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pmc-enju-pas

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T15281","span":{"begin":797,"end":802},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T15280","span":{"begin":606,"end":610},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T15279","span":{"begin":503,"end":508},"obj":"http://purl.obolibrary.org/obo/UBERON_0002048"},{"id":"T15278","span":{"begin":438,"end":444},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T15290","span":{"begin":0,"end":15},"obj":"http://purl.obolibrary.org/obo/GO_0016032"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T15294","span":{"begin":803,"end":808},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T15293","span":{"begin":646,"end":651},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T15292","span":{"begin":124,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

sentences

{"project":"sentences","denotations":[{"id":"T15289","span":{"begin":884,"end":967},"obj":"Sentence"},{"id":"T15288","span":{"begin":766,"end":883},"obj":"Sentence"},{"id":"T15287","span":{"begin":575,"end":765},"obj":"Sentence"},{"id":"T15286","span":{"begin":394,"end":574},"obj":"Sentence"},{"id":"T15285","span":{"begin":305,"end":393},"obj":"Sentence"},{"id":"T15284","span":{"begin":131,"end":304},"obj":"Sentence"},{"id":"T15283","span":{"begin":50,"end":130},"obj":"Sentence"},{"id":"T15282","span":{"begin":0,"end":49},"obj":"Sentence"},{"id":"T209","span":{"begin":0,"end":49},"obj":"Sentence"},{"id":"T210","span":{"begin":50,"end":130},"obj":"Sentence"},{"id":"T211","span":{"begin":131,"end":304},"obj":"Sentence"},{"id":"T212","span":{"begin":305,"end":393},"obj":"Sentence"},{"id":"T213","span":{"begin":394,"end":574},"obj":"Sentence"},{"id":"T214","span":{"begin":575,"end":765},"obj":"Sentence"},{"id":"T215","span":{"begin":766,"end":883},"obj":"Sentence"},{"id":"T216","span":{"begin":884,"end":967},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

ICD10

{"project":"ICD10","denotations":[{"id":"T15291","span":{"begin":0,"end":15},"obj":"http://purl.bioontology.org/ontology/ICD10/B34.9"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T15656","span":{"begin":641,"end":696},"obj":"Entity"},{"id":"T15655","span":{"begin":708,"end":724},"obj":"Entity"},{"id":"T15654","span":{"begin":726,"end":748},"obj":"Entity"},{"id":"T15653","span":{"begin":780,"end":814},"obj":"Entity"},{"id":"T15652","span":{"begin":810,"end":813},"obj":"Entity"},{"id":"T15651","span":{"begin":154,"end":158},"obj":"Protein"},{"id":"T15650","span":{"begin":689,"end":696},"obj":"Entity"},{"id":"T15649","span":{"begin":341,"end":345},"obj":"Protein"},{"id":"T15648","span":{"begin":200,"end":219},"obj":"Entity"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T15645","span":{"begin":689,"end":696},"obj":"Protein"},{"id":"T15644","span":{"begin":119,"end":123},"obj":"Protein"}],"text":"Viral infection and determination of viral titers\nMouse adapted IAV X-47 (H3N2; PR8×A/Victoria/3/75) was propagated in MDCK cells. For viral inoculation, mice were anesthetized by i.p. injection with ketamine (12 mg/kg) and xylazine (60 mg/kg) and 50 µl X-47 diluted in PBS was administered intranasally. For lethal and sublethal infection, mice received respectively 2-LD50 or 0.05-LD50 X-47. To determine pulmonary viral titers, median tissue culture infectious dose (TCID50) was measured as follows: lungs were homogenized with a Polytron homogenizer (Kinematica) in PBS. Eight-fold serial dilutions of lung homogenates were incubated on MDCK cells for 5 days in DMEM supplemented with trypsin (1 µg/ml), 2 mM L-glutamine, 0.4 mM sodium pyruvate and antibiotics. For read-out, 0.5% chicken red blood cells (RBC) were added and end-point dilution of hemagglutination was monitored. TCID50 titers were then calculated according to the method of Reed and Muench [85]."}

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PMC:3291650 / 33398-34365 JSON TXT