	2094-2095	-LRB-	denotes	(
T24145	2095-2096	NNP	denotes	D
T24146	2096-2097	-RRB-	denotes	)
T24147	2098-2101	NNP	denotes	IKK
T24148	2102-2110	NN	denotes	activity
T24149	2111-2114	VBD	denotes	was
T24150	2115-2123	VBN	denotes	measured
T24151	2124-2126	IN	denotes	in
T24152	2127-2136	JJ	denotes	cytosolic
T24153	2137-2145	NNS	denotes	extracts
T24154	2146-2147	-LRB-	denotes	(
T24155	2147-2150	CD	denotes	100
T24156	2151-2153	NN	denotes	µg
T24157	2153-2154	-RRB-	denotes	)
T24158	2155-2157	IN	denotes	by
T24159	2158-2163	VBG	denotes	using
T24160	2164-2170	NNP	denotes	HTScan
T24161	2171-2174	NNP	denotes	IKK
T24162	2175-2181	NNP	denotes	Kinase
T24163	2182-2187	NNP	denotes	Assay
T24164	2188-2189	-LRB-	denotes	(
T24165	2189-2193	NNP	denotes	Cell
T24166	2194-2203	NNP	denotes	Signaling
T24167	2204-2214	NNP	denotes	Technology
T24168	2214-2215	-RRB-	denotes	)
T24169	2215-2216	.	denotes	.
T24170	2217-2223	NNS	denotes	Values
T24171	2224-2225	-LRB-	denotes	(
T24172	2225-2229	JJ	denotes	mean
T24173	2230-2231	NN	denotes	±
T24174	2232-2234	NNP	denotes	SE
T24175	2234-2235	,	denotes	,
T24176	2236-2237	JJ	denotes	n
T24177	2238-2239	SYM	denotes	=
T24178	2240-2241	LS	denotes	3
T24179	2241-2242	-RRB-	denotes	)
T24180	2243-2246	VBP	denotes	are
T24181	2247-2252	VBN	denotes	shown
T24182	2252-2253	.	denotes	.
T24672	5216-5219	NNP	denotes	Tat
T24673	5220-5228	VBZ	denotes	enhances
T24674	5229-5232	DT	denotes	the
T24675	5233-5238	JJ	denotes	NF-κB
T24676	5239-5247	NN	denotes	activity
T24677	5248-5257	VBG	denotes	following
T24678	5258-5261	NNP	denotes	PMA
T24679	5262-5273	NN	denotes	stimulation
T24680	5274-5276	IN	denotes	by
T24681	5277-5288	VBG	denotes	associating
T24682	5289-5293	IN	denotes	with
T24683	5294-5299	NNP	denotes	IκB-α
T24684	5300-5303	CC	denotes	and
T24685	5304-5317	VBG	denotes	counteracting
T24686	5318-5321	DT	denotes	the
T24687	5322-5327	JJ	denotes	NF-κB
T24688	5328-5338	NN	denotes	repression
T24689	5338-5339	.	denotes	.
T24690	5340-5344	NNP	denotes	HeLa
T24691	5345-5350	NNS	denotes	cells
T24692	5351-5352	-LRB-	denotes	(
T24693	5352-5353	CD	denotes	5
T24694	5354-5355	CD	denotes	×
T24695	5356-5359	CD	denotes	106
T24696	5359-5360	-RRB-	denotes	)
T24697	5361-5365	VBD	denotes	were
T24698	5366-5377	VBN	denotes	transfected
T24699	5378-5382	IN	denotes	with
T24700	5383-5394	NN	denotes	p3xFLAG-Tat
T24701	5395-5397	CC	denotes	or
T24702	5398-5405	JJ	denotes	p3xFLAG
T24703	5406-5411	JJ	denotes	empty
T24704	5412-5418	NN	denotes	vector
T24705	5419-5420	-LRB-	denotes	(
T24706	5420-5421	CD	denotes	5
T24707	5422-5424	NN	denotes	µg
T24708	5424-5425	-RRB-	denotes	)
T24709	5425-5426	,	denotes	,
T24710	5427-5430	CC	denotes	and
T24711	5431-5433	CD	denotes	48
T24712	5434-5435	NN	denotes	h
T24713	5436-5441	RB	denotes	later
T24714	5442-5446	VBD	denotes	were
T24715	5447-5457	VBN	denotes	stimulated
T24716	5458-5462	IN	denotes	with
T24717	5463-5466	NNP	denotes	PMA
T24718	5467-5468	-LRB-	denotes	(
T24719	5468-5469	CD	denotes	5
T24720	5470-5473	NN	denotes	min
T24721	5473-5474	,	denotes	,
T24722	5475-5477	CD	denotes	20
T24723	5478-5483	NN	denotes	ng/ml
T24724	5483-5484	-RRB-	denotes	)
T24725	5485-5487	CC	denotes	or
T24726	5488-5492	VBD	denotes	left
T24727	5493-5505	JJ	denotes	unstimulated
T24728	5505-5506	,	denotes	,
T24729	5507-5513	VBD	denotes	washed
T24730	5514-5519	RB	denotes	twice
T24731	5520-5524	IN	denotes	with
T24732	5525-5529	NNP	denotes	DMEM
T24733	5530-5533	CC	denotes	and
T24734	5534-5543	VBN	denotes	harvested
T24735	5544-5546	IN	denotes	at
T24736	5547-5550	DT	denotes	the
T24737	5551-5560	JJ	denotes	indicated
T24738	5561-5566	NNS	denotes	times
T24739	5566-5567	.	denotes	.
T24740	5568-5575	NNP	denotes	Nuclear
T24741	5576-5579	CC	denotes	and
T24742	5580-5589	JJ	denotes	cytosolic
T24743	5590-5598	NNS	denotes	extracts
T24744	5599-5603	VBD	denotes	were
T24745	5604-5612	VBN	denotes	prepared
T24746	5613-5616	IN	denotes	for
T24747	5617-5624	JJ	denotes	further
T24748	5625-5633	NN	denotes	analysis
T24749	5633-5634	.	denotes	.
T24750	5635-5636	-LRB-	denotes	(
T24751	5636-5637	NNP	denotes	A
T24752	5637-5638	-RRB-	denotes	)
T24753	5639-5646	NNP	denotes	Nuclear
T24754	5647-5655	NNS	denotes	extracts
T24755	5656-5660	VBD	denotes	were
T24756	5661-5669	VBN	denotes	analysed
T24757	5670-5673	IN	denotes	for
T24758	5674-5677	DT	denotes	the
T24759	5678-5681	NNS	denotes	p65
T24760	5681-5682	,	denotes	,
T24761	5683-5686	JJ	denotes	p50
T24762	5687-5690	CC	denotes	and
T24763	5691-5699	JJ	denotes	FLAG-Tat
T24764	5700-5707	JJ	denotes	binding
T24765	5708-5710	TO	denotes	to
T24766	5711-5714	DT	denotes	the
T24767	5715-5720	NNP	denotes	NF-κB
T24768	5721-5736	JJ	denotes	double-stranded
T24769	5737-5752	NN	denotes	oligonucleotide
T24770	5753-5758	VBG	denotes	using
T24771	5759-5762	DT	denotes	the
T24772	5763-5768	NNP	denotes	NF-κB
T24773	5769-5782	NNP	denotes	Transcription
T24774	5783-5789	NNP	denotes	Factor
T24775	5790-5795	NNP	denotes	ELISA
T24776	5796-5801	NN	denotes	assay
T24777	5802-5805	NN	denotes	kit
T24778	5806-5807	-LRB-	denotes	(
T24779	5807-5813	NNP	denotes	Cayman
T24780	5813-5814	-RRB-	denotes	)
T24781	5814-5815	.	denotes	.
T24782	5816-5817	-LRB-	denotes	(
T24783	5817-5818	NNP	denotes	B
T24784	5818-5819	-RRB-	denotes	)
T24785	5820-5827	NNP	denotes	Nuclear
T24786	5828-5831	CC	denotes	and
T24787	5832-5841	JJ	denotes	cytosolic
T24788	5842-5850	NNS	denotes	extracts
T24789	5851-5852	-LRB-	denotes	(
T24790	5852-5854	CD	denotes	20
T24791	5855-5857	NN	denotes	µg
T24792	5857-5858	-RRB-	denotes	)
T24793	5859-5863	VBD	denotes	were
T24794	5864-5873	VBN	denotes	separated
T24795	5874-5876	IN	denotes	by
T24796	5877-5879	CD	denotes	12
T24797	5879-5880	NN	denotes	%
T24798	5881-5884	NNP	denotes	SDS
T24799	5885-5889	NNP	denotes	PAGE
T24800	5890-5893	CC	denotes	and
T24801	5894-5902	VBN	denotes	analysed
T24802	5903-5905	IN	denotes	by
T24803	5906-5913	JJ	denotes	western
T24804	5914-5922	VBG	denotes	blotting
T24805	5923-5928	VBG	denotes	using
T24806	5929-5932	DT	denotes	the
T24807	5933-5941	JJ	denotes	anti-p65
T24808	5941-5942	,	denotes	,
T24809	5943-5952	JJ	denotes	anti-FLAG
T24810	5952-5953	,	denotes	,
T24811	5954-5966	JJ	denotes	anti-Histone
T24812	5967-5969	NN	denotes	H1
T24813	5969-5970	,	denotes	,
T24814	5971-5981	JJ	denotes	anti-IκB-α
T24815	5982-5985	CC	denotes	and
T24816	5986-6004	JJ	denotes	anti-Hexokinase-II
T24817	6005-6015	NNS	denotes	antibodies
T24818	6015-6016	.	denotes	.
T24819	6017-6029	NNP	denotes	Densitometry
T24820	6030-6036	NNS	denotes	values
T24821	6037-6038	-LRB-	denotes	(
T24822	6038-6039	NNP	denotes	D
T24823	6039-6040	-RRB-	denotes	)
T24824	6041-6043	IN	denotes	of
T24825	6044-6047	DT	denotes	the
T24826	6048-6053	NNS	denotes	bands
T24827	6054-6058	VBD	denotes	were
T24828	6059-6068	VBN	denotes	expressed
T24829	6069-6071	IN	denotes	as
T24830	6072-6076	VBP	denotes	fold
T24831	6077-6085	NN	denotes	increase
T24832	6086-6091	IN	denotes	above
T24833	6092-6095	DT	denotes	the
T24834	6096-6103	NN	denotes	control
T24835	6104-6105	-LRB-	denotes	(
T24836	6105-6109	NN	denotes	lane
T24837	6110-6111	CD	denotes	1
T24838	6111-6112	-RRB-	denotes	)
T24839	6112-6113	.	denotes	.
T24840	6114-6115	-LRB-	denotes	(
T24841	6115-6116	NNP	denotes	C
T24842	6116-6117	-RRB-	denotes	)
T24843	6118-6121	NNP	denotes	IKK
T24844	6122-6130	NN	denotes	activity
T24845	6131-6134	VBD	denotes	was
T24846	6135-6143	VBN	denotes	measured
T24847	6144-6146	IN	denotes	in
T24848	6147-6156	JJ	denotes	cytosolic
T24849	6157-6165	NNS	denotes	extracts
T24850	6166-6167	-LRB-	denotes	(
T24851	6167-6170	CD	denotes	100
T24852	6171-6173	NN	denotes	µg
T24853	6173-6174	-RRB-	denotes	)
T24854	6175-6177	IN	denotes	by
T24855	6178-6183	VBG	denotes	using
T24856	6184-6190	NNP	denotes	HTScan
T24857	6191-6194	NNP	denotes	IKK
T24858	6195-6201	NN	denotes	kinase
T24859	6202-6207	NN	denotes	assay
T24860	6208-6209	-LRB-	denotes	(
T24861	6209-6213	NNP	denotes	Cell
T24862	6214-6223	NNP	denotes	Signaling
T24863	6224-6234	NNP	denotes	Technology
T24864	6234-6235	-RRB-	denotes	)
T24865	6235-6236	.	denotes	.
T24866	6237-6238	-LRB-	denotes	(
T24867	6238-6239	NNP	denotes	D
T24868	6239-6240	-RRB-	denotes	)
T24869	6241-6245	NNP	denotes	HeLa
T24870	6246-6251	NNS	denotes	cells
T24871	6252-6253	-LRB-	denotes	(
T24872	6253-6254	CD	denotes	5
T24873	6255-6256	CD	denotes	×
T24874	6257-6260	CD	denotes	106
T24875	6260-6261	-RRB-	denotes	)
T24876	6262-6266	VBD	denotes	were
T24877	6267-6278	VBN	denotes	transfected
T24878	6279-6283	IN	denotes	with
T24879	6284-6286	CC	denotes	or
T24880	6287-6294	IN	denotes	without
T24881	6295-6306	NN	denotes	p3xFLAG-Tat
T24882	6307-6308	-LRB-	denotes	(
T24883	6308-6309	CD	denotes	5
T24884	6310-6312	NN	denotes	µg
T24885	6312-6313	-RRB-	denotes	)
T24886	6313-6314	:	denotes	;
T24887	6315-6317	CD	denotes	48
T24888	6318-6319	NN	denotes	h
T24889	6320-6325	RB	denotes	later
T24890	6325-6326	,	denotes	,
T24891	6327-6332	NNS	denotes	cells
T24892	6333-6337	VBD	denotes	were
T24893	6338-6348	VBN	denotes	stimulated
T24894	6349-6353	IN	denotes	with
T24895	6354-6357	NNP	denotes	PMA
T24896	6358-6359	-LRB-	denotes	(
T24897	6359-6360	CD	denotes	5
T24898	6361-6364	NN	denotes	min
T24899	6364-6365	,	denotes	,
T24900	6366-6368	CD	denotes	20
T24901	6369-6374	NN	denotes	ng/ml
T24902	6374-6375	-RRB-	denotes	)
T24903	6376-6378	CC	denotes	or
T24904	6379-6383	VBD	denotes	left
T24905	6384-6396	JJ	denotes	unstimulated
T24906	6396-6397	,	denotes	,
T24907	6398-6404	VBD	denotes	washed
T24908	6405-6410	RB	denotes	twice
T24909	6411-6415	IN	denotes	with
T24910	6416-6420	NNP	denotes	DMEM
T24911	6420-6421	,	denotes	,
T24912	6422-6425	CC	denotes	and
T24913	6426-6435	VBN	denotes	harvested
T24914	6436-6441	IN	denotes	after
T24915	6442-6445	CD	denotes	240
T24916	6446-6449	NN	denotes	min
T24917	6449-6450	.	denotes	.
T24918	6451-6456	JJ	denotes	Whole
T24919	6457-6461	NN	denotes	cell
T24920	6462-6470	NNS	denotes	extracts
T24921	6471-6472	-LRB-	denotes	(
T24922	6472-6473	CD	denotes	1
T24923	6474-6476	NN	denotes	mg
T24924	6476-6477	-RRB-	denotes	)
T24925	6478-6482	VBD	denotes	were
T24926	6483-6501	VBN	denotes	immunoprecipitated
T24927	6502-6506	IN	denotes	with
T24928	6507-6514	NN	denotes	protein
T24929	6515-6534	JJ	denotes	G-Sepharose-coupled
T24930	6535-6545	JJ	denotes	anti-IκB-α
T24931	6546-6554	NN	denotes	antibody
T24932	6554-6555	.	denotes	.
T24933	6556-6571	NNS	denotes	Immunocomplexes
T24934	6572-6576	VBD	denotes	were
T24935	6577-6586	VBN	denotes	separated
T24936	6587-6589	IN	denotes	by
T24937	6590-6592	CD	denotes	12
T24938	6592-6593	NN	denotes	%
T24939	6594-6597	NNP	denotes	SDS
T24940	6598-6602	NNP	denotes	PAGE
T24941	6603-6606	CC	denotes	and
T24942	6607-6615	VBN	denotes	analysed
T24943	6616-6618	IN	denotes	by
T24944	6619-6626	JJ	denotes	western
T24945	6627-6635	VBG	denotes	blotting
T24946	6636-6640	IN	denotes	with
T24947	6641-6649	JJ	denotes	anti-p65
T24948	6649-6650	,	denotes	,
T24949	6651-6660	JJ	denotes	anti-FLAG
T24950	6661-6664	CC	denotes	and
T24951	6665-6675	JJ	denotes	anti-IκB-α
T24952	6676-6686	NNS	denotes	antibodies
T24953	6686-6687	.	denotes	.
T24954	6688-6700	NNP	denotes	Densitometry
T24955	6701-6707	NNS	denotes	values
T24956	6708-6709	-LRB-	denotes	(
T24957	6709-6710	NNP	denotes	D
T24958	6710-6711	-RRB-	denotes	)
T24959	6712-6714	IN	denotes	of
T24960	6715-6718	DT	denotes	the
T24961	6719-6724	NNS	denotes	bands
T24962	6725-6729	VBD	denotes	were
T24963	6730-6739	VBN	denotes	expressed
T24964	6740-6742	IN	denotes	as
T24965	6743-6747	VBP	denotes	fold
T24966	6748-6756	NN	denotes	increase
T24967	6757-6762	IN	denotes	above
T24968	6763-6766	DT	denotes	the
T24969	6767-6774	NN	denotes	control
T24970	6775-6776	-LRB-	denotes	(
T24971	6776-6780	NN	denotes	lane
T24972	6781-6782	CD	denotes	1
T24973	6782-6783	-RRB-	denotes	)
T24974	6783-6784	.	denotes	.
T24975	6785-6791	NNS	denotes	Values
T24976	6792-6793	-LRB-	denotes	(
T24977	6793-6797	JJ	denotes	mean
T24978	6798-6799	NN	denotes	±
T24979	6800-6802	NNP	denotes	SE
T24980	6802-6803	,	denotes	,
T24981	6804-6805	JJ	denotes	n
T24982	6806-6807	SYM	denotes	=
T24983	6808-6809	LS	denotes	3
T24984	6809-6810	-RRB-	denotes	)
T24985	6811-6814	VBP	denotes	are
T24986	6815-6820	VBN	denotes	shown
T24987	6820-6821	.	denotes	.
T24106	1882-1885	CC	denotes	and
T24107	1886-1896	NNP	denotes	Hexokinase
T24108	1897-1899	NNP	denotes	II
T24109	1900-1904	VBD	denotes	were
T24110	1905-1913	VBN	denotes	detected
T24111	1914-1918	IN	denotes	with
T24112	1919-1927	JJ	denotes	specific
T24113	1928-1938	NNS	denotes	antibodies
R7872	T9532	T9533	nsubj	Tat,enhances
R7873	T9533	T9533	ROOT	enhances,enhances
R7874	T9534	T9536	det	the,activity
R7875	T9535	T9536	amod	NF-κB,activity
R7876	T9536	T9533	dobj	activity,enhances
R7877	T9537	T9536	prep	by,activity
R7878	T9538	T9537	pcomp	hijacking,by
R7879	T9539	T9538	dobj	IκB-α,hijacking
R7880	T9540	T9538	cc	and,hijacking
R7881	T9541	T9538	conj	inhibiting,hijacking
R7882	T9542	T9544	det	the,turn
R7883	T9543	T9544	nsubj	post-activation,turn
R7884	T9544	T9541	dobj	turn,inhibiting
R7885	T9545	T9544	prt	off,turn
R7886	T9546	T9545	prep	of,off
R7887	T9547	T9546	pobj	NF-κB,of
R7888	T9548	T9549	nsubj	We,analysed
R7889	T9549	T9536	relcl	analysed,activity
R7890	T9550	T9551	det	the,kinetic
R7891	T9551	T9549	dobj	kinetic,analysed
R7892	T9552	T9551	prep	of,kinetic
R7893	T9553	T9554	amod	NF-κB,activation
R7894	T9554	T9552	pobj	activation,of
R7895	T9555	T9554	prep	in,activation
R7896	T9556	T9559	amod	single,infection
R7897	T9557	T9559	compound	round,infection
R7898	T9558	T9559	compound	HIV-1,infection
R7899	T9559	T9555	pobj	infection,in
R7900	T9560	T9549	prep	by,analysed
R7901	T9561	T9560	pcomp	modulating,by
R7902	T9562	T9563	det	the,expression
R7903	T9563	T9561	dobj	expression,modulating
R7904	T9564	T9563	prep	of,expression
R7905	T9565	T9564	pobj	Tat,of
R7906	T9566	T9561	prep	with,modulating
R7907	T9567	T9568	compound	RNA,interference
R7908	T9568	T9566	pobj	interference,with
R7909	T9569	T9533	punct	.,enhances
R7910	T9570	T9571	compound	Jurkat,cells
R7911	T9571	T9573	nsubjpass	cells,transfected
R7912	T9572	T9573	auxpass	were,transfected
R7913	T9573	T9573	ROOT	transfected,transfected
R7914	T9574	T9573	prep	with,transfected
R7915	T9575	T9576	compound	siRNA,Tat
R7916	T9576	T9574	pobj	Tat,with
R7917	T9577	T9573	punct	",",transfected
R7918	T9578	T9579	compound	siRNA,control
R7919	T9579	T9573	conj	control,transfected
R7920	T9580	T9579	punct	",",control
R7921	T9581	T9579	cc	or,control
R7922	T9582	T9573	conj	left,transfected
R7923	T9583	T9582	oprd	untransfected,left
R7924	T9584	T9582	punct	",",left
R7925	T9585	T9582	cc	and,left
R7926	T9586	T9589	nummod	24,cells
R7927	T9587	T9589	nmod	h,cells
R7928	T9588	T9589	amod	later,cells
R7929	T9589	T9591	nsubjpass	cells,infected
R7930	T9590	T9591	auxpass	were,infected
R7931	T9591	T9582	conj	infected,left
R7932	T9592	T9591	prep	with,infected
R7933	T9593	T9595	nummod	HXB2,NL4-3
R7934	T9594	T9595	amod	Env-pseudotyped,NL4-3
R7935	T9595	T9592	pobj	NL4-3,with
R7936	T9596	T9591	punct	.,infected
R7937	T9597	T9601	compound	Luc.R,virions
R7938	T9598	T9601	compound	−,virions
R7939	T9599	T9600	compound	E,−
R7940	T9600	T9601	compound	−,virions
R7941	T9601	T9601	ROOT	virions,virions
R7942	T9602	T9601	punct	.,virions
R7943	T9603	T9612	prep	By,was
R7944	T9604	T9605	amod	cytofluorimetric,analysis
R7945	T9605	T9603	pobj	analysis,By
R7946	T9606	T9612	punct	",",was
R7947	T9607	T9612	advmod	∼,was
R7948	T9608	T9609	nummod	40,%
R7949	T9609	T9612	nsubj	%,was
R7950	T9610	T9609	prep	of,%
R7951	T9611	T9610	pobj	cells,of
R7952	T9612	T9612	ROOT	was,was
R7953	T9613	T9612	acomp	siRNA-transfected,was
R7954	T9614	T9613	cc	and,siRNA-transfected
R7955	T9615	T9613	conj	HIV-1-infected,siRNA-transfected
R7956	T9616	T9612	prep	at,was
R7957	T9617	T9618	amod	12-h,post-infection
R7958	T9618	T9616	pobj	post-infection,at
R7959	T9619	T9622	punct	(,S1
R7960	T9620	T9622	compound	Supplementary,S1
R7961	T9621	T9622	compound	Figure,S1
R7962	T9622	T9618	appos	S1,post-infection
R7963	T9623	T9622	punct	),S1
R7964	T9624	T9612	punct	.,was
R7965	T9625	T9626	det	The,expression
R7966	T9626	T9630	nsubjpass	expression,detected
R7967	T9627	T9626	prep	of,expression
R7968	T9628	T9627	pobj	Tat,of
R7969	T9629	T9630	auxpass	was,detected
R7970	T9630	T9630	ROOT	detected,detected
R7971	T9631	T9630	prep	at,detected
R7972	T9632	T9633	compound	1-h,post-infection
R7973	T9633	T9631	pobj	post-infection,at
R7974	T9634	T9630	cc	and,detected
R7975	T9635	T9636	advmod	progressively,increased
R7976	T9636	T9630	conj	increased,detected
R7977	T9637	T9636	prt	up,increased
R7978	T9638	T9636	prep	to,increased
R7979	T9639	T9640	nummod	12,h
R7980	T9640	T9638	pobj	h,to
R7981	T9641	T9640	prep	in,h
R7982	T9642	T9646	amod	untransfected,cells
R7983	T9643	T9642	cc	and,untransfected
R7984	T9644	T9642	conj	siRNA,untransfected
R7985	T9645	T9646	amod	control-transfected,cells
R7986	T9646	T9641	pobj	cells,in
R7987	T9647	T9636	punct	",",increased
R7988	T9648	T9652	mark	while,detected
R7989	T9649	T9652	nsubjpass	it,detected
R7990	T9650	T9652	auxpass	was,detected
R7991	T9651	T9652	advmod	barely,detected
R7992	T9652	T9636	advcl	detected,increased
R7993	T9653	T9652	prep	in,detected
R7994	T9654	T9653	pobj	siRNA,in
R7995	T9655	T9656	amod	Tat-transfected,cells
R7996	T9656	T9652	dobj	cells,detected
R7997	T9657	T9658	punct	(,Figure
R7998	T9658	T9656	parataxis	Figure,cells
R7999	T9659	T9658	nummod	1A,Figure
R8000	T9660	T9658	punct	),Figure
R8001	T9661	T9630	punct	.,detected
R8002	T9662	T9665	det	The,expression
R8003	T9663	T9665	amod	LTR-dependent,expression
R8004	T9664	T9665	compound	luciferase,expression
R8005	T9665	T9669	nsubjpass	expression,induced
R8006	T9666	T9669	auxpass	was,induced
R8007	T9667	T9666	advmod	also,was
R8008	T9668	T9669	advmod	progressively,induced
R8009	T9669	T9669	ROOT	induced,induced
R8010	T9670	T9669	prep	at,induced
R8011	T9671	T9670	pobj	1,at
R8012	T9672	T9669	punct	-,induced
R8013	T9673	T9669	prep	to,induced
R8014	T9674	T9675	amod	12-h,post-infection
R8015	T9675	T9673	pobj	post-infection,to
R8016	T9676	T9675	prep	in,post-infection
R8017	T9677	T9681	amod	untransfected,cells
R8018	T9678	T9677	cc	and,untransfected
R8019	T9679	T9677	conj	siRNA,untransfected
R8020	T9680	T9681	amod	control-transfected,cells
R8021	T9681	T9676	pobj	cells,in
R8022	T9682	T9669	punct	",",induced
R8023	T9683	T9687	mark	while,observed
R8024	T9684	T9687	nsubjpass	it,observed
R8025	T9685	T9687	auxpass	was,observed
R8026	T9686	T9687	advmod	barely,observed
R8027	T9687	T9669	advcl	observed,induced
R8028	T9688	T9687	prep	in,observed
R8029	T9689	T9688	pobj	siRNA,in
R8030	T9690	T9691	amod	Tat-transfected,cells
R8031	T9691	T9687	dobj	cells,observed
R8032	T9692	T9687	punct	",",observed
R8033	T9693	T9694	mark	as,expected
R8034	T9694	T9687	advcl	expected,observed
R8035	T9695	T9694	prep	for,expected
R8036	T9696	T9698	det	the,transactivation
R8037	T9697	T9698	amod	Tat-dependent,transactivation
R8038	T9698	T9695	pobj	transactivation,for
R8039	T9699	T9698	prep	of,transactivation
R8040	T9700	T9702	det	the,LTR
R8041	T9701	T9702	compound	HIV-1,LTR
R8042	T9702	T9699	pobj	LTR,of
R8043	T9703	T9705	punct	(,1A
R8044	T9704	T9705	compound	Figure,1A
R8045	T9705	T9702	appos	1A,LTR
R8046	T9706	T9702	punct	),LTR
R8047	T9707	T9669	punct	.,induced
R8048	T9708	T9710	det	The,increase
R8049	T9709	T9710	amod	progressive,increase
R8050	T9710	T9741	nsubjpass	increase,observed
R8051	T9711	T9710	prep	in,increase
R8052	T9712	T9713	amod	NF-κB,activity
R8053	T9713	T9711	pobj	activity,in
R8054	T9714	T9710	punct	",",increase
R8055	T9715	T9716	mark	as,measured
R8056	T9716	T9710	advcl	measured,increase
R8057	T9717	T9716	agent	by,measured
R8058	T9718	T9717	pobj	p65,by
R8059	T9719	T9718	amod	p50,p65
R8060	T9720	T9718	amod	binding,p65
R8061	T9721	T9720	prep	to,binding
R8062	T9722	T9724	det	an,consensus
R8063	T9723	T9724	amod	NF-κB,consensus
R8064	T9724	T9721	pobj	consensus,to
R8065	T9725	T9724	appos	oligonucleotide,consensus
R8066	T9726	T9727	punct	(,Figure
R8067	T9727	T9728	nmod	Figure,1B
R8068	T9728	T9725	appos	1B,oligonucleotide
R8069	T9729	T9728	punct	),1B
R8070	T9730	T9728	cc	and,1B
R8071	T9731	T9732	amod	nuclear,p65
R8072	T9732	T9728	conj	p65,1B
R8073	T9733	T9734	punct	(,Figure
R8074	T9734	T9724	appos	Figure,consensus
R8075	T9735	T9734	nummod	1C,Figure
R8076	T9736	T9734	punct	",",Figure
R8077	T9737	T9734	appos	nucleus,Figure
R8078	T9738	T9734	punct	),Figure
R8079	T9739	T9710	punct	",",increase
R8080	T9740	T9741	auxpass	was,observed
R8081	T9741	T9775	ccomp	observed,binding
R8082	T9742	T9741	prep	at,observed
R8083	T9743	T9745	meta	1,to
R8084	T9744	T9745	punct	-,to
R8085	T9745	T9741	prep	to,observed
R8086	T9746	T9747	amod	3-h,post-infection
R8087	T9747	T9745	pobj	post-infection,to
R8088	T9748	T9747	prep	in,post-infection
R8089	T9749	T9750	det	both,Tat-positive
R8090	T9750	T9748	pobj	Tat-positive,in
R8091	T9751	T9750	punct	(,Tat-positive
R8092	T9752	T9753	det	no,siRNA
R8093	T9753	T9750	appos	siRNA,Tat-positive
R8094	T9754	T9753	cc	or,siRNA
R8095	T9755	T9756	compound	siRNA,control
R8096	T9756	T9753	conj	control,siRNA
R8097	T9757	T9753	punct	),siRNA
R8098	T9758	T9750	cc	and,Tat-positive
R8099	T9759	T9750	conj	Tat-negative,Tat-positive
R8100	T9760	T9762	punct	(,Tat
R8101	T9761	T9762	compound	siRNA,Tat
R8102	T9762	T9750	appos	Tat,Tat-positive
R8103	T9763	T9762	punct	),Tat
R8104	T9764	T9748	pobj	cells,in
R8105	T9765	T9775	punct	;,binding
R8106	T9766	T9775	advmod	however,binding
R8107	T9767	T9775	punct	",",binding
R8108	T9768	T9775	prep	at,binding
R8109	T9769	T9770	amod	12-h,post-infection
R8110	T9770	T9768	pobj	post-infection,at
R8111	T9771	T9775	punct	",",binding
R8112	T9772	T9774	det	the,DNA
R8113	T9773	T9774	nummod	p65,DNA
R8114	T9774	T9775	nsubj	DNA,binding
R8115	T9775	T9778	amod	binding,p65
R8116	T9776	T9775	cc	and,binding
R8117	T9777	T9775	conj	nuclear,binding
R8118	T9778	T9779	nsubj	p65,persisted
R8119	T9779	T9779	ROOT	persisted,persisted
R8120	T9780	T9779	conj	elevated,persisted
R8121	T9781	T9780	prep	in,elevated
R8122	T9782	T9781	pobj	presence,in
R8123	T9783	T9782	prep	of,presence
R8124	T9784	T9783	pobj	Tat,of
R8125	T9785	T9782	punct	(,presence
R8126	T9786	T9787	det	no,siRNA
R8127	T9787	T9782	appos	siRNA,presence
R8128	T9788	T9787	cc	and,siRNA
R8129	T9789	T9790	compound	siRNA,control
R8130	T9790	T9787	conj	control,siRNA
R8131	T9791	T9787	punct	),siRNA
R8132	T9792	T9780	punct	",",elevated
R8133	T9793	T9795	mark	while,dropped
R8134	T9794	T9795	nsubj	they,dropped
R8135	T9795	T9780	advcl	dropped,elevated
R8136	T9796	T9795	prep	in,dropped
R8137	T9797	T9796	pobj	absence,in
R8138	T9798	T9797	prep	of,absence
R8139	T9799	T9798	pobj	Tat,of
R8140	T9800	T9799	punct	(,Tat
R8141	T9801	T9802	compound	siRNA,Tat
R8142	T9802	T9799	appos	Tat,Tat
R8143	T9803	T9799	punct	),Tat
R8144	T9804	T9805	punct	(,Figure
R8145	T9805	T9795	parataxis	Figure,dropped
R8146	T9806	T9805	nummod	1B,Figure
R8147	T9807	T9805	cc	and,Figure
R8148	T9808	T9805	conj	C,Figure
R8149	T9809	T9805	punct	),Figure
R8150	T9810	T9779	punct	.,persisted
R8151	T9811	T9813	nsubjpass	IκB-α,degraded
R8152	T9812	T9813	auxpass	was,degraded
R8153	T9813	T9813	ROOT	degraded,degraded
R8154	T9814	T9813	prep	in,degraded
R8155	T9815	T9816	det	the,cytosol
R8156	T9816	T9814	pobj	cytosol,in
R8157	T9817	T9816	prep	within,cytosol
R8158	T9818	T9819	compound	3-h,post-infection
R8159	T9819	T9817	pobj	post-infection,within
R8160	T9820	T9814	cc	and,in
R8161	T9821	T9823	mark	de,synthesized
R8162	T9822	T9823	nsubj	novo,synthesized
R8163	T9823	T9813	advcl	synthesized,degraded
R8164	T9824	T9823	prep	at,synthesized
R8165	T9825	T9826	nummod	6,h
R8166	T9826	T9824	pobj	h,at
R8167	T9827	T9823	prep	with,synthesized
R8168	T9828	T9829	amod	full,replenishment
R8169	T9829	T9827	pobj	replenishment,with
R8170	T9830	T9829	prep	at,replenishment
R8171	T9831	T9832	amod	12-h,post-infection
R8172	T9832	T9830	pobj	post-infection,at
R8173	T9833	T9823	prep	in,synthesized
R8174	T9834	T9835	preconj	both,Tat-positive
R8175	T9835	T9838	amod	Tat-positive,cells
R8176	T9836	T9835	cc	and,Tat-positive
R8177	T9837	T9835	conj	Tat-negative,Tat-positive
R8178	T9838	T9833	pobj	cells,in
R8179	T9839	T9840	punct	(,Figure
R8180	T9840	T9840	ROOT	Figure,Figure
R8181	T9841	T9840	nummod	1C,Figure
R8182	T9842	T9840	punct	",",Figure
R8183	T9843	T9840	dep	cytosol,Figure
R8184	T9844	T9840	punct	),Figure
R8185	T9845	T9813	punct	.,degraded
R8186	T9846	T9852	advmod	Consistently,induced
R8187	T9847	T9852	punct	",",induced
R8188	T9848	T9850	det	the,activity
R8189	T9849	T9850	compound	IKK,activity
R8190	T9850	T9852	nsubjpass	activity,induced
R8191	T9851	T9852	auxpass	was,induced
R8192	T9852	T9852	ROOT	induced,induced
R8193	T9853	T9852	prep	at,induced
R8194	T9854	T9855	amod	1-h,post-infection
R8195	T9855	T9853	pobj	post-infection,at
R8196	T9856	T9852	punct	",",induced
R8197	T9857	T9852	cc	and,induced
R8198	T9858	T9852	conj	decreased,induced
R8199	T9859	T9858	prep	at,decreased
R8200	T9860	T9861	compound	3-h,post-infection
R8201	T9861	T9859	pobj	post-infection,at
R8202	T9862	T9858	advmod	independently,decreased
R8203	T9863	T9858	prep	of,decreased
R8204	T9864	T9865	det	the,presence
R8205	T9865	T9863	pobj	presence,of
R8206	T9866	T9865	prep	of,presence
R8207	T9867	T9866	pobj	Tat,of
R8208	T9868	T9870	punct	(,1D
R8209	T9869	T9870	compound	Figure,1D
R8210	T9870	T9867	appos	1D,Tat
R8211	T9871	T9870	punct	),1D
R8212	T9872	T9852	punct	.,induced
R8213	T9873	T9874	amod	Similar,kinetics
R8214	T9874	T9885	nsubjpass	kinetics,observed
R8215	T9875	T9874	prep	of,kinetics
R8216	T9876	T9877	amod	NF-κB,activity
R8217	T9877	T9875	pobj	activity,of
R8218	T9878	T9874	punct	",",kinetics
R8219	T9879	T9880	compound	IKK,activation
R8220	T9880	T9874	conj	activation,kinetics
R8221	T9881	T9880	cc	and,activation
R8222	T9882	T9883	amod	IκB-α,degradation/re-synthesis
R8223	T9883	T9880	conj	degradation/re-synthesis,activation
R8224	T9884	T9885	auxpass	were,observed
R8225	T9885	T9885	ROOT	observed,observed
R8226	T9886	T9885	prep	in,observed
R8227	T9887	T9889	amod	single-round,infection
R8228	T9888	T9889	amod	HIV-1,infection
R8229	T9889	T9886	pobj	infection,in
R8230	T9890	T9889	prep	of,infection
R8251	T9911	T9912	compound	Figure,S2
R8252	T9912	T9908	appos	S2,inhibitors
R8253	T9913	T9908	punct	),inhibitors
R8254	T9914	T9885	punct	.,observed
R8255	T9915	T9916	det	These,results
R8256	T9916	T9917	nsubj	results,indicated
R8257	T9917	T9917	ROOT	indicated,indicated
R8258	T9918	T9927	mark	that,correlated
R8259	T9919	T9927	prep	in,correlated
R8260	T9920	T9922	amod	single-round,infection
R8261	T9921	T9922	compound	HIV-1,infection
R8262	T9922	T9919	pobj	infection,in
R8263	T9923	T9925	det	the,activation
R8264	T9924	T9925	compound	NF-κB,activation
R8265	T9925	T9927	nsubj	activation,correlated
R8266	T9926	T9927	advmod	initially,correlated
R8267	T9927	T9917	ccomp	correlated,indicated
R8268	T9928	T9927	prep	with,correlated
R8269	T9929	T9931	det	the,activation
R8270	T9930	T9931	compound	IKK,activation
R8271	T9931	T9928	pobj	activation,with
R8272	T9932	T9931	cc	and,activation
R8273	T9933	T9934	amod	IκB-α,degradation
R8274	T9934	T9931	conj	degradation,activation
R8275	T9935	T9934	advmod	independently,degradation
R8276	T9936	T9931	prep	of,activation
R8277	T9937	T9938	det	the,presence
R8278	T9938	T9936	pobj	presence,of
R8279	T9939	T9938	prep	of,presence
R8280	T9940	T9939	pobj	Tat,of
R8281	T9941	T9927	punct	",",correlated
R8282	T9942	T9927	cc	and,correlated
R8283	T9943	T9945	nsubjpass	it,kept
R8284	T9944	T9945	auxpass	was,kept
R8285	T9945	T9927	conj	kept,correlated
R8286	T9946	T9945	xcomp	elevated,kept
R8287	T9947	T9946	prep	in,elevated
R8288	T9948	T9947	pobj	presence,in
R8289	T9949	T9948	prep	of,presence
R8290	T9950	T9949	pobj	Tat,of
R8291	T9951	T9948	prep	following,presence
R8292	T9952	T9953	det	the,decay
R8293	T9953	T9951	pobj	decay,following
R8294	T9954	T9953	prep	of,decay
R8295	T9955	T9957	det	the,activity
R8296	T9956	T9957	compound	IKK,activity
R8297	T9957	T9954	pobj	activity,of
R8298	T9958	T9957	cc	and,activity
R8299	T9959	T9960	amod	new,synthesis
R8300	T9960	T9957	conj	synthesis,activity
R8301	T9961	T9960	prep	of,synthesis
R8302	T9962	T9961	pobj	IκB-α,of
R8303	T9963	T9917	punct	.,indicated
R8304	T9964	T9967	advmod	Next,tested
R8305	T9965	T9967	punct	",",tested
R8306	T9966	T9967	nsubj	we,tested
R8307	T9967	T9967	ROOT	tested,tested
R8308	T9968	T9970	det	the,action
R8309	T9969	T9970	amod	single,action
R8310	T9970	T9967	dobj	action,tested
R8311	T9971	T9970	prep	of,action
R8312	T9972	T9971	pobj	Tat,of
R8313	T9973	T9970	prep	on,action
R8314	T9974	T9975	det	the,kinetic
R8315	T9975	T9973	pobj	kinetic,on
R8316	T9976	T9975	prep	of,kinetic
R8317	T9977	T9978	amod	NF-κB,activity
R8318	T9978	T9976	pobj	activity,of
R8319	T9979	T9978	acl	induced,activity
R8320	T9980	T9979	agent	by,induced
R8321	T9981	T9980	pobj	PMA,by
R8322	T9982	T9967	punct	.,tested
R8323	T9983	T9990	prep	To,transfected
R8324	T9984	T9985	det	this,end
R8325	T9985	T9983	pobj	end,To
R8326	T9986	T9990	punct	",",transfected
R8327	T9987	T9988	compound	HeLa,cells
R8328	T9988	T9990	nsubjpass	cells,transfected
R8329	T9989	T9990	auxpass	were,transfected
R8330	T9990	T9990	ROOT	transfected,transfected
R8331	T9991	T9990	prep	with,transfected
R8332	T9992	T9991	pobj	p3x-FLAG-Tat,with
R8333	T9993	T9992	punct	",",p3x-FLAG-Tat
R8334	T9994	T9992	cc	or,p3x-FLAG-Tat
R8335	T9995	T9996	amod	empty,vector
R8336	T9996	T9992	conj	vector,p3x-FLAG-Tat
R8337	T9997	T9990	punct	",",transfected
R8338	T9998	T9990	dep	stimulated,transfected
R8339	T9999	T9998	prep	with,stimulated
R8340	T10000	T9999	pobj	PMA,with
R8341	T10001	T9998	prep	for,stimulated
R8342	T10002	T10003	nummod	5,min
R8343	T10003	T10001	pobj	min,for
R8344	T10004	T9998	punct	",",stimulated
R8345	T10005	T9990	cc	and,transfected
R8346	T10006	T10007	advmod	extensively,washed
R8347	T10007	T9990	conj	washed,transfected
R8348	T10008	T10009	aux	to,avoid
R8349	T10009	T10007	xcomp	avoid,washed
R8350	T10010	T10012	det	the,kinetic
R8351	T10011	T10012	amod	oscillatory,kinetic
R8352	T10012	T10009	dobj	kinetic,avoid
R8353	T10013	T10012	prep	of,kinetic
R8354	T10014	T10015	amod	NF-κB,activation
R8355	T10015	T10013	pobj	activation,of
R8356	T10016	T10015	punct	(,activation
R8357	T10017	T10015	appos	"17,18",activation
R8358	T10018	T10015	punct	),activation
R8359	T10019	T9990	punct	.,transfected
R8360	T10020	T10043	prep	In,enhanced
R8361	T10021	T10022	amod	un-stimulated,cells
R8362	T10022	T10020	pobj	cells,In
R8363	T10023	T10043	punct	",",enhanced
R8364	T10024	T10026	det	the,DNA
R8365	T10025	T10026	nummod	p65,DNA
R8366	T10026	T10043	nsubjpass	DNA,enhanced
R8367	T10027	T10043	amod	binding,enhanced
R8368	T10028	T10030	punct	(,2A
R8369	T10029	T10030	nmod	Figure,2A
R8370	T10030	T10027	appos	2A,binding
R8371	T10031	T10030	punct	),2A
R8372	T10032	T10030	cc	and,2A
R8373	T10033	T10034	amod	nuclear,p65
R8374	T10034	T10030	conj	p65,2A
R8375	T10035	T10037	punct	(,2B
R8376	T10036	T10037	compound	Figure,2B
R8377	T10037	T10043	nsubjpass	2B,enhanced
R8378	T10038	T10037	punct	",",2B
R8379	T10039	T10037	appos	nucleus,2B
R8380	T10040	T10037	punct	),2B
R8381	T10041	T10043	auxpass	were,enhanced
R8382	T10042	T10043	advmod	slightly,enhanced
R8383	T10043	T10043	ROOT	enhanced,enhanced
R8384	T10044	T10043	agent	by,enhanced
R8385	T10045	T10044	pobj	Tat,by
R8386	T10046	T10043	punct	",",enhanced
R8387	T10047	T10051	mark	while,was
R8388	T10048	T10050	det	the,content
R8389	T10049	T10050	amod	IκB-α,content
R8390	T10050	T10051	nsubj	content,was
R8391	T10051	T10043	advcl	was,enhanced
R8392	T10052	T10051	neg	not,was
R8393	T10053	T10054	advmod	significantly,affected
R8394	T10054	T10051	acomp	affected,was
R8395	T10055	T10057	punct	(,2B
R8396	T10056	T10057	compound	Figure,2B
R8397	T10057	T10051	advmod	2B,was
R8398	T10058	T10057	punct	",",2B
R8399	T10059	T10057	appos	cytosol,2B
R8400	T10060	T10057	punct	),2B
R8401	T10061	T10043	punct	.,enhanced
R8402	T10062	T10063	amod	Transient,stimulation
R8403	T10063	T10066	nsubj	stimulation,increased
R8404	T10064	T10063	prep	with,stimulation
R8405	T10065	T10064	pobj	PMA,with
R8406	T10066	T10106	ccomp	increased,persisted
R8407	T10067	T10071	det	the,activity
R8408	T10068	T10069	compound	NF-κB,DNA
R8409	T10069	T10071	nmod	DNA,activity
R8410	T10070	T10071	amod	binding,activity
R8411	T10071	T10066	dobj	activity,increased
R8412	T10072	T10071	cc	and,activity
R8413	T10073	T10074	amod	nuclear,p65
R8414	T10074	T10071	conj	p65,activity
R8415	T10075	T10066	prep	at,increased
R8416	T10076	T10077	nummod	5,min
R8417	T10077	T10075	pobj	min,at
R8418	T10078	T10066	advcl	peaking,increased
R8419	T10079	T10078	prep	at,peaking
R8420	T10080	T10079	pobj	60,at
R8421	T10081	T10082	nummod	120,min
R8422	T10082	T10066	npadvmod	min,increased
R8423	T10083	T10082	advmod	independently,min
R8424	T10084	T10083	prep	of,independently
R8425	T10085	T10086	det	the,presence
R8426	T10086	T10084	pobj	presence,of
R8427	T10087	T10086	prep	of,presence
R8428	T10088	T10087	pobj	Tat,of
R8429	T10089	T10091	punct	(,2A
R8430	T10090	T10091	compound	Figure,2A
R8431	T10091	T10086	appos	2A,presence
R8432	T10092	T10091	cc	and,2A
R8433	T10093	T10091	conj	B,2A
R8434	T10094	T10082	punct	),min
R8435	T10095	T10106	punct	;,persisted
R8436	T10096	T10106	advmod	however,persisted
R8437	T10097	T10106	punct	",",persisted
R8438	T10098	T10102	mark	while,binding
R8439	T10099	T10101	det	the,DNA
R8440	T10100	T10101	nummod	p65,DNA
R8441	T10101	T10102	nsubj	DNA,binding
R8442	T10102	T10105	amod	binding,p65
R8443	T10103	T10102	cc	and,binding
R8444	T10104	T10102	conj	nuclear,binding
R8445	T10105	T10106	nsubj	p65,persisted
R8446	T10106	T10106	ROOT	persisted,persisted
R8447	T10107	T10116	advcl	elevated,dropped
R8448	T10108	T10107	prep	at,elevated
R8449	T10109	T10110	amod	240-min,post-treatment
R8450	T10110	T10108	pobj	post-treatment,at
R8451	T10111	T10110	prep	in,post-treatment
R8452	T10112	T10113	amod	Tat-positive,cells
R8453	T10113	T10111	pobj	cells,in
R8454	T10114	T10116	punct	",",dropped
R8455	T10115	T10116	nsubj	they,dropped
R8456	T10116	T10106	conj	dropped,persisted
R8457	T10117	T10116	prep	in,dropped
R8458	T10118	T10119	amod	Tat-negative,cells
R8459	T10119	T10117	pobj	cells,in
R8460	T10120	T10121	punct	(,Figure
R8461	T10121	T10116	npadvmod	Figure,dropped
R8462	T10122	T10121	nummod	2A,Figure
R8463	T10123	T10121	cc	and,Figure
R8464	T10124	T10121	conj	B,Figure
R8465	T10125	T10121	punct	),Figure
R8466	T10126	T10116	punct	.,dropped
R8467	T10127	T10131	prep	In,bound
R8468	T10128	T10127	pobj	addition,In
R8469	T10129	T10131	punct	",",bound
R8470	T10130	T10131	nsubj	Tat,bound
R8480	T10140	T10142	poss	its,increased
R8481	T10141	T10142	advmod	binding,increased
R8482	T10142	T10131	dep	increased,bound
R8483	T10143	T10142	prep	following,increased
R8484	T10144	T10145	compound	PMA,stimulation
R8485	T10145	T10143	pobj	stimulation,following
R8486	T10146	T10148	punct	(,2A
R8487	T10147	T10148	compound	Figure,2A
R8488	T10148	T10145	appos	2A,stimulation
R8489	T10149	T10142	punct	),increased
R8490	T10150	T10131	punct	",",bound
R8491	T10151	T10131	advcl	suggesting,bound
R8492	T10152	T10156	mark	that,was
R8493	T10153	T10155	det	the,protein
R8494	T10154	T10155	amod	viral,protein
R8495	T10155	T10156	nsubj	protein,was
R8496	T10156	T10151	ccomp	was,suggesting
R8497	T10157	T10158	det	a,component
R8498	T10158	T10156	attr	component,was
R8499	T10159	T10158	prep	of,component
R8500	T10160	T10163	det	the,bound
R8501	T10161	T10163	amod	NF-κB,bound
R8502	T10162	T10163	amod	complex,bound
R8503	T10163	T10156	conj	bound,was
R8504	T10164	T10163	prep	to,bound
R8505	T10165	T10164	pobj	DNA,to
R8506	T10166	T10131	punct	.,bound
R8507	T10167	T10172	nsubjpass	Degradation,induced
R8508	T10168	T10167	prep	of,Degradation
R8509	T10169	T10168	pobj	IκB-α,of
R8510	T10170	T10172	auxpass	was,induced
R8511	T10171	T10172	advmod	progressively,induced
R8512	T10172	T10172	ROOT	induced,induced
R8513	T10173	T10172	prep	at,induced
R8514	T10174	T10173	pobj	5,at
R8515	T10175	T10176	nummod	30,min
R8516	T10176	T10176	ROOT	min,min
R8517	T10177	T10176	punct	",",min
R8518	T10178	T10176	acl	followed,min
R8519	T10179	T10178	agent	by,followed
R8520	T10180	T10182	nmod	de,synthesis
R8521	T10181	T10182	amod	novo,synthesis
R8522	T10182	T10179	pobj	synthesis,by
R8523	T10183	T10182	prep	at,synthesis
R8524	T10184	T10185	nummod	60,min
R8525	T10185	T10183	pobj	min,at
R8526	T10186	T10178	prep	with,followed
R8527	T10187	T10188	amod	full,replenishment
R8528	T10188	T10186	pobj	replenishment,with
R8529	T10189	T10178	prep	at,followed
R8530	T10190	T10191	nummod	240,min
R8531	T10191	T10189	pobj	min,at
R8532	T10192	T10191	prep	in,min
R8533	T10193	T10194	preconj	both,Tat-positive
R8534	T10194	T10197	amod	Tat-positive,cells
R8535	T10195	T10194	cc	and,Tat-positive
R8536	T10196	T10194	conj	negative,Tat-positive
R8537	T10197	T10192	pobj	cells,in
R8538	T10198	T10200	punct	(,2B
R8539	T10199	T10200	compound	Figure,2B
R8540	T10200	T10191	appos	2B,min
R8541	T10201	T10200	punct	",",2B
R8542	T10202	T10200	appos	cytosol,2B
R8543	T10203	T10200	punct	),2B
R8545	T10284	T10279	pobj	complex,of
R8547	T10285	T10273	punct	.,tested
R8549	T10286	T10297	prep	To,analysed
R8550	T10287	T10288	det	this,end
R8552	T10288	T10286	pobj	end,To
R8553	T10289	T10297	punct	",",analysed
R8554	T10290	T10291	det	the,association
R8556	T10291	T10297	nsubjpass	association,analysed
R8557	T10292	T10291	prep	of,association
R8558	T10293	T10292	pobj	IκB-α,of
R8560	T10294	T10291	prep	with,association
R8561	T10295	T10294	pobj	p65,with
R8562	T10296	T10297	auxpass	was,analysed
R8564	T10297	T10297	ROOT	analysed,analysed
R8565	T10298	T10297	prep	in,analysed
R8566	T10299	T10298	pobj	vivo,in
R8568	T10300	T10297	prep	at,analysed
R8569	T10301	T10304	nummod	0,min
R8571	T10302	T10301	cc	and,0
R8572	T10303	T10301	conj	240,0
R8574	T10304	T10300	pobj	min,at
R8576	T10305	T10304	prep	after,min
R8577	T10306	T10307	amod	short-pulse,PMA
R8579	T10307	T10305	pobj	PMA,after
R8580	T10308	T10307	punct	",",PMA
R8581	T10309	T10310	nsubj	which,corresponded
R8582	T10310	T10307	relcl	corresponded,PMA
R8583	T10311	T10310	prep	to,corresponded
R8584	T10312	T10313	det	the,time
R8586	T10313	T10311	pobj	time,to
R8587	T10314	T10313	prep	of,time
R8588	T10315	T10316	amod	un-stimulated,condition
R8590	T10316	T10314	pobj	condition,of
R8591	T10317	T10316	cc	and,condition
R8592	T10318	T10316	conj	post-activation,condition
R8594	T10319	T10321	nmod	de,synthesis
R8595	T10320	T10321	amod	novo,synthesis
R8597	T10321	T10316	conj	synthesis,condition
R8599	T10322	T10321	prep	of,synthesis
R8600	T10323	T10322	pobj	IκB-α,of
R8602	T10324	T10307	punct	",",PMA
R8603	T10325	T10297	advmod	respectively,analysed
R8605	T10326	T10297	punct	.,analysed
R8606	T10327	T10333	nsubjpass	Coimmunoprecipitation,detected
R8607	T10328	T10327	prep	of,Coimmunoprecipitation
R8609	T10329	T10328	pobj	IκB-α,of
R8610	T10330	T10327	prep	with,Coimmunoprecipitation
R8611	T10331	T10330	pobj	p65,with
R8613	T10332	T10333	auxpass	was,detected
R8614	T10333	T10333	ROOT	detected,detected
R8615	T10334	T10333	prep	at,detected
R8616	T10335	T10334	pobj	0,at
R8617	T10225	T10224	prep	of,presence
R8618	T10336	T10333	punct	-,detected
R8619	T10337	T10333	cc	and,detected
R8620	T10338	T10339	amod	240-min,post-treatment
R8621	T10226	T10225	pobj	Tat,of
R8622	T10339	T10347	nsubj	post-treatment,lanes
R8623	T10340	T10339	prep	in,post-treatment
R8624	T10227	T10228	punct	(,Figure
R8625	T10341	T10342	amod	Tat-negative,cells
R8626	T10342	T10340	pobj	cells,in
R8627	T10343	T10344	punct	(,Figure
R8628	T10228	T10226	appos	Figure,Tat
R8629	T10344	T10339	appos	Figure,post-treatment
R8630	T10345	T10344	nummod	2D,Figure
R8631	T10346	T10344	punct	",",Figure
R8632	T10347	T10347	ROOT	lanes,lanes
R8633	T10348	T10347	nummod	1,lanes
R8634	T10349	T10347	cc	and,lanes
R8635	T10350	T10347	conj	3,lanes
R8636	T10229	T10224	npadvmod	2C,presence
R8637	T10351	T10350	punct	),3
R8638	T10352	T10347	punct	",",lanes
R8639	T10353	T10333	cc	and,detected
R8640	T10230	T10221	punct	),independently
R8641	T10354	T10355	auxpass	was,halved
R8642	T10355	T10333	conj	halved,detected
R8643	T10231	T10210	punct	.,started
R8644	T10356	T10355	prep	in,halved
R8645	T10357	T10358	amod	Tat-positive,cells
R8646	T10358	T10356	pobj	cells,in
R8647	T10232	T10233	det	These,results
R8648	T10359	T10355	punct	",",halved
R8649	T10360	T10362	advmod	where,coimmunoprecipitated
R8650	T10361	T10362	nsubj	Tat,coimmunoprecipitated
R8651	T10233	T10234	nsubj	results,indicated
R8652	T10362	T10355	advcl	coimmunoprecipitated,halved
R8653	T10363	T10362	prep	with,coimmunoprecipitated
R8654	T10364	T10369	nmod	IκB-α,lanes
R8655	T10365	T10367	punct	(,2D
R8656	T10234	T10234	ROOT	indicated,indicated
R8657	T10366	T10367	compound	Figure,2D
R8658	T10367	T10364	appos	2D,IκB-α
R8659	T10235	T10237	mark	that,enhanced
R8660	T10368	T10367	punct	",",2D
R8661	T10369	T10363	pobj	lanes,with
R8662	T10370	T10369	nummod	2,lanes
R8663	T10236	T10237	nsubj	Tat,enhanced
R8664	T10371	T10370	cc	and,2
R8665	T10372	T10370	conj	4,2
R8666	T10373	T10355	punct	),halved
R8667	T10237	T10234	ccomp	enhanced,indicated
R8668	T10374	T10333	punct	",",detected
R8669	T10375	T10333	advcl	indicating,detected
R8670	T10376	T10378	mark	that,competed
R8671	T10238	T10240	det	the,activity
R8672	T10377	T10378	nsubj	Tat,competed
R8673	T10378	T10375	ccomp	competed,indicating
R8674	T10379	T10381	det	the,binding
R8675	T10239	T10240	amod	NF-κB,activity
R8676	T10380	T10381	amod	IκB-α,binding
R8677	T10240	T10237	dobj	activity,enhanced
R8678	T10241	T10240	prep	in,activity
R8679	T10381	T10378	dobj	binding,competed
R8680	T10382	T10381	prep	to,binding
R8681	T10242	T10245	amod	un-stimulated,cells
R8682	T10383	T10382	pobj	p65,to
R8683	T10243	T10242	cc	and,un-stimulated
R8684	T10384	T10333	punct	.,detected
R8685	T10244	T10242	conj	PMA-stimulated,un-stimulated
R8686	T10245	T10241	pobj	cells,in
R8687	T10246	T10237	prep	without,enhanced
R8688	T10247	T10246	pcomp	affecting,without
R8689	T10248	T10250	det	the,activity
R8690	T10249	T10250	compound	IKK,activity
R8691	T10250	T10247	dobj	activity,affecting
R8692	T10251	T10250	cc	and,activity
R8693	T10252	T10253	amod	IκB-α,content
R8694	T10253	T10250	conj	content,activity
R8695	T10254	T10234	punct	.,indicated
R8696	T10255	T10258	mark	As,found
R8697	T10256	T10258	nsubj	we,found
R8698	T10257	T10258	advmod	previously,found
R8699	T10258	T10273	advcl	found,tested
R8700	T10259	T10261	mark	that,binds
R8701	T10260	T10261	nsubj	Tat,binds
R8702	T10261	T10258	ccomp	binds,found
R8703	T10262	T10261	prep	to,binds
R8704	T10263	T10265	det	the,ankyrin
R8705	T10264	T10265	amod	sixth,ankyrin
R8706	T10265	T10262	pobj	ankyrin,to
R8707	T10266	T10265	prep	of,ankyrin
R8708	T10267	T10266	pobj	IκB-α,of
R8709	T10268	T10267	punct	(,IκB-α
R8710	T10269	T10267	parataxis	"50,51",IκB-α
R8711	T10270	T10267	punct	),IκB-α
R8712	T10271	T10273	punct	",",tested
R8713	T10272	T10273	nsubj	we,tested
R8714	T10273	T10273	ROOT	tested,tested
R8715	T10274	T10276	mark	whether,counteracted
R8716	T10275	T10276	nsubj	Tat,counteracted
R8717	T10276	T10273	ccomp	counteracted,tested
R8718	T10277	T10278	det	the,generation
R8719	T10278	T10276	dobj	generation,counteracted
R8720	T10279	T10278	prep	of,generation
R8721	T10280	T10284	det	the,complex
R8722	T10281	T10283	compound	IκB-α,NF-κB
R8723	T10282	T10283	compound	/,NF-κB
R8724	T10283	T10284	compound	NF-κB,complex
R19067	T23945	T23946	nsubj	Tat,counteracts
R19068	T23946	T23946	ROOT	counteracts,counteracts
R19069	T23947	T23949	det	the,turn
R19070	T23948	T23949	amod	post-activation,turn
R19071	T23949	T23946	dobj	turn,counteracts
R19072	T23950	T23949	prt	off,turn
R19073	T23951	T23949	prep	of,turn
R19074	T23952	T23951	pobj	NF-κB,of
R19075	T23953	T23949	prep	in,turn
R19076	T23954	T23956	amod	single,HIV-1-infection
R19077	T23955	T23956	compound	round,HIV-1-infection
R19078	T23956	T23953	pobj	HIV-1-infection,in
R19079	T23957	T23946	punct	.,counteracts
R19080	T23958	T23959	compound	Jurkat,cells
R19081	T23959	T23966	nsubjpass	cells,transfected
R19082	T23960	T23959	punct	(,cells
R19083	T23961	T23963	quantmod	5,107
R19084	T23962	T23963	quantmod	×,107
R19085	T23963	T23959	appos	107,cells
R19086	T23964	T23959	punct	),cells
R19087	T23965	T23966	auxpass	were,transfected
R19088	T23966	T23988	ccomp	transfected,infected
R19089	T23967	T23966	prep	with,transfected
R19090	T23968	T23969	compound	siRNA,Tat
R19091	T23969	T23967	pobj	Tat,with
R19092	T23970	T23969	cc	or,Tat
R19093	T23971	T23972	compound	siRNA,control
R19094	T23972	T23969	conj	control,Tat
R19095	T23973	T23972	punct	(,control
R19096	T23974	T23975	nummod	2,nmol
R19097	T23975	T23972	appos	nmol,control
R19098	T23976	T23972	punct	),control
R19099	T23977	T23972	punct	",",control
R19100	T23978	T23966	cc	or,transfected
R19101	T23979	T23966	conj	left,transfected
R19102	T23980	T23979	oprd	untransfected,left
R19103	T23981	T23988	punct	;,infected
R19104	T23982	T23983	nummod	24,h
R19105	T23983	T23984	npadvmod	h,later
R19106	T23984	T23988	advmod	later,infected
R19107	T23985	T23988	punct	",",infected
R19108	T23986	T23988	nsubjpass	cells,infected
R19109	T23987	T23988	auxpass	were,infected
R19110	T23988	T23988	ROOT	infected,infected
R19111	T23989	T23988	prep	with,infected
R19112	T23990	T23991	amod	HXB2-pseudotyped,NL4-3
R19113	T23991	T23989	pobj	NL4-3,with
R19114	T23992	T23988	punct	.,infected
R19115	T23993	T23997	compound	Luc.R,virions
R19116	T23994	T23997	compound	−,virions
R19117	T23995	T23996	compound	E,−
R19118	T23996	T23997	compound	−,virions
R19119	T23997	T24005	nsubj	virions,harvested
R19120	T23998	T24000	punct	(,ng
R19121	T23999	T24000	nummod	500,ng
R19122	T24000	T23997	appos	ng,virions
R19123	T24001	T24000	prep	of,ng
R19124	T24002	T24001	pobj	p24,of
R19125	T24003	T24000	punct	),ng
R19126	T24004	T23997	cc	and,virions
R19127	T24005	T24005	ROOT	harvested,harvested
R19128	T24006	T24005	prep	at,harvested
R19129	T24007	T24009	det	the,time
R19130	T24008	T24009	amod	indicated,time
R19131	T24009	T24006	pobj	time,at
R19132	T24010	T24005	punct	.,harvested
R19133	T24011	T24011	ROOT	(,(
R19134	T24012	T24011	dep	A,(
R19135	T24013	T24012	punct	),A
R19136	T24014	T24015	compound	Tat,expression
R19137	T24015	T24017	nsubjpass	expression,measured
R19138	T24016	T24017	auxpass	was,measured
R19139	T24017	T24017	ROOT	measured,measured
R19140	T24018	T24017	prep	in,measured
R19141	T24019	T24020	amod	total,RNA
R19142	T24020	T24018	pobj	RNA,in
R19143	T24021	T24017	agent	by,measured
R19144	T24022	T24023	amod	real-time,PCR
R19145	T24023	T24021	pobj	PCR,by
R19146	T24024	T24017	punct	",",measured
R19147	T24025	T24030	mark	while,measured
R19148	T24026	T24028	det	the,activity
R19149	T24027	T24028	compound	luciferase,activity
R19150	T24028	T24030	nsubjpass	activity,measured
R19151	T24029	T24030	auxpass	was,measured
R19152	T24030	T24017	advcl	measured,measured
R19153	T24031	T24030	prep	in,measured
R19154	T24032	T24034	amod	whole,extracts
R19155	T24033	T24034	compound	cell,extracts
R19156	T24034	T24031	pobj	extracts,in
R19157	T24035	T24017	punct	.,measured
R19158	T24036	T24045	punct	(,were
R19159	T24037	T24040	compound	B,extracts
R19160	T24038	T24039	punct	),Nuclear
R19161	T24039	T24040	compound	Nuclear,extracts
R19162	T24040	T24045	nsubj	extracts,were
R19163	T24041	T24040	punct	(,extracts
R19164	T24042	T24043	nummod	10,µg
R19165	T24043	T24040	appos	µg,extracts
R19166	T24044	T24040	punct	),extracts
R19167	T24045	T24045	ROOT	were,were
R19168	T24046	T24045	attr	analysed,were
R19169	T24047	T24046	prep	for,analysed
R19170	T24048	T24049	det	the,p65
R19171	T24049	T24047	pobj	p65,for
R19172	T24050	T24049	cc	and,p65
R19173	T24051	T24052	amod	p50,binding
R19174	T24052	T24045	acomp	binding,were
R19175	T24053	T24052	prep	to,binding
R19176	T24054	T24057	det	the,oligonucleotide
R19177	T24055	T24057	nmod	NF-κB,oligonucleotide
R19178	T24056	T24057	amod	double-stranded,oligonucleotide
R19179	T24057	T24053	pobj	oligonucleotide,to
R19180	T24058	T24045	advcl	using,were
R19181	T24059	T24065	det	the,kit
R19182	T24060	T24065	compound	NF-κB,kit
R19183	T24061	T24065	compound	Transcription,kit
R19184	T24062	T24064	compound	Factor,Assay
R19185	T24063	T24064	compound	ELISA,Assay
R19186	T24064	T24065	compound	Assay,kit
R19187	T24065	T24058	dobj	kit,using
R19188	T24066	T24065	punct	(,kit
R19189	T24067	T24065	appos	Cayman,kit
R19190	T24068	T24065	punct	),kit
R19191	T24069	T24045	punct	.,were
R19192	T24070	T24071	punct	(,C
R19193	T24071	T24071	ROOT	C,C
R19194	T24072	T24071	punct	),C
R19195	T24073	T24074	det	The,expression
R19196	T24074	T24080	nsubjpass	expression,analysed
R19197	T24075	T24074	prep	of,expression
R19198	T24076	T24075	pobj	p65,of
R19199	T24077	T24076	cc	and,p65
R19200	T24078	T24075	pobj	IκB-α,of
R19201	T24079	T24080	auxpass	was,analysed
R19202	T24080	T24080	ROOT	analysed,analysed
R19203	T24081	T24080	agent	by,analysed
R19204	T24082	T24083	nummod	12,%
R19205	T24083	T24084	compound	%,SDS
R19206	T24084	T24081	pobj	SDS,by
R19207	T24085	T24084	conj	PAGE,SDS
R19208	T24086	T24085	cc	and,PAGE
R19209	T24087	T24088	amod	western,blotting
R19210	T24088	T24085	conj	blotting,PAGE
R19211	T24089	T24088	prep	of,blotting
R19212	T24090	T24093	amod	nuclear,extracts
R19213	T24091	T24090	cc	or,nuclear
R19214	T24092	T24090	conj	cytosolic,nuclear
R19215	T24093	T24089	pobj	extracts,of
R19216	T24094	T24093	punct	(,extracts
R19217	T24095	T24096	nummod	20,µg
R19218	T24096	T24093	appos	µg,extracts
R19219	T24097	T24096	punct	),µg
R19220	T24098	T24088	advcl	using,blotting
R19221	T24099	T24102	amod	anti-p65,antibodies
R19222	T24100	T24099	cc	and,anti-p65
R19223	T24101	T24099	conj	anti-IκB-α,anti-p65
R19224	T24102	T24098	dobj	antibodies,using
R19225	T24103	T24080	punct	.,analysed
R19226	T24104	T24105	compound	Histone,H1
R19227	T24105	T24110	nsubjpass	H1,detected
R19228	T24106	T24105	cc	and,H1
R19229	T24107	T24108	compound	Hexokinase,II
R19230	T24108	T24105	conj	II,H1
R19231	T24109	T24110	auxpass	were,detected
R19232	T24110	T24110	ROOT	detected,detected
R19233	T24111	T24110	prep	with,detected
R19234	T24112	T24113	amod	specific,antibodies
R19235	T24113	T24111	pobj	antibodies,with
R19236	T24114	T24113	prep	as,antibodies
R19237	T24115	T24114	pobj	markers,as
R19238	T24116	T24115	prep	of,markers
R19239	T24117	T24120	amod	nuclear,extracts
R19240	T24118	T24117	cc	and,nuclear
R19241	T24119	T24117	conj	cytosolic,nuclear
R19242	T24120	T24116	pobj	extracts,of
R19243	T24121	T24120	punct	",",extracts
R19244	T24122	T24110	advmod	respectively,detected
R19245	T24123	T24110	punct	.,detected
R19246	T24124	T24125	compound	Densitometry,values
R19247	T24125	T24133	nsubjpass	values,expressed
R19248	T24126	T24127	punct	(,D
R19249	T24127	T24125	appos	D,values
R19250	T24128	T24125	punct	),values
R19251	T24129	T24125	prep	of,values
R19252	T24130	T24131	det	the,bands
R19253	T24131	T24129	pobj	bands,of
R19254	T24132	T24133	auxpass	were,expressed
R19255	T24133	T24133	ROOT	expressed,expressed
R19256	T24134	T24133	prep	as,expressed
R19257	T24135	T24136	compound	fold,increase
R19258	T24136	T24134	pobj	increase,as
R19259	T24137	T24136	prep	above,increase
R19260	T24138	T24139	det	the,control
R19261	T24139	T24137	pobj	control,above
R19262	T24140	T24139	punct	(,control
R19263	T24141	T24139	amod	mock,control
R19264	T24142	T24139	punct	),control
R19265	T24143	T24133	punct	.,expressed
R19266	T24144	T24150	punct	(,measured
R19267	T24145	T24148	compound	D,activity
R19268	T24146	T24147	punct	),IKK
R19269	T24147	T24148	compound	IKK,activity
R19270	T24148	T24150	nsubjpass	activity,measured
R19271	T24149	T24150	auxpass	was,measured
R19272	T24150	T24150	ROOT	measured,measured
R19273	T24151	T24150	prep	in,measured
R19274	T24152	T24153	amod	cytosolic,extracts
R19275	T24153	T24151	pobj	extracts,in
R19276	T24154	T24153	punct	(,extracts
R19277	T24155	T24156	nummod	100,µg
R19278	T24156	T24153	appos	µg,extracts
R19279	T24157	T24153	punct	),extracts
R19280	T24158	T24150	prep	by,measured
R19281	T24159	T24158	pcomp	using,by
R19282	T24160	T24163	compound	HTScan,Assay
R19283	T24161	T24163	compound	IKK,Assay
R19284	T24162	T24163	compound	Kinase,Assay
R19285	T24163	T24159	dobj	Assay,using
R19286	T24164	T24167	punct	(,Technology
R19287	T24165	T24167	compound	Cell,Technology
R19288	T24166	T24167	compound	Signaling,Technology
R19289	T24167	T24163	appos	Technology,Assay
R19290	T24168	T24163	punct	),Assay
R19291	T24169	T24150	punct	.,measured
R19292	T24170	T24181	nsubjpass	Values,shown
R19293	T24171	T24170	punct	(,Values
R19294	T24172	T24174	amod	mean,SE
R19295	T24173	T24174	compound	±,SE
R19296	T24174	T24170	appos	SE,Values
R19297	T24175	T24174	punct	",",SE
R19298	T24176	T24181	nsubjpass	n,shown
R19299	T24177	T24178	punct	=,3
R19300	T24178	T24176	advmod	3,n
R19301	T24179	T24181	punct	),shown
R19302	T24180	T24181	auxpass	are,shown
R19303	T24181	T24181	ROOT	shown,shown
R19304	T24182	T24181	punct	.,shown
R19662	T24672	T24673	nsubj	Tat,enhances
R19663	T24673	T24673	ROOT	enhances,enhances
R19664	T24674	T24676	det	the,activity
R19665	T24675	T24676	amod	NF-κB,activity
R19666	T24676	T24673	dobj	activity,enhances
R19667	T24677	T24676	prep	following,activity
R19668	T24678	T24679	compound	PMA,stimulation
R19669	T24679	T24677	pobj	stimulation,following
R19670	T24680	T24673	prep	by,enhances
R19671	T24681	T24680	pcomp	associating,by
R19672	T24682	T24681	prep	with,associating
R19673	T24683	T24682	pobj	IκB-α,with
R19674	T24684	T24681	cc	and,associating
R19675	T24685	T24681	conj	counteracting,associating
R19676	T24686	T24688	det	the,repression
R19677	T24687	T24688	amod	NF-κB,repression
R19678	T24688	T24685	dobj	repression,counteracting
R19679	T24689	T24673	punct	.,enhances
R19680	T24690	T24691	compound	HeLa,cells
R19681	T24691	T24698	nsubjpass	cells,transfected
R19682	T24692	T24691	punct	(,cells
R19683	T24693	T24695	nummod	5,106
R19684	T24694	T24695	nummod	×,106
R19685	T24695	T24691	appos	106,cells
R19686	T24696	T24691	punct	),cells
R19687	T24697	T24698	auxpass	were,transfected
R19688	T24698	T24698	ROOT	transfected,transfected
R19689	T24699	T24698	prep	with,transfected
R19690	T24700	T24699	pobj	p3xFLAG-Tat,with
R19691	T24701	T24700	cc	or,p3xFLAG-Tat
R19692	T24702	T24700	conj	p3xFLAG,p3xFLAG-Tat
R19693	T24703	T24704	amod	empty,vector
R19694	T24704	T24702	appos	vector,p3xFLAG
R19695	T24705	T24704	punct	(,vector
R19696	T24706	T24707	nummod	5,µg
R19697	T24707	T24704	appos	µg,vector
R19698	T24708	T24704	punct	),vector
R19699	T24709	T24702	punct	",",p3xFLAG
R19700	T24710	T24702	cc	and,p3xFLAG
R19701	T24711	T24712	nummod	48,h
R19702	T24712	T24713	npadvmod	h,later
R19703	T24713	T24715	advmod	later,stimulated
R19704	T24714	T24715	auxpass	were,stimulated
R19705	T24715	T24698	advcl	stimulated,transfected
R19706	T24716	T24715	prep	with,stimulated
R19707	T24717	T24716	pobj	PMA,with
R19708	T24718	T24717	punct	(,PMA
R19709	T24719	T24720	nummod	5,min
R19710	T24720	T24717	appos	min,PMA
R19711	T24721	T24720	punct	",",min
R19712	T24722	T24723	nummod	20,ng/ml
R19713	T24723	T24720	appos	ng/ml,min
R19714	T24724	T24720	punct	),min
R19715	T24725	T24720	cc	or,min
R19716	T24726	T24698	conj	left,transfected
R19717	T24727	T24726	oprd	unstimulated,left
R19718	T24728	T24729	punct	",",washed
R19719	T24729	T24698	conj	washed,transfected
R19720	T24730	T24729	advmod	twice,washed
R19721	T24731	T24729	prep	with,washed
R19722	T24732	T24731	pobj	DMEM,with
R19723	T24733	T24729	cc	and,washed
R19724	T24734	T24729	conj	harvested,washed
R19725	T24735	T24734	prep	at,harvested
R19726	T24736	T24738	det	the,times
R19727	T24737	T24738	amod	indicated,times
R19728	T24738	T24735	pobj	times,at
R19729	T24739	T24729	punct	.,washed
R19730	T24740	T24743	amod	Nuclear,extracts
R19731	T24741	T24740	cc	and,Nuclear
R19732	T24742	T24740	conj	cytosolic,Nuclear
R19733	T24743	T24745	nsubjpass	extracts,prepared
R19734	T24744	T24745	auxpass	were,prepared
R19735	T24745	T24745	ROOT	prepared,prepared
R19736	T24746	T24745	prep	for,prepared
R19737	T24747	T24748	amod	further,analysis
R19738	T24748	T24746	pobj	analysis,for
R19739	T24749	T24745	punct	.,prepared
R19740	T24750	T24756	punct	(,analysed
R19741	T24751	T24754	nmod	A,extracts
R19742	T24752	T24751	punct	),A
R19743	T24753	T24754	compound	Nuclear,extracts
R19744	T24754	T24756	nsubjpass	extracts,analysed
R19745	T24755	T24756	auxpass	were,analysed
R19746	T24756	T24756	ROOT	analysed,analysed
R19747	T24757	T24756	prep	for,analysed
R19748	T24758	T24759	det	the,p65
R19749	T24759	T24757	pobj	p65,for
R19750	T24760	T24759	punct	",",p65
R19751	T24761	T24759	amod	p50,p65
R19752	T24762	T24761	cc	and,p50
R19753	T24763	T24761	conj	FLAG-Tat,p50
R19754	T24764	T24756	oprd	binding,analysed
R19755	T24765	T24764	prep	to,binding
R19756	T24766	T24769	det	the,oligonucleotide
R19757	T24767	T24769	nmod	NF-κB,oligonucleotide
R19758	T24768	T24769	amod	double-stranded,oligonucleotide
R19759	T24769	T24765	pobj	oligonucleotide,to
R19760	T24770	T24756	advcl	using,analysed
R19761	T24771	T24777	det	the,kit
R19762	T24772	T24775	compound	NF-κB,ELISA
R19763	T24773	T24775	compound	Transcription,ELISA
R19764	T24774	T24775	compound	Factor,ELISA
R19765	T24775	T24777	compound	ELISA,kit
R19766	T24776	T24777	compound	assay,kit
R19767	T24777	T24770	dobj	kit,using
R19768	T24778	T24777	punct	(,kit
R19769	T24779	T24777	appos	Cayman,kit
R19770	T24780	T24777	punct	),kit
R19771	T24781	T24756	punct	.,analysed
R19772	T24782	T24785	punct	(,Nuclear
R19773	T24783	T24785	nmod	B,Nuclear
R19774	T24784	T24785	punct	),Nuclear
R19775	T24785	T24788	nmod	Nuclear,extracts
R19776	T24786	T24785	cc	and,Nuclear
R19777	T24787	T24785	conj	cytosolic,Nuclear
R19778	T24788	T24794	nsubjpass	extracts,separated
R19779	T24789	T24788	punct	(,extracts
R19780	T24790	T24791	nummod	20,µg
R19781	T24791	T24788	appos	µg,extracts
R19782	T24792	T24788	punct	),extracts
R19783	T24793	T24794	auxpass	were,separated
R19784	T24794	T24794	ROOT	separated,separated
R19785	T24795	T24794	prep	by,separated
R19786	T24796	T24797	nummod	12,%
R19787	T24797	T24798	compound	%,SDS
R19788	T24798	T24795	pobj	SDS,by
R19789	T24799	T24798	conj	PAGE,SDS
R19790	T24800	T24794	cc	and,separated
R19791	T24801	T24794	conj	analysed,separated
R19792	T24802	T24801	agent	by,analysed
R19793	T24803	T24804	amod	western,blotting
R19794	T24804	T24802	pobj	blotting,by
R19795	T24805	T24801	advcl	using,analysed
R19796	T24806	T24812	det	the,H1
R19797	T24807	T24812	amod	anti-p65,H1
R19798	T24808	T24812	punct	",",H1
R19799	T24809	T24812	amod	anti-FLAG,H1
R19800	T24810	T24812	punct	",",H1
R19801	T24811	T24812	amod	anti-Histone,H1
R19802	T24812	T24805	dobj	H1,using
R19803	T24813	T24812	punct	",",H1
R19804	T24814	T24812	conj	anti-IκB-α,H1
R19805	T24815	T24814	cc	and,anti-IκB-α
R19806	T24816	T24814	conj	anti-Hexokinase-II,anti-IκB-α
R19807	T24817	T24812	conj	antibodies,H1
R19808	T24818	T24794	punct	.,separated
R19809	T24819	T24820	compound	Densitometry,values
R19810	T24820	T24828	nsubjpass	values,expressed
R19811	T24821	T24822	punct	(,D
R19812	T24822	T24820	appos	D,values
R19813	T24823	T24822	punct	),D
R19814	T24824	T24822	prep	of,D
R19815	T24825	T24826	det	the,bands
R19816	T24826	T24824	pobj	bands,of
R19817	T24827	T24828	auxpass	were,expressed
R19818	T24828	T24828	ROOT	expressed,expressed
R19819	T24829	T24830	mark	as,fold
R19820	T24830	T24828	advcl	fold,expressed
R19821	T24831	T24830	dobj	increase,fold
R19822	T24832	T24831	prep	above,increase
R19823	T24833	T24834	det	the,control
R19824	T24834	T24832	pobj	control,above
R19825	T24835	T24834	punct	(,control
R19826	T24836	T24834	appos	lane,control
R19827	T24837	T24836	nummod	1,lane
R19828	T24838	T24834	punct	),control
R19829	T24839	T24828	punct	.,expressed
R19830	T24840	T24846	punct	(,measured
R19831	T24841	T24844	nmod	C,activity
R19832	T24842	T24841	punct	),C
R19833	T24843	T24844	compound	IKK,activity
R19834	T24844	T24846	nsubjpass	activity,measured
R19835	T24845	T24846	auxpass	was,measured
R19836	T24846	T24846	ROOT	measured,measured
R19837	T24847	T24846	prep	in,measured
R19838	T24848	T24849	amod	cytosolic,extracts
R19839	T24849	T24847	pobj	extracts,in
R19840	T24850	T24849	punct	(,extracts
R19841	T24851	T24852	nummod	100,µg
R19842	T24852	T24849	appos	µg,extracts
R19843	T24853	T24849	punct	),extracts
R19844	T24854	T24846	prep	by,measured
R19845	T24855	T24854	pcomp	using,by
R19846	T24856	T24859	nmod	HTScan,assay
R19847	T24857	T24859	nmod	IKK,assay
R19848	T24858	T24859	compound	kinase,assay
R19849	T24859	T24855	dobj	assay,using
R19850	T24860	T24859	punct	(,assay
R19851	T24861	T24863	compound	Cell,Technology
R19852	T24862	T24863	compound	Signaling,Technology
R19853	T24863	T24859	appos	Technology,assay
R19854	T24864	T24859	punct	),assay
R19855	T24865	T24846	punct	.,measured
R19856	T24866	T24877	punct	(,transfected
R19857	T24867	T24870	compound	D,cells
R19858	T24868	T24869	punct	),HeLa
R19859	T24869	T24870	compound	HeLa,cells
R19860	T24870	T24877	nsubjpass	cells,transfected
R19861	T24871	T24870	punct	(,cells
R19862	T24872	T24874	nummod	5,106
R19863	T24873	T24874	nummod	×,106
R19864	T24874	T24870	appos	106,cells
R19865	T24875	T24870	punct	),cells
R19866	T24876	T24877	auxpass	were,transfected
R19867	T24877	T24893	ccomp	transfected,stimulated
R19868	T24878	T24877	prep	with,transfected
R19869	T24879	T24878	cc	or,with
R19870	T24880	T24878	conj	without,with
R19871	T24881	T24880	pobj	p3xFLAG-Tat,without
R19872	T24882	T24881	punct	(,p3xFLAG-Tat
R19873	T24883	T24884	nummod	5,µg
R19874	T24884	T24881	appos	µg,p3xFLAG-Tat
R19875	T24885	T24881	punct	),p3xFLAG-Tat
R19876	T24886	T24893	punct	;,stimulated
R19877	T24887	T24888	nummod	48,h
R19878	T24888	T24889	npadvmod	h,later
R19879	T24889	T24893	advmod	later,stimulated
R19880	T24890	T24893	punct	",",stimulated
R19881	T24891	T24893	nsubjpass	cells,stimulated
R19882	T24892	T24893	auxpass	were,stimulated
R19883	T24893	T24893	ROOT	stimulated,stimulated
R19884	T24894	T24893	prep	with,stimulated
R19885	T24895	T24894	pobj	PMA,with
R19886	T24896	T24895	punct	(,PMA
R19887	T24897	T24898	nummod	5,min
R19888	T24898	T24895	appos	min,PMA
R19889	T24899	T24898	punct	",",min
R19890	T24900	T24901	nummod	20,ng/ml
R19891	T24901	T24898	appos	ng/ml,min
R19892	T24902	T24898	punct	),min
R19893	T24903	T24898	cc	or,min
R19894	T24904	T24893	conj	left,stimulated
R19895	T24905	T24904	oprd	unstimulated,left
R19896	T24906	T24907	punct	",",washed
R19897	T24907	T24893	conj	washed,stimulated
R19898	T24908	T24907	advmod	twice,washed
R19899	T24909	T24907	prep	with,washed
R19900	T24910	T24909	pobj	DMEM,with
R19901	T24911	T24907	punct	",",washed
R19902	T24912	T24907	cc	and,washed
R19903	T24913	T24907	conj	harvested,washed
R19904	T24914	T24913	prep	after,harvested
R19905	T24915	T24916	nummod	240,min
R19906	T24916	T24914	pobj	min,after
R19907	T24917	T24893	punct	.,stimulated
R19908	T24918	T24920	amod	Whole,extracts
R19909	T24919	T24920	compound	cell,extracts
R19910	T24920	T24926	nsubjpass	extracts,immunoprecipitated
R19911	T24921	T24923	punct	(,mg
R19912	T24922	T24923	nummod	1,mg
R19913	T24923	T24920	appos	mg,extracts
R19914	T24924	T24920	punct	),extracts
R19915	T24925	T24926	auxpass	were,immunoprecipitated
R19916	T24926	T24926	ROOT	immunoprecipitated,immunoprecipitated
R19917	T24927	T24926	prep	with,immunoprecipitated
R19918	T24928	T24931	nmod	protein,antibody
R19919	T24929	T24931	amod	G-Sepharose-coupled,antibody
R19920	T24930	T24931	amod	anti-IκB-α,antibody
R19921	T24931	T24927	pobj	antibody,with
R19922	T24932	T24926	punct	.,immunoprecipitated
R19923	T24933	T24935	nsubjpass	Immunocomplexes,separated
R19924	T24934	T24935	auxpass	were,separated
R19925	T24935	T24935	ROOT	separated,separated
R19926	T24936	T24935	prep	by,separated
R19927	T24937	T24938	nummod	12,%
R19928	T24938	T24939	compound	%,SDS
R19929	T24939	T24936	pobj	SDS,by
R19930	T24940	T24939	conj	PAGE,SDS
R19931	T24941	T24935	cc	and,separated
R19932	T24942	T24935	conj	analysed,separated
R19933	T24943	T24942	agent	by,analysed
R19934	T24944	T24945	amod	western,blotting
R19935	T24945	T24943	pobj	blotting,by
R19936	T24946	T24945	prep	with,blotting
R19937	T24947	T24952	amod	anti-p65,antibodies
R19938	T24948	T24947	punct	",",anti-p65
R19939	T24949	T24947	conj	anti-FLAG,anti-p65
R19940	T24950	T24949	cc	and,anti-FLAG
R19941	T24951	T24949	conj	anti-IκB-α,anti-FLAG
R19942	T24952	T24946	pobj	antibodies,with
R19943	T24953	T24935	punct	.,separated
R19944	T24954	T24955	compound	Densitometry,values
R19945	T24955	T24963	nsubjpass	values,expressed
R19946	T24956	T24957	punct	(,D
R19947	T24957	T24955	appos	D,values
R19948	T24958	T24957	punct	),D
R19949	T24959	T24957	prep	of,D
R19950	T24960	T24961	det	the,bands
R19951	T24961	T24959	pobj	bands,of
R19952	T24962	T24963	auxpass	were,expressed
R19953	T24963	T24963	ROOT	expressed,expressed
R19954	T24964	T24963	prep	as,expressed
R19955	T24965	T24966	compound	fold,increase
R19956	T24966	T24964	pobj	increase,as
R19957	T24967	T24966	prep	above,increase
R19958	T24968	T24969	det	the,control
R19959	T24969	T24967	pobj	control,above
R19960	T24970	T24969	punct	(,control
R19961	T24971	T24969	appos	lane,control
R19962	T24972	T24971	nummod	1,lane
R19963	T24973	T24969	punct	),control
R19964	T24974	T24963	punct	.,expressed
R19965	T24975	T24986	nsubjpass	Values,shown
R19966	T24976	T24975	punct	(,Values
R19967	T24977	T24979	amod	mean,SE
R19968	T24978	T24979	compound	±,SE
R19969	T24979	T24975	appos	SE,Values
R19970	T24980	T24986	punct	",",shown
R19971	T24981	T24986	nsubjpass	n,shown
R19972	T24982	T24983	punct	=,3
R19973	T24983	T24981	nummod	3,n
R19974	T24984	T24986	punct	),shown
R19975	T24985	T24986	auxpass	are,shown
R19976	T24986	T24986	ROOT	shown,shown
R19977	T24987	T24986	punct	.,shown
R8231	T9891	T9890	pobj	PBMCs,of
R8232	T9892	T9885	advcl	using,observed
R8233	T9893	T9894	det	a,cocktail
R8234	T9894	T9892	dobj	cocktail,using
R8235	T9895	T9894	prep	of,cocktail
R8236	T9896	T9895	pobj	Azidothymidine,of
R8237	T9897	T9896	punct	(,Azidothymidine
R8238	T9898	T9896	appos	AZT,Azidothymidine
R8239	T9899	T9896	punct	),Azidothymidine
R8240	T9900	T9896	cc	and,Azidothymidine
R8241	T9901	T9896	conj	Lamivudine,Azidothymidine
R8242	T9902	T9901	punct	(,Lamivudine
R8243	T9903	T9901	appos	3TC,Lamivudine
R8244	T9904	T9901	punct	),Lamivudine
R8245	T9905	T9892	prep	as,using
R8246	T9906	T9908	compound	reverse,inhibitors
R8247	T9907	T9908	compound	transcriptase,inhibitors
R8248	T9908	T9905	pobj	inhibitors,as
R8249	T9909	T9912	punct	(,S2
R8250	T9910	T9912	compound	Supplementary,S2
R8471	T10131	T10131	ROOT	bound,bound
R8472	T10132	T10131	prep	to,bound
R8473	T10133	T10135	det	the,oligonucleotide
R8474	T10134	T10135	amod	NF-κB,oligonucleotide
R8475	T10135	T10132	pobj	oligonucleotide,to
R8476	T10136	T10135	prep	in,oligonucleotide
R8477	T10137	T10138	amod	un-stimulated,cells
R8478	T10138	T10136	pobj	cells,in
R8479	T10139	T10135	cc	and,oligonucleotide
R8544	T10204	T10178	punct	.,followed
R8546	T10205	T10210	advmod	Consistently,started
R8548	T10206	T10210	punct	",",started
R8551	T10207	T10209	det	the,activation
R8555	T10208	T10209	compound	IKK,activation
R8559	T10209	T10210	nsubj	activation,started
R8563	T10210	T10210	ROOT	started,started
R8567	T10211	T10210	prep	at,started
R8570	T10212	T10213	nummod	5,min
R8573	T10213	T10211	pobj	min,at
R8575	T10214	T10210	punct	",",started
R8578	T10215	T10210	cc	and,started
R8585	T10216	T10210	conj	turned,started
R8589	T10217	T10216	prt	off,turned
R8593	T10218	T10216	prep	at,turned
R8596	T10219	T10220	nummod	30,min
R8598	T10220	T10218	pobj	min,at
R8601	T10221	T10216	advmod	independently,turned
R8604	T10222	T10221	prep	of,independently
R8608	T10223	T10224	det	the,presence
R8612	T10224	T10222	pobj	presence,of

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T8411	3871-3882	http://purl.obolibrary.org/obo/UBERON_2000106	denotes	extensively

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T10407	131-147	http://purl.obolibrary.org/obo/GO_0051092	denotes	NF-κB activation
T10408	3435-3451	http://purl.obolibrary.org/obo/GO_0051092	denotes	NF-κB activation
T10409	3926-3942	http://purl.obolibrary.org/obo/GO_0051092	denotes	NF-κB activation
T10410	221-237	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNA interference
T10411	1015-1030	http://purl.obolibrary.org/obo/GO_2000144	denotes	transactivation
T10412	2758-2766	http://purl.obolibrary.org/obo/GO_0009056	denotes	degraded
T10413	3165-3176	http://purl.obolibrary.org/obo/GO_0009056	denotes	degradation
T10414	3507-3518	http://purl.obolibrary.org/obo/GO_0009056	denotes	degradation
T10415	2970-2973	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10416	3140-3143	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10417	3482-3485	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10418	3628-3631	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10419	4941-4944	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10420	5174-5177	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10421	3180-3189	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T10422	3649-3658	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T10423	4806-4815	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T10424	7171-7180	http://purl.obolibrary.org/obo/GO_0009058	denotes	synthesis
T10425	3318-3331	http://purl.obolibrary.org/obo/GO_0003899	denotes	transcriptase
T10426	3318-3331	http://purl.obolibrary.org/obo/GO_0003968	denotes	transcriptase
T10427	3318-3331	http://purl.obolibrary.org/obo/GO_0034062	denotes	transcriptase
T10428	3728-3750	http://purl.obolibrary.org/obo/GO_0004704	denotes	NF-κB activity induced
T10429	4798-4815	http://purl.obolibrary.org/obo/GO_0006189	denotes	de novo synthesis
T10430	7163-7180	http://purl.obolibrary.org/obo/GO_0006189	denotes	de novo synthesis
T10431	4798-4815	http://purl.obolibrary.org/obo/GO_0097294	denotes	de novo synthesis
T10432	7163-7180	http://purl.obolibrary.org/obo/GO_0097294	denotes	de novo synthesis
T24183	1466-1485	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T24184	1466-1485	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T24185	1466-1485	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T24186	1466-1485	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T24187	1466-1485	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T24188	1655-1668	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T24189	2098-2101	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T24190	2171-2174	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T24996	5769-5782	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T24997	6118-6121	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T24998	6191-6194	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T10460	2321-2328	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10461	2588-2595	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10462	3988-3995	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10463	4208-4215	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10464	4355-4362	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10465	4584-4591	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10466	7488-7495	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T10467	2321-2340	http://purl.obolibrary.org/obo/GO_0051059	denotes	binding to an NF-κB
T10468	2584-2595	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T10469	3984-3995	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T10470	4204-4215	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T10471	4351-4362	http://purl.obolibrary.org/obo/GO_0003677	denotes	DNA binding
T10472	2970-2973	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10473	3140-3143	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10474	3482-3485	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10475	3628-3631	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10476	4941-4944	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10477	5174-5177	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T10478	3318-3331	http://purl.obolibrary.org/obo/GO_0003899	denotes	transcriptase
T10479	3318-3331	http://purl.obolibrary.org/obo/GO_0003968	denotes	transcriptase
T10480	3318-3331	http://purl.obolibrary.org/obo/GO_0034062	denotes	transcriptase
T10481	3728-3750	http://purl.obolibrary.org/obo/GO_0004704	denotes	NF-κB activity induced
T24191	1466-1485	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T24192	1466-1485	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T24193	1466-1485	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T24194	1466-1485	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T24195	1466-1485	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T24196	1586-1593	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T24197	1586-1606	http://purl.obolibrary.org/obo/GO_0051059	denotes	binding to the NF-κB
T24198	1859-1869	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T24199	1928-1938	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T24200	2098-2101	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T24201	2171-2174	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T24999	5700-5707	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T25000	5700-5720	http://purl.obolibrary.org/obo/GO_0051059	denotes	binding to the NF-κB
T25001	6005-6015	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T25002	6676-6686	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T25003	6118-6121	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T25004	6191-6194	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T10433	246-251	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10434	338-343	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10435	446-451	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10436	686-691	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10437	747-752	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10438	913-918	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10439	974-979	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10440	2536-2541	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10441	2924-2929	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10442	3777-3782	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10443	3969-3974	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10444	4440-4445	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10445	4476-4481	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10446	4570-4575	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10447	4895-4900	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10448	5146-5151	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10449	7307-7312	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10450	7372-7377	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T10451	399-406	http://purl.obolibrary.org/obo/GO_0019012	denotes	virions
T10452	2407-2414	http://purl.obolibrary.org/obo/GO_0005634	denotes	nucleus
T10453	4036-4043	http://purl.obolibrary.org/obo/GO_0005634	denotes	nucleus
T10454	2774-2781	http://purl.obolibrary.org/obo/GO_0005829	denotes	cytosol
T10455	2942-2949	http://purl.obolibrary.org/obo/GO_0005829	denotes	cytosol
T10456	4143-4150	http://purl.obolibrary.org/obo/GO_0005829	denotes	cytosol
T10457	4913-4920	http://purl.obolibrary.org/obo/GO_0005829	denotes	cytosol
T10458	4698-4711	http://purl.obolibrary.org/obo/GO_0071159	denotes	NF-κB complex
T10459	6962-6975	http://purl.obolibrary.org/obo/GO_0071159	denotes	NF-κB complex
T24202	1165-1170	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T24203	1275-1280	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T24204	1508-1512	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T24205	1332-1339	http://purl.obolibrary.org/obo/GO_0019012	denotes	virions
T24206	1859-1869	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T24207	1928-1938	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T24208	1859-1869	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T24209	1928-1938	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T24988	5345-5350	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T24989	6246-6251	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T24990	6327-6332	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T24991	6457-6461	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T24992	6005-6015	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T24993	6676-6686	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T24994	6005-6015	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T24995	6676-6686	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies

sentences

Id	Subject	Object	Predicate	Lexical cue
T8532	0-103	Sentence	denotes	Tat enhances the NF-κB activity by hijacking IκB-α and inhibiting the post-activation turn off of NF-κB
T8533	104-238	Sentence	denotes	We analysed the kinetic of NF-κB activation in single round HIV-1 infection by modulating the expression of Tat with RNA interference.
T8534	239-407	Sentence	denotes	Jurkat cells were transfected with siRNA Tat, siRNA control, or left untransfected, and 24 h later cells were infected with HXB2 Env-pseudotyped NL4-3.Luc.R−E− virions.
T8535	408-542	Sentence	denotes	By cytofluorimetric analysis, ∼40% of cells was siRNA-transfected and HIV-1-infected at 12-h post-infection (Supplementary Figure S1).
T8536	543-765	Sentence	denotes	The expression of Tat was detected at 1-h post-infection and progressively increased up to 12 h in untransfected and siRNA control-transfected cells, while it was barely detected in siRNA Tat-transfected cells (Figure 1A).
T8537	766-1060	Sentence	denotes	The LTR-dependent luciferase expression was also progressively induced at 1- to 12-h post-infection in untransfected and siRNA control-transfected cells, while it was barely observed in siRNA Tat-transfected cells, as expected for the Tat-dependent transactivation of the HIV-1 LTR (Figure 1A).
T8538	2254-2747	Sentence	denotes	The progressive increase in NF-κB activity, as measured by p65–p50 binding to an NF-κB consensus oligonucleotide (Figure 1B) and nuclear p65 (Figure 1C, nucleus), was observed at 1- to 3-h post-infection in both Tat-positive (no siRNA or siRNA control) and Tat-negative (siRNA Tat) cells; however, at 12-h post-infection, the p65 DNA binding and nuclear p65 persisted elevated in presence of Tat (no siRNA and siRNA control), while they dropped in absence of Tat (siRNA Tat) (Figure 1B and C).
T8539	2748-2951	Sentence	denotes	IκB-α was degraded in the cytosol within 3-h post-infection and de novo synthesized at 6 h with full replenishment at 12-h post-infection in both Tat-positive and Tat-negative cells (Figure 1C, cytosol).
T8540	2952-3103	Sentence	denotes	Consistently, the IKK activity was induced at 1-h post-infection, and decreased at 3-h post-infection independently of the presence of Tat (Figure 1D).
T8541	3104-3369	Sentence	denotes	Similar kinetics of NF-κB activity, IKK activation and IκB-α degradation/re-synthesis were observed in single-round HIV-1 infection of PBMCs using a cocktail of Azidothymidine (AZT) and Lamivudine (3TC) as reverse transcriptase inhibitors (Supplementary Figure S2).
T8542	3370-3668	Sentence	denotes	These results indicated that in single-round HIV-1 infection the NF-κB activation initially correlated with the IKK activation and IκB-α degradation independently of the presence of Tat, and it was kept elevated in presence of Tat following the decay of the IKK activity and new synthesis of IκB-α.
T8543	3669-3758	Sentence	denotes	Next, we tested the single action of Tat on the kinetic of NF-κB activity induced by PMA.
T8544	3759-3951	Sentence	denotes	To this end, HeLa cells were transfected with p3x-FLAG-Tat, or empty vector, stimulated with PMA for 5 min, and extensively washed to avoid the oscillatory kinetic of NF-κB activation (17,18).
T8545	3952-4152	Sentence	denotes	In un-stimulated cells, the p65 DNA binding (Figure 2A) and nuclear p65 (Figure 2B, nucleus) were slightly enhanced by Tat, while the IκB-α content was not significantly affected (Figure 2B, cytosol).
T8546	4153-4500	Sentence	denotes	Transient stimulation with PMA increased the NF-κB DNA binding activity and nuclear p65 at 5 min peaking at 60–120 min independently of the presence of Tat (Figure 2A and B); however, while the p65 DNA binding and nuclear p65 persisted elevated at 240-min post-treatment in Tat-positive cells, they dropped in Tat-negative cells (Figure 2A and B).
T8547	4501-4725	Sentence	denotes	In addition, Tat bound to the NF-κB oligonucleotide in un-stimulated cells and its binding increased following PMA stimulation (Figure 2A), suggesting that the viral protein was a component of the NF-κB complex bound to DNA.
T8548	4726-4922	Sentence	denotes	Degradation of IκB-α was progressively induced at 5–30 min, followed by de novo synthesis at 60 min with full replenishment at 240 min in both Tat-positive and negative cells (Figure 2B, cytosol).
T8549	4923-5048	Sentence	denotes	Consistently, the IKK activation started at 5 min, and turned off at 30 min independently of the presence of Tat (Figure 2C).
T8550	5049-5205	Sentence	denotes	These results indicated that Tat enhanced the NF-κB activity in un-stimulated and PMA-stimulated cells without affecting the IKK activity and IκB-α content.
T8551	6822-6976	Sentence	denotes	As we previously found that Tat binds to the sixth ankyrin of IκB-α (50,51), we tested whether Tat counteracted the generation of the IκB-α/NF-κB complex.
T8552	6977-7204	Sentence	denotes	To this end, the association of IκB-α with p65 was analysed in vivo at 0 and 240 min after short-pulse PMA, which corresponded to the time of un-stimulated condition and post-activation de novo synthesis of IκB-α, respectively.
T8553	7205-7503	Sentence	denotes	Coimmunoprecipitation of IκB-α with p65 was detected at 0- and 240-min post-treatment in Tat-negative cells (Figure 2D, lanes 1 and 3), and was halved in Tat-positive cells, where Tat coimmunoprecipitated with IκB-α (Figure 2D, lanes 2 and 4), indicating that Tat competed the IκB-α binding to p65.
T23911	1071-1157	Sentence	denotes	Tat counteracts the post-activation turn off of NF-κB in single round HIV-1-infection.
T23912	1158-1870	Sentence	denotes	Jurkat cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.Luc.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Tat expression was measured in total RNA by real-time PCR, while the luciferase activity was measured in whole cell extracts. (B) Nuclear extracts (10 µg) were analysed for the p65 and p50 binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA Assay kit (Cayman). (C) The expression of p65 and IκB-α was analysed by 12% SDS–PAGE and western blotting of nuclear or cytosolic extracts (20 µg) using anti-p65 and anti-IκB-α antibodies.
T23913	1871-1998	Sentence	denotes	Histone H1 and Hexokinase II were detected with specific antibodies as markers of nuclear and cytosolic extracts, respectively.
T23914	1999-2216	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic extracts (100 µg) by using HTScan IKK Kinase Assay (Cell Signaling Technology).
T23915	2217-2253	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T24351	5216-5339	Sentence	denotes	Tat enhances the NF-κB activity following PMA stimulation by associating with IκB-α and counteracting the NF-κB repression.
T24352	5340-5567	Sentence	denotes	HeLa cells (5 × 106) were transfected with p3xFLAG-Tat or p3xFLAG empty vector (5 µg), and 48 h later were stimulated with PMA (5 min, 20 ng/ml) or left unstimulated, washed twice with DMEM and harvested at the indicated times.
T24353	5568-6016	Sentence	denotes	Nuclear and cytosolic extracts were prepared for further analysis. (A) Nuclear extracts were analysed for the p65, p50 and FLAG-Tat binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA assay kit (Cayman). (B) Nuclear and cytosolic extracts (20 µg) were separated by 12% SDS–PAGE and analysed by western blotting using the anti-p65, anti-FLAG, anti-Histone H1, anti-IκB-α and anti-Hexokinase-II antibodies.
T24354	6017-6450	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (C) IKK activity was measured in cytosolic extracts (100 µg) by using HTScan IKK kinase assay (Cell Signaling Technology). (D) HeLa cells (5 × 106) were transfected with or without p3xFLAG-Tat (5 µg); 48 h later, cells were stimulated with PMA (5 min, 20 ng/ml) or left unstimulated, washed twice with DMEM, and harvested after 240 min.
T24355	6451-6555	Sentence	denotes	Whole cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-IκB-α antibody.
T24356	6556-6687	Sentence	denotes	Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies.
T24357	6688-6784	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1).
T24358	6785-6821	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T105	0-103	Sentence	denotes	Tat enhances the NF-κB activity by hijacking IκB-α and inhibiting the post-activation turn off of NF-κB
T106	104-238	Sentence	denotes	We analysed the kinetic of NF-κB activation in single round HIV-1 infection by modulating the expression of Tat with RNA interference.
T107	239-407	Sentence	denotes	Jurkat cells were transfected with siRNA Tat, siRNA control, or left untransfected, and 24 h later cells were infected with HXB2 Env-pseudotyped NL4-3.Luc.R−E− virions.
T108	408-542	Sentence	denotes	By cytofluorimetric analysis, ∼40% of cells was siRNA-transfected and HIV-1-infected at 12-h post-infection (Supplementary Figure S1).
T109	543-765	Sentence	denotes	The expression of Tat was detected at 1-h post-infection and progressively increased up to 12 h in untransfected and siRNA control-transfected cells, while it was barely detected in siRNA Tat-transfected cells (Figure 1A).
T110	766-1060	Sentence	denotes	The LTR-dependent luciferase expression was also progressively induced at 1- to 12-h post-infection in untransfected and siRNA control-transfected cells, while it was barely observed in siRNA Tat-transfected cells, as expected for the Tat-dependent transactivation of the HIV-1 LTR (Figure 1A).
T111	1061-1070	Sentence	denotes	Figure 1.
T112	1071-1157	Sentence	denotes	Tat counteracts the post-activation turn off of NF-κB in single round HIV-1-infection.
T113	1158-1870	Sentence	denotes	Jurkat cells (5 × 107) were transfected with siRNA Tat or siRNA control (2 nmol), or left untransfected; 24 h later, cells were infected with HXB2-pseudotyped NL4-3.Luc.R−E− virions (500 ng of p24) and harvested at the indicated time. (A) Tat expression was measured in total RNA by real-time PCR, while the luciferase activity was measured in whole cell extracts. (B) Nuclear extracts (10 µg) were analysed for the p65 and p50 binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA Assay kit (Cayman). (C) The expression of p65 and IκB-α was analysed by 12% SDS–PAGE and western blotting of nuclear or cytosolic extracts (20 µg) using anti-p65 and anti-IκB-α antibodies.
T114	1871-1998	Sentence	denotes	Histone H1 and Hexokinase II were detected with specific antibodies as markers of nuclear and cytosolic extracts, respectively.
T115	1999-2216	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (mock). (D) IKK activity was measured in cytosolic extracts (100 µg) by using HTScan IKK Kinase Assay (Cell Signaling Technology).
T116	2217-2253	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T117	2254-2747	Sentence	denotes	The progressive increase in NF-κB activity, as measured by p65–p50 binding to an NF-κB consensus oligonucleotide (Figure 1B) and nuclear p65 (Figure 1C, nucleus), was observed at 1- to 3-h post-infection in both Tat-positive (no siRNA or siRNA control) and Tat-negative (siRNA Tat) cells; however, at 12-h post-infection, the p65 DNA binding and nuclear p65 persisted elevated in presence of Tat (no siRNA and siRNA control), while they dropped in absence of Tat (siRNA Tat) (Figure 1B and C).
T118	2748-2951	Sentence	denotes	IκB-α was degraded in the cytosol within 3-h post-infection and de novo synthesized at 6 h with full replenishment at 12-h post-infection in both Tat-positive and Tat-negative cells (Figure 1C, cytosol).
T119	2952-3103	Sentence	denotes	Consistently, the IKK activity was induced at 1-h post-infection, and decreased at 3-h post-infection independently of the presence of Tat (Figure 1D).
T120	3104-3369	Sentence	denotes	Similar kinetics of NF-κB activity, IKK activation and IκB-α degradation/re-synthesis were observed in single-round HIV-1 infection of PBMCs using a cocktail of Azidothymidine (AZT) and Lamivudine (3TC) as reverse transcriptase inhibitors (Supplementary Figure S2).
T121	3370-3668	Sentence	denotes	These results indicated that in single-round HIV-1 infection the NF-κB activation initially correlated with the IKK activation and IκB-α degradation independently of the presence of Tat, and it was kept elevated in presence of Tat following the decay of the IKK activity and new synthesis of IκB-α.
T122	3669-3758	Sentence	denotes	Next, we tested the single action of Tat on the kinetic of NF-κB activity induced by PMA.
T123	3759-3951	Sentence	denotes	To this end, HeLa cells were transfected with p3x-FLAG-Tat, or empty vector, stimulated with PMA for 5 min, and extensively washed to avoid the oscillatory kinetic of NF-κB activation (17,18).
T124	3952-4152	Sentence	denotes	In un-stimulated cells, the p65 DNA binding (Figure 2A) and nuclear p65 (Figure 2B, nucleus) were slightly enhanced by Tat, while the IκB-α content was not significantly affected (Figure 2B, cytosol).
T125	4153-4500	Sentence	denotes	Transient stimulation with PMA increased the NF-κB DNA binding activity and nuclear p65 at 5 min peaking at 60–120 min independently of the presence of Tat (Figure 2A and B); however, while the p65 DNA binding and nuclear p65 persisted elevated at 240-min post-treatment in Tat-positive cells, they dropped in Tat-negative cells (Figure 2A and B).
T126	4501-4725	Sentence	denotes	In addition, Tat bound to the NF-κB oligonucleotide in un-stimulated cells and its binding increased following PMA stimulation (Figure 2A), suggesting that the viral protein was a component of the NF-κB complex bound to DNA.
T127	4726-4922	Sentence	denotes	Degradation of IκB-α was progressively induced at 5–30 min, followed by de novo synthesis at 60 min with full replenishment at 240 min in both Tat-positive and negative cells (Figure 2B, cytosol).
T128	4923-5048	Sentence	denotes	Consistently, the IKK activation started at 5 min, and turned off at 30 min independently of the presence of Tat (Figure 2C).
T129	5049-5205	Sentence	denotes	These results indicated that Tat enhanced the NF-κB activity in un-stimulated and PMA-stimulated cells without affecting the IKK activity and IκB-α content.
T130	5206-5215	Sentence	denotes	Figure 2.
T131	5216-5339	Sentence	denotes	Tat enhances the NF-κB activity following PMA stimulation by associating with IκB-α and counteracting the NF-κB repression.
T132	5340-5567	Sentence	denotes	HeLa cells (5 × 106) were transfected with p3xFLAG-Tat or p3xFLAG empty vector (5 µg), and 48 h later were stimulated with PMA (5 min, 20 ng/ml) or left unstimulated, washed twice with DMEM and harvested at the indicated times.
T133	5568-6016	Sentence	denotes	Nuclear and cytosolic extracts were prepared for further analysis. (A) Nuclear extracts were analysed for the p65, p50 and FLAG-Tat binding to the NF-κB double-stranded oligonucleotide using the NF-κB Transcription Factor ELISA assay kit (Cayman). (B) Nuclear and cytosolic extracts (20 µg) were separated by 12% SDS–PAGE and analysed by western blotting using the anti-p65, anti-FLAG, anti-Histone H1, anti-IκB-α and anti-Hexokinase-II antibodies.
T134	6017-6450	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1). (C) IKK activity was measured in cytosolic extracts (100 µg) by using HTScan IKK kinase assay (Cell Signaling Technology). (D) HeLa cells (5 × 106) were transfected with or without p3xFLAG-Tat (5 µg); 48 h later, cells were stimulated with PMA (5 min, 20 ng/ml) or left unstimulated, washed twice with DMEM, and harvested after 240 min.
T135	6451-6555	Sentence	denotes	Whole cell extracts (1 mg) were immunoprecipitated with protein G-Sepharose-coupled anti-IκB-α antibody.
T136	6556-6687	Sentence	denotes	Immunocomplexes were separated by 12% SDS–PAGE and analysed by western blotting with anti-p65, anti-FLAG and anti-IκB-α antibodies.
T137	6688-6784	Sentence	denotes	Densitometry values (D) of the bands were expressed as fold increase above the control (lane 1).
T138	6785-6821	Sentence	denotes	Values (mean ± SE, n = 3) are shown.
T139	6822-6976	Sentence	denotes	As we previously found that Tat binds to the sixth ankyrin of IκB-α (50,51), we tested whether Tat counteracted the generation of the IκB-α/NF-κB complex.
T140	6977-7204	Sentence	denotes	To this end, the association of IκB-α with p65 was analysed in vivo at 0 and 240 min after short-pulse PMA, which corresponded to the time of un-stimulated condition and post-activation de novo synthesis of IκB-α, respectively.
T141	7205-7503	Sentence	denotes	Coimmunoprecipitation of IκB-α with p65 was detected at 0- and 240-min post-treatment in Tat-negative cells (Figure 2D, lanes 1 and 3), and was halved in Tat-positive cells, where Tat coimmunoprecipitated with IκB-α (Figure 2D, lanes 2 and 4), indicating that Tat competed the IκB-α binding to p65.

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T11057	0-3	Protein	denotes	Tat
T11058	35-44	Binding	denotes	hijacking
T11059	45-50	Protein	denotes	IκB-α
T11060	183-193	Regulation	denotes	modulating
T11061	198-208	Gene_expression	denotes	expression
T11062	212-215	Protein	denotes	Tat
T11063	274-283	Protein	denotes	siRNA Tat
T11073	829-836	Positive_regulation	denotes	induced
T11074	952-961	Protein	denotes	siRNA Tat
T11075	1001-1004	Protein	denotes	Tat
T11076	2313-2316	Protein	denotes	p65
T11077	2317-2320	Protein	denotes	p50
T11078	2321-2328	Binding	denotes	binding		true
T11079	2321-2328	Binding	denotes	binding		true
T11080	2391-2394	Protein	denotes	p65
T11081	2466-2469	Protein	denotes	Tat
T11082	2511-2514	Protein	denotes	Tat
T11083	2525-2534	Protein	denotes	siRNA Tat
T11084	2580-2583	Protein	denotes	p65
T11085	2588-2595	Binding	denotes	binding
T11086	2608-2611	Protein	denotes	p65
T11087	2622-2630	Positive_regulation	denotes	elevated
T11088	2622-2630	Positive_regulation	denotes	elevated
T11089	2646-2649	Protein	denotes	Tat
T11090	2691-2698	Negative_regulation	denotes	dropped
T11091	2691-2698	Negative_regulation	denotes	dropped
T11092	2702-2709	Negative_regulation	denotes	absence
T11093	2713-2716	Protein	denotes	Tat
T11094	2718-2727	Protein	denotes	siRNA Tat
T11095	2748-2753	Protein	denotes	IκB-α
T11096	2758-2766	Protein_catabolism	denotes	degraded
T11097	2820-2831	Gene_expression	denotes	synthesized
T11098	2894-2897	Protein	denotes	Tat
T11099	2911-2914	Protein	denotes	Tat
T11100	3087-3090	Protein	denotes	Tat
T11101	3159-3164	Protein	denotes	IκB-α
T11102	3165-3176	Protein_catabolism	denotes	degradation
T11103	3177-3189	Gene_expression	denotes	re-synthesis
T11104	3195-3203	Positive_regulation	denotes	observed
T11105	3195-3203	Positive_regulation	denotes	observed
T11106	3501-3506	Protein	denotes	IκB-α
T11107	3507-3518	Protein_catabolism	denotes	degradation
T11108	3552-3555	Protein	denotes	Tat
T11109	3597-3600	Protein	denotes	Tat
T11110	3649-3658	Gene_expression	denotes	synthesis
T11111	3662-3667	Protein	denotes	IκB-α
T11112	3706-3709	Protein	denotes	Tat
T11113	3814-3817	Protein	denotes	Tat
T11114	3980-3983	Protein	denotes	p65
T11115	3988-3995	Binding	denotes	binding
T11116	4020-4023	Protein	denotes	p65
T11117	4059-4067	Positive_regulation	denotes	enhanced
T11118	4059-4067	Positive_regulation	denotes	enhanced
T11119	4071-4074	Protein	denotes	Tat
T11120	4086-4091	Protein	denotes	IκB-α
T11121	4122-4130	Regulation	denotes	affected	true
T11122	4184-4193	Positive_regulation	denotes	increased
T11123	4237-4240	Protein	denotes	p65
T11124	4272-4285	Regulation	denotes	independently	true
T11125	4305-4308	Protein	denotes	Tat
T11126	4347-4350	Protein	denotes	p65
T11127	4355-4362	Binding	denotes	binding
T11128	4375-4378	Protein	denotes	p65
T11129	4389-4397	Positive_regulation	denotes	elevated
T11130	4389-4397	Positive_regulation	denotes	elevated
T11131	4427-4430	Protein	denotes	Tat
T11132	4452-4459	Negative_regulation	denotes	dropped
T11133	4452-4459	Negative_regulation	denotes	dropped
T11134	4463-4466	Protein	denotes	Tat
T11135	4467-4475	Negative_regulation	denotes	negative
T11136	4514-4517	Protein	denotes	Tat
T11137	4518-4523	Binding	denotes	bound
T11138	4584-4591	Binding	denotes	binding
T11139	4592-4601	Positive_regulation	denotes	increased
T11140	4726-4737	Protein_catabolism	denotes	Degradation
T11141	4741-4746	Protein	denotes	IκB-α
T11142	4765-4772	Positive_regulation	denotes	induced
T11143	4786-4794	Positive_regulation	denotes	followed
T11144	4806-4815	Gene_expression	denotes	synthesis
T11145	4869-4872	Protein	denotes	Tat
T11146	5032-5035	Protein	denotes	Tat
T11147	5078-5081	Protein	denotes	Tat
T11148	5160-5169	Regulation	denotes	affecting	true
T11149	5191-5196	Protein	denotes	IκB-α
T11150	6850-6853	Protein	denotes	Tat
T11151	6854-6859	Binding	denotes	binds
T11152	6867-6880	Entity	denotes	sixth ankyrin
T11153	6884-6889	Protein	denotes	IκB-α
T11154	6917-6920	Protein	denotes	Tat
T11155	6921-6933	Negative_regulation	denotes	counteracted		true
T11156	6938-6948	Binding	denotes	generation
T11157	6956-6961	Protein	denotes	IκB-α
T11158	6994-7005	Binding	denotes	association		true
T11159	7009-7014	Protein	denotes	IκB-α
T11160	7020-7023	Protein	denotes	p65
T11161	7171-7180	Gene_expression	denotes	synthesis
T11162	7184-7189	Protein	denotes	IκB-α
T11163	7205-7226	Binding	denotes	Coimmunoprecipitation
T11164	7230-7235	Protein	denotes	IκB-α
T11165	7241-7244	Protein	denotes	p65
T11166	7294-7297	Protein	denotes	Tat
T11167	7349-7355	Negative_regulation	denotes	halved
T11168	7359-7362	Protein	denotes	Tat
T11169	7385-7388	Protein	denotes	Tat
T11170	7389-7409	Binding	denotes	coimmunoprecipitated
T11171	7415-7420	Protein	denotes	IκB-α
T11172	7465-7468	Protein	denotes	Tat
T11173	7469-7477	Negative_regulation	denotes	competed
T11174	7482-7487	Protein	denotes	IκB-α
T11175	7488-7495	Binding	denotes	binding
T11176	7499-7502	Protein	denotes	p65
T24282	1071-1074	Protein	denotes	Tat
T24283	1203-1212	Protein	denotes	siRNA Tat
T24284	1351-1354	Protein	denotes	p24
T24285	1397-1400	Protein	denotes	Tat
T24286	1401-1411	Gene_expression	denotes	expression		true
T24287	1466-1476	Protein	denotes	luciferase
T24288	1574-1577	Protein	denotes	p65
T24289	1582-1585	Protein	denotes	p50
T24290	1586-1593	Binding	denotes	binding		true
T24291	1586-1593	Binding	denotes	binding		true
T24292	1710-1720	Gene_expression	denotes	expression		true
T24293	1710-1720	Gene_expression	denotes	expression		true
T24294	1724-1727	Protein	denotes	p65
T24295	1732-1737	Protein	denotes	IκB-α
T24296	1886-1899	Protein	denotes	Hexokinase II
T25060	5216-5219	Protein	denotes	Tat
T25061	5277-5288	Binding	denotes	associating
T25062	5294-5299	Protein	denotes	IκB-α
T25063	5391-5394	Protein	denotes	Tat
T25064	5678-5681	Protein	denotes	p65
T25065	5683-5686	Protein	denotes	p50
T25066	5691-5699	Protein	denotes	FLAG-Tat
T25067	5700-5707	Binding	denotes	binding		true
T25068	5700-5707	Binding	denotes	binding		true
T25069	5700-5707	Binding	denotes	binding		true
T25070	6303-6306	Protein	denotes	Tat
T25071	6507-6516	Protein	denotes	protein G
T11064	368-371	Protein	denotes	Env
T11065	547-557	Gene_expression	denotes	expression
T11066	561-564	Protein	denotes	Tat
T11067	618-627	Positive_regulation	denotes	increased
T11068	706-721	Negative_regulation	denotes	barely detected
T11069	725-734	Protein	denotes	siRNA Tat
T11070	774-783	Positive_regulation	denotes	dependent
T11071	784-794	Protein	denotes	luciferase
T11072	795-805	Gene_expression	denotes	expression
R9042	T11057	T11058	themeOf	Tat,hijacking
R9044	T11059	T11058	themeOf	IκB-α,hijacking
R9046	T11061	T11060	themeOf	expression,modulating
R9047	T11062	T11061	themeOf	Tat,expression
R9050	T11065	T11067	themeOf	expression,increased
R9051	T11065	T11068	themeOf	expression,barely detected
R9052	T11066	T11065	themeOf	Tat,expression
R9056	T11069	T11068	causeOf	siRNA Tat,barely detected
R9058	T11070	T11073	themeOf	dependent,induced
R9059	T11071	T11072	themeOf	luciferase,expression
R9060	T11072	T11070	themeOf	expression,dependent
R9065	T11076	T11079	themeOf	p65,binding
R9067	T11077	T11078	themeOf	p50,binding
R9071	T11084	T11085	themeOf	p65,binding
R9072	T11085	T11088	themeOf	binding,elevated
R9073	T11085	T11090	themeOf	binding,dropped
R9074	T11086	T11087	themeOf	p65,elevated
R9075	T11086	T11091	themeOf	p65,dropped
R9078	T11089	T11088	causeOf	Tat,elevated
R9079	T11089	T11087	causeOf	Tat,elevated
R9080	T11092	T11090	causeOf	absence,dropped
R9081	T11092	T11091	causeOf	absence,dropped
R9082	T11093	T11092	themeOf	Tat,absence
R9084	T11095	T11096	themeOf	IκB-α,degraded
R9085	T11095	T11097	themeOf	IκB-α,synthesized
R9090	T11101	T11102	themeOf	IκB-α,degradation
R9091	T11101	T11103	themeOf	IκB-α,re-synthesis
R9092	T11102	T11105	themeOf	degradation,observed
R9093	T11103	T11104	themeOf	re-synthesis,observed
R9095	T11106	T11107	themeOf	IκB-α,degradation
R9096	T11111	T11110	themeOf	IκB-α,synthesis
R9098	T11114	T11115	themeOf	p65,binding
R9100	T11115	T11117	themeOf	binding,enhanced
R9102	T11116	T11118	themeOf	p65,enhanced
R9104	T11119	T11117	causeOf	Tat,enhanced
R9105	T11119	T11118	causeOf	Tat,enhanced
R9106	T11119	T11121	causeOf	Tat,affected
R9107	T11120	T11121	themeOf	IκB-α,affected
R9108	T11122	T11124	themeOf	increased,independently
R9110	T11123	T11122	themeOf	p65,increased
R9111	T11125	T11124	causeOf	Tat,independently
R9112	T11126	T11127	themeOf	p65,binding
R9113	T11127	T11129	themeOf	binding,elevated
R9114	T11127	T11132	themeOf	binding,dropped
R9116	T11128	T11130	themeOf	p65,elevated
R9117	T11128	T11133	themeOf	p65,dropped
R9118	T11131	T11129	causeOf	Tat,elevated
R9119	T11131	T11130	causeOf	Tat,elevated
R9121	T11134	T11135	themeOf	Tat,negative
R9123	T11135	T11132	causeOf	negative,dropped
R9124	T11135	T11133	causeOf	negative,dropped
R9125	T11136	T11137	themeOf	Tat,bound
R9126	T11136	T11138	themeOf	Tat,binding
R9127	T11138	T11139	themeOf	binding,increased
R9129	T11140	T11142	themeOf	Degradation,induced
R9131	T11141	T11140	themeOf	IκB-α,Degradation
R9132	T11141	T11144	themeOf	IκB-α,synthesis
R9133	T11142	T11143	causeOf	induced,followed
R9134	T11144	T11143	themeOf	synthesis,followed
R9136	T11147	T11148	causeOf	Tat,affecting
R9138	T11149	T11148	themeOf	IκB-α,affecting
R9139	T11150	T11151	themeOf	Tat,binds
R9141	T11152	T11151	themeOf	sixth ankyrin,binds
R9142	T11152	T11153	partOf	sixth ankyrin,IκB-α
R9143	T11154	T11155	causeOf	Tat,counteracted
R9145	T11156	T11155	themeOf	generation,counteracted
R9146	T11157	T11156	themeOf	IκB-α,generation
R9147	T11159	T11158	themeOf	IκB-α,association
R9148	T11160	T11158	themeOf	p65,association
R9150	T11162	T11161	themeOf	IκB-α,synthesis
R9151	T11163	T11167	themeOf	Coimmunoprecipitation,halved
R9152	T11164	T11163	themeOf	IκB-α,Coimmunoprecipitation
R9153	T11165	T11163	themeOf	p65,Coimmunoprecipitation
R9157	T11168	T11167	causeOf	Tat,halved
R9158	T11169	T11170	themeOf	Tat,coimmunoprecipitated
R9160	T11171	T11170	themeOf	IκB-α,coimmunoprecipitated
R9161	T11172	T11173	causeOf	Tat,competed
R9162	T11174	T11175	themeOf	IκB-α,binding
R9164	T11175	T11173	themeOf	binding,competed
R9165	T11176	T11175	themeOf	p65,binding
R19331	T24285	T24286	themeOf	Tat,expression
R19332	T24288	T24290	themeOf	p65,binding
R19333	T24289	T24291	themeOf	p50,binding
R19334	T24294	T24292	themeOf	p65,expression
R19335	T24295	T24293	themeOf	IκB-α,expression
R20000	T25060	T25061	themeOf	Tat,associating
R20001	T25062	T25061	themeOf	IκB-α,associating
R20002	T25064	T25068	themeOf	p65,binding
R20003	T25065	T25067	themeOf	p50,binding
R20004	T25066	T25069	themeOf	FLAG-Tat,binding

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T11177	0-3	Protein	denotes	Tat
T11178	17-22	Protein	denotes	NF-κB
T11179	45-50	Protein	denotes	IκB-α
T11180	98-103	Protein	denotes	NF-κB
T11181	4-12	Positive_regulation	denotes	enhances
T11182	35-44	Positive_regulation	denotes	hijacking
T11183	131-136	Protein	denotes	NF-κB
T11184	212-215	Protein	denotes	Tat
T11185	137-147	Positive_regulation	denotes	activation
T11186	198-208	Gene_expression	denotes	expression
T11187	183-193	Regulation	denotes	modulating
T11188	274-290	Protein	denotes	siRNA Tat, siRNA
T11189	363-371	Protein	denotes	HXB2 Env
T11190	561-564	Protein	denotes	Tat
T11191	731-734	Protein	denotes	Tat
T11192	547-557	Gene_expression	denotes	expression
T11193	618-627	Positive_regulation	denotes	increased
T11194	735-746	Gene_expression	denotes	transfected
T11195	735-746	Positive_regulation	denotes	transfected
T11196	784-794	Protein	denotes	luciferase
T11197	958-961	Protein	denotes	Tat
T11198	1001-1004	Protein	denotes	Tat
T11199	795-805	Gene_expression	denotes	expression
T11200	829-836	Positive_regulation	denotes	induced
T11201	962-973	Gene_expression	denotes	transfected
T11202	962-973	Positive_regulation	denotes	transfected
T11203	2282-2287	Protein	denotes	NF-κB
T11204	2313-2316	Protein	denotes	p65
T11205	2317-2320	Protein	denotes	p50
T11206	2466-2469	Protein	denotes	Tat
T11207	2580-2587	Protein	denotes	p65 DNA
T11208	2646-2649	Protein	denotes	Tat
T11209	2270-2278	Positive_regulation	denotes	increase
T11210	2321-2328	Binding	denotes	binding
T11211	2321-2328	Binding	denotes	binding
T11212	2443-2457	Localization	denotes	post-infection
T11213	2470-2478	Gene_expression	denotes	positive
T11214	2588-2595	Binding	denotes	binding
T11215	2622-2630	Positive_regulation	denotes	elevated
T11216	2634-2642	Localization	denotes	presence
T11217	2622-2630	Positive_regulation	denotes	elevated
T11218	2748-2753	Protein	denotes	IκB-α
T11219	2894-2897	Protein	denotes	Tat
T11220	2911-2914	Protein	denotes	Tat
T11221	2758-2766	Protein_catabolism	denotes	degraded
T11222	2898-2906	Gene_expression	denotes	positive
T11223	2915-2923	Gene_expression	denotes	negative
T11224	2915-2923	Gene_expression	denotes	negative
T11225	2970-2973	Protein	denotes	IKK
T11226	2987-2994	Positive_regulation	denotes	induced
T11227	3022-3031	Negative_regulation	denotes	decreased
T11228	3124-3129	Protein	denotes	NF-κB
T11229	3140-3143	Protein	denotes	IKK
T11230	3159-3164	Protein	denotes	IκB-α
T11231	3310-3331	Protein	denotes	reverse transcriptase
T11232	3144-3154	Positive_regulation	denotes	activation
T11233	3165-3189	Gene_expression	denotes	degradation/re-synthesis
T11234	3165-3189	Gene_expression	denotes	degradation/re-synthesis
T11235	3144-3154	Positive_regulation	denotes	activation
T11236	3144-3154	Positive_regulation	denotes	activation
T11237	3435-3440	Protein	denotes	NF-κB
T11238	3482-3485	Protein	denotes	IKK
T11239	3501-3506	Protein	denotes	IκB-α
T11240	3552-3555	Protein	denotes	Tat
T11241	3597-3600	Protein	denotes	Tat
T11242	3628-3631	Protein	denotes	IKK
T11243	3662-3667	Protein	denotes	IκB-α
T11244	3441-3451	Positive_regulation	denotes	activation
T11245	3486-3496	Positive_regulation	denotes	activation
T11246	3507-3518	Protein_catabolism	denotes	degradation
T11247	3649-3658	Gene_expression	denotes	synthesis
T11248	3486-3496	Positive_regulation	denotes	activation
T11249	3573-3581	Positive_regulation	denotes	elevated
T11250	3706-3709	Protein	denotes	Tat
T11251	3728-3733	Protein	denotes	NF-κB
T11252	3743-3750	Positive_regulation	denotes	induced
T11253	3805-3808	Protein	denotes	p3x
T11254	3809-3813	Protein	denotes	FLAG
T11255	3814-3817	Protein	denotes	Tat
T11256	3926-3931	Protein	denotes	NF-κB
T11257	3932-3942	Positive_regulation	denotes	activation
T11258	3980-3987	Protein	denotes	p65 DNA
T11259	4071-4074	Protein	denotes	Tat
T11260	4086-4091	Protein	denotes	IκB-α
T11261	3988-3995	Binding	denotes	binding
T11262	4059-4067	Positive_regulation	denotes	enhanced
T11263	4122-4130	Regulation	denotes	affected
T11264	4198-4203	Protein	denotes	NF-κB
T11265	4229-4249	Protein	denotes	nuclear p65 at 5 min
T11266	4347-4354	Protein	denotes	p65 DNA
T11267	4463-4466	Protein	denotes	Tat
T11268	4467-4475	Gene_expression	denotes	negative
T11269	4514-4517	Protein	denotes	Tat
T11270	4531-4552	Protein	denotes	NF-κB oligonucleotide
T11271	4698-4711	Protein	denotes	NF-κB complex
T11272	4518-4523	Binding	denotes	bound
T11273	4518-4523	Binding	denotes	bound
T11274	4584-4591	Binding	denotes	binding
T11275	4592-4601	Positive_regulation	denotes	increased
T11276	4681-4690	Gene_expression	denotes	component
T11277	4712-4717	Binding	denotes	bound
T11278	4741-4746	Protein	denotes	IκB-α
T11279	4869-4872	Protein	denotes	Tat
T11280	4726-4737	Protein_catabolism	denotes	Degradation
T11281	4765-4772	Positive_regulation	denotes	induced
T11282	4873-4881	Gene_expression	denotes	positive
T11283	4941-4944	Protein	denotes	IKK
T11284	4945-4955	Positive_regulation	denotes	activation
T11285	5078-5081	Protein	denotes	Tat
T11286	5095-5100	Protein	denotes	NF-κB
T11287	5174-5177	Protein	denotes	IKK
T11288	5191-5196	Protein	denotes	IκB-α
T11289	5082-5090	Positive_regulation	denotes	enhanced
T11290	5160-5169	Regulation	denotes	affecting
T11291	5160-5169	Regulation	denotes	affecting
T11292	6850-6853	Protein	denotes	Tat
T11293	6873-6880	Protein	denotes	ankyrin
T11294	6884-6889	Protein	denotes	IκB-α
T11295	6917-6920	Protein	denotes	Tat
T11296	6956-6961	Protein	denotes	IκB-α
T11297	6962-6975	Protein	denotes	NF-κB complex
T11298	6854-6859	Binding	denotes	binds
T11299	6854-6859	Binding	denotes	binds
T11300	6921-6933	Binding	denotes	counteracted
T11301	6921-6933	Binding	denotes	counteracted
T11302	6938-6948	Positive_regulation	denotes	generation
T11303	6938-6948	Positive_regulation	denotes	generation
T11304	7009-7014	Protein	denotes	IκB-α
T11305	7020-7023	Protein	denotes	p65
T11306	7184-7189	Protein	denotes	IκB-α
T11307	6994-7005	Binding	denotes	association
T11308	7171-7180	Gene_expression	denotes	synthesis
T11309	7230-7235	Protein	denotes	IκB-α
T11310	7241-7244	Protein	denotes	p65
T11311	7294-7297	Protein	denotes	Tat
T11312	7359-7362	Protein	denotes	Tat
T11313	7385-7388	Protein	denotes	Tat
T11314	7415-7420	Protein	denotes	IκB-α
T11315	7465-7468	Protein	denotes	Tat
T11316	7482-7487	Protein	denotes	IκB-α
T11317	7499-7502	Protein	denotes	p65
T11318	7205-7226	Binding	denotes	Coimmunoprecipitation
T11319	7298-7306	Gene_expression	denotes	negative
T11320	7363-7371	Gene_expression	denotes	positive
T11321	7389-7409	Binding	denotes	coimmunoprecipitated
T11322	7389-7409	Binding	denotes	coimmunoprecipitated
T11323	7469-7477	Binding	denotes	competed
T11324	7488-7495	Binding	denotes	binding
T24297	1071-1074	Protein	denotes	Tat
T24298	1119-1124	Protein	denotes	NF-κB
T24299	1203-1221	Protein	denotes	siRNA Tat or siRNA
T24300	1351-1354	Protein	denotes	p24
T24301	1397-1400	Protein	denotes	Tat
T24302	1466-1476	Protein	denotes	luciferase
T24303	1401-1411	Gene_expression	denotes	expression
T24304	1574-1577	Protein	denotes	p65
T24305	1582-1585	Protein	denotes	p50
T24306	1601-1606	Protein	denotes	NF-κB
T24307	1649-1654	Protein	denotes	NF-κB
T24308	1724-1727	Protein	denotes	p65
T24309	1732-1737	Protein	denotes	IκB-α
T24310	1840-1843	Protein	denotes	p65
T24311	1848-1869	Protein	denotes	anti-IκB-α antibodies
T24312	1710-1720	Gene_expression	denotes	expression
T24313	1710-1720	Gene_expression	denotes	expression
T24314	1871-1899	Protein	denotes	Histone H1 and Hexokinase II
T24315	2098-2101	Protein	denotes	IKK
T24316	2164-2174	Protein	denotes	HTScan IKK
T25072	5216-5219	Protein	denotes	Tat
T25073	5233-5238	Protein	denotes	NF-κB
T25074	5294-5299	Protein	denotes	IκB-α
T25075	5322-5327	Protein	denotes	NF-κB
T25076	5277-5288	Binding	denotes	associating
T25077	5328-5338	Negative_regulation	denotes	repression
T25078	5383-5394	Protein	denotes	p3xFLAG-Tat
T25079	5678-5681	Protein	denotes	p65
T25080	5683-5686	Protein	denotes	p50
T25081	5691-5695	Protein	denotes	FLAG
T25082	5696-5699	Protein	denotes	Tat
T25083	5715-5720	Protein	denotes	NF-κB
T25084	5763-5768	Protein	denotes	NF-κB
T25085	5938-5941	Protein	denotes	p65
T25086	5943-5952	Protein	denotes	anti-FLAG
T25087	5954-5981	Protein	denotes	anti-Histone H1, anti-IκB-α
T25088	5986-6015	Protein	denotes	anti-Hexokinase-II antibodies
T25089	6184-6194	Protein	denotes	HTScan IKK
T25090	6507-6554	Protein	denotes	protein G-Sepharose-coupled anti-IκB-α antibody
T25091	6646-6649	Protein	denotes	p65
T25092	6651-6660	Protein	denotes	anti-FLAG
T25093	6665-6686	Protein	denotes	anti-IκB-α antibodies
R9171	T11251	T11252	themeOf	NF-κB,induced
R9172	T11177	T11181	causeOf	Tat,enhances
R9173	T11178	T11181	themeOf	NF-κB,enhances
R9174	T11179	T11182	themeOf	IκB-α,hijacking
R9175	T11256	T11257	themeOf	NF-κB,activation
R9176	T11183	T11185	themeOf	NF-κB,activation
R9177	T11184	T11186	themeOf	Tat,expression
R9178	T11186	T11187	themeOf	expression,modulating
R9179	T11258	T11261	themeOf	p65 DNA,binding
R9180	T11190	T11192	themeOf	Tat,expression
R9181	T11259	T11262	causeOf	Tat,enhanced
R9182	T11191	T11194	themeOf	Tat,transfected
R9183	T11192	T11193	themeOf	expression,increased
R9184	T11260	T11263	themeOf	IκB-α,affected
R9185	T11194	T11195	themeOf	transfected,transfected
R9186	T11261	T11262	themeOf	binding,enhanced
R9187	T11196	T11199	themeOf	luciferase,expression
R9188	T11197	T11201	themeOf	Tat,transfected
R9189	T11267	T11268	themeOf	Tat,negative
R9190	T11199	T11200	themeOf	expression,induced
R9191	T11201	T11202	themeOf	transfected,transfected
R9192	T11203	T11209	themeOf	NF-κB,increase
R9193	T11203	T11212	themeOf	NF-κB,post-infection
R9194	T11204	T11210	themeOf	p65,binding
R9195	T11204	T11211	themeOf	p65,binding
R9196	T11205	T11211	themeOf	p50,binding
R9197	T11206	T11213	themeOf	Tat,positive
R9198	T11207	T11214	themeOf	p65 DNA,binding
R9199	T11208	T11216	themeOf	Tat,presence
R9200	T11269	T11272	themeOf	Tat,bound
R9201	T11214	T11215	themeOf	binding,elevated
R9202	T11269	T11273	themeOf	Tat,bound
R9203	T11269	T11274	themeOf	Tat,binding
R9204	T11216	T11217	themeOf	presence,elevated
R9205	T11270	T11273	themeOf	NF-κB oligonucleotide,bound
R9206	T11218	T11221	themeOf	IκB-α,degraded
R9207	T11219	T11222	themeOf	Tat,positive
R9208	T11219	T11223	themeOf	Tat,negative
R9209	T11271	T11276	themeOf	NF-κB complex,component
R9210	T11220	T11224	themeOf	Tat,negative
R9211	T11271	T11277	themeOf	NF-κB complex,bound
R9212	T11274	T11275	themeOf	binding,increased
R9213	T11225	T11226	themeOf	IKK,induced
R9214	T11225	T11227	themeOf	IKK,decreased
R9215	T11228	T11233	themeOf	NF-κB,degradation/re-synthesis
R9216	T11229	T11232	themeOf	IKK,activation
R9217	T11278	T11280	themeOf	IκB-α,Degradation
R9218	T11230	T11234	themeOf	IκB-α,degradation/re-synthesis
R9219	T11233	T11235	themeOf	degradation/re-synthesis,activation
R9220	T11234	T11236	themeOf	degradation/re-synthesis,activation
R9221	T11279	T11282	themeOf	Tat,positive
R9222	T11237	T11244	themeOf	NF-κB,activation
R9223	T11238	T11245	themeOf	IKK,activation
R9224	T11280	T11281	themeOf	Degradation,induced
R9225	T11239	T11246	themeOf	IκB-α,degradation
R9226	T11283	T11284	themeOf	IKK,activation
R9227	T11243	T11247	themeOf	IκB-α,synthesis
R9228	T11246	T11248	themeOf	degradation,activation
R9229	T11285	T11289	causeOf	Tat,enhanced
R9230	T11247	T11249	themeOf	synthesis,elevated
R9231	T11286	T11289	themeOf	NF-κB,enhanced
R9232	T11287	T11290	themeOf	IKK,affecting
R9233	T11288	T11291	themeOf	IκB-α,affecting
R9234	T11292	T11298	themeOf	Tat,binds
R9235	T11292	T11299	themeOf	Tat,binds
R9236	T11293	T11298	themeOf	ankyrin,binds
R9237	T11294	T11299	themeOf	IκB-α,binds
R9238	T11295	T11300	themeOf	Tat,counteracted
R9239	T11295	T11301	themeOf	Tat,counteracted
R9240	T11296	T11302	themeOf	IκB-α,generation
R9241	T11297	T11301	themeOf	NF-κB complex,counteracted
R9242	T11297	T11303	themeOf	NF-κB complex,generation
R9243	T11304	T11307	themeOf	IκB-α,association
R9244	T11305	T11307	themeOf	p65,association
R9245	T11306	T11308	themeOf	IκB-α,synthesis
R9246	T11309	T11318	themeOf	IκB-α,Coimmunoprecipitation
R9247	T11310	T11318	themeOf	p65,Coimmunoprecipitation
R9248	T11311	T11319	themeOf	Tat,negative
R9249	T11312	T11320	themeOf	Tat,positive
R9250	T11313	T11321	themeOf	Tat,coimmunoprecipitated
R9251	T11313	T11322	themeOf	Tat,coimmunoprecipitated
R9252	T11314	T11322	themeOf	IκB-α,coimmunoprecipitated
R9253	T11315	T11323	themeOf	Tat,competed
R9254	T11316	T11324	themeOf	IκB-α,binding
R9255	T11317	T11324	themeOf	p65,binding
R19336	T24301	T24303	themeOf	Tat,expression
R19337	T24308	T24312	themeOf	p65,expression
R19338	T24309	T24313	themeOf	IκB-α,expression
R20005	T25074	T25076	themeOf	IκB-α,associating
R20006	T25075	T25077	themeOf	NF-κB,repression

test2

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T8360	0-3	Protein	denotes	Tat
T8361	35-44	Binding	denotes	hijacking
T8362	45-50	Protein	denotes	IκB-α
T8363	183-193	Regulation	denotes	modulating
T8364	198-208	Gene_expression	denotes	expression
T8365	212-215	Protein	denotes	Tat
T8366	274-283	Protein	denotes	siRNA Tat
T8367	368-371	Protein	denotes	Env
T8368	547-557	Gene_expression	denotes	expression
T8369	561-564	Protein	denotes	Tat
T8370	618-627	Positive_regulation	denotes	increased		true
T8371	725-734	Protein	denotes	siRNA Tat		true
T8372	774-783	Regulation	denotes	dependent
T8373	784-794	Protein	denotes	luciferase
T8374	795-805	Gene_expression	denotes	expression
T8375	829-836	Positive_regulation	denotes	induced
T8376	952-961	Protein	denotes	siRNA Tat
T8377	1001-1004	Protein	denotes	Tat
T8378	2313-2316	Protein	denotes	p65
T8379	2317-2320	Protein	denotes	p50
T8380	2321-2328	Binding	denotes	binding
T8381	2391-2394	Protein	denotes	p65
T8382	2466-2469	Protein	denotes	Tat
T8383	2511-2514	Protein	denotes	Tat
T8384	2515-2523	Negative_regulation	denotes	negative
T8385	2525-2534	Protein	denotes	siRNA Tat
T8386	2580-2583	Protein	denotes	p65
T8387	2588-2595	Binding	denotes	binding
T8388	2600-2607	Entity	denotes	nuclear
T8389	2608-2611	Protein	denotes	p65	true
T8390	2622-2630	Positive_regulation	denotes	elevated
T8391	2646-2649	Protein	denotes	Tat	true
T8392	2702-2709	Negative_regulation	denotes	absence
T8393	2713-2716	Protein	denotes	Tat
T8394	2718-2727	Protein	denotes	siRNA Tat
T8395	2748-2753	Protein	denotes	IκB-α
T8396	2758-2766	Protein_catabolism	denotes	degraded
T8397	2894-2897	Protein	denotes	Tat
T8398	2911-2914	Protein	denotes	Tat
T8399	3087-3090	Protein	denotes	Tat
T8400	3159-3164	Protein	denotes	IκB-α
T8401	3165-3176	Protein_catabolism	denotes	degradation
T8402	3177-3189	Gene_expression	denotes	re-synthesis
T8403	3501-3506	Protein	denotes	IκB-α
T8404	3507-3518	Protein_catabolism	denotes	degradation	true
T8405	3552-3555	Protein	denotes	Tat
T8406	3573-3581	Positive_regulation	denotes	elevated		true
T8407	3597-3600	Protein	denotes	Tat
T8408	3601-3610	Positive_regulation	denotes	following		true
T8409	3649-3658	Gene_expression	denotes	synthesis
T8410	3662-3667	Protein	denotes	IκB-α
T23883	1071-1074	Protein	denotes	Tat		true
T23884	1203-1212	Protein	denotes	siRNA Tat		true
T23885	1351-1354	Protein	denotes	p24		true
T23886	1397-1400	Protein	denotes	Tat		true
T23887	1401-1411	Gene_expression	denotes	expression		true
T23888	1466-1476	Protein	denotes	luciferase
T23889	1574-1577	Protein	denotes	p65
T23890	1582-1585	Protein	denotes	p50
T23891	1586-1593	Binding	denotes	binding
T23892	1710-1720	Gene_expression	denotes	expression
T23893	1724-1727	Protein	denotes	p65
T23894	1732-1737	Protein	denotes	IκB-α
T23895	1886-1899	Protein	denotes	Hexokinase II
T24329	5216-5219	Protein	denotes	Tat
T24330	5277-5288	Binding	denotes	associating		true
T24331	5294-5299	Protein	denotes	IκB-α		true
T24332	5391-5394	Protein	denotes	Tat		true
T24333	5678-5681	Protein	denotes	p65
T24334	5683-5686	Protein	denotes	p50
T24335	5691-5699	Protein	denotes	FLAG-Tat
T24336	5700-5707	Binding	denotes	binding
T24337	6303-6306	Protein	denotes	Tat
T24338	6507-6516	Protein	denotes	protein G
R6936	T8360	T8361	themeOf	Tat,hijacking
R6937	T8362	T8361	themeOf	IκB-α,hijacking
R6938	T8364	T8363	themeOf	expression,modulating
R6939	T8365	T8364	themeOf	Tat,expression
R6940	T8368	T8370	themeOf	expression,increased
R6941	T8369	T8368	themeOf	Tat,expression
R6942	T8372	T8375	themeOf	dependent,induced
R6943	T8373	T8374	themeOf	luciferase,expression
R6944	T8374	T8375	themeOf	expression,induced
R6945	T8374	T8372	themeOf	expression,dependent
R6946	T8376	T8375	causeOf	siRNA Tat,induced
R6947	T8378	T8380	themeOf	p65,binding
R6948	T8379	T8380	themeOf	p50,binding
R6949	T8383	T8384	themeOf	Tat,negative
R6950	T8386	T8387	themeOf	p65,binding
R6951	T8387	T8390	themeOf	binding,elevated
R6952	T8389	T8390	themeOf	p65,elevated
R6953	T8391	T8390	causeOf	Tat,elevated
R6954	T8392	T8390	causeOf	absence,elevated
R6955	T8393	T8392	themeOf	Tat,absence
R6956	T8395	T8396	themeOf	IκB-α,degraded
R6957	T8400	T8401	themeOf	IκB-α,degradation
R6958	T8400	T8402	themeOf	IκB-α,re-synthesis
R6959	T8403	T8404	themeOf	IκB-α,degradation
R6960	T8407	T8406	causeOf	Tat,elevated
R6961	T8410	T8409	themeOf	IκB-α,synthesis
R19057	T23886	T23887	themeOf	Tat,expression
R19058	T23889	T23891	themeOf	p65,binding
R19059	T23890	T23891	themeOf	p50,binding
R19060	T23893	T23892	themeOf	p65,expression
R19061	T23894	T23892	themeOf	IκB-α,expression
R19344	T24329	T24330	themeOf	Tat,associating
R19345	T24331	T24330	themeOf	IκB-α,associating
R19346	T24333	T24336	themeOf	p65,binding
R19347	T24334	T24336	themeOf	p50,binding
R19348	T24335	T24336	themeOf	FLAG-Tat,binding

bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Negation	Speculation
T8422	618-627	Positive_regulation	denotes	increased
T8423	706-721	Negative_regulation	denotes	barely detected
T8424	725-734	Protein	denotes	siRNA Tat
T8425	774-783	Positive_regulation	denotes	dependent
T8426	784-794	Protein	denotes	luciferase
T8427	795-805	Gene_expression	denotes	expression
T8428	829-836	Positive_regulation	denotes	induced
T8429	952-961	Protein	denotes	siRNA Tat
T8430	1001-1004	Protein	denotes	Tat
T8431	2313-2316	Protein	denotes	p65
T8432	2317-2320	Protein	denotes	p50
T8433	2321-2328	Binding	denotes	binding		true
T8434	2321-2328	Binding	denotes	binding		true
T8435	2391-2394	Protein	denotes	p65
T8436	2466-2469	Protein	denotes	Tat
T8437	2511-2514	Protein	denotes	Tat
T8438	2525-2534	Protein	denotes	siRNA Tat
T8439	2580-2583	Protein	denotes	p65
T8440	2588-2595	Binding	denotes	binding
T8441	2608-2611	Protein	denotes	p65
T8442	2622-2630	Positive_regulation	denotes	elevated
T8443	2622-2630	Positive_regulation	denotes	elevated
T8444	2646-2649	Protein	denotes	Tat
T8445	2691-2698	Negative_regulation	denotes	dropped
T8446	2691-2698	Negative_regulation	denotes	dropped
T8447	2702-2709	Negative_regulation	denotes	absence
T8448	2713-2716	Protein	denotes	Tat
T8449	2718-2727	Protein	denotes	siRNA Tat
T8450	2748-2753	Protein	denotes	IκB-α
T8451	2758-2766	Protein_catabolism	denotes	degraded
T8452	2820-2831	Gene_expression	denotes	synthesized
T8453	2894-2897	Protein	denotes	Tat
T8454	2911-2914	Protein	denotes	Tat
T8455	3087-3090	Protein	denotes	Tat
T8456	3159-3164	Protein	denotes	IκB-α
T8457	3165-3176	Protein_catabolism	denotes	degradation
T8458	3177-3189	Gene_expression	denotes	re-synthesis
T8459	3195-3203	Positive_regulation	denotes	observed
T8485	4389-4397	Positive_regulation	denotes	elevated
T8486	4427-4430	Protein	denotes	Tat
T8487	4452-4459	Negative_regulation	denotes	dropped
T8488	4452-4459	Negative_regulation	denotes	dropped
T8489	4463-4466	Protein	denotes	Tat
T8490	4467-4475	Negative_regulation	denotes	negative
T8491	4514-4517	Protein	denotes	Tat
T8492	4518-4523	Binding	denotes	bound
T8493	4584-4591	Binding	denotes	binding
T8494	4592-4601	Positive_regulation	denotes	increased
T8495	4726-4737	Protein_catabolism	denotes	Degradation
T8496	4741-4746	Protein	denotes	IκB-α
T8497	4765-4772	Positive_regulation	denotes	induced
T8498	4786-4794	Positive_regulation	denotes	followed
T8499	4806-4815	Gene_expression	denotes	synthesis
T8500	4869-4872	Protein	denotes	Tat
T8501	5032-5035	Protein	denotes	Tat
T8502	5078-5081	Protein	denotes	Tat
T8503	5160-5169	Regulation	denotes	affecting	true
T8504	5191-5196	Protein	denotes	IκB-α
T8505	6850-6853	Protein	denotes	Tat
T8506	6854-6859	Binding	denotes	binds
T8507	6867-6880	Entity	denotes	sixth ankyrin
T8508	6884-6889	Protein	denotes	IκB-α
T8509	6917-6920	Protein	denotes	Tat
T8510	6921-6933	Negative_regulation	denotes	counteracted		true
T8511	6938-6948	Binding	denotes	generation
T8512	6956-6961	Protein	denotes	IκB-α
T8513	6994-7005	Binding	denotes	association		true
T8514	7009-7014	Protein	denotes	IκB-α
T8515	7020-7023	Protein	denotes	p65
T8516	7171-7180	Gene_expression	denotes	synthesis
T8517	7184-7189	Protein	denotes	IκB-α
T8518	7205-7226	Binding	denotes	Coimmunoprecipitation
T8519	7230-7235	Protein	denotes	IκB-α
T8520	7241-7244	Protein	denotes	p65
T8521	7294-7297	Protein	denotes	Tat
T8522	7349-7355	Negative_regulation	denotes	halved
T8523	7359-7362	Protein	denotes	Tat
T8524	7385-7388	Protein	denotes	Tat
T8525	7389-7409	Binding	denotes	coimmunoprecipitated
T8526	7415-7420	Protein	denotes	IκB-α
T8527	7465-7468	Protein	denotes	Tat
T8528	7469-7477	Negative_regulation	denotes	competed
T8529	7482-7487	Protein	denotes	IκB-α
T8530	7488-7495	Binding	denotes	binding
T8531	7499-7502	Protein	denotes	p65
T23896	1071-1074	Protein	denotes	Tat
T23897	1203-1212	Protein	denotes	siRNA Tat
T23898	1351-1354	Protein	denotes	p24
T23899	1397-1400	Protein	denotes	Tat
T23900	1401-1411	Gene_expression	denotes	expression		true
T23901	1466-1476	Protein	denotes	luciferase
T23902	1574-1577	Protein	denotes	p65
T23903	1582-1585	Protein	denotes	p50
T23904	1586-1593	Binding	denotes	binding		true
T23905	1586-1593	Binding	denotes	binding		true
T23906	1710-1720	Gene_expression	denotes	expression		true
T23907	1710-1720	Gene_expression	denotes	expression		true
T23908	1724-1727	Protein	denotes	p65
T23909	1732-1737	Protein	denotes	IκB-α
T23910	1886-1899	Protein	denotes	Hexokinase II
T8412	0-3	Protein	denotes	Tat
T8413	35-44	Binding	denotes	hijacking
T8414	45-50	Protein	denotes	IκB-α
T8415	183-193	Regulation	denotes	modulating
T8416	198-208	Gene_expression	denotes	expression
T8417	212-215	Protein	denotes	Tat
T8418	274-283	Protein	denotes	siRNA Tat
T8419	368-371	Protein	denotes	Env
T8420	547-557	Gene_expression	denotes	expression
T8421	561-564	Protein	denotes	Tat
T8460	3195-3203	Positive_regulation	denotes	observed
T8461	3501-3506	Protein	denotes	IκB-α
T8462	3507-3518	Protein_catabolism	denotes	degradation
T8463	3552-3555	Protein	denotes	Tat
T8464	3597-3600	Protein	denotes	Tat
T8465	3649-3658	Gene_expression	denotes	synthesis
T8466	3662-3667	Protein	denotes	IκB-α
T8467	3706-3709	Protein	denotes	Tat
T8468	3814-3817	Protein	denotes	Tat
T8469	3980-3983	Protein	denotes	p65
T8470	3988-3995	Binding	denotes	binding
T8471	4020-4023	Protein	denotes	p65
T8472	4059-4067	Positive_regulation	denotes	enhanced
T8473	4059-4067	Positive_regulation	denotes	enhanced
T8474	4071-4074	Protein	denotes	Tat
T8475	4086-4091	Protein	denotes	IκB-α
T8476	4122-4130	Regulation	denotes	affected	true
T8477	4184-4193	Positive_regulation	denotes	increased
T8478	4237-4240	Protein	denotes	p65
T8479	4272-4285	Regulation	denotes	independently	true
T8480	4305-4308	Protein	denotes	Tat
T8481	4347-4350	Protein	denotes	p65
T8482	4355-4362	Binding	denotes	binding
T8483	4375-4378	Protein	denotes	p65
T8484	4389-4397	Positive_regulation	denotes	elevated
T24339	5216-5219	Protein	denotes	Tat
T24340	5277-5288	Binding	denotes	associating
T24341	5294-5299	Protein	denotes	IκB-α
T24342	5391-5394	Protein	denotes	Tat
T24343	5678-5681	Protein	denotes	p65
T24344	5683-5686	Protein	denotes	p50
T24345	5691-5699	Protein	denotes	FLAG-Tat
T24346	5700-5707	Binding	denotes	binding		true
T24347	5700-5707	Binding	denotes	binding		true
T24348	5700-5707	Binding	denotes	binding		true
T24349	6303-6306	Protein	denotes	Tat
T24350	6507-6516	Protein	denotes	protein G
R6981	T8444	T8442	causeOf	Tat,elevated
R6997	T8474	T8473	causeOf	Tat,enhanced
R6998	T8474	T8476	causeOf	Tat,affected
R6999	T8475	T8476	themeOf	IκB-α,affected
R7000	T8477	T8479	themeOf	increased,independently
R7001	T8478	T8477	themeOf	p65,increased
R7002	T8480	T8479	causeOf	Tat,independently
R7003	T8481	T8482	themeOf	p65,binding
R7004	T8482	T8484	themeOf	binding,elevated
R7005	T8482	T8487	themeOf	binding,dropped
R7006	T8483	T8485	themeOf	p65,elevated
R7007	T8483	T8488	themeOf	p65,dropped
R7008	T8486	T8484	causeOf	Tat,elevated
R7009	T8486	T8485	causeOf	Tat,elevated
R7010	T8489	T8490	themeOf	Tat,negative
R7011	T8490	T8487	causeOf	negative,dropped
R7012	T8490	T8488	causeOf	negative,dropped
R7013	T8491	T8492	themeOf	Tat,bound
R7014	T8491	T8493	themeOf	Tat,binding
R7015	T8493	T8494	themeOf	binding,increased
R7016	T8495	T8497	themeOf	Degradation,induced
R7017	T8496	T8495	themeOf	IκB-α,Degradation
R7018	T8496	T8499	themeOf	IκB-α,synthesis
R7019	T8497	T8498	causeOf	induced,followed
R7020	T8499	T8498	themeOf	synthesis,followed
R7021	T8502	T8503	causeOf	Tat,affecting
R7022	T8504	T8503	themeOf	IκB-α,affecting
R7023	T8505	T8506	themeOf	Tat,binds
R7024	T8507	T8506	themeOf	sixth ankyrin,binds
R7025	T8507	T8508	partOf	sixth ankyrin,IκB-α
R7026	T8509	T8510	causeOf	Tat,counteracted
R7027	T8511	T8510	themeOf	generation,counteracted
R7028	T8512	T8511	themeOf	IκB-α,generation
R7029	T8514	T8513	themeOf	IκB-α,association
R7030	T8515	T8513	themeOf	p65,association
R7031	T8517	T8516	themeOf	IκB-α,synthesis
R7032	T8518	T8522	themeOf	Coimmunoprecipitation,halved
R7033	T8519	T8518	themeOf	IκB-α,Coimmunoprecipitation
R7034	T8520	T8518	themeOf	p65,Coimmunoprecipitation
R7035	T8523	T8522	causeOf	Tat,halved
R7036	T8524	T8525	themeOf	Tat,coimmunoprecipitated
R7037	T8526	T8525	themeOf	IκB-α,coimmunoprecipitated
R7038	T8527	T8528	causeOf	Tat,competed
R7039	T8529	T8530	themeOf	IκB-α,binding
R7040	T8530	T8528	themeOf	binding,competed
R7041	T8531	T8530	themeOf	p65,binding
R19062	T23899	T23900	themeOf	Tat,expression
R19063	T23902	T23904	themeOf	p65,binding
R19064	T23903	T23905	themeOf	p50,binding
R19065	T23908	T23906	themeOf	p65,expression
R19066	T23909	T23907	themeOf	IκB-α,expression
R6963	T8414	T8413	themeOf	IκB-α,hijacking
R6964	T8416	T8415	themeOf	expression,modulating
R6965	T8417	T8416	themeOf	Tat,expression
R6966	T8420	T8422	themeOf	expression,increased
R6967	T8420	T8423	themeOf	expression,barely detected
R6968	T8421	T8420	themeOf	Tat,expression
R6969	T8424	T8423	causeOf	siRNA Tat,barely detected
R6970	T8425	T8428	themeOf	dependent,induced
R6971	T8426	T8427	themeOf	luciferase,expression
R6972	T8427	T8425	themeOf	expression,dependent
R6973	T8431	T8434	themeOf	p65,binding
R6974	T8432	T8433	themeOf	p50,binding
R6975	T8439	T8440	themeOf	p65,binding
R6976	T8440	T8443	themeOf	binding,elevated
R6978	T8441	T8442	themeOf	p65,elevated
R6980	T8444	T8443	causeOf	Tat,elevated
R6962	T8412	T8413	themeOf	Tat,hijacking
R6977	T8440	T8445	themeOf	binding,dropped
R6979	T8441	T8446	themeOf	p65,dropped
R6982	T8447	T8445	causeOf	absence,dropped
R6983	T8447	T8446	causeOf	absence,dropped
R6984	T8448	T8447	themeOf	Tat,absence
R6985	T8450	T8451	themeOf	IκB-α,degraded
R6986	T8450	T8452	themeOf	IκB-α,synthesized
R6987	T8456	T8457	themeOf	IκB-α,degradation
R6988	T8456	T8458	themeOf	IκB-α,re-synthesis
R6989	T8457	T8460	themeOf	degradation,observed
R6990	T8458	T8459	themeOf	re-synthesis,observed
R6991	T8461	T8462	themeOf	IκB-α,degradation
R6992	T8466	T8465	themeOf	IκB-α,synthesis
R6993	T8469	T8470	themeOf	p65,binding
R6994	T8470	T8472	themeOf	binding,enhanced
R6995	T8471	T8473	themeOf	p65,enhanced
R6996	T8474	T8472	causeOf	Tat,enhanced
R19349	T24339	T24340	themeOf	Tat,associating
R19350	T24341	T24340	themeOf	IκB-α,associating
R19351	T24343	T24347	themeOf	p65,binding
R19352	T24344	T24346	themeOf	p50,binding
R19353	T24345	T24348	themeOf	FLAG-Tat,binding

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T9369	0-3	Q8UMQ1	denotes	Tat
T9370	0-3	P04610	denotes	Tat
T9371	0-3	P04608	denotes	Tat
T9372	0-3	P04605	denotes	Tat
T9373	212-215	Q8UMQ1	denotes	Tat
T9374	212-215	P04608	denotes	Tat
T9375	212-215	P04610	denotes	Tat
T9376	212-215	P04605	denotes	Tat
T9377	280-283	P04610	denotes	Tat
T9378	280-283	P04608	denotes	Tat
T9379	280-283	P04605	denotes	Tat
T9380	280-283	Q8UMQ1	denotes	Tat
T9381	368-371	P04578	denotes	Env
T9382	561-564	P04608	denotes	Tat
T9383	561-564	P04610	denotes	Tat
T9384	561-564	P04605	denotes	Tat
T9385	561-564	Q8UMQ1	denotes	Tat
T9386	731-734	P04608	denotes	Tat
T9387	731-734	P04605	denotes	Tat
T9388	731-734	P04610	denotes	Tat
T9389	731-734	Q8UMQ1	denotes	Tat
T9390	784-794	P08659	denotes	luciferase
T9391	958-961	P04608	denotes	Tat
T9392	958-961	P04610	denotes	Tat
T9393	958-961	P04605	denotes	Tat
T9394	958-961	Q8UMQ1	denotes	Tat
T9395	1001-1004	P04605	denotes	Tat
T9396	1001-1004	P04608	denotes	Tat
T9397	1001-1004	P04610	denotes	Tat
T9398	1001-1004	Q8UMQ1	denotes	Tat
T9399	2313-2316	P21579	denotes	p65
T9400	2313-2316	Q04206	denotes	p65
T9401	2317-2320	P19838	denotes	p50
T9402	2391-2394	Q04206	denotes	p65
T9403	2391-2394	P21579	denotes	p65
T9404	2466-2469	P04608	denotes	Tat
T9405	2466-2469	P04610	denotes	Tat
T9406	2466-2469	Q8UMQ1	denotes	Tat
T9407	2466-2469	P04605	denotes	Tat
T9408	2511-2514	P04610	denotes	Tat
T9409	2511-2514	P04605	denotes	Tat
T9410	2511-2514	P04608	denotes	Tat
T9411	2511-2514	Q8UMQ1	denotes	Tat
T9412	2531-2534	P04608	denotes	Tat
T9413	2531-2534	P04610	denotes	Tat
T9414	2531-2534	Q8UMQ1	denotes	Tat
T9415	2531-2534	P04605	denotes	Tat
T9416	2580-2583	Q04206	denotes	p65
T9417	2580-2583	P21579	denotes	p65
T9418	2608-2611	P21579	denotes	p65
T9419	2608-2611	Q04206	denotes	p65
T9420	2646-2649	P04605	denotes	Tat
T9421	2646-2649	Q8UMQ1	denotes	Tat
T9422	2646-2649	P04610	denotes	Tat
T9423	2646-2649	P04608	denotes	Tat
T9424	2713-2716	P04610	denotes	Tat
T9425	2713-2716	P04608	denotes	Tat
T9426	2713-2716	P04605	denotes	Tat
T9427	2713-2716	Q8UMQ1	denotes	Tat
T9428	2724-2727	P04610	denotes	Tat
T9429	2724-2727	Q8UMQ1	denotes	Tat
T9430	2724-2727	P04608	denotes	Tat
T9431	2724-2727	P04605	denotes	Tat
T9432	2894-2897	Q8UMQ1	denotes	Tat
T9433	2894-2897	P04605	denotes	Tat
T9434	2894-2897	P04608	denotes	Tat
T9435	2894-2897	P04610	denotes	Tat
T9436	2911-2914	Q8UMQ1	denotes	Tat
T9437	2911-2914	P04610	denotes	Tat
T9438	2911-2914	P04608	denotes	Tat
T9439	2911-2914	P04605	denotes	Tat
T9440	3087-3090	Q8UMQ1	denotes	Tat
T9441	3087-3090	P04610	denotes	Tat
T9442	3087-3090	P04608	denotes	Tat
T9443	3087-3090	P04605	denotes	Tat
T9444	3552-3555	Q8UMQ1	denotes	Tat
T9445	3552-3555	P04610	denotes	Tat
T9446	3552-3555	P04608	denotes	Tat
T9447	3552-3555	P04605	denotes	Tat
T9448	3597-3600	P04610	denotes	Tat
T9449	3597-3600	P04608	denotes	Tat
T9450	3597-3600	P04605	denotes	Tat
T9451	3597-3600	Q8UMQ1	denotes	Tat
T9452	3706-3709	P04605	denotes	Tat
T9453	3706-3709	Q8UMQ1	denotes	Tat
T9454	3706-3709	P04610	denotes	Tat
T9455	3706-3709	P04608	denotes	Tat
T9456	3814-3817	P04608	denotes	Tat
T9457	3814-3817	Q8UMQ1	denotes	Tat
T9458	3814-3817	P04610	denotes	Tat
T9459	3814-3817	P04605	denotes	Tat
T9460	3980-3983	P21579	denotes	p65
T9461	3980-3983	Q04206	denotes	p65
T9462	4020-4023	Q04206	denotes	p65
T9463	4020-4023	P21579	denotes	p65
T9464	4071-4074	P04608	denotes	Tat
T9465	4071-4074	P04610	denotes	Tat
T9466	4071-4074	Q8UMQ1	denotes	Tat
T9467	4071-4074	P04605	denotes	Tat
T9468	4237-4240	P21579	denotes	p65
T9469	4237-4240	Q04206	denotes	p65
T9470	4305-4308	P04608	denotes	Tat
T9471	4305-4308	P04610	denotes	Tat
T9472	4305-4308	Q8UMQ1	denotes	Tat
T9473	4305-4308	P04605	denotes	Tat
T9474	4347-4350	Q04206	denotes	p65
T9475	4347-4350	P21579	denotes	p65
T9476	4375-4378	Q04206	denotes	p65
T9477	4375-4378	P21579	denotes	p65
T9478	4427-4430	P04610	denotes	Tat
T9479	4427-4430	Q8UMQ1	denotes	Tat
T9480	4427-4430	P04608	denotes	Tat
T9481	4427-4430	P04605	denotes	Tat
T9482	4463-4466	P04610	denotes	Tat
T9483	4463-4466	P04605	denotes	Tat
T9484	4463-4466	P04608	denotes	Tat
T9485	4463-4466	Q8UMQ1	denotes	Tat
T9486	4514-4517	P04610	denotes	Tat
T9487	4514-4517	P04608	denotes	Tat
T9488	4514-4517	P04605	denotes	Tat
T9489	4514-4517	Q8UMQ1	denotes	Tat
T9490	4869-4872	P04608	denotes	Tat
T9491	4869-4872	P04605	denotes	Tat
T9492	4869-4872	P04610	denotes	Tat
T9493	4869-4872	Q8UMQ1	denotes	Tat
T9494	5032-5035	P04605	denotes	Tat
T9495	5032-5035	P04608	denotes	Tat
T9496	5032-5035	Q8UMQ1	denotes	Tat
T9497	5032-5035	P04610	denotes	Tat
T9498	5078-5081	P04605	denotes	Tat
T9499	5078-5081	P04608	denotes	Tat
T9500	5078-5081	P04610	denotes	Tat
T9501	5078-5081	Q8UMQ1	denotes	Tat
T9502	6850-6853	P04610	denotes	Tat
T9503	6850-6853	P04608	denotes	Tat
T9504	6850-6853	Q8UMQ1	denotes	Tat
T9505	6850-6853	P04605	denotes	Tat
T9506	6917-6920	Q8UMQ1	denotes	Tat
T9507	6917-6920	P04605	denotes	Tat
T9508	6917-6920	P04608	denotes	Tat
T9509	6917-6920	P04610	denotes	Tat
T9510	7020-7023	P21579	denotes	p65
T9511	7020-7023	Q04206	denotes	p65
T9512	7241-7244	Q04206	denotes	p65
T9513	7241-7244	P21579	denotes	p65
T9514	7294-7297	P04605	denotes	Tat
T9515	7294-7297	P04608	denotes	Tat
T9516	7294-7297	Q8UMQ1	denotes	Tat
T9517	7294-7297	P04610	denotes	Tat
T9518	7359-7362	P04610	denotes	Tat
T9519	7359-7362	P04608	denotes	Tat
T9520	7359-7362	Q8UMQ1	denotes	Tat
T9521	7359-7362	P04605	denotes	Tat
T9522	7385-7388	P04605	denotes	Tat
T9523	7385-7388	P04608	denotes	Tat
T9524	7385-7388	Q8UMQ1	denotes	Tat
T9525	7385-7388	P04610	denotes	Tat
T9526	7465-7468	P04608	denotes	Tat
T9527	7465-7468	Q8UMQ1	denotes	Tat
T9528	7465-7468	P04610	denotes	Tat
T9529	7465-7468	P04605	denotes	Tat
T9530	7499-7502	Q04206	denotes	p65
T9531	7499-7502	P21579	denotes	p65
T23916	1071-1074	P04605	denotes	Tat
T23917	1071-1074	P04610	denotes	Tat
T23918	1071-1074	P04608	denotes	Tat
T23919	1071-1074	Q8UMQ1	denotes	Tat
T23920	1209-1212	P04605	denotes	Tat
T23921	1209-1212	P04610	denotes	Tat
T23922	1209-1212	Q8UMQ1	denotes	Tat
T23923	1209-1212	P04608	denotes	Tat
T23924	1351-1354	P04591	denotes	p24
T23925	1351-1354	P03366	denotes	p24
T23926	1351-1354	P12497	denotes	p24
T23927	1351-1354	P04585	denotes	p24
T23928	1397-1400	P04608	denotes	Tat
T23929	1397-1400	Q8UMQ1	denotes	Tat
T23930	1397-1400	P04610	denotes	Tat
T23931	1397-1400	P04605	denotes	Tat
T23932	1466-1476	P08659	denotes	luciferase
T23933	1574-1577	Q04206	denotes	p65
T23934	1574-1577	P21579	denotes	p65
T23935	1582-1585	P19838	denotes	p50
T23936	1724-1727	P21579	denotes	p65
T23937	1724-1727	Q04206	denotes	p65
T23938	1840-1843	Q04206	denotes	p65
T23939	1840-1843	P21579	denotes	p65
T24641	5216-5219	Q8UMQ1	denotes	Tat
T24642	5216-5219	P04610	denotes	Tat
T24643	5216-5219	P04608	denotes	Tat
T24644	5216-5219	P04605	denotes	Tat
T24645	5391-5394	P04605	denotes	Tat
T24646	5391-5394	P04610	denotes	Tat
T24647	5391-5394	P04608	denotes	Tat
T24648	5391-5394	Q8UMQ1	denotes	Tat
T24649	5678-5681	Q04206	denotes	p65
T24650	5678-5681	P21579	denotes	p65
T24651	5683-5686	P19838	denotes	p50
T24652	5696-5699	P04608	denotes	Tat
T24653	5696-5699	P04605	denotes	Tat
T24654	5696-5699	P04610	denotes	Tat
T24655	5696-5699	Q8UMQ1	denotes	Tat
T24656	5938-5941	P21579	denotes	p65
T24657	5938-5941	Q04206	denotes	p65
T24658	6303-6306	P04608	denotes	Tat
T24659	6303-6306	Q8UMQ1	denotes	Tat
T24660	6303-6306	P04610	denotes	Tat
T24661	6303-6306	P04605	denotes	Tat
T24662	6646-6649	Q04206	denotes	p65
T24663	6646-6649	P21579	denotes	p65

PMC:3333881 / 14991-22494 JSONTXT

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PMC:3333881 / 14991-22494 JSON TXT