PMC:3320587 / 43993-45076 JSONTXT

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adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T14926","span":{"begin":847,"end":852},"obj":"Protein"},{"id":"T14925","span":{"begin":775,"end":778},"obj":"Protein"},{"id":"T14924","span":{"begin":702,"end":707},"obj":"Protein"},{"id":"T14923","span":{"begin":693,"end":696},"obj":"Protein"},{"id":"T14922","span":{"begin":654,"end":659},"obj":"Protein"},{"id":"T14921","span":{"begin":584,"end":589},"obj":"Protein"},{"id":"T14920","span":{"begin":550,"end":559},"obj":"Protein"},{"id":"T14919","span":{"begin":386,"end":391},"obj":"Protein"},{"id":"T14918","span":{"begin":338,"end":343},"obj":"Protein"},{"id":"T14917","span":{"begin":306,"end":327},"obj":"Protein"},{"id":"T14916","span":{"begin":195,"end":200},"obj":"Protein"},{"id":"T14915","span":{"begin":50,"end":54},"obj":"Protein"},{"id":"T14914","span":{"begin":21,"end":26},"obj":"Protein"},{"id":"T14933","span":{"begin":1077,"end":1082},"obj":"Protein"},{"id":"T14932","span":{"begin":1053,"end":1058},"obj":"Protein"},{"id":"T14931","span":{"begin":1011,"end":1015},"obj":"Protein"},{"id":"T14930","span":{"begin":974,"end":977},"obj":"Protein"},{"id":"T14929","span":{"begin":964,"end":969},"obj":"Protein"},{"id":"T14928","span":{"begin":923,"end":928},"obj":"Protein"},{"id":"T14927","span":{"begin":870,"end":874},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T15614","span":{"begin":1053,"end":1058},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T15613","span":{"begin":964,"end":969},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T15612","span":{"begin":654,"end":659},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T15611","span":{"begin":195,"end":200},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T15642","span":{"begin":1011,"end":1015},"obj":"http://www.uniprot.org/uniprot/P42081"},{"id":"T15641","span":{"begin":870,"end":874},"obj":"http://www.uniprot.org/uniprot/P42081"},{"id":"T15638","span":{"begin":974,"end":977},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T15637","span":{"begin":775,"end":778},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T15636","span":{"begin":693,"end":696},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T15635","span":{"begin":550,"end":553},"obj":"http://www.uniprot.org/uniprot/Q9U6Y4"},{"id":"T15634","span":{"begin":50,"end":54},"obj":"http://www.uniprot.org/uniprot/O60603"},{"id":"T15626","span":{"begin":1077,"end":1082},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15625","span":{"begin":923,"end":928},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15624","span":{"begin":847,"end":852},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15623","span":{"begin":702,"end":707},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15622","span":{"begin":584,"end":589},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15621","span":{"begin":386,"end":391},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15620","span":{"begin":338,"end":343},"obj":"http://www.uniprot.org/uniprot/Q99836"},{"id":"T15619","span":{"begin":21,"end":26},"obj":"http://www.uniprot.org/uniprot/Q99836"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"GO-BP","denotations":[{"id":"T14973","span":{"begin":1039,"end":1049},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T14962","span":{"begin":201,"end":216},"obj":"http://purl.obolibrary.org/obo/GO_0010467"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"GO-CC","denotations":[{"id":"T14981","span":{"begin":606,"end":611},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14980","span":{"begin":496,"end":501},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14979","span":{"begin":413,"end":418},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"sentences","denotations":[{"id":"T14779","span":{"begin":1029,"end":1083},"obj":"Sentence"},{"id":"T14778","span":{"begin":887,"end":1028},"obj":"Sentence"},{"id":"T14777","span":{"begin":792,"end":886},"obj":"Sentence"},{"id":"T14776","span":{"begin":687,"end":791},"obj":"Sentence"},{"id":"T14775","span":{"begin":578,"end":686},"obj":"Sentence"},{"id":"T14774","span":{"begin":345,"end":577},"obj":"Sentence"},{"id":"T14773","span":{"begin":84,"end":344},"obj":"Sentence"},{"id":"T14772","span":{"begin":0,"end":83},"obj":"Sentence"},{"id":"T259","span":{"begin":0,"end":83},"obj":"Sentence"},{"id":"T260","span":{"begin":84,"end":344},"obj":"Sentence"},{"id":"T261","span":{"begin":345,"end":577},"obj":"Sentence"},{"id":"T262","span":{"begin":578,"end":686},"obj":"Sentence"},{"id":"T263","span":{"begin":687,"end":791},"obj":"Sentence"},{"id":"T264","span":{"begin":792,"end":886},"obj":"Sentence"},{"id":"T265","span":{"begin":887,"end":1028},"obj":"Sentence"},{"id":"T266","span":{"begin":1029,"end":1083},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

{"project":"simple1","denotations":[{"id":"T15013","span":{"begin":1077,"end":1082},"obj":"Protein"},{"id":"T15012","span":{"begin":1053,"end":1058},"obj":"Protein"},{"id":"T15011","span":{"begin":1011,"end":1015},"obj":"Protein"},{"id":"T15010","span":{"begin":974,"end":977},"obj":"Protein"},{"id":"T15009","span":{"begin":964,"end":969},"obj":"Protein"},{"id":"T15008","span":{"begin":923,"end":928},"obj":"Protein"},{"id":"T15007","span":{"begin":870,"end":874},"obj":"Protein"},{"id":"T15006","span":{"begin":847,"end":852},"obj":"Protein"},{"id":"T15005","span":{"begin":775,"end":778},"obj":"Protein"},{"id":"T15004","span":{"begin":702,"end":707},"obj":"Protein"},{"id":"T15003","span":{"begin":693,"end":696},"obj":"Protein"},{"id":"T15002","span":{"begin":654,"end":659},"obj":"Protein"},{"id":"T15001","span":{"begin":584,"end":589},"obj":"Protein"},{"id":"T15000","span":{"begin":550,"end":559},"obj":"Protein"},{"id":"T14999","span":{"begin":386,"end":391},"obj":"Protein"},{"id":"T14998","span":{"begin":338,"end":343},"obj":"Protein"},{"id":"T14997","span":{"begin":306,"end":327},"obj":"Protein"},{"id":"T14996","span":{"begin":195,"end":200},"obj":"Protein"},{"id":"T14995","span":{"begin":50,"end":54},"obj":"Protein"},{"id":"T14994","span":{"begin":21,"end":26},"obj":"Protein"}],"text":"The adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

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To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. 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adaptor molecule MyD88 transmits signals upon TLR2 ligation (reviewed in [53]). To confirm that this protein and, therefore, that this TLR-dependent pathway is important for MTb induction of NFAT5 gene expression, we constructed a THP-1 cell line that constitutively expresses a lentivirally-delivered short hairpin (sh)RNA targeting MyD88. Real-time PCR analysis demonstrated that MyD88 mRNA levels in these cells were constitutively and significantly reduced in comparison to control THP-1 cells transduced with a lentivirus expressing control GFP shRNA (data not shown). These MyD88 knockdown THP-1 cells were then infected with MTb (CDC1551) and NFAT5 mRNA levels were examined. Since TNF is a MyD88-dependent gene [21], [54], [55], as a positive control we measured TNF mRNA levels. For a negative control, we measured mRNA levels of the MyD88-independent gene CD86 [55], [56]. As shown in Figure 7B, knockdown of MyD88 specifically inhibited MTb-induced NFAT5 and TNF mRNA levels and had no impact on CD86 mRNA levels. Thus, MTb regulation of NFAT5 is dependent upon MyD88."}

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