PMC:3320587 / 14767-15102 JSONTXT

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    pmc-enju-pas

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mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    bionlp-st-ge-2016-test-proteins

    {"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T6534","span":{"begin":266,"end":273},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6593","span":{"begin":266,"end":273},"obj":"http://www.uniprot.org/uniprot/P60709"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    sentences

    {"project":"sentences","denotations":[{"id":"T6532","span":{"begin":232,"end":335},"obj":"Sentence"},{"id":"T6531","span":{"begin":126,"end":231},"obj":"Sentence"},{"id":"T6530","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"T92","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"T93","span":{"begin":126,"end":231},"obj":"Sentence"},{"id":"T94","span":{"begin":232,"end":335},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    simple1

    {"project":"simple1","denotations":[{"id":"T6535","span":{"begin":266,"end":273},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    BioNLP16_DUT

    {"project":"BioNLP16_DUT","denotations":[{"id":"T6667","span":{"begin":266,"end":273},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    BioNLP16_Messiy

    {"project":"BioNLP16_Messiy","denotations":[{"id":"T6597","span":{"begin":266,"end":273},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    DLUT931

    {"project":"DLUT931","denotations":[{"id":"T6596","span":{"begin":274,"end":278},"obj":"Transcription"},{"id":"T6595","span":{"begin":266,"end":273},"obj":"Protein"}],"relations":[{"id":"R5288","pred":"themeOf","subj":"T6595","obj":"T6596"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    bionlp-st-ge-2016-test-ihmc

    {"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T6674","span":{"begin":0,"end":26},"obj":"Transcription"},{"id":"T6673","span":{"begin":0,"end":26},"obj":"Gene_expression"},{"id":"T6672","span":{"begin":4,"end":8},"obj":"Protein"},{"id":"T6670","span":{"begin":43,"end":124},"obj":"Protein"},{"id":"T6669","span":{"begin":266,"end":278},"obj":"Protein"},{"id":"T6668","span":{"begin":266,"end":278},"obj":"Protein"}],"relations":[{"id":"R5355","pred":"themeOf","subj":"T6672","obj":"T6673"},{"id":"R5356","pred":"themeOf","subj":"T6672","obj":"T6674"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    bionlp-st-ge-2016-spacy-parsed

    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mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    bionlp-st-ge-2016-test-tees

    {"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T6599","span":{"begin":266,"end":278},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    testone

    {"project":"testone","denotations":[{"id":"T6525","span":{"begin":4,"end":19},"obj":"Transcription"},{"id":"T6524","span":{"begin":266,"end":273},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}

    test3

    {"project":"test3","denotations":[{"id":"T6528","span":{"begin":266,"end":273},"obj":"Protein"},{"id":"T6527","span":{"begin":4,"end":19},"obj":"Transcription"},{"id":"T6526","span":{"begin":266,"end":273},"obj":"Protein"}],"text":"The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com). The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min. The results were normalized using β-actin mRNA as an internal control and expressed as relative values."}