PMC:3245220 / 8087-10511 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5134","span":{"begin":0,"end":5},"obj":"P09603"},{"id":"T5135","span":{"begin":2,"end":5},"obj":"P04141"},{"id":"T5136","span":{"begin":148,"end":153},"obj":"P09603"},{"id":"T5137","span":{"begin":150,"end":153},"obj":"P04141"},{"id":"T5138","span":{"begin":253,"end":258},"obj":"P09603"},{"id":"T5139","span":{"begin":255,"end":258},"obj":"P04141"},{"id":"T5140","span":{"begin":409,"end":414},"obj":"P09603"},{"id":"T5141","span":{"begin":411,"end":414},"obj":"P04141"},{"id":"T5142","span":{"begin":590,"end":595},"obj":"P09603"},{"id":"T5143","span":{"begin":592,"end":595},"obj":"P04141"},{"id":"T5144","span":{"begin":938,"end":943},"obj":"P09603"},{"id":"T5145","span":{"begin":940,"end":943},"obj":"P04141"},{"id":"T5146","span":{"begin":2016,"end":2021},"obj":"P09603"},{"id":"T5147","span":{"begin":2018,"end":2021},"obj":"P04141"},{"id":"T5148","span":{"begin":2215,"end":2220},"obj":"P09603"},{"id":"T5149","span":{"begin":2217,"end":2220},"obj":"P04141"},{"id":"T5150","span":{"begin":2364,"end":2369},"obj":"P09603"},{"id":"T5151","span":{"begin":2366,"end":2369},"obj":"P04141"},{"id":"T23146","span":{"begin":1001,"end":1006},"obj":"P09603"},{"id":"T23147","span":{"begin":1003,"end":1006},"obj":"P04141"},{"id":"T23148","span":{"begin":1128,"end":1133},"obj":"P09603"},{"id":"T23149","span":{"begin":1130,"end":1133},"obj":"P04141"},{"id":"T23150","span":{"begin":1389,"end":1394},"obj":"P09603"},{"id":"T23151","span":{"begin":1391,"end":1394},"obj":"P04141"},{"id":"T23152","span":{"begin":1842,"end":1847},"obj":"P09603"},{"id":"T23153","span":{"begin":1844,"end":1847},"obj":"P04141"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T5152","span":{"begin":2250,"end":2259},"obj":"Positive_regulation"},{"id":"T5153","span":{"begin":2275,"end":2283},"obj":"Positive_regulation"},{"id":"T5154","span":{"begin":2260,"end":2265},"obj":"Protein"},{"id":"T5155","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T5156","span":{"begin":148,"end":153},"obj":"Protein"},{"id":"T5157","span":{"begin":253,"end":258},"obj":"Protein"},{"id":"T5158","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T5159","span":{"begin":590,"end":595},"obj":"Protein"},{"id":"T5160","span":{"begin":938,"end":943},"obj":"Protein"},{"id":"T5161","span":{"begin":2016,"end":2021},"obj":"Protein"},{"id":"T5162","span":{"begin":2215,"end":2220},"obj":"Protein"},{"id":"T5163","span":{"begin":2364,"end":2369},"obj":"Protein"},{"id":"T23154","span":{"begin":1001,"end":1006},"obj":"Protein"},{"id":"T23155","span":{"begin":1128,"end":1133},"obj":"Protein"},{"id":"T23156","span":{"begin":1389,"end":1394},"obj":"Protein"},{"id":"T23157","span":{"begin":1842,"end":1847},"obj":"Protein"}],"relations":[{"id":"R3885","pred":"themeOf","subj":"T5153","obj":"T5152"},{"id":"R3886","pred":"themeOf","subj":"T5154","obj":"T5153"},{"id":"R3887","pred":"causeOf","subj":"T5162","obj":"T5152"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T5264","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T5265","span":{"begin":14,"end":19},"obj":"Protein"},{"id":"T5266","span":{"begin":148,"end":153},"obj":"Protein"},{"id":"T5267","span":{"begin":162,"end":171},"obj":"Protein"},{"id":"T5268","span":{"begin":253,"end":258},"obj":"Protein"},{"id":"T5269","span":{"begin":172,"end":179},"obj":"Binding"},{"id":"T5270","span":{"begin":154,"end":161},"obj":"Positive_regulation"},{"id":"T5271","span":{"begin":307,"end":320},"obj":"Protein"},{"id":"T5272","span":{"begin":336,"end":341},"obj":"Binding"},{"id":"T5273","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T5274","span":{"begin":429,"end":434},"obj":"Protein"},{"id":"T5275","span":{"begin":439,"end":446},"obj":"Binding"},{"id":"T5276","span":{"begin":423,"end":428},"obj":"Regulation"},{"id":"T5277","span":{"begin":516,"end":538},"obj":"Protein"},{"id":"T5278","span":{"begin":555,"end":560},"obj":"Protein"},{"id":"T5279","span":{"begin":590,"end":595},"obj":"Protein"},{"id":"T5280","span":{"begin":666,"end":678},"obj":"Protein"},{"id":"T5281","span":{"begin":487,"end":499},"obj":"Gene_expression"},{"id":"T5282","span":{"begin":654,"end":662},"obj":"Positive_regulation"},{"id":"T5283","span":{"begin":782,"end":786},"obj":"Protein"},{"id":"T5284","span":{"begin":787,"end":791},"obj":"Protein"},{"id":"T5285","span":{"begin":810,"end":829},"obj":"Protein"},{"id":"T5286","span":{"begin":802,"end":809},"obj":"Negative_regulation"},{"id":"T5287","span":{"begin":867,"end":886},"obj":"Protein"},{"id":"T5288","span":{"begin":903,"end":907},"obj":"Protein"},{"id":"T5289","span":{"begin":938,"end":943},"obj":"Protein"},{"id":"T5290","span":{"begin":846,"end":857},"obj":"Gene_expression"},{"id":"T5291","span":{"begin":846,"end":857},"obj":"Positive_regulation"},{"id":"T5292","span":{"begin":895,"end":902},"obj":"Gene_expression"},{"id":"T5293","span":{"begin":2016,"end":2021},"obj":"Protein"},{"id":"T5294","span":{"begin":2030,"end":2035},"obj":"Protein"},{"id":"T5295","span":{"begin":2022,"end":2029},"obj":"Positive_regulation"},{"id":"T5296","span":{"begin":2140,"end":2145},"obj":"Protein"},{"id":"T5297","span":{"begin":2146,"end":2150},"obj":"Protein"},{"id":"T5298","span":{"begin":2260,"end":2274},"obj":"Protein"},{"id":"T5299","span":{"begin":2250,"end":2259},"obj":"Positive_regulation"},{"id":"T5300","span":{"begin":2364,"end":2369},"obj":"Protein"},{"id":"T5301","span":{"begin":2378,"end":2383},"obj":"Protein"},{"id":"T23195","span":{"begin":1001,"end":1006},"obj":"Protein"},{"id":"T23196","span":{"begin":1015,"end":1020},"obj":"Protein"},{"id":"T23197","span":{"begin":1007,"end":1014},"obj":"Positive_regulation"},{"id":"T23198","span":{"begin":1128,"end":1133},"obj":"Protein"},{"id":"T23199","span":{"begin":1156,"end":1161},"obj":"Protein"},{"id":"T23200","span":{"begin":1166,"end":1173},"obj":"Binding"},{"id":"T23201","span":{"begin":1348,"end":1370},"obj":"Protein"},{"id":"T23202","span":{"begin":1389,"end":1394},"obj":"Protein"},{"id":"T23203","span":{"begin":1471,"end":1476},"obj":"Protein"},{"id":"T23204","span":{"begin":1526,"end":1530},"obj":"Protein"},{"id":"T23205","span":{"begin":1599,"end":1603},"obj":"Protein"},{"id":"T23206","span":{"begin":1531,"end":1539},"obj":"Localization"},{"id":"T23207","span":{"begin":1531,"end":1539},"obj":"Localization"},{"id":"T23208","span":{"begin":1587,"end":1595},"obj":"Positive_regulation"},{"id":"T23209","span":{"begin":1730,"end":1733},"obj":"Protein"},{"id":"T23210","span":{"begin":1734,"end":1736},"obj":"Protein"},{"id":"T23211","span":{"begin":1737,"end":1741},"obj":"Protein"},{"id":"T23212","span":{"begin":1824,"end":1847},"obj":"Protein"}],"relations":[{"id":"R3907","pred":"causeOf","subj":"T5266","obj":"T5270"},{"id":"R3908","pred":"themeOf","subj":"T5267","obj":"T5269"},{"id":"R3909","pred":"themeOf","subj":"T5269","obj":"T5270"},{"id":"R3910","pred":"themeOf","subj":"T5271","obj":"T5272"},{"id":"R3911","pred":"causeOf","subj":"T5273","obj":"T5276"},{"id":"R3912","pred":"themeOf","subj":"T5274","obj":"T5275"},{"id":"R3913","pred":"themeOf","subj":"T5275","obj":"T5276"},{"id":"R3914","pred":"themeOf","subj":"T5277","obj":"T5281"},{"id":"R3915","pred":"themeOf","subj":"T5280","obj":"T5282"},{"id":"R3916","pred":"themeOf","subj":"T5285","obj":"T5286"},{"id":"R3917","pred":"themeOf","subj":"T5287","obj":"T5290"},{"id":"R3918","pred":"themeOf","subj":"T5288","obj":"T5292"},{"id":"R3919","pred":"themeOf","subj":"T5290","obj":"T5291"},{"id":"R3920","pred":"causeOf","subj":"T5293","obj":"T5295"},{"id":"R3921","pred":"themeOf","subj":"T5294","obj":"T5295"},{"id":"R3922","pred":"themeOf","subj":"T5298","obj":"T5299"},{"id":"R17695","pred":"causeOf","subj":"T23195","obj":"T23197"},{"id":"R17696","pred":"themeOf","subj":"T23196","obj":"T23197"},{"id":"R17697","pred":"themeOf","subj":"T23199","obj":"T23200"},{"id":"R17698","pred":"themeOf","subj":"T23203","obj":"T23206"},{"id":"R17699","pred":"themeOf","subj":"T23204","obj":"T23207"},{"id":"R17700","pred":"themeOf","subj":"T23205","obj":"T23208"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"events-check-again","denotations":[{"id":"T5252","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T5253","span":{"begin":148,"end":153},"obj":"Protein"},{"id":"T5254","span":{"begin":253,"end":258},"obj":"Protein"},{"id":"T5255","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T5256","span":{"begin":590,"end":595},"obj":"Protein"},{"id":"T5257","span":{"begin":938,"end":943},"obj":"Protein"},{"id":"T5258","span":{"begin":2016,"end":2021},"obj":"Protein"},{"id":"T5259","span":{"begin":2215,"end":2220},"obj":"Protein"},{"id":"T5260","span":{"begin":2250,"end":2259},"obj":"Positive_regulation"},{"id":"T5261","span":{"begin":2260,"end":2265},"obj":"Protein"},{"id":"T5262","span":{"begin":2275,"end":2283},"obj":"Positive_regulation"},{"id":"T5263","span":{"begin":2364,"end":2369},"obj":"Protein"},{"id":"T23191","span":{"begin":1001,"end":1006},"obj":"Protein"},{"id":"T23192","span":{"begin":1128,"end":1133},"obj":"Protein"},{"id":"T23193","span":{"begin":1389,"end":1394},"obj":"Protein"},{"id":"T23194","span":{"begin":1842,"end":1847},"obj":"Protein"}],"relations":[{"id":"R3904","pred":"causeOf","subj":"T5259","obj":"T5260"},{"id":"R3905","pred":"themeOf","subj":"T5261","obj":"T5262"},{"id":"R3906","pred":"themeOf","subj":"T5262","obj":"T5260"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"GO-CC","denotations":[{"id":"T5184","span":{"begin":111,"end":115},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5185","span":{"begin":625,"end":630},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5186","span":{"begin":2101,"end":2106},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5187","span":{"begin":2244,"end":2249},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5188","span":{"begin":2321,"end":2326},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T23169","span":{"begin":1656,"end":1661},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T23170","span":{"begin":1677,"end":1682},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"GO-MF","denotations":[{"id":"T5176","span":{"begin":168,"end":179},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T5177","span":{"begin":435,"end":446},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T5178","span":{"begin":172,"end":179},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5179","span":{"begin":439,"end":446},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5180","span":{"begin":571,"end":578},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5181","span":{"begin":816,"end":823},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T5182","span":{"begin":810,"end":823},"obj":"http://purl.obolibrary.org/obo/GO_0051059"},{"id":"T5183","span":{"begin":2022,"end":2044},"obj":"http://purl.obolibrary.org/obo/GO_0004704"},{"id":"T23167","span":{"begin":1162,"end":1173},"obj":"http://purl.obolibrary.org/obo/GO_0003677"},{"id":"T23168","span":{"begin":1166,"end":1173},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"GO-BP","denotations":[{"id":"T5174","span":{"begin":2022,"end":2044},"obj":"http://purl.obolibrary.org/obo/GO_0004704"},{"id":"T5175","span":{"begin":2384,"end":2399},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T23166","span":{"begin":1021,"end":1036},"obj":"http://purl.obolibrary.org/obo/GO_0006351"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"UBERON-AE","denotations":[{"id":"T22809","span":{"begin":1764,"end":1769},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"pmc-enju-pas","denotations":[{"id":"T4910","span":{"begin":0,"end":5},"obj":"NN"},{"id":"T4911","span":{"begin":6,"end":13},"obj":"VB"},{"id":"T4912","span":{"begin":14,"end":19},"obj":"NN"},{"id":"T4913","span":{"begin":20,"end":35},"obj":"JJ"},{"id":"T4914","span":{"begin":36,"end":44},"obj":"NN"},{"id":"T4915","span":{"begin":45,"end":47},"obj":"IN"},{"id":"T4916","span":{"begin":48,"end":53},"obj":"JJ"},{"id":"T4917","span":{"begin":54,"end":70},"obj":"NNP"},{"id":"T4918","span":{"begin":71,"end":82},"obj":"NN"},{"id":"T4919","span":{"begin":83,"end":84},"obj":"-LRB-"},{"id":"T4920","span":{"begin":84,"end":88},"obj":"NN"},{"id":"T4921","span":{"begin":88,"end":89},"obj":"-RRB-"},{"id":"T4922","span":{"begin":90,"end":93},"obj":"CC"},{"id":"T4923","span":{"begin":94,"end":99},"obj":"NNP"},{"id":"T4924","span":{"begin":100,"end":110},"obj":"NNP"},{"id":"T4925","span":{"begin":111,"end":115},"obj":"NNP"},{"id":"T4926","span":{"begin":116,"end":120},"obj":"NNP"},{"id":"T4927","span":{"begin":120,"end":121},"obj":"-COMMA-"},{"id":"T4928","span":{"begin":122,"end":125},"obj":"NN"},{"id":"T4929","span":{"begin":126,"end":131},"obj":"CD"},{"id":"T4930","span":{"begin":132,"end":134},"obj":"TO"},{"id":"T4931","span":{"begin":135,"end":144},"obj":"VB"},{"id":"T4932","span":{"begin":145,"end":147},"obj":"IN"},{"id":"T4933","span":{"begin":148,"end":153},"obj":"NN"},{"id":"T4934","span":{"begin":154,"end":161},"obj":"VB"},{"id":"T4935","span":{"begin":162,"end":167},"obj":"NN"},{"id":"T4936","span":{"begin":168,"end":171},"obj":"NN"},{"id":"T4937","span":{"begin":172,"end":179},"obj":"NN"},{"id":"T4938","span":{"begin":180,"end":182},"obj":"IN"},{"id":"T4939","span":{"begin":183,"end":188},"obj":"JJ"},{"id":"T4940","span":{"begin":189,"end":200},"obj":"NN"},{"id":"T4941","span":{"begin":200,"end":201},"obj":"-COMMA-"},{"id":"T4942","span":{"begin":202,"end":204},"obj":"PRP"},{"id":"T4943","span":{"begin":205,"end":214},"obj":"VB"},{"id":"T4944","span":{"begin":215,"end":219},"obj":"NN"},{"id":"T4945","span":{"begin":220,"end":228},"obj":"NN"},{"id":"T4946","span":{"begin":229,"end":231},"obj":"IN"},{"id":"T4947","span":{"begin":232,"end":239},"obj":"JJ"},{"id":"T4948","span":{"begin":240,"end":247},"obj":"NN"},{"id":"T4949","span":{"begin":248,"end":252},"obj":"IN"},{"id":"T4950","span":{"begin":253,"end":266},"obj":"JJ"},{"id":"T4951","span":{"begin":267,"end":272},"obj":"NN"},{"id":"T4952","span":{"begin":273,"end":280},"obj":"JJ"},{"id":"T4953","span":{"begin":281,"end":283},"obj":"TO"},{"id":"T4954","span":{"begin":284,"end":292},"obj":"JJ"},{"id":"T4955","span":{"begin":293,"end":300},"obj":"NN"},{"id":"T4956","span":{"begin":301,"end":302},"obj":"-LRB-"},{"id":"T4957","span":{"begin":302,"end":304},"obj":"CD"},{"id":"T4958","span":{"begin":304,"end":305},"obj":"-RRB-"},{"id":"T4959","span":{"begin":305,"end":306},"obj":"-COMMA-"},{"id":"T4960","span":{"begin":307,"end":314},"obj":"JJ"},{"id":"T4961","span":{"begin":315,"end":320},"obj":"NN"},{"id":"T4962","span":{"begin":321,"end":335},"obj":"RB"},{"id":"T4963","span":{"begin":336,"end":341},"obj":"VB"},{"id":"T4964","span":{"begin":342,"end":345},"obj":"NN"},{"id":"T4965","span":{"begin":346,"end":348},"obj":"IN"},{"id":"T4966","span":{"begin":349,"end":363},"obj":"JJ"},{"id":"T4967","span":{"begin":364,"end":373},"obj":"NN"},{"id":"T4968","span":{"begin":374,"end":375},"obj":"-LRB-"},{"id":"T4969","span":{"begin":375,"end":381},"obj":"NN"},{"id":"T4970","span":{"begin":382,"end":384},"obj":"NN"},{"id":"T4971","span":{"begin":384,"end":385},"obj":"-RRB-"},{"id":"T4972","span":{"begin":387,"end":400},"obj":"RB"},{"id":"T4973","span":{"begin":400,"end":401},"obj":"-COMMA-"},{"id":"T4974","span":{"begin":402,"end":408},"obj":"VB"},{"id":"T4975","span":{"begin":409,"end":414},"obj":"NN"},{"id":"T4976","span":{"begin":415,"end":418},"obj":"VB"},{"id":"T4977","span":{"begin":419,"end":422},"obj":"RB"},{"id":"T4978","span":{"begin":423,"end":428},"obj":"VB"},{"id":"T4979","span":{"begin":429,"end":434},"obj":"NN"},{"id":"T4980","span":{"begin":435,"end":438},"obj":"NN"},{"id":"T4981","span":{"begin":439,"end":446},"obj":"NN"},{"id":"T4982","span":{"begin":447,"end":449},"obj":"IN"},{"id":"T4983","span":{"begin":450,"end":454},"obj":"NN"},{"id":"T4984","span":{"begin":456,"end":458},"obj":"IN"},{"id":"T4985","span":{"begin":459,"end":467},"obj":"NN"},{"id":"T4986","span":{"begin":467,"end":468},"obj":"-COMMA-"},{"id":"T4987","span":{"begin":469,"end":474},"obj":"IN"},{"id":"T4988","span":{"begin":475,"end":486},"obj":"RB"},{"id":"T4989","span":{"begin":487,"end":499},"obj":"VB"},{"id":"T4990","span":{"begin":500,"end":505},"obj":"JJ"},{"id":"T4991","span":{"begin":506,"end":510},"obj":"NN"},{"id":"T4992","span":{"begin":511,"end":515},"obj":"IN"},{"id":"T4993","span":{"begin":516,"end":527},"obj":"NN"},{"id":"T4994","span":{"begin":528,"end":538},"obj":"NN"},{"id":"T4995","span":{"begin":539,"end":549},"obj":"VB"},{"id":"T4996","span":{"begin":550,"end":554},"obj":"CD"},{"id":"T4997","span":{"begin":555,"end":560},"obj":"NN"},{"id":"T4998","span":{"begin":561,"end":570},"obj":"NN"},{"id":"T4999","span":{"begin":571,"end":578},"obj":"NN"},{"id":"T5000","span":{"begin":579,"end":588},"obj":"NN"},{"id":"T5001","span":{"begin":588,"end":589},"obj":"-COMMA-"},{"id":"T5002","span":{"begin":590,"end":595},"obj":"NN"},{"id":"T5003","span":{"begin":596,"end":605},"obj":"NN"},{"id":"T5004","span":{"begin":606,"end":608},"obj":"IN"},{"id":"T5005","span":{"begin":609,"end":612},"obj":"DT"},{"id":"T5006","span":{"begin":613,"end":624},"obj":"VB"},{"id":"T5007","span":{"begin":625,"end":630},"obj":"NN"},{"id":"T5008","span":{"begin":631,"end":639},"obj":"VB"},{"id":"T5009","span":{"begin":640,"end":642},"obj":"IN"},{"id":"T5010","span":{"begin":643,"end":644},"obj":"DT"},{"id":"T5011","span":{"begin":645,"end":653},"obj":"JJ"},{"id":"T5012","span":{"begin":654,"end":662},"obj":"NN"},{"id":"T5013","span":{"begin":663,"end":665},"obj":"IN"},{"id":"T5014","span":{"begin":666,"end":670},"obj":"NN"},{"id":"T5015","span":{"begin":671,"end":678},"obj":"NN"},{"id":"T5016","span":{"begin":679,"end":681},"obj":"IN"},{"id":"T5017","span":{"begin":682,"end":685},"obj":"DT"},{"id":"T5018","span":{"begin":686,"end":693},"obj":"NN"},{"id":"T5019","span":{"begin":694,"end":699},"obj":"NN"},{"id":"T5020","span":{"begin":700,"end":708},"obj":"VB"},{"id":"T5021","span":{"begin":709,"end":711},"obj":"TO"},{"id":"T5022","span":{"begin":712,"end":715},"obj":"NNP"},{"id":"T5023","span":{"begin":716,"end":717},"obj":"-LRB-"},{"id":"T5024","span":{"begin":717,"end":724},"obj":"NN"},{"id":"T5025","span":{"begin":724,"end":725},"obj":"-RRB-"},{"id":"T5026","span":{"begin":725,"end":733},"obj":"JJ"},{"id":"T5027","span":{"begin":734,"end":745},"obj":"VB"},{"id":"T5028","span":{"begin":746,"end":750},"obj":"NN"},{"id":"T5029","span":{"begin":751,"end":752},"obj":"-LRB-"},{"id":"T5030","span":{"begin":752,"end":758},"obj":"NN"},{"id":"T5031","span":{"begin":759,"end":761},"obj":"NN"},{"id":"T5032","span":{"begin":761,"end":762},"obj":"-RRB-"},{"id":"T5033","span":{"begin":764,"end":766},"obj":"IN"},{"id":"T5034","span":{"begin":767,"end":768},"obj":"DT"},{"id":"T5035","span":{"begin":769,"end":776},"obj":"NN"},{"id":"T5036","span":{"begin":776,"end":777},"obj":"-COMMA-"},{"id":"T5037","span":{"begin":778,"end":781},"obj":"DT"},{"id":"T5038","span":{"begin":782,"end":791},"obj":"NN"},{"id":"T5039","span":{"begin":792,"end":801},"obj":"NN"},{"id":"T5040","span":{"begin":802,"end":809},"obj":"VB"},{"id":"T5041","span":{"begin":810,"end":815},"obj":"NN"},{"id":"T5042","span":{"begin":816,"end":823},"obj":"NN"},{"id":"T5043","span":{"begin":824,"end":829},"obj":"NN"},{"id":"T5044","span":{"begin":830,"end":833},"obj":"VB"},{"id":"T5045","span":{"begin":834,"end":838},"obj":"VB"},{"id":"T5046","span":{"begin":840,"end":845},"obj":"NN"},{"id":"T5047","span":{"begin":846,"end":857},"obj":"VB"},{"id":"T5048","span":{"begin":858,"end":862},"obj":"IN"},{"id":"T5049","span":{"begin":863,"end":866},"obj":"DT"},{"id":"T5050","span":{"begin":867,"end":876},"obj":"NN"},{"id":"T5051","span":{"begin":877,"end":886},"obj":"NN"},{"id":"T5052","span":{"begin":887,"end":890},"obj":"VB"},{"id":"T5053","span":{"begin":891,"end":894},"obj":"RB"},{"id":"T5054","span":{"begin":895,"end":902},"obj":"VB"},{"id":"T5055","span":{"begin":903,"end":907},"obj":"NN"},{"id":"T5056","span":{"begin":908,"end":910},"obj":"IN"},{"id":"T5057","span":{"begin":911,"end":914},"obj":"DT"},{"id":"T5058","span":{"begin":915,"end":922},"obj":"NN"},{"id":"T5059","span":{"begin":923,"end":925},"obj":"CC"},{"id":"T5060","span":{"begin":926,"end":934},"obj":"NN"},{"id":"T5061","span":{"begin":935,"end":937},"obj":"IN"},{"id":"T5062","span":{"begin":938,"end":943},"obj":"NN"},{"id":"T5063","span":{"begin":944,"end":945},"obj":"-LRB-"},{"id":"T5064","span":{"begin":945,"end":951},"obj":"NN"},{"id":"T5065","span":{"begin":952,"end":954},"obj":"NN"},{"id":"T5066","span":{"begin":954,"end":955},"obj":"-RRB-"},{"id":"T5067","span":{"begin":1987,"end":1989},"obj":"PRP"},{"id":"T5068","span":{"begin":1990,"end":1994},"obj":"RB"},{"id":"T5069","span":{"begin":1995,"end":2007},"obj":"VB"},{"id":"T5070","span":{"begin":2008,"end":2015},"obj":"IN"},{"id":"T5071","span":{"begin":2016,"end":2021},"obj":"NN"},{"id":"T5072","span":{"begin":2022,"end":2029},"obj":"VB"},{"id":"T5073","span":{"begin":2030,"end":2035},"obj":"NN"},{"id":"T5074","span":{"begin":2036,"end":2044},"obj":"NN"},{"id":"T5075","span":{"begin":2045,"end":2047},"obj":"IN"},{"id":"T5076","span":{"begin":2048,"end":2051},"obj":"DT"},{"id":"T5077","span":{"begin":2052,"end":2057},"obj":"NN"},{"id":"T5078","span":{"begin":2058,"end":2068},"obj":"NN"},{"id":"T5079","span":{"begin":2069,"end":2073},"obj":"NN"},{"id":"T5080","span":{"begin":2074,"end":2078},"obj":"NN"},{"id":"T5081","span":{"begin":2078,"end":2079},"obj":"-COMMA-"},{"id":"T5082","span":{"begin":2080,"end":2083},"obj":"NN"},{"id":"T5083","span":{"begin":2084,"end":2089},"obj":"CD"},{"id":"T5084","span":{"begin":2091,"end":2094},"obj":"NN"},{"id":"T5085","span":{"begin":2095,"end":2100},"obj":"CD"},{"id":"T5086","span":{"begin":2101,"end":2106},"obj":"NN"},{"id":"T5087","span":{"begin":2107,"end":2111},"obj":"VB"},{"id":"T5088","span":{"begin":2112,"end":2123},"obj":"VB"},{"id":"T5089","span":{"begin":2124,"end":2128},"obj":"IN"},{"id":"T5090","span":{"begin":2129,"end":2135},"obj":"CC"},{"id":"T5091","span":{"begin":2136,"end":2139},"obj":"DT"},{"id":"T5092","span":{"begin":2140,"end":2150},"obj":"NN"},{"id":"T5093","span":{"begin":2151,"end":2159},"obj":"NN"},{"id":"T5094","span":{"begin":2160,"end":2162},"obj":"CC"},{"id":"T5095","span":{"begin":2163,"end":2170},"obj":"NN"},{"id":"T5096","span":{"begin":2171,"end":2180},"obj":"NN"},{"id":"T5097","span":{"begin":2181,"end":2190},"obj":"NN"},{"id":"T5098","span":{"begin":2192,"end":2194},"obj":"IN"},{"id":"T5099","span":{"begin":2195,"end":2200},"obj":"VB"},{"id":"T5100","span":{"begin":2201,"end":2203},"obj":"IN"},{"id":"T5101","span":{"begin":2204,"end":2210},"obj":"NN"},{"id":"T5102","span":{"begin":2211,"end":2213},"obj":"NN"},{"id":"T5103","span":{"begin":2213,"end":2214},"obj":"-COMMA-"},{"id":"T5104","span":{"begin":2215,"end":2220},"obj":"NN"},{"id":"T5105","span":{"begin":2221,"end":2230},"obj":"NN"},{"id":"T5106","span":{"begin":2231,"end":2233},"obj":"IN"},{"id":"T5107","span":{"begin":2234,"end":2237},"obj":"NN"},{"id":"T5108","span":{"begin":2238,"end":2243},"obj":"CD"},{"id":"T5109","span":{"begin":2244,"end":2249},"obj":"NN"},{"id":"T5110","span":{"begin":2250,"end":2259},"obj":"VB"},{"id":"T5111","span":{"begin":2260,"end":2265},"obj":"NN"},{"id":"T5112","span":{"begin":2266,"end":2274},"obj":"NN"},{"id":"T5113","span":{"begin":2275,"end":2283},"obj":"NN"},{"id":"T5114","span":{"begin":2284,"end":2286},"obj":"IN"},{"id":"T5115","span":{"begin":2287,"end":2295},"obj":"RB"},{"id":"T5116","span":{"begin":2296,"end":2300},"obj":"IN"},{"id":"T5117","span":{"begin":2301,"end":2305},"obj":"DT"},{"id":"T5118","span":{"begin":2306,"end":2308},"obj":"IN"},{"id":"T5119","span":{"begin":2309,"end":2320},"obj":"JJ"},{"id":"T5120","span":{"begin":2321,"end":2326},"obj":"NN"},{"id":"T5121","span":{"begin":2328,"end":2336},"obj":"RB"},{"id":"T5122","span":{"begin":2336,"end":2337},"obj":"-COMMA-"},{"id":"T5123","span":{"begin":2338,"end":2341},"obj":"PRP-DOLLAR-"},{"id":"T5124","span":{"begin":2342,"end":2346},"obj":"NN"},{"id":"T5125","span":{"begin":2347,"end":2358},"obj":"VB"},{"id":"T5126","span":{"begin":2359,"end":2363},"obj":"IN"},{"id":"T5127","span":{"begin":2364,"end":2369},"obj":"NN"},{"id":"T5128","span":{"begin":2370,"end":2377},"obj":"VB"},{"id":"T5129","span":{"begin":2378,"end":2383},"obj":"NN"},{"id":"T5130","span":{"begin":2384,"end":2399},"obj":"JJ"},{"id":"T5131","span":{"begin":2400,"end":2408},"obj":"NN"},{"id":"T5132","span":{"begin":2409,"end":2411},"obj":"IN"},{"id":"T5133","span":{"begin":2412,"end":2423},"obj":"NN"},{"id":"T22987","span":{"begin":1001,"end":1006},"obj":"NN"},{"id":"T22988","span":{"begin":1007,"end":1014},"obj":"VB"},{"id":"T22989","span":{"begin":1015,"end":1020},"obj":"NN"},{"id":"T22990","span":{"begin":1021,"end":1036},"obj":"JJ"},{"id":"T22991","span":{"begin":1037,"end":1045},"obj":"NN"},{"id":"T22992","span":{"begin":1046,"end":1048},"obj":"IN"},{"id":"T22993","span":{"begin":1049,"end":1060},"obj":"NN"},{"id":"T22994","span":{"begin":1062,"end":1063},"obj":"-LRB-"},{"id":"T22995","span":{"begin":1063,"end":1064},"obj":"NN"},{"id":"T22996","span":{"begin":1064,"end":1065},"obj":"-RRB-"},{"id":"T22997","span":{"begin":1066,"end":1072},"obj":"NN"},{"id":"T22998","span":{"begin":1073,"end":1077},"obj":"VB"},{"id":"T22999","span":{"begin":1078,"end":1087},"obj":"VB"},{"id":"T23000","span":{"begin":1088,"end":1092},"obj":"IN"},{"id":"T23001","span":{"begin":1093,"end":1098},"obj":"JJ"},{"id":"T23002","span":{"begin":1099,"end":1103},"obj":"NN"},{"id":"T23003","span":{"begin":1104,"end":1111},"obj":"VB"},{"id":"T23004","span":{"begin":1112,"end":1119},"obj":"IN"},{"id":"T23005","span":{"begin":1120,"end":1122},"obj":"CC"},{"id":"T23006","span":{"begin":1123,"end":1127},"obj":"IN"},{"id":"T23007","span":{"begin":1128,"end":1133},"obj":"NN"},{"id":"T23008","span":{"begin":1134,"end":1137},"obj":"IN"},{"id":"T23009","span":{"begin":1138,"end":1140},"obj":"CD"},{"id":"T23010","span":{"begin":1141,"end":1143},"obj":"CC"},{"id":"T23011","span":{"begin":1144,"end":1146},"obj":"CD"},{"id":"T23012","span":{"begin":1147,"end":1154},"obj":"NN"},{"id":"T23013","span":{"begin":1156,"end":1161},"obj":"NN"},{"id":"T23014","span":{"begin":1162,"end":1165},"obj":"NN"},{"id":"T23015","span":{"begin":1166,"end":1173},"obj":"NN"},{"id":"T23016","span":{"begin":1174,"end":1182},"obj":"NN"},{"id":"T23017","span":{"begin":1183,"end":1186},"obj":"VB"},{"id":"T23018","span":{"begin":1187,"end":1195},"obj":"VB"},{"id":"T23019","span":{"begin":1196,"end":1198},"obj":"IN"},{"id":"T23020","span":{"begin":1199,"end":1203},"obj":"NNP"},{"id":"T23021","span":{"begin":1204,"end":1209},"obj":"NNP"},{"id":"T23022","span":{"begin":1210,"end":1212},"obj":"VB"},{"id":"T23023","span":{"begin":1213,"end":1214},"obj":"DT"},{"id":"T23024","span":{"begin":1215,"end":1229},"obj":"JJ"},{"id":"T23025","span":{"begin":1230,"end":1234},"obj":"NN"},{"id":"T23026","span":{"begin":1235,"end":1239},"obj":"IN"},{"id":"T23027","span":{"begin":1240,"end":1245},"obj":"CD"},{"id":"T23028","span":{"begin":1246,"end":1257},"obj":"JJ"},{"id":"T23029","span":{"begin":1258,"end":1269},"obj":"NN"},{"id":"T23030","span":{"begin":1271,"end":1273},"obj":"NN"},{"id":"T23031","span":{"begin":1273,"end":1274},"obj":"-COLON-"},{"id":"T23032","span":{"begin":1275,"end":1290},"obj":"JJ"},{"id":"T23033","span":{"begin":1291,"end":1292},"obj":"-LRB-"},{"id":"T23034","span":{"begin":1292,"end":1293},"obj":"NN"},{"id":"T23035","span":{"begin":1293,"end":1294},"obj":"-RRB-"},{"id":"T23036","span":{"begin":1295,"end":1300},"obj":"JJ"},{"id":"T23037","span":{"begin":1301,"end":1305},"obj":"NN"},{"id":"T23038","span":{"begin":1306,"end":1317},"obj":"RB"},{"id":"T23039","span":{"begin":1318,"end":1329},"obj":"VB"},{"id":"T23040","span":{"begin":1330,"end":1334},"obj":"IN"},{"id":"T23041","span":{"begin":1335,"end":1344},"obj":"NN"},{"id":"T23042","span":{"begin":1345,"end":1347},"obj":"CC"},{"id":"T23043","span":{"begin":1348,"end":1359},"obj":"NN"},{"id":"T23044","span":{"begin":1360,"end":1370},"obj":"NN"},{"id":"T23045","span":{"begin":1371,"end":1375},"obj":"VB"},{"id":"T23046","span":{"begin":1376,"end":1383},"obj":"VB"},{"id":"T23047","span":{"begin":1384,"end":1388},"obj":"IN"},{"id":"T23048","span":{"begin":1389,"end":1394},"obj":"NN"},{"id":"T23049","span":{"begin":1395,"end":1397},"obj":"IN"},{"id":"T23050","span":{"begin":1398,"end":1404},"obj":"JJ"},{"id":"T23051","span":{"begin":1405,"end":1411},"obj":"NN"},{"id":"T23052","span":{"begin":1412,"end":1415},"obj":"CC"},{"id":"T23053","span":{"begin":1416,"end":1425},"obj":"VB"},{"id":"T23054","span":{"begin":1426,"end":1429},"obj":"IN"},{"id":"T23055","span":{"begin":1430,"end":1431},"obj":"CD"},{"id":"T23056","span":{"begin":1432,"end":1437},"obj":"NN"},{"id":"T23057","span":{"begin":1438,"end":1444},"obj":"IN"},{"id":"T23058","span":{"begin":1445,"end":1448},"obj":"DT"},{"id":"T23059","span":{"begin":1449,"end":1459},"obj":"NN"},{"id":"T23060","span":{"begin":1460,"end":1462},"obj":"IN"},{"id":"T23061","span":{"begin":1463,"end":1469},"obj":"NN"},{"id":"T23062","span":{"begin":1471,"end":1476},"obj":"NN"},{"id":"T23063","span":{"begin":1477,"end":1485},"obj":"NN"},{"id":"T23064","span":{"begin":1486,"end":1489},"obj":"VB"},{"id":"T23065","span":{"begin":1490,"end":1498},"obj":"VB"},{"id":"T23066","span":{"begin":1499,"end":1501},"obj":"IN"},{"id":"T23067","span":{"begin":1502,"end":1511},"obj":"VB"},{"id":"T23068","span":{"begin":1512,"end":1515},"obj":"DT"},{"id":"T23069","span":{"begin":1516,"end":1522},"obj":"NN"},{"id":"T23070","span":{"begin":1523,"end":1525},"obj":"IN"},{"id":"T23071","span":{"begin":1526,"end":1530},"obj":"NN"},{"id":"T23072","span":{"begin":1531,"end":1539},"obj":"VB"},{"id":"T23073","span":{"begin":1540,"end":1544},"obj":"IN"},{"id":"T23074","span":{"begin":1545,"end":1548},"obj":"DT"},{"id":"T23075","span":{"begin":1549,"end":1555},"obj":"NN"},{"id":"T23076","span":{"begin":1556,"end":1559},"obj":"CC"},{"id":"T23077","span":{"begin":1560,"end":1564},"obj":"NN"},{"id":"T23078","span":{"begin":1565,"end":1568},"obj":"VB"},{"id":"T23079","span":{"begin":1569,"end":1578},"obj":"VB"},{"id":"T23080","span":{"begin":1579,"end":1581},"obj":"IN"},{"id":"T23081","span":{"begin":1582,"end":1586},"obj":"JJ"},{"id":"T23082","span":{"begin":1587,"end":1595},"obj":"NN"},{"id":"T23083","span":{"begin":1596,"end":1598},"obj":"IN"},{"id":"T23084","span":{"begin":1599,"end":1603},"obj":"NN"},{"id":"T23085","span":{"begin":1604,"end":1612},"obj":"NN"},{"id":"T23086","span":{"begin":1613,"end":1617},"obj":"IN"},{"id":"T23087","span":{"begin":1618,"end":1622},"obj":"DT"},{"id":"T23088","span":{"begin":1623,"end":1625},"obj":"IN"},{"id":"T23089","span":{"begin":1626,"end":1635},"obj":"NN"},{"id":"T23090","span":{"begin":1636,"end":1647},"obj":"VB"},{"id":"T23091","span":{"begin":1648,"end":1655},"obj":"VB"},{"id":"T23092","span":{"begin":1656,"end":1662},"obj":"NN"},{"id":"T23093","span":{"begin":1663,"end":1664},"obj":"-LRB-"},{"id":"T23094","span":{"begin":1664,"end":1665},"obj":"NN"},{"id":"T23095","span":{"begin":1665,"end":1666},"obj":"-RRB-"},{"id":"T23096","span":{"begin":1667,"end":1670},"obj":"NN"},{"id":"T23097","span":{"begin":1671,"end":1676},"obj":"CD"},{"id":"T23098","span":{"begin":1677,"end":1682},"obj":"NN"},{"id":"T23099","span":{"begin":1683,"end":1687},"obj":"VB"},{"id":"T23100","span":{"begin":1688,"end":1699},"obj":"RB"},{"id":"T23101","span":{"begin":1700,"end":1711},"obj":"VB"},{"id":"T23102","span":{"begin":1712,"end":1716},"obj":"IN"},{"id":"T23103","span":{"begin":1717,"end":1726},"obj":"NN"},{"id":"T23104","span":{"begin":1727,"end":1729},"obj":"CC"},{"id":"T23105","span":{"begin":1730,"end":1741},"obj":"NN"},{"id":"T23106","span":{"begin":1742,"end":1751},"obj":"NN"},{"id":"T23107","span":{"begin":1753,"end":1758},"obj":"NN"},{"id":"T23108","span":{"begin":1759,"end":1763},"obj":"VB"},{"id":"T23109","span":{"begin":1764,"end":1769},"obj":"NN"},{"id":"T23110","span":{"begin":1770,"end":1777},"obj":"VB"},{"id":"T23111","span":{"begin":1778,"end":1781},"obj":"IN"},{"id":"T23112","span":{"begin":1782,"end":1783},"obj":"CD"},{"id":"T23113","span":{"begin":1784,"end":1789},"obj":"NN"},{"id":"T23114","span":{"begin":1790,"end":1795},"obj":"RB"},{"id":"T23115","span":{"begin":1796,"end":1798},"obj":"TO"},{"id":"T23116","span":{"begin":1799,"end":1800},"obj":"CD"},{"id":"T23117","span":{"begin":1801,"end":1806},"obj":"NN"},{"id":"T23118","span":{"begin":1807,"end":1818},"obj":"NN"},{"id":"T23119","span":{"begin":1819,"end":1823},"obj":"IN"},{"id":"T23120","span":{"begin":1824,"end":1829},"obj":"NN"},{"id":"T23121","span":{"begin":1830,"end":1841},"obj":"JJ"},{"id":"T23122","span":{"begin":1842,"end":1847},"obj":"NN"},{"id":"T23123","span":{"begin":1848,"end":1849},"obj":"-LRB-"},{"id":"T23124","span":{"begin":1849,"end":1852},"obj":"CD"},{"id":"T23125","span":{"begin":1853,"end":1858},"obj":"NN"},{"id":"T23126","span":{"begin":1858,"end":1859},"obj":"-RRB-"},{"id":"T23127","span":{"begin":1861,"end":1868},"obj":"NN"},{"id":"T23128","span":{"begin":1869,"end":1874},"obj":"NN"},{"id":"T23129","span":{"begin":1875,"end":1878},"obj":"VB"},{"id":"T23130","span":{"begin":1879,"end":1888},"obj":"VB"},{"id":"T23131","span":{"begin":1889,"end":1891},"obj":"TO"},{"id":"T23132","span":{"begin":1892,"end":1899},"obj":"VB"},{"id":"T23133","span":{"begin":1900,"end":1904},"obj":"NN"},{"id":"T23134","span":{"begin":1905,"end":1915},"obj":"NN"},{"id":"T23135","span":{"begin":1917,"end":1921},"obj":"NN"},{"id":"T23136","span":{"begin":1922,"end":1925},"obj":"VB"},{"id":"T23137","span":{"begin":1926,"end":1935},"obj":"VB"},{"id":"T23138","span":{"begin":1936,"end":1940},"obj":"JJ"},{"id":"T23139","span":{"begin":1941,"end":1942},"obj":"JJ"},{"id":"T23140","span":{"begin":1943,"end":1948},"obj":"NN"},{"id":"T23141","span":{"begin":1948,"end":1949},"obj":"-COMMA-"},{"id":"T23142","span":{"begin":1950,"end":1953},"obj":"IN"},{"id":"T23143","span":{"begin":1954,"end":1959},"obj":"CD"},{"id":"T23144","span":{"begin":1960,"end":1971},"obj":"JJ"},{"id":"T23145","span":{"begin":1972,"end":1983},"obj":"NN"}],"relations":[{"id":"R3665","pred":"arg1Of","subj":"T4910","obj":"T4911"},{"id":"R3666","pred":"arg2Of","subj":"T4914","obj":"T4911"},{"id":"R3667","pred":"arg1Of","subj":"T4914","obj":"T4912"},{"id":"R3668","pred":"arg1Of","subj":"T4914","obj":"T4913"},{"id":"R3669","pred":"arg1Of","subj":"T4914","obj":"T4915"},{"id":"R3670","pred":"arg1Of","subj":"T4918","obj":"T4916"},{"id":"R3671","pred":"arg1Of","subj":"T4918","obj":"T4917"},{"id":"R3672","pred":"arg1Of","subj":"T4918","obj":"T4919"},{"id":"R3673","pred":"arg1Of","subj":"T4918","obj":"T4922"},{"id":"R3674","pred":"arg2Of","subj":"T4920","obj":"T4919"},{"id":"R3675","pred":"arg3Of","subj":"T4921","obj":"T4919"},{"id":"R3676","pred":"arg2Of","subj":"T4922","obj":"T4915"},{"id":"R3677","pred":"arg2Of","subj":"T4926","obj":"T4922"},{"id":"R3678","pred":"arg1Of","subj":"T4926","obj":"T4923"},{"id":"R3679","pred":"arg1Of","subj":"T4926","obj":"T4924"},{"id":"R3680","pred":"arg1Of","subj":"T4926","obj":"T4925"},{"id":"R3681","pred":"arg1Of","subj":"T4926","obj":"T4927"},{"id":"R3682","pred":"arg2Of","subj":"T4928","obj":"T4927"},{"id":"R3683","pred":"arg1Of","subj":"T4928","obj":"T4929"},{"id":"R3684","pred":"arg1Of","subj":"T4931","obj":"T4930"},{"id":"R3685","pred":"arg1Of","subj":"T4933","obj":"T4934"},{"id":"R3686","pred":"arg2Of","subj":"T4934","obj":"T4931"},{"id":"R3687","pred":"arg1Of","subj":"T4934","obj":"T4932"},{"id":"R3688","pred":"arg1Of","subj":"T4934","obj":"T4938"},{"id":"R3689","pred":"arg2Of","subj":"T4937","obj":"T4934"},{"id":"R3690","pred":"arg1Of","subj":"T4937","obj":"T4935"},{"id":"R3691","pred":"arg1Of","subj":"T4937","obj":"T4936"},{"id":"R3692","pred":"arg2Of","subj":"T4940","obj":"T4938"},{"id":"R3693","pred":"arg1Of","subj":"T4940","obj":"T4939"},{"id":"R3694","pred":"arg1Of","subj":"T4942","obj":"T4931"},{"id":"R3695","pred":"arg1Of","subj":"T4942","obj":"T4943"},{"id":"R3696","pred":"modOf","subj":"T4943","obj":"T4930"},{"id":"R3697","pred":"arg1Of","subj":"T4943","obj":"T4941"},{"id":"R3698","pred":"arg2Of","subj":"T4945","obj":"T4943"},{"id":"R3699","pred":"arg1Of","subj":"T4945","obj":"T4944"},{"id":"R3700","pred":"arg1Of","subj":"T4945","obj":"T4946"},{"id":"R3701","pred":"arg2Of","subj":"T4948","obj":"T4946"},{"id":"R3702","pred":"arg1Of","subj":"T4948","obj":"T4947"},{"id":"R3703","pred":"arg1Of","subj":"T4948","obj":"T4949"},{"id":"R3704","pred":"arg1Of","subj":"T4948","obj":"T4952"},{"id":"R3705","pred":"arg1Of","subj":"T4948","obj":"T4959"},{"id":"R3706","pred":"arg2Of","subj":"T4951","obj":"T4949"},{"id":"R3707","pred":"arg1Of","subj":"T4951","obj":"T4950"},{"id":"R3708","pred":"arg1Of","subj":"T4952","obj":"T4953"},{"id":"R3709","pred":"arg2Of","subj":"T4955","obj":"T4953"},{"id":"R3710","pred":"arg1Of","subj":"T4955","obj":"T4954"},{"id":"R3711","pred":"arg1Of","subj":"T4955","obj":"T4956"},{"id":"R3712","pred":"arg2Of","subj":"T4957","obj":"T4956"},{"id":"R3713","pred":"arg3Of","subj":"T4958","obj":"T4956"},{"id":"R3714","pred":"arg1Of","subj":"T4961","obj":"T4960"},{"id":"R3715","pred":"arg1Of","subj":"T4961","obj":"T4963"},{"id":"R3716","pred":"arg2Of","subj":"T4963","obj":"T4959"},{"id":"R3717","pred":"arg1Of","subj":"T4963","obj":"T4962"},{"id":"R3718","pred":"arg1Of","subj":"T4963","obj":"T4965"},{"id":"R3719","pred":"arg2Of","subj":"T4964","obj":"T4963"},{"id":"R3720","pred":"arg2Of","subj":"T4967","obj":"T4965"},{"id":"R3721","pred":"arg1Of","subj":"T4967","obj":"T4966"},{"id":"R3722","pred":"arg1Of","subj":"T4967","obj":"T4968"},{"id":"R3723","pred":"arg2Of","subj":"T4970","obj":"T4968"},{"id":"R3724","pred":"arg1Of","subj":"T4970","obj":"T4969"},{"id":"R3725","pred":"arg3Of","subj":"T4971","obj":"T4968"},{"id":"R3726","pred":"arg1Of","subj":"T4975","obj":"T4974"},{"id":"R3727","pred":"arg1Of","subj":"T4975","obj":"T4976"},{"id":"R3728","pred":"arg1Of","subj":"T4975","obj":"T4978"},{"id":"R3729","pred":"arg1Of","subj":"T4978","obj":"T4972"},{"id":"R3730","pred":"arg1Of","subj":"T4978","obj":"T4973"},{"id":"R3731","pred":"arg2Of","subj":"T4978","obj":"T4976"},{"id":"R3732","pred":"arg1Of","subj":"T4978","obj":"T4977"},{"id":"R3733","pred":"arg1Of","subj":"T4978","obj":"T4982"},{"id":"R3734","pred":"arg2Of","subj":"T4981","obj":"T4978"},{"id":"R3735","pred":"arg1Of","subj":"T4981","obj":"T4979"},{"id":"R3736","pred":"arg1Of","subj":"T4981","obj":"T4980"},{"id":"R3737","pred":"arg2Of","subj":"T4983","obj":"T4982"},{"id":"R3738","pred":"arg2Of","subj":"T4985","obj":"T4984"},{"id":"R3739","pred":"arg2Of","subj":"T4989","obj":"T4987"},{"id":"R3740","pred":"arg1Of","subj":"T4989","obj":"T4988"},{"id":"R3741","pred":"arg1Of","subj":"T4989","obj":"T4992"},{"id":"R3742","pred":"arg2Of","subj":"T4991","obj":"T4989"},{"id":"R3743","pred":"arg1Of","subj":"T4991","obj":"T4990"},{"id":"R3744","pred":"arg2Of","subj":"T4994","obj":"T4992"},{"id":"R3745","pred":"arg1Of","subj":"T4994","obj":"T4993"},{"id":"R3746","pred":"arg1Of","subj":"T4994","obj":"T4995"},{"id":"R3747","pred":"arg2Of","subj":"T5000","obj":"T4995"},{"id":"R3748","pred":"arg1Of","subj":"T5000","obj":"T4996"},{"id":"R3749","pred":"arg1Of","subj":"T5000","obj":"T4997"},{"id":"R3750","pred":"arg1Of","subj":"T5000","obj":"T4998"},{"id":"R3751","pred":"arg1Of","subj":"T5000","obj":"T4999"},{"id":"R3752","pred":"arg1Of","subj":"T5003","obj":"T4989"},{"id":"R3753","pred":"arg1Of","subj":"T5003","obj":"T5002"},{"id":"R3754","pred":"arg1Of","subj":"T5003","obj":"T5004"},{"id":"R3755","pred":"arg1Of","subj":"T5003","obj":"T5008"},{"id":"R3756","pred":"arg2Of","subj":"T5007","obj":"T5004"},{"id":"R3757","pred":"arg1Of","subj":"T5007","obj":"T5005"},{"id":"R3758","pred":"arg2Of","subj":"T5007","obj":"T5006"},{"id":"R3759","pred":"arg1Of","subj":"T5008","obj":"T4984"},{"id":"R3760","pred":"arg1Of","subj":"T5008","obj":"T4986"},{"id":"R3761","pred":"arg1Of","subj":"T5008","obj":"T4987"},{"id":"R3762","pred":"arg1Of","subj":"T5008","obj":"T5001"},{"id":"R3763","pred":"arg1Of","subj":"T5008","obj":"T5009"},{"id":"R3764","pred":"arg1Of","subj":"T5008","obj":"T5020"},{"id":"R3765","pred":"arg2Of","subj":"T5012","obj":"T5009"},{"id":"R3766","pred":"arg1Of","subj":"T5012","obj":"T5010"},{"id":"R3767","pred":"arg1Of","subj":"T5012","obj":"T5011"},{"id":"R3768","pred":"arg1Of","subj":"T5012","obj":"T5013"},{"id":"R3769","pred":"arg1Of","subj":"T5012","obj":"T5016"},{"id":"R3770","pred":"arg2Of","subj":"T5015","obj":"T5013"},{"id":"R3771","pred":"arg1Of","subj":"T5015","obj":"T5014"},{"id":"R3772","pred":"arg2Of","subj":"T5019","obj":"T5016"},{"id":"R3773","pred":"arg1Of","subj":"T5019","obj":"T5017"},{"id":"R3774","pred":"arg1Of","subj":"T5019","obj":"T5018"},{"id":"R3775","pred":"arg2Of","subj":"T5021","obj":"T5020"},{"id":"R3776","pred":"arg2Of","subj":"T5024","obj":"T5023"},{"id":"R3777","pred":"arg3Of","subj":"T5025","obj":"T5023"},{"id":"R3778","pred":"arg2Of","subj":"T5028","obj":"T5021"},{"id":"R3779","pred":"arg1Of","subj":"T5028","obj":"T5022"},{"id":"R3780","pred":"arg1Of","subj":"T5028","obj":"T5023"},{"id":"R3781","pred":"arg1Of","subj":"T5028","obj":"T5026"},{"id":"R3782","pred":"arg2Of","subj":"T5028","obj":"T5027"},{"id":"R3783","pred":"arg1Of","subj":"T5028","obj":"T5029"},{"id":"R3784","pred":"arg2Of","subj":"T5031","obj":"T5029"},{"id":"R3785","pred":"arg1Of","subj":"T5031","obj":"T5030"},{"id":"R3786","pred":"arg3Of","subj":"T5032","obj":"T5029"},{"id":"R3787","pred":"arg2Of","subj":"T5035","obj":"T5033"},{"id":"R3788","pred":"arg1Of","subj":"T5035","obj":"T5034"},{"id":"R3789","pred":"arg1Of","subj":"T5039","obj":"T5037"},{"id":"R3790","pred":"arg1Of","subj":"T5039","obj":"T5038"},{"id":"R3791","pred":"arg1Of","subj":"T5039","obj":"T5040"},{"id":"R3792","pred":"arg1Of","subj":"T5039","obj":"T5044"},{"id":"R3793","pred":"arg2Of","subj":"T5039","obj":"T5045"},{"id":"R3794","pred":"arg2Of","subj":"T5043","obj":"T5040"},{"id":"R3795","pred":"arg1Of","subj":"T5043","obj":"T5041"},{"id":"R3796","pred":"arg1Of","subj":"T5043","obj":"T5042"},{"id":"R3797","pred":"arg1Of","subj":"T5045","obj":"T5033"},{"id":"R3798","pred":"arg1Of","subj":"T5045","obj":"T5036"},{"id":"R3799","pred":"arg2Of","subj":"T5045","obj":"T5044"},{"id":"R3800","pred":"arg2Of","subj":"T5046","obj":"T5047"},{"id":"R3801","pred":"arg1Of","subj":"T5046","obj":"T5052"},{"id":"R3802","pred":"arg1Of","subj":"T5046","obj":"T5054"},{"id":"R3803","pred":"arg1Of","subj":"T5047","obj":"T5048"},{"id":"R3804","pred":"arg2Of","subj":"T5051","obj":"T5048"},{"id":"R3805","pred":"arg1Of","subj":"T5051","obj":"T5049"},{"id":"R3806","pred":"arg1Of","subj":"T5051","obj":"T5050"},{"id":"R3807","pred":"arg2Of","subj":"T5054","obj":"T5052"},{"id":"R3808","pred":"arg1Of","subj":"T5054","obj":"T5053"},{"id":"R3809","pred":"arg1Of","subj":"T5054","obj":"T5056"},{"id":"R3810","pred":"arg2Of","subj":"T5055","obj":"T5054"},{"id":"R3811","pred":"arg1Of","subj":"T5058","obj":"T5059"},{"id":"R3812","pred":"arg2Of","subj":"T5059","obj":"T5056"},{"id":"R3813","pred":"arg1Of","subj":"T5059","obj":"T5057"},{"id":"R3814","pred":"arg1Of","subj":"T5059","obj":"T5061"},{"id":"R3815","pred":"arg2Of","subj":"T5060","obj":"T5059"},{"id":"R3816","pred":"arg2Of","subj":"T5062","obj":"T5061"},{"id":"R3817","pred":"arg1Of","subj":"T5062","obj":"T5063"},{"id":"R3818","pred":"arg2Of","subj":"T5065","obj":"T5063"},{"id":"R3819","pred":"arg1Of","subj":"T5065","obj":"T5064"},{"id":"R3820","pred":"arg3Of","subj":"T5066","obj":"T5063"},{"id":"R3821","pred":"arg1Of","subj":"T5067","obj":"T5069"},{"id":"R3822","pred":"arg1Of","subj":"T5069","obj":"T5068"},{"id":"R3823","pred":"arg1Of","subj":"T5071","obj":"T5072"},{"id":"R3824","pred":"arg2Of","subj":"T5072","obj":"T5069"},{"id":"R3825","pred":"arg1Of","subj":"T5072","obj":"T5070"},{"id":"R3826","pred":"arg1Of","subj":"T5072","obj":"T5075"},{"id":"R3827","pred":"arg2Of","subj":"T5074","obj":"T5072"},{"id":"R3828","pred":"arg1Of","subj":"T5074","obj":"T5073"},{"id":"R3829","pred":"arg2Of","subj":"T5080","obj":"T5075"},{"id":"R3830","pred":"arg1Of","subj":"T5080","obj":"T5076"},{"id":"R3831","pred":"arg1Of","subj":"T5080","obj":"T5077"},{"id":"R3832","pred":"arg1Of","subj":"T5080","obj":"T5078"},{"id":"R3833","pred":"arg1Of","subj":"T5080","obj":"T5079"},{"id":"R3834","pred":"arg1Of","subj":"T5080","obj":"T5081"},{"id":"R3835","pred":"arg2Of","subj":"T5082","obj":"T5081"},{"id":"R3836","pred":"arg1Of","subj":"T5082","obj":"T5083"},{"id":"R3837","pred":"arg1Of","subj":"T5086","obj":"T5084"},{"id":"R3838","pred":"arg1Of","subj":"T5086","obj":"T5085"},{"id":"R3839","pred":"arg1Of","subj":"T5086","obj":"T5087"},{"id":"R3840","pred":"arg2Of","subj":"T5086","obj":"T5088"},{"id":"R3841","pred":"arg2Of","subj":"T5088","obj":"T5087"},{"id":"R3842","pred":"arg1Of","subj":"T5088","obj":"T5089"},{"id":"R3843","pred":"arg1Of","subj":"T5093","obj":"T5092"},{"id":"R3844","pred":"arg1Of","subj":"T5093","obj":"T5094"},{"id":"R3845","pred":"arg2Of","subj":"T5094","obj":"T5089"},{"id":"R3846","pred":"arg1Of","subj":"T5094","obj":"T5090"},{"id":"R3847","pred":"arg1Of","subj":"T5094","obj":"T5091"},{"id":"R3848","pred":"arg2Of","subj":"T5097","obj":"T5094"},{"id":"R3849","pred":"arg1Of","subj":"T5097","obj":"T5095"},{"id":"R3850","pred":"arg1Of","subj":"T5097","obj":"T5096"},{"id":"R3851","pred":"arg2Of","subj":"T5099","obj":"T5098"},{"id":"R3852","pred":"arg1Of","subj":"T5099","obj":"T5100"},{"id":"R3853","pred":"arg2Of","subj":"T5102","obj":"T5100"},{"id":"R3854","pred":"arg1Of","subj":"T5102","obj":"T5101"},{"id":"R3855","pred":"arg1Of","subj":"T5105","obj":"T5104"},{"id":"R3856","pred":"arg1Of","subj":"T5105","obj":"T5106"},{"id":"R3857","pred":"arg1Of","subj":"T5105","obj":"T5110"},{"id":"R3858","pred":"arg2Of","subj":"T5109","obj":"T5106"},{"id":"R3859","pred":"arg1Of","subj":"T5109","obj":"T5107"},{"id":"R3860","pred":"arg1Of","subj":"T5109","obj":"T5108"},{"id":"R3861","pred":"arg1Of","subj":"T5110","obj":"T5098"},{"id":"R3862","pred":"arg1Of","subj":"T5110","obj":"T5103"},{"id":"R3863","pred":"arg1Of","subj":"T5110","obj":"T5114"},{"id":"R3864","pred":"arg1Of","subj":"T5110","obj":"T5116"},{"id":"R3865","pred":"arg2Of","subj":"T5113","obj":"T5110"},{"id":"R3866","pred":"arg1Of","subj":"T5113","obj":"T5111"},{"id":"R3867","pred":"arg1Of","subj":"T5113","obj":"T5112"},{"id":"R3868","pred":"arg2Of","subj":"T5115","obj":"T5114"},{"id":"R3869","pred":"arg2Of","subj":"T5117","obj":"T5116"},{"id":"R3870","pred":"arg1Of","subj":"T5117","obj":"T5118"},{"id":"R3871","pred":"arg2Of","subj":"T5120","obj":"T5118"},{"id":"R3872","pred":"arg1Of","subj":"T5120","obj":"T5119"},{"id":"R3873","pred":"arg1Of","subj":"T5124","obj":"T5123"},{"id":"R3874","pred":"arg1Of","subj":"T5124","obj":"T5125"},{"id":"R3875","pred":"arg1Of","subj":"T5125","obj":"T5121"},{"id":"R3876","pred":"arg1Of","subj":"T5125","obj":"T5122"},{"id":"R3877","pred":"arg1Of","subj":"T5127","obj":"T5128"},{"id":"R3878","pred":"arg2Of","subj":"T5128","obj":"T5125"},{"id":"R3879","pred":"arg1Of","subj":"T5128","obj":"T5126"},{"id":"R3880","pred":"arg2Of","subj":"T5131","obj":"T5128"},{"id":"R3881","pred":"arg1Of","subj":"T5131","obj":"T5129"},{"id":"R3882","pred":"arg1Of","subj":"T5131","obj":"T5130"},{"id":"R3883","pred":"arg1Of","subj":"T5131","obj":"T5132"},{"id":"R3884","pred":"arg2Of","subj":"T5133","obj":"T5132"},{"id":"R17531","pred":"arg1Of","subj":"T22987","obj":"T22988"},{"id":"R17532","pred":"arg1Of","subj":"T22988","obj":"T22992"},{"id":"R17533","pred":"arg2Of","subj":"T22991","obj":"T22988"},{"id":"R17534","pred":"arg1Of","subj":"T22991","obj":"T22989"},{"id":"R17535","pred":"arg1Of","subj":"T22991","obj":"T22990"},{"id":"R17536","pred":"arg2Of","subj":"T22993","obj":"T22992"},{"id":"R17537","pred":"arg2Of","subj":"T22995","obj":"T22994"},{"id":"R17538","pred":"arg3Of","subj":"T22996","obj":"T22994"},{"id":"R17539","pred":"arg1Of","subj":"T22997","obj":"T22994"},{"id":"R17540","pred":"arg1Of","subj":"T22997","obj":"T22998"},{"id":"R17541","pred":"arg2Of","subj":"T22997","obj":"T22999"},{"id":"R17542","pred":"arg2Of","subj":"T22999","obj":"T22998"},{"id":"R17543","pred":"arg1Of","subj":"T22999","obj":"T23000"},{"id":"R17544","pred":"arg2Of","subj":"T23002","obj":"T23000"},{"id":"R17545","pred":"arg1Of","subj":"T23002","obj":"T23001"},{"id":"R17546","pred":"arg2Of","subj":"T23002","obj":"T23003"},{"id":"R17547","pred":"arg1Of","subj":"T23003","obj":"T23004"},{"id":"R17548","pred":"arg1Of","subj":"T23003","obj":"T23006"},{"id":"R17549","pred":"arg1Of","subj":"T23004","obj":"T23005"},{"id":"R17550","pred":"arg2Of","subj":"T23006","obj":"T23005"},{"id":"R17551","pred":"arg2Of","subj":"T23007","obj":"T23004"},{"id":"R17552","pred":"arg2Of","subj":"T23007","obj":"T23006"},{"id":"R17553","pred":"arg1Of","subj":"T23007","obj":"T23008"},{"id":"R17554","pred":"arg1Of","subj":"T23009","obj":"T23010"},{"id":"R17555","pred":"arg2Of","subj":"T23011","obj":"T23010"},{"id":"R17556","pred":"arg2Of","subj":"T23012","obj":"T23008"},{"id":"R17557","pred":"arg1Of","subj":"T23012","obj":"T23009"},{"id":"R17558","pred":"arg1Of","subj":"T23012","obj":"T23011"},{"id":"R17559","pred":"arg1Of","subj":"T23013","obj":"T23022"},{"id":"R17560","pred":"arg1Of","subj":"T23016","obj":"T23014"},{"id":"R17561","pred":"arg1Of","subj":"T23016","obj":"T23015"},{"id":"R17562","pred":"arg1Of","subj":"T23016","obj":"T23017"},{"id":"R17563","pred":"arg2Of","subj":"T23016","obj":"T23018"},{"id":"R17564","pred":"arg2Of","subj":"T23018","obj":"T23017"},{"id":"R17565","pred":"arg1Of","subj":"T23021","obj":"T23018"},{"id":"R17566","pred":"arg2Of","subj":"T23021","obj":"T23019"},{"id":"R17567","pred":"arg1Of","subj":"T23021","obj":"T23020"},{"id":"R17568","pred":"modOf","subj":"T23022","obj":"T23017"},{"id":"R17569","pred":"arg2Of","subj":"T23025","obj":"T23022"},{"id":"R17570","pred":"arg1Of","subj":"T23025","obj":"T23023"},{"id":"R17571","pred":"arg1Of","subj":"T23025","obj":"T23024"},{"id":"R17572","pred":"arg1Of","subj":"T23025","obj":"T23026"},{"id":"R17573","pred":"arg2Of","subj":"T23029","obj":"T23026"},{"id":"R17574","pred":"arg1Of","subj":"T23029","obj":"T23027"},{"id":"R17575","pred":"arg1Of","subj":"T23029","obj":"T23028"},{"id":"R17576","pred":"arg1Of","subj":"T23030","obj":"T23031"},{"id":"R17577","pred":"modOf","subj":"T23030","obj":"T23045"},{"id":"R17578","pred":"arg2Of","subj":"T23034","obj":"T23033"},{"id":"R17579","pred":"arg3Of","subj":"T23035","obj":"T23033"},{"id":"R17580","pred":"arg1Of","subj":"T23037","obj":"T23032"},{"id":"R17581","pred":"arg1Of","subj":"T23037","obj":"T23033"},{"id":"R17582","pred":"arg1Of","subj":"T23037","obj":"T23036"},{"id":"R17583","pred":"arg2Of","subj":"T23037","obj":"T23039"},{"id":"R17584","pred":"arg1Of","subj":"T23037","obj":"T23045"},{"id":"R17585","pred":"arg2Of","subj":"T23037","obj":"T23046"},{"id":"R17586","pred":"arg2Of","subj":"T23037","obj":"T23053"},{"id":"R17587","pred":"arg1Of","subj":"T23039","obj":"T23038"},{"id":"R17588","pred":"arg1Of","subj":"T23039","obj":"T23040"},{"id":"R17589","pred":"arg1Of","subj":"T23041","obj":"T23042"},{"id":"R17590","pred":"arg2Of","subj":"T23043","obj":"T23042"},{"id":"R17591","pred":"arg2Of","subj":"T23044","obj":"T23040"},{"id":"R17592","pred":"arg1Of","subj":"T23044","obj":"T23041"},{"id":"R17593","pred":"arg1Of","subj":"T23044","obj":"T23043"},{"id":"R17594","pred":"arg1Of","subj":"T23046","obj":"T23047"},{"id":"R17595","pred":"arg1Of","subj":"T23046","obj":"T23052"},{"id":"R17596","pred":"arg2Of","subj":"T23048","obj":"T23047"},{"id":"R17597","pred":"arg1Of","subj":"T23048","obj":"T23049"},{"id":"R17598","pred":"arg2Of","subj":"T23051","obj":"T23049"},{"id":"R17599","pred":"arg1Of","subj":"T23051","obj":"T23050"},{"id":"R17600","pred":"arg2Of","subj":"T23052","obj":"T23045"},{"id":"R17601","pred":"arg2Of","subj":"T23053","obj":"T23052"},{"id":"R17602","pred":"arg1Of","subj":"T23053","obj":"T23054"},{"id":"R17603","pred":"arg1Of","subj":"T23053","obj":"T23057"},{"id":"R17604","pred":"arg2Of","subj":"T23056","obj":"T23054"},{"id":"R17605","pred":"arg1Of","subj":"T23056","obj":"T23055"},{"id":"R17606","pred":"arg2Of","subj":"T23059","obj":"T23057"},{"id":"R17607","pred":"arg1Of","subj":"T23059","obj":"T23058"},{"id":"R17608","pred":"arg1Of","subj":"T23059","obj":"T23060"},{"id":"R17609","pred":"arg2Of","subj":"T23061","obj":"T23060"},{"id":"R17610","pred":"arg1Of","subj":"T23063","obj":"T23062"},{"id":"R17611","pred":"arg1Of","subj":"T23063","obj":"T23064"},{"id":"R17612","pred":"arg2Of","subj":"T23063","obj":"T23065"},{"id":"R17613","pred":"arg2Of","subj":"T23065","obj":"T23064"},{"id":"R17614","pred":"arg1Of","subj":"T23065","obj":"T23066"},{"id":"R17615","pred":"arg1Of","subj":"T23065","obj":"T23076"},{"id":"R17616","pred":"arg2Of","subj":"T23067","obj":"T23066"},{"id":"R17617","pred":"arg2Of","subj":"T23069","obj":"T23067"},{"id":"R17618","pred":"arg1Of","subj":"T23069","obj":"T23068"},{"id":"R17619","pred":"arg1Of","subj":"T23069","obj":"T23070"},{"id":"R17620","pred":"arg2Of","subj":"T23069","obj":"T23072"},{"id":"R17621","pred":"arg2Of","subj":"T23071","obj":"T23070"},{"id":"R17622","pred":"arg1Of","subj":"T23072","obj":"T23073"},{"id":"R17623","pred":"arg2Of","subj":"T23075","obj":"T23073"},{"id":"R17624","pred":"arg1Of","subj":"T23075","obj":"T23074"},{"id":"R17625","pred":"arg1Of","subj":"T23077","obj":"T23078"},{"id":"R17626","pred":"arg2Of","subj":"T23077","obj":"T23079"},{"id":"R17627","pred":"arg2Of","subj":"T23079","obj":"T23076"},{"id":"R17628","pred":"arg2Of","subj":"T23079","obj":"T23078"},{"id":"R17629","pred":"arg1Of","subj":"T23079","obj":"T23080"},{"id":"R17630","pred":"arg1Of","subj":"T23082","obj":"T23083"},{"id":"R17631","pred":"arg1Of","subj":"T23082","obj":"T23086"},{"id":"R17632","pred":"arg2Of","subj":"T23085","obj":"T23083"},{"id":"R17633","pred":"arg1Of","subj":"T23085","obj":"T23084"},{"id":"R17634","pred":"arg2Of","subj":"T23087","obj":"T23086"},{"id":"R17635","pred":"arg1Of","subj":"T23087","obj":"T23088"},{"id":"R17636","pred":"arg2Of","subj":"T23092","obj":"T23088"},{"id":"R17637","pred":"arg1Of","subj":"T23092","obj":"T23089"},{"id":"R17638","pred":"arg2Of","subj":"T23092","obj":"T23090"},{"id":"R17639","pred":"arg1Of","subj":"T23092","obj":"T23091"},{"id":"R17640","pred":"arg1Of","subj":"T23092","obj":"T23093"},{"id":"R17641","pred":"arg2Of","subj":"T23094","obj":"T23093"},{"id":"R17642","pred":"arg3Of","subj":"T23095","obj":"T23093"},{"id":"R17643","pred":"arg1Of","subj":"T23098","obj":"T23081"},{"id":"R17644","pred":"arg1Of","subj":"T23098","obj":"T23082"},{"id":"R17645","pred":"arg1Of","subj":"T23098","obj":"T23096"},{"id":"R17646","pred":"arg1Of","subj":"T23098","obj":"T23097"},{"id":"R17647","pred":"arg1Of","subj":"T23098","obj":"T23099"},{"id":"R17648","pred":"arg2Of","subj":"T23098","obj":"T23101"},{"id":"R17649","pred":"arg2Of","subj":"T23101","obj":"T23080"},{"id":"R17650","pred":"arg2Of","subj":"T23101","obj":"T23099"},{"id":"R17651","pred":"arg1Of","subj":"T23101","obj":"T23100"},{"id":"R17652","pred":"arg1Of","subj":"T23101","obj":"T23102"},{"id":"R17653","pred":"arg1Of","subj":"T23103","obj":"T23104"},{"id":"R17654","pred":"arg2Of","subj":"T23104","obj":"T23102"},{"id":"R17655","pred":"arg2Of","subj":"T23106","obj":"T23104"},{"id":"R17656","pred":"arg1Of","subj":"T23106","obj":"T23105"},{"id":"R17657","pred":"arg1Of","subj":"T23107","obj":"T23108"},{"id":"R17658","pred":"arg2Of","subj":"T23109","obj":"T23108"},{"id":"R17659","pred":"arg2Of","subj":"T23109","obj":"T23110"},{"id":"R17660","pred":"arg1Of","subj":"T23110","obj":"T23111"},{"id":"R17661","pred":"arg1Of","subj":"T23112","obj":"T23115"},{"id":"R17662","pred":"arg1Of","subj":"T23114","obj":"T23113"},{"id":"R17663","pred":"arg1Of","subj":"T23115","obj":"T23114"},{"id":"R17664","pred":"arg2Of","subj":"T23117","obj":"T23115"},{"id":"R17665","pred":"arg1Of","subj":"T23117","obj":"T23116"},{"id":"R17666","pred":"arg2Of","subj":"T23118","obj":"T23111"},{"id":"R17667","pred":"arg1Of","subj":"T23118","obj":"T23112"},{"id":"R17668","pred":"arg1Of","subj":"T23118","obj":"T23119"},{"id":"R17669","pred":"arg2Of","subj":"T23122","obj":"T23119"},{"id":"R17670","pred":"arg1Of","subj":"T23122","obj":"T23120"},{"id":"R17671","pred":"arg1Of","subj":"T23122","obj":"T23121"},{"id":"R17672","pred":"arg1Of","subj":"T23122","obj":"T23123"},{"id":"R17673","pred":"arg2Of","subj":"T23125","obj":"T23123"},{"id":"R17674","pred":"arg1Of","subj":"T23125","obj":"T23124"},{"id":"R17675","pred":"arg3Of","subj":"T23126","obj":"T23123"},{"id":"R17676","pred":"arg1Of","subj":"T23128","obj":"T23127"},{"id":"R17677","pred":"arg1Of","subj":"T23128","obj":"T23129"},{"id":"R17678","pred":"arg2Of","subj":"T23128","obj":"T23130"},{"id":"R17679","pred":"arg2Of","subj":"T23130","obj":"T23129"},{"id":"R17680","pred":"modOf","subj":"T23130","obj":"T23131"},{"id":"R17681","pred":"arg1Of","subj":"T23132","obj":"T23131"},{"id":"R17682","pred":"arg2Of","subj":"T23134","obj":"T23132"},{"id":"R17683","pred":"arg1Of","subj":"T23134","obj":"T23133"},{"id":"R17684","pred":"arg1Of","subj":"T23135","obj":"T23136"},{"id":"R17685","pred":"arg2Of","subj":"T23135","obj":"T23137"},{"id":"R17686","pred":"arg2Of","subj":"T23137","obj":"T23136"},{"id":"R17687","pred":"arg1Of","subj":"T23137","obj":"T23140"},{"id":"R17688","pred":"arg1Of","subj":"T23137","obj":"T23141"},{"id":"R17689","pred":"arg1Of","subj":"T23137","obj":"T23142"},{"id":"R17690","pred":"arg1Of","subj":"T23140","obj":"T23138"},{"id":"R17691","pred":"arg1Of","subj":"T23140","obj":"T23139"},{"id":"R17692","pred":"arg2Of","subj":"T23145","obj":"T23142"},{"id":"R17693","pred":"arg1Of","subj":"T23145","obj":"T23143"},{"id":"R17694","pred":"arg1Of","subj":"T23145","obj":"T23144"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"sentences","denotations":[{"id":"T4664","span":{"begin":0,"end":131},"obj":"Sentence"},{"id":"T4665","span":{"begin":132,"end":386},"obj":"Sentence"},{"id":"T4666","span":{"begin":387,"end":455},"obj":"Sentence"},{"id":"T4667","span":{"begin":456,"end":763},"obj":"Sentence"},{"id":"T4668","span":{"begin":764,"end":839},"obj":"Sentence"},{"id":"T4669","span":{"begin":840,"end":956},"obj":"Sentence"},{"id":"T4670","span":{"begin":1987,"end":2090},"obj":"Sentence"},{"id":"T4671","span":{"begin":2091,"end":2191},"obj":"Sentence"},{"id":"T4672","span":{"begin":2192,"end":2327},"obj":"Sentence"},{"id":"T4673","span":{"begin":2328,"end":2424},"obj":"Sentence"},{"id":"T22810","span":{"begin":1001,"end":1061},"obj":"Sentence"},{"id":"T22811","span":{"begin":1062,"end":1155},"obj":"Sentence"},{"id":"T22812","span":{"begin":1156,"end":1270},"obj":"Sentence"},{"id":"T22813","span":{"begin":1271,"end":1470},"obj":"Sentence"},{"id":"T22814","span":{"begin":1471,"end":1752},"obj":"Sentence"},{"id":"T22815","span":{"begin":1753,"end":1860},"obj":"Sentence"},{"id":"T22816","span":{"begin":1861,"end":1916},"obj":"Sentence"},{"id":"T22817","span":{"begin":1917,"end":1984},"obj":"Sentence"},{"id":"T62","span":{"begin":0,"end":131},"obj":"Sentence"},{"id":"T63","span":{"begin":132,"end":386},"obj":"Sentence"},{"id":"T64","span":{"begin":387,"end":455},"obj":"Sentence"},{"id":"T65","span":{"begin":456,"end":763},"obj":"Sentence"},{"id":"T66","span":{"begin":764,"end":839},"obj":"Sentence"},{"id":"T67","span":{"begin":840,"end":956},"obj":"Sentence"},{"id":"T68","span":{"begin":957,"end":1061},"obj":"Sentence"},{"id":"T69","span":{"begin":1062,"end":1155},"obj":"Sentence"},{"id":"T70","span":{"begin":1156,"end":1270},"obj":"Sentence"},{"id":"T71","span":{"begin":1271,"end":1470},"obj":"Sentence"},{"id":"T72","span":{"begin":1471,"end":1752},"obj":"Sentence"},{"id":"T73","span":{"begin":1753,"end":1860},"obj":"Sentence"},{"id":"T74","span":{"begin":1861,"end":1916},"obj":"Sentence"},{"id":"T75","span":{"begin":1917,"end":1984},"obj":"Sentence"},{"id":"T76","span":{"begin":1987,"end":2090},"obj":"Sentence"},{"id":"T77","span":{"begin":2091,"end":2191},"obj":"Sentence"},{"id":"T78","span":{"begin":2192,"end":2327},"obj":"Sentence"},{"id":"T79","span":{"begin":2328,"end":2424},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"2_test","denotations":[{"id":"22216091-11073986-90609709","span":{"begin":302,"end":304},"obj":"11073986"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T4674","span":{"begin":0,"end":5},"obj":"NNP"},{"id":"T4675","span":{"begin":6,"end":13},"obj":"VBZ"},{"id":"T4676","span":{"begin":14,"end":19},"obj":"NNP"},{"id":"T4677","span":{"begin":20,"end":35},"obj":"NNP"},{"id":"T4678","span":{"begin":36,"end":44},"obj":"NNP"},{"id":"T4679","span":{"begin":45,"end":47},"obj":"IN"},{"id":"T4680","span":{"begin":48,"end":53},"obj":"NNP"},{"id":"T4681","span":{"begin":54,"end":62},"obj":"NNP"},{"id":"T4682","span":{"begin":63,"end":70},"obj":"NNP"},{"id":"T4683","span":{"begin":71,"end":82},"obj":"NNP"},{"id":"T4684","span":{"begin":83,"end":84},"obj":"-LRB-"},{"id":"T4685","span":{"begin":84,"end":88},"obj":"NNP"},{"id":"T4686","span":{"begin":88,"end":89},"obj":"-RRB-"},{"id":"T4687","span":{"begin":90,"end":93},"obj":"CC"},{"id":"T4688","span":{"begin":94,"end":99},"obj":"NNP"},{"id":"T4689","span":{"begin":100,"end":110},"obj":"NNP"},{"id":"T4690","span":{"begin":111,"end":115},"obj":"NNP"},{"id":"T4691","span":{"begin":116,"end":120},"obj":"NNP"},{"id":"T4692","span":{"begin":120,"end":121},"obj":","},{"id":"T4693","span":{"begin":122,"end":125},"obj":"NNP"},{"id":"T4694","span":{"begin":126,"end":131},"obj":"CD"},{"id":"T4695","span":{"begin":132,"end":134},"obj":"TO"},{"id":"T4696","span":{"begin":135,"end":144},"obj":"VB"},{"id":"T4697","span":{"begin":145,"end":147},"obj":"IN"},{"id":"T4698","span":{"begin":148,"end":153},"obj":"NNP"},{"id":"T4699","span":{"begin":154,"end":161},"obj":"VBD"},{"id":"T4700","span":{"begin":162,"end":167},"obj":"NNP"},{"id":"T4701","span":{"begin":168,"end":171},"obj":"NNP"},{"id":"T4702","span":{"begin":172,"end":179},"obj":"JJ"},{"id":"T4703","span":{"begin":180,"end":182},"obj":"IN"},{"id":"T4704","span":{"begin":183,"end":188},"obj":"JJ"},{"id":"T4705","span":{"begin":189,"end":200},"obj":"NNS"},{"id":"T4706","span":{"begin":200,"end":201},"obj":","},{"id":"T4707","span":{"begin":202,"end":204},"obj":"PRP"},{"id":"T4708","span":{"begin":205,"end":214},"obj":"VBD"},{"id":"T4709","span":{"begin":215,"end":219},"obj":"NNP"},{"id":"T4710","span":{"begin":220,"end":228},"obj":"NN"},{"id":"T4711","span":{"begin":229,"end":231},"obj":"IN"},{"id":"T4712","span":{"begin":232,"end":239},"obj":"JJ"},{"id":"T4713","span":{"begin":240,"end":247},"obj":"NNS"},{"id":"T4714","span":{"begin":248,"end":252},"obj":"IN"},{"id":"T4715","span":{"begin":253,"end":266},"obj":"JJ"},{"id":"T4716","span":{"begin":267,"end":271},"obj":"NNS"},{"id":"T4717","span":{"begin":271,"end":272},"obj":"."},{"id":"T4718","span":{"begin":273,"end":280},"obj":"JJ"},{"id":"T4719","span":{"begin":281,"end":283},"obj":"TO"},{"id":"T4720","span":{"begin":284,"end":292},"obj":"JJ"},{"id":"T4721","span":{"begin":293,"end":300},"obj":"NNS"},{"id":"T4722","span":{"begin":301,"end":302},"obj":"NNP"},{"id":"T4723","span":{"begin":302,"end":304},"obj":"CD"},{"id":"T4724","span":{"begin":304,"end":305},"obj":"NNP"},{"id":"T4725","span":{"begin":305,"end":306},"obj":","},{"id":"T4726","span":{"begin":307,"end":314},"obj":"JJ"},{"id":"T4727","span":{"begin":315,"end":320},"obj":"NN"},{"id":"T4728","span":{"begin":321,"end":335},"obj":"RB"},{"id":"T4729","span":{"begin":336,"end":341},"obj":"VBN"},{"id":"T4730","span":{"begin":342,"end":345},"obj":"NN"},{"id":"T4731","span":{"begin":346,"end":348},"obj":"IN"},{"id":"T4732","span":{"begin":349,"end":363},"obj":"JJ"},{"id":"T4733","span":{"begin":364,"end":373},"obj":"NNS"},{"id":"T4734","span":{"begin":374,"end":375},"obj":"-LRB-"},{"id":"T4735","span":{"begin":375,"end":381},"obj":"NN"},{"id":"T4736","span":{"begin":382,"end":384},"obj":"CD"},{"id":"T4737","span":{"begin":384,"end":385},"obj":"-RRB-"},{"id":"T4738","span":{"begin":385,"end":386},"obj":"."},{"id":"T4739","span":{"begin":387,"end":400},"obj":"RB"},{"id":"T4740","span":{"begin":400,"end":401},"obj":","},{"id":"T4741","span":{"begin":402,"end":408},"obj":"VBG"},{"id":"T4742","span":{"begin":409,"end":414},"obj":"NNP"},{"id":"T4743","span":{"begin":415,"end":418},"obj":"VBD"},{"id":"T4744","span":{"begin":419,"end":422},"obj":"RB"},{"id":"T4745","span":{"begin":423,"end":428},"obj":"VB"},{"id":"T4746","span":{"begin":429,"end":434},"obj":"NNP"},{"id":"T4747","span":{"begin":435,"end":438},"obj":"NNP"},{"id":"T4748","span":{"begin":439,"end":446},"obj":"JJ"},{"id":"T4749","span":{"begin":447,"end":449},"obj":"IN"},{"id":"T4750","span":{"begin":450,"end":454},"obj":"NNP"},{"id":"T4751","span":{"begin":454,"end":455},"obj":"."},{"id":"T4752","span":{"begin":456,"end":458},"obj":"IN"},{"id":"T4753","span":{"begin":459,"end":467},"obj":"NN"},{"id":"T4754","span":{"begin":467,"end":468},"obj":","},{"id":"T4755","span":{"begin":469,"end":474},"obj":"IN"},{"id":"T4756","span":{"begin":475,"end":486},"obj":"RB"},{"id":"T4757","span":{"begin":487,"end":499},"obj":"VBG"},{"id":"T4758","span":{"begin":500,"end":505},"obj":"JJ"},{"id":"T4759","span":{"begin":506,"end":510},"obj":"NNS"},{"id":"T4760","span":{"begin":511,"end":515},"obj":"IN"},{"id":"T4761","span":{"begin":516,"end":527},"obj":"JJ"},{"id":"T4762","span":{"begin":528,"end":538},"obj":"NNS"},{"id":"T4763","span":{"begin":539,"end":549},"obj":"VBG"},{"id":"T4764","span":{"begin":550,"end":554},"obj":"CD"},{"id":"T4765","span":{"begin":555,"end":560},"obj":"JJ"},{"id":"T4766","span":{"begin":561,"end":570},"obj":"NN"},{"id":"T4767","span":{"begin":571,"end":578},"obj":"JJ"},{"id":"T4768","span":{"begin":579,"end":588},"obj":"NNS"},{"id":"T4769","span":{"begin":588,"end":589},"obj":","},{"id":"T4770","span":{"begin":590,"end":595},"obj":"NNP"},{"id":"T4771","span":{"begin":596,"end":605},"obj":"NN"},{"id":"T4772","span":{"begin":606,"end":608},"obj":"IN"},{"id":"T4773","span":{"begin":609,"end":612},"obj":"DT"},{"id":"T4774","span":{"begin":613,"end":624},"obj":"VBN"},{"id":"T4775","span":{"begin":625,"end":630},"obj":"NNS"},{"id":"T4776","span":{"begin":631,"end":639},"obj":"VBD"},{"id":"T4777","span":{"begin":640,"end":642},"obj":"IN"},{"id":"T4778","span":{"begin":643,"end":644},"obj":"DT"},{"id":"T4779","span":{"begin":645,"end":653},"obj":"JJ"},{"id":"T4780","span":{"begin":654,"end":662},"obj":"NN"},{"id":"T4781","span":{"begin":663,"end":665},"obj":"IN"},{"id":"T4782","span":{"begin":666,"end":670},"obj":"NNP"},{"id":"T4783","span":{"begin":671,"end":678},"obj":"NN"},{"id":"T4784","span":{"begin":679,"end":681},"obj":"IN"},{"id":"T4785","span":{"begin":682,"end":685},"obj":"DT"},{"id":"T4786","span":{"begin":686,"end":693},"obj":"NN"},{"id":"T4787","span":{"begin":694,"end":699},"obj":"NNS"},{"id":"T4788","span":{"begin":700,"end":708},"obj":"VBN"},{"id":"T4789","span":{"begin":709,"end":711},"obj":"TO"},{"id":"T4790","span":{"begin":712,"end":715},"obj":"NNP"},{"id":"T4791","span":{"begin":716,"end":717},"obj":"-LRB-"},{"id":"T4792","span":{"begin":717,"end":724},"obj":"NN"},{"id":"T4793","span":{"begin":724,"end":725},"obj":"-RRB-"},{"id":"T4794","span":{"begin":725,"end":726},"obj":":"},{"id":"T4795","span":{"begin":726,"end":733},"obj":"VBN"},{"id":"T4796","span":{"begin":734,"end":745},"obj":"VBN"},{"id":"T4797","span":{"begin":746,"end":750},"obj":"NNS"},{"id":"T4798","span":{"begin":751,"end":752},"obj":"-LRB-"},{"id":"T4799","span":{"begin":752,"end":758},"obj":"NN"},{"id":"T4800","span":{"begin":759,"end":761},"obj":"NN"},{"id":"T4801","span":{"begin":761,"end":762},"obj":"-RRB-"},{"id":"T4802","span":{"begin":762,"end":763},"obj":"."},{"id":"T4803","span":{"begin":764,"end":766},"obj":"IN"},{"id":"T4804","span":{"begin":767,"end":768},"obj":"DT"},{"id":"T4805","span":{"begin":769,"end":776},"obj":"NN"},{"id":"T4806","span":{"begin":776,"end":777},"obj":","},{"id":"T4807","span":{"begin":778,"end":781},"obj":"DT"},{"id":"T4808","span":{"begin":782,"end":791},"obj":"NNP"},{"id":"T4809","span":{"begin":792,"end":801},"obj":"VB"},{"id":"T4810","span":{"begin":802,"end":809},"obj":"VBG"},{"id":"T4811","span":{"begin":810,"end":815},"obj":"JJ"},{"id":"T4812","span":{"begin":816,"end":823},"obj":"JJ"},{"id":"T4813","span":{"begin":824,"end":829},"obj":"NNS"},{"id":"T4814","span":{"begin":830,"end":833},"obj":"VBD"},{"id":"T4815","span":{"begin":834,"end":838},"obj":"VBN"},{"id":"T4816","span":{"begin":838,"end":839},"obj":"."},{"id":"T4817","span":{"begin":840,"end":845},"obj":"NNS"},{"id":"T4818","span":{"begin":846,"end":857},"obj":"VBN"},{"id":"T4819","span":{"begin":858,"end":862},"obj":"IN"},{"id":"T4820","span":{"begin":863,"end":866},"obj":"DT"},{"id":"T4821","span":{"begin":867,"end":876},"obj":"NNP"},{"id":"T4822","span":{"begin":877,"end":886},"obj":"VBP"},{"id":"T4823","span":{"begin":887,"end":890},"obj":"VBD"},{"id":"T4824","span":{"begin":891,"end":894},"obj":"RB"},{"id":"T4825","span":{"begin":895,"end":902},"obj":"VB"},{"id":"T4826","span":{"begin":903,"end":907},"obj":"NNP"},{"id":"T4827","span":{"begin":908,"end":910},"obj":"IN"},{"id":"T4828","span":{"begin":911,"end":914},"obj":"DT"},{"id":"T4829","span":{"begin":915,"end":922},"obj":"NN"},{"id":"T4830","span":{"begin":923,"end":925},"obj":"CC"},{"id":"T4831","span":{"begin":926,"end":934},"obj":"NN"},{"id":"T4832","span":{"begin":935,"end":937},"obj":"IN"},{"id":"T4833","span":{"begin":938,"end":943},"obj":"NNP"},{"id":"T4834","span":{"begin":944,"end":945},"obj":"-LRB-"},{"id":"T4835","span":{"begin":945,"end":951},"obj":"NNP"},{"id":"T4836","span":{"begin":952,"end":954},"obj":"NNP"},{"id":"T4837","span":{"begin":954,"end":955},"obj":"-RRB-"},{"id":"T4838","span":{"begin":955,"end":956},"obj":"."},{"id":"T4839","span":{"begin":1987,"end":1989},"obj":"PRP"},{"id":"T4840","span":{"begin":1990,"end":1994},"obj":"JJ"},{"id":"T4841","span":{"begin":1995,"end":2007},"obj":"VBD"},{"id":"T4842","span":{"begin":2008,"end":2015},"obj":"IN"},{"id":"T4843","span":{"begin":2016,"end":2021},"obj":"NNP"},{"id":"T4844","span":{"begin":2022,"end":2029},"obj":"VBD"},{"id":"T4845","span":{"begin":2030,"end":2035},"obj":"JJ"},{"id":"T4846","span":{"begin":2036,"end":2044},"obj":"NN"},{"id":"T4847","span":{"begin":2045,"end":2047},"obj":"IN"},{"id":"T4848","span":{"begin":2048,"end":2051},"obj":"DT"},{"id":"T4849","span":{"begin":2052,"end":2057},"obj":"NN"},{"id":"T4850","span":{"begin":2058,"end":2068},"obj":"NN"},{"id":"T4851","span":{"begin":2069,"end":2073},"obj":"NN"},{"id":"T4852","span":{"begin":2074,"end":2078},"obj":"NN"},{"id":"T4853","span":{"begin":2078,"end":2079},"obj":","},{"id":"T4854","span":{"begin":2080,"end":2083},"obj":"NNP"},{"id":"T4855","span":{"begin":2084,"end":2089},"obj":"CD"},{"id":"T4856","span":{"begin":2089,"end":2090},"obj":"."},{"id":"T4857","span":{"begin":2091,"end":2094},"obj":"NNP"},{"id":"T4858","span":{"begin":2095,"end":2100},"obj":"CD"},{"id":"T4859","span":{"begin":2101,"end":2106},"obj":"NNS"},{"id":"T4860","span":{"begin":2107,"end":2111},"obj":"VBD"},{"id":"T4861","span":{"begin":2112,"end":2123},"obj":"VBN"},{"id":"T4862","span":{"begin":2124,"end":2128},"obj":"IN"},{"id":"T4863","span":{"begin":2129,"end":2135},"obj":"CC"},{"id":"T4864","span":{"begin":2136,"end":2139},"obj":"DT"},{"id":"T4865","span":{"begin":2140,"end":2150},"obj":"NNP"},{"id":"T4866","span":{"begin":2151,"end":2159},"obj":"NN"},{"id":"T4867","span":{"begin":2160,"end":2162},"obj":"CC"},{"id":"T4868","span":{"begin":2163,"end":2170},"obj":"NN"},{"id":"T4869","span":{"begin":2171,"end":2180},"obj":"JJ"},{"id":"T4870","span":{"begin":2181,"end":2190},"obj":"VBP"},{"id":"T4871","span":{"begin":2190,"end":2191},"obj":"."},{"id":"T4872","span":{"begin":2192,"end":2194},"obj":"IN"},{"id":"T4873","span":{"begin":2195,"end":2200},"obj":"VBN"},{"id":"T4874","span":{"begin":2201,"end":2203},"obj":"IN"},{"id":"T4875","span":{"begin":2204,"end":2210},"obj":"NN"},{"id":"T4876","span":{"begin":2211,"end":2213},"obj":"CD"},{"id":"T4877","span":{"begin":2213,"end":2214},"obj":","},{"id":"T4878","span":{"begin":2215,"end":2220},"obj":"NNP"},{"id":"T4879","span":{"begin":2221,"end":2230},"obj":"NN"},{"id":"T4880","span":{"begin":2231,"end":2233},"obj":"IN"},{"id":"T4881","span":{"begin":2234,"end":2237},"obj":"NNP"},{"id":"T4882","span":{"begin":2238,"end":2243},"obj":"CD"},{"id":"T4883","span":{"begin":2244,"end":2249},"obj":"NNS"},{"id":"T4884","span":{"begin":2250,"end":2259},"obj":"VBD"},{"id":"T4885","span":{"begin":2260,"end":2265},"obj":"JJ"},{"id":"T4886","span":{"begin":2266,"end":2274},"obj":"NN"},{"id":"T4887","span":{"begin":2275,"end":2283},"obj":"NN"},{"id":"T4888","span":{"begin":2284,"end":2286},"obj":"IN"},{"id":"T4889","span":{"begin":2287,"end":2295},"obj":"CD"},{"id":"T4890","span":{"begin":2296,"end":2300},"obj":"IN"},{"id":"T4891","span":{"begin":2301,"end":2305},"obj":"DT"},{"id":"T4892","span":{"begin":2306,"end":2308},"obj":"IN"},{"id":"T4893","span":{"begin":2309,"end":2320},"obj":"JJ"},{"id":"T4894","span":{"begin":2321,"end":2326},"obj":"NNS"},{"id":"T4895","span":{"begin":2326,"end":2327},"obj":"."},{"id":"T4896","span":{"begin":2328,"end":2336},"obj":"RB"},{"id":"T4897","span":{"begin":2336,"end":2337},"obj":","},{"id":"T4898","span":{"begin":2338,"end":2341},"obj":"PRP$"},{"id":"T4899","span":{"begin":2342,"end":2346},"obj":"NNS"},{"id":"T4900","span":{"begin":2347,"end":2358},"obj":"VBP"},{"id":"T4901","span":{"begin":2359,"end":2363},"obj":"IN"},{"id":"T4902","span":{"begin":2364,"end":2369},"obj":"NNP"},{"id":"T4903","span":{"begin":2370,"end":2377},"obj":"VBD"},{"id":"T4904","span":{"begin":2378,"end":2383},"obj":"NNP"},{"id":"T4905","span":{"begin":2384,"end":2399},"obj":"JJ"},{"id":"T4906","span":{"begin":2400,"end":2408},"obj":"NN"},{"id":"T4907","span":{"begin":2409,"end":2411},"obj":"IN"},{"id":"T4908","span":{"begin":2412,"end":2423},"obj":"NNS"},{"id":"T4909","span":{"begin":2423,"end":2424},"obj":"."},{"id":"T22818","span":{"begin":1001,"end":1006},"obj":"NNP"},{"id":"T22819","span":{"begin":1007,"end":1014},"obj":"VBZ"},{"id":"T22820","span":{"begin":1015,"end":1020},"obj":"JJ"},{"id":"T22821","span":{"begin":1021,"end":1036},"obj":"JJ"},{"id":"T22822","span":{"begin":1037,"end":1045},"obj":"NN"},{"id":"T22823","span":{"begin":1046,"end":1048},"obj":"IN"},{"id":"T22824","span":{"begin":1049,"end":1060},"obj":"NNS"},{"id":"T22825","span":{"begin":1060,"end":1061},"obj":"."},{"id":"T22826","span":{"begin":1062,"end":1063},"obj":"-LRB-"},{"id":"T22827","span":{"begin":1063,"end":1064},"obj":"NNP"},{"id":"T22828","span":{"begin":1064,"end":1065},"obj":"-RRB-"},{"id":"T22829","span":{"begin":1066,"end":1072},"obj":"NNP"},{"id":"T22830","span":{"begin":1073,"end":1077},"obj":"VBD"},{"id":"T22831","span":{"begin":1078,"end":1087},"obj":"VBN"},{"id":"T22832","span":{"begin":1088,"end":1092},"obj":"IN"},{"id":"T22833","span":{"begin":1093,"end":1098},"obj":"JJ"},{"id":"T22834","span":{"begin":1099,"end":1103},"obj":"NNS"},{"id":"T22835","span":{"begin":1104,"end":1111},"obj":"VBN"},{"id":"T22836","span":{"begin":1112,"end":1119},"obj":"IN"},{"id":"T22837","span":{"begin":1120,"end":1122},"obj":"CC"},{"id":"T22838","span":{"begin":1123,"end":1127},"obj":"IN"},{"id":"T22839","span":{"begin":1128,"end":1133},"obj":"NNP"},{"id":"T22840","span":{"begin":1134,"end":1137},"obj":"IN"},{"id":"T22841","span":{"begin":1138,"end":1140},"obj":"CD"},{"id":"T22842","span":{"begin":1141,"end":1143},"obj":"CC"},{"id":"T22843","span":{"begin":1144,"end":1146},"obj":"CD"},{"id":"T22844","span":{"begin":1147,"end":1154},"obj":"NNS"},{"id":"T22845","span":{"begin":1154,"end":1155},"obj":"."},{"id":"T22846","span":{"begin":1156,"end":1161},"obj":"NNP"},{"id":"T22847","span":{"begin":1162,"end":1165},"obj":"NNP"},{"id":"T22848","span":{"begin":1166,"end":1173},"obj":"JJ"},{"id":"T22849","span":{"begin":1174,"end":1182},"obj":"NN"},{"id":"T22850","span":{"begin":1183,"end":1186},"obj":"VBD"},{"id":"T22851","span":{"begin":1187,"end":1195},"obj":"VBN"},{"id":"T22852","span":{"begin":1196,"end":1198},"obj":"IN"},{"id":"T22853","span":{"begin":1199,"end":1203},"obj":"NNP"},{"id":"T22854","span":{"begin":1204,"end":1209},"obj":"NNP"},{"id":"T22855","span":{"begin":1210,"end":1212},"obj":"VBZ"},{"id":"T22856","span":{"begin":1213,"end":1214},"obj":"DT"},{"id":"T22857","span":{"begin":1215,"end":1229},"obj":"JJ"},{"id":"T22858","span":{"begin":1230,"end":1234},"obj":"NN"},{"id":"T22859","span":{"begin":1235,"end":1239},"obj":"IN"},{"id":"T22860","span":{"begin":1240,"end":1245},"obj":"CD"},{"id":"T22861","span":{"begin":1246,"end":1257},"obj":"JJ"},{"id":"T22862","span":{"begin":1258,"end":1269},"obj":"NNS"},{"id":"T22863","span":{"begin":1269,"end":1270},"obj":"."},{"id":"T22864","span":{"begin":1271,"end":1273},"obj":"NNP"},{"id":"T22865","span":{"begin":1273,"end":1274},"obj":":"},{"id":"T22866","span":{"begin":1275,"end":1289},"obj":"JJ"},{"id":"T22867","span":{"begin":1289,"end":1290},"obj":"."},{"id":"T22868","span":{"begin":1291,"end":1292},"obj":"-LRB-"},{"id":"T22869","span":{"begin":1292,"end":1293},"obj":"NNP"},{"id":"T22870","span":{"begin":1293,"end":1294},"obj":"-RRB-"},{"id":"T22871","span":{"begin":1295,"end":1300},"obj":"NNP"},{"id":"T22872","span":{"begin":1301,"end":1305},"obj":"NNP"},{"id":"T22873","span":{"begin":1306,"end":1317},"obj":"RB"},{"id":"T22874","span":{"begin":1318,"end":1329},"obj":"VBD"},{"id":"T22875","span":{"begin":1330,"end":1334},"obj":"IN"},{"id":"T22876","span":{"begin":1335,"end":1344},"obj":"JJ"},{"id":"T22877","span":{"begin":1345,"end":1347},"obj":"CC"},{"id":"T22878","span":{"begin":1348,"end":1359},"obj":"JJ"},{"id":"T22879","span":{"begin":1360,"end":1370},"obj":"NNS"},{"id":"T22880","span":{"begin":1371,"end":1375},"obj":"VBD"},{"id":"T22881","span":{"begin":1376,"end":1383},"obj":"VBN"},{"id":"T22882","span":{"begin":1384,"end":1388},"obj":"IN"},{"id":"T22883","span":{"begin":1389,"end":1394},"obj":"NNP"},{"id":"T22884","span":{"begin":1395,"end":1397},"obj":"IN"},{"id":"T22885","span":{"begin":1398,"end":1404},"obj":"NNP"},{"id":"T22886","span":{"begin":1405,"end":1411},"obj":"NN"},{"id":"T22887","span":{"begin":1412,"end":1415},"obj":"CC"},{"id":"T22888","span":{"begin":1416,"end":1425},"obj":"VBN"},{"id":"T22889","span":{"begin":1426,"end":1429},"obj":"IN"},{"id":"T22890","span":{"begin":1430,"end":1431},"obj":"CD"},{"id":"T22891","span":{"begin":1432,"end":1437},"obj":"NNS"},{"id":"T22892","span":{"begin":1438,"end":1444},"obj":"IN"},{"id":"T22893","span":{"begin":1445,"end":1448},"obj":"DT"},{"id":"T22894","span":{"begin":1449,"end":1459},"obj":"NN"},{"id":"T22895","span":{"begin":1460,"end":1462},"obj":"IN"},{"id":"T22896","span":{"begin":1463,"end":1469},"obj":"NN"},{"id":"T22897","span":{"begin":1469,"end":1470},"obj":"."},{"id":"T22898","span":{"begin":1471,"end":1476},"obj":"JJ"},{"id":"T22899","span":{"begin":1477,"end":1485},"obj":"NN"},{"id":"T22900","span":{"begin":1486,"end":1489},"obj":"VBD"},{"id":"T22901","span":{"begin":1490,"end":1498},"obj":"VBN"},{"id":"T22902","span":{"begin":1499,"end":1501},"obj":"IN"},{"id":"T22903","span":{"begin":1502,"end":1511},"obj":"VBG"},{"id":"T22904","span":{"begin":1512,"end":1515},"obj":"DT"},{"id":"T22905","span":{"begin":1516,"end":1522},"obj":"NN"},{"id":"T22906","span":{"begin":1523,"end":1525},"obj":"IN"},{"id":"T22907","span":{"begin":1526,"end":1530},"obj":"NNP"},{"id":"T22908","span":{"begin":1531,"end":1539},"obj":"VBD"},{"id":"T22909","span":{"begin":1540,"end":1544},"obj":"IN"},{"id":"T22910","span":{"begin":1545,"end":1548},"obj":"DT"},{"id":"T22911","span":{"begin":1549,"end":1555},"obj":"NN"},{"id":"T22912","span":{"begin":1556,"end":1559},"obj":"CC"},{"id":"T22913","span":{"begin":1560,"end":1564},"obj":"NNS"},{"id":"T22914","span":{"begin":1565,"end":1568},"obj":"VBP"},{"id":"T22915","span":{"begin":1569,"end":1578},"obj":"VBN"},{"id":"T22916","span":{"begin":1579,"end":1581},"obj":"IN"},{"id":"T22917","span":{"begin":1582,"end":1586},"obj":"VBP"},{"id":"T22918","span":{"begin":1587,"end":1595},"obj":"NN"},{"id":"T22919","span":{"begin":1596,"end":1598},"obj":"IN"},{"id":"T22920","span":{"begin":1599,"end":1603},"obj":"NNP"},{"id":"T22921","span":{"begin":1604,"end":1612},"obj":"NN"},{"id":"T22922","span":{"begin":1613,"end":1617},"obj":"IN"},{"id":"T22923","span":{"begin":1618,"end":1622},"obj":"DT"},{"id":"T22924","span":{"begin":1623,"end":1625},"obj":"IN"},{"id":"T22925","span":{"begin":1626,"end":1635},"obj":"JJ"},{"id":"T22926","span":{"begin":1636,"end":1647},"obj":"VBN"},{"id":"T22927","span":{"begin":1648,"end":1655},"obj":"VBG"},{"id":"T22928","span":{"begin":1656,"end":1661},"obj":"NNS"},{"id":"T22929","span":{"begin":1661,"end":1662},"obj":"."},{"id":"T22930","span":{"begin":1663,"end":1664},"obj":"-LRB-"},{"id":"T22931","span":{"begin":1664,"end":1665},"obj":"NNP"},{"id":"T22932","span":{"begin":1665,"end":1666},"obj":"-RRB-"},{"id":"T22933","span":{"begin":1667,"end":1670},"obj":"NNP"},{"id":"T22934","span":{"begin":1671,"end":1676},"obj":"CD"},{"id":"T22935","span":{"begin":1677,"end":1682},"obj":"NNS"},{"id":"T22936","span":{"begin":1683,"end":1687},"obj":"VBD"},{"id":"T22937","span":{"begin":1688,"end":1699},"obj":"RB"},{"id":"T22938","span":{"begin":1700,"end":1711},"obj":"VBN"},{"id":"T22939","span":{"begin":1712,"end":1716},"obj":"IN"},{"id":"T22940","span":{"begin":1717,"end":1726},"obj":"NNP"},{"id":"T22941","span":{"begin":1727,"end":1729},"obj":"CC"},{"id":"T22942","span":{"begin":1730,"end":1741},"obj":"NNP"},{"id":"T22943","span":{"begin":1742,"end":1751},"obj":"VBP"},{"id":"T22944","span":{"begin":1751,"end":1752},"obj":"."},{"id":"T22945","span":{"begin":1753,"end":1758},"obj":"NNS"},{"id":"T22946","span":{"begin":1759,"end":1763},"obj":"VBD"},{"id":"T22947","span":{"begin":1764,"end":1769},"obj":"JJ"},{"id":"T22948","span":{"begin":1770,"end":1777},"obj":"VBN"},{"id":"T22949","span":{"begin":1778,"end":1781},"obj":"IN"},{"id":"T22950","span":{"begin":1782,"end":1783},"obj":"CD"},{"id":"T22951","span":{"begin":1784,"end":1789},"obj":"NNS"},{"id":"T22952","span":{"begin":1790,"end":1795},"obj":"RB"},{"id":"T22953","span":{"begin":1796,"end":1798},"obj":"TO"},{"id":"T22954","span":{"begin":1799,"end":1800},"obj":"CD"},{"id":"T22955","span":{"begin":1801,"end":1806},"obj":"NNS"},{"id":"T22956","span":{"begin":1807,"end":1818},"obj":"NN"},{"id":"T22957","span":{"begin":1819,"end":1823},"obj":"IN"},{"id":"T22958","span":{"begin":1824,"end":1829},"obj":"NN"},{"id":"T22959","span":{"begin":1830,"end":1841},"obj":"JJ"},{"id":"T22960","span":{"begin":1842,"end":1847},"obj":"NNP"},{"id":"T22961","span":{"begin":1848,"end":1849},"obj":"-LRB-"},{"id":"T22962","span":{"begin":1849,"end":1852},"obj":"CD"},{"id":"T22963","span":{"begin":1853,"end":1858},"obj":"NN"},{"id":"T22964","span":{"begin":1858,"end":1859},"obj":"-RRB-"},{"id":"T22965","span":{"begin":1859,"end":1860},"obj":"."},{"id":"T22966","span":{"begin":1861,"end":1868},"obj":"NNP"},{"id":"T22967","span":{"begin":1869,"end":1874},"obj":"NNS"},{"id":"T22968","span":{"begin":1875,"end":1878},"obj":"VBD"},{"id":"T22969","span":{"begin":1879,"end":1888},"obj":"VBN"},{"id":"T22970","span":{"begin":1889,"end":1891},"obj":"TO"},{"id":"T22971","span":{"begin":1892,"end":1899},"obj":"VB"},{"id":"T22972","span":{"begin":1900,"end":1904},"obj":"NNP"},{"id":"T22973","span":{"begin":1905,"end":1915},"obj":"NN"},{"id":"T22974","span":{"begin":1915,"end":1916},"obj":"."},{"id":"T22975","span":{"begin":1917,"end":1921},"obj":"NNS"},{"id":"T22976","span":{"begin":1922,"end":1925},"obj":"VBP"},{"id":"T22977","span":{"begin":1926,"end":1935},"obj":"VBN"},{"id":"T22978","span":{"begin":1936,"end":1940},"obj":"JJ"},{"id":"T22979","span":{"begin":1941,"end":1942},"obj":"NN"},{"id":"T22980","span":{"begin":1943,"end":1948},"obj":"NNP"},{"id":"T22981","span":{"begin":1948,"end":1949},"obj":","},{"id":"T22982","span":{"begin":1950,"end":1953},"obj":"IN"},{"id":"T22983","span":{"begin":1954,"end":1959},"obj":"CD"},{"id":"T22984","span":{"begin":1960,"end":1971},"obj":"JJ"},{"id":"T22985","span":{"begin":1972,"end":1983},"obj":"NNS"},{"id":"T22986","span":{"begin":1983,"end":1984},"obj":"."}],"relations":[{"id":"R3429","pred":"nsubj","subj":"T4674","obj":"T4675"},{"id":"R3430","pred":"ROOT","subj":"T4675","obj":"T4675"},{"id":"R3431","pred":"compound","subj":"T4676","obj":"T4678"},{"id":"R3432","pred":"compound","subj":"T4677","obj":"T4678"},{"id":"R3433","pred":"dobj","subj":"T4678","obj":"T4675"},{"id":"R3434","pred":"prep","subj":"T4679","obj":"T4678"},{"id":"R3435","pred":"compound","subj":"T4680","obj":"T4681"},{"id":"R3436","pred":"pobj","subj":"T4681","obj":"T4679"},{"id":"R3437","pred":"compound","subj":"T4682","obj":"T4683"},{"id":"R3438","pred":"conj","subj":"T4683","obj":"T4678"},{"id":"R3439","pred":"punct","subj":"T4684","obj":"T4685"},{"id":"R3440","pred":"appos","subj":"T4685","obj":"T4683"},{"id":"R3441","pred":"punct","subj":"T4686","obj":"T4685"},{"id":"R3442","pred":"cc","subj":"T4687","obj":"T4683"},{"id":"R3443","pred":"compound","subj":"T4688","obj":"T4691"},{"id":"R3444","pred":"compound","subj":"T4689","obj":"T4691"},{"id":"R3445","pred":"compound","subj":"T4690","obj":"T4691"},{"id":"R3446","pred":"conj","subj":"T4691","obj":"T4683"},{"id":"R3447","pred":"punct","subj":"T4692","obj":"T4691"},{"id":"R3448","pred":"conj","subj":"T4693","obj":"T4691"},{"id":"R3449","pred":"nummod","subj":"T4694","obj":"T4693"},{"id":"R3450","pred":"aux","subj":"T4695","obj":"T4696"},{"id":"R3451","pred":"advcl","subj":"T4696","obj":"T4675"},{"id":"R3452","pred":"mark","subj":"T4697","obj":"T4699"},{"id":"R3453","pred":"nsubj","subj":"T4698","obj":"T4699"},{"id":"R3454","pred":"advcl","subj":"T4699","obj":"T4708"},{"id":"R3455","pred":"compound","subj":"T4700","obj":"T4701"},{"id":"R3456","pred":"dobj","subj":"T4701","obj":"T4699"},{"id":"R3457","pred":"advcl","subj":"T4702","obj":"T4699"},{"id":"R3458","pred":"prep","subj":"T4703","obj":"T4702"},{"id":"R3459","pred":"amod","subj":"T4704","obj":"T4705"},{"id":"R3460","pred":"pobj","subj":"T4705","obj":"T4703"},{"id":"R3461","pred":"punct","subj":"T4706","obj":"T4708"},{"id":"R3462","pred":"nsubj","subj":"T4707","obj":"T4708"},{"id":"R3463","pred":"conj","subj":"T4708","obj":"T4675"},{"id":"R3464","pred":"compound","subj":"T4709","obj":"T4710"},{"id":"R3465","pred":"dobj","subj":"T4710","obj":"T4708"},{"id":"R3466","pred":"prep","subj":"T4711","obj":"T4708"},{"id":"R3467","pred":"amod","subj":"T4712","obj":"T4713"},{"id":"R3468","pred":"pobj","subj":"T4713","obj":"T4711"},{"id":"R3469","pred":"prep","subj":"T4714","obj":"T4713"},{"id":"R3470","pred":"amod","subj":"T4715","obj":"T4716"},{"id":"R3471","pred":"pobj","subj":"T4716","obj":"T4714"},{"id":"R3472","pred":"punct","subj":"T4717","obj":"T4708"},{"id":"R3473","pred":"ROOT","subj":"T4718","obj":"T4718"},{"id":"R3474","pred":"prep","subj":"T4719","obj":"T4718"},{"id":"R3475","pred":"amod","subj":"T4720","obj":"T4721"},{"id":"R3476","pred":"pobj","subj":"T4721","obj":"T4719"},{"id":"R3477","pred":"compound","subj":"T4722","obj":"T4724"},{"id":"R3478","pred":"compound","subj":"T4723","obj":"T4724"},{"id":"R3479","pred":"appos","subj":"T4724","obj":"T4718"},{"id":"R3480","pred":"punct","subj":"T4725","obj":"T4724"},{"id":"R3481","pred":"amod","subj":"T4726","obj":"T4727"},{"id":"R3482","pred":"appos","subj":"T4727","obj":"T4724"},{"id":"R3483","pred":"advmod","subj":"T4728","obj":"T4729"},{"id":"R3484","pred":"amod","subj":"T4729","obj":"T4730"},{"id":"R3485","pred":"conj","subj":"T4730","obj":"T4724"},{"id":"R3486","pred":"prep","subj":"T4731","obj":"T4730"},{"id":"R3487","pred":"amod","subj":"T4732","obj":"T4733"},{"id":"R3488","pred":"pobj","subj":"T4733","obj":"T4731"},{"id":"R3489","pred":"punct","subj":"T4734","obj":"T4735"},{"id":"R3490","pred":"parataxis","subj":"T4735","obj":"T4733"},{"id":"R3491","pred":"nummod","subj":"T4736","obj":"T4735"},{"id":"R3492","pred":"punct","subj":"T4737","obj":"T4735"},{"id":"R3493","pred":"punct","subj":"T4738","obj":"T4718"},{"id":"R3494","pred":"advmod","subj":"T4739","obj":"T4745"},{"id":"R3495","pred":"punct","subj":"T4740","obj":"T4745"},{"id":"R3496","pred":"advcl","subj":"T4741","obj":"T4745"},{"id":"R3497","pred":"dobj","subj":"T4742","obj":"T4741"},{"id":"R3498","pred":"aux","subj":"T4743","obj":"T4745"},{"id":"R3499","pred":"neg","subj":"T4744","obj":"T4745"},{"id":"R3500","pred":"ROOT","subj":"T4745","obj":"T4745"},{"id":"R3501","pred":"compound","subj":"T4746","obj":"T4747"},{"id":"R3502","pred":"dobj","subj":"T4747","obj":"T4745"},{"id":"R3503","pred":"acl","subj":"T4748","obj":"T4747"},{"id":"R3504","pred":"agent","subj":"T4749","obj":"T4748"},{"id":"R3505","pred":"pobj","subj":"T4750","obj":"T4749"},{"id":"R3506","pred":"punct","subj":"T4751","obj":"T4745"},{"id":"R3507","pred":"prep","subj":"T4752","obj":"T4776"},{"id":"R3508","pred":"pobj","subj":"T4753","obj":"T4752"},{"id":"R3509","pred":"punct","subj":"T4754","obj":"T4776"},{"id":"R3510","pred":"prep","subj":"T4755","obj":"T4776"},{"id":"R3511","pred":"advmod","subj":"T4756","obj":"T4757"},{"id":"R3512","pred":"pcomp","subj":"T4757","obj":"T4755"},{"id":"R3513","pred":"amod","subj":"T4758","obj":"T4759"},{"id":"R3514","pred":"dobj","subj":"T4759","obj":"T4757"},{"id":"R3515","pred":"prep","subj":"T4760","obj":"T4757"},{"id":"R3516","pred":"compound","subj":"T4761","obj":"T4762"},{"id":"R3517","pred":"pobj","subj":"T4762","obj":"T4760"},{"id":"R3518","pred":"acl","subj":"T4763","obj":"T4762"},{"id":"R3519","pred":"nummod","subj":"T4764","obj":"T4768"},{"id":"R3520","pred":"amod","subj":"T4765","obj":"T4768"},{"id":"R3521","pred":"amod","subj":"T4766","obj":"T4768"},{"id":"R3522","pred":"amod","subj":"T4767","obj":"T4768"},{"id":"R3523","pred":"dobj","subj":"T4768","obj":"T4763"},{"id":"R3524","pred":"punct","subj":"T4769","obj":"T4768"},{"id":"R3525","pred":"compound","subj":"T4770","obj":"T4771"},{"id":"R3526","pred":"nsubj","subj":"T4771","obj":"T4776"},{"id":"R3527","pred":"prep","subj":"T4772","obj":"T4771"},{"id":"R3528","pred":"det","subj":"T4773","obj":"T4775"},{"id":"R3529","pred":"amod","subj":"T4774","obj":"T4775"},{"id":"R3530","pred":"pobj","subj":"T4775","obj":"T4772"},{"id":"R3531","pred":"ROOT","subj":"T4776","obj":"T4776"},{"id":"R3532","pred":"prep","subj":"T4777","obj":"T4776"},{"id":"R3533","pred":"det","subj":"T4778","obj":"T4780"},{"id":"R3534","pred":"amod","subj":"T4779","obj":"T4780"},{"id":"R3535","pred":"pobj","subj":"T4780","obj":"T4777"},{"id":"R3536","pred":"prep","subj":"T4781","obj":"T4780"},{"id":"R3537","pred":"compound","subj":"T4782","obj":"T4783"},{"id":"R3538","pred":"pobj","subj":"T4783","obj":"T4781"},{"id":"R3539","pred":"prep","subj":"T4784","obj":"T4783"},{"id":"R3540","pred":"det","subj":"T4785","obj":"T4787"},{"id":"R3541","pred":"compound","subj":"T4786","obj":"T4787"},{"id":"R3542","pred":"pobj","subj":"T4787","obj":"T4784"},{"id":"R3543","pred":"prep","subj":"T4788","obj":"T4776"},{"id":"R3544","pred":"prep","subj":"T4789","obj":"T4788"},{"id":"R3545","pred":"pobj","subj":"T4790","obj":"T4789"},{"id":"R3546","pred":"punct","subj":"T4791","obj":"T4792"},{"id":"R3547","pred":"appos","subj":"T4792","obj":"T4790"},{"id":"R3548","pred":"punct","subj":"T4793","obj":"T4790"},{"id":"R3549","pred":"punct","subj":"T4794","obj":"T4795"},{"id":"R3550","pred":"amod","subj":"T4795","obj":"T4797"},{"id":"R3551","pred":"amod","subj":"T4796","obj":"T4797"},{"id":"R3552","pred":"pobj","subj":"T4797","obj":"T4789"},{"id":"R3553","pred":"punct","subj":"T4798","obj":"T4800"},{"id":"R3554","pred":"compound","subj":"T4799","obj":"T4800"},{"id":"R3555","pred":"appos","subj":"T4800","obj":"T4797"},{"id":"R3556","pred":"punct","subj":"T4801","obj":"T4800"},{"id":"R3557","pred":"punct","subj":"T4802","obj":"T4776"},{"id":"R3558","pred":"prep","subj":"T4803","obj":"T4809"},{"id":"R3559","pred":"det","subj":"T4804","obj":"T4805"},{"id":"R3560","pred":"pobj","subj":"T4805","obj":"T4803"},{"id":"R3561","pred":"punct","subj":"T4806","obj":"T4809"},{"id":"R3562","pred":"det","subj":"T4807","obj":"T4808"},{"id":"R3563","pred":"nsubj","subj":"T4808","obj":"T4809"},{"id":"R3564","pred":"ROOT","subj":"T4809","obj":"T4809"},{"id":"R3565","pred":"xcomp","subj":"T4810","obj":"T4809"},{"id":"R3566","pred":"amod","subj":"T4811","obj":"T4813"},{"id":"R3567","pred":"amod","subj":"T4812","obj":"T4813"},{"id":"R3568","pred":"nsubjpass","subj":"T4813","obj":"T4815"},{"id":"R3569","pred":"auxpass","subj":"T4814","obj":"T4815"},{"id":"R3570","pred":"ccomp","subj":"T4815","obj":"T4810"},{"id":"R3571","pred":"punct","subj":"T4816","obj":"T4809"},{"id":"R3572","pred":"nsubj","subj":"T4817","obj":"T4822"},{"id":"R3573","pred":"acl","subj":"T4818","obj":"T4817"},{"id":"R3574","pred":"prep","subj":"T4819","obj":"T4818"},{"id":"R3575","pred":"det","subj":"T4820","obj":"T4821"},{"id":"R3576","pred":"pobj","subj":"T4821","obj":"T4819"},{"id":"R3577","pred":"ROOT","subj":"T4822","obj":"T4822"},{"id":"R3578","pred":"aux","subj":"T4823","obj":"T4825"},{"id":"R3579","pred":"neg","subj":"T4824","obj":"T4825"},{"id":"R3580","pred":"conj","subj":"T4825","obj":"T4822"},{"id":"R3581","pred":"dobj","subj":"T4826","obj":"T4825"},{"id":"R3582","pred":"prep","subj":"T4827","obj":"T4825"},{"id":"R3583","pred":"det","subj":"T4828","obj":"T4829"},{"id":"R3584","pred":"pobj","subj":"T4829","obj":"T4827"},{"id":"R3585","pred":"cc","subj":"T4830","obj":"T4829"},{"id":"R3586","pred":"conj","subj":"T4831","obj":"T4829"},{"id":"R3587","pred":"prep","subj":"T4832","obj":"T4829"},{"id":"R3588","pred":"pobj","subj":"T4833","obj":"T4832"},{"id":"R3589","pred":"punct","subj":"T4834","obj":"T4836"},{"id":"R3590","pred":"compound","subj":"T4835","obj":"T4836"},{"id":"R3591","pred":"appos","subj":"T4836","obj":"T4833"},{"id":"R3592","pred":"punct","subj":"T4837","obj":"T4833"},{"id":"R3593","pred":"punct","subj":"T4838","obj":"T4822"},{"id":"R3594","pred":"nsubj","subj":"T4839","obj":"T4841"},{"id":"R3595","pred":"advmod","subj":"T4840","obj":"T4841"},{"id":"R3596","pred":"ROOT","subj":"T4841","obj":"T4841"},{"id":"R3597","pred":"mark","subj":"T4842","obj":"T4844"},{"id":"R3598","pred":"nsubj","subj":"T4843","obj":"T4844"},{"id":"R3599","pred":"ccomp","subj":"T4844","obj":"T4841"},{"id":"R3600","pred":"compound","subj":"T4845","obj":"T4846"},{"id":"R3601","pred":"dobj","subj":"T4846","obj":"T4844"},{"id":"R3602","pred":"prep","subj":"T4847","obj":"T4844"},{"id":"R3603","pred":"det","subj":"T4848","obj":"T4852"},{"id":"R3604","pred":"compound","subj":"T4849","obj":"T4852"},{"id":"R3605","pred":"compound","subj":"T4850","obj":"T4852"},{"id":"R3606","pred":"compound","subj":"T4851","obj":"T4852"},{"id":"R3607","pred":"pobj","subj":"T4852","obj":"T4847"},{"id":"R3608","pred":"punct","subj":"T4853","obj":"T4852"},{"id":"R3609","pred":"appos","subj":"T4854","obj":"T4852"},{"id":"R3610","pred":"nummod","subj":"T4855","obj":"T4854"},{"id":"R3611","pred":"punct","subj":"T4856","obj":"T4841"},{"id":"R3612","pred":"nsubjpass","subj":"T4857","obj":"T4861"},{"id":"R3613","pred":"nummod","subj":"T4858","obj":"T4859"},{"id":"R3614","pred":"nsubjpass","subj":"T4859","obj":"T4861"},{"id":"R3615","pred":"auxpass","subj":"T4860","obj":"T4861"},{"id":"R3616","pred":"ROOT","subj":"T4861","obj":"T4861"},{"id":"R3617","pred":"prep","subj":"T4862","obj":"T4861"},{"id":"R3618","pred":"preconj","subj":"T4863","obj":"T4866"},{"id":"R3619","pred":"det","subj":"T4864","obj":"T4866"},{"id":"R3620","pred":"compound","subj":"T4865","obj":"T4866"},{"id":"R3621","pred":"nsubj","subj":"T4866","obj":"T4870"},{"id":"R3622","pred":"cc","subj":"T4867","obj":"T4866"},{"id":"R3623","pred":"conj","subj":"T4868","obj":"T4866"},{"id":"R3624","pred":"amod","subj":"T4869","obj":"T4870"},{"id":"R3625","pred":"ROOT","subj":"T4870","obj":"T4870"},{"id":"R3626","pred":"punct","subj":"T4871","obj":"T4870"},{"id":"R3627","pred":"mark","subj":"T4872","obj":"T4873"},{"id":"R3628","pred":"advcl","subj":"T4873","obj":"T4884"},{"id":"R3629","pred":"prep","subj":"T4874","obj":"T4873"},{"id":"R3630","pred":"pobj","subj":"T4875","obj":"T4874"},{"id":"R3631","pred":"nummod","subj":"T4876","obj":"T4875"},{"id":"R3632","pred":"punct","subj":"T4877","obj":"T4875"},{"id":"R3633","pred":"compound","subj":"T4878","obj":"T4879"},{"id":"R3634","pred":"appos","subj":"T4879","obj":"T4875"},{"id":"R3635","pred":"prep","subj":"T4880","obj":"T4879"},{"id":"R3636","pred":"pobj","subj":"T4881","obj":"T4880"},{"id":"R3637","pred":"nummod","subj":"T4882","obj":"T4883"},{"id":"R3638","pred":"nsubj","subj":"T4883","obj":"T4884"},{"id":"R3639","pred":"ROOT","subj":"T4884","obj":"T4884"},{"id":"R3640","pred":"amod","subj":"T4885","obj":"T4887"},{"id":"R3641","pred":"compound","subj":"T4886","obj":"T4887"},{"id":"R3642","pred":"dobj","subj":"T4887","obj":"T4884"},{"id":"R3643","pred":"prep","subj":"T4888","obj":"T4884"},{"id":"R3644","pred":"pobj","subj":"T4889","obj":"T4888"},{"id":"R3645","pred":"prep","subj":"T4890","obj":"T4884"},{"id":"R3646","pred":"pobj","subj":"T4891","obj":"T4890"},{"id":"R3647","pred":"prep","subj":"T4892","obj":"T4891"},{"id":"R3648","pred":"amod","subj":"T4893","obj":"T4894"},{"id":"R3649","pred":"pobj","subj":"T4894","obj":"T4892"},{"id":"R3650","pred":"punct","subj":"T4895","obj":"T4884"},{"id":"R3651","pred":"advmod","subj":"T4896","obj":"T4900"},{"id":"R3652","pred":"punct","subj":"T4897","obj":"T4900"},{"id":"R3653","pred":"poss","subj":"T4898","obj":"T4899"},{"id":"R3654","pred":"nsubj","subj":"T4899","obj":"T4900"},{"id":"R3655","pred":"ROOT","subj":"T4900","obj":"T4900"},{"id":"R3656","pred":"mark","subj":"T4901","obj":"T4903"},{"id":"R3657","pred":"nsubj","subj":"T4902","obj":"T4903"},{"id":"R3658","pred":"ccomp","subj":"T4903","obj":"T4900"},{"id":"R3659","pred":"compound","subj":"T4904","obj":"T4906"},{"id":"R3660","pred":"compound","subj":"T4905","obj":"T4906"},{"id":"R3661","pred":"dobj","subj":"T4906","obj":"T4903"},{"id":"R3662","pred":"prep","subj":"T4907","obj":"T4906"},{"id":"R3663","pred":"pobj","subj":"T4908","obj":"T4907"},{"id":"R3664","pred":"punct","subj":"T4909","obj":"T4900"},{"id":"R17362","pred":"nsubj","subj":"T22818","obj":"T22819"},{"id":"R17363","pred":"ROOT","subj":"T22819","obj":"T22819"},{"id":"R17364","pred":"amod","subj":"T22820","obj":"T22822"},{"id":"R17365","pred":"amod","subj":"T22821","obj":"T22822"},{"id":"R17366","pred":"dobj","subj":"T22822","obj":"T22819"},{"id":"R17367","pred":"prep","subj":"T22823","obj":"T22822"},{"id":"R17368","pred":"pobj","subj":"T22824","obj":"T22823"},{"id":"R17369","pred":"punct","subj":"T22825","obj":"T22819"},{"id":"R17370","pred":"punct","subj":"T22826","obj":"T22831"},{"id":"R17371","pred":"nmod","subj":"T22827","obj":"T22829"},{"id":"R17372","pred":"punct","subj":"T22828","obj":"T22827"},{"id":"R17373","pred":"nsubjpass","subj":"T22829","obj":"T22831"},{"id":"R17374","pred":"auxpass","subj":"T22830","obj":"T22831"},{"id":"R17375","pred":"ROOT","subj":"T22831","obj":"T22831"},{"id":"R17376","pred":"prep","subj":"T22832","obj":"T22831"},{"id":"R17377","pred":"amod","subj":"T22833","obj":"T22834"},{"id":"R17378","pred":"pobj","subj":"T22834","obj":"T22832"},{"id":"R17379","pred":"conj","subj":"T22835","obj":"T22831"},{"id":"R17380","pred":"prep","subj":"T22836","obj":"T22835"},{"id":"R17381","pred":"cc","subj":"T22837","obj":"T22836"},{"id":"R17382","pred":"conj","subj":"T22838","obj":"T22836"},{"id":"R17383","pred":"pobj","subj":"T22839","obj":"T22838"},{"id":"R17384","pred":"prep","subj":"T22840","obj":"T22835"},{"id":"R17385","pred":"nummod","subj":"T22841","obj":"T22844"},{"id":"R17386","pred":"cc","subj":"T22842","obj":"T22841"},{"id":"R17387","pred":"conj","subj":"T22843","obj":"T22841"},{"id":"R17388","pred":"pobj","subj":"T22844","obj":"T22840"},{"id":"R17389","pred":"punct","subj":"T22845","obj":"T22831"},{"id":"R17390","pred":"compound","subj":"T22846","obj":"T22847"},{"id":"R17391","pred":"compound","subj":"T22847","obj":"T22849"},{"id":"R17392","pred":"amod","subj":"T22848","obj":"T22849"},{"id":"R17393","pred":"nsubjpass","subj":"T22849","obj":"T22851"},{"id":"R17394","pred":"auxpass","subj":"T22850","obj":"T22851"},{"id":"R17395","pred":"ROOT","subj":"T22851","obj":"T22851"},{"id":"R17396","pred":"agent","subj":"T22852","obj":"T22851"},{"id":"R17397","pred":"compound","subj":"T22853","obj":"T22854"},{"id":"R17398","pred":"pobj","subj":"T22854","obj":"T22852"},{"id":"R17399","pred":"conj","subj":"T22855","obj":"T22851"},{"id":"R17400","pred":"det","subj":"T22856","obj":"T22858"},{"id":"R17401","pred":"amod","subj":"T22857","obj":"T22858"},{"id":"R17402","pred":"attr","subj":"T22858","obj":"T22855"},{"id":"R17403","pred":"prep","subj":"T22859","obj":"T22858"},{"id":"R17404","pred":"nummod","subj":"T22860","obj":"T22862"},{"id":"R17405","pred":"amod","subj":"T22861","obj":"T22862"},{"id":"R17406","pred":"pobj","subj":"T22862","obj":"T22859"},{"id":"R17407","pred":"punct","subj":"T22863","obj":"T22851"},{"id":"R17408","pred":"ROOT","subj":"T22864","obj":"T22864"},{"id":"R17409","pred":"punct","subj":"T22865","obj":"T22864"},{"id":"R17410","pred":"acl","subj":"T22866","obj":"T22864"},{"id":"R17411","pred":"punct","subj":"T22867","obj":"T22864"},{"id":"R17412","pred":"punct","subj":"T22868","obj":"T22874"},{"id":"R17413","pred":"dep","subj":"T22869","obj":"T22874"},{"id":"R17414","pred":"punct","subj":"T22870","obj":"T22869"},{"id":"R17415","pred":"compound","subj":"T22871","obj":"T22872"},{"id":"R17416","pred":"nsubj","subj":"T22872","obj":"T22874"},{"id":"R17417","pred":"advmod","subj":"T22873","obj":"T22874"},{"id":"R17418","pred":"ROOT","subj":"T22874","obj":"T22874"},{"id":"R17419","pred":"prep","subj":"T22875","obj":"T22874"},{"id":"R17420","pred":"amod","subj":"T22876","obj":"T22879"},{"id":"R17421","pred":"cc","subj":"T22877","obj":"T22876"},{"id":"R17422","pred":"conj","subj":"T22878","obj":"T22876"},{"id":"R17423","pred":"pobj","subj":"T22879","obj":"T22875"},{"id":"R17424","pred":"auxpass","subj":"T22880","obj":"T22881"},{"id":"R17425","pred":"dep","subj":"T22881","obj":"T22874"},{"id":"R17426","pred":"prep","subj":"T22882","obj":"T22881"},{"id":"R17427","pred":"pobj","subj":"T22883","obj":"T22882"},{"id":"R17428","pred":"prep","subj":"T22884","obj":"T22883"},{"id":"R17429","pred":"compound","subj":"T22885","obj":"T22886"},{"id":"R17430","pred":"pobj","subj":"T22886","obj":"T22884"},{"id":"R17431","pred":"cc","subj":"T22887","obj":"T22881"},{"id":"R17432","pred":"conj","subj":"T22888","obj":"T22881"},{"id":"R17433","pred":"prep","subj":"T22889","obj":"T22888"},{"id":"R17434","pred":"nummod","subj":"T22890","obj":"T22891"},{"id":"R17435","pred":"pobj","subj":"T22891","obj":"T22889"},{"id":"R17436","pred":"advmod","subj":"T22892","obj":"T22888"},{"id":"R17437","pred":"det","subj":"T22893","obj":"T22894"},{"id":"R17438","pred":"pobj","subj":"T22894","obj":"T22892"},{"id":"R17439","pred":"prep","subj":"T22895","obj":"T22894"},{"id":"R17440","pred":"pobj","subj":"T22896","obj":"T22895"},{"id":"R17441","pred":"punct","subj":"T22897","obj":"T22874"},{"id":"R17442","pred":"amod","subj":"T22898","obj":"T22899"},{"id":"R17443","pred":"nsubjpass","subj":"T22899","obj":"T22901"},{"id":"R17444","pred":"auxpass","subj":"T22900","obj":"T22901"},{"id":"R17445","pred":"ROOT","subj":"T22901","obj":"T22901"},{"id":"R17446","pred":"prep","subj":"T22902","obj":"T22901"},{"id":"R17447","pred":"pcomp","subj":"T22903","obj":"T22902"},{"id":"R17448","pred":"det","subj":"T22904","obj":"T22905"},{"id":"R17449","pred":"dobj","subj":"T22905","obj":"T22903"},{"id":"R17450","pred":"prep","subj":"T22906","obj":"T22905"},{"id":"R17451","pred":"pobj","subj":"T22907","obj":"T22906"},{"id":"R17452","pred":"conj","subj":"T22908","obj":"T22901"},{"id":"R17453","pred":"prep","subj":"T22909","obj":"T22908"},{"id":"R17454","pred":"det","subj":"T22910","obj":"T22911"},{"id":"R17455","pred":"pobj","subj":"T22911","obj":"T22909"},{"id":"R17456","pred":"cc","subj":"T22912","obj":"T22911"},{"id":"R17457","pred":"conj","subj":"T22913","obj":"T22911"},{"id":"R17458","pred":"auxpass","subj":"T22914","obj":"T22915"},{"id":"R17459","pred":"ROOT","subj":"T22915","obj":"T22915"},{"id":"R17460","pred":"prep","subj":"T22916","obj":"T22915"},{"id":"R17461","pred":"compound","subj":"T22917","obj":"T22918"},{"id":"R17462","pred":"pobj","subj":"T22918","obj":"T22916"},{"id":"R17463","pred":"prep","subj":"T22919","obj":"T22918"},{"id":"R17464","pred":"compound","subj":"T22920","obj":"T22921"},{"id":"R17465","pred":"pobj","subj":"T22921","obj":"T22919"},{"id":"R17466","pred":"prep","subj":"T22922","obj":"T22921"},{"id":"R17467","pred":"pobj","subj":"T22923","obj":"T22922"},{"id":"R17468","pred":"prep","subj":"T22924","obj":"T22921"},{"id":"R17469","pred":"amod","subj":"T22925","obj":"T22928"},{"id":"R17470","pred":"amod","subj":"T22926","obj":"T22928"},{"id":"R17471","pred":"amod","subj":"T22927","obj":"T22928"},{"id":"R17472","pred":"pobj","subj":"T22928","obj":"T22924"},{"id":"R17473","pred":"punct","subj":"T22929","obj":"T22901"},{"id":"R17474","pred":"punct","subj":"T22930","obj":"T22933"},{"id":"R17475","pred":"nmod","subj":"T22931","obj":"T22933"},{"id":"R17476","pred":"punct","subj":"T22932","obj":"T22933"},{"id":"R17477","pred":"nsubjpass","subj":"T22933","obj":"T22938"},{"id":"R17478","pred":"nummod","subj":"T22934","obj":"T22935"},{"id":"R17479","pred":"nsubjpass","subj":"T22935","obj":"T22938"},{"id":"R17480","pred":"auxpass","subj":"T22936","obj":"T22938"},{"id":"R17481","pred":"advmod","subj":"T22937","obj":"T22938"},{"id":"R17482","pred":"ROOT","subj":"T22938","obj":"T22938"},{"id":"R17483","pred":"prep","subj":"T22939","obj":"T22938"},{"id":"R17484","pred":"pobj","subj":"T22940","obj":"T22939"},{"id":"R17485","pred":"cc","subj":"T22941","obj":"T22940"},{"id":"R17486","pred":"conj","subj":"T22942","obj":"T22940"},{"id":"R17487","pred":"conj","subj":"T22943","obj":"T22938"},{"id":"R17488","pred":"punct","subj":"T22944","obj":"T22938"},{"id":"R17489","pred":"nsubjpass","subj":"T22945","obj":"T22948"},{"id":"R17490","pred":"auxpass","subj":"T22946","obj":"T22948"},{"id":"R17491","pred":"advmod","subj":"T22947","obj":"T22948"},{"id":"R17492","pred":"ROOT","subj":"T22948","obj":"T22948"},{"id":"R17493","pred":"prep","subj":"T22949","obj":"T22948"},{"id":"R17494","pred":"nummod","subj":"T22950","obj":"T22951"},{"id":"R17495","pred":"pobj","subj":"T22951","obj":"T22949"},{"id":"R17496","pred":"advmod","subj":"T22952","obj":"T22948"},{"id":"R17497","pred":"prep","subj":"T22953","obj":"T22952"},{"id":"R17498","pred":"nummod","subj":"T22954","obj":"T22955"},{"id":"R17499","pred":"pobj","subj":"T22955","obj":"T22953"},{"id":"R17500","pred":"advcl","subj":"T22956","obj":"T22948"},{"id":"R17501","pred":"prep","subj":"T22957","obj":"T22956"},{"id":"R17502","pred":"amod","subj":"T22958","obj":"T22960"},{"id":"R17503","pred":"amod","subj":"T22959","obj":"T22960"},{"id":"R17504","pred":"pobj","subj":"T22960","obj":"T22957"},{"id":"R17505","pred":"punct","subj":"T22961","obj":"T22963"},{"id":"R17506","pred":"nummod","subj":"T22962","obj":"T22963"},{"id":"R17507","pred":"appos","subj":"T22963","obj":"T22960"},{"id":"R17508","pred":"punct","subj":"T22964","obj":"T22963"},{"id":"R17509","pred":"punct","subj":"T22965","obj":"T22948"},{"id":"R17510","pred":"compound","subj":"T22966","obj":"T22967"},{"id":"R17511","pred":"nsubjpass","subj":"T22967","obj":"T22969"},{"id":"R17512","pred":"auxpass","subj":"T22968","obj":"T22969"},{"id":"R17513","pred":"ROOT","subj":"T22969","obj":"T22969"},{"id":"R17514","pred":"aux","subj":"T22970","obj":"T22971"},{"id":"R17515","pred":"xcomp","subj":"T22971","obj":"T22969"},{"id":"R17516","pred":"compound","subj":"T22972","obj":"T22973"},{"id":"R17517","pred":"dobj","subj":"T22973","obj":"T22971"},{"id":"R17518","pred":"punct","subj":"T22974","obj":"T22969"},{"id":"R17519","pred":"nsubjpass","subj":"T22975","obj":"T22977"},{"id":"R17520","pred":"auxpass","subj":"T22976","obj":"T22977"},{"id":"R17521","pred":"ROOT","subj":"T22977","obj":"T22977"},{"id":"R17522","pred":"amod","subj":"T22978","obj":"T22980"},{"id":"R17523","pred":"compound","subj":"T22979","obj":"T22980"},{"id":"R17524","pred":"dobj","subj":"T22980","obj":"T22977"},{"id":"R17525","pred":"punct","subj":"T22981","obj":"T22977"},{"id":"R17526","pred":"prep","subj":"T22982","obj":"T22977"},{"id":"R17527","pred":"nummod","subj":"T22983","obj":"T22985"},{"id":"R17528","pred":"amod","subj":"T22984","obj":"T22985"},{"id":"R17529","pred":"pobj","subj":"T22985","obj":"T22982"},{"id":"R17530","pred":"punct","subj":"T22986","obj":"T22977"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}

{"project":"test2","denotations":[{"id":"T4653","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T4654","span":{"begin":148,"end":153},"obj":"Protein"},{"id":"T4655","span":{"begin":253,"end":258},"obj":"Protein"},{"id":"T4656","span":{"begin":409,"end":414},"obj":"Protein"},{"id":"T4657","span":{"begin":590,"end":595},"obj":"Protein"},{"id":"T4658","span":{"begin":938,"end":943},"obj":"Protein"},{"id":"T4659","span":{"begin":2016,"end":2021},"obj":"Protein"},{"id":"T4660","span":{"begin":2215,"end":2220},"obj":"Protein"},{"id":"T4661","span":{"begin":2250,"end":2259},"obj":"Positive_regulation"},{"id":"T4662","span":{"begin":2260,"end":2265},"obj":"Protein"},{"id":"T4663","span":{"begin":2364,"end":2369},"obj":"Protein"},{"id":"T22805","span":{"begin":1001,"end":1006},"obj":"Protein"},{"id":"T22806","span":{"begin":1128,"end":1133},"obj":"Protein"},{"id":"T22807","span":{"begin":1389,"end":1394},"obj":"Protein"},{"id":"T22808","span":{"begin":1842,"end":1847},"obj":"Protein"}],"relations":[{"id":"R3428","pred":"causeOf","subj":"T4660","obj":"T4661"}],"text":"M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7\nTo determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A). Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA. In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B). As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used. Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).\n10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.\n(A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes. NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments. NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium. NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct. Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml). Culture media was collected to measure SEAP production. Data are expressed mean ± S.E.M, for three independent experiments. We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7. RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct. As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells. Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages."}