PMC:3279418 / 30627-30970
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11679 | 0-7 | NNP | denotes | Reverse |
T11680 | 8-21 | NN | denotes | transcription |
T11681 | 22-25 | VB | denotes | was |
T11682 | 26-35 | VB | denotes | performed |
T11683 | 36-38 | IN | denotes | at |
T11684 | 39-43 | NN | denotes | 42°C |
T11685 | 44-47 | IN | denotes | for |
T11686 | 48-50 | CD | denotes | 60 |
T11687 | 51-58 | NN | denotes | minutes |
T11688 | 59-63 | IN | denotes | with |
T11689 | 64-67 | DT | denotes | the |
T11690 | 68-73 | JJ | denotes | final |
T11691 | 74-86 | NN | denotes | denaturation |
T11692 | 87-91 | NN | denotes | step |
T11693 | 92-94 | IN | denotes | at |
T11694 | 95-99 | NN | denotes | 90°C |
T11695 | 100-103 | IN | denotes | for |
T11696 | 104-105 | CD | denotes | 5 |
T11697 | 106-113 | NN | denotes | minutes |
T11698 | 114-116 | IN | denotes | in |
T11699 | 117-119 | CD | denotes | 30 |
T11700 | 120-122 | NN | denotes | µl |
T11701 | 123-133 | VB | denotes | containing |
T11702 | 134-137 | CD | denotes | 0.5 |
T11703 | 138-140 | NN | denotes | µg |
T11704 | 141-143 | IN | denotes | of |
T11705 | 144-149 | JJ | denotes | total |
T11706 | 150-154 | NN | denotes | RNA. |
T11707 | 155-160 | NN | denotes | dNTPs |
T11708 | 160-161 | -COMMA- | denotes | , |
T11709 | 162-167 | NN | denotes | oligo |
T11710 | 167-168 | -LRB- | denotes | ( |
T11711 | 168-170 | NN | denotes | dT |
T11712 | 170-171 | -RRB- | denotes | ) |
T11713 | 171-173 | CD | denotes | 15 |
T11714 | 174-180 | NN | denotes | primer |
T11715 | 180-181 | -COMMA- | denotes | , |
T11716 | 182-193 | JJ | denotes | recombinant |
T11717 | 194-200 | NNP | denotes | RNasin |
T11718 | 201-213 | NNP | denotes | Ribonuclease |
T11719 | 214-223 | NNP | denotes | Inhibitor |
T11720 | 223-224 | -COMMA- | denotes | , |
T11721 | 225-228 | CC | denotes | and |
T11722 | 229-234 | NN | denotes | M-MLV |
T11723 | 235-242 | JJ | denotes | Reverse |
T11724 | 243-256 | NN | denotes | Transcriptase |
T11725 | 257-258 | -LRB- | denotes | ( |
T11726 | 258-261 | DT | denotes | all |
T11727 | 262-266 | IN | denotes | from |
T11728 | 267-274 | NNP | denotes | Promega |
T11729 | 274-275 | -COMMA- | denotes | , |
T11730 | 276-283 | NNP | denotes | Madison |
T11731 | 283-284 | -COMMA- | denotes | , |
T11732 | 285-287 | NNP | denotes | WI |
T11733 | 287-288 | -RRB- | denotes | ) |
T11734 | 289-293 | VB | denotes | were |
T11735 | 294-298 | VB | denotes | used |
T11736 | 299-308 | VB | denotes | according |
T11737 | 309-311 | TO | denotes | to |
T11738 | 312-315 | DT | denotes | the |
T11739 | 316-328 | NN | denotes | manufacturer |
T11740 | 328-330 | POS | denotes | 's |
T11741 | 331-342 | NN | denotes | instruction |
R9719 | T11679 | T11701 | arg1Of | Reverse,containing |
R9720 | T11680 | T11681 | arg1Of | transcription,was |
R9721 | T11680 | T11682 | arg2Of | transcription,performed |
R9722 | T11682 | T11681 | arg2Of | performed,was |
R9723 | T11682 | T11683 | arg1Of | performed,at |
R9724 | T11682 | T11685 | arg1Of | performed,for |
R9725 | T11682 | T11688 | arg1Of | performed,with |
R9726 | T11684 | T11683 | arg2Of | 42°C,at |
R9727 | T11687 | T11685 | arg2Of | minutes,for |
R9728 | T11687 | T11686 | arg1Of | minutes,60 |
R9729 | T11692 | T11688 | arg2Of | step,with |
R9730 | T11692 | T11689 | arg1Of | step,the |
R9731 | T11692 | T11690 | arg1Of | step,final |
R9732 | T11692 | T11691 | arg1Of | step,denaturation |
R9733 | T11692 | T11693 | arg1Of | step,at |
R9734 | T11692 | T11695 | arg1Of | step,for |
R9735 | T11692 | T11698 | arg1Of | step,in |
R9736 | T11694 | T11693 | arg2Of | 90°C,at |
R9737 | T11697 | T11695 | arg2Of | minutes,for |
R9738 | T11697 | T11696 | arg1Of | minutes,5 |
R9739 | T11700 | T11698 | arg2Of | µl,in |
R9740 | T11700 | T11699 | arg1Of | µl,30 |
R9741 | T11701 | T11681 | modOf | containing,was |
R9742 | T11703 | T11701 | arg2Of | µg,containing |
R9743 | T11703 | T11702 | arg1Of | µg,0.5 |
R9744 | T11703 | T11704 | arg1Of | µg,of |
R9745 | T11707 | T11704 | arg2Of | dNTPs,of |
R9746 | T11707 | T11705 | arg1Of | dNTPs,total |
R9747 | T11707 | T11706 | arg1Of | dNTPs,RNA. |
R9748 | T11707 | T11708 | arg1Of | dNTPs,"," |
R9749 | T11709 | T11708 | arg2Of | oligo,"," |
R9750 | T11709 | T11710 | arg1Of | oligo,( |
R9751 | T11711 | T11710 | arg2Of | dT,( |
R9752 | T11712 | T11710 | arg3Of | ),( |
R9754 | T11714 | T11679 | arg1Of | primer,Reverse |
R9755 | T11714 | T11713 | arg1Of | primer,15 |
R9756 | T11714 | T11715 | arg1Of | primer,"," |
R9757 | T11719 | T11715 | arg2Of | Inhibitor,"," |
R9758 | T11719 | T11716 | arg1Of | Inhibitor,recombinant |
R9759 | T11719 | T11717 | arg1Of | Inhibitor,RNasin |
R9760 | T11719 | T11718 | arg1Of | Inhibitor,Ribonuclease |
R9761 | T11721 | T11720 | arg1Of | and,"," |
R9762 | T11724 | T11722 | arg1Of | Transcriptase,M-MLV |
R9763 | T11724 | T11723 | arg1Of | Transcriptase,Reverse |
R9764 | T11724 | T11725 | arg1Of | Transcriptase,( |
R9765 | T11724 | T11734 | arg1Of | Transcriptase,were |
R9766 | T11724 | T11735 | arg2Of | Transcriptase,used |
R9767 | T11726 | T11725 | arg2Of | all,( |
R9768 | T11726 | T11727 | arg1Of | all,from |
R9769 | T11726 | T11729 | arg1Of | all,"," |
R9770 | T11728 | T11727 | arg2Of | Promega,from |
R9771 | T11730 | T11729 | arg2Of | Madison,"," |
R9772 | T11730 | T11731 | arg1Of | Madison,"," |
R9773 | T11732 | T11731 | arg2Of | WI,"," |
R9774 | T11733 | T11725 | arg3Of | ),( |
R9775 | T11735 | T11721 | arg2Of | used,and |
R9776 | T11735 | T11734 | arg2Of | used,were |
R9777 | T11735 | T11736 | arg1Of | used,according |
R9778 | T11737 | T11736 | arg2Of | to,according |
R9779 | T11739 | T11738 | arg1Of | manufacturer,the |
R9780 | T11739 | T11740 | arg2Of | manufacturer,'s |
R9781 | T11741 | T11737 | arg2Of | instruction,to |
R9782 | T11741 | T11740 | arg1Of | instruction,'s |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11441 | 0-7 | VB | denotes | Reverse |
T11442 | 8-21 | NN | denotes | transcription |
T11443 | 22-25 | VBD | denotes | was |
T11444 | 26-35 | VBN | denotes | performed |
T11445 | 36-38 | IN | denotes | at |
T11446 | 39-41 | CD | denotes | 42 |
T11447 | 41-42 | CD | denotes | ° |
T11448 | 42-43 | NNP | denotes | C |
T11449 | 44-47 | IN | denotes | for |
T11450 | 48-50 | CD | denotes | 60 |
T11451 | 51-58 | NNS | denotes | minutes |
T11452 | 59-63 | IN | denotes | with |
T11453 | 64-67 | DT | denotes | the |
T11454 | 68-73 | JJ | denotes | final |
T11455 | 74-86 | NN | denotes | denaturation |
T11456 | 87-91 | NN | denotes | step |
T11457 | 92-94 | IN | denotes | at |
T11458 | 95-97 | CD | denotes | 90 |
T11459 | 97-98 | CD | denotes | ° |
T11460 | 98-99 | NNP | denotes | C |
T11461 | 100-103 | IN | denotes | for |
T11462 | 104-105 | CD | denotes | 5 |
T11463 | 106-113 | NNS | denotes | minutes |
T11464 | 114-116 | IN | denotes | in |
T11465 | 117-119 | CD | denotes | 30 |
T11466 | 120-122 | NN | denotes | µl |
T11467 | 123-133 | VBG | denotes | containing |
T11468 | 134-137 | CD | denotes | 0.5 |
T11469 | 138-140 | NN | denotes | µg |
T11470 | 141-143 | IN | denotes | of |
T11471 | 144-149 | JJ | denotes | total |
T11472 | 150-153 | NNP | denotes | RNA |
T11473 | 153-154 | . | denotes | . |
T11474 | 155-160 | NNS | denotes | dNTPs |
T11475 | 160-161 | , | denotes | , |
T11476 | 162-167 | NN | denotes | oligo |
T11477 | 167-168 | -LRB- | denotes | ( |
T11478 | 168-170 | NN | denotes | dT |
T11479 | 170-171 | -RRB- | denotes | ) |
T11480 | 171-173 | CD | denotes | 15 |
T11481 | 174-180 | NN | denotes | primer |
T11482 | 180-181 | , | denotes | , |
T11483 | 182-193 | JJ | denotes | recombinant |
T11484 | 194-200 | NNP | denotes | RNasin |
T11485 | 201-213 | NNP | denotes | Ribonuclease |
T11486 | 214-223 | NNP | denotes | Inhibitor |
T11487 | 223-224 | , | denotes | , |
T11488 | 225-228 | CC | denotes | and |
T11489 | 229-234 | NNP | denotes | M-MLV |
T11490 | 235-242 | VB | denotes | Reverse |
T11491 | 243-256 | NNP | denotes | Transcriptase |
T11492 | 257-258 | -LRB- | denotes | ( |
T11493 | 258-261 | DT | denotes | all |
T11494 | 262-266 | IN | denotes | from |
T11495 | 267-274 | NNP | denotes | Promega |
T11496 | 274-275 | , | denotes | , |
T11497 | 276-283 | NNP | denotes | Madison |
T11498 | 283-284 | , | denotes | , |
T11499 | 285-287 | NNP | denotes | WI |
T11500 | 287-288 | -RRB- | denotes | ) |
T11501 | 289-293 | VBD | denotes | were |
T11502 | 294-298 | VBN | denotes | used |
T11503 | 299-308 | VBG | denotes | according |
T11504 | 309-311 | TO | denotes | to |
T11505 | 312-315 | DT | denotes | the |
T11506 | 316-328 | NN | denotes | manufacturer |
T11507 | 328-330 | POS | denotes | 's |
T11508 | 331-342 | NN | denotes | instruction |
T11509 | 342-343 | . | denotes | . |
R9479 | T11441 | T11442 | amod | Reverse,transcription |
R9480 | T11442 | T11444 | nsubjpass | transcription,performed |
R9481 | T11443 | T11444 | auxpass | was,performed |
R9482 | T11444 | T11444 | ROOT | performed,performed |
R9483 | T11445 | T11444 | prep | at,performed |
R9484 | T11446 | T11447 | nummod | 42,° |
R9485 | T11447 | T11445 | pobj | °,at |
R9486 | T11448 | T11444 | npadvmod | C,performed |
R9487 | T11449 | T11444 | prep | for,performed |
R9488 | T11450 | T11451 | nummod | 60,minutes |
R9489 | T11451 | T11449 | pobj | minutes,for |
R9490 | T11452 | T11451 | prep | with,minutes |
R9491 | T11453 | T11456 | det | the,step |
R9492 | T11454 | T11456 | amod | final,step |
R9493 | T11455 | T11456 | compound | denaturation,step |
R9494 | T11456 | T11452 | pobj | step,with |
R9495 | T11457 | T11456 | prep | at,step |
R9496 | T11458 | T11459 | nummod | 90,° |
R9497 | T11459 | T11457 | pobj | °,at |
R9498 | T11460 | T11461 | compound | C,for |
R9499 | T11461 | T11444 | prep | for,performed |
R9500 | T11462 | T11463 | nummod | 5,minutes |
R9501 | T11463 | T11461 | pobj | minutes,for |
R9502 | T11464 | T11463 | prep | in,minutes |
R9503 | T11465 | T11466 | nummod | 30,µl |
R9504 | T11466 | T11464 | pobj | µl,in |
R9505 | T11467 | T11466 | acl | containing,µl |
R9506 | T11468 | T11469 | nummod | 0.5,µg |
R9507 | T11469 | T11467 | dobj | µg,containing |
R9508 | T11470 | T11469 | prep | of,µg |
R9509 | T11471 | T11472 | amod | total,RNA |
R9510 | T11472 | T11470 | pobj | RNA,of |
R9511 | T11473 | T11444 | punct | .,performed |
R9512 | T11474 | T11502 | nsubjpass | dNTPs,used |
R9513 | T11475 | T11474 | punct | ",",dNTPs |
R9514 | T11476 | T11474 | conj | oligo,dNTPs |
R9515 | T11477 | T11478 | punct | (,dT |
R9516 | T11478 | T11481 | nmod | dT,primer |
R9517 | T11479 | T11478 | punct | ),dT |
R9518 | T11480 | T11481 | nummod | 15,primer |
R9519 | T11481 | T11474 | appos | primer,dNTPs |
R9520 | T11482 | T11481 | punct | ",",primer |
R9521 | T11483 | T11486 | amod | recombinant,Inhibitor |
R9522 | T11484 | T11486 | compound | RNasin,Inhibitor |
R9523 | T11485 | T11486 | compound | Ribonuclease,Inhibitor |
R9524 | T11486 | T11481 | appos | Inhibitor,primer |
R9525 | T11487 | T11481 | punct | ",",primer |
R9526 | T11488 | T11481 | cc | and,primer |
R9527 | T11489 | T11490 | nsubj | M-MLV,Reverse |
R9528 | T11490 | T11502 | advmod | Reverse,used |
R9529 | T11491 | T11490 | npadvmod | Transcriptase,Reverse |
R9530 | T11492 | T11493 | punct | (,all |
R9531 | T11493 | T11494 | advmod | all,from |
R9532 | T11494 | T11490 | prep | from,Reverse |
R9533 | T11495 | T11494 | pobj | Promega,from |
R9534 | T11496 | T11495 | punct | ",",Promega |
R9535 | T11497 | T11495 | conj | Madison,Promega |
R9536 | T11498 | T11497 | punct | ",",Madison |
R9537 | T11499 | T11497 | conj | WI,Madison |
R9538 | T11500 | T11490 | punct | ),Reverse |
R9539 | T11501 | T11502 | auxpass | were,used |
R9540 | T11502 | T11502 | ROOT | used,used |
R9541 | T11503 | T11502 | prep | according,used |
R9542 | T11504 | T11503 | prep | to,according |
R9543 | T11505 | T11506 | det | the,manufacturer |
R9544 | T11506 | T11508 | poss | manufacturer,instruction |
R9545 | T11507 | T11506 | case | 's,manufacturer |
R9546 | T11508 | T11504 | pobj | instruction,to |
R9547 | T11509 | T11502 | punct | .,used |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11843 | 0-21 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | Reverse transcription |
T11845 | 8-21 | http://purl.obolibrary.org/obo/GO_0006351 | denotes | transcription |
T11847 | 243-256 | http://purl.obolibrary.org/obo/GO_0003899 | denotes | Transcriptase |
T11848 | 243-256 | http://purl.obolibrary.org/obo/GO_0003968 | denotes | Transcriptase |
T11849 | 243-256 | http://purl.obolibrary.org/obo/GO_0034062 | denotes | Transcriptase |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11853 | 243-256 | http://purl.obolibrary.org/obo/GO_0003899 | denotes | Transcriptase |
T11854 | 243-256 | http://purl.obolibrary.org/obo/GO_0003968 | denotes | Transcriptase |
T11855 | 243-256 | http://purl.obolibrary.org/obo/GO_0034062 | denotes | Transcriptase |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T216 | 0-343 | Sentence | denotes | Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11898 | 229-256 | Protein | denotes | M-MLV Reverse Transcriptase |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11913 | 194-213 | Protein | denotes | RNasin Ribonuclease |
T11914 | 229-256 | Protein | denotes | M-MLV Reverse Transcriptase |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11343 | 229-256 | Protein | denotes | M-MLV Reverse Transcriptase |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T11327 | 229-256 | Protein | denotes | M-MLV Reverse Transcriptase |