PMC:3317373 / 7878-8254 JSONTXT

{"project":"pmc-enju-pas","denotations":[{"id":"T3477","span":{"begin":374,"end":375},"obj":"-RRB-"},{"id":"T3476","span":{"begin":371,"end":374},"obj":"NNP"},{"id":"T3475","span":{"begin":369,"end":370},"obj":"-COMMA-"},{"id":"T3474","span":{"begin":367,"end":369},"obj":"NNP"},{"id":"T3473","span":{"begin":365,"end":366},"obj":"-COMMA-"},{"id":"T3472","span":{"begin":359,"end":365},"obj":"NNP"},{"id":"T3471","span":{"begin":358,"end":359},"obj":"-LRB-"},{"id":"T3470","span":{"begin":350,"end":357},"obj":"NNP"},{"id":"T3469","span":{"begin":344,"end":349},"obj":"NNP"},{"id":"T3468","span":{"begin":336,"end":343},"obj":"NNP"},{"id":"T3467","span":{"begin":331,"end":335},"obj":"NNP"},{"id":"T3466","span":{"begin":321,"end":330},"obj":"NNP"},{"id":"T3465","span":{"begin":318,"end":320},"obj":"IN"},{"id":"T3464","span":{"begin":308,"end":317},"obj":"VB"},{"id":"T3463","span":{"begin":303,"end":307},"obj":"VB"},{"id":"T3462","span":{"begin":288,"end":302},"obj":"NN"},{"id":"T3461","span":{"begin":280,"end":287},"obj":"NN"},{"id":"T3460","span":{"begin":275,"end":278},"obj":"NN"},{"id":"T3459","span":{"begin":272,"end":274},"obj":"IN"},{"id":"T3458","span":{"begin":268,"end":271},"obj":"NN"},{"id":"T3457","span":{"begin":265,"end":267},"obj":"CD"},{"id":"T3456","span":{"begin":261,"end":264},"obj":"IN"},{"id":"T3455","span":{"begin":259,"end":260},"obj":"NN"},{"id":"T3454","span":{"begin":252,"end":258},"obj":"CD"},{"id":"T3453","span":{"begin":249,"end":251},"obj":"IN"},{"id":"T3452","span":{"begin":237,"end":248},"obj":"VB"},{"id":"T3451","span":{"begin":233,"end":236},"obj":"CC"},{"id":"T3450","span":{"begin":231,"end":232},"obj":"-RRB-"},{"id":"T3449","span":{"begin":228,"end":231},"obj":"CD"},{"id":"T3448","span":{"begin":225,"end":227},"obj":"NN"},{"id":"T3447","span":{"begin":221,"end":224},"obj":"NNP"},{"id":"T3446","span":{"begin":218,"end":220},"obj":"IN"},{"id":"T3445","span":{"begin":209,"end":217},"obj":"NN"},{"id":"T3444","span":{"begin":199,"end":208},"obj":"NN"},{"id":"T3443","span":{"begin":190,"end":198},"obj":"NN"},{"id":"T3442","span":{"begin":186,"end":189},"obj":"CC"},{"id":"T3441","span":{"begin":184,"end":185},"obj":"-COMMA-"},{"id":"T3440","span":{"begin":180,"end":184},"obj":"NN"},{"id":"T3439","span":{"begin":177,"end":179},"obj":"NN"},{"id":"T3438","span":{"begin":175,"end":176},"obj":"CD"},{"id":"T3437","span":{"begin":173,"end":174},"obj":"-COMMA-"},{"id":"T3436","span":{"begin":170,"end":173},"obj":"NN"},{"id":"T3435","span":{"begin":167,"end":169},"obj":"NN"},{"id":"T3434","span":{"begin":163,"end":166},"obj":"CD"},{"id":"T3433","span":{"begin":161,"end":162},"obj":"-COMMA-"},{"id":"T3432","span":{"begin":158,"end":161},"obj":"NN"},{"id":"T3431","span":{"begin":155,"end":157},"obj":"NN"},{"id":"T3430","span":{"begin":152,"end":154},"obj":"CD"},{"id":"T3429","span":{"begin":150,"end":151},"obj":"-COMMA-"},{"id":"T3428","span":{"begin":144,"end":150},"obj":"NN"},{"id":"T3427","span":{"begin":141,"end":143},"obj":"NN"},{"id":"T3426","span":{"begin":139,"end":140},"obj":"CD"},{"id":"T3425","span":{"begin":137,"end":138},"obj":"-COMMA-"},{"id":"T3424","span":{"begin":133,"end":137},"obj":"NN"},{"id":"T3423","span":{"begin":130,"end":132},"obj":"NN"},{"id":"T3422","span":{"begin":128,"end":129},"obj":"CD"},{"id":"T3421","span":{"begin":126,"end":127},"obj":"-COMMA-"},{"id":"T3420","span":{"begin":122,"end":126},"obj":"NN"},{"id":"T3419","span":{"begin":119,"end":121},"obj":"NN"},{"id":"T3418","span":{"begin":117,"end":118},"obj":"CD"},{"id":"T3417","span":{"begin":115,"end":116},"obj":"-COMMA-"},{"id":"T3416","span":{"begin":112,"end":115},"obj":"NN"},{"id":"T3415","span":{"begin":110,"end":111},"obj":"NN"},{"id":"T3414","span":{"begin":107,"end":110},"obj":"CD"},{"id":"T3413","span":{"begin":105,"end":106},"obj":"-COMMA-"},{"id":"T3412","span":{"begin":93,"end":105},"obj":"NN"},{"id":"T3411","span":{"begin":86,"end":92},"obj":"NN"},{"id":"T3410","span":{"begin":84,"end":85},"obj":"NN"},{"id":"T3409","span":{"begin":81,"end":84},"obj":"CD"},{"id":"T3408","span":{"begin":79,"end":80},"obj":"-COMMA-"},{"id":"T3407","span":{"begin":75,"end":79},"obj":"NN"},{"id":"T3406","span":{"begin":73,"end":74},"obj":"NN"},{"id":"T3405","span":{"begin":72,"end":73},"obj":"CD"},{"id":"T3404","span":{"begin":71,"end":72},"obj":"-LRB-"},{"id":"T3403","span":{"begin":64,"end":70},"obj":"NN"},{"id":"T3402","span":{"begin":59,"end":63},"obj":"NN"},{"id":"T3401","span":{"begin":56,"end":58},"obj":"IN"},{"id":"T3400","span":{"begin":44,"end":55},"obj":"VB"},{"id":"T3399","span":{"begin":39,"end":43},"obj":"VB"},{"id":"T3398","span":{"begin":33,"end":38},"obj":"NN"},{"id":"T3397","span":{"begin":31,"end":32},"obj":"-COMMA-"},{"id":"T3396","span":{"begin":24,"end":31},"obj":"NN"},{"id":"T3395","span":{"begin":16,"end":23},"obj":"NN"},{"id":"T3394","span":{"begin":10,"end":15},"obj":"JJ"},{"id":"T3393","span":{"begin":3,"end":9},"obj":"VB"},{"id":"T3392","span":{"begin":0,"end":2},"obj":"TO"}],"relations":[{"id":"R2900","pred":"arg1Of","subj":"T3393","obj":"T3392"},{"id":"R2901","pred":"arg2Of","subj":"T3396","obj":"T3393"},{"id":"R2902","pred":"arg1Of","subj":"T3396","obj":"T3394"},{"id":"R2903","pred":"arg1Of","subj":"T3396","obj":"T3395"},{"id":"R2904","pred":"arg1Of","subj":"T3398","obj":"T3393"},{"id":"R2905","pred":"arg1Of","subj":"T3398","obj":"T3399"},{"id":"R2906","pred":"arg2Of","subj":"T3398","obj":"T3400"},{"id":"R2907","pred":"arg2Of","subj":"T3398","obj":"T3452"},{"id":"R2908","pred":"arg1Of","subj":"T3400","obj":"T3401"},{"id":"R2909","pred":"arg1Of","subj":"T3400","obj":"T3451"},{"id":"R2910","pred":"arg2Of","subj":"T3403","obj":"T3401"},{"id":"R2911","pred":"arg1Of","subj":"T3403","obj":"T3402"},{"id":"R2912","pred":"arg1Of","subj":"T3403","obj":"T3404"},{"id":"R2913","pred":"arg1Of","subj":"T3405","obj":"T3406"},{"id":"R2914","pred":"arg1Of","subj":"T3407","obj":"T3405"},{"id":"R2915","pred":"arg1Of","subj":"T3407","obj":"T3408"},{"id":"R2916","pred":"arg1Of","subj":"T3408","obj":"T3413"},{"id":"R2917","pred":"arg1Of","subj":"T3409","obj":"T3410"},{"id":"R2918","pred":"arg2Of","subj":"T3412","obj":"T3408"},{"id":"R2919","pred":"arg1Of","subj":"T3412","obj":"T3409"},{"id":"R2920","pred":"arg1Of","subj":"T3412","obj":"T3411"},{"id":"R2921","pred":"arg1Of","subj":"T3413","obj":"T3417"},{"id":"R2922","pred":"arg1Of","subj":"T3414","obj":"T3415"},{"id":"R2923","pred":"arg2Of","subj":"T3416","obj":"T3413"},{"id":"R2924","pred":"arg1Of","subj":"T3416","obj":"T3414"},{"id":"R2925","pred":"arg1Of","subj":"T3417","obj":"T3421"},{"id":"R2926","pred":"arg1Of","subj":"T3418","obj":"T3419"},{"id":"R2927","pred":"arg2Of","subj":"T3420","obj":"T3417"},{"id":"R2928","pred":"arg1Of","subj":"T3420","obj":"T3418"},{"id":"R2929","pred":"arg2Of","subj":"T3421","obj":"T3404"},{"id":"R2930","pred":"arg1Of","subj":"T3421","obj":"T3425"},{"id":"R2931","pred":"arg1Of","subj":"T3422","obj":"T3423"},{"id":"R2932","pred":"arg2Of","subj":"T3424","obj":"T3421"},{"id":"R2933","pred":"arg1Of","subj":"T3424","obj":"T3422"},{"id":"R2934","pred":"arg1Of","subj":"T3426","obj":"T3427"},{"id":"R2935","pred":"arg1Of","subj":"T3428","obj":"T3426"},{"id":"R2936","pred":"arg1Of","subj":"T3428","obj":"T3429"},{"id":"R2937","pred":"arg1Of","subj":"T3429","obj":"T3433"},{"id":"R2938","pred":"arg1Of","subj":"T3430","obj":"T3431"},{"id":"R2939","pred":"arg2Of","subj":"T3432","obj":"T3429"},{"id":"R2940","pred":"arg1Of","subj":"T3432","obj":"T3430"},{"id":"R2941","pred":"arg1Of","subj":"T3433","obj":"T3437"},{"id":"R2942","pred":"arg1Of","subj":"T3434","obj":"T3435"},{"id":"R2943","pred":"arg2Of","subj":"T3436","obj":"T3433"},{"id":"R2944","pred":"arg1Of","subj":"T3436","obj":"T3434"},{"id":"R2945","pred":"arg1Of","subj":"T3437","obj":"T3442"},{"id":"R2946","pred":"arg1Of","subj":"T3438","obj":"T3439"},{"id":"R2947","pred":"arg2Of","subj":"T3440","obj":"T3437"},{"id":"R2948","pred":"arg1Of","subj":"T3440","obj":"T3438"},{"id":"R2949","pred":"arg2Of","subj":"T3442","obj":"T3425"},{"id":"R2950","pred":"arg1Of","subj":"T3442","obj":"T3441"},{"id":"R2951","pred":"arg2Of","subj":"T3445","obj":"T3442"},{"id":"R2952","pred":"arg1Of","subj":"T3445","obj":"T3443"},{"id":"R2953","pred":"arg1Of","subj":"T3445","obj":"T3444"},{"id":"R2954","pred":"arg1Of","subj":"T3445","obj":"T3446"},{"id":"R2955","pred":"arg2Of","subj":"T3448","obj":"T3446"},{"id":"R2956","pred":"arg1Of","subj":"T3448","obj":"T3447"},{"id":"R2957","pred":"arg1Of","subj":"T3448","obj":"T3449"},{"id":"R2958","pred":"arg3Of","subj":"T3450","obj":"T3404"},{"id":"R2959","pred":"modOf","subj":"T3451","obj":"T3392"},{"id":"R2960","pred":"arg1Of","subj":"T3451","obj":"T3397"},{"id":"R2961","pred":"arg2Of","subj":"T3451","obj":"T3399"},{"id":"R2962","pred":"arg2Of","subj":"T3452","obj":"T3451"},{"id":"R2963","pred":"arg1Of","subj":"T3452","obj":"T3453"},{"id":"R2964","pred":"arg1Of","subj":"T3452","obj":"T3456"},{"id":"R2965","pred":"arg2Of","subj":"T3455","obj":"T3453"},{"id":"R2966","pred":"arg1Of","subj":"T3455","obj":"T3454"},{"id":"R2967","pred":"arg2Of","subj":"T3458","obj":"T3456"},{"id":"R2968","pred":"arg1Of","subj":"T3458","obj":"T3457"},{"id":"R2969","pred":"arg1Of","subj":"T3458","obj":"T3459"},{"id":"R2970","pred":"arg2Of","subj":"T3460","obj":"T3459"},{"id":"R2971","pred":"arg1Of","subj":"T3462","obj":"T3461"},{"id":"R2972","pred":"arg1Of","subj":"T3462","obj":"T3463"},{"id":"R2973","pred":"arg2Of","subj":"T3462","obj":"T3464"},{"id":"R2974","pred":"arg2Of","subj":"T3464","obj":"T3463"},{"id":"R2975","pred":"arg1Of","subj":"T3470","obj":"T3464"},{"id":"R2976","pred":"arg2Of","subj":"T3470","obj":"T3465"},{"id":"R2977","pred":"arg1Of","subj":"T3470","obj":"T3466"},{"id":"R2978","pred":"arg1Of","subj":"T3470","obj":"T3467"},{"id":"R2979","pred":"arg1Of","subj":"T3470","obj":"T3468"},{"id":"R2980","pred":"arg1Of","subj":"T3470","obj":"T3469"},{"id":"R2981","pred":"arg1Of","subj":"T3470","obj":"T3471"},{"id":"R2982","pred":"arg1Of","subj":"T3472","obj":"T3473"},{"id":"R2983","pred":"arg2Of","subj":"T3473","obj":"T3471"},{"id":"R2984","pred":"arg1Of","subj":"T3473","obj":"T3475"},{"id":"R2985","pred":"arg2Of","subj":"T3474","obj":"T3473"},{"id":"R2986","pred":"arg2Of","subj":"T3476","obj":"T3475"},{"id":"R2987","pred":"arg3Of","subj":"T3477","obj":"T3471"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA)."}

{"project":"GO-CC","denotations":[{"id":"T3677","span":{"begin":33,"end":38},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA)."}

{"project":"sentences","denotations":[{"id":"T3376","span":{"begin":0,"end":376},"obj":"Sentence"},{"id":"T85","span":{"begin":0,"end":279},"obj":"Sentence"},{"id":"T86","span":{"begin":280,"end":376},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA)."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T4032","span":{"begin":359,"end":369},"obj":"Entity"},{"id":"T4031","span":{"begin":277,"end":287},"obj":"Protein"},{"id":"T4025","span":{"begin":152,"end":161},"obj":"Entity"},{"id":"T4023","span":{"begin":117,"end":138},"obj":"Entity"},{"id":"T4022","span":{"begin":175,"end":184},"obj":"Entity"},{"id":"T4018","span":{"begin":190,"end":198},"obj":"Protein"},{"id":"T4014","span":{"begin":33,"end":38},"obj":"Entity"},{"id":"T4013","span":{"begin":10,"end":32},"obj":"Protein"},{"id":"T4012","span":{"begin":107,"end":115},"obj":"Protein"},{"id":"T4010","span":{"begin":350,"end":370},"obj":"Entity"},{"id":"T4008","span":{"begin":81,"end":116},"obj":"Entity"},{"id":"T4005","span":{"begin":336,"end":343},"obj":"Entity"},{"id":"T4002","span":{"begin":190,"end":217},"obj":"Entity"},{"id":"T3999","span":{"begin":128,"end":137},"obj":"Entity"},{"id":"T3998","span":{"begin":163,"end":173},"obj":"Entity"}],"text":"To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA)."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T3704","span":{"begin":16,"end":23},"obj":"NN"},{"id":"T3703","span":{"begin":10,"end":15},"obj":"JJ"},{"id":"T3702","span":{"begin":3,"end":9},"obj":"VB"},{"id":"T3701","span":{"begin":0,"end":2},"obj":"TO"},{"id":"T3787","span":{"begin":371,"end":374},"obj":"NNP"},{"id":"T3786","span":{"begin":369,"end":370},"obj":","},{"id":"T3785","span":{"begin":367,"end":369},"obj":"NNP"},{"id":"T3784","span":{"begin":365,"end":366},"obj":","},{"id":"T3783","span":{"begin":359,"end":365},"obj":"NNP"},{"id":"T3782","span":{"begin":358,"end":359},"obj":"-LRB-"},{"id":"T3781","span":{"begin":350,"end":357},"obj":"NNP"},{"id":"T3780","span":{"begin":344,"end":349},"obj":"NNP"},{"id":"T3779","span":{"begin":336,"end":343},"obj":"NNP"},{"id":"T3778","span":{"begin":331,"end":335},"obj":"NNP"},{"id":"T3777","span":{"begin":321,"end":330},"obj":"NNP"},{"id":"T3776","span":{"begin":318,"end":320},"obj":"IN"},{"id":"T3775","span":{"begin":308,"end":317},"obj":"VBN"},{"id":"T3774","span":{"begin":303,"end":307},"obj":"VBD"},{"id":"T3773","span":{"begin":288,"end":302},"obj":"NNS"},{"id":"T3772","span":{"begin":280,"end":287},"obj":"NNP"},{"id":"T3771","span":{"begin":277,"end":279},"obj":"NNP"},{"id":"T3770","span":{"begin":276,"end":277},"obj":"CD"},{"id":"T3769","span":{"begin":275,"end":276},"obj":"CD"},{"id":"T3768","span":{"begin":272,"end":274},"obj":"IN"},{"id":"T3767","span":{"begin":268,"end":271},"obj":"NN"},{"id":"T3766","span":{"begin":265,"end":267},"obj":"CD"},{"id":"T3765","span":{"begin":261,"end":264},"obj":"IN"},{"id":"T3764","span":{"begin":259,"end":260},"obj":"NN"},{"id":"T3763","span":{"begin":252,"end":258},"obj":"CD"},{"id":"T3762","span":{"begin":249,"end":251},"obj":"IN"},{"id":"T3761","span":{"begin":237,"end":248},"obj":"VBN"},{"id":"T3760","span":{"begin":233,"end":236},"obj":"CC"},{"id":"T3759","span":{"begin":231,"end":232},"obj":"-RRB-"},{"id":"T3758","span":{"begin":228,"end":231},"obj":"CD"},{"id":"T3757","span":{"begin":225,"end":227},"obj":"NNP"},{"id":"T3756","span":{"begin":221,"end":224},"obj":"NNP"},{"id":"T3755","span":{"begin":218,"end":220},"obj":"IN"},{"id":"T3754","span":{"begin":209,"end":217},"obj":"NN"},{"id":"T3753","span":{"begin":199,"end":208},"obj":"NN"},{"id":"T3752","span":{"begin":190,"end":198},"obj":"NN"},{"id":"T3751","span":{"begin":186,"end":189},"obj":"CC"},{"id":"T3750","span":{"begin":184,"end":185},"obj":","},{"id":"T3749","span":{"begin":180,"end":184},"obj":"NNP"},{"id":"T3748","span":{"begin":177,"end":179},"obj":"NNP"},{"id":"T3747","span":{"begin":175,"end":176},"obj":"CD"},{"id":"T3746","span":{"begin":173,"end":174},"obj":","},{"id":"T3745","span":{"begin":170,"end":173},"obj":"NNP"},{"id":"T3744","span":{"begin":167,"end":169},"obj":"NNP"},{"id":"T3743","span":{"begin":163,"end":166},"obj":"CD"},{"id":"T3742","span":{"begin":161,"end":162},"obj":","},{"id":"T3741","span":{"begin":158,"end":161},"obj":"NNP"},{"id":"T3740","span":{"begin":155,"end":157},"obj":"NNP"},{"id":"T3739","span":{"begin":152,"end":154},"obj":"CD"},{"id":"T3738","span":{"begin":150,"end":151},"obj":","},{"id":"T3737","span":{"begin":144,"end":150},"obj":"NNP"},{"id":"T3736","span":{"begin":141,"end":143},"obj":"NNP"},{"id":"T3735","span":{"begin":139,"end":140},"obj":"CD"},{"id":"T3734","span":{"begin":137,"end":138},"obj":","},{"id":"T3733","span":{"begin":133,"end":137},"obj":"NNP"},{"id":"T3732","span":{"begin":130,"end":132},"obj":"NNP"},{"id":"T3731","span":{"begin":128,"end":129},"obj":"CD"},{"id":"T3730","span":{"begin":126,"end":127},"obj":","},{"id":"T3729","span":{"begin":122,"end":126},"obj":"NNP"},{"id":"T3728","span":{"begin":119,"end":121},"obj":"NNP"},{"id":"T3727","span":{"begin":117,"end":118},"obj":"CD"},{"id":"T3726","span":{"begin":115,"end":116},"obj":","},{"id":"T3725","span":{"begin":112,"end":115},"obj":"NNP"},{"id":"T3724","span":{"begin":110,"end":111},"obj":"NN"},{"id":"T3723","span":{"begin":107,"end":110},"obj":"CD"},{"id":"T3722","span":{"begin":105,"end":106},"obj":","},{"id":"T3721","span":{"begin":93,"end":105},"obj":"NN"},{"id":"T3720","span":{"begin":86,"end":92},"obj":"NN"},{"id":"T3719","span":{"begin":84,"end":85},"obj":"NN"},{"id":"T3718","span":{"begin":81,"end":84},"obj":"CD"},{"id":"T3717","span":{"begin":79,"end":80},"obj":","},{"id":"T3716","span":{"begin":75,"end":79},"obj":"CD"},{"id":"T3715","span":{"begin":73,"end":74},"obj":"NN"},{"id":"T3714","span":{"begin":72,"end":73},"obj":"CD"},{"id":"T3713","span":{"begin":71,"end":72},"obj":"-LRB-"},{"id":"T3712","span":{"begin":64,"end":70},"obj":"NN"},{"id":"T3711","span":{"begin":59,"end":63},"obj":"NNP"},{"id":"T3710","span":{"begin":56,"end":58},"obj":"IN"},{"id":"T3709","span":{"begin":44,"end":55},"obj":"VBN"},{"id":"T3708","span":{"begin":39,"end":43},"obj":"VBD"},{"id":"T3707","span":{"begin":33,"end":38},"obj":"NNS"},{"id":"T3706","span":{"begin":31,"end":32},"obj":","},{"id":"T3705","span":{"begin":24,"end":31},"obj":"VBZ"},{"id":"T3789","span":{"begin":375,"end":376},"obj":"."},{"id":"T3788","span":{"begin":374,"end":375},"obj":"-RRB-"}],"relations":[{"id":"R3195","pred":"aux","subj":"T3701","obj":"T3702"},{"id":"R3196","pred":"ROOT","subj":"T3702","obj":"T3702"},{"id":"R3197","pred":"amod","subj":"T3703","obj":"T3704"},{"id":"R3198","pred":"dobj","subj":"T3704","obj":"T3702"},{"id":"R3199","pred":"advmod","subj":"T3705","obj":"T3709"},{"id":"R3200","pred":"punct","subj":"T3706","obj":"T3709"},{"id":"R3201","pred":"nsubjpass","subj":"T3707","obj":"T3709"},{"id":"R3202","pred":"auxpass","subj":"T3708","obj":"T3709"},{"id":"R3203","pred":"advcl","subj":"T3709","obj":"T3702"},{"id":"R3204","pred":"prep","subj":"T3710","obj":"T3709"},{"id":"R3205","pred":"compound","subj":"T3711","obj":"T3712"},{"id":"R3206","pred":"pobj","subj":"T3712","obj":"T3710"},{"id":"R3207","pred":"punct","subj":"T3713","obj":"T3715"},{"id":"R3208","pred":"nummod","subj":"T3714","obj":"T3715"},{"id":"R3209","pred":"nsubjpass","subj":"T3715","obj":"T3775"},{"id":"R3210","pred":"nummod","subj":"T3716","obj":"T3721"},{"id":"R3211","pred":"punct","subj":"T3717","obj":"T3716"},{"id":"R3212","pred":"nummod","subj":"T3718","obj":"T3719"},{"id":"R3213","pred":"nmod","subj":"T3719","obj":"T3721"},{"id":"R3214","pred":"compound","subj":"T3720","obj":"T3721"},{"id":"R3215","pred":"appos","subj":"T3721","obj":"T3715"},{"id":"R3216","pred":"punct","subj":"T3722","obj":"T3721"},{"id":"R3217","pred":"nummod","subj":"T3723","obj":"T3724"},{"id":"R3218","pred":"appos","subj":"T3724","obj":"T3715"},{"id":"R3219","pred":"npadvmod","subj":"T3725","obj":"T3715"},{"id":"R3220","pred":"punct","subj":"T3726","obj":"T3725"},{"id":"R3221","pred":"nummod","subj":"T3727","obj":"T3729"},{"id":"R3222","pred":"compound","subj":"T3728","obj":"T3729"},{"id":"R3223","pred":"appos","subj":"T3729","obj":"T3725"},{"id":"R3224","pred":"punct","subj":"T3730","obj":"T3725"},{"id":"R3225","pred":"nummod","subj":"T3731","obj":"T3733"},{"id":"R3226","pred":"compound","subj":"T3732","obj":"T3733"},{"id":"R3227","pred":"appos","subj":"T3733","obj":"T3725"},{"id":"R3228","pred":"punct","subj":"T3734","obj":"T3725"},{"id":"R3229","pred":"nummod","subj":"T3735","obj":"T3737"},{"id":"R3230","pred":"compound","subj":"T3736","obj":"T3737"},{"id":"R3231","pred":"appos","subj":"T3737","obj":"T3725"},{"id":"R3232","pred":"punct","subj":"T3738","obj":"T3725"},{"id":"R3233","pred":"nummod","subj":"T3739","obj":"T3741"},{"id":"R3234","pred":"compound","subj":"T3740","obj":"T3741"},{"id":"R3235","pred":"appos","subj":"T3741","obj":"T3725"},{"id":"R3236","pred":"punct","subj":"T3742","obj":"T3725"},{"id":"R3237","pred":"nummod","subj":"T3743","obj":"T3745"},{"id":"R3238","pred":"compound","subj":"T3744","obj":"T3745"},{"id":"R3239","pred":"appos","subj":"T3745","obj":"T3725"},{"id":"R3240","pred":"punct","subj":"T3746","obj":"T3725"},{"id":"R3241","pred":"nummod","subj":"T3747","obj":"T3749"},{"id":"R3242","pred":"compound","subj":"T3748","obj":"T3749"},{"id":"R3243","pred":"appos","subj":"T3749","obj":"T3725"},{"id":"R3244","pred":"punct","subj":"T3750","obj":"T3725"},{"id":"R3245","pred":"cc","subj":"T3751","obj":"T3715"},{"id":"R3246","pred":"compound","subj":"T3752","obj":"T3753"},{"id":"R3247","pred":"compound","subj":"T3753","obj":"T3754"},{"id":"R3248","pred":"conj","subj":"T3754","obj":"T3715"},{"id":"R3249","pred":"prep","subj":"T3755","obj":"T3715"},{"id":"R3250","pred":"pobj","subj":"T3756","obj":"T3755"},{"id":"R3251","pred":"quantmod","subj":"T3757","obj":"T3758"},{"id":"R3252","pred":"dep","subj":"T3758","obj":"T3715"},{"id":"R3253","pred":"punct","subj":"T3759","obj":"T3758"},{"id":"R3254","pred":"cc","subj":"T3760","obj":"T3715"},{"id":"R3255","pred":"conj","subj":"T3761","obj":"T3715"},{"id":"R3256","pred":"prep","subj":"T3762","obj":"T3761"},{"id":"R3257","pred":"nummod","subj":"T3763","obj":"T3764"},{"id":"R3258","pred":"pobj","subj":"T3764","obj":"T3762"},{"id":"R3259","pred":"prep","subj":"T3765","obj":"T3761"},{"id":"R3260","pred":"nummod","subj":"T3766","obj":"T3767"},{"id":"R3261","pred":"pobj","subj":"T3767","obj":"T3765"},{"id":"R3262","pred":"prep","subj":"T3768","obj":"T3767"},{"id":"R3263","pred":"nummod","subj":"T3769","obj":"T3770"},{"id":"R3264","pred":"nummod","subj":"T3770","obj":"T3773"},{"id":"R3265","pred":"nmod","subj":"T3771","obj":"T3773"},{"id":"R3266","pred":"compound","subj":"T3772","obj":"T3773"},{"id":"R3267","pred":"pobj","subj":"T3773","obj":"T3768"},{"id":"R3268","pred":"auxpass","subj":"T3774","obj":"T3775"},{"id":"R3269","pred":"conj","subj":"T3775","obj":"T3702"},{"id":"R3270","pred":"agent","subj":"T3776","obj":"T3775"},{"id":"R3271","pred":"compound","subj":"T3777","obj":"T3781"},{"id":"R3272","pred":"compound","subj":"T3778","obj":"T3779"},{"id":"R3273","pred":"compound","subj":"T3779","obj":"T3781"},{"id":"R3274","pred":"compound","subj":"T3780","obj":"T3781"},{"id":"R3275","pred":"pobj","subj":"T3781","obj":"T3776"},{"id":"R3276","pred":"punct","subj":"T3782","obj":"T3783"},{"id":"R3277","pred":"appos","subj":"T3783","obj":"T3781"},{"id":"R3278","pred":"punct","subj":"T3784","obj":"T3783"},{"id":"R3279","pred":"prep","subj":"T3785","obj":"T3783"},{"id":"R3280","pred":"punct","subj":"T3786","obj":"T3785"},{"id":"R3281","pred":"npadvmod","subj":"T3787","obj":"T3785"},{"id":"R3282","pred":"punct","subj":"T3788","obj":"T3783"},{"id":"R3283","pred":"punct","subj":"T3789","obj":"T3775"}],"text":"To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA)."}