PMC:329117 / 32660-34319 JSONTXT

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Often, the coding region of olfactory receptors is highly similar between recently duplicated genes. Many pairs of similar olfactory receptors show more sequence divergence in the UTRs than the protein-coding region (J.Y., unpublished observations). The UTRs would therefore make a better choice of sequence from which to design unique oligonucleotides to distinguish closely related olfactory receptor genes. Locations of these regions in genomic sequence are difficult to predict - our study provides 5' UTR sequences of 343 genes and the approximate 3' UTR length for 399 olfactory receptor genes. Probe design must also account for the multiple transcriptional isoforms observed for many olfactory receptors - depending on the question being asked, probes could be designed in shared sequence to determine the total level of all isoforms, or in unique exons to measure the level of each isoform 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