PMC:3291650 / 36929-37501
Annnotations
pmc-enju-pas
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the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T17081","span":{"begin":196,"end":199},"obj":"http://www.uniprot.org/uniprot/P04141"},{"id":"T17080","span":{"begin":194,"end":199},"obj":"http://www.uniprot.org/uniprot/P09603"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"For the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
UBERON-AE
{"project":"UBERON-AE","denotations":[{"id":"T16542","span":{"begin":376,"end":380},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T16541","span":{"begin":346,"end":353},"obj":"http://purl.obolibrary.org/obo/UBERON_0003126"},{"id":"T16540","span":{"begin":28,"end":39},"obj":"http://purl.obolibrary.org/obo/UBERON_0002371"}],"text":"For the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
GO-BP
{"project":"GO-BP","denotations":[{"id":"T16558","span":{"begin":433,"end":441},"obj":"http://purl.obolibrary.org/obo/GO_0048286"},{"id":"T16557","span":{"begin":320,"end":328},"obj":"http://purl.obolibrary.org/obo/GO_0048286"},{"id":"T16556","span":{"begin":28,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0071838"},{"id":"T16555","span":{"begin":28,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0071839"}],"text":"For the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
GO-MF
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GO-CC
{"project":"GO-CC","denotations":[{"id":"T16564","span":{"begin":287,"end":297},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T16563","span":{"begin":287,"end":297},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T16562","span":{"begin":40,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"For the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
sentences
{"project":"sentences","denotations":[{"id":"T16550","span":{"begin":433,"end":572},"obj":"Sentence"},{"id":"T16549","span":{"begin":299,"end":432},"obj":"Sentence"},{"id":"T16548","span":{"begin":201,"end":298},"obj":"Sentence"},{"id":"T16547","span":{"begin":0,"end":200},"obj":"Sentence"},{"id":"T236","span":{"begin":0,"end":200},"obj":"Sentence"},{"id":"T237","span":{"begin":201,"end":298},"obj":"Sentence"},{"id":"T238","span":{"begin":299,"end":432},"obj":"Sentence"},{"id":"T239","span":{"begin":433,"end":572},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For the generation of BMDM, bone marrow cells were cultured 7 days in RPMI 1640 (Gibco) supplemented with 10% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate, antibiotics and 40 ng/ml recombinant M-CSF. BMDM were of ≥95% purity as measured by flow cytometry using F4/80 and CD11b specific antibodies. For the isolation of alveolar macrophages, the trachea was canulated and the lung was flushed 4 times with HBSS containing 1 mM EDTA. Alveolar macrophages were cultured in RPMI 1640 (Gibco) supplemented with 1% FCS, 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics."}
bionlp-st-ge-2016-test-ihmc
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bionlp-st-ge-2016-spacy-parsed
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bionlp-st-ge-2016-test-tees
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