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PMC:3279418 / 30568-30970 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T11671 0-7 NN denotes Primers
T11672 8-11 CC denotes and
T11673 12-18 NN denotes probes
T11674 19-23 VB denotes were
T11675 24-33 VB denotes purchased
T11676 34-38 IN denotes from
T11677 39-46 NNP denotes Applied
T11678 47-58 NNP denotes Biosystems.
T11679 59-66 NNP denotes Reverse
T11680 67-80 NN denotes transcription
T11681 81-84 VB denotes was
T11682 85-94 VB denotes performed
T11683 95-97 IN denotes at
T11684 98-102 NN denotes 42°C
T11685 103-106 IN denotes for
T11686 107-109 CD denotes 60
T11687 110-117 NN denotes minutes
T11688 118-122 IN denotes with
T11689 123-126 DT denotes the
T11690 127-132 JJ denotes final
T11691 133-145 NN denotes denaturation
T11692 146-150 NN denotes step
T11693 151-153 IN denotes at
T11694 154-158 NN denotes 90°C
T11695 159-162 IN denotes for
T11696 163-164 CD denotes 5
T11697 165-172 NN denotes minutes
T11698 173-175 IN denotes in
T11699 176-178 CD denotes 30
T11700 179-181 NN denotes µl
T11701 182-192 VB denotes containing
T11702 193-196 CD denotes 0.5
T11703 197-199 NN denotes µg
T11704 200-202 IN denotes of
T11705 203-208 JJ denotes total
T11706 209-213 NN denotes RNA.
T11707 214-219 NN denotes dNTPs
T11708 219-220 -COMMA- denotes ,
T11709 221-226 NN denotes oligo
T11710 226-227 -LRB- denotes (
T11711 227-229 NN denotes dT
T11712 229-230 -RRB- denotes )
T11713 230-232 CD denotes 15
T11714 233-239 NN denotes primer
T11715 239-240 -COMMA- denotes ,
T11716 241-252 JJ denotes recombinant
T11717 253-259 NNP denotes RNasin
T11718 260-272 NNP denotes Ribonuclease
T11719 273-282 NNP denotes Inhibitor
T11720 282-283 -COMMA- denotes ,
T11721 284-287 CC denotes and
T11722 288-293 NN denotes M-MLV
T11723 294-301 JJ denotes Reverse
T11724 302-315 NN denotes Transcriptase
T11725 316-317 -LRB- denotes (
T11726 317-320 DT denotes all
T11727 321-325 IN denotes from
T11728 326-333 NNP denotes Promega
T11729 333-334 -COMMA- denotes ,
T11730 335-342 NNP denotes Madison
T11731 342-343 -COMMA- denotes ,
T11732 344-346 NNP denotes WI
T11733 346-347 -RRB- denotes )
T11734 348-352 VB denotes were
T11735 353-357 VB denotes used
T11736 358-367 VB denotes according
T11737 368-370 TO denotes to
T11738 371-374 DT denotes the
T11739 375-387 NN denotes manufacturer
T11740 387-389 POS denotes 's
T11741 390-401 NN denotes instruction
R9710 T11671 T11672 arg1Of Primers,and
R9711 T11672 T11674 arg1Of and,were
R9712 T11672 T11675 arg2Of and,purchased
R9713 T11673 T11672 arg2Of probes,and
R9714 T11675 T11674 arg2Of purchased,were
R9715 T11675 T11676 arg1Of purchased,from
R9716 T11675 T11721 arg1Of purchased,and
R9717 T11679 T11677 arg1Of Reverse,Applied
R9718 T11679 T11678 arg1Of Reverse,Biosystems.
R9719 T11679 T11701 arg1Of Reverse,containing
R9720 T11680 T11681 arg1Of transcription,was
R9721 T11680 T11682 arg2Of transcription,performed
R9722 T11682 T11681 arg2Of performed,was
R9723 T11682 T11683 arg1Of performed,at
R9724 T11682 T11685 arg1Of performed,for
R9725 T11682 T11688 arg1Of performed,with
R9726 T11684 T11683 arg2Of 42°C,at
R9727 T11687 T11685 arg2Of minutes,for
R9728 T11687 T11686 arg1Of minutes,60
R9729 T11692 T11688 arg2Of step,with
R9730 T11692 T11689 arg1Of step,the
R9731 T11692 T11690 arg1Of step,final
R9732 T11692 T11691 arg1Of step,denaturation
R9733 T11692 T11693 arg1Of step,at
R9734 T11692 T11695 arg1Of step,for
R9735 T11692 T11698 arg1Of step,in
R9736 T11694 T11693 arg2Of 90°C,at
R9737 T11697 T11695 arg2Of minutes,for
R9738 T11697 T11696 arg1Of minutes,5
R9739 T11700 T11698 arg2Of µl,in
R9740 T11700 T11699 arg1Of µl,30
R9741 T11701 T11681 modOf containing,was
R9742 T11703 T11701 arg2Of µg,containing
R9743 T11703 T11702 arg1Of µg,0.5
R9744 T11703 T11704 arg1Of µg,of
R9745 T11707 T11704 arg2Of dNTPs,of
R9746 T11707 T11705 arg1Of dNTPs,total
R9747 T11707 T11706 arg1Of dNTPs,RNA.
R9748 T11707 T11708 arg1Of dNTPs,","
R9749 T11709 T11708 arg2Of oligo,","
R9750 T11709 T11710 arg1Of oligo,(
R9751 T11711 T11710 arg2Of dT,(
R9752 T11712 T11710 arg3Of ),(
R9753 T11714 T11676 arg2Of primer,from
R9754 T11714 T11679 arg1Of primer,Reverse
R9755 T11714 T11713 arg1Of primer,15
R9756 T11714 T11715 arg1Of primer,","
R9757 T11719 T11715 arg2Of Inhibitor,","
R9758 T11719 T11716 arg1Of Inhibitor,recombinant
R9759 T11719 T11717 arg1Of Inhibitor,RNasin
R9760 T11719 T11718 arg1Of Inhibitor,Ribonuclease
R9761 T11721 T11720 arg1Of and,","
R9762 T11724 T11722 arg1Of Transcriptase,M-MLV
R9763 T11724 T11723 arg1Of Transcriptase,Reverse
R9764 T11724 T11725 arg1Of Transcriptase,(
R9765 T11724 T11734 arg1Of Transcriptase,were
R9766 T11724 T11735 arg2Of Transcriptase,used
R9767 T11726 T11725 arg2Of all,(
R9768 T11726 T11727 arg1Of all,from
R9769 T11726 T11729 arg1Of all,","
R9770 T11728 T11727 arg2Of Promega,from
R9771 T11730 T11729 arg2Of Madison,","
R9772 T11730 T11731 arg1Of Madison,","
R9773 T11732 T11731 arg2Of WI,","
R9774 T11733 T11725 arg3Of ),(
R9775 T11735 T11721 arg2Of used,and
R9776 T11735 T11734 arg2Of used,were
R9777 T11735 T11736 arg1Of used,according
R9778 T11737 T11736 arg2Of to,according
R9779 T11739 T11738 arg1Of manufacturer,the
R9780 T11739 T11740 arg2Of manufacturer,'s
R9781 T11741 T11737 arg2Of instruction,to
R9782 T11741 T11740 arg1Of instruction,'s

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T11432 0-7 NNS denotes Primers
T11433 8-11 CC denotes and
T11434 12-18 NNS denotes probes
T11435 19-23 VBD denotes were
T11436 24-33 VBN denotes purchased
T11437 34-38 IN denotes from
T11438 39-46 NNP denotes Applied
T11439 47-57 NNPS denotes Biosystems
T11440 57-58 . denotes .
T11441 59-66 VB denotes Reverse
T11442 67-80 NN denotes transcription
T11443 81-84 VBD denotes was
T11444 85-94 VBN denotes performed
T11445 95-97 IN denotes at
T11446 98-100 CD denotes 42
T11447 100-101 CD denotes °
T11448 101-102 NNP denotes C
T11449 103-106 IN denotes for
T11450 107-109 CD denotes 60
T11451 110-117 NNS denotes minutes
T11452 118-122 IN denotes with
T11453 123-126 DT denotes the
T11454 127-132 JJ denotes final
T11455 133-145 NN denotes denaturation
T11456 146-150 NN denotes step
T11457 151-153 IN denotes at
T11458 154-156 CD denotes 90
T11459 156-157 CD denotes °
T11460 157-158 NNP denotes C
T11461 159-162 IN denotes for
T11462 163-164 CD denotes 5
T11463 165-172 NNS denotes minutes
T11464 173-175 IN denotes in
T11465 176-178 CD denotes 30
T11466 179-181 NN denotes µl
T11467 182-192 VBG denotes containing
T11468 193-196 CD denotes 0.5
T11469 197-199 NN denotes µg
T11470 200-202 IN denotes of
T11471 203-208 JJ denotes total
T11472 209-212 NNP denotes RNA
T11473 212-213 . denotes .
T11474 214-219 NNS denotes dNTPs
T11475 219-220 , denotes ,
T11476 221-226 NN denotes oligo
T11477 226-227 -LRB- denotes (
T11478 227-229 NN denotes dT
T11479 229-230 -RRB- denotes )
T11480 230-232 CD denotes 15
T11481 233-239 NN denotes primer
T11482 239-240 , denotes ,
T11483 241-252 JJ denotes recombinant
T11484 253-259 NNP denotes RNasin
T11485 260-272 NNP denotes Ribonuclease
T11486 273-282 NNP denotes Inhibitor
T11487 282-283 , denotes ,
T11488 284-287 CC denotes and
T11489 288-293 NNP denotes M-MLV
T11490 294-301 VB denotes Reverse
T11491 302-315 NNP denotes Transcriptase
T11492 316-317 -LRB- denotes (
T11493 317-320 DT denotes all
T11494 321-325 IN denotes from
T11495 326-333 NNP denotes Promega
T11496 333-334 , denotes ,
T11497 335-342 NNP denotes Madison
T11498 342-343 , denotes ,
T11499 344-346 NNP denotes WI
T11500 346-347 -RRB- denotes )
T11501 348-352 VBD denotes were
T11502 353-357 VBN denotes used
T11503 358-367 VBG denotes according
T11504 368-370 TO denotes to
T11505 371-374 DT denotes the
T11506 375-387 NN denotes manufacturer
T11507 387-389 POS denotes 's
T11508 390-401 NN denotes instruction
T11509 401-402 . denotes .
R9470 T11432 T11436 nsubjpass Primers,purchased
R9471 T11433 T11432 cc and,Primers
R9472 T11434 T11432 conj probes,Primers
R9473 T11435 T11436 auxpass were,purchased
R9474 T11436 T11436 ROOT purchased,purchased
R9475 T11437 T11436 prep from,purchased
R9476 T11438 T11439 compound Applied,Biosystems
R9477 T11439 T11437 pobj Biosystems,from
R9478 T11440 T11436 punct .,purchased
R9479 T11441 T11442 amod Reverse,transcription
R9480 T11442 T11444 nsubjpass transcription,performed
R9481 T11443 T11444 auxpass was,performed
R9482 T11444 T11444 ROOT performed,performed
R9483 T11445 T11444 prep at,performed
R9484 T11446 T11447 nummod 42,°
R9485 T11447 T11445 pobj °,at
R9486 T11448 T11444 npadvmod C,performed
R9487 T11449 T11444 prep for,performed
R9488 T11450 T11451 nummod 60,minutes
R9489 T11451 T11449 pobj minutes,for
R9490 T11452 T11451 prep with,minutes
R9491 T11453 T11456 det the,step
R9492 T11454 T11456 amod final,step
R9493 T11455 T11456 compound denaturation,step
R9494 T11456 T11452 pobj step,with
R9495 T11457 T11456 prep at,step
R9496 T11458 T11459 nummod 90,°
R9497 T11459 T11457 pobj °,at
R9498 T11460 T11461 compound C,for
R9499 T11461 T11444 prep for,performed
R9500 T11462 T11463 nummod 5,minutes
R9501 T11463 T11461 pobj minutes,for
R9502 T11464 T11463 prep in,minutes
R9503 T11465 T11466 nummod 30,µl
R9504 T11466 T11464 pobj µl,in
R9505 T11467 T11466 acl containing,µl
R9506 T11468 T11469 nummod 0.5,µg
R9507 T11469 T11467 dobj µg,containing
R9508 T11470 T11469 prep of,µg
R9509 T11471 T11472 amod total,RNA
R9510 T11472 T11470 pobj RNA,of
R9511 T11473 T11444 punct .,performed
R9512 T11474 T11502 nsubjpass dNTPs,used
R9513 T11475 T11474 punct ",",dNTPs
R9514 T11476 T11474 conj oligo,dNTPs
R9515 T11477 T11478 punct (,dT
R9516 T11478 T11481 nmod dT,primer
R9517 T11479 T11478 punct ),dT
R9518 T11480 T11481 nummod 15,primer
R9519 T11481 T11474 appos primer,dNTPs
R9520 T11482 T11481 punct ",",primer
R9521 T11483 T11486 amod recombinant,Inhibitor
R9522 T11484 T11486 compound RNasin,Inhibitor
R9523 T11485 T11486 compound Ribonuclease,Inhibitor
R9524 T11486 T11481 appos Inhibitor,primer
R9525 T11487 T11481 punct ",",primer
R9526 T11488 T11481 cc and,primer
R9527 T11489 T11490 nsubj M-MLV,Reverse
R9528 T11490 T11502 advmod Reverse,used
R9529 T11491 T11490 npadvmod Transcriptase,Reverse
R9530 T11492 T11493 punct (,all
R9531 T11493 T11494 advmod all,from
R9532 T11494 T11490 prep from,Reverse
R9533 T11495 T11494 pobj Promega,from
R9534 T11496 T11495 punct ",",Promega
R9535 T11497 T11495 conj Madison,Promega
R9536 T11498 T11497 punct ",",Madison
R9537 T11499 T11497 conj WI,Madison
R9538 T11500 T11490 punct ),Reverse
R9539 T11501 T11502 auxpass were,used
R9540 T11502 T11502 ROOT used,used
R9541 T11503 T11502 prep according,used
R9542 T11504 T11503 prep to,according
R9543 T11505 T11506 det the,manufacturer
R9544 T11506 T11508 poss manufacturer,instruction
R9545 T11507 T11506 case 's,manufacturer
R9546 T11508 T11504 pobj instruction,to
R9547 T11509 T11502 punct .,used

GO-BP

Id Subject Object Predicate Lexical cue
T11843 59-80 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T11845 67-80 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T11847 302-315 http://purl.obolibrary.org/obo/GO_0003899 denotes Transcriptase
T11848 302-315 http://purl.obolibrary.org/obo/GO_0003968 denotes Transcriptase
T11849 302-315 http://purl.obolibrary.org/obo/GO_0034062 denotes Transcriptase

GO-MF

Id Subject Object Predicate Lexical cue
T11853 302-315 http://purl.obolibrary.org/obo/GO_0003899 denotes Transcriptase
T11854 302-315 http://purl.obolibrary.org/obo/GO_0003968 denotes Transcriptase
T11855 302-315 http://purl.obolibrary.org/obo/GO_0034062 denotes Transcriptase

sentences

Id Subject Object Predicate Lexical cue
T11354 0-402 Sentence denotes Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.
T215 0-58 Sentence denotes Primers and probes were purchased from Applied Biosystems.
T216 59-402 Sentence denotes Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.

events-check-again

Id Subject Object Predicate Lexical cue
T11898 288-315 Protein denotes M-MLV Reverse Transcriptase

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T11913 253-272 Protein denotes RNasin Ribonuclease
T11914 288-315 Protein denotes M-MLV Reverse Transcriptase

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T11343 288-315 Protein denotes M-MLV Reverse Transcriptase

test2

Id Subject Object Predicate Lexical cue
T11327 288-315 Protein denotes M-MLV Reverse Transcriptase