PMC:3291650 / 35445-36071 JSONTXT

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    test3

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    2_test

    {"project":"2_test","denotations":[{"id":"22396652-21187318-98461893","span":{"begin":156,"end":158},"obj":"21187318"}],"text":"Granzyme B (GrB) and IFNγ expressing CD8+ T cells were determined by treating the mice intranasally with 50 µg Brefeldin A (Sigma) as previously described [86]. 6 h later, BAL and lungs were isolated and single cell suspensions were prepared from the lung in the presence of 3 µg/ml Brefeldin A. Cells were stained, fixed and permeabilized (Cytofix/Cytoperm, BD Biosciences) according to the manufacturer's instructions. Activated CD8+ T cells were analyzed by flow cytometry based on CD62lo CD3+ and CD8+ expression. Live/Dead fixable aqua dead cell stain kit (Molecular Probes) was used to discriminate live from dead cells."}

    pmc-enju-pas

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B (GrB) and IFNγ expressing CD8+ T cells were determined by treating the mice intranasally with 50 µg Brefeldin A (Sigma) as previously described [86]. 6 h later, BAL and lungs were isolated and single cell suspensions were prepared from the lung in the presence of 3 µg/ml Brefeldin A. Cells were stained, fixed and permeabilized (Cytofix/Cytoperm, BD Biosciences) according to the manufacturer's instructions. Activated CD8+ T cells were analyzed by flow cytometry based on CD62lo CD3+ and CD8+ expression. Live/Dead fixable aqua dead cell stain kit (Molecular Probes) was used to discriminate live from dead cells."}

    bionlp-st-ge-2016-test-proteins

    {"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T16132","span":{"begin":501,"end":504},"obj":"Protein"},{"id":"T16131","span":{"begin":492,"end":495},"obj":"Protein"},{"id":"T16130","span":{"begin":431,"end":434},"obj":"Protein"},{"id":"T16129","span":{"begin":37,"end":40},"obj":"Protein"},{"id":"T16128","span":{"begin":12,"end":15},"obj":"Protein"},{"id":"T16127","span":{"begin":0,"end":10},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Granzyme B (GrB) and IFNγ expressing CD8+ T cells were determined by treating the mice intranasally with 50 µg Brefeldin A (Sigma) as previously described [86]. 6 h later, BAL and lungs were isolated and single cell suspensions were prepared from the lung in the presence of 3 µg/ml Brefeldin A. Cells were stained, fixed and permeabilized (Cytofix/Cytoperm, BD Biosciences) according to the manufacturer's instructions. Activated CD8+ T cells were analyzed by flow cytometry based on CD62lo CD3+ and CD8+ expression. Live/Dead fixable aqua dead cell stain kit (Molecular Probes) was used to discriminate live from dead cells."}

    bionlp-st-ge-2016-uniprot

    {"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T16416","span":{"begin":492,"end":495},"obj":"http://www.uniprot.org/uniprot/P09693"},{"id":"T16415","span":{"begin":492,"end":495},"obj":"http://www.uniprot.org/uniprot/P07766"},{"id":"T16414","span":{"begin":492,"end":495},"obj":"http://www.uniprot.org/uniprot/P04234"},{"id":"T16413","span":{"begin":492,"end":495},"obj":"http://www.uniprot.org/uniprot/P20963"},{"id":"T16412","span":{"begin":501,"end":504},"obj":"http://www.uniprot.org/uniprot/P10966"},{"id":"T16411","span":{"begin":431,"end":434},"obj":"http://www.uniprot.org/uniprot/P10966"},{"id":"T16410","span":{"begin":37,"end":40},"obj":"http://www.uniprot.org/uniprot/P10966"},{"id":"T16408","span":{"begin":501,"end":504},"obj":"http://www.uniprot.org/uniprot/P01732"},{"id":"T16407","span":{"begin":431,"end":434},"obj":"http://www.uniprot.org/uniprot/P01732"},{"id":"T16406","span":{"begin":37,"end":40},"obj":"http://www.uniprot.org/uniprot/P01732"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Granzyme B (GrB) and IFNγ expressing CD8+ T cells were determined by treating the mice intranasally with 50 µg Brefeldin A (Sigma) as previously described [86]. 6 h later, BAL and lungs were isolated and single cell suspensions were prepared from the lung in the presence of 3 µg/ml Brefeldin A. Cells were stained, fixed and permeabilized (Cytofix/Cytoperm, BD Biosciences) according to the manufacturer's instructions. Activated CD8+ T cells were analyzed by flow cytometry based on CD62lo CD3+ and CD8+ expression. Live/Dead fixable aqua dead cell stain kit (Molecular Probes) was used to discriminate live from dead cells."}

    UBERON-AE

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    GO-CC

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    sentences

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    simple1

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    BioNLP16_DUT

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    BioNLP16_Messiy

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    DLUT931

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    bionlp-st-ge-2016-test-ihmc

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    bionlp-st-ge-2016-spacy-parsed

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