PMC:3279418 / 30568-30970
Annnotations
pmc-enju-pas
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GO-BP
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GO-MF
{"project":"GO-MF","denotations":[{"id":"T11853","span":{"begin":302,"end":315},"obj":"http://purl.obolibrary.org/obo/GO_0003899"},{"id":"T11854","span":{"begin":302,"end":315},"obj":"http://purl.obolibrary.org/obo/GO_0003968"},{"id":"T11855","span":{"begin":302,"end":315},"obj":"http://purl.obolibrary.org/obo/GO_0034062"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}
sentences
{"project":"sentences","denotations":[{"id":"T11354","span":{"begin":0,"end":402},"obj":"Sentence"},{"id":"T215","span":{"begin":0,"end":58},"obj":"Sentence"},{"id":"T216","span":{"begin":59,"end":402},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T11898","span":{"begin":288,"end":315},"obj":"Protein"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T11913","span":{"begin":253,"end":272},"obj":"Protein"},{"id":"T11914","span":{"begin":288,"end":315},"obj":"Protein"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T11343","span":{"begin":288,"end":315},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}
test2
{"project":"test2","denotations":[{"id":"T11327","span":{"begin":288,"end":315},"obj":"Protein"}],"text":"Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction."}