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PMC:3317373 / 10566-11434 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4496 550-552 TO denotes to
T4495 545-549 NN denotes MMP9
T4494 541-544 DT denotes the
T4493 534-540 VB denotes enable
T4492 531-533 TO denotes to
T4491 526-530 NN denotes 37°C
T4490 523-525 IN denotes at
T4489 521-522 NN denotes h
T4488 518-520 CD denotes 40
T4487 514-517 IN denotes for
T4486 507-513 NN denotes buffer
T4485 497-506 NN denotes substrate
T4484 494-496 IN denotes in
T4483 484-493 VB denotes incubated
T4482 479-483 RB denotes then
T4481 475-478 CC denotes and
T4480 469-474 NN denotes X-100
T4479 462-468 NN denotes Triton
T4478 460-461 NN denotes %
T4477 457-460 CD denotes 2.5
T4476 454-456 IN denotes in
T4475 443-453 NN denotes incubation
T4474 440-442 IN denotes by
T4473 430-439 VB denotes renatured
T4472 425-429 VB denotes were
T4471 416-424 NN denotes proteins
T4470 412-415 DT denotes the
T4469 407-411 RB denotes Then
T4468 390-405 NN denotes electrophoresis
T4467 387-389 IN denotes by
T4466 377-386 VB denotes separated
T4465 372-376 VB denotes were
T4464 363-371 NN denotes extracts
T4463 355-362 NN denotes protein
T4462 345-354 JJ denotes cytosolic
T4461 342-344 NN denotes μg
T4460 339-341 CD denotes 40
T4459 337-338 -COMMA- denotes ,
T4458 330-337 RB denotes Briefly
T4457 327-328 -RRB- denotes )
T4456 324-327 NNP denotes USA
T4455 322-323 -COMMA- denotes ,
T4454 320-322 NNP denotes MA
T4453 318-319 -COMMA- denotes ,
T4452 315-318 NNP denotes Inc
T4451 303-314 NNP denotes Scientifics
T4450 296-302 NNP denotes Genmed
T4449 295-296 -LRB- denotes (
T4448 282-294 NN denotes instructions
T4447 279-281 POS denotes 's
T4446 267-279 NN denotes manufacturer
T4445 263-266 DT denotes the
T4444 260-262 TO denotes to
T4443 250-259 VB denotes according
T4442 240-249 VB denotes performed
T4441 236-239 VB denotes was
T4440 225-235 NN denotes zymography
T4439 217-224 NN denotes Gelatin
T4438 210-215 NN denotes –20°C
T4437 207-209 IN denotes at
T4436 200-206 VB denotes stored
T4435 196-199 CC denotes and
T4434 181-195 NN denotes centrifugation
T4433 178-180 IN denotes by
T4432 168-177 VB denotes separated
T4431 163-167 VB denotes were
T4430 154-162 NN denotes extracts
T4429 146-153 NNP denotes Soluble
T4428 138-145 NNP denotes Triton.
T4427 136-137 NN denotes %
T4426 132-136 CD denotes 0.01
T4425 128-131 CC denotes and
T4424 126-127 -COMMA- denotes ,
T4423 122-126 NN denotes NaN3
T4422 119-121 NN denotes mM
T4421 115-118 CD denotes 0.2
T4420 113-114 -COMMA- denotes ,
T4419 108-113 NN denotes CaCl2
T4418 105-107 NN denotes mM
T4417 103-104 CD denotes 5
T4416 101-102 -COMMA- denotes ,
T4415 97-101 NN denotes NaCl
T4414 94-96 NN denotes mM
T4413 90-93 CD denotes 150
T4412 88-89 -COMMA- denotes ,
T4411 87-88 -RRB- denotes )
T4410 84-87 CD denotes 7.4
T4409 81-83 NN denotes pH
T4408 80-81 -LRB- denotes (
T4407 71-79 NN denotes Tris-HCl
T4406 68-70 NN denotes mM
T4405 65-67 CD denotes 50
T4404 54-64 VB denotes containing
T4403 47-53 NN denotes buffer
T4402 41-46 NN denotes lysis
T4401 38-40 IN denotes in
T4400 26-37 VB denotes homogenized
T4399 21-25 VB denotes were
T4398 14-20 NN denotes groups
T4397 9-13 CD denotes four
T4396 6-8 IN denotes of
T4395 0-5 NN denotes Cells
T4549 861-867 NN denotes ImageJ
T4548 853-860 NN denotes program
T4547 844-852 NN denotes analysis
T4546 838-843 NN denotes image
T4545 833-837 IN denotes with
T4544 823-832 VB denotes performed
T4543 819-822 VB denotes was
T4542 811-818 NN denotes density
T4541 806-810 NN denotes band
T4540 801-805 NN denotes MMP9
T4539 798-800 IN denotes of
T4538 783-797 NN denotes Quantification
T4537 772-781 NN denotes substrate
T4536 768-771 DT denotes the
T4535 765-767 IN denotes of
T4534 759-764 NN denotes lysis
T4533 755-758 DT denotes the
T4532 745-754 VB denotes indicates
T4531 739-744 WDT denotes which
T4530 735-738 NN denotes gel
T4529 730-734 JJ denotes blue
T4528 727-729 IN denotes in
T4527 721-726 NN denotes bands
T4526 715-720 JJ denotes clear
T4525 712-714 IN denotes by
T4524 705-711 VB denotes showed
T4523 700-704 VB denotes were
T4522 689-699 NN denotes activities
T4521 677-688 JJ denotes Proteolytic
T4520 667-675 NN denotes methanol
T4519 665-666 NN denotes %
T4518 663-665 CD denotes 50
T4517 660-662 IN denotes in
T4516 650-659 VB denotes destained
T4515 646-649 CC denotes and
T4514 644-645 NN denotes h
T4513 642-643 CD denotes 1
T4512 638-641 IN denotes for
T4511 633-637 JJ denotes blue
T4510 623-632 NNP denotes Coomassie
T4509 618-622 IN denotes with
T4508 610-617 VB denotes stained
T4507 606-609 VB denotes was
T4506 602-605 NN denotes gel
T4505 597-601 DT denotes each
T4504 595-596 -COMMA- denotes ,
T4503 590-595 NN denotes water
T4502 587-589 IN denotes in
T4501 579-586 VB denotes rinsing
T4500 573-578 IN denotes After
T4499 564-571 NN denotes gelatin
T4498 560-563 DT denotes the
T4497 553-559 VB denotes cleave
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T4389 801-805 Protein denotes MMP9
T4388 545-549 Protein denotes MMP9

GO-BP

Id Subject Object Predicate Lexical cue
T4391 759-764 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4390 41-46 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

sentences

Id Subject Object Predicate Lexical cue
T4385 783-868 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T4384 677-782 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4383 573-676 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4382 407-572 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4381 330-406 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4380 0-329 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T109 0-145 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T110 146-216 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 217-329 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T112 330-406 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T113 407-572 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T114 573-676 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T115 677-782 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T116 783-868 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.

simple1

Id Subject Object Predicate Lexical cue
T4551 801-805 Protein denotes MMP9
T4550 545-549 Protein denotes MMP9

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4732 801-805 Protein denotes MMP9
T4731 545-549 Protein denotes MMP9

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4556 553-559 Protein_catabolism denotes cleave
T4555 801-805 Protein denotes MMP9
T4554 545-549 Protein denotes MMP9
R3872 T4554 T4556 themeOf MMP9,cleave

DLUT931

Id Subject Object Predicate Lexical cue
T4553 801-805 Protein denotes MMP9
T4552 545-549 Protein denotes MMP9

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4751 497-506 Entity denotes substrate
T4750 339-371 Entity denotes 40 μg cytosolic protein extracts
T4749 0-20 Entity denotes Cells of four groups
T4748 642-645 Entity denotes 1 h
T4747 457-474 Entity denotes 2.5% Triton X-100
T4746 146-162 Entity denotes Soluble extracts
T4745 541-549 Protein denotes the MMP9
T4744 115-144 Entity denotes 0.2 mM NaN3, and 0.01% Triton
T4743 132-144 Entity denotes 0.01% Triton
T4742 65-79 Entity denotes 50 mM Tris-HCl
T4741 730-781 Entity denotes blue gel which indicates the lysis of the substrate
T4740 90-101 Entity denotes 150 mM NaCl
T4739 597-605 Entity denotes each gel
T4738 801-805 Protein denotes MMP9
T4737 339-371 Protein denotes 40 μg cytosolic protein extracts
T4736 217-224 Entity denotes Gelatin
T4735 412-424 Protein denotes the proteins
T4734 65-79 Entity denotes 50 mM Tris-HCl
T4733 663-675 Entity denotes 50% methanol

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4729 867-868 . denotes .
T4728 861-867 NNP denotes ImageJ
T4727 853-860 NN denotes program
T4726 844-852 NN denotes analysis
T4725 838-843 NN denotes image
T4723 823-832 VBN denotes performed
T4722 819-822 VBD denotes was
T4721 811-818 NN denotes density
T4720 806-810 NN denotes band
T4719 801-805 NNP denotes MMP9
T4718 798-800 IN denotes of
T4717 783-797 NN denotes Quantification
T4716 781-782 . denotes .
T4715 772-781 NN denotes substrate
T4714 768-771 DT denotes the
T4713 765-767 IN denotes of
T4712 759-764 NN denotes lysis
T4711 755-758 DT denotes the
T4710 745-754 VBZ denotes indicates
T4709 739-744 WDT denotes which
T4708 735-738 NN denotes gel
T4707 730-734 JJ denotes blue
T4706 727-729 IN denotes in
T4705 721-726 NNS denotes bands
T4704 715-720 JJ denotes clear
T4703 712-714 IN denotes by
T4702 705-711 VBN denotes showed
T4701 700-704 VBD denotes were
T4700 689-699 NNS denotes activities
T4699 677-688 JJ denotes Proteolytic
T4698 675-676 . denotes .
T4697 667-675 NN denotes methanol
T4696 665-666 NN denotes %
T4695 663-665 CD denotes 50
T4694 660-662 IN denotes in
T4693 650-659 VBN denotes destained
T4692 646-649 CC denotes and
T4686 618-622 IN denotes with
T4685 610-617 VBN denotes stained
T4684 606-609 VBD denotes was
T4683 602-605 NN denotes gel
T4682 597-601 DT denotes each
T4652 460-461 NN denotes %
T4651 457-460 CD denotes 2.5
T4650 454-456 IN denotes in
T4649 443-453 NN denotes incubation
T4648 440-442 IN denotes by
T4647 430-439 VBN denotes renatured
T4646 425-429 VBD denotes were
T4645 416-424 NNS denotes proteins
T4644 412-415 DT denotes the
T4643 407-411 RB denotes Then
T4642 405-406 . denotes .
T4641 390-405 NN denotes electrophoresis
T4640 387-389 IN denotes by
T4639 377-386 VBN denotes separated
T4638 372-376 VBD denotes were
T4637 363-371 NNS denotes extracts
T4636 355-362 NN denotes protein
T4635 345-354 JJ denotes cytosolic
T4634 342-344 NN denotes μg
T4633 339-341 CD denotes 40
T4632 337-338 , denotes ,
T4631 330-337 RB denotes Briefly
T4630 328-329 . denotes .
T4629 327-328 -RRB- denotes )
T4628 324-327 NNP denotes USA
T4627 322-323 , denotes ,
T4626 320-322 NNP denotes MA
T4625 318-319 , denotes ,
T4624 315-318 NNP denotes Inc
T4623 303-314 NNP denotes Scientifics
T4622 296-302 NNP denotes Genmed
T4621 295-296 -LRB- denotes (
T4620 282-294 NNS denotes instructions
T4619 279-281 POS denotes 's
T4618 267-279 NN denotes manufacturer
T4617 263-266 DT denotes the
T4616 260-262 TO denotes to
T4615 250-259 VBG denotes according
T4614 240-249 VBN denotes performed
T4613 236-239 VBD denotes was
T4612 225-235 NN denotes zymography
T4611 217-224 NNP denotes Gelatin
T4610 214-216 NNP denotes C.
T4609 213-214 NN denotes °
T4608 211-213 CD denotes 20
T4607 207-209 IN denotes at
T4606 200-206 VBD denotes stored
T4605 196-199 CC denotes and
T4604 181-195 NN denotes centrifugation
T4603 178-180 IN denotes by
T4602 168-177 VBN denotes separated
T4601 163-167 VBD denotes were
T4600 154-162 NNS denotes extracts
T4599 146-153 JJ denotes Soluble
T4598 144-145 . denotes .
T4597 138-144 NNP denotes Triton
T4596 136-137 NN denotes %
T4595 132-136 CD denotes 0.01
T4594 128-131 CC denotes and
T4593 126-127 , denotes ,
T4592 122-126 NNP denotes NaN3
T4591 119-121 NNP denotes mM
T4590 115-118 CD denotes 0.2
T4589 113-114 , denotes ,
T4588 108-113 NNP denotes CaCl2
T4587 105-107 NNP denotes mM
T4586 103-104 CD denotes 5
T4585 101-102 , denotes ,
T4584 97-101 NNP denotes NaCl
T4583 94-96 NNP denotes mM
T4582 90-93 CD denotes 150
T4581 88-89 , denotes ,
T4580 87-88 -RRB- denotes )
T4579 84-87 CD denotes 7.4
T4578 81-83 NNP denotes pH
T4577 80-81 -LRB- denotes (
T4576 71-79 NNP denotes Tris-HCl
T4575 68-70 NNP denotes mM
T4574 65-67 CD denotes 50
T4573 54-64 VBG denotes containing
T4572 47-53 NN denotes buffer
T4571 41-46 NN denotes lysis
T4570 38-40 IN denotes in
T4569 26-37 VBN denotes homogenized
T4568 21-25 VBD denotes were
T4567 14-20 NNS denotes groups
T4566 9-13 CD denotes four
T4565 6-8 IN denotes of
T4564 0-5 NNP denotes Cells
T4691 644-645 NN denotes h
T4690 642-643 CD denotes 1
T4689 638-641 IN denotes for
T4688 633-637 JJ denotes blue
T4687 623-632 NNP denotes Coomassie
T4681 595-596 , denotes ,
T4680 590-595 NN denotes water
T4679 587-589 IN denotes in
T4678 579-586 VBG denotes rinsing
T4677 573-578 IN denotes After
T4676 571-572 . denotes .
T4675 564-571 NN denotes gelatin
T4674 560-563 DT denotes the
T4673 553-559 VB denotes cleave
T4672 550-552 TO denotes to
T4671 545-549 NNP denotes MMP9
T4670 541-544 DT denotes the
T4669 534-540 VB denotes enable
T4668 531-533 TO denotes to
T4667 529-530 NNP denotes C
T4666 528-529 CD denotes °
T4665 526-528 CD denotes 37
T4664 523-525 IN denotes at
T4663 521-522 NN denotes h
T4662 518-520 CD denotes 40
T4661 514-517 IN denotes for
T4660 507-513 NN denotes buffer
T4659 497-506 JJ denotes substrate
T4658 494-496 IN denotes in
T4657 484-493 VBD denotes incubated
T4656 479-483 RB denotes then
T4655 475-478 CC denotes and
T4654 469-474 NNP denotes X-100
T4653 462-468 NNP denotes Triton
T4724 833-837 IN denotes with
R3887 T4574 T4576 nummod 50,Tris-HCl
R3888 T4575 T4576 compound mM,Tris-HCl
R3889 T4576 T4573 dobj Tris-HCl,containing
R3890 T4577 T4578 punct (,pH
R3891 T4578 T4576 appos pH,Tris-HCl
R3892 T4579 T4578 nummod 7.4,pH
R3893 T4580 T4578 punct ),pH
R3894 T4581 T4576 punct ",",Tris-HCl
R3895 T4582 T4584 nummod 150,NaCl
R3896 T4583 T4584 compound mM,NaCl
R3897 T4584 T4576 conj NaCl,Tris-HCl
R3898 T4585 T4584 punct ",",NaCl
R3899 T4586 T4588 nummod 5,CaCl2
R3900 T4587 T4588 compound mM,CaCl2
R3901 T4588 T4584 conj CaCl2,NaCl
R3902 T4589 T4588 punct ",",CaCl2
R3903 T4590 T4592 nummod 0.2,NaN3
R3904 T4591 T4592 compound mM,NaN3
R3905 T4592 T4588 conj NaN3,CaCl2
R3906 T4593 T4592 punct ",",NaN3
R3907 T4594 T4592 cc and,NaN3
R3908 T4595 T4596 nummod 0.01,%
R3909 T4596 T4597 compound %,Triton
R3910 T4597 T4592 conj Triton,NaN3
R3911 T4598 T4569 punct .,homogenized
R3912 T4599 T4600 amod Soluble,extracts
R3913 T4600 T4602 nsubjpass extracts,separated
R3914 T4601 T4602 auxpass were,separated
R3915 T4602 T4602 ROOT separated,separated
R3916 T4603 T4602 agent by,separated
R3917 T4604 T4603 pobj centrifugation,by
R3918 T4605 T4602 cc and,separated
R3919 T4606 T4614 nsubjpass stored,performed
R3920 T4607 T4606 prep at,stored
R3921 T4608 T4609 nummod 20,°
R3922 T4609 T4612 compound °,zymography
R3923 T4610 T4612 compound C.,zymography
R3924 T4611 T4612 compound Gelatin,zymography
R3925 T4612 T4607 pobj zymography,at
R3926 T4613 T4614 auxpass was,performed
R3927 T4614 T4602 conj performed,separated
R3928 T4615 T4614 xcomp according,performed
R3929 T4616 T4615 prep to,according
R3930 T4617 T4618 det the,manufacturer
R3931 T4618 T4620 poss manufacturer,instructions
R3932 T4619 T4618 case 's,manufacturer
R3933 T4620 T4616 pobj instructions,to
R3934 T4621 T4624 punct (,Inc
R3935 T4622 T4624 compound Genmed,Inc
R3936 T4623 T4624 compound Scientifics,Inc
R3937 T4624 T4620 appos Inc,instructions
R3938 T4625 T4624 punct ",",Inc
R3939 T4626 T4624 conj MA,Inc
R3940 T4627 T4626 punct ",",MA
R3941 T4628 T4626 appos USA,MA
R3942 T4629 T4626 punct ),MA
R3943 T4630 T4614 punct .,performed
R3944 T4631 T4639 advmod Briefly,separated
R3945 T4632 T4639 punct ",",separated
R3946 T4633 T4637 nummod 40,extracts
R3947 T4634 T4637 compound μg,extracts
R3948 T4635 T4636 compound cytosolic,protein
R3949 T4636 T4637 compound protein,extracts
R3950 T4637 T4639 nsubjpass extracts,separated
R3951 T4638 T4639 auxpass were,separated
R3952 T4639 T4639 ROOT separated,separated
R3953 T4640 T4639 prep by,separated
R3954 T4641 T4640 pobj electrophoresis,by
R3955 T4642 T4639 punct .,separated
R3956 T4643 T4647 advmod Then,renatured
R3957 T4644 T4645 det the,proteins
R3958 T4645 T4647 nsubjpass proteins,renatured
R3959 T4646 T4647 auxpass were,renatured
R3960 T4647 T4647 ROOT renatured,renatured
R3961 T4648 T4647 agent by,renatured
R3962 T4649 T4648 pobj incubation,by
R3963 T4650 T4647 prep in,renatured
R3975 T4662 T4663 nummod 40,h
R3976 T4663 T4661 pobj h,for
R3977 T4664 T4657 prep at,incubated
R3978 T4665 T4666 nummod 37,°
R3979 T4666 T4664 pobj °,at
R3980 T4667 T4657 conj C,incubated
R3981 T4668 T4669 aux to,enable
R3982 T4669 T4657 advcl enable,incubated
R3983 T4670 T4671 det the,MMP9
R3984 T4671 T4669 dobj MMP9,enable
R3985 T4672 T4673 aux to,cleave
R3986 T4673 T4669 xcomp cleave,enable
R3987 T4674 T4675 det the,gelatin
R3988 T4675 T4673 dobj gelatin,cleave
R3989 T4676 T4647 punct .,renatured
R3990 T4677 T4685 prep After,stained
R3991 T4678 T4677 pcomp rinsing,After
R3992 T4679 T4678 prep in,rinsing
R3993 T4680 T4679 pobj water,in
R3994 T4681 T4685 punct ",",stained
R3995 T4682 T4683 det each,gel
R3996 T4683 T4685 nsubjpass gel,stained
R3997 T4684 T4685 auxpass was,stained
R3998 T4685 T4685 ROOT stained,stained
R3999 T4686 T4685 prep with,stained
R4000 T4687 T4688 compound Coomassie,blue
R4001 T4688 T4686 pobj blue,with
R4002 T4689 T4688 prep for,blue
R4003 T4690 T4691 nummod 1,h
R4004 T4691 T4689 pobj h,for
R4005 T4692 T4691 cc and,h
R4006 T4693 T4688 conj destained,blue
R4007 T4694 T4693 prep in,destained
R4008 T4695 T4696 nummod 50,%
R4009 T4696 T4697 compound %,methanol
R4010 T4697 T4694 pobj methanol,in
R4011 T4698 T4685 punct .,stained
R4012 T4699 T4700 amod Proteolytic,activities
R4013 T4700 T4702 nsubjpass activities,showed
R4014 T4701 T4702 auxpass were,showed
R4015 T4702 T4702 ROOT showed,showed
R4016 T4703 T4702 agent by,showed
R4017 T4704 T4705 amod clear,bands
R4018 T4705 T4703 pobj bands,by
R4019 T4706 T4705 prep in,bands
R4020 T4707 T4708 amod blue,gel
R4021 T4708 T4706 pobj gel,in
R4022 T4709 T4710 nsubj which,indicates
R4023 T4710 T4702 advcl indicates,showed
R4024 T4711 T4712 det the,lysis
R4025 T4712 T4710 dobj lysis,indicates
R4026 T4713 T4712 prep of,lysis
R4027 T4714 T4715 det the,substrate
R4028 T4715 T4713 pobj substrate,of
R4029 T4716 T4702 punct .,showed
R4030 T4717 T4723 nsubjpass Quantification,performed
R4031 T4718 T4717 prep of,Quantification
R3877 T4564 T4569 nsubjpass Cells,homogenized
R3878 T4565 T4564 prep of,Cells
R3879 T4566 T4567 nummod four,groups
R3880 T4567 T4565 pobj groups,of
R3881 T4568 T4569 auxpass were,homogenized
R3882 T4569 T4569 ROOT homogenized,homogenized
R3883 T4570 T4569 prep in,homogenized
R3884 T4571 T4572 compound lysis,buffer
R3885 T4572 T4570 pobj buffer,in
R3886 T4573 T4572 acl containing,buffer
R3964 T4651 T4652 nummod 2.5,%
R3965 T4652 T4654 compound %,X-100
R3966 T4653 T4654 compound Triton,X-100
R3967 T4654 T4650 pobj X-100,in
R3968 T4655 T4647 cc and,renatured
R3969 T4656 T4657 advmod then,incubated
R3970 T4657 T4647 conj incubated,renatured
R3971 T4658 T4657 prep in,incubated
R3972 T4659 T4660 compound substrate,buffer
R3973 T4660 T4658 pobj buffer,in
R3974 T4661 T4660 prep for,buffer
R4032 T4719 T4721 compound MMP9,density
R4033 T4720 T4721 compound band,density
R4034 T4721 T4718 pobj density,of
R4035 T4722 T4723 auxpass was,performed
R4036 T4723 T4723 ROOT performed,performed
R4037 T4724 T4723 prep with,performed
R4038 T4725 T4726 compound image,analysis
R4039 T4726 T4727 compound analysis,program
R4040 T4727 T4728 compound program,ImageJ
R4041 T4728 T4724 pobj ImageJ,with
R4042 T4729 T4723 punct .,performed

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4559 545-549 Protein denotes MMP9

testone

Id Subject Object Predicate Lexical cue
T4373 801-805 Protein denotes MMP9
T4372 545-549 Protein denotes MMP9

test3

Id Subject Object Predicate Lexical cue
T4377 801-805 Protein denotes MMP9
T4376 545-549 Protein denotes MMP9
T4375 801-805 Protein denotes MMP9
T4374 545-549 Protein denotes MMP9