PMC:3312845 / 235-1817 JSONTXT

{"project":"pmc-enju-pas","denotations":[{"id":"T457","span":{"begin":1577,"end":1581},"obj":"NN"},{"id":"T456","span":{"begin":1557,"end":1576},"obj":"JJ"},{"id":"T455","span":{"begin":1553,"end":1556},"obj":"CC"},{"id":"T454","span":{"begin":1540,"end":1552},"obj":"NN"},{"id":"T453","span":{"begin":1529,"end":1539},"obj":"NN"},{"id":"T452","span":{"begin":1519,"end":1528},"obj":"JJ"},{"id":"T451","span":{"begin":1516,"end":1518},"obj":"IN"},{"id":"T450","span":{"begin":1508,"end":1515},"obj":"VB"},{"id":"T449","span":{"begin":1502,"end":1507},"obj":"NN"},{"id":"T448","span":{"begin":1492,"end":1501},"obj":"JJ"},{"id":"T447","span":{"begin":1490,"end":1491},"obj":"-COMMA-"},{"id":"T446","span":{"begin":1478,"end":1490},"obj":"RB"},{"id":"T445","span":{"begin":1471,"end":1476},"obj":"NN"},{"id":"T444","span":{"begin":1461,"end":1470},"obj":"JJ"},{"id":"T443","span":{"begin":1450,"end":1460},"obj":"JJ"},{"id":"T442","span":{"begin":1447,"end":1449},"obj":"IN"},{"id":"T441","span":{"begin":1438,"end":1446},"obj":"VB"},{"id":"T440","span":{"begin":1435,"end":1437},"obj":"VB"},{"id":"T439","span":{"begin":1431,"end":1434},"obj":"MD"},{"id":"T438","span":{"begin":1415,"end":1430},"obj":"NN"},{"id":"T437","span":{"begin":1409,"end":1414},"obj":"NN"},{"id":"T436","span":{"begin":1405,"end":1408},"obj":"CD"},{"id":"T435","span":{"begin":1397,"end":1404},"obj":"NN"},{"id":"T434","span":{"begin":1382,"end":1396},"obj":"JJ"},{"id":"T433","span":{"begin":1378,"end":1381},"obj":"IN"},{"id":"T432","span":{"begin":1368,"end":1377},"obj":"NN"},{"id":"T431","span":{"begin":1363,"end":1367},"obj":"NN"},{"id":"T430","span":{"begin":1351,"end":1362},"obj":"JJ"},{"id":"T429","span":{"begin":1348,"end":1350},"obj":"IN"},{"id":"T428","span":{"begin":1336,"end":1347},"obj":"NN"},{"id":"T427","span":{"begin":1331,"end":1335},"obj":"IN"},{"id":"T426","span":{"begin":1323,"end":1330},"obj":"VB"},{"id":"T425","span":{"begin":1314,"end":1322},"obj":"NN"},{"id":"T424","span":{"begin":1308,"end":1313},"obj":"DT"},{"id":"T423","span":{"begin":1297,"end":1307},"obj":"NN"},{"id":"T422","span":{"begin":1292,"end":1294},"obj":"NN"},{"id":"T421","span":{"begin":1288,"end":1291},"obj":"DT"},{"id":"T420","span":{"begin":1285,"end":1287},"obj":"IN"},{"id":"T419","span":{"begin":1272,"end":1284},"obj":"NN"},{"id":"T418","span":{"begin":1261,"end":1271},"obj":"NN"},{"id":"T417","span":{"begin":1250,"end":1260},"obj":"JJ"},{"id":"T416","span":{"begin":1242,"end":1249},"obj":"VB"},{"id":"T415","span":{"begin":1233,"end":1241},"obj":"NN"},{"id":"T414","span":{"begin":1224,"end":1232},"obj":"NN"},{"id":"T413","span":{"begin":1222,"end":1223},"obj":"-COMMA-"},{"id":"T412","span":{"begin":1211,"end":1222},"obj":"RB"},{"id":"T411","span":{"begin":1204,"end":1209},"obj":"NN"},{"id":"T410","span":{"begin":1192,"end":1203},"obj":"JJ"},{"id":"T409","span":{"begin":1189,"end":1191},"obj":"IN"},{"id":"T408","span":{"begin":1173,"end":1188},"obj":"NN"},{"id":"T407","span":{"begin":1162,"end":1172},"obj":"NN"},{"id":"T406","span":{"begin":1159,"end":1161},"obj":"IN"},{"id":"T405","span":{"begin":1148,"end":1158},"obj":"NN"},{"id":"T404","span":{"begin":1144,"end":1147},"obj":"IN"},{"id":"T403","span":{"begin":1136,"end":1143},"obj":"NN"},{"id":"T402","span":{"begin":1127,"end":1135},"obj":"JJ"},{"id":"T401","span":{"begin":1123,"end":1126},"obj":"CC"},{"id":"T400","span":{"begin":1117,"end":1122},"obj":"NN"},{"id":"T399","span":{"begin":1107,"end":1116},"obj":"JJ"},{"id":"T398","span":{"begin":1096,"end":1106},"obj":"JJ"},{"id":"T397","span":{"begin":1085,"end":1095},"obj":"VB"},{"id":"T396","span":{"begin":1076,"end":1084},"obj":"NN"},{"id":"T395","span":{"begin":1074,"end":1075},"obj":"-RRB-"},{"id":"T394","span":{"begin":1066,"end":1074},"obj":"NN"},{"id":"T393","span":{"begin":1065,"end":1066},"obj":"-LRB-"},{"id":"T392","span":{"begin":1056,"end":1064},"obj":"NN"},{"id":"T391","span":{"begin":1052,"end":1055},"obj":"NN"},{"id":"T390","span":{"begin":1048,"end":1051},"obj":"NN"},{"id":"T389","span":{"begin":1040,"end":1047},"obj":"JJ"},{"id":"T388","span":{"begin":1037,"end":1039},"obj":"IN"},{"id":"T387","span":{"begin":1031,"end":1036},"obj":"NN"},{"id":"T386","span":{"begin":1028,"end":1030},"obj":"IN"},{"id":"T385","span":{"begin":1013,"end":1027},"obj":"NN"},{"id":"T384","span":{"begin":1010,"end":1011},"obj":"-RRB-"},{"id":"T383","span":{"begin":1003,"end":1010},"obj":"NN"},{"id":"T382","span":{"begin":995,"end":1002},"obj":"NN"},{"id":"T381","span":{"begin":983,"end":994},"obj":"JJ"},{"id":"T380","span":{"begin":980,"end":982},"obj":"DT"},{"id":"T379","span":{"begin":979,"end":980},"obj":"-LRB-"},{"id":"T378","span":{"begin":968,"end":978},"obj":"NN"},{"id":"T377","span":{"begin":961,"end":967},"obj":"NN"},{"id":"T376","span":{"begin":958,"end":960},"obj":"IN"},{"id":"T375","span":{"begin":946,"end":957},"obj":"VB"},{"id":"T374","span":{"begin":940,"end":945},"obj":"NN"},{"id":"T373","span":{"begin":928,"end":939},"obj":"JJ"},{"id":"T372","span":{"begin":925,"end":927},"obj":"IN"},{"id":"T371","span":{"begin":915,"end":924},"obj":"VB"},{"id":"T370","span":{"begin":911,"end":914},"obj":"VB"},{"id":"T369","span":{"begin":895,"end":910},"obj":"NN"},{"id":"T368","span":{"begin":884,"end":894},"obj":"NN"},{"id":"T367","span":{"begin":879,"end":883},"obj":"RB"},{"id":"T366","span":{"begin":877,"end":878},"obj":"-COMMA-"},{"id":"T365","span":{"begin":869,"end":877},"obj":"NN"},{"id":"T364","span":{"begin":866,"end":868},"obj":"IN"},{"id":"T363","span":{"begin":854,"end":864},"obj":"NN"},{"id":"T362","span":{"begin":851,"end":853},"obj":"IN"},{"id":"T361","span":{"begin":846,"end":850},"obj":"RB"},{"id":"T360","span":{"begin":843,"end":845},"obj":"RB"},{"id":"T359","span":{"begin":837,"end":842},"obj":"NN"},{"id":"T358","span":{"begin":825,"end":836},"obj":"JJ"},{"id":"T357","span":{"begin":822,"end":824},"obj":"IN"},{"id":"T356","span":{"begin":813,"end":821},"obj":"VB"},{"id":"T355","span":{"begin":809,"end":812},"obj":"VB"},{"id":"T354","span":{"begin":798,"end":808},"obj":"NN"},{"id":"T353","span":{"begin":789,"end":797},"obj":"NN"},{"id":"T352","span":{"begin":785,"end":788},"obj":"NN"},{"id":"T351","span":{"begin":781,"end":784},"obj":"NN"},{"id":"T350","span":{"begin":779,"end":780},"obj":"-COMMA-"},{"id":"T349","span":{"begin":771,"end":779},"obj":"NN"},{"id":"T348","span":{"begin":768,"end":770},"obj":"IN"},{"id":"T347","span":{"begin":750,"end":766},"obj":"NN"},{"id":"T346","span":{"begin":744,"end":749},"obj":"NN"},{"id":"T345","span":{"begin":737,"end":743},"obj":"JJ"},{"id":"T344","span":{"begin":730,"end":736},"obj":"VB"},{"id":"T343","span":{"begin":720,"end":729},"obj":"NN"},{"id":"T342","span":{"begin":710,"end":719},"obj":"VB"},{"id":"T282","span":{"begin":316,"end":321},"obj":"NN"},{"id":"T341","span":{"begin":705,"end":709},"obj":"RB"},{"id":"T340","span":{"begin":703,"end":704},"obj":"-COMMA-"},{"id":"T339","span":{"begin":701,"end":703},"obj":"NN"},{"id":"T338","span":{"begin":691,"end":700},"obj":"VB"},{"id":"T337","span":{"begin":683,"end":690},"obj":"NN"},{"id":"T336","span":{"begin":672,"end":680},"obj":"NN"},{"id":"T335","span":{"begin":666,"end":671},"obj":"NN"},{"id":"T334","span":{"begin":662,"end":665},"obj":"CC"},{"id":"T333","span":{"begin":656,"end":661},"obj":"NN"},{"id":"T332","span":{"begin":652,"end":655},"obj":"IN"},{"id":"T331","span":{"begin":644,"end":651},"obj":"NN"},{"id":"T330","span":{"begin":624,"end":643},"obj":"JJ"},{"id":"T329","span":{"begin":620,"end":623},"obj":"CC"},{"id":"T328","span":{"begin":611,"end":619},"obj":"NN"},{"id":"T327","span":{"begin":609,"end":610},"obj":"NN"},{"id":"T326","span":{"begin":597,"end":608},"obj":"JJ"},{"id":"T325","span":{"begin":587,"end":596},"obj":"VB"},{"id":"T324","span":{"begin":584,"end":586},"obj":"PRP"},{"id":"T323","span":{"begin":582,"end":583},"obj":"-COMMA-"},{"id":"T322","span":{"begin":572,"end":582},"obj":"RB"},{"id":"T321","span":{"begin":561,"end":570},"obj":"NN"},{"id":"T320","span":{"begin":558,"end":560},"obj":"NN"},{"id":"T319","span":{"begin":555,"end":557},"obj":"TO"},{"id":"T318","span":{"begin":549,"end":554},"obj":"JJ"},{"id":"T317","span":{"begin":547,"end":548},"obj":"-RRB-"},{"id":"T316","span":{"begin":539,"end":547},"obj":"NN"},{"id":"T315","span":{"begin":538,"end":539},"obj":"-LRB-"},{"id":"T314","span":{"begin":529,"end":537},"obj":"NN"},{"id":"T313","span":{"begin":525,"end":528},"obj":"NN"},{"id":"T312","span":{"begin":521,"end":524},"obj":"NN"},{"id":"T311","span":{"begin":513,"end":520},"obj":"JJ"},{"id":"T310","span":{"begin":509,"end":512},"obj":"CC"},{"id":"T309","span":{"begin":507,"end":508},"obj":"-COMMA-"},{"id":"T308","span":{"begin":500,"end":507},"obj":"NN"},{"id":"T307","span":{"begin":495,"end":499},"obj":"IN"},{"id":"T306","span":{"begin":487,"end":494},"obj":"VB"},{"id":"T305","span":{"begin":461,"end":486},"obj":"RB"},{"id":"T304","span":{"begin":456,"end":460},"obj":"VB"},{"id":"T303","span":{"begin":451,"end":455},"obj":"WDT"},{"id":"T302","span":{"begin":446,"end":450},"obj":"NN"},{"id":"T301","span":{"begin":443,"end":445},"obj":"IN"},{"id":"T300","span":{"begin":431,"end":442},"obj":"NN"},{"id":"T299","span":{"begin":428,"end":430},"obj":"IN"},{"id":"T298","span":{"begin":420,"end":427},"obj":"VB"},{"id":"T297","span":{"begin":416,"end":419},"obj":"VB"},{"id":"T296","span":{"begin":413,"end":415},"obj":"NN"},{"id":"T295","span":{"begin":405,"end":412},"obj":"NN"},{"id":"T294","span":{"begin":400,"end":402},"obj":"NN"},{"id":"T293","span":{"begin":380,"end":399},"obj":"JJ"},{"id":"T292","span":{"begin":377,"end":379},"obj":"IN"},{"id":"T291","span":{"begin":368,"end":376},"obj":"VB"},{"id":"T290","span":{"begin":361,"end":367},"obj":"NN"},{"id":"T289","span":{"begin":352,"end":360},"obj":"NN"},{"id":"T288","span":{"begin":348,"end":351},"obj":"NN"},{"id":"T287","span":{"begin":345,"end":347},"obj":"IN"},{"id":"T286","span":{"begin":338,"end":344},"obj":"NN"},{"id":"T285","span":{"begin":330,"end":337},"obj":"JJ"},{"id":"T284","span":{"begin":326,"end":329},"obj":"PRP-DOLLAR-"},{"id":"T283","span":{"begin":322,"end":325},"obj":"CC"},{"id":"T281","span":{"begin":306,"end":315},"obj":"JJ"},{"id":"T280","span":{"begin":299,"end":305},"obj":"IN"},{"id":"T279","span":{"begin":288,"end":298},"obj":"NN"},{"id":"T278","span":{"begin":286,"end":287},"obj":"-RRB-"},{"id":"T277","span":{"begin":283,"end":286},"obj":"NN"},{"id":"T276","span":{"begin":282,"end":283},"obj":"-LRB-"},{"id":"T275","span":{"begin":274,"end":281},"obj":"NN"},{"id":"T274","span":{"begin":262,"end":273},"obj":"NN"},{"id":"T273","span":{"begin":259,"end":261},"obj":"IN"},{"id":"T272","span":{"begin":256,"end":258},"obj":"NN"},{"id":"T271","span":{"begin":255,"end":256},"obj":"-RRB-"},{"id":"T270","span":{"begin":252,"end":255},"obj":"NN"},{"id":"T269","span":{"begin":251,"end":252},"obj":"-LRB-"},{"id":"T268","span":{"begin":244,"end":250},"obj":"NN"},{"id":"T267","span":{"begin":235,"end":243},"obj":"NN"},{"id":"T266","span":{"begin":229,"end":234},"obj":"NN"},{"id":"T265","span":{"begin":226,"end":228},"obj":"IN"},{"id":"T264","span":{"begin":220,"end":225},"obj":"NN"},{"id":"T263","span":{"begin":216,"end":219},"obj":"DT"},{"id":"T262","span":{"begin":203,"end":215},"obj":"VB"},{"id":"T261","span":{"begin":200,"end":202},"obj":"PRP"},{"id":"T260","span":{"begin":198,"end":199},"obj":"-COMMA-"},{"id":"T259","span":{"begin":193,"end":198},"obj":"NN"},{"id":"T258","span":{"begin":183,"end":192},"obj":"JJ"},{"id":"T257","span":{"begin":172,"end":182},"obj":"JJ"},{"id":"T256","span":{"begin":169,"end":171},"obj":"IN"},{"id":"T255","span":{"begin":159,"end":168},"obj":"NN"},{"id":"T254","span":{"begin":155,"end":158},"obj":"DT"},{"id":"T253","span":{"begin":145,"end":154},"obj":"VB"},{"id":"T252","span":{"begin":142,"end":144},"obj":"TO"},{"id":"T251","span":{"begin":133,"end":140},"obj":"NN"},{"id":"T250","span":{"begin":122,"end":132},"obj":"JJ"},{"id":"T249","span":{"begin":118,"end":121},"obj":"CC"},{"id":"T248","span":{"begin":109,"end":117},"obj":"JJ"},{"id":"T247","span":{"begin":106,"end":108},"obj":"IN"},{"id":"T246","span":{"begin":94,"end":105},"obj":"VB"},{"id":"T245","span":{"begin":92,"end":93},"obj":"-RRB-"},{"id":"T244","span":{"begin":90,"end":92},"obj":"NN"},{"id":"T243","span":{"begin":89,"end":90},"obj":"-LRB-"},{"id":"T242","span":{"begin":82,"end":88},"obj":"NN"},{"id":"T241","span":{"begin":73,"end":81},"obj":"NN"},{"id":"T240","span":{"begin":69,"end":72},"obj":"DT"},{"id":"T239","span":{"begin":66,"end":68},"obj":"IN"},{"id":"T238","span":{"begin":60,"end":65},"obj":"NN"},{"id":"T237","span":{"begin":50,"end":59},"obj":"JJ"},{"id":"T236","span":{"begin":43,"end":49},"obj":"JJ"},{"id":"T235","span":{"begin":35,"end":42},"obj":"VB"},{"id":"T234","span":{"begin":33,"end":34},"obj":"-RRB-"},{"id":"T233","span":{"begin":31,"end":33},"obj":"NN"},{"id":"T232","span":{"begin":30,"end":31},"obj":"-LRB-"},{"id":"T231","span":{"begin":18,"end":29},"obj":"NN"},{"id":"T230","span":{"begin":11,"end":17},"obj":"NN"},{"id":"T229","span":{"begin":0,"end":10},"obj":"NN"}],"relations":[{"id":"R62","pred":"arg1Of","subj":"T231","obj":"T230"},{"id":"R63","pred":"arg1Of","subj":"T231","obj":"T232"},{"id":"R64","pred":"arg1Of","subj":"T231","obj":"T235"},{"id":"R65","pred":"arg2Of","subj":"T233","obj":"T232"},{"id":"R66","pred":"arg3Of","subj":"T234","obj":"T232"},{"id":"R67","pred":"arg1Of","subj":"T235","obj":"T239"},{"id":"R68","pred":"arg2Of","subj":"T238","obj":"T235"},{"id":"R69","pred":"arg1Of","subj":"T238","obj":"T236"},{"id":"R70","pred":"arg1Of","subj":"T238","obj":"T237"},{"id":"R71","pred":"arg2Of","subj":"T242","obj":"T239"},{"id":"R72","pred":"arg1Of","subj":"T242","obj":"T240"},{"id":"R73","pred":"arg1Of","subj":"T242","obj":"T241"},{"id":"R74","pred":"arg1Of","subj":"T242","obj":"T243"},{"id":"R75","pred":"arg2Of","subj":"T242","obj":"T246"},{"id":"R76","pred":"arg2Of","subj":"T244","obj":"T243"},{"id":"R77","pred":"arg3Of","subj":"T245","obj":"T243"},{"id":"R78","pred":"arg1Of","subj":"T248","obj":"T249"},{"id":"R79","pred":"arg2Of","subj":"T250","obj":"T249"},{"id":"R80","pred":"arg1Of","subj":"T251","obj":"T246"},{"id":"R81","pred":"arg2Of","subj":"T251","obj":"T247"},{"id":"R82","pred":"arg1Of","subj":"T251","obj":"T248"},{"id":"R83","pred":"arg1Of","subj":"T251","obj":"T250"},{"id":"R84","pred":"arg1Of","subj":"T253","obj":"T252"},{"id":"R85","pred":"arg2Of","subj":"T255","obj":"T253"},{"id":"R86","pred":"arg1Of","subj":"T255","obj":"T254"},{"id":"R87","pred":"arg1Of","subj":"T255","obj":"T256"},{"id":"R88","pred":"arg2Of","subj":"T259","obj":"T256"},{"id":"R89","pred":"arg1Of","subj":"T259","obj":"T257"},{"id":"R90","pred":"arg1Of","subj":"T259","obj":"T258"},{"id":"R91","pred":"arg1Of","subj":"T261","obj":"T253"},{"id":"R92","pred":"arg1Of","subj":"T261","obj":"T262"},{"id":"R93","pred":"modOf","subj":"T262","obj":"T252"},{"id":"R94","pred":"arg1Of","subj":"T262","obj":"T260"},{"id":"R95","pred":"arg1Of","subj":"T262","obj":"T280"},{"id":"R96","pred":"arg1Of","subj":"T262","obj":"T287"},{"id":"R97","pred":"arg2Of","subj":"T264","obj":"T262"},{"id":"R98","pred":"arg1Of","subj":"T264","obj":"T263"},{"id":"R99","pred":"arg1Of","subj":"T264","obj":"T265"},{"id":"R100","pred":"arg1Of","subj":"T268","obj":"T266"},{"id":"R101","pred":"arg1Of","subj":"T268","obj":"T267"},{"id":"R102","pred":"arg1Of","subj":"T268","obj":"T269"},{"id":"R103","pred":"arg2Of","subj":"T270","obj":"T269"},{"id":"R104","pred":"arg3Of","subj":"T271","obj":"T269"},{"id":"R105","pred":"arg2Of","subj":"T272","obj":"T265"},{"id":"R106","pred":"arg1Of","subj":"T272","obj":"T268"},{"id":"R107","pred":"arg1Of","subj":"T272","obj":"T273"},{"id":"R108","pred":"arg1Of","subj":"T275","obj":"T274"},{"id":"R109","pred":"arg1Of","subj":"T275","obj":"T276"},{"id":"R110","pred":"arg2Of","subj":"T277","obj":"T276"},{"id":"R111","pred":"arg3Of","subj":"T278","obj":"T276"},{"id":"R112","pred":"arg2Of","subj":"T279","obj":"T273"},{"id":"R113","pred":"arg1Of","subj":"T279","obj":"T275"},{"id":"R114","pred":"arg1Of","subj":"T282","obj":"T281"},{"id":"R115","pred":"arg1Of","subj":"T282","obj":"T283"},{"id":"R116","pred":"arg2Of","subj":"T283","obj":"T280"},{"id":"R117","pred":"arg2Of","subj":"T286","obj":"T283"},{"id":"R118","pred":"arg1Of","subj":"T286","obj":"T284"},{"id":"R119","pred":"arg1Of","subj":"T286","obj":"T285"},{"id":"R120","pred":"arg2Of","subj":"T290","obj":"T287"},{"id":"R121","pred":"arg1Of","subj":"T290","obj":"T288"},{"id":"R122","pred":"arg1Of","subj":"T290","obj":"T289"},{"id":"R123","pred":"arg2Of","subj":"T290","obj":"T291"},{"id":"R124","pred":"arg1Of","subj":"T294","obj":"T291"},{"id":"R125","pred":"arg2Of","subj":"T294","obj":"T292"},{"id":"R126","pred":"arg1Of","subj":"T294","obj":"T293"},{"id":"R127","pred":"arg1Of","subj":"T296","obj":"T297"},{"id":"R128","pred":"arg2Of","subj":"T296","obj":"T298"},{"id":"R129","pred":"arg2Of","subj":"T298","obj":"T297"},{"id":"R130","pred":"arg1Of","subj":"T300","obj":"T301"},{"id":"R131","pred":"arg1Of","subj":"T300","obj":"T310"},{"id":"R132","pred":"arg2Of","subj":"T302","obj":"T301"},{"id":"R133","pred":"arg1Of","subj":"T302","obj":"T303"},{"id":"R134","pred":"arg1Of","subj":"T302","obj":"T304"},{"id":"R135","pred":"arg2Of","subj":"T302","obj":"T306"},{"id":"R136","pred":"arg2Of","subj":"T306","obj":"T304"},{"id":"R137","pred":"arg1Of","subj":"T306","obj":"T305"},{"id":"R138","pred":"arg1Of","subj":"T306","obj":"T307"},{"id":"R139","pred":"arg2Of","subj":"T308","obj":"T307"},{"id":"R140","pred":"arg1Of","subj":"T310","obj":"T298"},{"id":"R141","pred":"arg2Of","subj":"T310","obj":"T299"},{"id":"R142","pred":"arg1Of","subj":"T310","obj":"T309"},{"id":"R143","pred":"arg2Of","subj":"T314","obj":"T310"},{"id":"R144","pred":"arg1Of","subj":"T314","obj":"T311"},{"id":"R145","pred":"arg1Of","subj":"T314","obj":"T312"},{"id":"R146","pred":"arg1Of","subj":"T314","obj":"T313"},{"id":"R147","pred":"arg1Of","subj":"T314","obj":"T315"},{"id":"R148","pred":"arg1Of","subj":"T314","obj":"T318"},{"id":"R149","pred":"arg2Of","subj":"T316","obj":"T315"},{"id":"R150","pred":"arg3Of","subj":"T317","obj":"T315"},{"id":"R151","pred":"arg1Of","subj":"T318","obj":"T319"},{"id":"R152","pred":"arg2Of","subj":"T321","obj":"T319"},{"id":"R153","pred":"arg1Of","subj":"T321","obj":"T320"},{"id":"R154","pred":"arg1Of","subj":"T324","obj":"T325"},{"id":"R155","pred":"arg1Of","subj":"T325","obj":"T322"},{"id":"R156","pred":"arg1Of","subj":"T325","obj":"T323"},{"id":"R157","pred":"arg1Of","subj":"T328","obj":"T326"},{"id":"R158","pred":"arg1Of","subj":"T328","obj":"T327"},{"id":"R159","pred":"arg1Of","subj":"T328","obj":"T329"},{"id":"R160","pred":"arg2Of","subj":"T329","obj":"T325"},{"id":"R161","pred":"arg1Of","subj":"T329","obj":"T332"},{"id":"R162","pred":"arg2Of","subj":"T331","obj":"T329"},{"id":"R163","pred":"arg1Of","subj":"T331","obj":"T330"},{"id":"R164","pred":"arg1Of","subj":"T333","obj":"T334"},{"id":"R165","pred":"arg2Of","subj":"T335","obj":"T334"},{"id":"R166","pred":"arg2Of","subj":"T336","obj":"T332"},{"id":"R167","pred":"arg1Of","subj":"T336","obj":"T333"},{"id":"R168","pred":"arg1Of","subj":"T336","obj":"T335"},{"id":"R169","pred":"arg2Of","subj":"T339","obj":"T338"},{"id":"R170","pred":"arg1Of","subj":"T343","obj":"T341"},{"id":"R171","pred":"arg2Of","subj":"T343","obj":"T342"},{"id":"R172","pred":"arg1Of","subj":"T343","obj":"T344"},{"id":"R173","pred":"arg1Of","subj":"T344","obj":"T338"},{"id":"R174","pred":"arg1Of","subj":"T344","obj":"T340"},{"id":"R175","pred":"arg2Of","subj":"T347","obj":"T344"},{"id":"R176","pred":"arg1Of","subj":"T347","obj":"T345"},{"id":"R177","pred":"arg1Of","subj":"T347","obj":"T346"},{"id":"R178","pred":"arg2Of","subj":"T349","obj":"T348"},{"id":"R179","pred":"arg1Of","subj":"T354","obj":"T351"},{"id":"R180","pred":"arg1Of","subj":"T354","obj":"T352"},{"id":"R181","pred":"arg1Of","subj":"T354","obj":"T353"},{"id":"R182","pred":"arg1Of","subj":"T354","obj":"T355"},{"id":"R183","pred":"arg2Of","subj":"T354","obj":"T356"},{"id":"R184","pred":"arg1Of","subj":"T356","obj":"T348"},{"id":"R185","pred":"arg1Of","subj":"T356","obj":"T350"},{"id":"R186","pred":"arg2Of","subj":"T356","obj":"T355"},{"id":"R187","pred":"arg1Of","subj":"T356","obj":"T357"},{"id":"R188","pred":"arg1Of","subj":"T359","obj":"T358"},{"id":"R189","pred":"arg1Of","subj":"T359","obj":"T362"},{"id":"R190","pred":"arg2Of","subj":"T362","obj":"T357"},{"id":"R191","pred":"arg1Of","subj":"T362","obj":"T360"},{"id":"R192","pred":"arg1Of","subj":"T362","obj":"T361"},{"id":"R193","pred":"arg2Of","subj":"T363","obj":"T362"},{"id":"R194","pred":"arg2Of","subj":"T365","obj":"T364"},{"id":"R195","pred":"arg1Of","subj":"T369","obj":"T367"},{"id":"R196","pred":"arg1Of","subj":"T369","obj":"T368"},{"id":"R197","pred":"arg1Of","subj":"T369","obj":"T370"},{"id":"R198","pred":"arg2Of","subj":"T369","obj":"T371"},{"id":"R199","pred":"arg1Of","subj":"T371","obj":"T364"},{"id":"R200","pred":"arg1Of","subj":"T371","obj":"T366"},{"id":"R201","pred":"arg2Of","subj":"T371","obj":"T370"},{"id":"R202","pred":"arg1Of","subj":"T371","obj":"T372"},{"id":"R203","pred":"arg2Of","subj":"T374","obj":"T372"},{"id":"R204","pred":"arg1Of","subj":"T374","obj":"T373"},{"id":"R205","pred":"arg2Of","subj":"T374","obj":"T375"},{"id":"R206","pred":"arg1Of","subj":"T378","obj":"T375"},{"id":"R207","pred":"arg2Of","subj":"T378","obj":"T376"},{"id":"R208","pred":"arg1Of","subj":"T378","obj":"T377"},{"id":"R209","pred":"arg1Of","subj":"T378","obj":"T379"},{"id":"R210","pred":"arg2Of","subj":"T383","obj":"T379"},{"id":"R211","pred":"arg1Of","subj":"T383","obj":"T380"},{"id":"R212","pred":"arg1Of","subj":"T383","obj":"T381"},{"id":"R213","pred":"arg1Of","subj":"T383","obj":"T382"},{"id":"R214","pred":"arg3Of","subj":"T384","obj":"T379"},{"id":"R215","pred":"arg1Of","subj":"T385","obj":"T386"},{"id":"R216","pred":"arg1Of","subj":"T385","obj":"T388"},{"id":"R217","pred":"arg1Of","subj":"T385","obj":"T397"},{"id":"R218","pred":"arg2Of","subj":"T387","obj":"T386"},{"id":"R219","pred":"arg1Of","subj":"T392","obj":"T389"},{"id":"R220","pred":"arg1Of","subj":"T392","obj":"T390"},{"id":"R221","pred":"arg1Of","subj":"T392","obj":"T391"},{"id":"R222","pred":"arg1Of","subj":"T392","obj":"T393"},{"id":"R223","pred":"arg2Of","subj":"T394","obj":"T393"},{"id":"R224","pred":"arg3Of","subj":"T395","obj":"T393"},{"id":"R225","pred":"arg2Of","subj":"T396","obj":"T388"},{"id":"R226","pred":"arg1Of","subj":"T396","obj":"T392"},{"id":"R227","pred":"arg1Of","subj":"T397","obj":"T404"},{"id":"R228","pred":"arg1Of","subj":"T399","obj":"T400"},{"id":"R229","pred":"arg1Of","subj":"T399","obj":"T401"},{"id":"R230","pred":"arg2Of","subj":"T402","obj":"T401"},{"id":"R231","pred":"arg2Of","subj":"T403","obj":"T397"},{"id":"R232","pred":"arg1Of","subj":"T403","obj":"T398"},{"id":"R233","pred":"arg1Of","subj":"T403","obj":"T399"},{"id":"R234","pred":"arg1Of","subj":"T403","obj":"T402"},{"id":"R235","pred":"arg2Of","subj":"T405","obj":"T404"},{"id":"R236","pred":"arg1Of","subj":"T405","obj":"T406"},{"id":"R237","pred":"arg2Of","subj":"T408","obj":"T406"},{"id":"R238","pred":"arg1Of","subj":"T408","obj":"T407"},{"id":"R239","pred":"arg1Of","subj":"T408","obj":"T409"},{"id":"R240","pred":"arg2Of","subj":"T411","obj":"T409"},{"id":"R241","pred":"arg1Of","subj":"T411","obj":"T410"},{"id":"R242","pred":"arg1Of","subj":"T415","obj":"T414"},{"id":"R243","pred":"arg1Of","subj":"T415","obj":"T416"},{"id":"R244","pred":"arg1Of","subj":"T416","obj":"T412"},{"id":"R245","pred":"arg1Of","subj":"T416","obj":"T413"},{"id":"R246","pred":"arg2Of","subj":"T419","obj":"T416"},{"id":"R247","pred":"arg1Of","subj":"T419","obj":"T417"},{"id":"R248","pred":"arg1Of","subj":"T419","obj":"T418"},{"id":"R249","pred":"arg1Of","subj":"T419","obj":"T420"},{"id":"R250","pred":"arg2Of","subj":"T422","obj":"T420"},{"id":"R251","pred":"arg1Of","subj":"T422","obj":"T421"},{"id":"R252","pred":"arg1Of","subj":"T425","obj":"T424"},{"id":"R253","pred":"arg1Of","subj":"T425","obj":"T426"},{"id":"R254","pred":"arg1Of","subj":"T428","obj":"T429"},{"id":"R255","pred":"arg1Of","subj":"T428","obj":"T433"},{"id":"R256","pred":"arg1Of","subj":"T428","obj":"T439"},{"id":"R257","pred":"arg1Of","subj":"T428","obj":"T440"},{"id":"R258","pred":"arg2Of","subj":"T428","obj":"T441"},{"id":"R259","pred":"arg2Of","subj":"T432","obj":"T429"},{"id":"R260","pred":"arg1Of","subj":"T432","obj":"T430"},{"id":"R261","pred":"arg1Of","subj":"T432","obj":"T431"},{"id":"R262","pred":"arg2Of","subj":"T438","obj":"T433"},{"id":"R263","pred":"arg1Of","subj":"T438","obj":"T434"},{"id":"R264","pred":"arg1Of","subj":"T438","obj":"T435"},{"id":"R265","pred":"arg1Of","subj":"T438","obj":"T436"},{"id":"R266","pred":"arg1Of","subj":"T438","obj":"T437"},{"id":"R267","pred":"arg2Of","subj":"T441","obj":"T426"},{"id":"R268","pred":"arg1Of","subj":"T441","obj":"T427"},{"id":"R269","pred":"arg2Of","subj":"T441","obj":"T439"},{"id":"R270","pred":"arg2Of","subj":"T441","obj":"T440"},{"id":"R271","pred":"arg1Of","subj":"T441","obj":"T442"},{"id":"R272","pred":"arg2Of","subj":"T445","obj":"T442"},{"id":"R273","pred":"arg1Of","subj":"T445","obj":"T443"},{"id":"R274","pred":"arg1Of","subj":"T445","obj":"T444"},{"id":"R275","pred":"arg1Of","subj":"T449","obj":"T448"},{"id":"R276","pred":"arg1Of","subj":"T449","obj":"T450"},{"id":"R277","pred":"arg1Of","subj":"T450","obj":"T446"},{"id":"R278","pred":"arg1Of","subj":"T450","obj":"T447"},{"id":"R279","pred":"arg1Of","subj":"T450","obj":"T451"},{"id":"R280","pred":"arg1Of","subj":"T454","obj":"T452"},{"id":"R281","pred":"arg1Of","subj":"T454","obj":"T453"},{"id":"R282","pred":"arg1Of","subj":"T454","obj":"T455"},{"id":"R283","pred":"arg2Of","subj":"T455","obj":"T451"},{"id":"R284","pred":"arg2Of","subj":"T457","obj":"T455"},{"id":"R285","pred":"arg1Of","subj":"T457","obj":"T456"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T147","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T148","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T149","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T150","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T151","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T152","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T153","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T154","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T155","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T156","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T157","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T158","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T159","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T160","span":{"begin":1397,"end":1400},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T479","span":{"begin":1052,"end":1055},"obj":"http://www.uniprot.org/uniprot/P01589"},{"id":"T478","span":{"begin":525,"end":528},"obj":"http://www.uniprot.org/uniprot/P01589"},{"id":"T477","span":{"begin":1382,"end":1387},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T476","span":{"begin":1031,"end":1036},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T459","span":{"begin":884,"end":887},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T460","span":{"begin":1162,"end":1165},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T461","span":{"begin":1397,"end":1400},"obj":"http://www.uniprot.org/uniprot/Q04206"},{"id":"T463","span":{"begin":884,"end":887},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T464","span":{"begin":1162,"end":1165},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T465","span":{"begin":1397,"end":1400},"obj":"http://www.uniprot.org/uniprot/P21579"},{"id":"T466","span":{"begin":252,"end":255},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T467","span":{"begin":521,"end":524},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T468","span":{"begin":656,"end":659},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T469","span":{"begin":744,"end":747},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T470","span":{"begin":781,"end":784},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T471","span":{"begin":1031,"end":1034},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T472","span":{"begin":1048,"end":1051},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T473","span":{"begin":252,"end":258},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T474","span":{"begin":656,"end":661},"obj":"http://www.uniprot.org/uniprot/P01375"},{"id":"T475","span":{"begin":744,"end":749},"obj":"http://www.uniprot.org/uniprot/P01375"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"UBERON-AE","denotations":[{"id":"T95","span":{"begin":73,"end":88},"obj":"http://purl.obolibrary.org/obo/UBERON_0004725"},{"id":"T97","span":{"begin":82,"end":88},"obj":"http://purl.obolibrary.org/obo/UBERON_0001851"},{"id":"T98","span":{"begin":262,"end":267},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T99","span":{"begin":262,"end":281},"obj":"http://purl.obolibrary.org/obo/UBERON_0000120"},{"id":"T100","span":{"begin":268,"end":273},"obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"T101","span":{"begin":1519,"end":1528},"obj":"http://purl.obolibrary.org/obo/UBERON_2000106"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"GO-BP","denotations":[{"id":"T166","span":{"begin":235,"end":243},"obj":"http://purl.obolibrary.org/obo/GO_0019835"},{"id":"T173","span":{"begin":1409,"end":1430},"obj":"http://purl.obolibrary.org/obo/GO_0007252"},{"id":"T172","span":{"begin":1415,"end":1430},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T171","span":{"begin":1173,"end":1188},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T170","span":{"begin":895,"end":910},"obj":"http://purl.obolibrary.org/obo/GO_0016310"},{"id":"T168","span":{"begin":235,"end":243},"obj":"http://purl.obolibrary.org/obo/GO_0070265"},{"id":"T162","span":{"begin":235,"end":243},"obj":"http://purl.obolibrary.org/obo/GO_0001906"},{"id":"T164","span":{"begin":235,"end":243},"obj":"http://purl.obolibrary.org/obo/GO_0008219"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"GO-CC","denotations":[{"id":"T181","span":{"begin":837,"end":842},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T182","span":{"begin":940,"end":945},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T183","span":{"begin":1204,"end":1209},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T184","span":{"begin":1363,"end":1367},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"sentences","denotations":[{"id":"T117","span":{"begin":1478,"end":1582},"obj":"Sentence"},{"id":"T116","span":{"begin":1308,"end":1477},"obj":"Sentence"},{"id":"T115","span":{"begin":1297,"end":1307},"obj":"Sentence"},{"id":"T114","span":{"begin":1211,"end":1295},"obj":"Sentence"},{"id":"T113","span":{"begin":1013,"end":1210},"obj":"Sentence"},{"id":"T112","span":{"begin":866,"end":1012},"obj":"Sentence"},{"id":"T111","span":{"begin":768,"end":865},"obj":"Sentence"},{"id":"T110","span":{"begin":691,"end":767},"obj":"Sentence"},{"id":"T109","span":{"begin":683,"end":690},"obj":"Sentence"},{"id":"T108","span":{"begin":572,"end":681},"obj":"Sentence"},{"id":"T107","span":{"begin":413,"end":571},"obj":"Sentence"},{"id":"T106","span":{"begin":405,"end":412},"obj":"Sentence"},{"id":"T105","span":{"begin":142,"end":403},"obj":"Sentence"},{"id":"T104","span":{"begin":11,"end":141},"obj":"Sentence"},{"id":"T103","span":{"begin":0,"end":10},"obj":"Sentence"},{"id":"T3","span":{"begin":0,"end":10},"obj":"Sentence"},{"id":"T4","span":{"begin":11,"end":141},"obj":"Sentence"},{"id":"T5","span":{"begin":142,"end":403},"obj":"Sentence"},{"id":"T6","span":{"begin":405,"end":412},"obj":"Sentence"},{"id":"T7","span":{"begin":413,"end":571},"obj":"Sentence"},{"id":"T8","span":{"begin":572,"end":681},"obj":"Sentence"},{"id":"T9","span":{"begin":683,"end":690},"obj":"Sentence"},{"id":"T10","span":{"begin":691,"end":767},"obj":"Sentence"},{"id":"T11","span":{"begin":768,"end":865},"obj":"Sentence"},{"id":"T12","span":{"begin":866,"end":1012},"obj":"Sentence"},{"id":"T13","span":{"begin":1013,"end":1210},"obj":"Sentence"},{"id":"T14","span":{"begin":1211,"end":1295},"obj":"Sentence"},{"id":"T15","span":{"begin":1297,"end":1307},"obj":"Sentence"},{"id":"T16","span":{"begin":1308,"end":1477},"obj":"Sentence"},{"id":"T17","span":{"begin":1478,"end":1582},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"ICD10","denotations":[{"id":"T175","span":{"begin":11,"end":29},"obj":"http://purl.bioontology.org/ontology/ICD10/G41.9"},{"id":"T177","span":{"begin":11,"end":29},"obj":"http://purl.bioontology.org/ontology/ICD10/G41"},{"id":"T178","span":{"begin":352,"end":360},"obj":"http://purl.bioontology.org/ontology/ICD10/G40.9"},{"id":"T179","span":{"begin":352,"end":360},"obj":"http://purl.bioontology.org/ontology/ICD10/G40"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"simple1","denotations":[{"id":"T200","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T199","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T198","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T197","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T196","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T195","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T194","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T193","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T192","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T191","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T190","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T189","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T188","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T187","span":{"begin":229,"end":258},"obj":"Protein"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T911","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T912","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T913","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T914","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T915","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T916","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T917","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T918","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T919","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T920","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T921","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T922","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T923","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T924","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T926","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T927","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T928","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T929","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T930","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T931","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"}],"relations":[{"id":"R647","pred":"themeOf","subj":"T916","obj":"T926"},{"id":"R648","pred":"themeOf","subj":"T917","obj":"T928"},{"id":"R649","pred":"themeOf","subj":"T918","obj":"T931"},{"id":"R650","pred":"themeOf","subj":"T921","obj":"T929"},{"id":"R651","pred":"themeOf","subj":"T928","obj":"T927"},{"id":"R652","pred":"themeOf","subj":"T929","obj":"T930"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T768","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T765","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T764","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T763","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T762","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T761","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T760","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T759","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T758","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T757","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T756","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T755","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T754","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T753","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T775","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"},{"id":"T774","span":{"begin":1388,"end":1396},"obj":"Positive_regulation"},{"id":"T773","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T772","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T771","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"},{"id":"T770","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T769","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T752","span":{"begin":229,"end":258},"obj":"Protein"}],"relations":[{"id":"R556","pred":"themeOf","subj":"T757","obj":"T768"},{"id":"R557","pred":"themeOf","subj":"T758","obj":"T769"},{"id":"R558","pred":"themeOf","subj":"T759","obj":"T771"},{"id":"R559","pred":"themeOf","subj":"T762","obj":"T772"},{"id":"R560","pred":"causeOf","subj":"T764","obj":"T774"},{"id":"R561","pred":"themeOf","subj":"T765","obj":"T774"},{"id":"R562","pred":"themeOf","subj":"T765","obj":"T775"},{"id":"R564","pred":"themeOf","subj":"T769","obj":"T770"},{"id":"R565","pred":"themeOf","subj":"T772","obj":"T773"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"DLUT931","denotations":[{"id":"T819","span":{"begin":1388,"end":1396},"obj":"Positive_regulation"},{"id":"T818","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"},{"id":"T817","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T816","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T794","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T795","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T796","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T797","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T798","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T799","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T800","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T801","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T802","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T803","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T804","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T805","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T806","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T807","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T811","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T812","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T813","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T814","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"},{"id":"T815","span":{"begin":1076,"end":1084},"obj":"Positive_regulation"}],"relations":[{"id":"R566","pred":"themeOf","subj":"T816","obj":"T817"},{"id":"R567","pred":"themeOf","subj":"T818","obj":"T819"},{"id":"R625","pred":"themeOf","subj":"T799","obj":"T811"},{"id":"R626","pred":"themeOf","subj":"T800","obj":"T812"},{"id":"R627","pred":"themeOf","subj":"T801","obj":"T814"},{"id":"R628","pred":"themeOf","subj":"T802","obj":"T815"},{"id":"R629","pred":"themeOf","subj":"T804","obj":"T816"},{"id":"R630","pred":"causeOf","subj":"T806","obj":"T819"},{"id":"R631","pred":"themeOf","subj":"T807","obj":"T818"},{"id":"R634","pred":"themeOf","subj":"T812","obj":"T813"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T990","span":{"begin":1382,"end":1430},"obj":"Phosphorylation"},{"id":"T989","span":{"begin":413,"end":508},"obj":"Positive_regulation"},{"id":"T988","span":{"begin":413,"end":508},"obj":"Gene_expression"},{"id":"T986","span":{"begin":879,"end":924},"obj":"Positive_regulation"},{"id":"T985","span":{"begin":879,"end":910},"obj":"Phosphorylation"},{"id":"T984","span":{"begin":1382,"end":1430},"obj":"Regulation"},{"id":"T983","span":{"begin":781,"end":808},"obj":"Gene_expression"},{"id":"T982","span":{"begin":1351,"end":1367},"obj":"Entity"},{"id":"T981","span":{"begin":1224,"end":1232},"obj":"Entity"},{"id":"T980","span":{"begin":1003,"end":1010},"obj":"Entity"},{"id":"T979","span":{"begin":597,"end":608},"obj":"Entity"},{"id":"T978","span":{"begin":825,"end":864},"obj":"Entity"},{"id":"T977","span":{"begin":604,"end":608},"obj":"Protein"},{"id":"T976","span":{"begin":1382,"end":1430},"obj":"Entity"},{"id":"T975","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T974","span":{"begin":172,"end":182},"obj":"Entity"},{"id":"T973","span":{"begin":1409,"end":1414},"obj":"Protein"},{"id":"T972","span":{"begin":1028,"end":1036},"obj":"Protein"},{"id":"T971","span":{"begin":513,"end":548},"obj":"Protein"},{"id":"T970","span":{"begin":90,"end":92},"obj":"Protein"},{"id":"T969","span":{"begin":1233,"end":1241},"obj":"Entity"},{"id":"T968","span":{"begin":705,"end":729},"obj":"Entity"},{"id":"T967","span":{"begin":31,"end":33},"obj":"Entity"},{"id":"T966","span":{"begin":428,"end":442},"obj":"Entity"},{"id":"T965","span":{"begin":540,"end":543},"obj":"Protein"},{"id":"T964","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T963","span":{"begin":928,"end":978},"obj":"Entity"},{"id":"T936","span":{"begin":1159,"end":1172},"obj":"Entity"},{"id":"T932","span":{"begin":1037,"end":1075},"obj":"Protein"},{"id":"T933","span":{"begin":413,"end":415},"obj":"Protein"},{"id":"T934","span":{"begin":377,"end":402},"obj":"Entity"},{"id":"T937","span":{"begin":1529,"end":1539},"obj":"Entity"},{"id":"T939","span":{"begin":884,"end":894},"obj":"Entity"},{"id":"T941","span":{"begin":1261,"end":1271},"obj":"Entity"},{"id":"T942","span":{"begin":539,"end":547},"obj":"Entity"},{"id":"T943","span":{"begin":884,"end":894},"obj":"Protein"},{"id":"T944","span":{"begin":597,"end":608},"obj":"Entity"},{"id":"T945","span":{"begin":1450,"end":1460},"obj":"Entity"},{"id":"T946","span":{"begin":1382,"end":1430},"obj":"Protein"},{"id":"T947","span":{"begin":1096,"end":1098},"obj":"Entity"},{"id":"T948","span":{"begin":1159,"end":1172},"obj":"Protein"},{"id":"T949","span":{"begin":1288,"end":1294},"obj":"Protein"},{"id":"T950","span":{"begin":558,"end":560},"obj":"Entity"},{"id":"T951","span":{"begin":854,"end":864},"obj":"Entity"},{"id":"T952","span":{"begin":1076,"end":1084},"obj":"Entity"},{"id":"T953","span":{"begin":1224,"end":1232},"obj":"Protein"},{"id":"T954","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T955","span":{"begin":1250,"end":1260},"obj":"Entity"},{"id":"T956","span":{"begin":1382,"end":1396},"obj":"Protein"},{"id":"T957","span":{"begin":666,"end":671},"obj":"Protein"},{"id":"T958","span":{"begin":380,"end":399},"obj":"Entity"},{"id":"T959","span":{"begin":226,"end":258},"obj":"Protein"},{"id":"T960","span":{"begin":1192,"end":1209},"obj":"Entity"},{"id":"T961","span":{"begin":701,"end":704},"obj":"Protein"}],"relations":[{"id":"R600","pred":"themeOf","subj":"T964","obj":"T983"},{"id":"R601","pred":"causeOf","subj":"T966","obj":"T989"},{"id":"R602","pred":"themeOf","subj":"T973","obj":"T990"},{"id":"R603","pred":"partOf","subj":"T976","obj":"T973"},{"id":"R604","pred":"siteOf","subj":"T976","obj":"T990"},{"id":"R605","pred":"themeOf","subj":"T985","obj":"T986"},{"id":"R606","pred":"themeOf","subj":"T990","obj":"T984"},{"id":"R653","pred":"themeOf","subj":"T933","obj":"T988"},{"id":"R654","pred":"themeOf","subj":"T933","obj":"T989"},{"id":"R656","pred":"partOf","subj":"T939","obj":"T943"},{"id":"R657","pred":"siteOf","subj":"T939","obj":"T985"},{"id":"R658","pred":"themeOf","subj":"T943","obj":"T985"},{"id":"R659","pred":"causeOf","subj":"T956","obj":"T984"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T575","span":{"begin":400,"end":402},"obj":"NNP"},{"id":"T574","span":{"begin":380,"end":399},"obj":"JJ"},{"id":"T573","span":{"begin":377,"end":379},"obj":"IN"},{"id":"T572","span":{"begin":368,"end":376},"obj":"VBN"},{"id":"T571","span":{"begin":361,"end":367},"obj":"NNS"},{"id":"T570","span":{"begin":352,"end":360},"obj":"NN"},{"id":"T569","span":{"begin":348,"end":351},"obj":"NN"},{"id":"T568","span":{"begin":345,"end":347},"obj":"IN"},{"id":"T567","span":{"begin":338,"end":344},"obj":"NNS"},{"id":"T566","span":{"begin":330,"end":337},"obj":"JJ"},{"id":"T565","span":{"begin":326,"end":329},"obj":"PRP$"},{"id":"T564","span":{"begin":322,"end":325},"obj":"CC"},{"id":"T563","span":{"begin":316,"end":321},"obj":"NN"},{"id":"T562","span":{"begin":306,"end":315},"obj":"JJ"},{"id":"T561","span":{"begin":299,"end":305},"obj":"IN"},{"id":"T560","span":{"begin":288,"end":298},"obj":"NN"},{"id":"T559","span":{"begin":286,"end":287},"obj":"-RRB-"},{"id":"T558","span":{"begin":283,"end":286},"obj":"NNP"},{"id":"T557","span":{"begin":282,"end":283},"obj":"-LRB-"},{"id":"T556","span":{"begin":274,"end":281},"obj":"NN"},{"id":"T555","span":{"begin":262,"end":273},"obj":"JJ"},{"id":"T554","span":{"begin":259,"end":261},"obj":"IN"},{"id":"T553","span":{"begin":257,"end":258},"obj":"NN"},{"id":"T552","span":{"begin":256,"end":257},"obj":":"},{"id":"T551","span":{"begin":255,"end":256},"obj":"-RRB-"},{"id":"T550","span":{"begin":252,"end":255},"obj":"NNP"},{"id":"T549","span":{"begin":251,"end":252},"obj":"-LRB-"},{"id":"T548","span":{"begin":244,"end":250},"obj":"NN"},{"id":"T547","span":{"begin":235,"end":243},"obj":"NN"},{"id":"T546","span":{"begin":229,"end":234},"obj":"NN"},{"id":"T545","span":{"begin":226,"end":228},"obj":"IN"},{"id":"T544","span":{"begin":220,"end":225},"obj":"NNS"},{"id":"T543","span":{"begin":216,"end":219},"obj":"DT"},{"id":"T542","span":{"begin":203,"end":215},"obj":"VBD"},{"id":"T541","span":{"begin":200,"end":202},"obj":"PRP"},{"id":"T540","span":{"begin":198,"end":199},"obj":","},{"id":"T539","span":{"begin":193,"end":198},"obj":"NN"},{"id":"T538","span":{"begin":183,"end":192},"obj":"JJ"},{"id":"T537","span":{"begin":172,"end":182},"obj":"JJ"},{"id":"T536","span":{"begin":169,"end":171},"obj":"IN"},{"id":"T535","span":{"begin":159,"end":168},"obj":"NN"},{"id":"T534","span":{"begin":155,"end":158},"obj":"DT"},{"id":"T533","span":{"begin":145,"end":154},"obj":"VB"},{"id":"T532","span":{"begin":142,"end":144},"obj":"TO"},{"id":"T531","span":{"begin":140,"end":141},"obj":"."},{"id":"T530","span":{"begin":133,"end":140},"obj":"NNS"},{"id":"T529","span":{"begin":122,"end":132},"obj":"JJ"},{"id":"T528","span":{"begin":118,"end":121},"obj":"CC"},{"id":"T527","span":{"begin":109,"end":117},"obj":"JJ"},{"id":"T526","span":{"begin":106,"end":108},"obj":"IN"},{"id":"T525","span":{"begin":94,"end":105},"obj":"VBN"},{"id":"T524","span":{"begin":92,"end":93},"obj":"-RRB-"},{"id":"T523","span":{"begin":90,"end":92},"obj":"NN"},{"id":"T522","span":{"begin":89,"end":90},"obj":"-LRB-"},{"id":"T521","span":{"begin":82,"end":88},"obj":"NN"},{"id":"T520","span":{"begin":73,"end":81},"obj":"NN"},{"id":"T519","span":{"begin":69,"end":72},"obj":"DT"},{"id":"T518","span":{"begin":66,"end":68},"obj":"IN"},{"id":"T517","span":{"begin":60,"end":65},"obj":"NN"},{"id":"T516","span":{"begin":50,"end":59},"obj":"JJ"},{"id":"T515","span":{"begin":43,"end":49},"obj":"JJ"},{"id":"T514","span":{"begin":35,"end":42},"obj":"VBZ"},{"id":"T513","span":{"begin":33,"end":34},"obj":"-RRB-"},{"id":"T512","span":{"begin":31,"end":33},"obj":"FW"},{"id":"T511","span":{"begin":30,"end":31},"obj":"-LRB-"},{"id":"T510","span":{"begin":18,"end":29},"obj":"NN"},{"id":"T509","span":{"begin":11,"end":17},"obj":"NNP"},{"id":"T508","span":{"begin":0,"end":10},"obj":"NNP"},{"id":"T749","span":{"begin":1581,"end":1582},"obj":"."},{"id":"T748","span":{"begin":1577,"end":1581},"obj":"NN"},{"id":"T747","span":{"begin":1557,"end":1576},"obj":"JJ"},{"id":"T746","span":{"begin":1553,"end":1556},"obj":"CC"},{"id":"T745","span":{"begin":1540,"end":1552},"obj":"NN"},{"id":"T744","span":{"begin":1529,"end":1539},"obj":"NN"},{"id":"T743","span":{"begin":1519,"end":1528},"obj":"JJ"},{"id":"T742","span":{"begin":1516,"end":1518},"obj":"IN"},{"id":"T741","span":{"begin":1508,"end":1515},"obj":"NNS"},{"id":"T740","span":{"begin":1502,"end":1507},"obj":"NN"},{"id":"T739","span":{"begin":1492,"end":1501},"obj":"JJ"},{"id":"T738","span":{"begin":1490,"end":1491},"obj":","},{"id":"T737","span":{"begin":1478,"end":1490},"obj":"RB"},{"id":"T736","span":{"begin":1476,"end":1477},"obj":"."},{"id":"T735","span":{"begin":1471,"end":1476},"obj":"NN"},{"id":"T734","span":{"begin":1461,"end":1470},"obj":"JJ"},{"id":"T733","span":{"begin":1450,"end":1460},"obj":"JJ"},{"id":"T732","span":{"begin":1447,"end":1449},"obj":"IN"},{"id":"T731","span":{"begin":1438,"end":1446},"obj":"VBN"},{"id":"T730","span":{"begin":1435,"end":1437},"obj":"VB"},{"id":"T729","span":{"begin":1431,"end":1434},"obj":"MD"},{"id":"T728","span":{"begin":1415,"end":1430},"obj":"NN"},{"id":"T727","span":{"begin":1409,"end":1414},"obj":"NN"},{"id":"T726","span":{"begin":1405,"end":1408},"obj":"CD"},{"id":"T725","span":{"begin":1397,"end":1404},"obj":"NN"},{"id":"T724","span":{"begin":1382,"end":1396},"obj":"JJ"},{"id":"T723","span":{"begin":1378,"end":1381},"obj":"IN"},{"id":"T722","span":{"begin":1368,"end":1377},"obj":"NNS"},{"id":"T721","span":{"begin":1363,"end":1367},"obj":"NN"},{"id":"T720","span":{"begin":1351,"end":1362},"obj":"JJ"},{"id":"T719","span":{"begin":1348,"end":1350},"obj":"IN"},{"id":"T718","span":{"begin":1336,"end":1347},"obj":"NNS"},{"id":"T717","span":{"begin":1331,"end":1335},"obj":"IN"},{"id":"T716","span":{"begin":1323,"end":1330},"obj":"VBP"},{"id":"T715","span":{"begin":1314,"end":1322},"obj":"NNS"},{"id":"T714","span":{"begin":1308,"end":1313},"obj":"DT"},{"id":"T713","span":{"begin":1297,"end":1307},"obj":"NNP"},{"id":"T712","span":{"begin":1294,"end":1295},"obj":"."},{"id":"T711","span":{"begin":1292,"end":1294},"obj":"NN"},{"id":"T710","span":{"begin":1288,"end":1291},"obj":"DT"},{"id":"T709","span":{"begin":1285,"end":1287},"obj":"IN"},{"id":"T708","span":{"begin":1272,"end":1284},"obj":"NN"},{"id":"T707","span":{"begin":1261,"end":1271},"obj":"NN"},{"id":"T706","span":{"begin":1250,"end":1260},"obj":"JJ"},{"id":"T705","span":{"begin":1242,"end":1249},"obj":"VBN"},{"id":"T704","span":{"begin":1233,"end":1241},"obj":"NN"},{"id":"T703","span":{"begin":1224,"end":1232},"obj":"JJ"},{"id":"T702","span":{"begin":1222,"end":1223},"obj":","},{"id":"T701","span":{"begin":1211,"end":1222},"obj":"RB"},{"id":"T700","span":{"begin":1209,"end":1210},"obj":"."},{"id":"T699","span":{"begin":1204,"end":1209},"obj":"NNS"},{"id":"T698","span":{"begin":1192,"end":1203},"obj":"JJ"},{"id":"T697","span":{"begin":1189,"end":1191},"obj":"IN"},{"id":"T696","span":{"begin":1173,"end":1188},"obj":"NN"},{"id":"T695","span":{"begin":1162,"end":1172},"obj":"NN"},{"id":"T694","span":{"begin":1159,"end":1161},"obj":"IN"},{"id":"T693","span":{"begin":1148,"end":1158},"obj":"NN"},{"id":"T692","span":{"begin":1144,"end":1147},"obj":"IN"},{"id":"T691","span":{"begin":1136,"end":1143},"obj":"NNS"},{"id":"T690","span":{"begin":1127,"end":1135},"obj":"JJ"},{"id":"T689","span":{"begin":1123,"end":1126},"obj":"CC"},{"id":"T688","span":{"begin":1117,"end":1122},"obj":"NN"},{"id":"T687","span":{"begin":1107,"end":1116},"obj":"JJ"},{"id":"T686","span":{"begin":1096,"end":1106},"obj":"JJ"},{"id":"T685","span":{"begin":1085,"end":1095},"obj":"VBD"},{"id":"T684","span":{"begin":1076,"end":1084},"obj":"NN"},{"id":"T683","span":{"begin":1074,"end":1075},"obj":"-RRB-"},{"id":"T682","span":{"begin":1066,"end":1074},"obj":"JJ"},{"id":"T681","span":{"begin":1065,"end":1066},"obj":"-LRB-"},{"id":"T680","span":{"begin":1056,"end":1064},"obj":"NN"},{"id":"T679","span":{"begin":1052,"end":1055},"obj":"NN"},{"id":"T678","span":{"begin":1048,"end":1051},"obj":"NNP"},{"id":"T677","span":{"begin":1040,"end":1047},"obj":"JJ"},{"id":"T676","span":{"begin":1037,"end":1039},"obj":"IN"},{"id":"T675","span":{"begin":1031,"end":1036},"obj":"JJ"},{"id":"T674","span":{"begin":1028,"end":1030},"obj":"IN"},{"id":"T673","span":{"begin":1013,"end":1027},"obj":"NN"},{"id":"T672","span":{"begin":1011,"end":1012},"obj":"."},{"id":"T671","span":{"begin":1010,"end":1011},"obj":"-RRB-"},{"id":"T670","span":{"begin":1003,"end":1010},"obj":"NN"},{"id":"T669","span":{"begin":995,"end":1002},"obj":"NN"},{"id":"T668","span":{"begin":983,"end":994},"obj":"JJ"},{"id":"T667","span":{"begin":980,"end":982},"obj":"DT"},{"id":"T666","span":{"begin":979,"end":980},"obj":"-LRB-"},{"id":"T665","span":{"begin":968,"end":978},"obj":"NN"},{"id":"T664","span":{"begin":961,"end":967},"obj":"NNP"},{"id":"T663","span":{"begin":958,"end":960},"obj":"IN"},{"id":"T662","span":{"begin":946,"end":957},"obj":"VBN"},{"id":"T661","span":{"begin":940,"end":945},"obj":"NNS"},{"id":"T660","span":{"begin":928,"end":939},"obj":"JJ"},{"id":"T659","span":{"begin":925,"end":927},"obj":"IN"},{"id":"T658","span":{"begin":915,"end":924},"obj":"VBN"},{"id":"T657","span":{"begin":911,"end":914},"obj":"VBD"},{"id":"T656","span":{"begin":895,"end":910},"obj":"NN"},{"id":"T655","span":{"begin":884,"end":894},"obj":"JJ"},{"id":"T654","span":{"begin":879,"end":883},"obj":"RB"},{"id":"T653","span":{"begin":877,"end":878},"obj":","},{"id":"T652","span":{"begin":869,"end":877},"obj":"NN"},{"id":"T651","span":{"begin":866,"end":868},"obj":"IN"},{"id":"T650","span":{"begin":864,"end":865},"obj":"."},{"id":"T649","span":{"begin":854,"end":864},"obj":"NNS"},{"id":"T648","span":{"begin":851,"end":853},"obj":"IN"},{"id":"T647","span":{"begin":846,"end":850},"obj":"RB"},{"id":"T646","span":{"begin":843,"end":845},"obj":"RB"},{"id":"T645","span":{"begin":837,"end":842},"obj":"NNS"},{"id":"T644","span":{"begin":825,"end":836},"obj":"JJ"},{"id":"T643","span":{"begin":822,"end":824},"obj":"IN"},{"id":"T642","span":{"begin":813,"end":821},"obj":"VBN"},{"id":"T641","span":{"begin":809,"end":812},"obj":"VBD"},{"id":"T640","span":{"begin":798,"end":808},"obj":"NN"},{"id":"T639","span":{"begin":789,"end":797},"obj":"NN"},{"id":"T638","span":{"begin":785,"end":788},"obj":"NN"},{"id":"T637","span":{"begin":781,"end":784},"obj":"NNP"},{"id":"T636","span":{"begin":779,"end":780},"obj":","},{"id":"T635","span":{"begin":771,"end":779},"obj":"NN"},{"id":"T634","span":{"begin":768,"end":770},"obj":"IN"},{"id":"T633","span":{"begin":766,"end":767},"obj":"."},{"id":"T632","span":{"begin":750,"end":766},"obj":"NN"},{"id":"T631","span":{"begin":744,"end":749},"obj":"JJ"},{"id":"T630","span":{"begin":737,"end":743},"obj":"JJ"},{"id":"T629","span":{"begin":730,"end":736},"obj":"VBD"},{"id":"T628","span":{"begin":720,"end":729},"obj":"NN"},{"id":"T627","span":{"begin":710,"end":719},"obj":"VBN"},{"id":"T626","span":{"begin":705,"end":709},"obj":"JJS"},{"id":"T625","span":{"begin":703,"end":704},"obj":","},{"id":"T624","span":{"begin":701,"end":703},"obj":"NNP"},{"id":"T623","span":{"begin":691,"end":700},"obj":"VBG"},{"id":"T622","span":{"begin":683,"end":690},"obj":"NNS"},{"id":"T621","span":{"begin":680,"end":681},"obj":"."},{"id":"T620","span":{"begin":672,"end":680},"obj":"NNS"},{"id":"T619","span":{"begin":666,"end":671},"obj":"NNP"},{"id":"T618","span":{"begin":662,"end":665},"obj":"CC"},{"id":"T617","span":{"begin":656,"end":661},"obj":"NNP"},{"id":"T616","span":{"begin":652,"end":655},"obj":"IN"},{"id":"T615","span":{"begin":644,"end":651},"obj":"NNS"},{"id":"T614","span":{"begin":624,"end":643},"obj":"JJ"},{"id":"T613","span":{"begin":620,"end":623},"obj":"CC"},{"id":"T612","span":{"begin":611,"end":619},"obj":"VBG"},{"id":"T611","span":{"begin":609,"end":610},"obj":"NNP"},{"id":"T610","span":{"begin":597,"end":608},"obj":"NNP"},{"id":"T609","span":{"begin":587,"end":596},"obj":"VBD"},{"id":"T608","span":{"begin":584,"end":586},"obj":"PRP"},{"id":"T607","span":{"begin":582,"end":583},"obj":","},{"id":"T606","span":{"begin":572,"end":582},"obj":"RB"},{"id":"T605","span":{"begin":570,"end":571},"obj":"."},{"id":"T604","span":{"begin":561,"end":570},"obj":"NN"},{"id":"T603","span":{"begin":558,"end":560},"obj":"VB"},{"id":"T602","span":{"begin":555,"end":557},"obj":"TO"},{"id":"T601","span":{"begin":549,"end":554},"obj":"RB"},{"id":"T600","span":{"begin":547,"end":548},"obj":"-RRB-"},{"id":"T599","span":{"begin":539,"end":547},"obj":"JJ"},{"id":"T598","span":{"begin":538,"end":539},"obj":"-LRB-"},{"id":"T597","span":{"begin":529,"end":537},"obj":"NN"},{"id":"T596","span":{"begin":525,"end":528},"obj":"NN"},{"id":"T595","span":{"begin":521,"end":524},"obj":"NNP"},{"id":"T594","span":{"begin":513,"end":520},"obj":"JJ"},{"id":"T593","span":{"begin":509,"end":512},"obj":"CC"},{"id":"T592","span":{"begin":507,"end":508},"obj":","},{"id":"T591","span":{"begin":506,"end":507},"obj":":"},{"id":"T590","span":{"begin":500,"end":506},"obj":"NN"},{"id":"T589","span":{"begin":495,"end":499},"obj":"IN"},{"id":"T588","span":{"begin":487,"end":494},"obj":"VBN"},{"id":"T587","span":{"begin":461,"end":486},"obj":"RB"},{"id":"T586","span":{"begin":456,"end":460},"obj":"VBD"},{"id":"T585","span":{"begin":451,"end":455},"obj":"WDT"},{"id":"T584","span":{"begin":446,"end":450},"obj":"NNS"},{"id":"T583","span":{"begin":443,"end":445},"obj":"IN"},{"id":"T582","span":{"begin":431,"end":442},"obj":"NN"},{"id":"T581","span":{"begin":428,"end":430},"obj":"IN"},{"id":"T580","span":{"begin":420,"end":427},"obj":"VBN"},{"id":"T579","span":{"begin":416,"end":419},"obj":"VBD"},{"id":"T578","span":{"begin":413,"end":415},"obj":"NNP"},{"id":"T577","span":{"begin":405,"end":412},"obj":"NNP"},{"id":"T576","span":{"begin":402,"end":403},"obj":"."}],"relations":[{"id":"R314","pred":"compound","subj":"T508","obj":"T509"},{"id":"R315","pred":"compound","subj":"T509","obj":"T510"},{"id":"R317","pred":"punct","subj":"T511","obj":"T512"},{"id":"R318","pred":"appos","subj":"T512","obj":"T510"},{"id":"R319","pred":"punct","subj":"T513","obj":"T510"},{"id":"R321","pred":"amod","subj":"T515","obj":"T517"},{"id":"R322","pred":"amod","subj":"T516","obj":"T517"},{"id":"R323","pred":"dobj","subj":"T517","obj":"T514"},{"id":"R324","pred":"prep","subj":"T518","obj":"T514"},{"id":"R325","pred":"det","subj":"T519","obj":"T521"},{"id":"R326","pred":"compound","subj":"T520","obj":"T521"},{"id":"R327","pred":"pobj","subj":"T521","obj":"T518"},{"id":"R328","pred":"punct","subj":"T522","obj":"T521"},{"id":"R329","pred":"appos","subj":"T523","obj":"T521"},{"id":"R330","pred":"punct","subj":"T524","obj":"T521"},{"id":"R331","pred":"advcl","subj":"T525","obj":"T514"},{"id":"R332","pred":"agent","subj":"T526","obj":"T525"},{"id":"R333","pred":"amod","subj":"T527","obj":"T530"},{"id":"R334","pred":"cc","subj":"T528","obj":"T527"},{"id":"R335","pred":"conj","subj":"T529","obj":"T527"},{"id":"R336","pred":"pobj","subj":"T530","obj":"T526"},{"id":"R338","pred":"aux","subj":"T532","obj":"T533"},{"id":"R339","pred":"advcl","subj":"T533","obj":"T542"},{"id":"R340","pred":"det","subj":"T534","obj":"T535"},{"id":"R341","pred":"dobj","subj":"T535","obj":"T533"},{"id":"R342","pred":"prep","subj":"T536","obj":"T535"},{"id":"R343","pred":"amod","subj":"T537","obj":"T539"},{"id":"R344","pred":"amod","subj":"T538","obj":"T539"},{"id":"R345","pred":"pobj","subj":"T539","obj":"T536"},{"id":"R346","pred":"punct","subj":"T540","obj":"T542"},{"id":"R347","pred":"nsubj","subj":"T541","obj":"T542"},{"id":"R348","pred":"ROOT","subj":"T542","obj":"T542"},{"id":"R349","pred":"det","subj":"T543","obj":"T544"},{"id":"R350","pred":"dobj","subj":"T544","obj":"T542"},{"id":"R351","pred":"prep","subj":"T545","obj":"T544"},{"id":"R352","pred":"compound","subj":"T546","obj":"T547"},{"id":"R353","pred":"compound","subj":"T547","obj":"T548"},{"id":"R354","pred":"pobj","subj":"T548","obj":"T545"},{"id":"R355","pred":"punct","subj":"T549","obj":"T550"},{"id":"R356","pred":"appos","subj":"T550","obj":"T548"},{"id":"R357","pred":"punct","subj":"T551","obj":"T550"},{"id":"R358","pred":"punct","subj":"T552","obj":"T553"},{"id":"R359","pred":"conj","subj":"T553","obj":"T544"},{"id":"R360","pred":"prep","subj":"T554","obj":"T553"},{"id":"R361","pred":"amod","subj":"T555","obj":"T556"},{"id":"R362","pred":"pobj","subj":"T556","obj":"T554"},{"id":"R363","pred":"punct","subj":"T557","obj":"T558"},{"id":"R364","pred":"appos","subj":"T558","obj":"T553"},{"id":"R365","pred":"punct","subj":"T559","obj":"T553"},{"id":"R366","pred":"npadvmod","subj":"T560","obj":"T542"},{"id":"R367","pred":"prep","subj":"T561","obj":"T560"},{"id":"R368","pred":"amod","subj":"T562","obj":"T563"},{"id":"R369","pred":"pobj","subj":"T563","obj":"T561"},{"id":"R370","pred":"cc","subj":"T564","obj":"T563"},{"id":"R371","pred":"poss","subj":"T565","obj":"T567"},{"id":"R372","pred":"amod","subj":"T566","obj":"T567"},{"id":"R373","pred":"conj","subj":"T567","obj":"T563"},{"id":"R374","pred":"prep","subj":"T568","obj":"T567"},{"id":"R375","pred":"compound","subj":"T569","obj":"T571"},{"id":"R376","pred":"compound","subj":"T570","obj":"T571"},{"id":"R377","pred":"pobj","subj":"T571","obj":"T568"},{"id":"R378","pred":"acl","subj":"T572","obj":"T571"},{"id":"R379","pred":"agent","subj":"T573","obj":"T572"},{"id":"R380","pred":"amod","subj":"T574","obj":"T575"},{"id":"R381","pred":"pobj","subj":"T575","obj":"T573"},{"id":"R382","pred":"punct","subj":"T576","obj":"T542"},{"id":"R383","pred":"compound","subj":"T577","obj":"T578"},{"id":"R384","pred":"nsubjpass","subj":"T578","obj":"T580"},{"id":"R385","pred":"auxpass","subj":"T579","obj":"T580"},{"id":"R386","pred":"ROOT","subj":"T580","obj":"T580"},{"id":"R387","pred":"agent","subj":"T581","obj":"T580"},{"id":"R388","pred":"pobj","subj":"T582","obj":"T581"},{"id":"R389","pred":"prep","subj":"T583","obj":"T580"},{"id":"R390","pred":"pobj","subj":"T584","obj":"T583"},{"id":"R391","pred":"nsubjpass","subj":"T585","obj":"T588"},{"id":"R392","pred":"auxpass","subj":"T586","obj":"T588"},{"id":"R393","pred":"advmod","subj":"T587","obj":"T588"},{"id":"R394","pred":"relcl","subj":"T588","obj":"T584"},{"id":"R395","pred":"prep","subj":"T589","obj":"T588"},{"id":"R396","pred":"pobj","subj":"T590","obj":"T589"},{"id":"R397","pred":"punct","subj":"T591","obj":"T590"},{"id":"R398","pred":"punct","subj":"T592","obj":"T590"},{"id":"R399","pred":"cc","subj":"T593","obj":"T590"},{"id":"R400","pred":"conj","subj":"T594","obj":"T590"},{"id":"R401","pred":"nmod","subj":"T595","obj":"T597"},{"id":"R402","pred":"compound","subj":"T596","obj":"T597"},{"id":"R403","pred":"conj","subj":"T597","obj":"T590"},{"id":"R404","pred":"punct","subj":"T598","obj":"T597"},{"id":"R405","pred":"appos","subj":"T599","obj":"T597"},{"id":"R406","pred":"punct","subj":"T600","obj":"T597"},{"id":"R407","pred":"advmod","subj":"T601","obj":"T588"},{"id":"R408","pred":"prep","subj":"T602","obj":"T601"},{"id":"R409","pred":"compound","subj":"T603","obj":"T604"},{"id":"R410","pred":"pobj","subj":"T604","obj":"T602"},{"id":"R411","pred":"punct","subj":"T605","obj":"T580"},{"id":"R412","pred":"advmod","subj":"T606","obj":"T609"},{"id":"R413","pred":"punct","subj":"T607","obj":"T609"},{"id":"R414","pred":"nsubj","subj":"T608","obj":"T609"},{"id":"R415","pred":"ROOT","subj":"T609","obj":"T609"},{"id":"R416","pred":"compound","subj":"T610","obj":"T611"},{"id":"R453","pred":"advmod","subj":"T647","obj":"T648"},{"id":"R454","pred":"cc","subj":"T648","obj":"T645"},{"id":"R455","pred":"conj","subj":"T649","obj":"T645"},{"id":"R456","pred":"punct","subj":"T650","obj":"T642"},{"id":"R457","pred":"prep","subj":"T651","obj":"T658"},{"id":"R458","pred":"pobj","subj":"T652","obj":"T651"},{"id":"R459","pred":"punct","subj":"T653","obj":"T658"},{"id":"R510","pred":"nsubj","subj":"T704","obj":"T705"},{"id":"R511","pred":"ROOT","subj":"T705","obj":"T705"},{"id":"R512","pred":"amod","subj":"T706","obj":"T708"},{"id":"R513","pred":"compound","subj":"T707","obj":"T708"},{"id":"R514","pred":"dobj","subj":"T708","obj":"T705"},{"id":"R515","pred":"prep","subj":"T709","obj":"T705"},{"id":"R516","pred":"det","subj":"T710","obj":"T711"},{"id":"R517","pred":"pobj","subj":"T711","obj":"T709"},{"id":"R528","pred":"pobj","subj":"T722","obj":"T719"},{"id":"R529","pred":"prep","subj":"T723","obj":"T718"},{"id":"R530","pred":"amod","subj":"T724","obj":"T728"},{"id":"R531","pred":"nmod","subj":"T725","obj":"T728"},{"id":"R553","pred":"amod","subj":"T747","obj":"T748"},{"id":"R554","pred":"conj","subj":"T748","obj":"T745"},{"id":"R555","pred":"punct","subj":"T749","obj":"T741"},{"id":"R532","pred":"nummod","subj":"T726","obj":"T728"},{"id":"R533","pred":"compound","subj":"T727","obj":"T728"},{"id":"R534","pred":"pobj","subj":"T728","obj":"T723"},{"id":"R535","pred":"aux","subj":"T729","obj":"T731"},{"id":"R536","pred":"auxpass","subj":"T730","obj":"T731"},{"id":"R537","pred":"ccomp","subj":"T731","obj":"T716"},{"id":"R538","pred":"prep","subj":"T732","obj":"T731"},{"id":"R539","pred":"amod","subj":"T733","obj":"T735"},{"id":"R540","pred":"amod","subj":"T734","obj":"T735"},{"id":"R541","pred":"pobj","subj":"T735","obj":"T732"},{"id":"R542","pred":"punct","subj":"T736","obj":"T716"},{"id":"R543","pred":"advmod","subj":"T737","obj":"T741"},{"id":"R544","pred":"punct","subj":"T738","obj":"T741"},{"id":"R545","pred":"amod","subj":"T739","obj":"T741"},{"id":"R546","pred":"compound","subj":"T740","obj":"T741"},{"id":"R547","pred":"ROOT","subj":"T741","obj":"T741"},{"id":"R548","pred":"prep","subj":"T742","obj":"T741"},{"id":"R549","pred":"amod","subj":"T743","obj":"T745"},{"id":"R550","pred":"compound","subj":"T744","obj":"T745"},{"id":"R551","pred":"pobj","subj":"T745","obj":"T742"},{"id":"R552","pred":"cc","subj":"T746","obj":"T745"},{"id":"R417","pred":"dobj","subj":"T611","obj":"T609"},{"id":"R418","pred":"prep","subj":"T612","obj":"T609"},{"id":"R419","pred":"cc","subj":"T613","obj":"T612"},{"id":"R420","pred":"amod","subj":"T614","obj":"T615"},{"id":"R421","pred":"conj","subj":"T615","obj":"T612"},{"id":"R422","pred":"prep","subj":"T616","obj":"T615"},{"id":"R423","pred":"nmod","subj":"T617","obj":"T620"},{"id":"R424","pred":"cc","subj":"T618","obj":"T617"},{"id":"R425","pred":"conj","subj":"T619","obj":"T617"},{"id":"R426","pred":"pobj","subj":"T620","obj":"T616"},{"id":"R427","pred":"punct","subj":"T621","obj":"T609"},{"id":"R428","pred":"ROOT","subj":"T622","obj":"T622"},{"id":"R429","pred":"prep","subj":"T623","obj":"T629"},{"id":"R430","pred":"pobj","subj":"T624","obj":"T623"},{"id":"R431","pred":"punct","subj":"T625","obj":"T629"},{"id":"R432","pred":"advmod","subj":"T626","obj":"T627"},{"id":"R433","pred":"amod","subj":"T627","obj":"T628"},{"id":"R434","pred":"nsubj","subj":"T628","obj":"T629"},{"id":"R435","pred":"ROOT","subj":"T629","obj":"T629"},{"id":"R436","pred":"amod","subj":"T630","obj":"T632"},{"id":"R437","pred":"amod","subj":"T631","obj":"T632"},{"id":"R438","pred":"dobj","subj":"T632","obj":"T629"},{"id":"R439","pred":"punct","subj":"T633","obj":"T629"},{"id":"R440","pred":"prep","subj":"T634","obj":"T642"},{"id":"R441","pred":"pobj","subj":"T635","obj":"T634"},{"id":"R442","pred":"punct","subj":"T636","obj":"T642"},{"id":"R443","pred":"compound","subj":"T637","obj":"T640"},{"id":"R444","pred":"compound","subj":"T638","obj":"T640"},{"id":"R445","pred":"compound","subj":"T639","obj":"T640"},{"id":"R446","pred":"nsubjpass","subj":"T640","obj":"T642"},{"id":"R447","pred":"auxpass","subj":"T641","obj":"T642"},{"id":"R448","pred":"ROOT","subj":"T642","obj":"T642"},{"id":"R449","pred":"prep","subj":"T643","obj":"T642"},{"id":"R450","pred":"amod","subj":"T644","obj":"T645"},{"id":"R451","pred":"pobj","subj":"T645","obj":"T643"},{"id":"R452","pred":"advmod","subj":"T646","obj":"T648"},{"id":"R460","pred":"advmod","subj":"T654","obj":"T656"},{"id":"R461","pred":"amod","subj":"T655","obj":"T656"},{"id":"R462","pred":"nsubjpass","subj":"T656","obj":"T658"},{"id":"R463","pred":"auxpass","subj":"T657","obj":"T658"},{"id":"R464","pred":"ROOT","subj":"T658","obj":"T658"},{"id":"R465","pred":"prep","subj":"T659","obj":"T658"},{"id":"R466","pred":"amod","subj":"T660","obj":"T661"},{"id":"R467","pred":"pobj","subj":"T661","obj":"T659"},{"id":"R468","pred":"acl","subj":"T662","obj":"T661"},{"id":"R469","pred":"agent","subj":"T663","obj":"T662"},{"id":"R470","pred":"compound","subj":"T664","obj":"T665"},{"id":"R471","pred":"pobj","subj":"T665","obj":"T663"},{"id":"R472","pred":"punct","subj":"T666","obj":"T665"},{"id":"R473","pred":"det","subj":"T667","obj":"T670"},{"id":"R474","pred":"amod","subj":"T668","obj":"T670"},{"id":"R475","pred":"compound","subj":"T669","obj":"T670"},{"id":"R476","pred":"appos","subj":"T670","obj":"T665"},{"id":"R477","pred":"punct","subj":"T671","obj":"T665"},{"id":"R478","pred":"punct","subj":"T672","obj":"T658"},{"id":"R479","pred":"nsubj","subj":"T673","obj":"T685"},{"id":"R480","pred":"prep","subj":"T674","obj":"T673"},{"id":"R481","pred":"pobj","subj":"T675","obj":"T674"},{"id":"R482","pred":"prep","subj":"T676","obj":"T673"},{"id":"R483","pred":"amod","subj":"T677","obj":"T680"},{"id":"R484","pred":"nmod","subj":"T678","obj":"T680"},{"id":"R485","pred":"compound","subj":"T679","obj":"T680"},{"id":"R486","pred":"nmod","subj":"T680","obj":"T684"},{"id":"R487","pred":"punct","subj":"T681","obj":"T682"},{"id":"R488","pred":"appos","subj":"T682","obj":"T680"},{"id":"R489","pred":"punct","subj":"T683","obj":"T680"},{"id":"R490","pred":"pobj","subj":"T684","obj":"T676"},{"id":"R491","pred":"ROOT","subj":"T685","obj":"T685"},{"id":"R492","pred":"nmod","subj":"T686","obj":"T688"},{"id":"R493","pred":"amod","subj":"T687","obj":"T688"},{"id":"R494","pred":"dobj","subj":"T688","obj":"T685"},{"id":"R495","pred":"cc","subj":"T689","obj":"T688"},{"id":"R496","pred":"amod","subj":"T690","obj":"T691"},{"id":"R497","pred":"conj","subj":"T691","obj":"T688"},{"id":"R498","pred":"prep","subj":"T692","obj":"T685"},{"id":"R499","pred":"pobj","subj":"T693","obj":"T692"},{"id":"R500","pred":"prep","subj":"T694","obj":"T693"},{"id":"R501","pred":"compound","subj":"T695","obj":"T696"},{"id":"R502","pred":"pobj","subj":"T696","obj":"T694"},{"id":"R503","pred":"prep","subj":"T697","obj":"T696"},{"id":"R504","pred":"amod","subj":"T698","obj":"T699"},{"id":"R505","pred":"pobj","subj":"T699","obj":"T697"},{"id":"R506","pred":"punct","subj":"T700","obj":"T685"},{"id":"R507","pred":"advmod","subj":"T701","obj":"T705"},{"id":"R508","pred":"punct","subj":"T702","obj":"T705"},{"id":"R509","pred":"amod","subj":"T703","obj":"T704"},{"id":"R518","pred":"punct","subj":"T712","obj":"T705"},{"id":"R519","pred":"advmod","subj":"T713","obj":"T716"},{"id":"R520","pred":"det","subj":"T714","obj":"T715"},{"id":"R521","pred":"nsubj","subj":"T715","obj":"T716"},{"id":"R522","pred":"ROOT","subj":"T716","obj":"T716"},{"id":"R523","pred":"mark","subj":"T717","obj":"T731"},{"id":"R524","pred":"nsubjpass","subj":"T718","obj":"T731"},{"id":"R525","pred":"prep","subj":"T719","obj":"T718"},{"id":"R526","pred":"amod","subj":"T720","obj":"T722"},{"id":"R527","pred":"compound","subj":"T721","obj":"T722"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T874","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"},{"id":"T873","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"},{"id":"T872","span":{"begin":1412,"end":1414},"obj":"Protein"},{"id":"T871","span":{"begin":1382,"end":1411},"obj":"Protein"},{"id":"T870","span":{"begin":1224,"end":1232},"obj":"Protein"},{"id":"T869","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T868","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T867","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T866","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T865","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"},{"id":"T864","span":{"begin":1166,"end":1172},"obj":"Protein"},{"id":"T863","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T862","span":{"begin":1066,"end":1074},"obj":"Protein"},{"id":"T861","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T860","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T859","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T858","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T857","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T856","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T855","span":{"begin":888,"end":894},"obj":"Protein"},{"id":"T854","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T853","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T852","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T851","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T850","span":{"begin":666,"end":680},"obj":"Protein"},{"id":"T849","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T846","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T847","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T848","span":{"begin":539,"end":547},"obj":"Protein"}],"relations":[{"id":"R576","pred":"themeOf","subj":"T866","obj":"T868"},{"id":"R577","pred":"themeOf","subj":"T867","obj":"T869"},{"id":"R578","pred":"Cause","subj":"T868","obj":"T865"},{"id":"R579","pred":"Cause","subj":"T869","obj":"T865"},{"id":"R580","pred":"themeOf","subj":"T871","obj":"T873"},{"id":"R581","pred":"themeOf","subj":"T872","obj":"T874"},{"id":"R568","pred":"themeOf","subj":"T852","obj":"T853"},{"id":"R569","pred":"themeOf","subj":"T854","obj":"T856"},{"id":"R570","pred":"themeOf","subj":"T855","obj":"T857"},{"id":"R571","pred":"themeOf","subj":"T856","obj":"T858"},{"id":"R572","pred":"themeOf","subj":"T857","obj":"T859"},{"id":"R573","pred":"themeOf","subj":"T860","obj":"T865"},{"id":"R574","pred":"themeOf","subj":"T863","obj":"T866"},{"id":"R575","pred":"themeOf","subj":"T864","obj":"T867"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"testone","denotations":[{"id":"T19","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T20","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T21","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T22","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T23","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T24","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T25","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T26","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T27","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T28","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T29","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T30","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T31","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T32","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T35","span":{"begin":288,"end":298},"obj":"Negative_regulation"},{"id":"T36","span":{"begin":392,"end":399},"obj":"Positive_regulation"},{"id":"T37","span":{"begin":420,"end":427},"obj":"Positive_regulation"},{"id":"T38","span":{"begin":561,"end":570},"obj":"Positive_regulation"},{"id":"T39","span":{"begin":611,"end":619},"obj":"Gene_expression"},{"id":"T40","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T41","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T42","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T43","span":{"begin":968,"end":978},"obj":"Gene_expression"},{"id":"T44","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"},{"id":"T45","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T46","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T47","span":{"begin":1388,"end":1396},"obj":"Positive_regulation"},{"id":"T48","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"}],"relations":[{"id":"R3","pred":"themeOf","subj":"T20","obj":"T38"},{"id":"R4","pred":"themeOf","subj":"T24","obj":"T40"},{"id":"R5","pred":"themeOf","subj":"T25","obj":"T41"},{"id":"R6","pred":"themeOf","subj":"T26","obj":"T44"},{"id":"R7","pred":"causeOf","subj":"T27","obj":"T44"},{"id":"R8","pred":"themeOf","subj":"T29","obj":"T46"},{"id":"R9","pred":"causeOf","subj":"T31","obj":"T47"},{"id":"R10","pred":"themeOf","subj":"T32","obj":"T48"},{"id":"R12","pred":"themeOf","subj":"T41","obj":"T42"},{"id":"R13","pred":"causeOf","subj":"T43","obj":"T42"},{"id":"R14","pred":"themeOf","subj":"T46","obj":"T45"},{"id":"R15","pred":"themeOf","subj":"T48","obj":"T47"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}

{"project":"test3","denotations":[{"id":"T93","span":{"begin":1415,"end":1430},"obj":"Phosphorylation"},{"id":"T92","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T91","span":{"begin":1388,"end":1396},"obj":"Positive_regulation"},{"id":"T90","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T89","span":{"begin":1224,"end":1232},"obj":"Protein"},{"id":"T88","span":{"begin":1173,"end":1188},"obj":"Phosphorylation"},{"id":"T87","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T86","span":{"begin":1148,"end":1158},"obj":"Negative_regulation"},{"id":"T85","span":{"begin":1066,"end":1074},"obj":"Protein"},{"id":"T84","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T83","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T82","span":{"begin":1013,"end":1027},"obj":"Negative_regulation"},{"id":"T81","span":{"begin":968,"end":978},"obj":"Gene_expression"},{"id":"T80","span":{"begin":915,"end":924},"obj":"Positive_regulation"},{"id":"T79","span":{"begin":895,"end":910},"obj":"Phosphorylation"},{"id":"T78","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T77","span":{"begin":798,"end":808},"obj":"Gene_expression"},{"id":"T76","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T75","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T74","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T73","span":{"begin":561,"end":570},"obj":"Positive_regulation"},{"id":"T72","span":{"begin":539,"end":547},"obj":"Protein"},{"id":"T71","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T51","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T52","span":{"begin":521,"end":537},"obj":"Protein"},{"id":"T53","span":{"begin":540,"end":547},"obj":"Protein"},{"id":"T54","span":{"begin":656,"end":661},"obj":"Protein"},{"id":"T55","span":{"begin":744,"end":749},"obj":"Protein"},{"id":"T56","span":{"begin":781,"end":797},"obj":"Protein"},{"id":"T57","span":{"begin":884,"end":887},"obj":"Protein"},{"id":"T58","span":{"begin":1031,"end":1036},"obj":"Protein"},{"id":"T59","span":{"begin":1048,"end":1064},"obj":"Protein"},{"id":"T60","span":{"begin":1067,"end":1074},"obj":"Protein"},{"id":"T61","span":{"begin":1162,"end":1165},"obj":"Protein"},{"id":"T62","span":{"begin":1225,"end":1232},"obj":"Protein"},{"id":"T63","span":{"begin":1382,"end":1387},"obj":"Protein"},{"id":"T64","span":{"begin":1397,"end":1400},"obj":"Protein"},{"id":"T69","span":{"begin":229,"end":258},"obj":"Protein"},{"id":"T70","span":{"begin":420,"end":427},"obj":"Positive_regulation"}],"relations":[{"id":"R33","pred":"themeOf","subj":"T93","obj":"T91"},{"id":"R27","pred":"causeOf","subj":"T84","obj":"T82"},{"id":"R28","pred":"equivalentTo","subj":"T85","obj":"T84"},{"id":"R29","pred":"themeOf","subj":"T87","obj":"T88"},{"id":"R30","pred":"themeOf","subj":"T88","obj":"T86"},{"id":"R31","pred":"causeOf","subj":"T90","obj":"T91"},{"id":"R32","pred":"themeOf","subj":"T92","obj":"T93"},{"id":"R19","pred":"causeOf","subj":"T71","obj":"T70"},{"id":"R20","pred":"themeOf","subj":"T71","obj":"T73"},{"id":"R21","pred":"equivalentTo","subj":"T72","obj":"T71"},{"id":"R22","pred":"themeOf","subj":"T76","obj":"T77"},{"id":"R23","pred":"themeOf","subj":"T78","obj":"T79"},{"id":"R24","pred":"themeOf","subj":"T79","obj":"T80"},{"id":"R25","pred":"causeOf","subj":"T81","obj":"T80"},{"id":"R26","pred":"themeOf","subj":"T83","obj":"T82"}],"text":"Background\nStatus epilepticus (SE) induces severe vasogenic edema in the piriform cortex (PC) accompanied by neuronal and astroglial damages. To elucidate the mechanism of SE-induced vasogenic edema, we investigated the roles of tumor necrosis factor (TNF)-α in blood-brain barrier (BBB) disruption during vasogenic edema and its related events in rat epilepsy models provoked by pilocarpine-induced SE.\n\nMethods\nSE was induced by pilocarpine in rats that were intracerebroventricularly infused with saline-, and soluble TNF p55 receptor (sTNFp55R) prior to SE induction. Thereafter, we performed Fluoro-Jade B staining and immunohistochemical studies for TNF-α and NF-κB subunits.\n\nResults\nFollowing SE, most activated microglia showed strong TNF-α immunoreactivity. In addition, TNF p75 receptor expression was detected in endothelial cells as well as astrocytes. In addition, only p65-Thr435 phosphorylation was increased in endothelial cells accompanied by SMI-71 expression (an endothelial barrier antigen). Neutralization of TNF-α by soluble TNF p55 receptor (sTNFp55R) infusion attenuated SE-induced vasogenic edema and neuronal damages via inhibition of p65-Thr435 phosphorylation in endothelial cells. Furthermore, sTNFp55R infusion reduced SE-induced neutrophil infiltration in the PC.\n\nConclusion\nThese findings suggest that impairments of endothelial cell functions via TNF-α-mediated p65-Thr 485 NF-κB phosphorylation may be involved in SE-induced vasogenic edema. Subsequently, vasogenic edema results in extensive neutrophil infiltration and neuronal-astroglial loss."}