PMC:3279418 / 11346-12341 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3279418","sourcedb":"PMC","sourceid":"3279418","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3279418","text":"10.1371/journal.pone.0031809.g003 Figure 3 Defective production of pro-inflammatory mediators from stimulated cpdm BMDC.\n(A–D) Cultured WT and cpdm BMDC (1×105 cells in 0.1 ml complete medium) were washed and stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours. Supernatants were collected for ELISA of IL12P70, IL6, and GMCSF, and for quantification of nitric oxide (NO). (E–F) Gradient numbers (1×104, 2×104, and 4×104 cells in 0.1 mL complete medium) of BMDC were used for100 ng/mL LPS stimulation. After 24 hours, the amounts of IL12P70 and IL6 from the supernatant were measured. (G) The cpdm mice rescued by Sharpin-containing BAC had complete remission of the inflammatory phenotype [2]. BMDC developed from cpdm and rescued cpdm mice were plated (1×106 cells in 0.3 mL) and stimulated with 100 ng/mL LPS. After 24 hours, supernatants were collected for analysis of IL12P70 production. 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