PMC:3320587 / 25399-26031 JSONTXT

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we knocked down NFAT5 mRNA levels in MDM using an siRNA that suppresses both NFAT5 mRNA and NFAT5 protein levels [31]. As shown in Figure 2, transfection of the siRNA specific for NFAT5 reduces NFAT5 mRNA levels in both MTb-uninfected (p = 0.048) and MTb-infected (p = 0.021) MDM as compared to transfection of control GFP siRNA into MTb-uninfected or -infected MDM (Figure 2B). We note that although siRNA normally is effective for 48–72 hours in cell lines that divide rapidly, in human MDM, which are non-dividing cells, siRNA to host factors remains detectable and functional up to at least 15 days post-transfection [47]."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T8914","span":{"begin":325,"end":328},"obj":"Protein"},{"id":"T8913","span":{"begin":200,"end":205},"obj":"Protein"},{"id":"T8912","span":{"begin":186,"end":191},"obj":"Protein"},{"id":"T8911","span":{"begin":98,"end":103},"obj":"Protein"},{"id":"T8910","span":{"begin":83,"end":88},"obj":"Protein"},{"id":"T8909","span":{"begin":22,"end":27},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Next, we knocked down NFAT5 mRNA levels in MDM using an siRNA that suppresses both NFAT5 mRNA and NFAT5 protein levels [31]. As shown in Figure 2, transfection of the siRNA specific for NFAT5 reduces NFAT5 mRNA levels in both MTb-uninfected (p = 0.048) and MTb-infected (p = 0.021) MDM as compared to transfection of control GFP siRNA into MTb-uninfected or -infected MDM (Figure 2B). We note that although siRNA normally is effective for 48–72 hours in cell lines that divide rapidly, in human MDM, which are non-dividing cells, siRNA to host factors remains detectable and functional up to at least 15 days post-transfection [47]."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T9465","span":{"begin":200,"end":205},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T9464","span":{"begin":186,"end":191},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T9463","span":{"begin":98,"end":103},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T9462","span":{"begin":83,"end":88},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T9461","span":{"begin":22,"end":27},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T9475","span":{"begin":325,"end":328},"obj":"http://www.uniprot.org/uniprot/Q9U6Y4"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Next, we knocked down NFAT5 mRNA levels in MDM using an siRNA that suppresses both NFAT5 mRNA and NFAT5 protein levels [31]. As shown in Figure 2, transfection of the siRNA specific for NFAT5 reduces NFAT5 mRNA levels in both MTb-uninfected (p = 0.048) and MTb-infected (p = 0.021) MDM as compared to transfection of control GFP siRNA into MTb-uninfected or -infected MDM (Figure 2B). We note that although siRNA normally is effective for 48–72 hours in cell lines that divide rapidly, in human MDM, which are non-dividing cells, siRNA to host factors remains detectable and functional up to at least 15 days post-transfection [47]."}

{"project":"GO-CC","denotations":[{"id":"T8938","span":{"begin":523,"end":528},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T8937","span":{"begin":454,"end":458},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T8942","span":{"begin":539,"end":543},"obj":"http://purl.obolibrary.org/obo/GO_0018995"}],"text":"Next, we knocked down NFAT5 mRNA levels in MDM using an siRNA that suppresses both NFAT5 mRNA and NFAT5 protein levels [31]. As shown in Figure 2, transfection of the siRNA specific for NFAT5 reduces NFAT5 mRNA levels in both MTb-uninfected (p = 0.048) and MTb-infected (p = 0.021) MDM as compared to transfection of control GFP siRNA into MTb-uninfected or -infected MDM (Figure 2B). We note that although siRNA normally is effective for 48–72 hours in cell lines that divide rapidly, in human MDM, which are non-dividing cells, siRNA to host factors remains detectable and functional up to at least 15 days post-transfection [47]."}

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{"project":"simple1","denotations":[{"id":"T8981","span":{"begin":325,"end":328},"obj":"Protein"},{"id":"T8980","span":{"begin":200,"end":205},"obj":"Protein"},{"id":"T8979","span":{"begin":186,"end":191},"obj":"Protein"},{"id":"T8978","span":{"begin":98,"end":103},"obj":"Protein"},{"id":"T8977","span":{"begin":83,"end":88},"obj":"Protein"},{"id":"T8976","span":{"begin":22,"end":27},"obj":"Protein"}],"text":"Next, we knocked down NFAT5 mRNA levels in MDM using an siRNA that suppresses both NFAT5 mRNA and NFAT5 protein levels [31]. As shown in Figure 2, transfection of the siRNA specific for NFAT5 reduces NFAT5 mRNA levels in both MTb-uninfected (p = 0.048) and MTb-infected (p = 0.021) MDM as compared to transfection of control GFP siRNA into MTb-uninfected or -infected MDM (Figure 2B). We note that although siRNA normally is effective for 48–72 hours in cell lines that divide rapidly, in human MDM, which are non-dividing cells, siRNA to host factors remains detectable and functional up to at least 15 days post-transfection [47]."}

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